MD04G1170000.v1.1

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0028703
Physical Location & Seq
Reverse (-)
26069174 .. 26071222
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1170000.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGGAGTGCTCTTCATTTACTTTTCCGGCCTTTCCTCCTTCCTCAACTCTAAGGCCAAAAGACATCTTCAAAGAGCGCATTCGTGTCCGAAGTTTTTACTCAGTTAACCATCTTTGTCATTGCATCAACCGCATCCATGCCGTGCCATCAAAATTACCACACAGCAACTGTTCCATTGTCCCAAATCAGCTTAGATCATTTTTGTTTTGCTCGAACACGAGTAAGTATATGAAAATGCAGGCTAGCATGAATATGATGGGGAAGTTCGGTGAGGGTACTAGTGATCAAGGGGCGGAAATAAAGGGAGCGAATTGGATGGGGGGGCTTGTGGCACTTGGTGAACTGTGGAACAATATTTGGGAGCAAAAAGAGGTTGCGGGCGAAGACAACGCTCACCATGAGGGTGAAAACGATGGTTGTGGAAGTGGTGGAGAGGAGGGTAGTTGTGTGGAAAGTTGTAGTTTAGAAGAAAATGATGGAGAAATGATAGACAGATCATACGATCCTGAATCTTTCTCGAGGTTTTTGGTTAGGGTACCATGGTCAGACACTAAGTTATTTTCTAAGCTAGCCTTCTTGTGTAACATGGCCAGTGTGATACCAGAGATCAAGCTTGTTTTATGGAAAGGTTCAACAATGACAAAAGTACTTGCAGCAGCAAGTGAAATGGAAAGACAAGAGATGGAAAATGACCTTAGATCACTCCACTACTCCTCGCCTTGTGAGTGGTTTGTTTGTGATGACTCCAGTACTTCTACTCGTTGCTTTGTGATTCAGGGAATGGACTCCGTTGCATCCCAGCAGGCATACCTCTTTTTCGAACCCACCAAATTTGAGGGGACGAATATACGTGTTCACAGAGGAATCTATGAATCGGCACAGGCTATGTACCAGCAATTCATGCCGGAAATATTGGAACACTTACATAGAAACGGTGAACTCGCAAAGCTTCAATTTACTGGCCATTGTCTTGGAGGAAGCATTGGTCTTTTAGTCCACATGATGCTCCTCACTAGGAAGGTTGTGAAACCCTCTACTCTTTTGCCAGTTGTCACATTTGGATCACCATTTGTGTTCTGCGGAGGCCACAAATTACTTGACCCACTAGGGTTGGATGCGACTGATCACATCCACTGTGTGATGATGCATAGAGATGTTGTCCCTAGAGCTTTCTCCTGCAAATACCCCAACCATGTGGCTACTCTCCTCAAGCATTTAAATGGTTCGTTTCAGTCACACCCTTGTCTGATAAAAAATGTAAGTACCAGTTTGATAAATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

428

Amino Acids

47.79

Weight (kDa)

6.34

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 257 - 393 1.4e-20 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028703)

