MD12G1238200.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0020833
Physical Location & Seq
Forward (+)
31152202 .. 31157530
5329 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1238200.v1.1.491

Sequence Viewer

Length: 1821 bp
ATGGAGCAATATGAAGTTCTTGAGCAAATCGGTAAAGGGGCTTTCGGTTCTGCGCTTCTTGTCAGGCATAAGCATGAAAAGAAGAAGTACGTGTTGAAAAAGATTCGTCTTGCTCGCCAGACTGATAGATCGCGGAGATCTGCACACCAGGAGATGGAGCTCATTTCCAAGTTCAAAAATCCATTTATTGTGGAGTACAAAGATTCTTGGGTGGAAAAGGGTTGCTATGTGTGCATTATCATCGGTTATTGCGCAGGAGGGGACATGGCAGAAGCTATAAAAAAGGCCAATGGTCTTCTTTTCCCTGAAGAGAAGCTCTGTAAGTGGCTTGTCCAACTTCTGATGGCGCTAGACTACTTGCACAGGAATCATATTCTTCATCGTGATGTCAAGTGTTCTAATATATTCTTGACAAAAGATCAAGACATACGCCTTGGTGACTTTGGACTTGCCAAAATGTTGACTTCAGACGATCTTGCCTCTTCGGTTGTCGGAACTCCTAGCTATATGTGTCCTGAGCTTCTTGCTGATATACCTTATGGTTCAAAATCAGATATTTGGTCTTTGGGATGCTGTATGTATGAAATGACTTCTCATAGGGCTGCATTTAAAGCGTTTGATATACAAGCACTGATTAACAAAATTAACAGGTCCATAGTGGCTCCCCTTCCCACAAAGTACACTGGTGCATTCCGAAGCCTTATTAAAAGCATGCTTCGGAAAAACCCAGAACTCAGGCCAAGTGCTGCTGAGCTGCTTGCTCACCCAGTCCTTCAGCCTCATGTTCACAAAGTTCATCTCAAACTCAATAACCCCAGACATGGAACTTTAGCGGTCAACTGGCCTGATACTGGTTACTTGAAGAAGACAAGGTTTTCAGAGCCAGAAGCAGTTCCTTTCTCCACTCGCCGTTACCAGGACAAGCTGCATACATTCAGCAATGATAGGACTTTAAATCCTAGTATATCTGGAGGACATTCTGTTGGTGGCACTGGAGCCTATAGAAAAGCAGCCATACGCAAGCAGCCAATTGCTTCCAAAACTTCAAATATCGTTAACAGTCGAGTTACTCCAGCAAAAGCTTCTGCCACTCCCAAGAGATTTATGGAGCCCAAGAATCGTGCATCGCTTCCCGTTTCACGCACACCGCAAAAGACAGTGAGTACAACTCGCAGAGCATCATTGCCATTAACAAGAGCTGCAACTCAACAGTCCCCTTTCTGGCCCAATGTTGGTGTTCTTCACTGTATTAAATCTCCTGACGTTTCTGTCAATTCCCCTCGAATTGACAGGATAGCTGAATTCCCATTAGCCTCATATGAAGAACAATTATACGAAGAACAGTTTCTGCCTATCCATAGAAACTCATCACCCTCAGTGCAAGGTTCGTCTGGTTCCCCCCACTCTGGTGACCGTTCAATGATGAAGGACAAATGCACCGTTCAGATAAGCGACAGAGCCTCTCGCAAACTTAGTTTCACAGGTGCATGGAAGGGAATTGAACCTGGCATGTTCAAAGCTGCACCAGACAATCAAAGCGATGAATGTTCGGATCAAAACGCAACTGCTGGAGCATCAAGCCGGACCTCATCAAACACGCGTCGCCAGCATTTTGACACCTCATCATTCCAGCAAAGGGCTGAAGCTTTAGAAGGGCTGCTCGAATTCAGTGCAAGATTGCTCCAGAACGAACGATATGACGAGCTTGGGGTGTTACTGAAGCCTTTTGGGCCAGGGAAGGTCTCTCCTAGGGAAACTGCTATTTGGTTGACTAAGAGCATCAAGCAAAATAATGTCGACCAGGATGATCAATTACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

607

Amino Acids

67.9

Weight (kDa)

9.58

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 257 2.2e-63 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 253 9e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020833)

Species Orthologous Gene IDs
malus_domestica MD04G1222000.v1.1 MD12G1238200.v1.1
