MD05G1148200.v1.1

Toll - interleukin 1 - resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024457
Physical Location & Seq
Reverse (-)
27952409 .. 27953751
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1148200.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGCACGATATGCTACAAGAAATGGGAAAGACAATTGTTCAAGAAATGGGGATACTTGTCAACTTACAAGTGATTGATCTCCGGGGCTGTTCAAATCTAACTGAAGTTCCAAATCTCTCTAGAAGTCTAAAAATTGTGGAGATAAATCTCGGGCACTGTCGAAGTTTGGTTGAAATTCCTTCGTATTTTCAACATCTTGACAAGCTTACTCATCTTGATCTTGGATTCTGCTACAGTCTCAAGTATCTTCCAGAGATGCCAGGAAATATTCAATACTTAAATTTAGAAGGCAGTGGTATAAAGGAGTTGCCTGAATCAGTTTGGTGTAACGAAAATATTTCTTACTTGAATTTAAGGCAATGCGAAGACCTTAAGAAACTTCCAAGCAGCAGGTGTAAGTTGAAAAATCTCGATCTCCATAGGTGCTCTAAATTTGAAAACTTTCCAGAGATTTTGGAGCCAATGGAACATTTGAAGTCCTTAAGTTTAAGTCAAACAGCAGTTACAGAGCTACCCTCATCAATCTGTAAGTTGAAAAATCTTGAGAAACTCGATCTCTGTTCGTGCTCTAAATTTGAAAACTTCCCAGAGATTTTGGAGCCAATGGAACATTTGAAGTCCTTAAGTTTAATTCAAACACCAGTTAGAGAGCTACCCTCATCAATCTGTAAGTTGAAATATCTTGAGAGTCTTTATCTCACCAATTGCTATAGATTTTCCAAATTCCCTGAAATCTTGAAGCCAATGGAACATTTGGTGTCTCTTTGTTTGGAAGAGACAGCTGTCGAAATGCTTCCTTCGTCTATTGGAAATCTAAATGGGCTTCAAGATTTAAACCTTAGTCGCTGCTATCTACTTAAGGATGTCCCAACAAGTATCTACAGTTTAACCAATCTTAAACGTCTCAACTTTAATGACTGTCGGAGACTTGAAAAATTGCCTTCCTGCTCTGTTGGTTTTTTCTCTTTAGAAGAACTAGATCTCAGCAACAGCGGTATATTGGAAATACCAGCAAGCATCAAACAAGCTTCTCGGTTCTCCCAGTGCTATGCAATGTTAAAGCTTCAAACAGCACGTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

361

Amino Acids

41.18

Weight (kDa)

6.28

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 89 - 162 6.1e-07 Leucine-rich repeat region
LRR_14 PF23598 137 - 196 3.9e-06 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 150 - 211 1.7e-06 R13L1/DRL21 LRRs
LRR_14 PF23598 202 - 259 1.7e-06 Leucine-rich repeat region
LRR_13 PF23286 226 - 311 4.1e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 250 - 330 2.9e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024457)