Species Orthologous Gene IDs
malus_domestica MD04G1170000.v1.1
pyrus_communis pycom12g17270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 535
AciI CCGC 4 cut(s) 130, 293, 377, 1080
AclWI GGATC 2 cut(s) 497, 1069
AcoI YGGCCR 2 cut(s) 588, 961
AcsI RAATTY 2 cut(s) 830, 1276
AdeI CACNNNGTG 2 cut(s) 338, 1138
AfaI GTAC 6 cut(s) 277, 537, 648, 751, 890, 1264
AflIII ACRYGT 1 cut(s) 850
AgsI TTSAA 3 cut(s) 70, 633, 953
AhlI ACTAGT 1 cut(s) 278
AluBI AGCT 5 cut(s) 190, 568, 613, 949, 1169
AluI AGCT 5 cut(s) 190, 568, 613, 949, 1169
Alw21I GWGCWC 1 cut(s) 11
AlwI GGATC 2 cut(s) 497, 1069
AlwNI CAGNNNCTG 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 517
AoxI GGCC 5 cut(s) 27, 53, 588, 961, 1084
ApeKI GCWGC 2 cut(s) 653, 656
ApoI RAATTY 2 cut(s) 830, 1276
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 1 cut(s) 78
AsuHPI GGTGA 6 cut(s) 281, 350, 386, 416, 947, 1056
AsuII TTCGAA 1 cut(s) 819
AsuNHI GCTAGC 2 cut(s) 242, 568
AvaI CYCGRG 1 cut(s) 517
BaeI ACNNNNGTAYC 2 cut(s) 527, 560
BalI TGGCCA 2 cut(s) 590, 963
BanI GGYRCC 1 cut(s) 535
BauI CACGAG 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 2 cut(s) 665, 668
BccI CCATC 7 cut(s) 117, 154, 250, 310, 407, 470, 676
BceAI ACGGC 1 cut(s) 125
BclI TGATCA 2 cut(s) 283, 1123
BcuI ACTAGT 1 cut(s) 278
BfaI CTAG 6 cut(s) 243, 279, 569, 1014, 1106, 1164
BisI GCNGC 2 cut(s) 654, 657
BlsI GCNGC 2 cut(s) 655, 658
BmcAI AGTACT 2 cut(s) 648, 751
BmeT110I CYCGRG 1 cut(s) 517
BmiI GGNNCC 1 cut(s) 537
BmsI GCATC 6 cut(s) 132, 141, 803, 993, 1105, 1134
BmtI GCTAGC 2 cut(s) 246, 572
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 1 cut(s) 730
Bpu14I TTCGAA 1 cut(s) 819
BpuEI CTTGAG 1 cut(s) 1193
BsaAI YACGTR 1 cut(s) 851
BsaJI CCNNGG 1 cut(s) 539
Bse1I ACTGG 5 cut(s) 591, 747, 964, 1046, 1266
Bse3DI GCAATG 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 539
BseGI GGATG 5 cut(s) 132, 321, 794, 1120, 1128
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 5 cut(s) 591, 747, 964, 1046, 1266
BseRI GAGGAG 4 cut(s) 449, 703, 998, 1196
BseXI GCAGC 2 cut(s) 665, 668
BseYI CCCAGC 1 cut(s) 798
BshFI GGCC 5 cut(s) 29, 55, 590, 963, 1086
BshNI GGYRCC 1 cut(s) 535
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 517
BsiSI CCGG 2 cut(s) 26, 905
BslFI GGGAC 3 cut(s) 164, 853, 1145
BsmFI GGGAC 3 cut(s) 164, 853, 1145
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 5 cut(s) 29, 55, 590, 963, 1086
BsoBI CYCGRG 1 cut(s) 517
Bsp119I TTCGAA 1 cut(s) 819
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 8 cut(s) 194, 283, 494, 502, 606, 698, 1061, 1123
Bsp19I CCATGG 1 cut(s) 539
BspACI CCGC 4 cut(s) 130, 293, 377, 1080
BspANI GGCC 5 cut(s) 29, 55, 590, 963, 1086
BspCNI CTCAG 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 537
BspOI GCTAGC 2 cut(s) 246, 572
BspPI GGATC 2 cut(s) 497, 1069
BspQI GCTCTTC 1 cut(s) 16
BspT104I TTCGAA 1 cut(s) 819
BspT107I GGYRCC 1 cut(s) 535
BsrDI GCAATG 1 cut(s) 118
BsrI ACTGG 5 cut(s) 591, 747, 964, 1046, 1266
BssECI CCNNGG 1 cut(s) 539
BssMI GATC 8 cut(s) 194, 283, 494, 502, 606, 698, 1061, 1123
BssSI CACGAG 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 539
Bst2BI CACGAG 1 cut(s) 217
Bst4CI ACNGT 4 cut(s) 170, 345, 935, 1136
