Rroxscaffold_1G00074870

Long-chain-alcohol O-fatty-acyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015566
Physical Location & Seq
Reverse (-)
95693264 .. 95694862
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074870.1

Sequence Viewer

Length: 963 bp
ATGCGAAACAGCTCCCTCTCCACTCATTCACCTCAAAACGTCTCTGAAACTCTCTCACAGAAAACAATGGAAGGAGAGATCCCCAACTTCGTTATGGTGTGGACCTTAGTCGTCGCATCCCTAACCTACTGCCACACCATCGGAAACCTAACTTCTCCGGGAACCACCAGACTTCTCTTAGTACTTCCTGTATTCTTCTTCTTCTTCTATCTCCCTCTTCACCTCACCACCATCTTCCTCGGCGGCCCCTCCTCCTTCTTTCTCTGTTGGCTCGCAAACTTCAAGCTCCTCCTCTTCGCTTTCGGCAAAGGCCCCTTATCGTCTGACCCGCCCCTCGCTCATTTCATCCCCTTGGCTTGTCTCCCCATCAAGTTCCGAACAGATTCCGAAAAAGCTCAAAATCTAAAGAAAGGCCAGAAGTCCACTCTCAACTACGCCACAAAGGCTCTGATATTCGCTACTTCGGTTCAAGTTTTCCACAACAAGGAGTCCATCCACCCCAAGATCCTAATGTTTCTTTACGCCATTTACATGTACACCGGTTTAGAGCTCGTCCTGGCCTTTGTCGCCGCTGGAGCTCGAGTCTTTTTGGGTGTCGACTTCGAGCCGCAGTTTGACGAGCCCTACCTCGCCACCTCCCTACAGGACTTCTGGGGGAGGAGGTGGAACATCATGGTCAACAGAATCCTGCACCCCACCGTGTATGAACCAGTCCGGAACATTTCCAGCCGTTGGATCGGGAAAGAGTGGGCCCCTCTGCCAGCAGTGCTCGCGTCCTTTCTCGTTTCTGCGGTGATGCATGAGTTCGTATTCTATTACATTGGTCGGACCAAGCCCACATGGGATCTCACGTGCTTCTTTCTCCTCCATGGTGTGTGTTTGTCTATTGAGATTATTGTTAAGAAGGCGTTTAGGGGAGTAAGTGGAGAATGCCGGCCGCCGTGTCGGGGCCGCTGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

320

Amino Acids

36.38

Weight (kDa)

