MD05G1293600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024431
Physical Location & Seq
Forward (+)
42624407 .. 42626971
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1293600.v1.1.491

Sequence Viewer

Length: 993 bp
ATGATGATCGGTTTTCTGGTGGTTTCAATATCACGATCTTGTGCTTATGGAAAGCCGGAAGTACCTTGTTTTTTCATTTTTGGTGATTCATTGCTCGATAATGGCAACAATAACTTCCTCCCAACGTTGAGAGTGAACTACCTCCCCTACGGCATTGATTATCCCACCGGACCAACCGGAAGATTTTGCAATGGTCGAACTCCAGTTGACGTACTTGCTGAACTTTTGGGCTTTGAGAATCACATCCCACCATTTGCAACTACTTTCGGCCTGGCCACACTTAGAGGCTTGAATTACGCATCAGGCACTTCAGGAATTCGAGCGGAAACAGGCTCTCAGTTGGGTGTTAATGTCAACTTTGATCGGCAGATACAGAACCACAAATCTGCAGTCTTGCGCATTGCTTCCATACTGAGAAGTAAAGAATCAGCTATGAAGTATTTAAACGAGTGCTTATATTATGTGGGAATGGGTAACAATGATTACCTTAACAATTACTTACAGCCGCGGTTTTTTAACACCAGCCGCATATATACCCTTGAACAATATGCTGCAGTTCTTGTTAGACAATATTCCAGCCAAATCATGACTTTGTACAAGTATGGAGCGAGGAAGTTTGCCTTGGTTGGAGTGCAAGATATAGCAAATACGACCTATGCTTTTAACCAAAATCTTGTATCCCTGGTAGAAAAGCTCAACGCCGATCTTGGAGATGCAAGGTTCATCTATGTCAACTCTTCCTCAGTGACACGTTCAACTTCTGCTGGATTCAAGGATTATGACACTAGCTGCTGTCCGGTAAATCGACTAGGTCTATGTGCTATTTTCCAAATTCCATGCCAGAATAGGAGTGAGCATGCATTATGGGACTCATTCCATCCCACTGAGGCCGCCAACATAGTCTCTGCAAGAAGAACATACAATTCCTCAGACCCGTCGGACACTTATCCGATGGATATCAGTCACCTGGTTAACCTCAGCCTAGAATCCTGA

Protein Analysis

331

Amino Acids

36.77

Weight (kDa)

7.54

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 25 - 303 5.1e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024431)

