MD05G1357600.v1.1

Long-chain-alcohol O-fatty-acyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015566
Physical Location & Seq
Reverse (-)
47328715 .. 47329845
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1357600.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGTTCACAGAAATGATGAAATTGGAAGGAGAAATCCCAAACTTTATCATGGTTTGGATTTTGGTTGTGGCATCACTATCCTACTGTCACACCGTCGGCAACCTCACTTCTCCCGGCACCACCAGACTCCTCGCCATCCTCCCCGTCGTATTCTTCTTCTTCTACCTCCCTCTCAATCTCACCACCATTTTTCTCGGCGGCCCATCGTCTTTCTTCCTCGCTTGGCTTGCCAACTTCAAGCTCCTCCTCTTTGCCTTTAACAAAGGCCCCCTCGCCACCACCCCTCCCACTTCCATCTATCATTTCATCCCCTTAGCTTGCCTTCCAATCAAATTCCGAAACGACCCCAAAAACCCTGAAAAGAAGACGGAACGTCCAGCGCAAAGAATTTCTAATTCAAAACCTCTCCCAAAAGGCAAGAAGTCGACTCTCAACTACGTGATCAAGGCTTTGATTTTTGCAACGTCAGTACAAGTGTACCACAAAAAACAGTTTATTCACCCTAAAATCTTACTCTTCCTCTACTCCATTTACATGTACACCGGCCTTGAACTTATCCTCGCCTTGGTCGCATCCCTGGCCCGAGTCTTCCTTGGCGTTGATTTCGAGCCGCAGTTCGACGAGCCCTACCTCGCCACCTCACTCCAGGACTTCTGGGGACGCAGATGGAACATCATGGTGAGCAAGATCCTCCACCCGACGGTATACGAACCCGTCCGGAAAATCTCCACCCGTGCCCTAGGGAAAAAGCTGGCCCCACTACCGGCAGTAATCGCGTCGTTTTTCGTATCGGCGGTGATGCATGAGCTCATCTTCTATTACATTGGACGGACGTGGCCCACGTGGGAGCTGACGTGCTTCTTCCTCCTCCACGGAATATGTGTGGCCGTTGAGATTGTGATCAAGAAGGCTTTTGACGGTAAGTGGCGGTTGCCTGCCGCTGTTTCGGGGCCATTGGCGATGGCATTTGTGGTGGCGACAGGGGTATGGCTGTTTTTGCCGTCTCTTATGGTGCGGTGTGAAGCGGACGTCATGGCGTACAGAGAATATACTGCATTCACTGAGTTTATGAAAGGTGTTAGTAACTTTGTTAAAGTTAATTCTTCTTTCTTGATCGTAGCTGGAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

377

Amino Acids

42.59

Weight (kDa)

9.64

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT PF03062 187 - 306 1.2e-06 MBOAT, membrane-bound O-acyltransferase family
MBOAT_2 PF13813 203 - 289 1.3e-19 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015566)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01000
malus_domestica MD05G1357600.v1.1 MD10G1331500.v1.1
prunus_persica Prupe.4G010600_v2.0.a1 Prupe.4G010700_v2.0.a1
pyrus_communis pycom05g32350
rosa_chinensis RchiOBHm_Chr5g0001531
rosa_laevigata RLG00000030936
rosa_multiflora Rmu_sc0003749.1_g000005 Rmu_sc0022840.1_g000001
rosa_roxburghii Rroxscaffold_1G00074870
rosa_rugosa Rorug04G0390900
rosa_samantha Rh5AG012000 Rh5CG013000 Rh5DG012300
rosa_wichuraiana Rw5G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1032
AccB1I GGYRCC 1 cut(s) 116
AccI GTMKAC 2 cut(s) 425, 705
AccII CGCG 1 cut(s) 776
AccIII TCCGGA 1 cut(s) 717
AciI CCGC 7 cut(s) 198, 611, 794, 928, 939, 1015, 1025
AclWI GGATC 1 cut(s) 682
AcoI YGGCCR 1 cut(s) 885
AcsI RAATTY 2 cut(s) 332, 387
AcvI CACGTG 1 cut(s) 843
AcyI GRCGYC 1 cut(s) 1029
