MD04G1222000.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0020833
Physical Location & Seq
Forward (+)
30306471 .. 30311992
5522 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1222000.v1.1.491

Sequence Viewer

Length: 1821 bp
ATGGAGCAATATGAAGTTCTTGAGCAAATTGGGAAGGGGGCTTTTGGTTCTGCGCTTCTTGTCAGGCATAAGCATGAAAAGAAGAAGTATGTGTTGAAAAAGATTCGCCTTGCCCGCCAGACTGACAGATCACGGAGATCTGCACACCAGGAGATGGAGCTCATTTCCAAATTCAAAAATCCATTTATTGTGGAGTACAAAGATTCTTGGGTGGAAAAGGGTTGCTATGTGTGCATTATTATAGGTTATTGTGCAGGCGGAGACATGGCAGAAGCTATAAAAAAGGCCAACGGTCTTCTTTTCCCTGAAGAGAAGCTCTGTAAGTGGCTTGTCCAGCTTCTGATGGCACTCGATTACTTGCACAGGAATCATATTCTTCATCGCGATGTCAAGTGTTCTAATATATTCTTGACAAAAGATCAAGACATACGCCTTGGTGACTTTGGACTTGCCAAAAAGTTGACTTCGGATGATCTTGCCTCTTCGGTTGTCGGAACTCCTAGCTATATGTGTCCTGAGCTTCTTGCTGATATACCTTATGGTTCAAAATCAGATATTTGGTCCTTAGGATGCTGCATCTATGAAATGGCTTCTCATAGAGCCGCCTTTAAAGCGTTTGATATACAAGCACTGATTAACAAAATTAACAGGTCCATAGTGGCTCCCCTTCCCACAAAGTACACTGGTGCATTCCGAACACTTATTAAAAGCATGCTTCGGAAAAACCCAGAACTCAGGCCAAGTGCTGCAGAGCTGCTTGCTCACCCAGTCCTTCAGCCTCATGTTCATAAGGTTCATCTCAAACTCAATAACCCCAGACATGGAACTTTAGCGGTCAACTGGCCTGACACCGGTTACTTGAAGAAAACAAGATTTTCAGAGCCAGAAGATGTTCCTTTCTCCACTCCCCATTACCAGGAGAAGCGGCATACATTCAGCAATGATAGGACTTTAAATCCTAGTATATCTGGAGGACATTCTGTTGGTGGCACTGGCACCTATAGAAAAGCAGCCATACGCAAGCAACCAATTGCTTCCAAAACTTCAAATATCGTCAACAGTCGAGTTACTCCAGCAAAAGCTTCTGCCACTCCCAAGAGATTTATGGAGCCAAAGAATCGTGCATCGCTTCGTGTTTCTCGCACTCCGCAGAAGACAGTGAGTACAACTCGCAGAGCATCATTGCCATTAGCAAGAGCTGCAACTCAACAGTCCCCTTTCTGGCCCAATGTTGGTGTTCTTCACTGTATTAAATCTCCCGATGTTTCTGTCAATTCCCCTCGAATTGACAGGATAGCTGAATTCCCATTAGCCTCGTATGAAGAACAATTATACGAAGAACAGTTTCTGCCAATCCATAGAACCTCATCACCCTCTGTGCAAGGCTCGTCTGGTTCCCCACACTATGGTGACCGTTCAATGATGAAAGACAAATGCACAGTTCAGACAAACGACAGAGCCTCTCGCAAACTTAGTTTCACGGGTGTATGGCAGGGAATAGAACCTGGCATGTTCAAAGCTGCACCAGACAATCAAAGTGATGGATTTTCGGATCAAAATGCAACTGCTGGAGCATCGAGCCGGACCTCATCAAACACACGCCGCCGGCAGTTTGACACTTCATCATTCCAGCAAAGGGCTGAAGCTTTAGAAGGGCTGCTCGAATTCAGTGCAAGATTGCTCCAGAACGAACGATATGACGAGCTCGGGGTGTTGCTGAAGCCTTTTGGGCCAGGGAAGGTCTCTCCTAGGGAAACTGCTATTTGGTTGACTAAGAGCATCAAGCAAAATACTGTCAACCAGGATGATCAATTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

607

Amino Acids

68.06

Weight (kDa)

9.66

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 257 1.3e-63 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 253 1.2e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020833)

Species Orthologous Gene IDs
