MD06G1019500.v1.1

Acts in the modification of cell walls via demethylesterification of cell wall pectin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0028299
Physical Location & Seq
Reverse (-)
2334216 .. 2334932
717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1019500.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGCTCAAGACATGACATTTGAAAACACGACCGGACCCGAGAAACATCAAGCCATCGCAATGCGCTATGGCACCAACCAATTGGTGTTTTACCGTTGTAGCTTCAACGGCTACCAAGATACATTCTACGCGAAACGCCAGTCACAGTTCTATCGTGACTCTGAGATATATTATTTTCGGCAACGCTGCAGTTGTGCTCCAAAACTGCAACATATACGTGGGAAGACCTATGAAAAATCAAGTGAATGTGGTGAAGGTTTGCAATCTAGGGAGCAGTCTGATCAGTCCACTGGCATTGTTATTCACAATTGCCATGTCACGGCCGCTGAAGATTTAAGACATGTGCAACACTCTTTCCGAACATATTTGGGCTGGTCGTTGTGGGTGTACCCAAGGACGGTGATCATGAAGAGTACTTTGAACGGATTGGTGGATCCTACCGGTCGGATGTTGTGTAATGGAAGTGTTGGCCTGTCCACACTTTACTATGGGGAGTACATGAACTTTGGAGGTTGTGCCGATACTTGCGGGAGGGTCAAGTTACTTGGTTATCACGTAATAAGGAGTACACCGGATGCCAAAAAATTTATTGTTAGGAACTTCATAAGTGGAGATTCATGGATTTCAAGAATAGGAGTACCCTTTCAACAGTGGTTTAGAGTTACCAAAATTAGTTTTGTAGTCCAGAAACTGTATACATATGCAATTGAATTGTAA

Protein Analysis

239

Amino Acids

27.49

Weight (kDa)

9.15

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 1 - 205 3.1e-55 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028299)