Species Orthologous Gene IDs
malus_domestica MD00G1094000.v1.1 MD05G1293600.v1.1
prunus_persica Prupe.4G070600_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 398
AccBSI CCGCTC 1 cut(s) 323
AccII CGCG 1 cut(s) 508
AciI CCGC 5 cut(s) 323, 506, 508, 526, 891
AclI AACGTT 1 cut(s) 125
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 2 cut(s) 315, 831
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 3 cut(s) 63, 213, 596
AflIII ACRYGT 1 cut(s) 749
AgsI TTSAA 5 cut(s) 27, 292, 542, 756, 772
AjnI CCWGG 3 cut(s) 270, 681, 966
AjuI GAANNNNNNNTTGG 2 cut(s) 605, 637
AluBI AGCT 3 cut(s) 431, 694, 789
AluI AGCT 3 cut(s) 431, 694, 789
Alw26I GTCTC 1 cut(s) 907
AoxI GGCC 3 cut(s) 268, 273, 888
ApeKI GCWGC 2 cut(s) 551, 789
ApoI RAATTY 2 cut(s) 315, 831
ArsI GACNNNNNNTTYG 2 cut(s) 375, 407
AspLEI GCGC 1 cut(s) 399
AspS9I GGNCC 1 cut(s) 170
AsuHPI GGTGA 2 cut(s) 95, 956
AvaII GGWCC 1 cut(s) 170
BalI TGGCCA 1 cut(s) 275
BbvCI CCTCAGC 1 cut(s) 977
BbvI GCAGC 2 cut(s) 538, 776
BccI CCATC 2 cut(s) 885, 946
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 3 cut(s) 272, 683, 968
BciVI GTATCC 1 cut(s) 688
BcoDI GTCTC 1 cut(s) 907
BfaI CTAG 3 cut(s) 786, 809, 983
BfmI CTRYAG 2 cut(s) 387, 552
BfuI GTATCC 1 cut(s) 688
BisI GCNGC 5 cut(s) 506, 526, 552, 790, 891
BlsI GCNGC 5 cut(s) 507, 527, 553, 791, 892
Bme1390I CCNGG 3 cut(s) 272, 683, 968
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 170
BmrFI CCNGG 3 cut(s) 272, 683, 968
BmsI GCATC 2 cut(s) 308, 703
BpmI CTGGAG 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 977
BsaBI GATNNNNATC 1 cut(s) 956
BsaJI CCNNGG 3 cut(s) 506, 621, 681
BsaWI WCCGGW 3 cut(s) 167, 176, 796
Bse1I ACTGG 1 cut(s) 203
Bse3DI GCAATG 3 cut(s) 89, 196, 399
Bse8I GATNNNNATC 1 cut(s) 956
BseBI CCWGG 3 cut(s) 272, 683, 968
BseDI CCNNGG 3 cut(s) 506, 621, 681
BseGI GGATG 2 cut(s) 243, 877
BseJI GATNNNNATC 1 cut(s) 956
BseMI GCAATG 3 cut(s) 89, 196, 399
BseMII CTCAG 6 cut(s) 350, 404, 756, 876, 942, 991
BseNI ACTGG 1 cut(s) 203
BseXI GCAGC 2 cut(s) 538, 776
Bsh1236I CGCG 1 cut(s) 508
BshFI GGCC 3 cut(s) 270, 275, 890
BsiSI CCGG 4 cut(s) 56, 168, 177, 797
BslFI GGGAC 1 cut(s) 881
BsmAI GTCTC 1 cut(s) 907
BsmFI GGGAC 1 cut(s) 881
BsnI GGCC 3 cut(s) 270, 275, 890
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 4 cut(s) 6, 35, 361, 703
BspACI CCGC 5 cut(s) 323, 506, 508, 526, 891
BspANI GGCC 3 cut(s) 270, 275, 890
BspCNI CTCAG 6 cut(s) 349, 405, 755, 877, 941, 990
BspFNI CGCG 1 cut(s) 508
BspHI TCATGA 1 cut(s) 585
BspMAI CTGCAG 2 cut(s) 391, 556
BsrBI CCGCTC 1 cut(s) 323
BsrDI GCAATG 3 cut(s) 89, 196, 399
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 1 cut(s) 203
BssECI CCNNGG 3 cut(s) 506, 621, 681
BssMI GATC 4 cut(s) 6, 35, 361, 703
BssT1I CCWWGG 1 cut(s) 621
Bst2UI CCWGG 3 cut(s) 272, 683, 968
Bst6I CTCTTC 1 cut(s) 742