malus_domestica MD04G1222000.v1.1 MD12G1238200.v1.1
prunus_persica Prupe.6G341100_v2.0.a1 Prupe.6G341100_v2.0.a1
pyrus_communis pycom12g21890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 995
AccB7I CCANNNNNTGG 2 cut(s) 154, 1406
AccII CGCG 1 cut(s) 384
AciI CCGC 7 cut(s) 115, 258, 603, 833, 925, 1148, 1603
AclWI GGATC 1 cut(s) 1560
AcsI RAATTY 3 cut(s) 170, 1301, 1664
AcuI CTGAAG 4 cut(s) 327, 758, 1662, 1739
AfaI GTAC 3 cut(s) 197, 680, 1165
AfiI CCNNNNNNNGG 8 cut(s) 154, 821, 851, 916, 1221, 1232, 1406, 1636
AgeI ACCGGT 1 cut(s) 851
AgsI TTSAA 7 cut(s) 97, 175, 546, 862, 1047, 1419, 1516
AjnI CCWGG 5 cut(s) 147, 915, 1504, 1732, 1800
AleI CACNNNNGTG 1 cut(s) 1407
Alw21I GWGCWC 2 cut(s) 162, 1707
Alw26I GTCTC 2 cut(s) 255, 1747
AlwI GGATC 1 cut(s) 1560
AlwNI CAGNNNCTG 2 cut(s) 340, 1348
Ama87I CYCGRG 1 cut(s) 1706
AoxI GGCC 5 cut(s) 285, 737, 842, 1223, 1730
ApeKI GCWGC 7 cut(s) 573, 746, 754, 1010, 1199, 1520, 1657
ApoI RAATTY 3 cut(s) 170, 1301, 1664
AsiGI ACCGGT 1 cut(s) 851
Asp700I GAANNNNTTC 2 cut(s) 891, 1344
AspA2I CCTAGG 1 cut(s) 1748
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 5 cut(s) 561, 651, 1224, 1584, 1730
AsuHPI GGTGA 4 cut(s) 449, 755, 1362, 1421
AvaI CYCGRG 1 cut(s) 1706
AvaII GGWCC 3 cut(s) 561, 651, 1584
AvrII CCTAGG 1 cut(s) 1748
AxyI CCTNAGG 1 cut(s) 565
BanI GGYRCC 1 cut(s) 995
BanII GRGCYC 2 cut(s) 162, 1707
BbsI GAAGAC 2 cut(s) 287, 1160
Bbv12I GWGCWC 2 cut(s) 162, 1707
BbvI GCAGC 7 cut(s) 560, 733, 741, 1022, 1186, 1507, 1644
BccI CCATC 3 cut(s) 148, 337, 1535
BcgI CGANNNNNNTGC 2 cut(s) 1652, 1686
BciT130I CCWGG 5 cut(s) 149, 917, 1506, 1734, 1802
BclI TGATCA 1 cut(s) 1807
BcoDI GTCTC 2 cut(s) 255, 1747
BfaI CTAG 3 cut(s) 501, 960, 1749
BfmI CTRYAG 2 cut(s) 747, 1000
BglI GCCNNNNNGGC 1 cut(s) 1729
BglII AGATCT 1 cut(s) 137
BlnI CCTAGG 1 cut(s) 1748
Bme1390I CCNGG 5 cut(s) 149, 917, 1506, 1734, 1802
Bme18I GGWCC 3 cut(s) 561, 651, 1584
BmeT110I CYCGRG 1 cut(s) 1706
BmgT120I GGNCC 5 cut(s) 561, 651, 1224, 1584, 1730
BmiI GGNNCC 4 cut(s) 663, 997, 1110, 1396
BmrFI CCNGG 5 cut(s) 149, 917, 1506, 1734, 1802
BmrI ACTGGG 1 cut(s) 761
BmsI GCATC 6 cut(s) 560, 585, 1133, 1187, 1583, 1788
BmuI ACTGGG 1 cut(s) 761
BpiI GAAGAC 2 cut(s) 287, 1160
BplI GAGNNNNNCTC 2 cut(s) 1153, 1185
BpmI CTGGAG 4 cut(s) 990, 1056, 1590, 1667
Bpu10I CCTNAGC 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 1747