AfaI GTAC 4 cut(s) 471, 479, 539, 1040
AfiI CCNNNNNNNGG 3 cut(s) 565, 700, 763
AflIII ACRYGT 1 cut(s) 534
AgsI TTSAA 3 cut(s) 238, 399, 551
AhdI GACNNNNNGTC 1 cut(s) 372
AjiI CACGTC 2 cut(s) 834, 855
AjnI CCWGG 2 cut(s) 576, 645
AluBI AGCT 6 cut(s) 241, 317, 751, 808, 850, 1121
AluI AGCT 6 cut(s) 241, 317, 751, 808, 850, 1121
Alw21I GWGCWC 1 cut(s) 810
Alw26I GTCTC 1 cut(s) 1008
AlwI GGATC 1 cut(s) 682
Ama87I CYCGRG 1 cut(s) 582
Aor13HI TCCGGA 1 cut(s) 717
AoxI GGCC 8 cut(s) 199, 265, 544, 579, 753, 836, 885, 950
ApoI RAATTY 2 cut(s) 332, 387
AspA2I CCTAGG 1 cut(s) 739
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 6 cut(s) 200, 266, 580, 754, 837, 950
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 4 cut(s) 172, 491, 691, 808
AvaI CYCGRG 1 cut(s) 582
AvrII CCTAGG 1 cut(s) 739
BaeGI GKGCMC 1 cut(s) 739
BaeI ACNNNNGTAYC 2 cut(s) 461, 494
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 2 cut(s) 627, 810
BbrPI CACGTG 1 cut(s) 843
BbsI GAAGAC 2 cut(s) 371, 580
Bbv12I GWGCWC 1 cut(s) 810
BccI CCATC 5 cut(s) 143, 211, 302, 660, 955
BceAI ACGGC 2 cut(s) 872, 985
BciT130I CCWGG 2 cut(s) 578, 647
BclI TGATCA 2 cut(s) 441, 900
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 1 cut(s) 1008
BfaI CTAG 1 cut(s) 740
BisI GCNGC 3 cut(s) 199, 611, 939
BlnI CCTAGG 1 cut(s) 739
BlsI GCNGC 3 cut(s) 200, 612, 940
Bme1390I CCNGG 3 cut(s) 114, 578, 647
BmeRI GACNNNNNGTC 1 cut(s) 372
BmeT110I CYCGRG 1 cut(s) 582
BmgBI CACGTC 2 cut(s) 834, 855
BmgT120I GGNCC 6 cut(s) 200, 266, 580, 754, 837, 950
BmiI GGNNCC 4 cut(s) 118, 268, 756, 951
BmrFI CCNGG 3 cut(s) 114, 578, 647
BmsI GCATC 3 cut(s) 80, 581, 789
BpiI GAAGAC 2 cut(s) 371, 580
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 313
BpuMI CCSGG 1 cut(s) 114
BsaAI YACGTR 2 cut(s) 439, 843
BsaBI GATNNNNATC 1 cut(s) 899
BsaHI GRCGYC 1 cut(s) 1029
BsaJI CCNNGG 5 cut(s) 564, 576, 592, 739, 871
BsaWI WCCGGW 1 cut(s) 717
BsaXI ACNNNNNCTCC 4 cut(s) 268, 298, 839, 869
Bsc4I CCNNNNNNNGG 3 cut(s) 565, 700, 763
Bse118I RCCGGY 2 cut(s) 542, 763
Bse8I GATNNNNATC 1 cut(s) 899
BseAI TCCGGA 1 cut(s) 717
BseBI CCWGG 2 cut(s) 578, 647
BseDI CCNNGG 5 cut(s) 564, 576, 592, 739, 871
BseGI GGATG 3 cut(s) 135, 306, 572
BseJI GATNNNNATC 1 cut(s) 899
BseLI CCNNNNNNNGG 3 cut(s) 565, 700, 763
BseMII CTCAG 1 cut(s) 1053
BseRI GAGGAG 4 cut(s) 119, 233, 236, 857
BseSI GKGCMC 1 cut(s) 739
Bsh1236I CGCG 1 cut(s) 776
BshFI GGCC 8 cut(s) 201, 267, 546, 581, 755, 838, 887, 952
BshNI GGYRCC 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 810
BsiHKCI CYCGRG 1 cut(s) 582
