MD05G1235100.v1.1

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0028612
Physical Location & Seq
Forward (+)
36716906 .. 36721027
4122 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1235100.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGCACTATAAGCCTAGCTTAGGCTTAAGGCCTATCCTCACTCTTTCACCTTTTAATAAATATTTAGAGGAGCAAGTTAAAGGCTATAGTGATGGAGATGAAGAGAAATGGGGTGCTTTTCAGCCTTTCCAAAGGAAAAAAAAAAGCATTGGAAAGACAAATGGACCGGTGGCTAAAGAACCTTCTTCGGCTCCGGCTACTAGTTCCACCACGGACACCGTGAGTGGAGACAGTGGTGGGAACAACAAGAACAAAGAGAAAGATGGACAGGGAAACGAAGGCGAAACTGGTCACCGGAGTTGCACCGCCGGTTCTTGCATGCTCTTCTCTTCAACAACTTGGTGGTTCACATGGTATGCAGTTGAGATTAATTTAGTGGGATATGTGAACCAACTTGCGGGCAACGAAAGGATTGCAATATATGCATCTAATGTGGCTAACTTGGTGCTTTTCGTGGCAAAAGTTTATGCTTCAGTTGAAAGCAGATCTTTGGCAGCTTCATTCTTTGGTTCATTTAATCAGTATCGGTACCCGATTCGTAAAGCCCGGATGCAACCTGTGGGAATTATTGTTTTTGCATCTGTAATGGCAACTCCTGGACCTCAAATATTGTTCGAATCTGGCCGGCAACTTCTCATGAAGACTCAACCTGAAATGGATCCAGTGAAAGAAAAATGGATGATAGGGATAATAGTGTCTGCAACAGTTGTGAAGTTTGTGTTGGTGGTGTATTGTCGTAGATTCAAGAACGAAATTGTTCGAGCTTACGCTCAAGATCATCTGTTTGATGTCATCACTAATGGAATTGGTCTTGCAGCTGCAGTTTTAGCAATCAGGTTTTACTGGTGGCTTGATCCTGTTGGAGCTATCATTTACTTGGCAAAATTGACATACCTGATATGGAACGATGACAAGGAGATCAAGCACATTGACACGGTGAGAGCATACACTTTTGGCTGCAACTATTTTGTGGAGGCTGACATTGTCTTGCCCGGAGACATGTCTCTCAGCCACGCACATAACATCGGAGAGACGCTTCAGGAAAAGATCGAAAACGTTCCTGAGGTGGAACGAGCTTTCGTTCATGTGAGACTACTCACAGGCCTGAGCACAACAATGCAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

376

Amino Acids

41.85

Weight (kDa)

8.48

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 174 - 296 3.5e-10 Cation efflux transmembrane domain
ZT_dimer PF16916 305 - 363 3e-07 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028612)

Species Orthologous Gene IDs
malus_domestica MD01G1189800.v1.1 MD05G1235100.v1.1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 1 cut(s) 528
AciI CCGC 2 cut(s) 306, 398
AclI AACGTT 1 cut(s) 1056
AclWI GGATC 3 cut(s) 653, 666, 848
AcoI YGGCCR 1 cut(s) 622
AcuI CTGAAG 2 cut(s) 456, 1022
AfaI GTAC 1 cut(s) 530
AfiI CCNNNNNNNGG 2 cut(s) 20, 397
AflII CTTAAG 1 cut(s) 25
AflIII ACRYGT 1 cut(s) 999
AgeI ACCGGT 1 cut(s) 166
AgsI TTSAA 3 cut(s) 333, 479, 745
AhlI ACTAGT 1 cut(s) 200
AjnI CCWGG 1 cut(s) 595
AjuI GAANNNNNNNTTGG 2 cut(s) 704, 736
AluBI AGCT 6 cut(s) 18, 497, 764, 818, 866, 1076
AluI AGCT 6 cut(s) 18, 497, 764, 818, 866, 1076
Alw21I GWGCWC 1 cut(s) 1112