9.4

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT PF03062 170 - 299 3.2e-06 MBOAT, membrane-bound O-acyltransferase family
MBOAT_2 PF13813 202 - 288 1.2e-19 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015566)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01000
malus_domestica MD05G1357600.v1.1 MD10G1331500.v1.1
prunus_persica Prupe.4G010600_v2.0.a1 Prupe.4G010700_v2.0.a1
pyrus_communis pycom05g32350
rosa_chinensis RchiOBHm_Chr5g0001531
rosa_laevigata RLG00000030936
rosa_multiflora Rmu_sc0003749.1_g000005 Rmu_sc0022840.1_g000001
rosa_roxburghii Rroxscaffold_1G00074870
rosa_rugosa Rorug04G0390900
rosa_samantha Rh5AG012000 Rh5CG013000 Rh5DG012300
rosa_wichuraiana Rw5G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 732
AccI GTMKAC 1 cut(s) 597
AccII CGCG 1 cut(s) 773
AccIII TCCGGA 1 cut(s) 714
AciI CCGC 7 cut(s) 243, 329, 570, 608, 791, 938, 952
AclWI GGATC 4 cut(s) 73, 499, 743, 852
AcoI YGGCCR 1 cut(s) 935
AcvI CACGTG 1 cut(s) 852
AfaI GTAC 2 cut(s) 183, 536
AfiI CCNNNNNNNGG 3 cut(s) 484, 732, 947
AflIII ACRYGT 1 cut(s) 531
AgeI ACCGGT 1 cut(s) 539
AgsI TTSAA 2 cut(s) 283, 470
AjnI CCWGG 1 cut(s) 555
AluBI AGCT 5 cut(s) 12, 286, 395, 550, 578
AluI AGCT 5 cut(s) 12, 286, 395, 550, 578
Alw21I GWGCWC 3 cut(s) 552, 580, 771
Alw26I GTCTC 2 cut(s) 46, 365
AlwI GGATC 4 cut(s) 73, 499, 743, 852
Ama87I CYCGRG 1 cut(s) 579
Aor13HI TCCGGA 1 cut(s) 714
AoxI GGCC 7 cut(s) 244, 310, 412, 558, 750, 935, 949
ApaI GGGCCC 1 cut(s) 754
AsiGI ACCGGT 1 cut(s) 539
Asp700I GAANNNNTTC 1 cut(s) 382
AspS9I GGNCC 7 cut(s) 102, 245, 311, 750, 751, 828, 949
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 4 cut(s) 21, 212, 217, 805
AvaI CYCGRG 1 cut(s) 579
AvaII GGWCC 2 cut(s) 102, 828
BaeGI GKGCMC 1 cut(s) 754
BanII GRGCYC 4 cut(s) 552, 580, 624, 754
BbrPI CACGTG 1 cut(s) 852
Bbv12I GWGCWC 3 cut(s) 552, 580, 771
BccI CCATC 4 cut(s) 146, 239, 374, 500
BceAI ACGGC 2 cut(s) 714, 925
BciT130I CCWGG 1 cut(s) 557
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 2 cut(s) 46, 365
BfmI CTRYAG 1 cut(s) 641
BglI GCCNNNNNGGC 1 cut(s) 443
BisI GCNGC 5 cut(s) 244, 570, 608, 938, 952
BlsI GCNGC 5 cut(s) 245, 571, 609, 939, 953
BmcAI AGTACT 1 cut(s) 183
Bme1390I CCNGG 2 cut(s) 159, 557
Bme18I GGWCC 2 cut(s) 102, 828
BmeT110I CYCGRG 1 cut(s) 579
BmgT120I GGNCC 7 cut(s) 102, 245, 311, 750, 751, 828, 949
BmiI GGNNCC 6 cut(s) 163, 247, 313, 752, 753, 950
BmrFI CCNGG 2 cut(s) 159, 557
BmsI GCATC 2 cut(s) 125, 786
BoxI GACNNNNGTC 1 cut(s) 107
BpmI CTGGAG 1 cut(s) 594
BpuMI CCSGG 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 852
BsaJI CCNNGG 3 cut(s) 238, 351, 868
BsaWI WCCGGW 2 cut(s) 539, 714
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bsc4I CCNNNNNNNGG 3 cut(s) 484, 732, 947
Bse118I RCCGGY 2 cut(s) 539, 933
