MD05G1305300.v1.1

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0028809
Physical Location & Seq
Reverse (-)
43758839 .. 43759324
486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1305300.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGGGGGAAGGCTTAACATTCCGCCGGAGGTTTTCATGGTTCGACCCGACGGAACAGGGAGAACTATGATTCACTCAGGCGCGCCGTTCACGATCCTTATAAGGCCTGCTTACGATGTATTAGTAGCAGGTTTAGAAGCAGCGTTCTCGGTGATGGGTGAAGCGACCGTGAGGAGGATCCCTAGCGATTTTAAGCCTTTGGAGCCGTGCCACGAGGTGGCGAACCGGCAAGCTTTTACGGGGTTTCCATGGATCACCTTCCATTTCGACAACAATGCCGATCTGAATTTGGATGCGTTGAATGCTTTCTATATGGTGAAGAATGAAGCTGGAAACATCGAGTTTTGCTTTGCAATGATTCCTGACGATAATCCTCATGGTATGACCTCAATCGGAGCGTTCCAACAGACTAATCACCGCTTCATATATGACACAAAAGGATTCAAGCTGTTGTTTGGTCATGAGAATTGTTTTGAGACTTCTTAA

Protein Analysis

162

Amino Acids

18.01

Weight (kDa)

5.41

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_C PF14541 3 - 152 1.4e-23 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028809)

