MD05G1135400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024223
Physical Location & Seq
Forward (+)
26352884 .. 26354243
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1135400.v1.1.491

Sequence Viewer

Length: 234 bp
ATGTACCCGCCACCGCCACCGCTCCCGTACAACCCTTCGCACAAGTCCCCGATCTCCGTCGGCGCCGTCGGGTCCTCCCCGGCGTACCACGCATTCACCAGCGGGTTGGACGCAAGCTCAGCGAGCTCGTGCCCAATCACGCTGATCATCCCGTCCACGCCCACGTCCCTGTTGGGCGGGGACAGCGCCCCGGGCCCGCCGCCACCCATGTACCCTGGGATAGCGAACGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

78

Amino Acids

7.61

Weight (kDa)

5.94

Isoelectric Point (pI)

75.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024223)

Species Orthologous Gene IDs
malus_domestica MD05G1135400.v1.1 MD10G1140700.v1.1
pyrus_communis pycom05g22970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 62
AccBSI CCGCTC 1 cut(s) 22
AciI CCGC 7 cut(s) 8, 14, 20, 102, 177, 197, 200
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 4 cut(s) 5, 29, 86, 212
AjiI CACGTC 1 cut(s) 165
AjnI CCWGG 1 cut(s) 214
AluBI AGCT 2 cut(s) 117, 126
AluI AGCT 2 cut(s) 117, 126
Alw21I GWGCWC 1 cut(s) 128
Ama87I CYCGRG 1 cut(s) 190
AoxI GGCC 1 cut(s) 193
ApaI GGGCCC 1 cut(s) 197
AspLEI GCGC 2 cut(s) 65, 188
AspS9I GGNCC 3 cut(s) 72, 193, 194
AsuC2I CCSGG 3 cut(s) 80, 191, 192
AsuHPI GGTGA 1 cut(s) 88
AvaI CYCGRG 1 cut(s) 190
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 2 cut(s) 134, 197
BaeI ACNNNNGTAYC 2 cut(s) 194, 227
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 2 cut(s) 128, 197
BauI CACGAG 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 216
BclI TGATCA 1 cut(s) 144
BcnI CCSGG 3 cut(s) 80, 191, 192
BfoI RGCGCY 2 cut(s) 66, 189
BisI GCNGC 1 cut(s) 200
BlpI GCTNAGC 1 cut(s) 118
BlsI GCNGC 1 cut(s) 201
Bme1390I CCNGG 4 cut(s) 80, 191, 192, 216
Bme18I GGWCC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 190
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 3 cut(s) 72, 193, 194
BmiI GGNNCC 3 cut(s) 64, 73, 195
BmrFI CCNGG 4 cut(s) 80, 191, 192, 216
Bpu1102I GCTNAGC 1 cut(s) 118
BpuMI CCSGG 3 cut(s) 80, 191, 192
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 5 cut(s) 78, 189, 190, 214, 215
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 5 cut(s) 78, 189, 190, 214, 215
BseGI GGATG 1 cut(s) 147
BseMII CTCAG 1 cut(s) 132
BseSI GKGCMC 2 cut(s) 134, 197
BshFI GGCC 1 cut(s) 195
BshNI GGYRCC 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 128
BsiHKCI CYCGRG 1 cut(s) 190
BsiSI CCGG 2 cut(s) 80, 191
BslFI GGGAC 3 cut(s) 31, 151, 194
BsmFI GGGAC 3 cut(s) 31, 151, 194
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 190
Bsp120I GGGCCC 1 cut(s) 193
Bsp1286I GDGCHC 3 cut(s) 128, 134, 197
Bsp143I GATC 2 cut(s) 51, 144
Bsp1720I GCTNAGC 1 cut(s) 118
BspACI CCGC 7 cut(s) 8, 14, 20, 102, 177, 197, 200
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 1 cut(s) 131
BspLI GGNNCC 3 cut(s) 64, 73, 195
BspT107I GGYRCC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 22
BssECI CCNNGG 5 cut(s) 78, 189, 190, 214, 215
BssMI GATC 2 cut(s) 51, 144
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 127
Bst2BI CACGAG 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 216
BstACI GRCGYC 1 cut(s) 63
BstC8I GCNNGC 3 cut(s) 115, 124, 197
BstDEI CTNAG 1 cut(s) 118
BstF5I GGATG 1 cut(s) 147
BstH2I RGCGCY 2 cut(s) 66, 189
BstHHI GCGC 2 cut(s) 65, 188
BstKTI GATC 2 cut(s) 54, 147
BstMBI GATC 2 cut(s) 51, 144
BstMWI GCNNNNNNNGC 5 cut(s) 89, 119, 123, 183, 192
BstNI CCWGG 1 cut(s) 216
BstSCI CCNGG 4 cut(s) 78, 189, 190, 214
BstSLI GKGCMC 2 cut(s) 134, 197
BstXI CCANNNNNNTGG 1 cut(s) 106
BsuRI GGCC 1 cut(s) 195
BtrI CACGTC 1 cut(s) 165
BtsCI GGATG 1 cut(s) 147