Bse1I ACTGG 1 cut(s) 710
BseAI TCCGGA 1 cut(s) 714
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 3 cut(s) 238, 351, 868
BseGI GGATG 3 cut(s) 116, 345, 492
BseLI CCNNNNNNNGG 3 cut(s) 484, 732, 947
BseNI ACTGG 1 cut(s) 710
BseRI GAGGAG 5 cut(s) 241, 278, 281, 673, 854
BseSI GKGCMC 1 cut(s) 754
BseX3I CGGCCG 1 cut(s) 935
BsgI GTGCAG 1 cut(s) 674
Bsh1236I CGCG 1 cut(s) 773
Bsh1285I CGRYCG 1 cut(s) 938
BshFI GGCC 7 cut(s) 246, 312, 414, 560, 752, 937, 951
BshTI ACCGGT 1 cut(s) 539
BsiEI CGRYCG 1 cut(s) 938
BsiHKAI GWGCWC 3 cut(s) 552, 580, 771
BsiHKCI CYCGRG 1 cut(s) 579
BsiSI CCGG 4 cut(s) 158, 540, 715, 934
BslI CCNNNNNNNGG 3 cut(s) 484, 732, 947
BsmAI GTCTC 2 cut(s) 46, 365
BsmBI CGTCTC 1 cut(s) 46
BsmI GAATGC 1 cut(s) 935
BsnI GGCC 7 cut(s) 246, 312, 414, 560, 752, 937, 951
BsoBI CYCGRG 1 cut(s) 579
Bsp120I GGGCCC 1 cut(s) 750
Bsp1286I GDGCHC 5 cut(s) 552, 580, 624, 754, 771
Bsp13I TCCGGA 1 cut(s) 714
Bsp1407I TGTACA 1 cut(s) 534
Bsp143I GATC 4 cut(s) 78, 504, 735, 844
Bsp19I CCATGG 1 cut(s) 868
BspACI CCGC 7 cut(s) 243, 329, 570, 608, 791, 938, 952
BspANI GGCC 7 cut(s) 246, 312, 414, 560, 752, 937, 951
BspEI TCCGGA 1 cut(s) 714
BspFNI CGCG 1 cut(s) 773
BspLI GGNNCC 6 cut(s) 163, 247, 313, 752, 753, 950
BspPI GGATC 4 cut(s) 73, 499, 743, 852
BsrFI RCCGGY 2 cut(s) 539, 933
BsrGI TGTACA 1 cut(s) 534
BsrI ACTGG 1 cut(s) 710
BssAI RCCGGY 2 cut(s) 539, 933
BssECI CCNNGG 3 cut(s) 238, 351, 868
BssMI GATC 4 cut(s) 78, 504, 735, 844
BssT1I CCWWGG 2 cut(s) 351, 868
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 1 cut(s) 700
Bst6I CTCTTC 2 cut(s) 222, 299
BstAUI TGTACA 1 cut(s) 534
BstBAI YACGTR 1 cut(s) 852
BstC8I GCNNGC 5 cut(s) 273, 762, 771, 935, 956
BstDEI CTNAG 2 cut(s) 106, 178
BstDSI CCRYGG 1 cut(s) 868
BstF5I GGATG 3 cut(s) 116, 345, 492
BstFNI CGCG 1 cut(s) 773
BstKTI GATC 4 cut(s) 81, 507, 738, 847
BstMAI GTCTC 2 cut(s) 46, 365
BstMBI GATC 4 cut(s) 78, 504, 735, 844
BstMCI CGRYCG 1 cut(s) 938
BstMWI GCNNNNNNNGC 5 cut(s) 443, 566, 575, 766, 770
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 1 cut(s) 535
BstPAI GACNNNNGTC 1 cut(s) 107
BstSCI CCNGG 2 cut(s) 157, 555
BstSFI CTRYAG 1 cut(s) 641
BstSLI GKGCMC 1 cut(s) 754
BstUI CGCG 1 cut(s) 773
BstX2I RGATCY 3 cut(s) 78, 504, 844
BstYI RGATCY 3 cut(s) 78, 504, 844
BstZI CGGCCG 1 cut(s) 935
BsuRI GGCC 7 cut(s) 246, 312, 414, 560, 752, 937, 951
BtgI CCRYGG 1 cut(s) 868
BtsCI GGATG 3 cut(s) 116, 345, 492
BtsI GCAGTG 1 cut(s) 771
BtsIMutI CAGTG 1 cut(s) 771
Cac8I GCNNGC 5 cut(s) 273, 762, 771, 935, 956
Cfr10I RCCGGY 2 cut(s) 539, 933
Cfr13I GGNCC 7 cut(s) 102, 245, 311, 750, 751, 828, 949
CseI GACGC 1 cut(s) 762
Csp6I GTAC 2 cut(s) 182, 535
CspAI ACCGGT 1 cut(s) 539
CviAII CATG 5 cut(s) 532, 673, 800, 840, 869
