Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0022313

Physical Location & Seq

Genomic Coordinates Forward (+)

20803943 .. 20807520

Gene Structure 3578 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1084800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGCTTCACTCCGTTAAGATCTCCCAAGGCCGAGCAACACTTTGCAGCCGCTACGTCAAGACCTATAAATATACCACAGAGCGCAAAACTGGTTATCCTTTCTTTCCCAGCATCTTCTCTAGCTTCAACGGCCTTCTTGCCTCTACCATGCGCGCAGGTCTCTTCGCTTCCCAAATCATTACTGGTCAGCTCAATCCTGCAAACGGTTTTGGTGTCGCAAACACCAATTTAGCTTTGTTTGGCAATCGTATCTATGCGCTTTGCGAGGTTGACCTCCCATACGCTGTGAGTTTGACATCGAACGGAGATATTCAAACCCTGGGGCGCTGTGATTTTAATGAGAAACTCTCCGTGAGCATGACAGCTCATCCGAAGATAGACCCAGAAACCGGCGAGACCTTTGCTTTTCGCTTCAGCGCGGTGCGTCCATTCCTCACCTATTTTCACTTTGATGCAGATGGGACAAAGCACCCGGACGTGCCCATAATCTCTATGCGTCGACCATCTATCATCCACGACTTTGCAATCACCAAGAAATATGCCGTATTTGTTGACATACAAATAACATTAGACCCCATCAAAGTGATCACTACCGGAGGATCGCCAGTCGTTTTTGATACCTCCAAAGTTCCTAGGATTGGAGTACTTCCCCGGTACGCAGTTGACGAGTCCGAGATAAGATGGTTTGATGTTCCGGGGTTCAACATTTTACACACCATTAATGCATGGGAAGACGAAGAGGATACGGATACCATCGTGTTGAAGGTGAGGATCAATCTTAAGACGGGGATAGTAATGAGGCTGCCCATCTCCATAAGGAATGTAGAACTCGGGGTGATTAACCAGGCTTACGTGGGGAAGAAGAATAAGTTTGTGTATGGATCTGTTGCTGAGGAATCAATGCTCCCTGATCACGATTCAATACCCAAGTGTGCAGAGTTGGTGAAGCTGGATCTCGATGTGTCGTCCAATAGGGATGGGCGTACGGTGGCAAGCAGGATATACGGCCCAAATTGCTATGGGGGCGAGCCTTTCTTTGTTGCTAGAGAACCGGAGAACCCTAACGCAGAGGAGGATGACGGATACTTGGTGTCATTTGTGCATGATGAGAAGACAGGAGAATCAAGATTCTTGGTGATGGATGCTAAGTCCTCACAGCTTGATATAGTGGCGGTTCTCAAGCTGCCCCGCCGGGTTCCTTACGGCTTCCATGGTCTCTTCGTGAAGGAAAAGGACCTAGAAAATATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

RPE65

Protein Analysis

417

Amino Acids

46.48

Weight (kDa)

6.17

Isoelectric Point (pI)

28.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPE65	PF03055	1 - 254	3.8e-60	Retinal pigment epithelial membrane protein
RPE65	PF03055	255 - 408	2.3e-34	Retinal pigment epithelial membrane protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0022313)