Species Orthologous Gene IDs
malus_domestica MD05G1148200.v1.1
prunus_persica Prupe.4G248700_v2.0.a1
pyrus_communis pycom05g13340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 381
Acc36I ACCTGC 1 cut(s) 381
AciI CCGC 1 cut(s) 993
AcsI RAATTY 6 cut(s) 174, 280, 349, 431, 572, 722
AcuI CTGAAG 1 cut(s) 123
AflII CTTAAG 4 cut(s) 371, 481, 622, 857
AjiI CACGTC 1 cut(s) 1076
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 6 cut(s) 205, 511, 652, 782, 1028, 1063
AluI AGCT 6 cut(s) 205, 511, 652, 782, 1028, 1063
Alw21I GWGCWC 2 cut(s) 428, 569
Alw26I GTCTC 5 cut(s) 242, 765, 770, 908, 919
Ama87I CYCGRG 1 cut(s) 149
ApeKI GCWGC 2 cut(s) 387, 846
ApoI RAATTY 6 cut(s) 174, 280, 349, 431, 572, 722
ArsI GACNNNNNNTTYG 1 cut(s) 1060
Asp700I GAANNNNTTC 2 cut(s) 441, 792
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 691
AvaI CYCGRG 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 372
Bbv12I GWGCWC 2 cut(s) 428, 569
BbvI GCAGC 2 cut(s) 399, 833
BciT130I CCWGG 1 cut(s) 261
BciVI GTATCC 1 cut(s) 45
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 5 cut(s) 242, 765, 770, 908, 919
BfaI CTAG 2 cut(s) 120, 977
BfmI CTRYAG 3 cut(s) 232, 709, 880
BfrI CTTAAG 4 cut(s) 371, 481, 622, 857
BfuAI ACCTGC 1 cut(s) 381
BfuI GTATCC 1 cut(s) 45
BglII AGATCT 1 cut(s) 979
BisI GCNGC 2 cut(s) 388, 847
BlsI GCNGC 2 cut(s) 389, 848
Bme1390I CCNGG 2 cut(s) 83, 261
BmeT110I CYCGRG 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 1076
BmiI GGNNCC 2 cut(s) 459, 600
BmrFI CCNGG 2 cut(s) 83, 261
BmrI ACTGGG 1 cut(s) 1036
BmsI GCATC 2 cut(s) 246, 1026
BmuI ACTGGG 1 cut(s) 1036
BoxI GACNNNNGTC 1 cut(s) 782
BpiI GAAGAC 1 cut(s) 372
BplI GAGNNNNNCTC 4 cut(s) 500, 532, 641, 673
BpuEI CTTGAG 3 cut(s) 224, 563, 704
BpuMI CCSGG 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 82
Bse1I ACTGG 2 cut(s) 641, 1042
Bse3DI GCAATG 2 cut(s) 365, 1059
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 868
BseMI GCAATG 2 cut(s) 365, 1059
BseMII CTCAG 1 cut(s) 997
BseNI ACTGG 2 cut(s) 641, 1042
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 2 cut(s) 399, 833
BsiHKAI GWGCWC 2 cut(s) 428, 569
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 1 cut(s) 82
BslFI GGGAC 1 cut(s) 851
BsmAI GTCTC 5 cut(s) 242, 765, 770, 908, 919
BsmBI CGTCTC 1 cut(s) 908
BsmFI GGGAC 1 cut(s) 851
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 3 cut(s) 156, 428, 569
Bsp143I GATC 5 cut(s) 76, 217, 412, 553, 979
BspACI CCGC 1 cut(s) 993
BspCNI CTCAG 1 cut(s) 996
BspLI GGNNCC 2 cut(s) 459, 600
BspMI ACCTGC 1 cut(s) 381
BspTI CTTAAG 4 cut(s) 371, 481, 622, 857
BsrDI GCAATG 2 cut(s) 365, 1059
BsrI ACTGG 2 cut(s) 641, 1042
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 5 cut(s) 76, 217, 412, 553, 979
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 4 cut(s) 158, 236, 884, 920
Bst6I CTCTTC 1 cut(s) 768
BstAFI CTTAAG 4 cut(s) 371, 481, 622, 857
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 1015
BstDEI CTNAG 2 cut(s) 839, 983
BstF5I GGATG 1 cut(s) 868
BstKTI GATC 5 cut(s) 79, 220, 415, 556, 982
BstMAI GTCTC 5 cut(s) 242, 765, 770, 908, 919
BstMBI GATC 5 cut(s) 76, 217, 412, 553, 979
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 261
BstPAI GACNNNNGTC 1 cut(s) 782
BstSCI CCNGG 2 cut(s) 81, 259
BstSFI CTRYAG 3 cut(s) 232, 709, 880
BstSLI GKGCMC 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 399, 833
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 1 cut(s) 979
