MD05G1338900.v1.1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024455
Physical Location & Seq
Forward (+)
46017088 .. 46018919
1832 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1338900.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGATGAAATCTGTAGAAACACGACAAGATATTTGAGTCTTCTGACACTTATATTAGGAATACGATCCCGTCCATTTATGTACTCCTTTCGTTTGGATATGGAACATCTGATTGAAAAGCAAATTGTAACCAGTCCACACATCAATGCTTACGCTTGCATGAAAACAAACATTTATTTCAGGTTCTTGTGGAGAAAGAATGCAACTTTTGCAATTTCTAGACATTATAAACGCTTTGATGTGTTTACGGAAGCTGAGGCAAATAAAGCTGCTGGAAAATACGACAATGCCTCTATTGACTTCCAGGTTGAATTTTACAAAAAGGAAGGTTTGACCCCATATTCTGAGGAAAAGCTTCCCATTACTAGTGACGTTCCTGAAGGATGTGTTATACTAAGGGAGCATGTCCCCATAAGCAACCTCGCCGCTTGTCTTTGGTTCCATGAAGTTGATCGTTTTACTTCCAGGGATCAAATTAGTTTTTCCATAGTGAGAGATCACATTCGGGCAAAGACAAACTGGACTATCAGTATGTTCTACGACTGTGAAAGGCGCAATTTTGTCGTTCAGAAATATCATAGAGATGTATGGGAGCACATGGCTCCTCGTCTTCCTAAGGCTGTTCCTCCACCACCGCTATTACCACCACCACCTCCCTCACCCTCACTCCCAGCTTTAAGTAACGAACCGCCAGTTAAACCATCAACCGAAACTTCACCCGAAAGGGTTGTAAACGCCCCAGCTAAGAGGCCTCCAAGGCGCGGGAGAGATAAGAGGCCGGGCTCTAGACGTCACCGGAAAGTTGTTGCCGGTGGTAGAGACATTGATTCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

279

Amino Acids

32.21

Weight (kDa)

9.63

Isoelectric Point (pI)

74.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 58 - 193 5.4e-62 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024455)