BsiSI CCGG 4 cut(s) 114, 543, 718, 764
BslFI GGGAC 1 cut(s) 672
BslI CCNNNNNNNGG 3 cut(s) 565, 700, 763
BsmAI GTCTC 1 cut(s) 1008
BsmBI CGTCTC 1 cut(s) 1008
BsmFI GGGAC 1 cut(s) 672
BsmI GAATGC 1 cut(s) 1055
BsnI GGCC 8 cut(s) 201, 267, 546, 581, 755, 838, 887, 952
BsoBI CYCGRG 1 cut(s) 582
Bsp1286I GDGCHC 3 cut(s) 627, 739, 810
Bsp13I TCCGGA 1 cut(s) 717
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 4 cut(s) 441, 687, 900, 1113
BspACI CCGC 7 cut(s) 198, 611, 794, 928, 939, 1015, 1025
BspANI GGCC 8 cut(s) 201, 267, 546, 581, 755, 838, 887, 952
BspCNI CTCAG 1 cut(s) 1054
BspEI TCCGGA 1 cut(s) 717
BspFNI CGCG 1 cut(s) 776
BspLI GGNNCC 4 cut(s) 118, 268, 756, 951
BspPI GGATC 1 cut(s) 682
BspT107I GGYRCC 1 cut(s) 116
BsrFI RCCGGY 2 cut(s) 542, 763
BsrGI TGTACA 1 cut(s) 537
BssAI RCCGGY 2 cut(s) 542, 763
BssECI CCNNGG 5 cut(s) 564, 576, 592, 739, 871
BssMI GATC 4 cut(s) 441, 687, 900, 1113
BssNAI GTATAC 1 cut(s) 706
BssNI GRCGYC 1 cut(s) 1029
BssT1I CCWWGG 3 cut(s) 564, 592, 739
Bst1107I GTATAC 1 cut(s) 706
Bst2UI CCWGG 2 cut(s) 578, 647
Bst4CI ACNGT 5 cut(s) 86, 94, 492, 703, 920
Bst6I CTCTTC 1 cut(s) 521
BstACI GRCGYC 1 cut(s) 1029
BstAUI TGTACA 1 cut(s) 537
BstBAI YACGTR 2 cut(s) 439, 843
BstC8I GCNNGC 4 cut(s) 228, 319, 753, 936
BstDEI CTNAG 2 cut(s) 313, 1062
BstDSI CCRYGG 1 cut(s) 871
BstF5I GGATG 3 cut(s) 135, 306, 572
BstFNI CGCG 1 cut(s) 776
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 4 cut(s) 444, 690, 903, 1116
BstMAI GTCTC 1 cut(s) 1008
BstMBI GATC 4 cut(s) 441, 687, 900, 1113
BstMWI GCNNNNNNNGC 5 cut(s) 227, 569, 578, 773, 997
BstNI CCWGG 2 cut(s) 578, 647
BstNSI RCATGY 1 cut(s) 538
BstSCI CCNGG 3 cut(s) 112, 576, 645
BstSLI GKGCMC 1 cut(s) 739
BstUI CGCG 1 cut(s) 776
BstV2I GAAGAC 2 cut(s) 371, 580
BstX2I RGATCY 1 cut(s) 687
BstYI RGATCY 1 cut(s) 687
BstZ17I GTATAC 1 cut(s) 706
BsuRI GGCC 8 cut(s) 201, 267, 546, 581, 755, 838, 887, 952
BtgI CCRYGG 1 cut(s) 871
BtgZI GCGATG 1 cut(s) 974
BtrI CACGTC 2 cut(s) 834, 855
BtsCI GGATG 3 cut(s) 135, 306, 572
BtsIMutI CAGTG 1 cut(s) 1059
Cac8I GCNNGC 4 cut(s) 228, 319, 753, 936
CfoI GCGC 1 cut(s) 382
Cfr10I RCCGGY 2 cut(s) 542, 763
Cfr13I GGNCC 6 cut(s) 200, 266, 580, 754, 837, 950
CseI GACGC 2 cut(s) 669, 765
Csp6I GTAC 4 cut(s) 470, 478, 538, 1039
CviAII CATG 5 cut(s) 49, 535, 676, 803, 1033
CviQI GTAC 4 cut(s) 470, 478, 538, 1039
DdeI CTNAG 2 cut(s) 313, 1062
DpnI GATC 4 cut(s) 443, 689, 902, 1115
DpnII GATC 4 cut(s) 441, 687, 900, 1113
DriI GACNNNNNGTC 1 cut(s) 372
EaeI YGGCCR 1 cut(s) 885
Eam1104I CTCTTC 1 cut(s) 521