CviQI GTAC 2 cut(s) 182, 535
DdeI CTNAG 2 cut(s) 106, 178
DpnI GATC 4 cut(s) 80, 506, 737, 846
DpnII GATC 4 cut(s) 78, 504, 735, 844
EaeI YGGCCR 1 cut(s) 935
EagI CGGCCG 1 cut(s) 935
Eam1104I CTCTTC 2 cut(s) 222, 299
EarI CTCTTC 2 cut(s) 222, 299
Ecl136II GAGCTC 2 cut(s) 550, 578
EclXI CGGCCG 1 cut(s) 935
Eco130I CCWWGG 2 cut(s) 351, 868
Eco24I GRGCYC 4 cut(s) 552, 580, 624, 754
Eco47I GGWCC 2 cut(s) 102, 828
Eco52I CGGCCG 1 cut(s) 935
Eco53kI GAGCTC 2 cut(s) 550, 578
Eco72I CACGTG 1 cut(s) 852
Eco88I CYCGRG 1 cut(s) 579
EcoICRI GAGCTC 2 cut(s) 550, 578
EcoO109I RGGNCCY 2 cut(s) 311, 751
EcoRII CCWGG 1 cut(s) 555
EcoT14I CCWWGG 2 cut(s) 351, 868
EcoT22I ATGCAT 1 cut(s) 801
EcoT38I GRGCYC 4 cut(s) 552, 580, 624, 754
ErhI CCWWGG 2 cut(s) 351, 868
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 5 cut(s) 535, 676, 803, 843, 872
FaiI YATR 7 cut(s) 95, 533, 674, 705, 801, 841, 870
FatI CATG 5 cut(s) 531, 672, 799, 839, 868
FauI CCCGC 1 cut(s) 336
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 5 cut(s) 244, 570, 608, 938, 952
FokI GGATG 3 cut(s) 103, 332, 479
FriOI GRGCYC 4 cut(s) 552, 580, 624, 754
Fsp4HI GCNGC 5 cut(s) 244, 570, 608, 938, 952
GluI GCNGC 5 cut(s) 244, 570, 608, 938, 952
GsuI CTGGAG 1 cut(s) 594
HaeIII GGCC 7 cut(s) 246, 312, 414, 560, 752, 937, 951
HapII CCGG 4 cut(s) 158, 540, 715, 934
HgaI GACGC 1 cut(s) 762
Hin1II CATG 5 cut(s) 535, 676, 803, 843, 872
HincII GTYRAC 2 cut(s) 598, 679
HindII GTYRAC 2 cut(s) 598, 679
HinfI GANTC 4 cut(s) 383, 488, 582, 684
HpaII CCGG 4 cut(s) 158, 540, 715, 934
HphI GGTGA 4 cut(s) 21, 212, 217, 805
Hpy166II GTNNAC 5 cut(s) 102, 423, 537, 598, 679
Hpy188I TCNGA 7 cut(s) 46, 143, 325, 377, 388, 450, 828
Hpy188III TCNNGA 2 cut(s) 715, 739
Hpy8I GTNNAC 5 cut(s) 102, 423, 537, 598, 679
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 3 cut(s) 65, 265, 898
HpyCH4III ACNGT 1 cut(s) 700
HpyCH4IV ACGT 2 cut(s) 39, 851
HpyCH4V TGCA 2 cut(s) 691, 799
HpyF10VI GCNNNNNNNGC 5 cut(s) 443, 566, 575, 766, 770
HpyF3I CTNAG 2 cut(s) 106, 178
HpySE526I ACGT 2 cut(s) 39, 851
Hsp92II CATG 5 cut(s) 535, 676, 803, 843, 872
Kpn2I TCCGGA 1 cut(s) 714
KroI GCCGGC 1 cut(s) 933
KroNI GCCGGC 1 cut(s) 935
Kzo9I GATC 4 cut(s) 78, 504, 735, 844
LmnI GCTCC 3 cut(s) 17, 291, 575
LweI GCATC 2 cut(s) 125, 786
MaeII ACGT 2 cut(s) 39, 851
MalI GATC 4 cut(s) 80, 506, 737, 846
MboI GATC 4 cut(s) 78, 504, 735, 844
MboII GAAGA 7 cut(s) 187, 190, 193, 196, 209, 226, 286
MflI RGATCY 3 cut(s) 78, 504, 844
MhlI GDGCHC 5 cut(s) 552, 580, 624, 754, 771
MlyI GAGTC 2 cut(s) 497, 591
MmeI TCCRAC 2 cut(s) 713, 806
Mph1103I ATGCAT 1 cut(s) 801
MroI TCCGGA 1 cut(s) 714
MroNI GCCGGC 1 cut(s) 933
MroXI GAANNNNTTC 1 cut(s) 382
MseI TTAA 1 cut(s) 900
MslI CAYNNNNRTG 1 cut(s) 530
MspA1I CMGCKG 2 cut(s) 572, 954