prunus_persica Prupe.6G341100_v2.0.a1 Prupe.6G341100_v2.0.a1
pyrus_communis pycom12g21890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1268
Acc16I TGCGCA 1 cut(s) 253
AccB7I CCANNNNNTGG 1 cut(s) 154
AccI GTMKAC 1 cut(s) 1797
AccII CGCG 2 cut(s) 133, 1600
AciI CCGC 3 cut(s) 133, 833, 1148
AclWI GGATC 1 cut(s) 1560
AcsI RAATTY 2 cut(s) 1301, 1664
AcuI CTGAAG 5 cut(s) 327, 450, 758, 1662, 1739
AfaI GTAC 4 cut(s) 89, 197, 680, 1165
AfiI CCNNNNNNNGG 8 cut(s) 154, 821, 851, 916, 1221, 1232, 1406, 1636
AflIII ACRYGT 2 cut(s) 90, 1598
AgsI TTSAA 8 cut(s) 97, 175, 546, 862, 1047, 1419, 1502, 1516
AjnI CCWGG 5 cut(s) 147, 915, 1504, 1732, 1800
AleI CACNNNNGTG 1 cut(s) 1407
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 1747
AlwI GGATC 1 cut(s) 1560
AlwNI CAGNNNCTG 1 cut(s) 1348
AoxI GGCC 5 cut(s) 285, 737, 842, 1223, 1730
ApeKI GCWGC 9 cut(s) 602, 746, 754, 925, 1010, 1024, 1199, 1520, 1657
ApoI RAATTY 2 cut(s) 1301, 1664
Asp700I GAANNNNTTC 2 cut(s) 891, 1344
AspA2I CCTAGG 1 cut(s) 1748
AspLEI GCGC 3 cut(s) 55, 254, 349
AspS9I GGNCC 4 cut(s) 651, 1224, 1584, 1730
AsuHPI GGTGA 4 cut(s) 449, 755, 1362, 1421
AvaII GGWCC 2 cut(s) 651, 1584
AvrII CCTAGG 1 cut(s) 1748
BanII GRGCYC 2 cut(s) 162, 1113
BbsI GAAGAC 2 cut(s) 287, 872
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 9 cut(s) 589, 733, 741, 912, 1022, 1036, 1186, 1507, 1644
BccI CCATC 2 cut(s) 148, 337
BceAI ACGGC 1 cut(s) 894
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 1652, 1686
BciT130I CCWGG 5 cut(s) 149, 917, 1506, 1734, 1802
BclI TGATCA 1 cut(s) 1807
BcoDI GTCTC 1 cut(s) 1747
BfaI CTAG 4 cut(s) 350, 501, 960, 1749
BfmI CTRYAG 1 cut(s) 1000
BfoI RGCGCY 1 cut(s) 350
BglI GCCNNNNNGGC 1 cut(s) 1729
BglII AGATCT 1 cut(s) 137
BisI GCNGC 9 cut(s) 603, 747, 755, 926, 1011, 1025, 1200, 1521, 1658
BlnI CCTAGG 1 cut(s) 1748
BlpI GCTNAGC 1 cut(s) 750
BlsI GCNGC 9 cut(s) 604, 748, 756, 927, 1012, 1026, 1201, 1522, 1659
Bme1390I CCNGG 5 cut(s) 149, 917, 1506, 1734, 1802
Bme18I GGWCC 2 cut(s) 651, 1584
BmgT120I GGNCC 4 cut(s) 651, 1224, 1584, 1730
BmiI GGNNCC 4 cut(s) 663, 997, 1110, 1396
BmrFI CCNGG 5 cut(s) 149, 917, 1506, 1734, 1802
BmrI ACTGGG 1 cut(s) 761
BmsI GCATC 5 cut(s) 560, 1133, 1187, 1583, 1788
BmuI ACTGGG 1 cut(s) 761
BpiI GAAGAC 2 cut(s) 287, 872
BplI GAGNNNNNCTC 2 cut(s) 1153, 1185
BpmI CTGGAG 5 cut(s) 990, 1014, 1056, 1590, 1667
Bpu10I CCTNAGC 1 cut(s) 516
Bpu1102I GCTNAGC 1 cut(s) 750
BpuEI CTTGAG 1 cut(s) 41
BsaAI YACGTR 1 cut(s) 91
BsaI GGTCTC 1 cut(s) 1747