Species Orthologous Gene IDs
malus_domestica MD06G1019500.v1.1
pyrus_communis pycom06g01450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccI GTMKAC 1 cut(s) 695
AccII CGCG 1 cut(s) 131
AciI CCGC 2 cut(s) 324, 528
AclWI GGATC 2 cut(s) 428, 441
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 584
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 5 cut(s) 389, 415, 497, 568, 639
AfiI CCNNNNNNNGG 2 cut(s) 319, 397
AflIII ACRYGT 1 cut(s) 340
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 6 cut(s) 23, 106, 421, 627, 647, 710
AluBI AGCT 1 cut(s) 102
AluI AGCT 1 cut(s) 102
Alw21I GWGCWC 1 cut(s) 199
AlwI GGATC 2 cut(s) 428, 441
AlwNI CAGNNNCTG 1 cut(s) 691
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 2 cut(s) 321, 469
ApeKI GCWGC 1 cut(s) 186
ApoI RAATTY 1 cut(s) 584
ArsI GACNNNNNNTTYG 3 cut(s) 34, 125, 157
AsiGI ACCGGT 1 cut(s) 440
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 1 cut(s) 35
AsuHPI GGTGA 2 cut(s) 263, 412
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 1 cut(s) 35
BamHI GGATCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 199
BbvI GCAGC 1 cut(s) 173
BccI CCATC 1 cut(s) 62
BceAI ACGGC 2 cut(s) 124, 336
BclI TGATCA 2 cut(s) 280, 402
BfaI CTAG 1 cut(s) 267
BfmI CTRYAG 1 cut(s) 187
BisI GCNGC 2 cut(s) 187, 324
BlsI GCNGC 2 cut(s) 188, 325
BmcAI AGTACT 1 cut(s) 415
Bme18I GGWCC 1 cut(s) 35
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 35
BmiI GGNNCC 3 cut(s) 37, 73, 435
BmsI GCATC 1 cut(s) 565
BpiI GAAGAC 1 cut(s) 230
BsaAI YACGTR 2 cut(s) 218, 556
BsaJI CCNNGG 1 cut(s) 392
BsaWI WCCGGW 3 cut(s) 32, 440, 571
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 397
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 2 cut(s) 139, 295
Bse3DI GCAATG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 2 cut(s) 453, 580
BseLI CCNNNNNNNGG 2 cut(s) 319, 397
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 2 cut(s) 139, 295
BseX3I CGGCCG 1 cut(s) 321
BseXI GCAGC 1 cut(s) 173
Bsh1236I CGCG 1 cut(s) 131
Bsh1285I CGRYCG 3 cut(s) 33, 324, 445
BshFI GGCC 2 cut(s) 323, 471
BshNI GGYRCC 1 cut(s) 71
BshTI ACCGGT 1 cut(s) 440
BsiEI CGRYCG 3 cut(s) 33, 324, 445
BsiHKAI GWGCWC 1 cut(s) 199
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 3 cut(s) 33, 441, 572
BslI CCNNNNNNNGG 2 cut(s) 319, 397
BsnI GGCC 2 cut(s) 323, 471
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 199
Bsp143I GATC 3 cut(s) 280, 402, 433
BspACI CCGC 2 cut(s) 324, 528
BspANI GGCC 2 cut(s) 323, 471
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 1 cut(s) 131
BspHI TCATGA 1 cut(s) 405
BspLI GGNNCC 3 cut(s) 37, 73, 435
BspMAI CTGCAG 1 cut(s) 191
BspPI GGATC 2 cut(s) 428, 441
BspT107I GGYRCC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 66
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 2 cut(s) 139, 295
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 3 cut(s) 280, 402, 433
BssNAI GTATAC 1 cut(s) 696
BssT1I CCWWGG 1 cut(s) 392
Bst1107I GTATAC 1 cut(s) 696
Bst4CI ACNGT 5 cut(s) 95, 147, 400, 651, 693
Bst6I CTCTTC 1 cut(s) 404
BstBAI YACGTR 2 cut(s) 218, 556
BstDEI CTNAG 1 cut(s) 162
BstF5I GGATG 2 cut(s) 453, 580
BstFNI CGCG 1 cut(s) 131
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 3 cut(s) 283, 405, 436
BstMBI GATC 3 cut(s) 280, 402, 433
BstMCI CGRYCG 3 cut(s) 33, 324, 445
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 344
BstSFI CTRYAG 1 cut(s) 187
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 1 cut(s) 173
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 82
BstYI RGATCY 1 cut(s) 433
BstZ17I GTATAC 1 cut(s) 696
BstZI CGGCCG 1 cut(s) 321
BsuRI GGCC 2 cut(s) 323, 471
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 453, 580
BtsIMutI CAGTG 2 cut(s) 288, 656
CaiI CAGNNNCTG 1 cut(s) 691
CciI TCATGA 1 cut(s) 405
CfoI GCGC 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 1 cut(s) 35
Csp6I GTAC 5 cut(s) 388, 414, 496, 567, 638
CspAI ACCGGT 1 cut(s) 440
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviAII CATG 6 cut(s) 13, 314, 341, 406, 499, 618
CviJI RGCY 7 cut(s) 5, 53, 102, 111, 323, 372, 471
CviKI_1 RGCY 7 cut(s) 5, 53, 102, 111, 323, 372, 471
CviQI GTAC 5 cut(s) 388, 414, 496, 567, 638
DdeI CTNAG 1 cut(s) 162
DpnI GATC 3 cut(s) 282, 404, 435
DpnII GATC 3 cut(s) 280, 402, 433