Species Orthologous Gene IDs
malus_domestica MD05G1338900.v1.1
pyrus_communis pycom10g24580
rosa_multiflora Rmu_co8449883.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
AatII GACGTC 1 cut(s) 793
AccII CGCG 1 cut(s) 762
AciI CCGC 4 cut(s) 426, 635, 689, 762
AclWI GGATC 2 cut(s) 60, 477
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 1 cut(s) 399
AcyI GRCGYC 1 cut(s) 790
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 761
AgsI TTSAA 2 cut(s) 116, 311
AhlI ACTAGT 1 cut(s) 365
AjnI CCWGG 2 cut(s) 303, 464
AluBI AGCT 5 cut(s) 254, 269, 355, 674, 743
AluI AGCT 5 cut(s) 254, 269, 355, 674, 743
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 1 cut(s) 813
AlwI GGATC 2 cut(s) 60, 477
AoxI GGCC 2 cut(s) 749, 776
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 1 cut(s) 311
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
Asp700I GAANNNNTTC 1 cut(s) 354
AspLEI GCGC 2 cut(s) 555, 762
AsuC2I CCSGG 1 cut(s) 780
AsuHPI GGTGA 3 cut(s) 651, 708, 785
AxyI CCTNAGG 1 cut(s) 615
BanII GRGCYC 1 cut(s) 785
BbsI GAAGAC 2 cut(s) 32, 602
Bbv12I GWGCWC 1 cut(s) 597
BbvCI CCTCAGC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 256
BccI CCATC 1 cut(s) 709
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 2 cut(s) 305, 466
BcnI CCSGG 1 cut(s) 780
BcoDI GTCTC 1 cut(s) 813
BcuI ACTAGT 1 cut(s) 365
BfaI CTAG 3 cut(s) 219, 366, 786
BfmI CTRYAG 1 cut(s) 13
BglI GCCNNNNNGGC 1 cut(s) 757
BisI GCNGC 2 cut(s) 270, 426
BlsI GCNGC 2 cut(s) 271, 427
Bme1390I CCNGG 3 cut(s) 305, 466, 780
BmiI GGNNCC 2 cut(s) 440, 603
BmrFI CCNGG 3 cut(s) 305, 466, 780
BpiI GAAGAC 2 cut(s) 32, 602
Bpu10I CCTNAGC 1 cut(s) 255
BpuMI CCSGG 1 cut(s) 780
BsaHI GRCGYC 1 cut(s) 790
BsaJI CCNNGG 2 cut(s) 465, 755
BsaWI WCCGGW 1 cut(s) 795
BsaXI ACNNNNNCTCC 2 cut(s) 651, 681
Bsc4I CCNNNNNNNGG 1 cut(s) 761
Bse118I RCCGGY 1 cut(s) 809
Bse1I ACTGG 3 cut(s) 132, 524, 692
Bse21I CCTNAGG 1 cut(s) 615
BseBI CCWGG 2 cut(s) 305, 466
BseDI CCNNGG 2 cut(s) 465, 755
BseGI GGATG 2 cut(s) 10, 389
BseLI CCNNNNNNNGG 1 cut(s) 761
BseMII CTCAG 2 cut(s) 246, 336
BseNI ACTGG 3 cut(s) 132, 524, 692
BseRI GAGGAG 1 cut(s) 594
BseXI GCAGC 1 cut(s) 256
BseYI CCCAGC 2 cut(s) 670, 739
Bsh1236I CGCG 1 cut(s) 762
BshFI GGCC 2 cut(s) 751, 778
BsiHKAI GWGCWC 1 cut(s) 597
BsiSI CCGG 3 cut(s) 779, 796, 810
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 1 cut(s) 761
BsmAI GTCTC 1 cut(s) 813
BsmFI GGGAC 1 cut(s) 392
BsmI GAATGC 1 cut(s) 205
BsnI GGCC 2 cut(s) 751, 778
Bsp1286I GDGCHC 2 cut(s) 597, 785
Bsp143I GATC 4 cut(s) 65, 451, 469, 496
BspACI CCGC 4 cut(s) 426, 635, 689, 762
BspANI GGCC 2 cut(s) 751, 778
BspCNI CTCAG 2 cut(s) 247, 337
BspFNI CGCG 1 cut(s) 762
BspLI GGNNCC 2 cut(s) 440, 603
BspPI GGATC 2 cut(s) 60, 477
BsrFI RCCGGY 1 cut(s) 809
BsrI ACTGG 3 cut(s) 132, 524, 692
BssAI RCCGGY 1 cut(s) 809
BssECI CCNNGG 2 cut(s) 465, 755
BssMI GATC 4 cut(s) 65, 451, 469, 496
BssNI GRCGYC 1 cut(s) 790
BssT1I CCWWGG 1 cut(s) 755
Bst2UI CCWGG 2 cut(s) 305, 466
Bst4CI ACNGT 1 cut(s) 545
BstACI GRCGYC 1 cut(s) 790
BstAPI GCANNNNNTGC 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 5 cut(s) 255, 345, 395, 615, 744
BstF5I GGATG 2 cut(s) 10, 389
BstFNI CGCG 1 cut(s) 762
BstHHI GCGC 2 cut(s) 555, 762
BstKTI GATC 4 cut(s) 68, 454, 472, 499
BstMAI GTCTC 1 cut(s) 813
BstMBI GATC 4 cut(s) 65, 451, 469, 496
BstMWI GCNNNNNNNGC 3 cut(s) 209, 266, 757
BstNI CCWGG 2 cut(s) 305, 466
BstNSI RCATGY 1 cut(s) 407
BstSCI CCNGG 3 cut(s) 303, 464, 778
BstSFI CTRYAG 1 cut(s) 13
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 1 cut(s) 256
BstV2I GAAGAC 2 cut(s) 32, 602
Bsu36I CCTNAGG 1 cut(s) 615
BsuRI GGCC 2 cut(s) 751, 778
BtsCI GGATG 2 cut(s) 10, 389
Cac8I GCNNGC 1 cut(s) 157
CfoI GCGC 2 cut(s) 555, 762
Cfr10I RCCGGY 1 cut(s) 809
Csp6I GTAC 1 cut(s) 82
CviAII CATG 4 cut(s) 160, 404, 443, 598
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 5 cut(s) 255, 345, 395, 615, 744
DpnI GATC 4 cut(s) 67, 453, 471, 498
DpnII GATC 4 cut(s) 65, 451, 469, 496
Eco130I CCWWGG 1 cut(s) 755