Bst6I CTCTTC 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 901
BstBAI YACGTR 1 cut(s) 851
BstBI TTCGAA 1 cut(s) 819
BstC8I GCNNGC 5 cut(s) 240, 244, 379, 570, 804
BstDEI CTNAG 6 cut(s) 50, 100, 191, 552, 564, 695
BstDSI CCRYGG 1 cut(s) 539
BstF5I GGATG 5 cut(s) 132, 321, 794, 1120, 1128
BstHHI GCGC 1 cut(s) 78
BstKTI GATC 8 cut(s) 197, 286, 497, 505, 609, 701, 1064, 1126
BstMBI GATC 8 cut(s) 194, 283, 494, 502, 606, 698, 1061, 1123
BstMWI GCNNNNNNNGC 2 cut(s) 129, 901
BstV1I GCAGC 2 cut(s) 665, 668
BstV2I GAAGAC 1 cut(s) 390
BstXI CCANNNNNNTGG 2 cut(s) 971, 1195
BsuRI GGCC 5 cut(s) 29, 55, 590, 963, 1086
BtgI CCRYGG 1 cut(s) 539
BtsCI GGATG 5 cut(s) 132, 321, 794, 1120, 1128
BtsIMutI CAGTG 2 cut(s) 598, 1132
Cac8I GCNNGC 5 cut(s) 240, 244, 379, 570, 804
CaiI CAGNNNCTG 1 cut(s) 168
CfoI GCGC 1 cut(s) 78
Csp6I GTAC 6 cut(s) 276, 536, 647, 750, 889, 1263
CspCI CAANNNNNGTGG 4 cut(s) 814, 849, 1092, 1127
CviAII CATG 8 cut(s) 137, 247, 398, 540, 586, 901, 1000, 1193
CviQI GTAC 6 cut(s) 276, 536, 647, 750, 889, 1263
DdeI CTNAG 6 cut(s) 50, 100, 191, 552, 564, 695
DpnI GATC 8 cut(s) 196, 285, 496, 504, 608, 700, 1063, 1125
DpnII GATC 8 cut(s) 194, 283, 494, 502, 606, 698, 1061, 1123
DraI TTTAAA 1 cut(s) 1218
DraIII CACNNNGTG 2 cut(s) 338, 1138
EaeI YGGCCR 2 cut(s) 588, 961
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
EciI GGCGGA 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 539
Eco88I CYCGRG 1 cut(s) 517
EcoT14I CCWWGG 1 cut(s) 539
EcoT22I ATGCAT 1 cut(s) 1149
ErhI CCWWGG 1 cut(s) 539
FaeI CATG 8 cut(s) 140, 250, 401, 543, 589, 904, 1003, 1196
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FaqI GGGAC 3 cut(s) 164, 853, 1145
FatI CATG 8 cut(s) 136, 246, 397, 539, 585, 900, 999, 1192
FauI CCCGC 1 cut(s) 370
FbaI TGATCA 2 cut(s) 283, 1123
Fnu4HI GCNGC 2 cut(s) 654, 657
FokI GGATG 5 cut(s) 119, 328, 781, 1115, 1127
Fsp4HI GCNGC 2 cut(s) 654, 657
FspBI CTAG 6 cut(s) 243, 279, 569, 1014, 1106, 1164
GlaI GCGC 1 cut(s) 77
GluI GCNGC 2 cut(s) 654, 657
GsaI CCCAGC 1 cut(s) 802
GsuI CTGGAG 1 cut(s) 730
HaeIII GGCC 5 cut(s) 29, 55, 590, 963, 1086
HapII CCGG 2 cut(s) 26, 905
HhaI GCGC 1 cut(s) 78
Hin1II CATG 8 cut(s) 140, 250, 401, 543, 589, 904, 1003, 1196
Hin6I GCGC 1 cut(s) 76
HinP1I GCGC 1 cut(s) 76
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 2 cut(s) 611, 947
HinfI GANTC 6 cut(s) 509, 743, 772, 785, 864, 872
HpaI GTTAAC 1 cut(s) 106
HpaII CCGG 2 cut(s) 26, 905
HphI GGTGA 6 cut(s) 281, 350, 386, 416, 947, 1056
Hpy166II GTNNAC 5 cut(s) 106, 341, 856, 938, 997
Hpy188I TCNGA 3 cut(s) 89, 547, 1248
Hpy188III TCNNGA 2 cut(s) 506, 517
Hpy8I GTNNAC 5 cut(s) 106, 341, 856, 938, 997
HpyAV CCTTC 3 cut(s) 48, 583, 1012
HpyCH4III ACNGT 4 cut(s) 170, 345, 935, 1136
HpyCH4IV ACGT 1 cut(s) 850
HpyCH4V TGCA 6 cut(s) 123, 238, 653, 794, 1147, 1179
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 901
HpyF3I CTNAG 6 cut(s) 50, 100, 191, 552, 564, 695
HpySE526I ACGT 1 cut(s) 850
Hsp92II CATG 8 cut(s) 140, 250, 401, 543, 589, 904, 1003, 1196
HspAI GCGC 1 cut(s) 76
KpnI GGTACC 1 cut(s) 539
Ksp22I TGATCA 2 cut(s) 283, 1123
KspAI GTTAAC 1 cut(s) 106
Kzo9I GATC 8 cut(s) 194, 283, 494, 502, 606, 698, 1061, 1123