Eam1105I GACNNNNNGTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 521
Ecl136II GAGCTC 1 cut(s) 808
Eco130I CCWWGG 3 cut(s) 564, 592, 739
Eco24I GRGCYC 2 cut(s) 627, 810
Eco53kI GAGCTC 1 cut(s) 808
Eco72I CACGTG 1 cut(s) 843
Eco88I CYCGRG 1 cut(s) 582
EcoICRI GAGCTC 1 cut(s) 808
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 576, 645
EcoT14I CCWWGG 3 cut(s) 564, 592, 739
EcoT22I ATGCAT 1 cut(s) 804
EcoT38I GRGCYC 2 cut(s) 627, 810
ErhI CCWWGG 3 cut(s) 564, 592, 739
Esp3I CGTCTC 1 cut(s) 1008
FaeI CATG 5 cut(s) 52, 538, 679, 806, 1036
FaqI GGGAC 1 cut(s) 672
FatI CATG 5 cut(s) 48, 534, 675, 802, 1032
FbaI TGATCA 2 cut(s) 441, 900
FblI GTMKAC 2 cut(s) 425, 705
Fnu4HI GCNGC 3 cut(s) 199, 611, 939
FokI GGATG 3 cut(s) 122, 293, 559
FriOI GRGCYC 2 cut(s) 627, 810
Fsp4HI GCNGC 3 cut(s) 199, 611, 939
FspBI CTAG 1 cut(s) 740
GlaI GCGC 1 cut(s) 381
GluI GCNGC 3 cut(s) 199, 611, 939
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 8 cut(s) 201, 267, 546, 581, 755, 838, 887, 952
HapII CCGG 4 cut(s) 114, 543, 718, 764
HgaI GACGC 2 cut(s) 669, 765
HhaI GCGC 1 cut(s) 382
Hin1I GRCGYC 1 cut(s) 1029
Hin1II CATG 5 cut(s) 52, 538, 679, 806, 1036
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HinfI GANTC 3 cut(s) 126, 427, 585
HpaII CCGG 4 cut(s) 114, 543, 718, 764
HphI GGTGA 4 cut(s) 172, 491, 691, 808
Hpy166II GTNNAC 5 cut(s) 6, 426, 478, 540, 706
Hpy188I TCNGA 1 cut(s) 338
Hpy188III TCNNGA 3 cut(s) 718, 904, 1111
Hpy8I GTNNAC 5 cut(s) 6, 426, 478, 540, 706
Hpy99I CGWCG 5 cut(s) 98, 149, 623, 703, 781
HpyAV CCTTC 3 cut(s) 20, 332, 901
HpyCH4III ACNGT 5 cut(s) 86, 94, 492, 703, 920
HpyCH4IV ACGT 7 cut(s) 373, 438, 464, 833, 842, 854, 1029
HpyCH4V TGCA 3 cut(s) 461, 802, 1055
HpyF10VI GCNNNNNNNGC 5 cut(s) 227, 569, 578, 773, 997
HpyF3I CTNAG 2 cut(s) 313, 1062
HpySE526I ACGT 7 cut(s) 373, 438, 464, 833, 842, 854, 1029
Hsp92I GRCGYC 1 cut(s) 1029
Hsp92II CATG 5 cut(s) 52, 538, 679, 806, 1036
HspAI GCGC 1 cut(s) 380
Kpn2I TCCGGA 1 cut(s) 717
Ksp22I TGATCA 2 cut(s) 441, 900
Kzo9I GATC 4 cut(s) 441, 687, 900, 1113
LmnI GCTCC 2 cut(s) 246, 847
LweI GCATC 3 cut(s) 80, 581, 789
MaeI CTAG 1 cut(s) 740
MaeII ACGT 7 cut(s) 373, 438, 464, 833, 842, 854, 1029
MaeIII GTNAC 2 cut(s) 86, 1082
MalI GATC 4 cut(s) 443, 689, 902, 1115
MboI GATC 4 cut(s) 441, 687, 900, 1113
MflI RGATCY 1 cut(s) 687
MhlI GDGCHC 3 cut(s) 627, 739, 810
MluCI AATT 5 cut(s) 20, 332, 387, 394, 1099
MlyI GAGTC 3 cut(s) 120, 421, 594
Mph1103I ATGCAT 1 cut(s) 804
MroI TCCGGA 1 cut(s) 717
MseI TTAA 3 cut(s) 258, 1092, 1098