Alw26I GTCTC 5 cut(s) 222, 990, 1008, 1025, 1084
AlwI GGATC 3 cut(s) 653, 666, 848
AoxI GGCC 3 cut(s) 29, 622, 1102
ApeKI GCWGC 4 cut(s) 494, 815, 818, 957
AseI ATTAAT 1 cut(s) 369
AsiGI ACCGGT 1 cut(s) 166
Asp700I GAANNNNTTC 2 cut(s) 756, 1056
Asp718I GGTACC 1 cut(s) 528
AspS9I GGNCC 2 cut(s) 164, 599
AsuC2I CCSGG 2 cut(s) 547, 993
AsuHPI GGTGA 3 cut(s) 39, 284, 949
AsuII TTCGAA 1 cut(s) 615
AvaII GGWCC 2 cut(s) 164, 599
AxyI CCTNAGG 1 cut(s) 1062
BamHI GGATCC 1 cut(s) 658
BanI GGYRCC 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 647
Bbv12I GWGCWC 1 cut(s) 1112
BbvI GCAGC 4 cut(s) 506, 805, 827, 944
BccI CCATC 2 cut(s) 86, 257
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 1 cut(s) 597
BcnI CCSGG 2 cut(s) 547, 993
BcoDI GTCTC 5 cut(s) 222, 990, 1008, 1025, 1084
BcuI ACTAGT 1 cut(s) 200
BfaI CTAG 2 cut(s) 15, 201
BfmI CTRYAG 2 cut(s) 85, 819
BfrI CTTAAG 1 cut(s) 25
BglII AGATCT 1 cut(s) 485
BisI GCNGC 4 cut(s) 495, 816, 819, 958
BlsI GCNGC 4 cut(s) 496, 817, 820, 959
Bme1390I CCNGG 3 cut(s) 547, 597, 993
Bme18I GGWCC 2 cut(s) 164, 599
BmgT120I GGNCC 2 cut(s) 164, 599
BmiI GGNNCC 3 cut(s) 192, 530, 660
BmrFI CCNGG 3 cut(s) 547, 597, 993
BmsI GCATC 3 cut(s) 434, 540, 587
BpiI GAAGAC 1 cut(s) 647
Bpu10I CCTNAGC 2 cut(s) 19, 1106
Bpu14I TTCGAA 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 756
BpuMI CCSGG 2 cut(s) 547, 993
BsaJI CCNNGG 1 cut(s) 210
BsaWI WCCGGW 2 cut(s) 166, 294
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 397
Bse118I RCCGGY 3 cut(s) 166, 308, 624
Bse1I ACTGG 3 cut(s) 292, 662, 848
Bse21I CCTNAGG 1 cut(s) 1062
BseBI CCWGG 1 cut(s) 597
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 2 cut(s) 555, 684
BseLI CCNNNNNNNGG 2 cut(s) 20, 397
BseMII CTCAG 3 cut(s) 1021, 1053, 1097
BseNI ACTGG 3 cut(s) 292, 662, 848
BseRI GAGGAG 1 cut(s) 83
BseXI GCAGC 4 cut(s) 506, 805, 827, 944
BshFI GGCC 3 cut(s) 31, 624, 1104
BshNI GGYRCC 1 cut(s) 528
BshTI ACCGGT 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 1112
BsiSI CCGG 7 cut(s) 167, 194, 295, 309, 547, 625, 993
BslI CCNNNNNNNGG 2 cut(s) 20, 397
BsmAI GTCTC 5 cut(s) 222, 990, 1008, 1025, 1084
BsmBI CGTCTC 1 cut(s) 1025
BsnI GGCC 3 cut(s) 31, 624, 1104
Bsp119I TTCGAA 1 cut(s) 615
Bsp1286I GDGCHC 1 cut(s) 1112
Bsp143I GATC 6 cut(s) 485, 658, 775, 853, 918, 1047
BspACI CCGC 2 cut(s) 306, 398
BspANI GGCC 3 cut(s) 31, 624, 1104
BspCNI CTCAG 3 cut(s) 1020, 1054, 1098
