MD05G1272400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0028794
Physical Location & Seq
Forward (+)
40747320 .. 40747731
412 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1272400.v1.1.491

Sequence Viewer

Length: 375 bp
ATGCTATTCATACTAGGTGGCACAGAGATGGAAATCACAAGACGTGACGAGATAGAAGAACACAGAGAGAGAAAAATATCAAACGAAGAAACAGAGTTAGATACAGATTTTTCCGATCGCGATTCTCGACAGTCTCGACCAATTTCAAGACTCGGAATCGCTGGTCCGGCGAGGGCTGGCTTCGTGGATGCAATCACGGCTCTAATCAAAGCAATCATTAGGATTAAGATTTCGAGAGGCAGCAGAGAATGCATGAGAGCCTTCCCTAATTCGCTGCTTGCATGCCCTTCGTTTCACGGCGGTCGCGGCGCCCTACCCTCGGCCGGTGGGCATCCCTCCGATCTCACCTTCCTTGCCGGCGGTGGTTGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.46

Weight (kDa)

6.83

Isoelectric Point (pI)

61.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028794)

Species Orthologous Gene IDs
malus_domestica MD05G1272400.v1.1
prunus_persica Prupe.4G091000_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 308
AccII CGCG 2 cut(s) 120, 306
AciI CCGC 3 cut(s) 300, 306, 360
AcoI YGGCCR 1 cut(s) 321
AcyI GRCGYC 1 cut(s) 309
AfiI CCNNNNNNNGG 2 cut(s) 319, 323
AgsI TTSAA 1 cut(s) 147
AjiI CACGTC 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 138
AoxI GGCC 1 cut(s) 321
ApeKI GCWGC 3 cut(s) 240, 274, 369
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 337
AvaII GGWCC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 308
BbvI GCAGC 3 cut(s) 252, 261, 356
BccI CCATC 1 cut(s) 22
BceAI ACGGC 2 cut(s) 213, 313
BcgI CGANNNNNNTGC 2 cut(s) 270, 304
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 312
BisI GCNGC 4 cut(s) 241, 275, 307, 370
BlsI GCNGC 4 cut(s) 242, 276, 308, 371
Bme18I GGWCC 1 cut(s) 164
BmgBI CACGTC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 1 cut(s) 310
BmsI GCATC 2 cut(s) 178, 340
BsaBI GATNNNNATC 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 1 cut(s) 318
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 323
Bse118I RCCGGY 2 cut(s) 323, 356
Bse8I GATNNNNATC 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 318
BseGI GGATG 2 cut(s) 193, 331
BseJI GATNNNNATC 1 cut(s) 32
BseLI CCNNNNNNNGG 2 cut(s) 319, 323
BseX3I CGGCCG 1 cut(s) 321
BseXI GCAGC 3 cut(s) 252, 261, 356
Bsh1236I CGCG 2 cut(s) 120, 306
Bsh1285I CGRYCG 3 cut(s) 118, 304, 324
BshFI GGCC 1 cut(s) 323
BshNI GGYRCC 1 cut(s) 308
BsiEI CGRYCG 3 cut(s) 118, 304, 324
BsiSI CCGG 3 cut(s) 167, 324, 357
BslI CCNNNNNNNGG 2 cut(s) 319, 323
BsmAI GTCTC 1 cut(s) 138
BsmI GAATGC 1 cut(s) 254
BsnI GGCC 1 cut(s) 323
Bsp143I GATC 2 cut(s) 115, 340
Bsp68I TCGCGA 1 cut(s) 120
BspACI CCGC 3 cut(s) 300, 306, 360
BspANI GGCC 1 cut(s) 323
BspFNI CGCG 2 cut(s) 120, 306
BspLI GGNNCC 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 308
BsrFI RCCGGY 2 cut(s) 323, 356
BssAI RCCGGY 2 cut(s) 323, 356
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 2 cut(s) 115, 340
BssNI GRCGYC 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 132
BstACI GRCGYC 1 cut(s) 309
BstAPI GCANNNNNTGC 1 cut(s) 249
BstC8I GCNNGC 4 cut(s) 178, 279, 283, 358
BstF5I GGATG 2 cut(s) 193, 331
BstFNI CGCG 2 cut(s) 120, 306
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 2 cut(s) 118, 343
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 2 cut(s) 115, 340
BstMCI CGRYCG 3 cut(s) 118, 304, 324
BstMWI GCNNNNNNNGC 5 cut(s) 167, 197, 249, 306, 366
BstNSI RCATGY 1 cut(s) 285
BstUI CGCG 2 cut(s) 120, 306
BstV1I GCAGC 3 cut(s) 252, 261, 356
BstZI CGGCCG 1 cut(s) 321
BsuRI GGCC 1 cut(s) 323
BtrI CACGTC 1 cut(s) 44
BtsCI GGATG 2 cut(s) 193, 331
BtuMI TCGCGA 1 cut(s) 120
Cac8I GCNNGC 4 cut(s) 178, 279, 283, 358
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 2 cut(s) 323, 356
Cfr13I GGNCC 1 cut(s) 164
CviAII CATG 2 cut(s) 253, 282
CviJI RGCY 5 cut(s) 176, 180, 200, 260, 323