BsaJI CCNNGG 3 cut(s) 433, 1733, 1748
BsaWI WCCGGW 1 cut(s) 851
Bsc4I CCNNNNNNNGG 8 cut(s) 154, 821, 851, 916, 1221, 1232, 1406, 1636
Bse118I RCCGGY 2 cut(s) 851, 1605
Bse1I ACTGG 4 cut(s) 688, 767, 845, 997
Bse21I CCTNAGG 1 cut(s) 565
Bse3DI GCAATG 2 cut(s) 946, 1181
BseBI CCWGG 5 cut(s) 149, 917, 1506, 1734, 1802
BseDI CCNNGG 3 cut(s) 433, 1733, 1748
BseGI GGATG 3 cut(s) 475, 575, 1810
BseLI CCNNNNNNNGG 8 cut(s) 154, 821, 851, 916, 1221, 1232, 1406, 1636
BseMI GCAATG 2 cut(s) 946, 1181
BseMII CTCAG 2 cut(s) 507, 748
BseNI ACTGG 4 cut(s) 688, 767, 845, 997
BseXI GCAGC 7 cut(s) 560, 733, 741, 1022, 1186, 1507, 1644
BsgI GTGCAG 3 cut(s) 126, 273, 1506
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 5 cut(s) 287, 739, 844, 1225, 1732
BshNI GGYRCC 1 cut(s) 995
BshTI ACCGGT 1 cut(s) 851
BsiHKAI GWGCWC 2 cut(s) 162, 1707
BsiHKCI CYCGRG 1 cut(s) 1706
BsiSI CCGG 3 cut(s) 852, 1582, 1606
BslFI GGGAC 1 cut(s) 1198
BslI CCNNNNNNNGG 8 cut(s) 154, 821, 851, 916, 1221, 1232, 1406, 1636
BsmAI GTCTC 2 cut(s) 255, 1747
BsmFI GGGAC 1 cut(s) 1198
BsmI GAATGC 1 cut(s) 689
BsnI GGCC 5 cut(s) 287, 739, 844, 1225, 1732
Bso31I GGTCTC 1 cut(s) 1747
BsoBI CYCGRG 1 cut(s) 1706
Bsp1286I GDGCHC 2 cut(s) 162, 1707
Bsp143I GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
Bsp68I TCGCGA 1 cut(s) 384
BspACI CCGC 7 cut(s) 115, 258, 603, 833, 925, 1148, 1603
BspANI GGCC 5 cut(s) 287, 739, 844, 1225, 1732
BspCNI CTCAG 2 cut(s) 508, 747
BspFNI CGCG 1 cut(s) 384
BspLI GGNNCC 4 cut(s) 663, 997, 1110, 1396
BspMAI CTGCAG 1 cut(s) 751
BspPI GGATC 1 cut(s) 1560
BspT107I GGYRCC 1 cut(s) 995
BspTNI GGTCTC 1 cut(s) 1747
BsrDI GCAATG 2 cut(s) 946, 1181
BsrFI RCCGGY 2 cut(s) 851, 1605
BsrI ACTGG 4 cut(s) 688, 767, 845, 997
BssAI RCCGGY 2 cut(s) 851, 1605
BssECI CCNNGG 3 cut(s) 433, 1733, 1748
BssMI GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
BssT1I CCWWGG 2 cut(s) 433, 1748
Bst2UI CCWGG 5 cut(s) 149, 917, 1506, 1734, 1802
Bst4CI ACNGT 9 cut(s) 293, 1061, 1159, 1212, 1247, 1344, 1415, 1441, 1795
Bst6I CTCTTC 2 cut(s) 303, 487
BstAPI GCANNNNNTGC 1 cut(s) 1199
BstC8I GCNNGC 6 cut(s) 115, 256, 713, 759, 1022, 1607
BstDEI CTNAG 5 cut(s) 516, 565, 734, 1472, 1773
BstEII GGTNACC 1 cut(s) 1409
BstF5I GGATG 3 cut(s) 475, 575, 1810
BstFNI CGCG 1 cut(s) 384
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 6 cut(s) 131, 140, 421, 475, 1555, 1810