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 1 cut(s) 858
BstDEI CTNAG 7 cut(s) 281, 336, 413, 742, 885, 928, 977
BstDSI CCRYGG 1 cut(s) 506
BstF5I GGATG 2 cut(s) 243, 877
BstFNI CGCG 1 cut(s) 508
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 4 cut(s) 9, 38, 364, 706
BstMAI GTCTC 1 cut(s) 907
BstMBI GATC 4 cut(s) 6, 35, 361, 703
BstNI CCWGG 3 cut(s) 272, 683, 968
BstNSI RCATGY 1 cut(s) 860
BstSCI CCNGG 3 cut(s) 270, 681, 966
BstSFI CTRYAG 2 cut(s) 387, 552
BstUI CGCG 1 cut(s) 508
BstV1I GCAGC 2 cut(s) 538, 776
BsuI GTATCC 1 cut(s) 688
BsuRI GGCC 3 cut(s) 270, 275, 890
BtgI CCRYGG 1 cut(s) 506
BtsCI GGATG 2 cut(s) 243, 877
BtsIMutI CAGTG 2 cut(s) 750, 882
Cac8I GCNNGC 1 cut(s) 858
CciI TCATGA 1 cut(s) 585
CfoI GCGC 1 cut(s) 399
Cfr13I GGNCC 1 cut(s) 170
Cfr42I CCGCGG 1 cut(s) 509
CsiI ACCWGGT 1 cut(s) 966
Csp6I GTAC 3 cut(s) 62, 212, 595
CviAII CATG 3 cut(s) 586, 837, 857
CviQI GTAC 3 cut(s) 62, 212, 595
DdeI CTNAG 7 cut(s) 281, 336, 413, 742, 885, 928, 977
DpnI GATC 4 cut(s) 8, 37, 363, 705
DpnII GATC 4 cut(s) 6, 35, 361, 703
DraI TTTAAA 1 cut(s) 444
EaeI YGGCCR 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 742
EarI CTCTTC 1 cut(s) 742
Eco130I CCWWGG 1 cut(s) 621
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 294
EcoRI GAATTC 1 cut(s) 315
EcoRII CCWGG 3 cut(s) 270, 681, 966
EcoRV GATATC 1 cut(s) 958
EcoT14I CCWWGG 1 cut(s) 621
EcoT22I ATGCAT 1 cut(s) 862
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 3 cut(s) 589, 840, 860
FalI AAGNNNNNCTT 2 cut(s) 605, 637
FaqI GGGAC 1 cut(s) 881
FatI CATG 3 cut(s) 585, 836, 856
Fnu4HI GCNGC 5 cut(s) 506, 526, 552, 790, 891
FokI GGATG 2 cut(s) 230, 864
Fsp4HI GCNGC 5 cut(s) 506, 526, 552, 790, 891
FspBI CTAG 3 cut(s) 786, 809, 983
FspI TGCGCA 1 cut(s) 398
GlaI GCGC 1 cut(s) 398
GluI GCNGC 5 cut(s) 506, 526, 552, 790, 891
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 3 cut(s) 270, 275, 890
HapII CCGG 4 cut(s) 56, 168, 177, 797
HhaI GCGC 1 cut(s) 399
Hin1II CATG 3 cut(s) 589, 840, 860
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HincII GTYRAC 4 cut(s) 208, 355, 733, 973
HindII GTYRAC 4 cut(s) 208, 355, 733, 973
HinfI GANTC 6 cut(s) 86, 238, 425, 768, 869, 986
HpaI GTTAAC 1 cut(s) 973
HpaII CCGG 4 cut(s) 56, 168, 177, 797
HphI GGTGA 2 cut(s) 95, 956
Hpy166II GTNNAC 5 cut(s) 136, 208, 355, 733, 973
Hpy188I TCNGA 3 cut(s) 931, 940, 951
Hpy188III TCNNGA 4 cut(s) 33, 312, 586, 990
Hpy8I GTNNAC 5 cut(s) 136, 208, 355, 733, 973
Hpy99I CGWCG 1 cut(s) 940
HpyCH4IV ACGT 3 cut(s) 125, 210, 751
HpyCH4V TGCA 8 cut(s) 189, 257, 389, 554, 634, 716, 860, 908
HpyF3I CTNAG 7 cut(s) 281, 336, 413, 742, 885, 928, 977
HpySE526I ACGT 3 cut(s) 125, 210, 751
Hsp92II CATG 3 cut(s) 589, 840, 860
HspAI GCGC 1 cut(s) 397
KspAI GTTAAC 1 cut(s) 973
KspI CCGCGG 1 cut(s) 509
Kzo9I GATC 4 cut(s) 6, 35, 361, 703
LmnI GCTCC 1 cut(s) 605
Lsp1109I GCAGC 2 cut(s) 538, 776
