MD14G1105700.v1.1

carotenoid cleavage dioxygenase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0022313
Physical Location & Seq
Forward (+)
16432624 .. 16434390
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1105700.v1.1.491

Sequence Viewer

Length: 1767 bp
ATGATCCCAATCTCCATGGATGCGTTGTTTTCTTCTTTTTTGCCCACAATTTCCACTTATAAAAATCTCAATAATCCGAACACAAGAAAACTACAACCTCCAAAGAGAACTAGTCCTTCTCCATCCTTACCCGTACTAGCTGCTATAGCAACAAATGTTGATGATGATATTGCTCCAAAGAAACGATGTGCGATGATCTTGAAATCTCTGGATGACTTCATAAACAACTCCATAGAGCCTCCAATTCTTCGACCTTCAATCGACCGGAGGCTAGTAGTCTCCGACAATTTTGCTCCGGTCACCCAAGAGCTTCCCCCAACCGAGTGCGAGCTGATACATGGCTCTCTGCCACCATGCCTTGACGGTGCCTACATCCGCAACGGCCCTAACCCCCAATTCCTTCCCCAAGGGCGGCCTTACCACCTTTTCGATGGCGACGGCATGCTTCACTCCATCTTGATCTCCCAAGGCCGAGCAACACTTTGCAGCCGCTACGTCGAGACCTATAAATACACCGTGGAGCGCGAAGCCGGATATCCTCTCTTTCCCAGCATCTACTCTTGCTTCAACGGCCTCCTTGCTTCTACCATGCGCGCGGCTCTCCTGGCTACTCGAATCATTATTGGTCAGCTGAATCCTGCTAACGGCTTTGGTGTCGCAAACACCAGTTTAGCTCTCTTTGGCAACCGTCTCTATGCGCTTTGCGAGTCTGACCTTCCTTACGCCATGCGATTGACATCGAACGGAGATATACAAACTCTGGGGCGGCATGATTTCGACCAGAAGCTCTCCACGAGTATGACAGCTCATCCAAAGACAGACCCCGAAACAGGAGAAACCTTTGCTTTCCGCTACAGCCCCGTGTTTCCATACCTCACCTATTTTCACTTCGATGCAGACGGAACAAAGCACCCGGACGTGCCCATATTCTCTATGCGCCATCGATCTATCCTCCACGACTTTGCAATCACCAAGAAATATACCATATTTGTTGACATACAAATGACAGTGAATCCCATTAAAATGATCACTACGGGAGGAACTCCAGTCGTATTTGATCCCTCCAAAGTGCCTAAGATCGGAGTACTTCCCAGGTACGCAGTTGACGAGTCTGAGATAAAGTGGTTTGATGTTCCGGGGTTCAACATCTTGCACACCATTAATGCATGGGACGATGATCAAGAAGATGATAGCATTGTTCTGGTTGCACCAAATGTACTGTCCGTACAATACGTTTTTGAGAGATGGGATCTGTTCCGTGCATCGGTAGAGAAAGTGAGGATCAATCTTAAGACAGGGATCGTGACAAGGGAACCCATCTCCATAAGGAATCTAGAATTCGGAGTGATCAATCGGGCTTACGTGGGGAAAAAGAACAAGTTTGTGTATGGATCTATCTCTGAAGAATCAATGCACCCTGATGATTCAATACCGAAGTGTGCAGGGTTGGTGAAGCTGGATCTAGATGTGTCGTCCAACGGGGATGAACCCACGTTGGCCTGCAGGATGTACGGACCAAATTGCTATGGCGGTGAGCCTTTCTTTGTTGCTAGAGAGCCGGAGAATCCAAACGTGGAGGAGGATGATGGGTACGTGGTGTCATTTGTGCATGATGAGAAGACAGGAGAATCTAAGCTCTTAGTGATGGATGCGAAGTCCCCGCAGCTTGATATAGTGGCGGCTCTGAAGCTGCCCCGCCGGGTTCCTTACGGCTTCCATGGCCTCTTCGTGAAGGAAAGCGACCTTCAAAAGTTGAATGAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
RPE65

Protein Analysis

589

Amino Acids

65.7

Weight (kDa)

5.69

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 97 - 577 4.9e-124 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022313)