BstYI RGATCY 1 cut(s) 979
BsuI GTATCC 1 cut(s) 45
BtrI CACGTC 1 cut(s) 1076
BtsCI GGATG 1 cut(s) 868
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 3 cut(s) 154, 298, 1049
BveI ACCTGC 1 cut(s) 381
Cac8I GCNNGC 1 cut(s) 1015
DdeI CTNAG 2 cut(s) 839, 983
DpnI GATC 5 cut(s) 78, 219, 414, 555, 981
DpnII GATC 5 cut(s) 76, 217, 412, 553, 979
DraI TTTAAA 1 cut(s) 834
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco57I CTGAAG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 259
Esp3I CGTCTC 1 cut(s) 908
FaiI YATR 6 cut(s) 11, 299, 420, 711, 998, 1050
FaqI GGGAC 1 cut(s) 851
Fnu4HI GCNGC 2 cut(s) 388, 847
FokI GGATG 1 cut(s) 875
Fsp4HI GCNGC 2 cut(s) 388, 847
FspBI CTAG 2 cut(s) 120, 977
GluI GCNGC 2 cut(s) 388, 847
HapII CCGG 1 cut(s) 82
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HindIII AAGCTT 3 cut(s) 203, 1026, 1061
HinfI GANTC 3 cut(s) 225, 314, 688
HpaII CCGG 1 cut(s) 82
HphI GGTGA 1 cut(s) 691
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 1 cut(s) 924
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 1 cut(s) 1080
HpyAV CCTTC 4 cut(s) 189, 281, 807, 951
HpyCH4III ACNGT 4 cut(s) 158, 236, 884, 920
HpyCH4IV ACGT 2 cut(s) 901, 1075
HpyCH4V TGCA 2 cut(s) 4, 1052
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 2 cut(s) 839, 983
HpySE526I ACGT 2 cut(s) 901, 1075
Kzo9I GATC 5 cut(s) 76, 217, 412, 553, 979
LmnI GCTCC 2 cut(s) 457, 598
Lsp1109I GCAGC 2 cut(s) 399, 833
LweI GCATC 2 cut(s) 246, 1026
MaeI CTAG 2 cut(s) 120, 977
MaeII ACGT 2 cut(s) 901, 1075
MaeIII GTNAC 2 cut(s) 326, 502
MalI GATC 5 cut(s) 78, 219, 414, 555, 981
MboI GATC 5 cut(s) 76, 217, 412, 553, 979
MboII GAAGA 4 cut(s) 239, 377, 785, 983
MfeI CAATTG 2 cut(s) 33, 703
MflI RGATCY 1 cut(s) 979
MhlI GDGCHC 3 cut(s) 156, 428, 569
MlyI GAGTC 1 cut(s) 697
MmeI TCCRAC 1 cut(s) 902
MnlI CCTC 2 cut(s) 526, 667
MroXI GAANNNNTTC 2 cut(s) 441, 792
MspA1I CMGCKG 2 cut(s) 782, 993
MspCI CTTAAG 4 cut(s) 371, 481, 622, 857
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 2 cut(s) 83, 261
MunI CAATTG 2 cut(s) 33, 703
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 1 cut(s) 83
NdeII GATC 5 cut(s) 76, 217, 412, 553, 979
NlaIV GGNNCC 2 cut(s) 459, 600
PaqCI CACCTGC 1 cut(s) 381
PdmI GAANNNNTTC 2 cut(s) 441, 792
PfeI GAWTC 2 cut(s) 225, 314
PkrI GCNGC 2 cut(s) 389, 848
PleI GAGTC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 696
PshAI GACNNNNGTC 1 cut(s) 782
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 459, 600
PsuI RGATCY 1 cut(s) 979
PvuII CAGCTG 1 cut(s) 782
SatI GCNGC 2 cut(s) 388, 847
Sau3AI GATC 5 cut(s) 76, 217, 412, 553, 979
SchI GAGTC 1 cut(s) 697
ScrFI CCNGG 2 cut(s) 83, 261
SduI GDGCHC 3 cut(s) 156, 428, 569
SfaNI GCATC 2 cut(s) 246, 1026
SfcI CTRYAG 3 cut(s) 232, 709, 880
SmlI CTYRAG 7 cut(s) 239, 371, 481, 542, 622, 683, 857
SmoI CTYRAG 7 cut(s) 239, 371, 481, 542, 622, 683, 857
SsiI CCGC 1 cut(s) 993
SspI AATATT 2 cut(s) 268, 337
SspMI CTAG 2 cut(s) 120, 977
StyD4I CCNGG 2 cut(s) 81, 259
TaaI ACNGT 4 cut(s) 158, 236, 884, 920
TaiI ACGT 2 cut(s) 904, 1078
TaqI TCGA 4 cut(s) 160, 411, 552, 786
TfiI GAWTC 2 cut(s) 225, 314
TscAI CASTG 3 cut(s) 161, 298, 1049
TseI GCWGC 2 cut(s) 387, 846
TspRI CASTG 3 cut(s) 161, 298, 1049
Vha464I CTTAAG 4 cut(s) 371, 481, 622, 857
XapI RAATTY 6 cut(s) 174, 280, 349, 431, 572, 722
XbaI TCTAGA 1 cut(s) 119
XmnI GAANNNNTTC 2 cut(s) 441, 792
XspI CTAG 2 cut(s) 120, 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.