BstMAI GTCTC 2 cut(s) 255, 1747
BstMBI GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
BstMWI GCNNNNNNNGC 6 cut(s) 114, 231, 334, 611, 1199, 1729
BstNI CCWGG 5 cut(s) 149, 917, 1506, 1734, 1802
BstNSI RCATGY 2 cut(s) 715, 1513
BstPI GGTNACC 1 cut(s) 1409
BstSCI CCNGG 5 cut(s) 147, 915, 1504, 1732, 1800
BstSFI CTRYAG 2 cut(s) 747, 1000
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 7 cut(s) 560, 733, 741, 1022, 1186, 1507, 1644
BstV2I GAAGAC 2 cut(s) 287, 1160
BstX2I RGATCY 1 cut(s) 137
BstYI RGATCY 1 cut(s) 137
Bsu36I CCTNAGG 1 cut(s) 565
BsuRI GGCC 5 cut(s) 287, 739, 844, 1225, 1732
BtgZI GCGATG 3 cut(s) 365, 399, 1110
BtsCI GGATG 3 cut(s) 475, 575, 1810
BtsIMutI CAGTG 6 cut(s) 629, 681, 990, 1164, 1243, 1675
BtuMI TCGCGA 1 cut(s) 384
Cac8I GCNNGC 6 cut(s) 115, 256, 713, 759, 1022, 1607
CaiI CAGNNNCTG 2 cut(s) 340, 1348
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 2 cut(s) 851, 1605
Cfr13I GGNCC 5 cut(s) 561, 651, 1224, 1584, 1730
Csp6I GTAC 3 cut(s) 196, 679, 1164
CspAI ACCGGT 1 cut(s) 851
CviAII CATG 6 cut(s) 74, 265, 712, 782, 821, 1510
CviQI GTAC 3 cut(s) 196, 679, 1164
DdeI CTNAG 5 cut(s) 516, 565, 734, 1472, 1773
DpnI GATC 6 cut(s) 130, 139, 420, 474, 1554, 1809
DpnII GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
DraI TTTAAA 2 cut(s) 610, 954
Eam1104I CTCTTC 2 cut(s) 303, 487
EarI CTCTTC 2 cut(s) 303, 487
EciI GGCGGA 1 cut(s) 273
Ecl136II GAGCTC 2 cut(s) 160, 1705
Eco130I CCWWGG 2 cut(s) 433, 1748
Eco24I GRGCYC 2 cut(s) 162, 1707
Eco31I GGTCTC 1 cut(s) 1747
Eco47I GGWCC 3 cut(s) 561, 651, 1584
Eco53kI GAGCTC 2 cut(s) 160, 1705
Eco57I CTGAAG 4 cut(s) 327, 758, 1662, 1739
Eco81I CCTNAGG 1 cut(s) 565
Eco88I CYCGRG 1 cut(s) 1706
Eco91I GGTNACC 1 cut(s) 1409
EcoICRI GAGCTC 2 cut(s) 160, 1705
EcoO65I GGTNACC 1 cut(s) 1409
EcoRI GAATTC 2 cut(s) 1301, 1664
EcoRII CCWGG 5 cut(s) 147, 915, 1504, 1732, 1800
EcoT14I CCWWGG 2 cut(s) 433, 1748
EcoT38I GRGCYC 2 cut(s) 162, 1707
ErhI CCWWGG 2 cut(s) 433, 1748
FaeI CATG 6 cut(s) 77, 268, 715, 785, 824, 1513
FaqI GGGAC 1 cut(s) 1198
FatI CATG 6 cut(s) 73, 264, 711, 781, 820, 1509
FauI CCCGC 1 cut(s) 122
FbaI TGATCA 1 cut(s) 1807
FokI GGATG 3 cut(s) 482, 582, 1817
FriOI GRGCYC 2 cut(s) 162, 1707
FspBI CTAG 3 cut(s) 501, 960, 1749
GlaI GCGC 1 cut(s) 54
GsuI CTGGAG 4 cut(s) 990, 1056, 1590, 1667
HaeIII GGCC 5 cut(s) 287, 739, 844, 1225, 1732