BsaJI CCNNGG 3 cut(s) 433, 1733, 1748
Bsc4I CCNNNNNNNGG 8 cut(s) 154, 821, 851, 916, 1221, 1232, 1406, 1636
Bse1I ACTGG 5 cut(s) 688, 767, 845, 856, 997
Bse3DI GCAATG 2 cut(s) 946, 1181
BseBI CCWGG 5 cut(s) 149, 917, 1506, 1734, 1802
BseDI CCNNGG 3 cut(s) 433, 1733, 1748
BseGI GGATG 2 cut(s) 575, 1810
BseLI CCNNNNNNNGG 8 cut(s) 154, 821, 851, 916, 1221, 1232, 1406, 1636
BseMI GCAATG 2 cut(s) 946, 1181
BseMII CTCAG 4 cut(s) 507, 741, 748, 1389
BseNI ACTGG 5 cut(s) 688, 767, 845, 856, 997
BseXI GCAGC 9 cut(s) 589, 733, 741, 912, 1022, 1036, 1186, 1507, 1644
BsgI GTGCAG 2 cut(s) 126, 1506
Bsh1236I CGCG 2 cut(s) 133, 1600
BshFI GGCC 5 cut(s) 287, 739, 844, 1225, 1732
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 1582
BslFI GGGAC 2 cut(s) 275, 1198
BslI CCNNNNNNNGG 8 cut(s) 154, 821, 851, 916, 1221, 1232, 1406, 1636
BsmAI GTCTC 1 cut(s) 1747
BsmFI GGGAC 2 cut(s) 275, 1198
BsmI GAATGC 1 cut(s) 689
BsnI GGCC 5 cut(s) 287, 739, 844, 1225, 1732
Bso31I GGTCTC 1 cut(s) 1747
Bsp1286I GDGCHC 2 cut(s) 162, 1113
Bsp143I GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
Bsp1720I GCTNAGC 1 cut(s) 750
BspACI CCGC 3 cut(s) 133, 833, 1148
BspANI GGCC 5 cut(s) 287, 739, 844, 1225, 1732
BspCNI CTCAG 4 cut(s) 508, 742, 747, 1388
BspFNI CGCG 2 cut(s) 133, 1600
BspLI GGNNCC 4 cut(s) 663, 997, 1110, 1396
BspPI GGATC 1 cut(s) 1560
BspTNI GGTCTC 1 cut(s) 1747
BsrDI GCAATG 2 cut(s) 946, 1181
BsrI ACTGG 5 cut(s) 688, 767, 845, 856, 997
BssECI CCNNGG 3 cut(s) 433, 1733, 1748
BssMI GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
BssT1I CCWWGG 2 cut(s) 433, 1748
Bst2UI CCWGG 5 cut(s) 149, 917, 1506, 1734, 1802
Bst4CI ACNGT 7 cut(s) 1061, 1159, 1212, 1247, 1344, 1415, 1441
Bst6I CTCTTC 2 cut(s) 303, 487
BstBAI YACGTR 1 cut(s) 91
BstC8I GCNNGC 5 cut(s) 115, 713, 759, 1022, 1607
BstDEI CTNAG 6 cut(s) 516, 734, 750, 1375, 1472, 1773
BstEII GGTNACC 1 cut(s) 1409
BstF5I GGATG 2 cut(s) 575, 1810
BstFNI CGCG 2 cut(s) 133, 1600
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 3 cut(s) 55, 254, 349
BstKTI GATC 6 cut(s) 131, 140, 421, 475, 1555, 1810
BstMAI GTCTC 1 cut(s) 1747
BstMBI GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
BstMWI GCNNNNNNNGC 4 cut(s) 231, 611, 1606, 1729
BstNI CCWGG 5 cut(s) 149, 917, 1506, 1734, 1802
BstNSI RCATGY 2 cut(s) 715, 1513
BstPI GGTNACC 1 cut(s) 1409
BstSCI CCNGG 5 cut(s) 147, 915, 1504, 1732, 1800
BstSFI CTRYAG 1 cut(s) 1000
BstUI CGCG 2 cut(s) 133, 1600