Species	Orthologous Gene IDs
malus_domestica	MD05G1057000.v1.1 MD06G1084800.v1.1 MD14G1105700.v1.1
pyrus_communis	pycom14g10120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	146
AccI	GTMKAC	1 cut(s)	499
AccII	CGCG	2 cut(s)	153, 419
AciI	CCGC	4 cut(s)	49, 419, 1172, 1189
AclWI	GGATC	4 cut(s)	607, 779, 889, 960
AcuI	CTGAAG	1 cut(s)	397
AfaI	GTAC	3 cut(s)	645, 656, 985
AfiI	CCNNNNNNNGG	3 cut(s)	203, 389, 638
AflII	CTTAAG	1 cut(s)	779
AgsI	TTSAA	5 cut(s)	127, 314, 703, 763, 921
AjiI	CACGTC	1 cut(s)	478
AjnI	CCWGG	2 cut(s)	318, 843
AluBI	AGCT	7 cut(s)	123, 190, 233, 365, 949, 1159, 1183
AluI	AGCT	7 cut(s)	123, 190, 233, 365, 949, 1159, 1183
Alw26I	GTCTC	3 cut(s)	164, 389, 1220
AlwI	GGATC	4 cut(s)	607, 779, 889, 960
Ama87I	CYCGRG	1 cut(s)	830
AoxI	GGCC	3 cut(s)	28, 130, 1006
ApeKI	GCWGC	3 cut(s)	45, 802, 1183
AseI	ATTAAT	1 cut(s)	720
AspA2I	CCTAGG	1 cut(s)	632
AspLEI	GCGC	6 cut(s)	84, 153, 155, 259, 327, 419
AspS9I	GGNCC	2 cut(s)	1007, 1234
AsuC2I	CCSGG	4 cut(s)	473, 652, 696, 1193
AsuHPI	GGTGA	6 cut(s)	427, 520, 778, 847, 955, 1147
AvaI	CYCGRG	1 cut(s)	830
AvaII	GGWCC	1 cut(s)	1234
AvrII	CCTAGG	1 cut(s)	632
BaeGI	GKGCMC	1 cut(s)	483
BaeI	ACNNNNGTAYC	2 cut(s)	741, 774
BbsI	GAAGAC	2 cut(s)	738, 1118
BbvCI	CCTCAGC	1 cut(s)	891
BbvI	GCAGC	3 cut(s)	57, 789, 1170
BccI	CCATC	8 cut(s)	452, 511, 584, 675, 761, 815, 971, 1132
BceAI	ACGGC	4 cut(s)	145, 527, 1021, 1219
BcgI	CGANNNNNNTGC	2 cut(s)	383, 417
BciT130I	CCWGG	2 cut(s)	320, 845
BciVI	GTATCC	3 cut(s)	736, 742, 1076
BclI	TGATCA	2 cut(s)	585, 910
BcnI	CCSGG	4 cut(s)	473, 652, 696, 1193
BcoDI	GTCTC	3 cut(s)	164, 389, 1220
BfaI	CTAG	4 cut(s)	120, 633, 1044, 1238
BfoI	RGCGCY	1 cut(s)	328
BfrI	CTTAAG	1 cut(s)	779
BfuAI	ACCTGC	1 cut(s)	146
BfuI	GTATCC	3 cut(s)	736, 742, 1076
BglII	AGATCT	1 cut(s)	18
BisI	GCNGC	4 cut(s)	46, 49, 803, 1184
BlnI	CCTAGG	1 cut(s)	632
BlsI	GCNGC	4 cut(s)	47, 50, 804, 1185
BmcAI	AGTACT	1 cut(s)	645
Bme1390I	CCNGG	6 cut(s)	320, 473, 652, 696, 845, 1193
Bme18I	GGWCC	1 cut(s)	1234
BmeT110I	CYCGRG	1 cut(s)	830
BmgBI	CACGTC	1 cut(s)	478
BmgT120I	GGNCC	2 cut(s)	1007, 1234
BmiI	GGNNCC	1 cut(s)	1197
BmrFI	CCNGG	6 cut(s)	320, 473, 652, 696, 845, 1193