HapII CCGG 3 cut(s) 852, 1582, 1606
HhaI GCGC 1 cut(s) 55
Hin1II CATG 6 cut(s) 77, 268, 715, 785, 824, 1513
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 5 cut(s) 462, 838, 1057, 1770, 1798
HindII GTYRAC 5 cut(s) 462, 838, 1057, 1770, 1798
HindIII AAGCTT 2 cut(s) 1080, 1644
HinfI GANTC 4 cut(s) 103, 203, 367, 1117
HpaII CCGG 3 cut(s) 852, 1582, 1606
HphI GGTGA 4 cut(s) 449, 755, 1362, 1421
Hpy166II GTNNAC 6 cut(s) 462, 681, 838, 1057, 1770, 1798
Hpy188I TCNGA 9 cut(s) 342, 469, 494, 553, 695, 720, 880, 1446, 1552
Hpy188III TCNNGA 8 cut(s) 20, 383, 409, 422, 515, 969, 1259, 1684
Hpy8I GTNNAC 6 cut(s) 462, 681, 838, 1057, 1770, 1798
HpyAV CCTTC 5 cut(s) 28, 677, 782, 1646, 1732
HpyCH4III ACNGT 9 cut(s) 293, 1061, 1159, 1212, 1247, 1344, 1415, 1441, 1795
HpyF10VI GCNNNNNNNGC 6 cut(s) 114, 231, 334, 611, 1199, 1729
HpyF3I CTNAG 5 cut(s) 516, 565, 734, 1472, 1773
Hsp92II CATG 6 cut(s) 77, 268, 715, 785, 824, 1513
HspAI GCGC 1 cut(s) 53
KroI GCCGGC 1 cut(s) 1605
KroNI GCCGGC 1 cut(s) 1607
Ksp22I TGATCA 1 cut(s) 1807
Kzo9I GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
LmnI GCTCC 6 cut(s) 4, 157, 667, 1108, 1571, 1686
Lsp1109I GCAGC 7 cut(s) 560, 733, 741, 1022, 1186, 1507, 1644
LweI GCATC 6 cut(s) 560, 585, 1133, 1187, 1583, 1788
MaeI CTAG 3 cut(s) 501, 960, 1749
MaeIII GTNAC 4 cut(s) 437, 854, 1066, 1409
MalI GATC 6 cut(s) 130, 139, 420, 474, 1554, 1809
MboI GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
MfeI CAATTG 1 cut(s) 1029
MflI RGATCY 1 cut(s) 137
MhlI GDGCHC 2 cut(s) 162, 1707
MmeI TCCRAC 1 cut(s) 472
MnlI CCTC 9 cut(s) 490, 789, 965, 1290, 1324, 1375, 1384, 1471, 1597
MroNI GCCGGC 1 cut(s) 1605
MroXI GAANNNNTTC 2 cut(s) 891, 1344
MseI TTAA 6 cut(s) 609, 636, 645, 705, 953, 1251
MslI CAYNNNNRTG 3 cut(s) 72, 384, 1407
MspI CCGG 3 cut(s) 852, 1582, 1606
MspR9I CCNGG 5 cut(s) 149, 917, 1506, 1734, 1802
MunI CAATTG 1 cut(s) 1029
Mva1269I GAATGC 1 cut(s) 689
MvaI CCWGG 5 cut(s) 149, 917, 1506, 1734, 1802
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 6 cut(s) 114, 231, 334, 611, 1199, 1729
NaeI GCCGGC 1 cut(s) 1607
NdeII GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
NgoMIV GCCGGC 1 cut(s) 1605
NlaIII CATG 6 cut(s) 77, 268, 715, 785, 824, 1513
NlaIV GGNNCC 4 cut(s) 663, 997, 1110, 1396
NmuCI GTSAC 2 cut(s) 437, 1409
NruI TCGCGA 1 cut(s) 384
NspI RCATGY 2 cut(s) 715, 1513
OliI CACNNNNGTG 1 cut(s) 1407