BstV1I GCAGC 9 cut(s) 589, 733, 741, 912, 1022, 1036, 1186, 1507, 1644
BstV2I GAAGAC 2 cut(s) 287, 872
BstX2I RGATCY 1 cut(s) 137
BstYI RGATCY 1 cut(s) 137
BsuRI GGCC 5 cut(s) 287, 739, 844, 1225, 1732
BtgZI GCGATG 2 cut(s) 1110, 1554
BtsCI GGATG 2 cut(s) 575, 1810
BtsIMutI CAGTG 7 cut(s) 629, 681, 990, 1164, 1243, 1383, 1675
Cac8I GCNNGC 5 cut(s) 115, 713, 759, 1022, 1607
CaiI CAGNNNCTG 1 cut(s) 1348
CfoI GCGC 3 cut(s) 55, 254, 349
Cfr13I GGNCC 4 cut(s) 651, 1224, 1584, 1730
CseI GACGC 1 cut(s) 1589
Csp6I GTAC 4 cut(s) 88, 196, 679, 1164
CviAII CATG 7 cut(s) 74, 265, 712, 782, 821, 1488, 1510
CviQI GTAC 4 cut(s) 88, 196, 679, 1164
DdeI CTNAG 6 cut(s) 516, 734, 750, 1375, 1472, 1773
DpnI GATC 6 cut(s) 130, 139, 420, 474, 1554, 1809
DpnII GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
DraI TTTAAA 2 cut(s) 610, 954
DrdI GACNNNNNNGTC 1 cut(s) 1268
DseDI GACNNNNNNGTC 1 cut(s) 1268
Eam1104I CTCTTC 2 cut(s) 303, 487
EarI CTCTTC 2 cut(s) 303, 487
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 2 cut(s) 433, 1748
Eco24I GRGCYC 2 cut(s) 162, 1113
Eco31I GGTCTC 1 cut(s) 1747
Eco47I GGWCC 2 cut(s) 651, 1584
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 5 cut(s) 327, 450, 758, 1662, 1739
Eco91I GGTNACC 1 cut(s) 1409
EcoICRI GAGCTC 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 1409
EcoRI GAATTC 2 cut(s) 1301, 1664
EcoRII CCWGG 5 cut(s) 147, 915, 1504, 1732, 1800
EcoT14I CCWWGG 2 cut(s) 433, 1748
EcoT38I GRGCYC 2 cut(s) 162, 1113
ErhI CCWWGG 2 cut(s) 433, 1748
FaeI CATG 7 cut(s) 77, 268, 715, 785, 824, 1491, 1513
FaqI GGGAC 2 cut(s) 275, 1198
FatI CATG 7 cut(s) 73, 264, 711, 781, 820, 1487, 1509
FauNDI CATATG 1 cut(s) 1318
FbaI TGATCA 1 cut(s) 1807
FblI GTMKAC 1 cut(s) 1797
Fnu4HI GCNGC 9 cut(s) 603, 747, 755, 926, 1011, 1025, 1200, 1521, 1658
FokI GGATG 2 cut(s) 582, 1817
FriOI GRGCYC 2 cut(s) 162, 1113
Fsp4HI GCNGC 9 cut(s) 603, 747, 755, 926, 1011, 1025, 1200, 1521, 1658
FspBI CTAG 4 cut(s) 350, 501, 960, 1749
FspI TGCGCA 1 cut(s) 253
GlaI GCGC 3 cut(s) 54, 253, 348
GluI GCNGC 9 cut(s) 603, 747, 755, 926, 1011, 1025, 1200, 1521, 1658
GsuI CTGGAG 5 cut(s) 990, 1014, 1056, 1590, 1667
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 5 cut(s) 287, 739, 844, 1225, 1732
HapII CCGG 1 cut(s) 1582
HgaI GACGC 1 cut(s) 1589
HhaI GCGC 3 cut(s) 55, 254, 349
Hin1II CATG 7 cut(s) 77, 268, 715, 785, 824, 1491, 1513
Hin6I GCGC 3 cut(s) 53, 252, 347
HinP1I GCGC 3 cut(s) 53, 252, 347
HincII GTYRAC 5 cut(s) 462, 838, 1057, 1770, 1798
