MD10G1140700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0024223
Physical Location & Seq
Forward (+)
22518880 .. 22520267
1388 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1140700.v1.1.491

Sequence Viewer

Length: 234 bp
ATGTACCCGCCGCCGCCACCGGTCCCATACAACCCTTCGCACAAGTCCCCAATCTCCGTTGGCGCCGTTGGGTCCTCCCCGGCGTACCACGCATTCACCAGCGGGTTGGACGAGAGCTCAGCGAGCTCGTGCCCAATCACACTGATCATCCCGTCCACGCCAATGTCCCTGTTGGGCGGCGACAGCGCCCCGGGCCCGCCGCCGCCCATGTACCCCGGGACTGCGAACGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

78

Amino Acids

7.67

Weight (kDa)

5.2

Isoelectric Point (pI)

85.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024223)

Species Orthologous Gene IDs
malus_domestica MD05G1135400.v1.1 MD10G1140700.v1.1
pyrus_communis pycom05g22970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 62
AciI CCGC 8 cut(s) 8, 11, 14, 102, 177, 197, 200, 203
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 3 cut(s) 5, 86, 212
AgeI ACCGGT 1 cut(s) 19
AluBI AGCT 2 cut(s) 117, 126
AluI AGCT 2 cut(s) 117, 126
Alw21I GWGCWC 2 cut(s) 119, 128
Ama87I CYCGRG 2 cut(s) 190, 215
AoxI GGCC 1 cut(s) 193
ApaI GGGCCC 1 cut(s) 197
AsiGI ACCGGT 1 cut(s) 19
AspLEI GCGC 2 cut(s) 65, 188
AspS9I GGNCC 4 cut(s) 22, 72, 193, 194
AsuC2I CCSGG 5 cut(s) 80, 191, 192, 216, 217
AsuHPI GGTGA 1 cut(s) 88
AvaI CYCGRG 2 cut(s) 190, 215
AvaII GGWCC 2 cut(s) 22, 72
BaeGI GKGCMC 2 cut(s) 134, 197
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 3 cut(s) 119, 128, 197
BauI CACGAG 1 cut(s) 127
Bbv12I GWGCWC 2 cut(s) 119, 128
BceAI ACGGC 1 cut(s) 50
BclI TGATCA 1 cut(s) 144
BcnI CCSGG 5 cut(s) 80, 191, 192, 216, 217
BfoI RGCGCY 2 cut(s) 66, 189
BisI GCNGC 5 cut(s) 11, 14, 178, 200, 203
BlpI GCTNAGC 1 cut(s) 118
BlsI GCNGC 5 cut(s) 12, 15, 179, 201, 204
Bme1390I CCNGG 5 cut(s) 80, 191, 192, 216, 217
Bme18I GGWCC 2 cut(s) 22, 72
BmeT110I CYCGRG 2 cut(s) 190, 215
BmgT120I GGNCC 4 cut(s) 22, 72, 193, 194
BmiI GGNNCC 4 cut(s) 24, 64, 73, 195
BmrFI CCNGG 5 cut(s) 80, 191, 192, 216, 217
Bpu1102I GCTNAGC 1 cut(s) 118
BpuMI CCSGG 5 cut(s) 80, 191, 192, 216, 217
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 5 cut(s) 78, 189, 190, 214, 215
BsaWI WCCGGW 1 cut(s) 19
Bse118I RCCGGY 1 cut(s) 19
BseDI CCNNGG 5 cut(s) 78, 189, 190, 214, 215
BseGI GGATG 1 cut(s) 147
BseMII CTCAG 1 cut(s) 132
BseSI GKGCMC 2 cut(s) 134, 197
BshFI GGCC 1 cut(s) 195
BshNI GGYRCC 1 cut(s) 62
BshTI ACCGGT 1 cut(s) 19
BsiHKAI GWGCWC 2 cut(s) 119, 128
BsiHKCI CYCGRG 2 cut(s) 190, 215
BsiSI CCGG 4 cut(s) 20, 80, 191, 216
BslFI GGGAC 3 cut(s) 8, 31, 151
BsmFI GGGAC 3 cut(s) 8, 31, 151
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 1 cut(s) 195
BsoBI CYCGRG 2 cut(s) 190, 215
Bsp120I GGGCCC 1 cut(s) 193
Bsp1286I GDGCHC 4 cut(s) 119, 128, 134, 197
Bsp143I GATC 1 cut(s) 144
Bsp1720I GCTNAGC 1 cut(s) 118
BspACI CCGC 8 cut(s) 8, 11, 14, 102, 177, 197, 200, 203
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 1 cut(s) 131
BspLI GGNNCC 4 cut(s) 24, 64, 73, 195
BspT107I GGYRCC 1 cut(s) 62
BsrFI RCCGGY 1 cut(s) 19
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 5 cut(s) 78, 189, 190, 214, 215
BssMI GATC 1 cut(s) 144
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 127
Bst2BI CACGAG 1 cut(s) 127
BstACI GRCGYC 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 124, 197
BstDEI CTNAG 1 cut(s) 118
BstF5I GGATG 1 cut(s) 147
BstH2I RGCGCY 2 cut(s) 66, 189
BstHHI GCGC 2 cut(s) 65, 188
BstKTI GATC 1 cut(s) 147
BstMBI GATC 1 cut(s) 144
BstMWI GCNNNNNNNGC 4 cut(s) 89, 123, 183, 192
BstSCI CCNGG 5 cut(s) 78, 189, 190, 214, 215
BstSLI GKGCMC 2 cut(s) 134, 197
BstXI CCANNNNNNTGG 1 cut(s) 106
BsuRI GGCC 1 cut(s) 195
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 140