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 3 cut(s) 192, 530, 660
BspMAI CTGCAG 1 cut(s) 823
BspPI GGATC 3 cut(s) 653, 666, 848
BspQI GCTCTTC 1 cut(s) 329
BspT104I TTCGAA 1 cut(s) 615
BspT107I GGYRCC 1 cut(s) 528
BspTI CTTAAG 1 cut(s) 25
BsrFI RCCGGY 3 cut(s) 166, 308, 624
BsrI ACTGG 3 cut(s) 292, 662, 848
BssAI RCCGGY 3 cut(s) 166, 308, 624
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 6 cut(s) 485, 658, 775, 853, 918, 1047
Bst2UI CCWGG 1 cut(s) 597
Bst4CI ACNGT 4 cut(s) 220, 233, 706, 937
Bst6I CTCTTC 3 cut(s) 96, 329, 334
BstAFI CTTAAG 1 cut(s) 25
BstAPI GCANNNNNTGC 1 cut(s) 422
BstBI TTCGAA 1 cut(s) 615
BstC8I GCNNGC 3 cut(s) 320, 400, 626
BstDEI CTNAG 4 cut(s) 19, 1007, 1062, 1106
BstDSI CCRYGG 1 cut(s) 210
BstEII GGTNACC 1 cut(s) 290
BstENI CCTNNNNNAGG 1 cut(s) 18
BstF5I GGATG 2 cut(s) 555, 684
BstKTI GATC 6 cut(s) 488, 661, 778, 856, 921, 1050
BstMAI GTCTC 5 cut(s) 222, 990, 1008, 1025, 1084
BstMBI GATC 6 cut(s) 485, 658, 775, 853, 918, 1047
BstMWI GCNNNNNNNGC 3 cut(s) 10, 422, 827
BstNI CCWGG 1 cut(s) 597
BstNSI RCATGY 2 cut(s) 322, 1003
BstPI GGTNACC 1 cut(s) 290
BstSCI CCNGG 3 cut(s) 545, 595, 991
BstSFI CTRYAG 2 cut(s) 85, 819
BstV1I GCAGC 4 cut(s) 506, 805, 827, 944
BstV2I GAAGAC 1 cut(s) 647
BstX2I RGATCY 2 cut(s) 485, 658
BstYI RGATCY 2 cut(s) 485, 658
Bsu36I CCTNAGG 1 cut(s) 1062
BsuRI GGCC 3 cut(s) 31, 624, 1104
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 2 cut(s) 555, 684
BtsIMutI CAGTG 2 cut(s) 238, 669
Cac8I GCNNGC 3 cut(s) 320, 400, 626
CciI TCATGA 1 cut(s) 636
Cfr10I RCCGGY 3 cut(s) 166, 308, 624
Cfr13I GGNCC 2 cut(s) 164, 599
CseI GACGC 1 cut(s) 1042
Csp6I GTAC 1 cut(s) 529
CspAI ACCGGT 1 cut(s) 166
CviAII CATG 5 cut(s) 319, 351, 637, 1000, 1085
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 4 cut(s) 19, 1007, 1062, 1106
DpnI GATC 6 cut(s) 487, 660, 777, 855, 920, 1049
DpnII GATC 6 cut(s) 485, 658, 775, 853, 918, 1047
EaeI YGGCCR 1 cut(s) 622
Eam1104I CTCTTC 3 cut(s) 96, 329, 334
EarI CTCTTC 3 cut(s) 96, 329, 334
Eco147I AGGCCT 2 cut(s) 31, 1104
Eco47I GGWCC 2 cut(s) 164, 599
Eco57I CTGAAG 2 cut(s) 456, 1022
Eco81I CCTNAGG 1 cut(s) 1062
Eco91I GGTNACC 1 cut(s) 290
EcoNI CCTNNNNNAGG 1 cut(s) 18
EcoO65I GGTNACC 1 cut(s) 290
EcoRII CCWGG 1 cut(s) 595
EcoT22I ATGCAT 1 cut(s) 427
Esp3I CGTCTC 1 cut(s) 1025
FaeI CATG 5 cut(s) 322, 354, 640, 1003, 1088
FalI AAGNNNNNCTT 3 cut(s) 34, 472, 504