Eco147I AGGCCT 1 cut(s) 751
Eco24I GRGCYC 1 cut(s) 785
Eco57I CTGAAG 1 cut(s) 399
Eco81I CCTNAGG 1 cut(s) 615
EcoRII CCWGG 2 cut(s) 303, 464
EcoT14I CCWWGG 1 cut(s) 755
EcoT38I GRGCYC 1 cut(s) 785
ErhI CCWWGG 1 cut(s) 755
FaeI CATG 4 cut(s) 163, 407, 446, 601
FaqI GGGAC 1 cut(s) 392
FatI CATG 4 cut(s) 159, 403, 442, 597
FauI CCCGC 1 cut(s) 755
Fnu4HI GCNGC 2 cut(s) 270, 426
FokI GGATG 2 cut(s) 17, 396
FriOI GRGCYC 1 cut(s) 785
Fsp4HI GCNGC 2 cut(s) 270, 426
FspBI CTAG 3 cut(s) 219, 366, 786
GlaI GCGC 2 cut(s) 554, 761
GluI GCNGC 2 cut(s) 270, 426
GsaI CCCAGC 2 cut(s) 674, 743
HaeIII GGCC 2 cut(s) 751, 778
HapII CCGG 3 cut(s) 779, 796, 810
HhaI GCGC 2 cut(s) 555, 762
Hin1I GRCGYC 1 cut(s) 790
Hin1II CATG 4 cut(s) 163, 407, 446, 601
Hin6I GCGC 2 cut(s) 553, 760
HinP1I GCGC 2 cut(s) 553, 760
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 2 cut(s) 37, 827
HpaII CCGG 3 cut(s) 779, 796, 810
HphI GGTGA 3 cut(s) 651, 708, 785
Hpy166II GTNNAC 3 cut(s) 137, 246, 733
Hpy188I TCNGA 4 cut(s) 45, 111, 346, 570
Hpy188III TCNNGA 3 cut(s) 219, 377, 786
Hpy8I GTNNAC 3 cut(s) 137, 246, 733
HpyAV CCTTC 2 cut(s) 320, 374
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4IV ACGT 2 cut(s) 372, 790
HpyCH4V TGCA 3 cut(s) 159, 203, 212
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 266, 757
HpyF3I CTNAG 5 cut(s) 255, 345, 395, 615, 744
HpySE526I ACGT 2 cut(s) 372, 790
Hsp92I GRCGYC 1 cut(s) 790
Hsp92II CATG 4 cut(s) 163, 407, 446, 601
HspAI GCGC 2 cut(s) 553, 760
Kzo9I GATC 4 cut(s) 65, 451, 469, 496
LmnI GCTCC 3 cut(s) 400, 592, 607
Lsp1109I GCAGC 1 cut(s) 256
MaeI CTAG 3 cut(s) 219, 366, 786
MaeII ACGT 2 cut(s) 372, 790
MaeIII GTNAC 4 cut(s) 127, 368, 680, 791
MalI GATC 4 cut(s) 67, 453, 471, 498
MboI GATC 4 cut(s) 65, 451, 469, 496
MboII GAAGA 2 cut(s) 32, 602
MhlI GDGCHC 2 cut(s) 597, 785
MluCI AATT 5 cut(s) 123, 213, 311, 474, 556
MlyI GAGTC 1 cut(s) 46
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 3 cut(s) 677, 696, 835
MslI CAYNNNNRTG 2 cut(s) 144, 582
MspI CCGG 3 cut(s) 779, 796, 810
MspR9I CCNGG 3 cut(s) 305, 466, 780
Mva1269I GAATGC 1 cut(s) 205
MvaI CCWGG 2 cut(s) 305, 466
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 3 cut(s) 209, 266, 757
NciI CCSGG 1 cut(s) 780
NdeII GATC 4 cut(s) 65, 451, 469, 496
NlaIII CATG 4 cut(s) 163, 407, 446, 601
NlaIV GGNNCC 2 cut(s) 440, 603
NmuCI GTSAC 2 cut(s) 368, 791
NspI RCATGY 1 cut(s) 407
PceI AGGCCT 1 cut(s) 751
PctI GAATGC 1 cut(s) 205
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 1 cut(s) 827
PkrI GCNGC 2 cut(s) 271, 427
PleI GAGTC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 45
PsiI TTATAA 1 cut(s) 228
Psp6I CCWGG 2 cut(s) 303, 464
PspFI CCCAGC 2 cut(s) 670, 739
PspGI CCWGG 2 cut(s) 303, 464
PspN4I GGNNCC 2 cut(s) 440, 603
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
RseI CAYNNNNRTG 2 cut(s) 144, 582
SaqAI TTAA 3 cut(s) 677, 696, 835
SatI GCNGC 2 cut(s) 270, 426
Sau3AI GATC 4 cut(s) 65, 451, 469, 496
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 3 cut(s) 305, 466, 780
SduI GDGCHC 2 cut(s) 597, 785
SfcI CTRYAG 1 cut(s) 13
SmiMI CAYNNNNRTG 2 cut(s) 144, 582
SpeI ACTAGT 1 cut(s) 365
Sse9I AATT 5 cut(s) 123, 213, 311, 474, 556
SseBI AGGCCT 1 cut(s) 751
SsiI CCGC 4 cut(s) 426, 635, 689, 762
SspMI CTAG 3 cut(s) 219, 366, 786
StuI AGGCCT 1 cut(s) 751
StyD4I CCNGG 3 cut(s) 303, 464, 778
StyI CCWWGG 1 cut(s) 755
TaaI ACNGT 1 cut(s) 545
TaiI ACGT 2 cut(s) 375, 793
TasI AATT 5 cut(s) 123, 213, 311, 474, 556
TatI WGTACW 1 cut(s) 81
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 1 cut(s) 827
Tru1I TTAA 3 cut(s) 677, 696, 835
Tru9I TTAA 3 cut(s) 677, 696, 835
TseFI GTSAC 2 cut(s) 368, 791
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 2 cut(s) 368, 791
TspDTI ATGAA 3 cut(s) 21, 176, 459
TspGWI ACGGA 1 cut(s) 263
XapI RAATTY 1 cut(s) 311
XbaI TCTAGA 2 cut(s) 218, 785
XceI RCATGY 1 cut(s) 407
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 3 cut(s) 219, 366, 786
ZraI GACGTC 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.