BmsI	GCATC	3 cut(s)	120, 442, 1132
BpiI	GAAGAC	2 cut(s)	738, 1118
BplI	GAGNNNNNCTC	2 cut(s)	332, 364
Bpu10I	CCTNAGC	1 cut(s)	891
BpuEI	CTTGAG	1 cut(s)	1163
BpuMI	CCSGG	4 cut(s)	473, 652, 696, 1193
BsaAI	YACGTR	1 cut(s)	853
BsaI	GGTCTC	3 cut(s)	164, 389, 1220
BsaJI	CCNNGG	7 cut(s)	25, 318, 319, 632, 650, 695, 1210
BsaWI	WCCGGW	2 cut(s)	593, 1051
Bsc4I	CCNNNNNNNGG	3 cut(s)	203, 389, 638
Bse118I	RCCGGY	1 cut(s)	389
Bse1I	ACTGG	3 cut(s)	94, 187, 605
BseBI	CCWGG	2 cut(s)	320, 845
BseDI	CCNNGG	7 cut(s)	25, 318, 319, 632, 650, 695, 1210
BseGI	GGATG	5 cut(s)	367, 510, 982, 1081, 1147
BseLI	CCNNNNNNNGG	3 cut(s)	203, 389, 638
BseMII	CTCAG	1 cut(s)	882
BseNI	ACTGG	3 cut(s)	94, 187, 605
BsePI	GCGCGC	1 cut(s)	151
BseRI	GAGGAG	1 cut(s)	1085
BseSI	GKGCMC	1 cut(s)	483
BseXI	GCAGC	3 cut(s)	57, 789, 1170
BseYI	CCCAGC	1 cut(s)	107
BsgI	GTGCAG	1 cut(s)	954
Bsh1236I	CGCG	2 cut(s)	153, 419
BshFI	GGCC	3 cut(s)	30, 132, 1008
BsiHKCI	CYCGRG	1 cut(s)	830
BsiSI	CCGG	7 cut(s)	390, 473, 594, 652, 695, 1052, 1192
BsiWI	CGTACG	1 cut(s)	983
BslFI	GGGAC	1 cut(s)	475
BslI	CCNNNNNNNGG	3 cut(s)	203, 389, 638
BsmAI	GTCTC	3 cut(s)	164, 389, 1220
BsmFI	GGGAC	1 cut(s)	475
BsnI	GGCC	3 cut(s)	30, 132, 1008
Bso31I	GGTCTC	3 cut(s)	164, 389, 1220
BsoBI	CYCGRG	1 cut(s)	830
Bsp1286I	GDGCHC	1 cut(s)	483
Bsp143I	GATC	7 cut(s)	18, 585, 599, 771, 881, 910, 952
Bsp19I	CCATGG	1 cut(s)	1210
BspACI	CCGC	4 cut(s)	49, 419, 1172, 1189
BspANI	GGCC	3 cut(s)	30, 132, 1008
BspCNI	CTCAG	1 cut(s)	883
BspFNI	CGCG	2 cut(s)	153, 419
BspLI	GGNNCC	1 cut(s)	1197
BspMI	ACCTGC	1 cut(s)	146
BspPI	GGATC	4 cut(s)	607, 779, 889, 960
BspTI	CTTAAG	1 cut(s)	779
BspTNI	GGTCTC	3 cut(s)	164, 389, 1220
BsrFI	RCCGGY	1 cut(s)	389
BsrI	ACTGG	3 cut(s)	94, 187, 605
BssAI	RCCGGY	1 cut(s)	389
BssECI	CCNNGG	7 cut(s)	25, 318, 319, 632, 650, 695, 1210
BssHII	GCGCGC	1 cut(s)	151
BssMI	GATC	7 cut(s)	18, 585, 599, 771, 881, 910, 952
BssT1I	CCWWGG	3 cut(s)	25, 632, 1210
Bst2UI	CCWGG	2 cut(s)	320, 845
Bst4CI	ACNGT	2 cut(s)	206, 988
Bst6I	CTCTTC	3 cut(s)	167, 732, 1223
BstAFI	CTTAAG	1 cut(s)	779
BstBAI	YACGTR	1 cut(s)	853
BstC8I	GCNNGC	3 cut(s)	153, 994, 1028
BstDEI	CTNAG	2 cut(s)	891, 1146
BstDSI	CCRYGG	1 cut(s)	1210