FatI CATG 5 cut(s) 318, 350, 636, 999, 1084
FauI CCCGC 1 cut(s) 391
Fnu4HI GCNGC 4 cut(s) 495, 816, 819, 958
FokI GGATG 2 cut(s) 562, 691
Fsp4HI GCNGC 4 cut(s) 495, 816, 819, 958
FspBI CTAG 2 cut(s) 15, 201
GluI GCNGC 4 cut(s) 495, 816, 819, 958
HaeIII GGCC 3 cut(s) 31, 624, 1104
HapII CCGG 7 cut(s) 167, 194, 295, 309, 547, 625, 993
HgaI GACGC 1 cut(s) 1042
Hin1II CATG 5 cut(s) 322, 354, 640, 1003, 1088
HinfI GANTC 4 cut(s) 535, 617, 643, 741
HpaII CCGG 7 cut(s) 167, 194, 295, 309, 547, 625, 993
HphI GGTGA 3 cut(s) 39, 284, 949
Hpy166II GTNNAC 2 cut(s) 348, 388
Hpy188I TCNGA 1 cut(s) 1028
Hpy188III TCNNGA 5 cut(s) 637, 745, 773, 1040, 1061
Hpy8I GTNNAC 2 cut(s) 348, 388
HpyAV CCTTC 2 cut(s) 192, 272
HpyCH4III ACNGT 4 cut(s) 220, 233, 706, 937
HpyCH4IV ACGT 1 cut(s) 1056
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 422, 827
HpyF3I CTNAG 4 cut(s) 19, 1007, 1062, 1106
HpySE526I ACGT 1 cut(s) 1056
Hsp92II CATG 5 cut(s) 322, 354, 640, 1003, 1088
KpnI GGTACC 1 cut(s) 532
KroI GCCGGC 1 cut(s) 624
KroNI GCCGGC 1 cut(s) 626
Kzo9I GATC 6 cut(s) 485, 658, 775, 853, 918, 1047
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 3 cut(s) 70, 196, 863
Lsp1109I GCAGC 4 cut(s) 506, 805, 827, 944
LweI GCATC 3 cut(s) 434, 540, 587
MaeI CTAG 2 cut(s) 15, 201
MaeII ACGT 1 cut(s) 1056
MaeIII GTNAC 1 cut(s) 290
MalI GATC 6 cut(s) 487, 660, 777, 855, 920, 1049
MboI GATC 6 cut(s) 485, 658, 775, 853, 918, 1047
MboII GAAGA 5 cut(s) 113, 177, 316, 321, 652
MflI RGATCY 2 cut(s) 485, 658
MhlI GDGCHC 1 cut(s) 1112
MluCI AATT 5 cut(s) 370, 564, 753, 804, 884
MlyI GAGTC 1 cut(s) 637
MmeI TCCRAC 1 cut(s) 841
MnlI CCTC 5 cut(s) 47, 61, 612, 967, 1057
Mph1103I ATGCAT 1 cut(s) 427
MroNI GCCGGC 1 cut(s) 624
MroXI GAANNNNTTC 2 cut(s) 756, 1056
MseI TTAA 5 cut(s) 26, 54, 78, 369, 516
MslI CAYNNNNRTG 1 cut(s) 1115
MspA1I CMGCKG 1 cut(s) 818
MspCI CTTAAG 1 cut(s) 25
MspI CCGG 7 cut(s) 167, 194, 295, 309, 547, 625, 993
MspR9I CCNGG 3 cut(s) 547, 597, 993
MvaI CCWGG 1 cut(s) 597
MwoI GCNNNNNNNGC 3 cut(s) 10, 422, 827
NaeI GCCGGC 1 cut(s) 626
NciI CCSGG 2 cut(s) 547, 993
NdeII GATC 6 cut(s) 485, 658, 775, 853, 918, 1047
NgoMIV GCCGGC 1 cut(s) 624
NlaIII CATG 5 cut(s) 322, 354, 640, 1003, 1088
NlaIV GGNNCC 3 cut(s) 192, 530, 660
NmuCI GTSAC 1 cut(s) 290
NsiI ATGCAT 1 cut(s) 427
NspI RCATGY 2 cut(s) 322, 1003
NspV TTCGAA 1 cut(s) 615
PaeI GCATGC 1 cut(s) 322
