MD05G1220000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024465
Physical Location & Seq
Forward (+)
35291830 .. 35293112
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1220000.v1.1.491

Sequence Viewer

Length: 261 bp
ATGTGCAATGCTGCTGCTTCTTCCATGGCTGCTCTCAAGCAACCAACCATTAGAGTTGTGGCAATTGATCGCATGTGCACGGGCAAACAACAAGGTTGTCATTGGCCCAGCTACTGTTGGAGATTGGTGACACTGCTGGAACAATTGATAATATTATTCACTGCAAGCTATACAGGACTGGATCTGTTGGTTTTGTCTCCAAATCTGTATAATGGACAGCCTCCCGTGGTTTACAAGCTTTTAAACCACCAAAAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

87

Amino Acids

9.66

Weight (kDa)

9.3

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024465)

Species Orthologous Gene IDs
malus_domestica MD05G1220000.v1.1
pyrus_communis pycom07g27250 pycom14g01700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 189
AluBI AGCT 3 cut(s) 111, 168, 238
AluI AGCT 3 cut(s) 111, 168, 238
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 1 cut(s) 201
Alw44I GTGCAC 1 cut(s) 76
AlwI GGATC 1 cut(s) 189
AlwNI CAGNNNCTG 1 cut(s) 114
AoxI GGCC 1 cut(s) 104
ApaLI GTGCAC 1 cut(s) 76
ApeKI GCWGC 3 cut(s) 11, 14, 29
AspS9I GGNCC 1 cut(s) 105
AsuHPI GGTGA 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 201
BisI GCNGC 3 cut(s) 12, 15, 30
BlsI GCNGC 3 cut(s) 13, 16, 31
BmgT120I GGNCC 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 24, 225
Bse1I ACTGG 1 cut(s) 183
Bse3DI GCAATG 1 cut(s) 13
BseDI CCNNGG 2 cut(s) 24, 225
BseMI GCAATG 1 cut(s) 13
BseNI ACTGG 1 cut(s) 183
BseSI GKGCMC 1 cut(s) 80
BseXI GCAGC 1 cut(s) 16
BseYI CCCAGC 1 cut(s) 107
BshFI GGCC 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 80
BsmAI GTCTC 1 cut(s) 201
BsnI GGCC 1 cut(s) 106
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 2 cut(s) 67, 181
Bsp19I CCATGG 1 cut(s) 24
BspANI GGCC 1 cut(s) 106
BspPI GGATC 1 cut(s) 189
BsrDI GCAATG 1 cut(s) 13
BsrI ACTGG 1 cut(s) 183
BssECI CCNNGG 2 cut(s) 24, 225
BssMI GATC 2 cut(s) 67, 181
BssT1I CCWWGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 116
BstC8I GCNNGC 1 cut(s) 166
BstDSI CCRYGG 2 cut(s) 24, 225
BstKTI GATC 2 cut(s) 70, 184
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 2 cut(s) 67, 181
BstNSI RCATGY 1 cut(s) 76
BstSLI GKGCMC 1 cut(s) 80
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 1 cut(s) 106
BtgI CCRYGG 2 cut(s) 24, 225
BtsI GCAGTG 2 cut(s) 131, 159
BtsIMutI CAGTG 2 cut(s) 131, 159
Cac8I GCNNGC 1 cut(s) 166
CaiI CAGNNNCTG 1 cut(s) 114
Cfr13I GGNCC 1 cut(s) 105
CviAII CATG 2 cut(s) 25, 73
CviJI RGCY 6 cut(s) 29, 106, 111, 168, 220, 238
CviKI_1 RGCY 6 cut(s) 29, 106, 111, 168, 220, 238
DpnI GATC 2 cut(s) 69, 183
DpnII GATC 2 cut(s) 67, 181
DraI TTTAAA 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaeI CATG 2 cut(s) 28, 76
FaiI YATR 4 cut(s) 26, 74, 171, 210
FatI CATG 2 cut(s) 24, 72
Fnu4HI GCNGC 3 cut(s) 12, 15, 30
Fsp4HI GCNGC 3 cut(s) 12, 15, 30
GluI GCNGC 3 cut(s) 12, 15, 30
GsaI CCCAGC 1 cut(s) 111
HaeIII GGCC 1 cut(s) 106
Hin1II CATG 2 cut(s) 28, 76
HindIII AAGCTT 1 cut(s) 236
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 2 cut(s) 78, 232
Hpy8I GTNNAC 2 cut(s) 78, 232
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4V TGCA 3 cut(s) 6, 78, 164
Hsp92II CATG 2 cut(s) 28, 76
Kzo9I GATC 2 cut(s) 67, 181
LpnPI CCDG 4 cut(s) 121, 122, 159, 164
Lsp1109I GCAGC 1 cut(s) 16
MaeIII GTNAC 1 cut(s) 127
MalI GATC 2 cut(s) 69, 183
MboI GATC 2 cut(s) 67, 181
MboII GAAGA 1 cut(s) 12
MfeI CAATTG 2 cut(s) 63, 143
MflI RGATCY 1 cut(s) 181
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 2 cut(s) 63, 143
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 1 cut(s) 231
MseI TTAA 1 cut(s) 242
MunI CAATTG 2 cut(s) 63, 143
NcoI CCATGG 1 cut(s) 24
NdeII GATC 2 cut(s) 67, 181
NlaIII CATG 2 cut(s) 28, 76
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 76
PkrI GCNGC 3 cut(s) 13, 16, 31
PspFI CCCAGC 1 cut(s) 107
PspPI GGNCC 1 cut(s) 105
PstNI CAGNNNCTG 1 cut(s) 114
PsuI RGATCY 1 cut(s) 181
SaqAI TTAA 1 cut(s) 242
SatI GCNGC 3 cut(s) 12, 15, 30
Sau3AI GATC 2 cut(s) 67, 181
Sau96I GGNCC 1 cut(s) 105
SduI GDGCHC 1 cut(s) 80
SetI ASST 4 cut(s) 97, 113, 170, 240
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 2 cut(s) 63, 143
SspI AATATT 1 cut(s) 153
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 116
TasI AATT 2 cut(s) 63, 143
Tru1I TTAA 1 cut(s) 242
Tru9I TTAA 1 cut(s) 242
TscAI CASTG 2 cut(s) 138, 166
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 3 cut(s) 11, 14, 29
Tsp45I GTSAC 1 cut(s) 127
TspRI CASTG 2 cut(s) 138, 166
VneI GTGCAC 1 cut(s) 76
XceI RCATGY 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.