BstF5I	GGATG	5 cut(s)	367, 510, 982, 1081, 1147
BstFNI	CGCG	2 cut(s)	153, 419
BstH2I	RGCGCY	1 cut(s)	328
BstHHI	GCGC	6 cut(s)	84, 153, 155, 259, 327, 419
BstKTI	GATC	7 cut(s)	21, 588, 602, 774, 884, 913, 955
BstMAI	GTCTC	3 cut(s)	164, 389, 1220
BstMBI	GATC	7 cut(s)	18, 585, 599, 771, 881, 910, 952
BstMWI	GCNNNNNNNGC	3 cut(s)	129, 1014, 1023
BstNI	CCWGG	2 cut(s)	320, 845
BstSCI	CCNGG	6 cut(s)	318, 471, 650, 694, 843, 1191
BstSLI	GKGCMC	1 cut(s)	483
BstUI	CGCG	2 cut(s)	153, 419
BstV1I	GCAGC	3 cut(s)	57, 789, 1170
BstV2I	GAAGAC	2 cut(s)	738, 1118
BstX2I	RGATCY	3 cut(s)	18, 881, 952
BstYI	RGATCY	3 cut(s)	18, 881, 952
BsuI	GTATCC	3 cut(s)	736, 742, 1076
BsuRI	GGCC	3 cut(s)	30, 132, 1008
BtgI	CCRYGG	1 cut(s)	1210
BtrI	CACGTC	1 cut(s)	478
BtsCI	GGATG	5 cut(s)	367, 510, 982, 1081, 1147
BveI	ACCTGC	1 cut(s)	146
Cac8I	GCNNGC	3 cut(s)	153, 994, 1028
CfoI	GCGC	6 cut(s)	84, 153, 155, 259, 327, 419
Cfr10I	RCCGGY	1 cut(s)	389
Cfr13I	GGNCC	2 cut(s)	1007, 1234
CseI	GACGC	2 cut(s)	413, 485
Csp6I	GTAC	3 cut(s)	644, 655, 984
CviAII	CATG	5 cut(s)	148, 358, 726, 1103, 1211
CviQI	GTAC	3 cut(s)	644, 655, 984
DdeI	CTNAG	2 cut(s)	891, 1146
DpnI	GATC	7 cut(s)	20, 587, 601, 773, 883, 912, 954
DpnII	GATC	7 cut(s)	18, 585, 599, 771, 881, 910, 952
Eam1104I	CTCTTC	3 cut(s)	167, 732, 1223
EarI	CTCTTC	3 cut(s)	167, 732, 1223
Eco130I	CCWWGG	3 cut(s)	25, 632, 1210
Eco31I	GGTCTC	3 cut(s)	164, 389, 1220
Eco47I	GGWCC	1 cut(s)	1234
Eco57I	CTGAAG	1 cut(s)	397
Eco88I	CYCGRG	1 cut(s)	830
EcoO109I	RGGNCCY	1 cut(s)	1234
EcoRII	CCWGG	2 cut(s)	318, 843
EcoT14I	CCWWGG	3 cut(s)	25, 632, 1210
EcoT22I	ATGCAT	1 cut(s)	727
ErhI	CCWWGG	3 cut(s)	25, 632, 1210
FaeI	CATG	5 cut(s)	151, 361, 729, 1106, 1214
FaqI	GGGAC	1 cut(s)	475
FatI	CATG	5 cut(s)	147, 357, 725, 1102, 1210
FauI	CCCGC	1 cut(s)	1196
FbaI	TGATCA	2 cut(s)	585, 910
FblI	GTMKAC	1 cut(s)	499
Fnu4HI	GCNGC	4 cut(s)	46, 49, 803, 1184
FokI	GGATG	5 cut(s)	354, 497, 989, 1088, 1154
Fsp4HI	GCNGC	4 cut(s)	46, 49, 803, 1184
FspBI	CTAG	4 cut(s)	120, 633, 1044, 1238
GlaI	GCGC	6 cut(s)	83, 152, 154, 258, 326, 418
GluI	GCNGC	4 cut(s)	46, 49, 803, 1184
GsaI	CCCAGC	1 cut(s)	111
HaeII	RGCGCY	1 cut(s)	328