PagI TCATGA 1 cut(s) 636
PceI AGGCCT 2 cut(s) 31, 1104
PciI ACATGT 1 cut(s) 999
PciSI GCTCTTC 1 cut(s) 329
PdiI GCCGGC 1 cut(s) 626
PdmI GAANNNNTTC 2 cut(s) 756, 1056
PfeI GAWTC 3 cut(s) 535, 617, 741
PflFI GACNNNGTC 1 cut(s) 983
PfoI TCCNGGA 1 cut(s) 595
PinAI ACCGGT 1 cut(s) 166
PkrI GCNGC 4 cut(s) 496, 817, 820, 959
PleI GAGTC 1 cut(s) 637
PpsI GAGTC 1 cut(s) 637
PscI ACATGT 1 cut(s) 999
PshBI ATTAAT 1 cut(s) 369
Psp1406I AACGTT 1 cut(s) 1056
Psp6I CCWGG 1 cut(s) 595
PspEI GGTNACC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 595
PspN4I GGNNCC 3 cut(s) 192, 530, 660
PspPI GGNCC 2 cut(s) 164, 599
PstI CTGCAG 1 cut(s) 823
PsuI RGATCY 2 cut(s) 485, 658
PsyI GACNNNGTC 1 cut(s) 983
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
RseI CAYNNNNRTG 1 cut(s) 1115
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 5 cut(s) 26, 54, 78, 369, 516
SatI GCNGC 4 cut(s) 495, 816, 819, 958
Sau3AI GATC 6 cut(s) 485, 658, 775, 853, 918, 1047
Sau96I GGNCC 2 cut(s) 164, 599
SchI GAGTC 1 cut(s) 637
ScrFI CCNGG 3 cut(s) 547, 597, 993
SduI GDGCHC 1 cut(s) 1112
SfaNI GCATC 3 cut(s) 434, 540, 587
SfcI CTRYAG 2 cut(s) 85, 819
SfuI TTCGAA 1 cut(s) 615
SinI GGWCC 2 cut(s) 164, 599
SmiMI CAYNNNNRTG 1 cut(s) 1115
SmlI CTYRAG 2 cut(s) 25, 771
SmoI CTYRAG 2 cut(s) 25, 771
SpeI ACTAGT 1 cut(s) 200
SphI GCATGC 1 cut(s) 322
Sse9I AATT 5 cut(s) 370, 564, 753, 804, 884
SseBI AGGCCT 2 cut(s) 31, 1104
SsiI CCGC 2 cut(s) 306, 398
SspI AATATT 2 cut(s) 62, 609
SspMI CTAG 2 cut(s) 15, 201
StuI AGGCCT 2 cut(s) 31, 1104
StyD4I CCNGG 3 cut(s) 545, 595, 991
TaaI ACNGT 4 cut(s) 220, 233, 706, 937
TaiI ACGT 1 cut(s) 1059
TaqI TCGA 3 cut(s) 615, 760, 1050
TasI AATT 5 cut(s) 370, 564, 753, 804, 884
TfiI GAWTC 3 cut(s) 535, 617, 741
Tru1I TTAA 5 cut(s) 26, 54, 78, 369, 516
Tru9I TTAA 5 cut(s) 26, 54, 78, 369, 516
TscAI CASTG 2 cut(s) 238, 669
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 4 cut(s) 494, 815, 818, 957
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 5 cut(s) 114, 489, 501, 653, 1073
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 2 cut(s) 238, 669
Tth111I GACNNNGTC 1 cut(s) 983
Vha464I CTTAAG 1 cut(s) 25
VpaK11BI GGWCC 2 cut(s) 164, 599
VspI ATTAAT 1 cut(s) 369
XagI CCTNNNNNAGG 1 cut(s) 18
XceI RCATGY 2 cut(s) 322, 1003
XmnI GAANNNNTTC 2 cut(s) 756, 1056
XspI CTAG 2 cut(s) 15, 201
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.