EaeI YGGCCR 1 cut(s) 321
EagI CGGCCG 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
EclXI CGGCCG 1 cut(s) 321
Eco130I CCWWGG 1 cut(s) 392
Eco47I GGWCC 1 cut(s) 35
Eco52I CGGCCG 1 cut(s) 321
Eco57I CTGAAG 1 cut(s) 348
Eco88I CYCGRG 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 392
ErhI CCWWGG 1 cut(s) 392
FaeI CATG 6 cut(s) 16, 317, 344, 409, 502, 621
FatI CATG 6 cut(s) 12, 313, 340, 405, 498, 617
FauI CCCGC 1 cut(s) 521
FauNDI CATATG 1 cut(s) 700
FbaI TGATCA 2 cut(s) 280, 402
FblI GTMKAC 1 cut(s) 695
Fnu4HI GCNGC 2 cut(s) 187, 324
FokI GGATG 2 cut(s) 460, 587
Fsp4HI GCNGC 2 cut(s) 187, 324
FspBI CTAG 1 cut(s) 267
GlaI GCGC 1 cut(s) 65
GluI GCNGC 2 cut(s) 187, 324
HaeIII GGCC 2 cut(s) 323, 471
HapII CCGG 3 cut(s) 33, 441, 572
HhaI GCGC 1 cut(s) 66
Hin1II CATG 6 cut(s) 16, 317, 344, 409, 502, 621
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HinfI GANTC 2 cut(s) 158, 614
HpaII CCGG 3 cut(s) 33, 441, 572
HphI GGTGA 2 cut(s) 263, 412
Hpy166II GTNNAC 5 cut(s) 288, 388, 477, 569, 696
Hpy188I TCNGA 4 cut(s) 163, 280, 359, 447
Hpy188III TCNNGA 5 cut(s) 8, 155, 406, 627, 685
Hpy8I GTNNAC 5 cut(s) 288, 388, 477, 569, 696
HpyAV CCTTC 1 cut(s) 248
HpyCH4III ACNGT 5 cut(s) 95, 147, 400, 651, 693
HpyCH4IV ACGT 2 cut(s) 217, 555
HpyCH4V TGCA 5 cut(s) 189, 208, 262, 346, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 2 cut(s) 217, 555
Hsp92II CATG 6 cut(s) 16, 317, 344, 409, 502, 621
HspAI GCGC 1 cut(s) 64
Ksp22I TGATCA 2 cut(s) 280, 402
Kzo9I GATC 3 cut(s) 280, 402, 433
LmnI GCTCC 2 cut(s) 202, 271
LpnPI CCDG 8 cut(s) 46, 152, 276, 358, 454, 485, 585, 698
Lsp1109I GCAGC 1 cut(s) 173
LweI GCATC 1 cut(s) 565
MaeI CTAG 1 cut(s) 267
MaeII ACGT 2 cut(s) 217, 555
MaeIII GTNAC 5 cut(s) 141, 155, 316, 540, 661
MalI GATC 3 cut(s) 282, 404, 435
MboI GATC 3 cut(s) 280, 402, 433
MboII GAAGA 3 cut(s) 235, 341, 421
MfeI CAATTG 3 cut(s) 80, 307, 705
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 1 cut(s) 199
MluCI AATT 6 cut(s) 80, 307, 584, 669, 705, 710
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 425
MnlI CCTC 2 cut(s) 503, 525
MseI TTAA 1 cut(s) 335
MslI CAYNNNNRTG 2 cut(s) 59, 216
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 3 cut(s) 33, 441, 572
MunI CAATTG 3 cut(s) 80, 307, 705
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 1 cut(s) 108
NdeI CATATG 1 cut(s) 700
NdeII GATC 3 cut(s) 280, 402, 433
NlaIII CATG 6 cut(s) 16, 317, 344, 409, 502, 621
NlaIV GGNNCC 3 cut(s) 37, 73, 435
NmuCI GTSAC 3 cut(s) 141, 155, 316
NspI RCATGY 1 cut(s) 344
PagI TCATGA 1 cut(s) 405
PciI ACATGT 1 cut(s) 340
PfeI GAWTC 1 cut(s) 614
PinAI ACCGGT 1 cut(s) 440
PkrI GCNGC 2 cut(s) 188, 325
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Ppu21I YACGTR 2 cut(s) 218, 556
PscI ACATGT 1 cut(s) 340
PspN4I GGNNCC 3 cut(s) 37, 73, 435
PspPI GGNCC 1 cut(s) 35
PstI CTGCAG 1 cut(s) 191
PstNI CAGNNNCTG 1 cut(s) 691
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 5 cut(s) 389, 415, 497, 568, 639
RsaNI GTAC 5 cut(s) 388, 414, 496, 567, 638
RseI CAYNNNNRTG 2 cut(s) 59, 216
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 2 cut(s) 187, 324
Sau3AI GATC 3 cut(s) 280, 402, 433
Sau96I GGNCC 1 cut(s) 35
ScaI AGTACT 1 cut(s) 415
SchI GAGTC 1 cut(s) 152
SduI GDGCHC 1 cut(s) 199
SetI ASST 6 cut(s) 104, 220, 230, 259, 514, 558
SfaNI GCATC 1 cut(s) 565
SfcI CTRYAG 1 cut(s) 187
SinI GGWCC 1 cut(s) 35
SmiMI CAYNNNNRTG 2 cut(s) 59, 216
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
Sse9I AATT 6 cut(s) 80, 307, 584, 669, 705, 710
SsiI CCGC 2 cut(s) 324, 528
SspMI CTAG 1 cut(s) 267
StyI CCWWGG 1 cut(s) 392
TaaI ACNGT 5 cut(s) 95, 147, 400, 651, 693
TaiI ACGT 2 cut(s) 220, 558
TasI AATT 6 cut(s) 80, 307, 584, 669, 705, 710
TatI WGTACW 3 cut(s) 413, 495, 566
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 2 cut(s) 295, 656
TseFI GTSAC 3 cut(s) 141, 155, 316
TseI GCWGC 1 cut(s) 186
Tsp45I GTSAC 3 cut(s) 141, 155, 316
TspDTI ATGAA 5 cut(s) 246, 422, 515, 592, 606
TspGWI ACGGA 1 cut(s) 438
TspRI CASTG 2 cut(s) 295, 656
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 1 cut(s) 584
XceI RCATGY 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 695
XspI CTAG 1 cut(s) 267
ZrmI AGTACT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.