Cac8I GCNNGC 3 cut(s) 115, 124, 197
CfoI GCGC 2 cut(s) 65, 188
Cfr13I GGNCC 3 cut(s) 72, 193, 194
Cfr9I CCCGGG 1 cut(s) 190
CseI GACGC 1 cut(s) 119
Csp6I GTAC 4 cut(s) 4, 28, 85, 211
CviAII CATG 1 cut(s) 208
CviJI RGCY 3 cut(s) 117, 126, 195
CviKI_1 RGCY 3 cut(s) 117, 126, 195
CviQI GTAC 4 cut(s) 4, 28, 85, 211
DdeI CTNAG 1 cut(s) 118
DinI GGCGCC 1 cut(s) 64
DpnI GATC 2 cut(s) 53, 146
DpnII GATC 2 cut(s) 51, 144
Ecl136II GAGCTC 1 cut(s) 126
Eco24I GRGCYC 2 cut(s) 128, 197
Eco47I GGWCC 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 190
EcoICRI GAGCTC 1 cut(s) 126
EcoO109I RGGNCCY 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 214
EcoT38I GRGCYC 2 cut(s) 128, 197
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
FaeI CATG 1 cut(s) 211
FaiI YATR 1 cut(s) 209
FaqI GGGAC 3 cut(s) 31, 151, 194
FatI CATG 1 cut(s) 207
FauI CCCGC 4 cut(s) 15, 95, 170, 204
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 1 cut(s) 200
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 2 cut(s) 128, 197
Fsp4HI GCNGC 1 cut(s) 200
GlaI GCGC 2 cut(s) 64, 187
GluI GCNGC 1 cut(s) 200
HaeII RGCGCY 2 cut(s) 66, 189
HaeIII GGCC 1 cut(s) 195
HapII CCGG 2 cut(s) 80, 191
HgaI GACGC 1 cut(s) 119
HhaI GCGC 2 cut(s) 65, 188
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 1 cut(s) 211
Hin6I GCGC 2 cut(s) 63, 186
HinP1I GCGC 2 cut(s) 63, 186
HpaII CCGG 2 cut(s) 80, 191
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 1 cut(s) 156
Hpy8I GTNNAC 1 cut(s) 156
Hpy99I CGWCG 2 cut(s) 62, 71
HpyAV CCTTC 1 cut(s) 45
HpyCH4IV ACGT 1 cut(s) 164
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 119, 123, 183, 192
HpyF3I CTNAG 1 cut(s) 118
HpySE526I ACGT 1 cut(s) 164
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 1 cut(s) 211
HspAI GCGC 2 cut(s) 63, 186
KasI GGCGCC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 2 cut(s) 51, 144
LmnI GCTCC 1 cut(s) 27
LpnPI CCDG 6 cut(s) 93, 112, 182, 201, 204, 228
MaeII ACGT 1 cut(s) 164
MalI GATC 2 cut(s) 53, 146
MbiI CCGCTC 1 cut(s) 22
MboI GATC 2 cut(s) 51, 144
MhlI GDGCHC 3 cut(s) 128, 134, 197
Mly113I GGCGCC 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 1 cut(s) 85
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 2 cut(s) 80, 191
MspR9I CCNGG 4 cut(s) 80, 191, 192, 216
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 1 cut(s) 216
MwoI GCNNNNNNNGC 5 cut(s) 89, 119, 123, 183, 192
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 3 cut(s) 80, 191, 192
NdeII GATC 2 cut(s) 51, 144
NlaIII CATG 1 cut(s) 211
NlaIV GGNNCC 3 cut(s) 64, 73, 195
PasI CCCWGGG 1 cut(s) 215
PctI GAATGC 1 cut(s) 92
PkrI GCNGC 1 cut(s) 201
PluTI GGCGCC 1 cut(s) 66
PpuMI RGGWCCY 1 cut(s) 72
Psp124BI GAGCTC 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 3 cut(s) 64, 73, 195
PspOMI GGGCCC 1 cut(s) 193
PspPI GGNCC 3 cut(s) 72, 193, 194
PspPPI RGGWCCY 1 cut(s) 72
RsaI GTAC 4 cut(s) 5, 29, 86, 212
RsaNI GTAC 4 cut(s) 4, 28, 85, 211
SacI GAGCTC 1 cut(s) 128
SatI GCNGC 1 cut(s) 200
Sau3AI GATC 2 cut(s) 51, 144
Sau96I GGNCC 3 cut(s) 72, 193, 194
ScrFI CCNGG 4 cut(s) 80, 191, 192, 216
SduI GDGCHC 3 cut(s) 128, 134, 197
SetI ASST 3 cut(s) 119, 128, 167
SfoI GGCGCC 1 cut(s) 64
SinI GGWCC 1 cut(s) 72
SmaI CCCGGG 1 cut(s) 192
SsiI CCGC 7 cut(s) 8, 14, 20, 102, 177, 197, 200
SspDI GGCGCC 1 cut(s) 62
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 4 cut(s) 78, 189, 190, 214
TaiI ACGT 1 cut(s) 167
TauI GCSGC 1 cut(s) 202
TspGWI ACGGA 1 cut(s) 46
TspMI CCCGGG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 72
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmaI CCCGGG 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.