Species Orthologous Gene IDs
malus_domestica MD05G1305300.v1.1
prunus_persica Prupe.4G056900_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 119
AccB7I CCANNNNNTGG 1 cut(s) 217
AccII CGCG 1 cut(s) 83
AciI CCGC 2 cut(s) 23, 418
AclWI GGATC 4 cut(s) 88, 172, 185, 260
AcsI RAATTY 1 cut(s) 286
AdeI CACNNNGTG 1 cut(s) 217
AfiI CCNNNNNNNGG 2 cut(s) 174, 217
AgsI TTSAA 2 cut(s) 301, 445
AluBI AGCT 3 cut(s) 233, 329, 448
AluI AGCT 3 cut(s) 233, 329, 448
Alw26I GTCTC 1 cut(s) 470
AlwI GGATC 4 cut(s) 88, 172, 185, 260
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 1 cut(s) 286
AscI GGCGCGCC 1 cut(s) 81
Asp700I GAANNNNTTC 1 cut(s) 305
AspLEI GCGC 2 cut(s) 83, 85
AsuHPI GGTGA 5 cut(s) 163, 170, 247, 328, 407
BamHI GGATCC 1 cut(s) 177
BauI CACGAG 1 cut(s) 212
BbvI GCAGC 1 cut(s) 152
BccI CCATC 1 cut(s) 148
BceAI ACGGC 2 cut(s) 70, 190
BcgI CGANNNNNNTGC 4 cut(s) 129, 163, 257, 291
BcoDI GTCTC 1 cut(s) 470
BfaI CTAG 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 119
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmiI GGNNCC 2 cut(s) 179, 204
BmsI GCATC 1 cut(s) 283
BsaJI CCNNGG 1 cut(s) 248
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 217
Bse118I RCCGGY 1 cut(s) 225
Bse3DI GCAATG 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 248
BseGI GGATG 1 cut(s) 298
BseLI CCNNNNNNNGG 2 cut(s) 174, 217
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 1 cut(s) 90
BsePI GCGCGC 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 187
BseXI GCAGC 1 cut(s) 152
Bsh1236I CGCG 1 cut(s) 83
Bsh1285I CGRYCG 1 cut(s) 168
BshFI GGCC 1 cut(s) 106
BsiEI CGRYCG 1 cut(s) 168
BsiSI CCGG 2 cut(s) 26, 226
BslI CCNNNNNNNGG 2 cut(s) 174, 217
BsmAI GTCTC 1 cut(s) 470
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 1 cut(s) 106
Bsp143I GATC 4 cut(s) 93, 177, 252, 280
Bsp19I CCATGG 1 cut(s) 248
BspACI CCGC 2 cut(s) 23, 418
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 1 cut(s) 89
BspFNI CGCG 1 cut(s) 83
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 2 cut(s) 179, 204
BspMI ACCTGC 1 cut(s) 119
BspPI GGATC 4 cut(s) 88, 172, 185, 260
BsrDI GCAATG 1 cut(s) 360
BsrFI RCCGGY 1 cut(s) 225
BssAI RCCGGY 1 cut(s) 225
BssECI CCNNGG 1 cut(s) 248
BssHII GCGCGC 1 cut(s) 81
BssMI GATC 4 cut(s) 93, 177, 252, 280
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 248
Bst2BI CACGAG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 169
BstC8I GCNNGC 3 cut(s) 83, 108, 231
BstDEI CTNAG 1 cut(s) 76
BstDSI CCRYGG 1 cut(s) 248
BstF5I GGATG 1 cut(s) 298
BstFNI CGCG 1 cut(s) 83
BstHHI GCGC 2 cut(s) 83, 85
BstKTI GATC 4 cut(s) 96, 180, 255, 283
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 4 cut(s) 93, 177, 252, 280
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 2 cut(s) 202, 302
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BsuRI GGCC 1 cut(s) 106
BtgI CCRYGG 1 cut(s) 248
BtsCI GGATG 1 cut(s) 298
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 3 cut(s) 83, 108, 231
CciI TCATGA 1 cut(s) 460
CfoI GCGC 2 cut(s) 83, 85
Cfr10I RCCGGY 1 cut(s) 225
CviAII CATG 4 cut(s) 37, 249, 377, 461
CviJI RGCY 7 cut(s) 13, 106, 196, 205, 233, 329, 448
CviKI_1 RGCY 7 cut(s) 13, 106, 196, 205, 233, 329, 448
DdeI CTNAG 1 cut(s) 76
DpnI GATC 4 cut(s) 95, 179, 254, 282
DpnII GATC 4 cut(s) 93, 177, 252, 280
DraIII CACNNNGTG 1 cut(s) 217
EciI GGCGGA 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 248
Eco147I AGGCCT 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 248
ErhI CCWWGG 1 cut(s) 248
FaeI CATG 4 cut(s) 40, 252, 380, 464
FalI AAGNNNNNCTT 2 cut(s) 94, 126
FatI CATG 4 cut(s) 36, 248, 376, 460
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 305
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 183
GlaI GCGC 2 cut(s) 82, 84
GluI GCNGC 1 cut(s) 141
HaeIII GGCC 1 cut(s) 106
HapII CCGG 2 cut(s) 26, 226
HhaI GCGC 2 cut(s) 83, 85
Hin1II CATG 4 cut(s) 40, 252, 380, 464
Hin6I GCGC 2 cut(s) 81, 83
HinP1I GCGC 2 cut(s) 81, 83
HindIII AAGCTT 1 cut(s) 231
HinfI GANTC 3 cut(s) 70, 358, 441
HpaII CCGG 2 cut(s) 26, 226
HphI GGTGA 5 cut(s) 163, 170, 247, 328, 407
Hpy166II GTNNAC 1 cut(s) 90
Hpy188I TCNGA 2 cut(s) 285, 395
Hpy188III TCNNGA 3 cut(s) 91, 362, 461
Hpy8I GTNNAC 1 cut(s) 90
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 3, 268
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4V TGCA 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 2 cut(s) 202, 302
HpyF3I CTNAG 1 cut(s) 76
Hsp92II CATG 4 cut(s) 40, 252, 380, 464
HspAI GCGC 2 cut(s) 81, 83
Kzo9I GATC 4 cut(s) 93, 177, 252, 280
LmnI GCTCC 2 cut(s) 202, 395
LpnPI CCDG 8 cut(s) 39, 42, 63, 114, 120, 239, 315, 375
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 1 cut(s) 283
MaeI CTAG 1 cut(s) 183
MalI GATC 4 cut(s) 95, 179, 254, 282
MboI GATC 4 cut(s) 93, 177, 252, 280
MboII GAAGA 1 cut(s) 331
MflI RGATCY 1 cut(s) 177
MluCI AATT 2 cut(s) 286, 466
MmeI TCCRAC 1 cut(s) 427
MnlI CCTC 6 cut(s) 22, 165, 168, 208, 384, 397
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 3 cut(s) 15, 192, 484
MspI CCGG 2 cut(s) 26, 226
Mva1269I GAATGC 1 cut(s) 307
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 2 cut(s) 202, 302
NcoI CCATGG 1 cut(s) 248
NdeII GATC 4 cut(s) 93, 177, 252, 280
NlaIII CATG 4 cut(s) 40, 252, 380, 464
NlaIV GGNNCC 2 cut(s) 179, 204
PagI TCATGA 1 cut(s) 460
PalAI GGCGCGCC 1 cut(s) 81
PauI GCGCGC 1 cut(s) 81
PceI AGGCCT 1 cut(s) 106
PctI GAATGC 1 cut(s) 307
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 3 cut(s) 70, 358, 441
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 1 cut(s) 142
PsiI TTATAA 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 179, 204
PsuI RGATCY 1 cut(s) 177
PteI GCGCGC 1 cut(s) 81
SaqAI TTAA 3 cut(s) 15, 192, 484
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 4 cut(s) 93, 177, 252, 280
SetI ASST 8 cut(s) 33, 133, 219, 235, 260, 331, 389, 450
SfaNI GCATC 1 cut(s) 283
SgsI GGCGCGCC 1 cut(s) 81
Sse9I AATT 2 cut(s) 286, 466
SseBI AGGCCT 1 cut(s) 106
SsiI CCGC 2 cut(s) 23, 418
SspMI CTAG 1 cut(s) 183
StuI AGGCCT 1 cut(s) 106
StyI CCWWGG 1 cut(s) 248
TaaI ACNGT 1 cut(s) 169
TaqI TCGA 3 cut(s) 43, 267, 339
TasI AATT 2 cut(s) 286, 466
TfiI GAWTC 3 cut(s) 70, 358, 441
Tru1I TTAA 3 cut(s) 15, 192, 484
Tru9I TTAA 3 cut(s) 15, 192, 484
TseI GCWGC 1 cut(s) 140
TspDTI ATGAA 3 cut(s) 25, 339, 412
TspGWI ACGGA 1 cut(s) 66
Van91I CCANNNNNTGG 1 cut(s) 217
XapI RAATTY 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.