Cac8I GCNNGC 2 cut(s) 124, 197
CfoI GCGC 2 cut(s) 65, 188
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 4 cut(s) 22, 72, 193, 194
Cfr9I CCCGGG 2 cut(s) 190, 215
Csp6I GTAC 3 cut(s) 4, 85, 211
CspAI ACCGGT 1 cut(s) 19
CviAII CATG 1 cut(s) 208
CviJI RGCY 3 cut(s) 117, 126, 195
CviKI_1 RGCY 3 cut(s) 117, 126, 195
CviQI GTAC 3 cut(s) 4, 85, 211
DdeI CTNAG 1 cut(s) 118
DinI GGCGCC 1 cut(s) 64
DpnI GATC 1 cut(s) 146
DpnII GATC 1 cut(s) 144
Ecl136II GAGCTC 2 cut(s) 117, 126
Eco24I GRGCYC 3 cut(s) 119, 128, 197
Eco47I GGWCC 2 cut(s) 22, 72
Eco53kI GAGCTC 2 cut(s) 117, 126
Eco88I CYCGRG 2 cut(s) 190, 215
EcoICRI GAGCTC 2 cut(s) 117, 126
EcoO109I RGGNCCY 1 cut(s) 72
EcoT38I GRGCYC 3 cut(s) 119, 128, 197
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
FaeI CATG 1 cut(s) 211
FaiI YATR 2 cut(s) 28, 209
FaqI GGGAC 3 cut(s) 8, 31, 151
FatI CATG 1 cut(s) 207
FauI CCCGC 3 cut(s) 15, 95, 204
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 5 cut(s) 11, 14, 178, 200, 203
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 3 cut(s) 119, 128, 197
Fsp4HI GCNGC 5 cut(s) 11, 14, 178, 200, 203
GlaI GCGC 2 cut(s) 64, 187
GluI GCNGC 5 cut(s) 11, 14, 178, 200, 203
HaeII RGCGCY 2 cut(s) 66, 189
HaeIII GGCC 1 cut(s) 195
HapII CCGG 4 cut(s) 20, 80, 191, 216
HhaI GCGC 2 cut(s) 65, 188
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 1 cut(s) 211
Hin6I GCGC 2 cut(s) 63, 186
HinP1I GCGC 2 cut(s) 63, 186
HpaII CCGG 4 cut(s) 20, 80, 191, 216
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 1 cut(s) 156
Hpy8I GTNNAC 1 cut(s) 156
HpyAV CCTTC 1 cut(s) 45
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 123, 183, 192
HpyF3I CTNAG 1 cut(s) 118
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 1 cut(s) 211
HspAI GCGC 2 cut(s) 63, 186
KasI GGCGCC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 1 cut(s) 144
LpnPI CCDG 6 cut(s) 33, 93, 112, 182, 204, 229
MalI GATC 1 cut(s) 146
MboI GATC 1 cut(s) 144
MhlI GDGCHC 4 cut(s) 119, 128, 134, 197
Mly113I GGCGCC 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 1 cut(s) 85
MslI CAYNNNNRTG 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 4 cut(s) 20, 80, 191, 216
MspR9I CCNGG 5 cut(s) 80, 191, 192, 216, 217
Mva1269I GAATGC 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 89, 123, 183, 192
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 5 cut(s) 80, 191, 192, 216, 217
NdeII GATC 1 cut(s) 144
NlaIII CATG 1 cut(s) 211
NlaIV GGNNCC 4 cut(s) 24, 64, 73, 195
PctI GAATGC 1 cut(s) 92
PinAI ACCGGT 1 cut(s) 19
PkrI GCNGC 5 cut(s) 12, 15, 179, 201, 204
PluTI GGCGCC 1 cut(s) 66
PpuMI RGGWCCY 1 cut(s) 72
Psp124BI GAGCTC 2 cut(s) 119, 128
Psp5II RGGWCCY 1 cut(s) 72
PspN4I GGNNCC 4 cut(s) 24, 64, 73, 195
PspOMI GGGCCC 1 cut(s) 193
PspPI GGNCC 4 cut(s) 22, 72, 193, 194
PspPPI RGGWCCY 1 cut(s) 72
RsaI GTAC 3 cut(s) 5, 86, 212
RsaNI GTAC 3 cut(s) 4, 85, 211
RseI CAYNNNNRTG 1 cut(s) 161
SacI GAGCTC 2 cut(s) 119, 128
SatI GCNGC 5 cut(s) 11, 14, 178, 200, 203
Sau3AI GATC 1 cut(s) 144
Sau96I GGNCC 4 cut(s) 22, 72, 193, 194
ScrFI CCNGG 5 cut(s) 80, 191, 192, 216, 217
SduI GDGCHC 4 cut(s) 119, 128, 134, 197
SetI ASST 2 cut(s) 119, 128
SfoI GGCGCC 1 cut(s) 64
SinI GGWCC 2 cut(s) 22, 72
SmaI CCCGGG 2 cut(s) 192, 217
SmiMI CAYNNNNRTG 1 cut(s) 161
SsiI CCGC 8 cut(s) 8, 11, 14, 102, 177, 197, 200, 203
SspDI GGCGCC 1 cut(s) 62
SstI GAGCTC 2 cut(s) 119, 128
StyD4I CCNGG 5 cut(s) 78, 189, 190, 214, 215
TauI GCSGC 5 cut(s) 13, 16, 180, 202, 205
TscAI CASTG 1 cut(s) 147
TspGWI ACGGA 1 cut(s) 46
TspMI CCCGGG 2 cut(s) 190, 215
TspRI CASTG 1 cut(s) 147
VpaK11BI GGWCC 2 cut(s) 22, 72
XmaI CCCGGG 2 cut(s) 190, 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.