CviKI_1 RGCY 5 cut(s) 176, 180, 200, 260, 323
DinI GGCGCC 1 cut(s) 310
DpnI GATC 2 cut(s) 117, 342
DpnII GATC 2 cut(s) 115, 340
EaeI YGGCCR 1 cut(s) 321
EagI CGGCCG 1 cut(s) 321
EclXI CGGCCG 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 164
Eco52I CGGCCG 1 cut(s) 321
EcoT22I ATGCAT 1 cut(s) 254
EgeI GGCGCC 1 cut(s) 310
EheI GGCGCC 1 cut(s) 310
FaeI CATG 2 cut(s) 256, 285
FaiI YATR 3 cut(s) 11, 254, 283
FatI CATG 2 cut(s) 252, 281
Fnu4HI GCNGC 4 cut(s) 241, 275, 307, 370
FokI GGATG 2 cut(s) 200, 318
Fsp4HI GCNGC 4 cut(s) 241, 275, 307, 370
FspBI CTAG 1 cut(s) 14
GlaI GCGC 1 cut(s) 310
GluI GCNGC 4 cut(s) 241, 275, 307, 370
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 1 cut(s) 323
HapII CCGG 3 cut(s) 167, 324, 357
HhaI GCGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 2 cut(s) 256, 285
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HinfI GANTC 3 cut(s) 122, 150, 156
HpaII CCGG 3 cut(s) 167, 324, 357
HphI GGTGA 1 cut(s) 337
Hpy188I TCNGA 3 cut(s) 115, 155, 340
Hpy188III TCNNGA 5 cut(s) 119, 126, 135, 147, 234
HpyAV CCTTC 3 cut(s) 271, 297, 358
HpyCH4III ACNGT 1 cut(s) 132
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 3 cut(s) 191, 252, 281
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 197, 249, 306, 366
HpySE526I ACGT 1 cut(s) 43
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 2 cut(s) 256, 285
HspAI GCGC 1 cut(s) 309
KasI GGCGCC 1 cut(s) 308
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Kzo9I GATC 2 cut(s) 115, 340
LpnPI CCDG 5 cut(s) 147, 162, 180, 337, 370
Lsp1109I GCAGC 3 cut(s) 252, 261, 356
LweI GCATC 2 cut(s) 178, 340
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 43
MaeIII GTNAC 1 cut(s) 44
MalI GATC 2 cut(s) 117, 342
MboI GATC 2 cut(s) 115, 340
MboII GAAGA 2 cut(s) 68, 98
MluCI AATT 2 cut(s) 141, 268
Mly113I GGCGCC 1 cut(s) 309
MlyI GAGTC 1 cut(s) 144
MnlI CCTC 4 cut(s) 165, 230, 328, 346
Mph1103I ATGCAT 1 cut(s) 254
MroNI GCCGGC 1 cut(s) 356
MseI TTAA 1 cut(s) 225
MslI CAYNNNNRTG 1 cut(s) 26
MspI CCGG 3 cut(s) 167, 324, 357
Mva1269I GAATGC 1 cut(s) 254
MvnI CGCG 2 cut(s) 120, 306
MwoI GCNNNNNNNGC 5 cut(s) 167, 197, 249, 306, 366
NaeI GCCGGC 1 cut(s) 358
NarI GGCGCC 1 cut(s) 309
NdeII GATC 2 cut(s) 115, 340
NgoMIV GCCGGC 1 cut(s) 356
NlaIII CATG 2 cut(s) 256, 285
NlaIV GGNNCC 1 cut(s) 310
NmeAIII GCCGAG 1 cut(s) 299
NmuCI GTSAC 1 cut(s) 44
NruI TCGCGA 1 cut(s) 120
NsiI ATGCAT 1 cut(s) 254
NspI RCATGY 1 cut(s) 285
PaeI GCATGC 1 cut(s) 285
PcsI WCGNNNNNNNCGW 2 cut(s) 124, 133
PctI GAATGC 1 cut(s) 254
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 2 cut(s) 122, 156
PkrI GCNGC 4 cut(s) 242, 276, 308, 371
Ple19I CGATCG 1 cut(s) 118
PleI GAGTC 1 cut(s) 144
PluTI GGCGCC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 310
PspPI GGNCC 1 cut(s) 164
PvuI CGATCG 1 cut(s) 118
RruI TCGCGA 1 cut(s) 120
RseI CAYNNNNRTG 1 cut(s) 26
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 4 cut(s) 241, 275, 307, 370
Sau3AI GATC 2 cut(s) 115, 340
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 1 cut(s) 144
SetI ASST 3 cut(s) 19, 46, 350
SfaNI GCATC 2 cut(s) 178, 340
SfoI GGCGCC 1 cut(s) 310
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 26
SphI GCATGC 1 cut(s) 285
Sse9I AATT 2 cut(s) 141, 268
SsiI CCGC 3 cut(s) 300, 306, 360
SspDI GGCGCC 1 cut(s) 308
SspMI CTAG 1 cut(s) 14
TaaI ACNGT 1 cut(s) 132
TaiI ACGT 1 cut(s) 46
TaqI TCGA 3 cut(s) 127, 136, 233
TasI AATT 2 cut(s) 141, 268
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 2 cut(s) 122, 156
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 3 cut(s) 240, 274, 369
Tsp45I GTSAC 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 164
XceI RCATGY 1 cut(s) 285
XspI CTAG 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.