LweI GCATC 2 cut(s) 308, 703
MabI ACCWGGT 1 cut(s) 966
MaeI CTAG 3 cut(s) 786, 809, 983
MaeII ACGT 3 cut(s) 125, 210, 751
MaeIII GTNAC 3 cut(s) 473, 745, 962
MalI GATC 4 cut(s) 8, 37, 363, 705
MbiI CCGCTC 1 cut(s) 323
MboI GATC 4 cut(s) 6, 35, 361, 703
MboII GAAGA 3 cut(s) 192, 729, 924
MlsI TGGCCA 1 cut(s) 275
MluCI AATT 5 cut(s) 292, 315, 493, 831, 922
MluNI TGGCCA 1 cut(s) 275
MlyI GAGTC 1 cut(s) 863
MmeI TCCRAC 2 cut(s) 607, 918
MnlI CCTC 8 cut(s) 128, 152, 278, 603, 751, 880, 937, 986
Mox20I TGGCCA 1 cut(s) 275
Mph1103I ATGCAT 1 cut(s) 862
MscI TGGCCA 1 cut(s) 275
MseI TTAA 6 cut(s) 348, 443, 489, 516, 663, 972
Msp20I TGGCCA 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 4 cut(s) 56, 168, 177, 797
MspR9I CCNGG 3 cut(s) 272, 683, 968
MvaI CCWGG 3 cut(s) 272, 683, 968
MvnI CGCG 1 cut(s) 508
NdeII GATC 4 cut(s) 6, 35, 361, 703
NlaIII CATG 3 cut(s) 589, 840, 860
NmuCI GTSAC 2 cut(s) 745, 962
NsbI TGCGCA 1 cut(s) 398
NsiI ATGCAT 1 cut(s) 862
NspI RCATGY 1 cut(s) 860
PaeI GCATGC 1 cut(s) 860
PagI TCATGA 1 cut(s) 585
PfeI GAWTC 5 cut(s) 86, 238, 425, 768, 986
PflFI GACNNNGTC 1 cut(s) 810
PkrI GCNGC 5 cut(s) 507, 527, 553, 791, 892
PleI GAGTC 1 cut(s) 863
PpsI GAGTC 1 cut(s) 863
Psp1406I AACGTT 1 cut(s) 125
Psp6I CCWGG 3 cut(s) 270, 681, 966
PspGI CCWGG 3 cut(s) 270, 681, 966
PspPI GGNCC 1 cut(s) 170
PstI CTGCAG 2 cut(s) 391, 556
PsyI GACNNNGTC 1 cut(s) 810
RsaI GTAC 3 cut(s) 63, 213, 596
RsaNI GTAC 3 cut(s) 62, 212, 595
SacII CCGCGG 1 cut(s) 509
SaqAI TTAA 6 cut(s) 348, 443, 489, 516, 663, 972
SatI GCNGC 5 cut(s) 506, 526, 552, 790, 891
Sau3AI GATC 4 cut(s) 6, 35, 361, 703
Sau96I GGNCC 1 cut(s) 170
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 3 cut(s) 272, 683, 968
SexAI ACCWGGT 1 cut(s) 966
SfaNI GCATC 2 cut(s) 308, 703
SfcI CTRYAG 2 cut(s) 387, 552
Sfr303I CCGCGG 1 cut(s) 509
SgrBI CCGCGG 1 cut(s) 509
SinI GGWCC 1 cut(s) 170
SphI GCATGC 1 cut(s) 860
Sse9I AATT 5 cut(s) 292, 315, 493, 831, 922
SsiI CCGC 5 cut(s) 323, 506, 508, 526, 891
SspI AATATT 1 cut(s) 572
SspMI CTAG 3 cut(s) 786, 809, 983
StyD4I CCNGG 3 cut(s) 270, 681, 966
StyI CCWWGG 1 cut(s) 621
TaiI ACGT 3 cut(s) 128, 213, 754
TaqI TCGA 4 cut(s) 96, 196, 319, 805
TasI AATT 5 cut(s) 292, 315, 493, 831, 922
TatI WGTACW 1 cut(s) 594
TauI GCSGC 3 cut(s) 508, 528, 893
TfiI GAWTC 5 cut(s) 86, 238, 425, 768, 986
Tru1I TTAA 6 cut(s) 348, 443, 489, 516, 663, 972
Tru9I TTAA 6 cut(s) 348, 443, 489, 516, 663, 972
TscAI CASTG 2 cut(s) 750, 889
TseFI GTSAC 2 cut(s) 745, 962
TseI GCWGC 2 cut(s) 551, 789
Tsp45I GTSAC 2 cut(s) 745, 962
TspDTI ATGAA 4 cut(s) 64, 78, 449, 712
TspRI CASTG 2 cut(s) 750, 889
Tth111I GACNNNGTC 1 cut(s) 810
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 2 cut(s) 315, 831
XceI RCATGY 1 cut(s) 860
XspI CTAG 3 cut(s) 786, 809, 983
Zsp2I ATGCAT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.