LguI GCTCTTC 1 cut(s) 16
LmnI GCTCC 3 cut(s) 305, 361, 1011
Lsp1109I GCAGC 2 cut(s) 665, 668
LweI GCATC 6 cut(s) 132, 141, 803, 993, 1105, 1134
MaeI CTAG 6 cut(s) 243, 279, 569, 1014, 1106, 1164
MaeII ACGT 1 cut(s) 850
MaeIII GTNAC 3 cut(s) 581, 1051, 1233
MalI GATC 8 cut(s) 196, 285, 496, 504, 608, 700, 1063, 1125
MboI GATC 8 cut(s) 194, 283, 494, 502, 606, 698, 1061, 1123
MboII GAAGA 4 cut(s) 3, 58, 395, 479
MhlI GDGCHC 1 cut(s) 11
MlsI TGGCCA 2 cut(s) 590, 963
MluCI AATT 7 cut(s) 152, 310, 830, 896, 953, 1091, 1276
MluNI TGGCCA 2 cut(s) 590, 963
MlyI GAGTC 2 cut(s) 737, 779
MmeI TCCRAC 1 cut(s) 1092
Mox20I TGGCCA 2 cut(s) 590, 963
Mph1103I ATGCAT 1 cut(s) 1149
MscI TGGCCA 2 cut(s) 590, 963
MseI TTAA 2 cut(s) 105, 1217
MslI CAYNNNNRTG 4 cut(s) 251, 402, 1152, 1218
Msp20I TGGCCA 2 cut(s) 590, 963
MspI CCGG 2 cut(s) 26, 905
Mva1269I GAATGC 1 cut(s) 78
MwoI GCNNNNNNNGC 2 cut(s) 129, 901
NcoI CCATGG 1 cut(s) 539
NdeII GATC 8 cut(s) 194, 283, 494, 502, 606, 698, 1061, 1123
NheI GCTAGC 2 cut(s) 242, 568
NlaIII CATG 8 cut(s) 140, 250, 401, 543, 589, 904, 1003, 1196
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 2 cut(s) 1051, 1233
NsiI ATGCAT 1 cut(s) 1149
NspV TTCGAA 1 cut(s) 819
PaeR7I CTCGAG 1 cut(s) 517
PciSI GCTCTTC 1 cut(s) 16
PctI GAATGC 1 cut(s) 78
PfeI GAWTC 4 cut(s) 509, 772, 864, 872
PkrI GCNGC 2 cut(s) 655, 658
PleI GAGTC 2 cut(s) 737, 779
PpsI GAGTC 2 cut(s) 737, 779
Ppu21I YACGTR 1 cut(s) 851
PspFI CCCAGC 1 cut(s) 798
PspN4I GGNNCC 1 cut(s) 537
PstNI CAGNNNCTG 1 cut(s) 168
RsaI GTAC 6 cut(s) 277, 537, 648, 751, 890, 1264
RsaNI GTAC 6 cut(s) 276, 536, 647, 750, 889, 1263
RseI CAYNNNNRTG 4 cut(s) 251, 402, 1152, 1218
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 2 cut(s) 105, 1217
SatI GCNGC 2 cut(s) 654, 657
Sau3AI GATC 8 cut(s) 194, 283, 494, 502, 606, 698, 1061, 1123
ScaI AGTACT 2 cut(s) 648, 751
SchI GAGTC 2 cut(s) 737, 779
SduI GDGCHC 1 cut(s) 11
SfaNI GCATC 6 cut(s) 132, 141, 803, 993, 1105, 1134
Sfr274I CTCGAG 1 cut(s) 517
SfuI TTCGAA 1 cut(s) 819
SlaI CTCGAG 1 cut(s) 517
SmiI ATTTAAAT 1 cut(s) 1218
SmiMI CAYNNNNRTG 4 cut(s) 251, 402, 1152, 1218
SmlI CTYRAG 2 cut(s) 517, 1208
SmoI CTYRAG 2 cut(s) 517, 1208
SpeI ACTAGT 1 cut(s) 278
Sse9I AATT 7 cut(s) 152, 310, 830, 896, 953, 1091, 1276
SsiI CCGC 4 cut(s) 130, 293, 377, 1080
SspI AATATT 2 cut(s) 355, 912
SspMI CTAG 6 cut(s) 243, 279, 569, 1014, 1106, 1164
StyI CCWWGG 1 cut(s) 539
SwaI ATTTAAAT 1 cut(s) 1218
TaaI ACNGT 4 cut(s) 170, 345, 935, 1136
TaiI ACGT 1 cut(s) 853
TaqI TCGA 3 cut(s) 212, 518, 819
TasI AATT 7 cut(s) 152, 310, 830, 896, 953, 1091, 1276
TatI WGTACW 2 cut(s) 646, 749
TfiI GAWTC 4 cut(s) 509, 772, 864, 872
Tru1I TTAA 2 cut(s) 105, 1217
Tru9I TTAA 2 cut(s) 105, 1217
TscAI CASTG 2 cut(s) 598, 1139
TseFI GTSAC 2 cut(s) 1051, 1233
TseI GCWGC 2 cut(s) 653, 656
Tsp45I GTSAC 2 cut(s) 1051, 1233
TspDTI ATGAA 5 cut(s) 245, 263, 885, 889, 1269
TspGWI ACGGA 1 cut(s) 778
TspRI CASTG 2 cut(s) 598, 1139
XapI RAATTY 2 cut(s) 830, 1276
XhoI CTCGAG 1 cut(s) 517
XspI CTAG 6 cut(s) 243, 279, 569, 1014, 1106, 1164
ZrmI AGTACT 2 cut(s) 648, 751
Zsp2I ATGCAT 1 cut(s) 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.