HaeIII	GGCC	3 cut(s)	30, 132, 1008
HapII	CCGG	7 cut(s)	390, 473, 594, 652, 695, 1052, 1192
HgaI	GACGC	2 cut(s)	413, 485
HhaI	GCGC	6 cut(s)	84, 153, 155, 259, 327, 419
Hin1II	CATG	5 cut(s)	151, 361, 729, 1106, 1214
Hin6I	GCGC	6 cut(s)	82, 151, 153, 257, 325, 417
HinP1I	GCGC	6 cut(s)	82, 151, 153, 257, 325, 417
HincII	GTYRAC	4 cut(s)	271, 500, 553, 664
HindII	GTYRAC	4 cut(s)	271, 500, 553, 664
HinfI	GANTC	5 cut(s)	668, 896, 917, 1121, 1128
HpaII	CCGG	7 cut(s)	390, 473, 594, 652, 695, 1052, 1192
HphI	GGTGA	6 cut(s)	427, 520, 778, 847, 955, 1147
Hpy166II	GTNNAC	4 cut(s)	271, 500, 553, 664
Hpy188I	TCNGA	2 cut(s)	372, 673
Hpy188III	TCNNGA	5 cut(s)	58, 914, 956, 1125, 1222
Hpy8I	GTNNAC	4 cut(s)	271, 500, 553, 664
Hpy99I	CGWCG	1 cut(s)	501
HpyAV	CCTTC	3 cut(s)	143, 757, 1219
HpyCH4III	ACNGT	2 cut(s)	206, 988
HpyCH4IV	ACGT	3 cut(s)	54, 477, 852
HpyCH4V	TGCA	7 cut(s)	45, 200, 455, 524, 725, 935, 1102
HpyF10VI	GCNNNNNNNGC	3 cut(s)	129, 1014, 1023
HpyF3I	CTNAG	2 cut(s)	891, 1146
HpySE526I	ACGT	3 cut(s)	54, 477, 852
Hsp92II	CATG	5 cut(s)	151, 361, 729, 1106, 1214
HspAI	GCGC	6 cut(s)	82, 151, 153, 257, 325, 417
Ksp22I	TGATCA	2 cut(s)	585, 910
Kzo9I	GATC	7 cut(s)	18, 585, 599, 771, 881, 910, 952
LmnI	GCTCC	1 cut(s)	909
Lsp1109I	GCAGC	3 cut(s)	57, 789, 1170
LweI	GCATC	3 cut(s)	120, 442, 1132
MaeI	CTAG	4 cut(s)	120, 633, 1044, 1238
MaeII	ACGT	3 cut(s)	54, 477, 852
MalI	GATC	7 cut(s)	20, 587, 601, 773, 883, 912, 954
MboI	GATC	7 cut(s)	18, 585, 599, 771, 881, 910, 952
MboII	GAAGA	9 cut(s)	106, 154, 385, 743, 749, 871, 874, 1123, 1210
MflI	RGATCY	3 cut(s)	18, 881, 952
MhlI	GDGCHC	1 cut(s)	483
MluCI	AATT	2 cut(s)	226, 1012
MlyI	GAGTC	1 cut(s)	677
Mph1103I	ATGCAT	1 cut(s)	727
MseI	TTAA	5 cut(s)	15, 336, 720, 780, 840
MslI	CAYNNNNRTG	2 cut(s)	450, 581
MspCI	CTTAAG	1 cut(s)	779
MspI	CCGG	7 cut(s)	390, 473, 594, 652, 695, 1052, 1192
MspR9I	CCNGG	6 cut(s)	320, 473, 652, 696, 845, 1193
MvaI	CCWGG	2 cut(s)	320, 845
MvnI	CGCG	2 cut(s)	153, 419
MwoI	GCNNNNNNNGC	3 cut(s)	129, 1014, 1023
NciI	CCSGG	4 cut(s)	473, 652, 696, 1193
NcoI	CCATGG	1 cut(s)	1210
NdeII	GATC	7 cut(s)	18, 585, 599, 771, 881, 910, 952
NlaIII	CATG	5 cut(s)	151, 361, 729, 1106, 1214