MslI CAYNNNNRTG 3 cut(s) 11, 533, 677
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 4 cut(s) 114, 543, 718, 764
MspR9I CCNGG 3 cut(s) 114, 578, 647
Mva1269I GAATGC 1 cut(s) 1055
MvaI CCWGG 2 cut(s) 578, 647
MvnI CGCG 1 cut(s) 776
MwoI GCNNNNNNNGC 5 cut(s) 227, 569, 578, 773, 997
NciI CCSGG 1 cut(s) 114
NdeII GATC 4 cut(s) 441, 687, 900, 1113
NlaIII CATG 5 cut(s) 52, 538, 679, 806, 1036
NlaIV GGNNCC 4 cut(s) 118, 268, 756, 951
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 1 cut(s) 86
NsiI ATGCAT 1 cut(s) 804
NspI RCATGY 1 cut(s) 538
PciI ACATGT 1 cut(s) 534
PcsI WCGNNNNNNNCGW 1 cut(s) 839
PctI GAATGC 1 cut(s) 1055
PfoI TCCNGGA 1 cut(s) 645
PkrI GCNGC 3 cut(s) 200, 612, 940
PleI GAGTC 3 cut(s) 120, 421, 593
PmaCI CACGTG 1 cut(s) 843
PmlI CACGTG 1 cut(s) 843
PpsI GAGTC 3 cut(s) 120, 421, 593
Ppu21I YACGTR 2 cut(s) 439, 843
PscI ACATGT 1 cut(s) 534
Psp124BI GAGCTC 1 cut(s) 810
Psp6I CCWGG 2 cut(s) 576, 645
PspCI CACGTG 1 cut(s) 843
PspGI CCWGG 2 cut(s) 576, 645
PspN4I GGNNCC 4 cut(s) 118, 268, 756, 951
PspPI GGNCC 6 cut(s) 200, 266, 580, 754, 837, 950
PsuI RGATCY 1 cut(s) 687
RsaI GTAC 4 cut(s) 471, 479, 539, 1040
RsaNI GTAC 4 cut(s) 470, 478, 538, 1039
RseI CAYNNNNRTG 3 cut(s) 11, 533, 677
SacI GAGCTC 1 cut(s) 810
SalI GTCGAC 1 cut(s) 424
SaqAI TTAA 3 cut(s) 258, 1092, 1098
SatI GCNGC 3 cut(s) 199, 611, 939
Sau3AI GATC 4 cut(s) 441, 687, 900, 1113
Sau96I GGNCC 6 cut(s) 200, 266, 580, 754, 837, 950
SchI GAGTC 3 cut(s) 120, 421, 594
ScrFI CCNGG 3 cut(s) 114, 578, 647
SduI GDGCHC 3 cut(s) 627, 739, 810
SfaNI GCATC 3 cut(s) 80, 581, 789
SmiMI CAYNNNNRTG 3 cut(s) 11, 533, 677
Sse9I AATT 5 cut(s) 20, 332, 387, 394, 1099
SsiI CCGC 7 cut(s) 198, 611, 794, 928, 939, 1015, 1025
SspMI CTAG 1 cut(s) 740
SstI GAGCTC 1 cut(s) 810
StyD4I CCNGG 3 cut(s) 112, 576, 645
StyI CCWWGG 3 cut(s) 564, 592, 739
TaaI ACNGT 5 cut(s) 86, 94, 492, 703, 920
TaiI ACGT 7 cut(s) 376, 441, 467, 836, 845, 857, 1032
TaqI TCGA 3 cut(s) 425, 606, 618
TasI AATT 5 cut(s) 20, 332, 387, 394, 1099
TatI WGTACW 2 cut(s) 469, 537
TauI GCSGC 3 cut(s) 201, 613, 941
Tru1I TTAA 3 cut(s) 258, 1092, 1098
Tru9I TTAA 3 cut(s) 258, 1092, 1098
TscAI CASTG 1 cut(s) 1066
TseFI GTSAC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 3 cut(s) 32, 295, 1085
TspGWI ACGGA 3 cut(s) 383, 844, 888
TspRI CASTG 1 cut(s) 1066
XapI RAATTY 2 cut(s) 332, 387
XceI RCATGY 1 cut(s) 538
XmaJI CCTAGG 1 cut(s) 739
XmiI GTMKAC 2 cut(s) 425, 705
XspI CTAG 1 cut(s) 740
ZraI GACGTC 1 cut(s) 1030
Zsp2I ATGCAT 1 cut(s) 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.