MspI CCGG 4 cut(s) 158, 540, 715, 934
MspR9I CCNGG 2 cut(s) 159, 557
Mva1269I GAATGC 1 cut(s) 935
MvaI CCWGG 1 cut(s) 557
MvnI CGCG 1 cut(s) 773
MwoI GCNNNNNNNGC 5 cut(s) 443, 566, 575, 766, 770
NaeI GCCGGC 1 cut(s) 935
NciI CCSGG 1 cut(s) 159
NcoI CCATGG 1 cut(s) 868
NdeII GATC 4 cut(s) 78, 504, 735, 844
NgoMIV GCCGGC 1 cut(s) 933
NlaIII CATG 5 cut(s) 535, 676, 803, 843, 872
NlaIV GGNNCC 6 cut(s) 163, 247, 313, 752, 753, 950
NmeAIII GCCGAG 1 cut(s) 219
NsiI ATGCAT 1 cut(s) 801
NspI RCATGY 1 cut(s) 535
PaeR7I CTCGAG 1 cut(s) 579
PciI ACATGT 1 cut(s) 531
PctI GAATGC 1 cut(s) 935
PdiI GCCGGC 1 cut(s) 935
PdmI GAANNNNTTC 1 cut(s) 382
PfeI GAWTC 2 cut(s) 383, 684
PflMI CCANNNNNTGG 1 cut(s) 732
PfoI TCCNGGA 1 cut(s) 157
PinAI ACCGGT 1 cut(s) 539
PkrI GCNGC 5 cut(s) 245, 571, 609, 939, 953
PleI GAGTC 2 cut(s) 496, 590
PmaCI CACGTG 1 cut(s) 852
PmlI CACGTG 1 cut(s) 852
PpsI GAGTC 2 cut(s) 496, 590
Ppu21I YACGTR 1 cut(s) 852
PscI ACATGT 1 cut(s) 531
PshAI GACNNNNGTC 1 cut(s) 107
Psp124BI GAGCTC 2 cut(s) 552, 580
Psp6I CCWGG 1 cut(s) 555
PspCI CACGTG 1 cut(s) 852
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 6 cut(s) 163, 247, 313, 752, 753, 950
PspOMI GGGCCC 1 cut(s) 750
PspPI GGNCC 7 cut(s) 102, 245, 311, 750, 751, 828, 949
PspXI VCTCGAGB 1 cut(s) 579
PsuI RGATCY 3 cut(s) 78, 504, 844
RsaI GTAC 2 cut(s) 183, 536
RsaNI GTAC 2 cut(s) 182, 535
RseI CAYNNNNRTG 1 cut(s) 530
SacI GAGCTC 2 cut(s) 552, 580
SalI GTCGAC 1 cut(s) 596
SaqAI TTAA 1 cut(s) 900
SatI GCNGC 5 cut(s) 244, 570, 608, 938, 952
Sau3AI GATC 4 cut(s) 78, 504, 735, 844
Sau96I GGNCC 7 cut(s) 102, 245, 311, 750, 751, 828, 949
ScaI AGTACT 1 cut(s) 183
SchI GAGTC 2 cut(s) 497, 591
ScrFI CCNGG 2 cut(s) 159, 557
SduI GDGCHC 5 cut(s) 552, 580, 624, 754, 771
SfaNI GCATC 2 cut(s) 125, 786
SfcI CTRYAG 1 cut(s) 641
Sfr274I CTCGAG 1 cut(s) 579
SinI GGWCC 2 cut(s) 102, 828
SlaI CTCGAG 1 cut(s) 579
SmiMI CAYNNNNRTG 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 579
SmoI CTYRAG 1 cut(s) 579
SsiI CCGC 7 cut(s) 243, 329, 570, 608, 791, 938, 952
SstI GAGCTC 2 cut(s) 552, 580
StyD4I CCNGG 2 cut(s) 157, 555
StyI CCWWGG 2 cut(s) 351, 868
TaaI ACNGT 1 cut(s) 700
TaiI ACGT 2 cut(s) 42, 854
TaqI TCGA 3 cut(s) 580, 597, 603
TatI WGTACW 2 cut(s) 181, 534
TauI GCSGC 5 cut(s) 246, 572, 610, 940, 954
TfiI GAWTC 2 cut(s) 383, 684
Tru1I TTAA 1 cut(s) 900
Tru9I TTAA 1 cut(s) 900
TscAI CASTG 1 cut(s) 771
TspDTI ATGAA 2 cut(s) 334, 720
TspRI CASTG 1 cut(s) 771
Van91I CCANNNNNTGG 1 cut(s) 732
VpaK11BI GGWCC 2 cut(s) 102, 828
XceI RCATGY 1 cut(s) 535
XcmI CCANNNNNNNNNTGG 1 cut(s) 91
XhoI CTCGAG 1 cut(s) 579
XmiI GTMKAC 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 382
ZrmI AGTACT 1 cut(s) 183
Zsp2I ATGCAT 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.