NlaIV	GGNNCC	1 cut(s)	1197
NmeAIII	GCCGAG	1 cut(s)	56
NsiI	ATGCAT	1 cut(s)	727
PasI	CCCWGGG	1 cut(s)	319
PauI	GCGCGC	1 cut(s)	151
PcsI	WCGNNNNNNNCGW	1 cut(s)	663
PfeI	GAWTC	4 cut(s)	896, 917, 1121, 1128
Pfl23II	CGTACG	1 cut(s)	983
PkrI	GCNGC	4 cut(s)	47, 50, 804, 1185
PleI	GAGTC	1 cut(s)	676
PpsI	GAGTC	1 cut(s)	676
Ppu21I	YACGTR	1 cut(s)	853
PpuMI	RGGWCCY	1 cut(s)	1234
PshBI	ATTAAT	1 cut(s)	720
Psp5II	RGGWCCY	1 cut(s)	1234
Psp6I	CCWGG	2 cut(s)	318, 843
PspFI	CCCAGC	1 cut(s)	107
PspGI	CCWGG	2 cut(s)	318, 843
PspLI	CGTACG	1 cut(s)	983
PspN4I	GGNNCC	1 cut(s)	1197
PspPI	GGNCC	2 cut(s)	1007, 1234
PspPPI	RGGWCCY	1 cut(s)	1234
PsuI	RGATCY	3 cut(s)	18, 881, 952
PteI	GCGCGC	1 cut(s)	151
RsaI	GTAC	3 cut(s)	645, 656, 985
RsaNI	GTAC	3 cut(s)	644, 655, 984
RseI	CAYNNNNRTG	2 cut(s)	450, 581
SalI	GTCGAC	1 cut(s)	498
SaqAI	TTAA	5 cut(s)	15, 336, 720, 780, 840
SatI	GCNGC	4 cut(s)	46, 49, 803, 1184
Sau3AI	GATC	7 cut(s)	18, 585, 599, 771, 881, 910, 952
Sau96I	GGNCC	2 cut(s)	1007, 1234
ScaI	AGTACT	1 cut(s)	645
SchI	GAGTC	1 cut(s)	677
ScrFI	CCNGG	6 cut(s)	320, 473, 652, 696, 845, 1193
SduI	GDGCHC	1 cut(s)	483
SfaNI	GCATC	3 cut(s)	120, 442, 1132
SinI	GGWCC	1 cut(s)	1234
SmiMI	CAYNNNNRTG	2 cut(s)	450, 581
SmlI	CTYRAG	2 cut(s)	779, 1178
SmoI	CTYRAG	2 cut(s)	779, 1178
Sse9I	AATT	2 cut(s)	226, 1012
SsiI	CCGC	4 cut(s)	49, 419, 1172, 1189
SspMI	CTAG	4 cut(s)	120, 633, 1044, 1238
StyD4I	CCNGG	6 cut(s)	318, 471, 650, 694, 843, 1191
StyI	CCWWGG	3 cut(s)	25, 632, 1210
TaaI	ACNGT	2 cut(s)	206, 988
TaiI	ACGT	3 cut(s)	57, 480, 855
TaqI	TCGA	3 cut(s)	299, 499, 957
TasI	AATT	2 cut(s)	226, 1012
TatI	WGTACW	1 cut(s)	643
TauI	GCSGC	1 cut(s)	51
TfiI	GAWTC	4 cut(s)	896, 917, 1121, 1128
Tru1I	TTAA	5 cut(s)	15, 336, 720, 780, 840
Tru9I	TTAA	5 cut(s)	15, 336, 720, 780, 840
TseI	GCWGC	3 cut(s)	45, 802, 1183
TspGWI	ACGGA	4 cut(s)	318, 340, 761, 1095
Vha464I	CTTAAG	1 cut(s)	779
VpaK11BI	GGWCC	1 cut(s)	1234
VspI	ATTAAT	1 cut(s)	720
XcmI	CCANNNNNNNNNTGG	1 cut(s)	179
XmaJI	CCTAGG	1 cut(s)	632
XmiI	GTMKAC	1 cut(s)	499
XspI	CTAG	4 cut(s)	120, 633, 1044, 1238
ZrmI	AGTACT	1 cut(s)	645
Zsp2I	ATGCAT	1 cut(s)	727

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.