HindII GTYRAC 5 cut(s) 462, 838, 1057, 1770, 1798
HindIII AAGCTT 2 cut(s) 1080, 1644
HinfI GANTC 4 cut(s) 103, 203, 367, 1117
HpaI GTTAAC 1 cut(s) 1057
HpaII CCGG 1 cut(s) 1582
HphI GGTGA 4 cut(s) 449, 755, 1362, 1421
Hpy166II GTNNAC 7 cut(s) 462, 681, 787, 838, 1057, 1770, 1798
Hpy188I TCNGA 9 cut(s) 342, 469, 494, 553, 695, 720, 880, 1446, 1552
Hpy188III TCNNGA 8 cut(s) 20, 383, 409, 422, 515, 969, 1259, 1684
Hpy8I GTNNAC 7 cut(s) 462, 681, 787, 838, 1057, 1770, 1798
Hpy99I CGWCG 1 cut(s) 1605
HpyAV CCTTC 6 cut(s) 677, 782, 1420, 1486, 1646, 1732
HpyCH4III ACNGT 7 cut(s) 1061, 1159, 1212, 1247, 1344, 1415, 1441
HpyCH4IV ACGT 2 cut(s) 90, 1263
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 611, 1606, 1729
HpyF3I CTNAG 6 cut(s) 516, 734, 750, 1375, 1472, 1773
HpySE526I ACGT 2 cut(s) 90, 1263
Hsp92II CATG 7 cut(s) 77, 268, 715, 785, 824, 1491, 1513
HspAI GCGC 3 cut(s) 53, 252, 347
Ksp22I TGATCA 1 cut(s) 1807
KspAI GTTAAC 1 cut(s) 1057
Kzo9I GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
LmnI GCTCC 7 cut(s) 4, 157, 667, 995, 1108, 1571, 1686
Lsp1109I GCAGC 9 cut(s) 589, 733, 741, 912, 1022, 1036, 1186, 1507, 1644
LweI GCATC 5 cut(s) 560, 1133, 1187, 1583, 1788
MaeI CTAG 4 cut(s) 350, 501, 960, 1749
MaeII ACGT 2 cut(s) 90, 1263
MaeIII GTNAC 6 cut(s) 437, 854, 911, 1066, 1409, 1713
MalI GATC 6 cut(s) 130, 139, 420, 474, 1554, 1809
MboI GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
MfeI CAATTG 1 cut(s) 1029
MflI RGATCY 1 cut(s) 137
MhlI GDGCHC 2 cut(s) 162, 1113
MluCI AATT 9 cut(s) 642, 1029, 1273, 1284, 1301, 1328, 1497, 1664, 1811
MluI ACGCGT 1 cut(s) 1598
MmeI TCCRAC 2 cut(s) 358, 472
MroXI GAANNNNTTC 2 cut(s) 891, 1344
MseI TTAA 8 cut(s) 609, 636, 645, 705, 953, 1056, 1190, 1251
MslI CAYNNNNRTG 3 cut(s) 72, 384, 1407
MspI CCGG 1 cut(s) 1582
MspR9I CCNGG 5 cut(s) 149, 917, 1506, 1734, 1802
MunI CAATTG 1 cut(s) 1029
Mva1269I GAATGC 1 cut(s) 689
MvaI CCWGG 5 cut(s) 149, 917, 1506, 1734, 1802
MvnI CGCG 2 cut(s) 133, 1600
MwoI GCNNNNNNNGC 4 cut(s) 231, 611, 1606, 1729
NdeI CATATG 1 cut(s) 1318
NdeII GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
NlaIII CATG 7 cut(s) 77, 268, 715, 785, 824, 1491, 1513
NlaIV GGNNCC 4 cut(s) 663, 997, 1110, 1396
NmuCI GTSAC 2 cut(s) 437, 1409
NsbI TGCGCA 1 cut(s) 253
NspI RCATGY 2 cut(s) 715, 1513
OliI CACNNNNGTG 1 cut(s) 1407
PaeI GCATGC 1 cut(s) 715
PctI GAATGC 1 cut(s) 689
PdmI GAANNNNTTC 2 cut(s) 891, 1344
PfeI GAWTC 4 cut(s) 103, 203, 367, 1117