PaeI GCATGC 1 cut(s) 715
PctI GAATGC 1 cut(s) 689
PdiI GCCGGC 1 cut(s) 1607
PdmI GAANNNNTTC 2 cut(s) 891, 1344
PfeI GAWTC 4 cut(s) 103, 203, 367, 1117
PflMI CCANNNNNTGG 2 cut(s) 154, 1406
PinAI ACCGGT 1 cut(s) 851
Psp124BI GAGCTC 2 cut(s) 162, 1707
Psp6I CCWGG 5 cut(s) 147, 915, 1504, 1732, 1800
PspEI GGTNACC 1 cut(s) 1409
PspGI CCWGG 5 cut(s) 147, 915, 1504, 1732, 1800
PspN4I GGNNCC 4 cut(s) 663, 997, 1110, 1396
PspPI GGNCC 5 cut(s) 561, 651, 1224, 1584, 1730
PstI CTGCAG 1 cut(s) 751
PstNI CAGNNNCTG 2 cut(s) 340, 1348
PsuI RGATCY 1 cut(s) 137
RruI TCGCGA 1 cut(s) 384
RsaI GTAC 3 cut(s) 197, 680, 1165
RsaNI GTAC 3 cut(s) 196, 679, 1164
RseI CAYNNNNRTG 3 cut(s) 72, 384, 1407
SacI GAGCTC 2 cut(s) 162, 1707
SaqAI TTAA 6 cut(s) 609, 636, 645, 705, 953, 1251
Sau3AI GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
Sau96I GGNCC 5 cut(s) 561, 651, 1224, 1584, 1730
ScrFI CCNGG 5 cut(s) 149, 917, 1506, 1734, 1802
SduI GDGCHC 2 cut(s) 162, 1707
SfaNI GCATC 6 cut(s) 560, 585, 1133, 1187, 1583, 1788
SfcI CTRYAG 2 cut(s) 747, 1000
SinI GGWCC 3 cut(s) 561, 651, 1584
SmiMI CAYNNNNRTG 3 cut(s) 72, 384, 1407
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SphI GCATGC 1 cut(s) 715
SsiI CCGC 7 cut(s) 115, 258, 603, 833, 925, 1148, 1603
SspMI CTAG 3 cut(s) 501, 960, 1749
SstI GAGCTC 2 cut(s) 162, 1707
StyD4I CCNGG 5 cut(s) 147, 915, 1504, 1732, 1800
StyI CCWWGG 2 cut(s) 433, 1748
TaaI ACNGT 9 cut(s) 293, 1061, 1159, 1212, 1247, 1344, 1415, 1441, 1795
TaqI TCGA 5 cut(s) 351, 1063, 1282, 1577, 1662
TatI WGTACW 3 cut(s) 195, 678, 1163
TauI GCSGC 3 cut(s) 605, 928, 1605
TfiI GAWTC 4 cut(s) 103, 203, 367, 1117
Tru1I TTAA 6 cut(s) 609, 636, 645, 705, 953, 1251
Tru9I TTAA 6 cut(s) 609, 636, 645, 705, 953, 1251
TscAI CASTG 6 cut(s) 636, 688, 997, 1164, 1250, 1675
TseFI GTSAC 2 cut(s) 437, 1409
TseI GCWGC 7 cut(s) 573, 746, 754, 1010, 1199, 1520, 1657
Tsp45I GTSAC 2 cut(s) 437, 1409
TspDTI ATGAA 9 cut(s) 27, 90, 368, 597, 776, 785, 1335, 1439, 1611
TspGWI ACGGA 1 cut(s) 148
TspRI CASTG 6 cut(s) 636, 688, 997, 1164, 1250, 1675
Van91I CCANNNNNTGG 2 cut(s) 154, 1406
VpaK11BI GGWCC 3 cut(s) 561, 651, 1584
XapI RAATTY 3 cut(s) 170, 1301, 1664
XceI RCATGY 2 cut(s) 715, 1513
XcmI CCANNNNNNNNNTGG 1 cut(s) 1102
XmaJI CCTAGG 1 cut(s) 1748
XmnI GAANNNNTTC 2 cut(s) 891, 1344
XspI CTAG 3 cut(s) 501, 960, 1749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.