PflMI CCANNNNNTGG 1 cut(s) 154
PkrI GCNGC 9 cut(s) 604, 748, 756, 927, 1012, 1026, 1201, 1522, 1659
Ppu21I YACGTR 1 cut(s) 91
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 5 cut(s) 147, 915, 1504, 1732, 1800
PspEI GGTNACC 1 cut(s) 1409
PspGI CCWGG 5 cut(s) 147, 915, 1504, 1732, 1800
PspN4I GGNNCC 4 cut(s) 663, 997, 1110, 1396
PspPI GGNCC 4 cut(s) 651, 1224, 1584, 1730
PstNI CAGNNNCTG 1 cut(s) 1348
PsuI RGATCY 1 cut(s) 137
RsaI GTAC 4 cut(s) 89, 197, 680, 1165
RsaNI GTAC 4 cut(s) 88, 196, 679, 1164
RseI CAYNNNNRTG 3 cut(s) 72, 384, 1407
SacI GAGCTC 1 cut(s) 162
SalI GTCGAC 1 cut(s) 1796
SaqAI TTAA 8 cut(s) 609, 636, 645, 705, 953, 1056, 1190, 1251
SatI GCNGC 9 cut(s) 603, 747, 755, 926, 1011, 1025, 1200, 1521, 1658
Sau3AI GATC 6 cut(s) 128, 137, 418, 472, 1552, 1807
Sau96I GGNCC 4 cut(s) 651, 1224, 1584, 1730
ScrFI CCNGG 5 cut(s) 149, 917, 1506, 1734, 1802
SduI GDGCHC 2 cut(s) 162, 1113
SfaNI GCATC 5 cut(s) 560, 1133, 1187, 1583, 1788
SfcI CTRYAG 1 cut(s) 1000
SinI GGWCC 2 cut(s) 651, 1584
SmiMI CAYNNNNRTG 3 cut(s) 72, 384, 1407
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SphI GCATGC 1 cut(s) 715
Sse9I AATT 9 cut(s) 642, 1029, 1273, 1284, 1301, 1328, 1497, 1664, 1811
SsiI CCGC 3 cut(s) 133, 833, 1148
SspMI CTAG 4 cut(s) 350, 501, 960, 1749
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 5 cut(s) 147, 915, 1504, 1732, 1800
StyI CCWWGG 2 cut(s) 433, 1748
TaaI ACNGT 7 cut(s) 1061, 1159, 1212, 1247, 1344, 1415, 1441
TaiI ACGT 2 cut(s) 93, 1266
TaqI TCGA 4 cut(s) 1063, 1282, 1662, 1797
TasI AATT 9 cut(s) 642, 1029, 1273, 1284, 1301, 1328, 1497, 1664, 1811
TatI WGTACW 3 cut(s) 195, 678, 1163
TfiI GAWTC 4 cut(s) 103, 203, 367, 1117
Tru1I TTAA 8 cut(s) 609, 636, 645, 705, 953, 1056, 1190, 1251
Tru9I TTAA 8 cut(s) 609, 636, 645, 705, 953, 1056, 1190, 1251
TscAI CASTG 7 cut(s) 636, 688, 997, 1164, 1250, 1383, 1675
TseFI GTSAC 2 cut(s) 437, 1409
TseI GCWGC 9 cut(s) 602, 746, 754, 925, 1010, 1024, 1199, 1520, 1657
Tsp45I GTSAC 2 cut(s) 437, 1409
TspDTI ATGAA 8 cut(s) 27, 90, 368, 597, 785, 1335, 1439, 1557
TspRI CASTG 7 cut(s) 636, 688, 997, 1164, 1250, 1383, 1675
Van91I CCANNNNNTGG 1 cut(s) 154
VpaK11BI GGWCC 2 cut(s) 651, 1584
XapI RAATTY 2 cut(s) 1301, 1664
XceI RCATGY 2 cut(s) 715, 1513
XcmI CCANNNNNNNNNTGG 1 cut(s) 1102
XmaJI CCTAGG 1 cut(s) 1748
XmiI GTMKAC 1 cut(s) 1797
XmnI GAANNNNTTC 2 cut(s) 891, 1344
XspI CTAG 4 cut(s) 350, 501, 960, 1749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.