Species Orthologous Gene IDs
malus_domestica MD05G1057000.v1.1 MD06G1084800.v1.1 MD14G1105700.v1.1
pyrus_communis pycom14g10120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 60
AccB1I GGYRCC 1 cut(s) 365
AccII CGCG 3 cut(s) 525, 594, 596
AclWI GGATC 6 cut(s) 1052, 1257, 1289, 1307, 1399, 1467
AcsI RAATTY 1 cut(s) 1337
AcuI CTGAAG 2 cut(s) 1422, 1706
AfaI GTAC 7 cut(s) 135, 1086, 1097, 1218, 1227, 1511, 1592
AfiI CCNNNNNNNGG 5 cut(s) 411, 644, 830, 1079, 1264
AflII CTTAAG 1 cut(s) 1289
AgsI TTSAA 7 cut(s) 202, 258, 568, 1144, 1428, 1748, 1756
AhlI ACTAGT 1 cut(s) 110
AjiI CACGTC 1 cut(s) 919
AjnI CCWGG 2 cut(s) 603, 1091
Alw26I GTCTC 3 cut(s) 283, 494, 695
AlwI GGATC 6 cut(s) 1052, 1257, 1289, 1307, 1399, 1467
AoxI GGCC 6 cut(s) 382, 413, 469, 571, 1497, 1720
ApeKI GCWGC 4 cut(s) 140, 486, 1663, 1690
ApoI RAATTY 1 cut(s) 1337
ArsI GACNNNNNNTTYG 2 cut(s) 1205, 1237
AseI ATTAAT 1 cut(s) 1161
AspLEI GCGC 5 cut(s) 525, 594, 596, 700, 939
AspS9I GGNCC 2 cut(s) 383, 1514
AsuC2I CCSGG 3 cut(s) 914, 1137, 1700
AsuHPI GGTGA 5 cut(s) 292, 868, 961, 1462, 1544
AvaII GGWCC 1 cut(s) 1514
BaeGI GKGCMC 1 cut(s) 924
BanI GGYRCC 1 cut(s) 365
BauI CACGAG 1 cut(s) 793
BbsI GAAGAC 1 cut(s) 1625
BbvI GCAGC 4 cut(s) 127, 498, 1675, 1677
BccI CCATC 8 cut(s) 130, 425, 461, 948, 1239, 1325, 1580, 1639
BceAI ACGGC 5 cut(s) 397, 454, 586, 661, 1726
BcgI CGANNNNNNTGC 2 cut(s) 272, 306
BciT130I CCWGG 2 cut(s) 605, 1093
BclI TGATCA 3 cut(s) 1026, 1177, 1347
BcnI CCSGG 3 cut(s) 914, 1137, 1700
BcoDI GTCTC 3 cut(s) 283, 494, 695
BcuI ACTAGT 1 cut(s) 110
BfaI CTAG 6 cut(s) 111, 137, 272, 1334, 1463, 1551
BfmI CTRYAG 3 cut(s) 144, 853, 1501
BfrI CTTAAG 1 cut(s) 1289
BisI GCNGC 9 cut(s) 141, 413, 487, 490, 597, 767, 1664, 1680, 1691
BlsI GCNGC 9 cut(s) 142, 414, 488, 491, 598, 768, 1665, 1681, 1692
BmcAI AGTACT 1 cut(s) 1086
Bme1390I CCNGG 5 cut(s) 605, 914, 1093, 1137, 1700
Bme18I GGWCC 1 cut(s) 1514
BmgBI CACGTC 1 cut(s) 919
BmgT120I GGNCC 2 cut(s) 383, 1514
BmiI GGNNCC 3 cut(s) 367, 1314, 1704
BmrFI CCNGG 5 cut(s) 605, 914, 1093, 1137, 1700
BmsI GCATC 5 cut(s) 10, 561, 883, 1271, 1639
BpiI GAAGAC 1 cut(s) 1625
BpmI CTGGAG 1 cut(s) 1029
BpuMI CCSGG 3 cut(s) 914, 1137, 1700
Bsa29I ATCGAT 1 cut(s) 943
BsaAI YACGTR 2 cut(s) 1363, 1594
BsaBI GATNNNNATC 2 cut(s) 8, 736
BsaI GGTCTC 1 cut(s) 494
BsaJI CCNNGG 7 cut(s) 15, 406, 466, 516, 1091, 1136, 1717
BsaWI WCCGGW 2 cut(s) 264, 295
Bsc4I CCNNNNNNNGG 5 cut(s) 411, 644, 830, 1079, 1264
Bse1I ACTGG 2 cut(s) 666, 1046
Bse8I GATNNNNATC 2 cut(s) 8, 736
BseBI CCWGG 2 cut(s) 605, 1093
BseCI ATCGAT 1 cut(s) 943
BseDI CCNNGG 7 cut(s) 15, 406, 466, 516, 1091, 1136, 1717
BseGI GGATG 9 cut(s) 25, 122, 217, 372, 808, 1489, 1512, 1588, 1654
BseJI GATNNNNATC 2 cut(s) 8, 736
BseLI CCNNNNNNNGG 5 cut(s) 411, 644, 830, 1079, 1264
BseMII CTCAG 1 cut(s) 1104
BseNI ACTGG 2 cut(s) 666, 1046
BsePI GCGCGC 1 cut(s) 592
BseRI GAGGAG 1 cut(s) 1592
BseSI GKGCMC 1 cut(s) 924
BseXI GCAGC 4 cut(s) 127, 498, 1675, 1677
BseYI CCCAGC 1 cut(s) 548
BsgI GTGCAG 1 cut(s) 1461
Bsh1236I CGCG 3 cut(s) 525, 594, 596
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 6 cut(s) 384, 415, 471, 573, 1499, 1722
BshNI GGYRCC 1 cut(s) 365
BshVI ATCGAT 1 cut(s) 943
BsiEI CGRYCG 1 cut(s) 265
BsiSI CCGG 7 cut(s) 265, 296, 531, 914, 1136, 1559, 1699
BslFI GGGAC 2 cut(s) 1184, 1642
BslI CCNNNNNNNGG 5 cut(s) 411, 644, 830, 1079, 1264
BsmAI GTCTC 3 cut(s) 283, 494, 695
BsmBI CGTCTC 1 cut(s) 695
BsmFI GGGAC 2 cut(s) 1184, 1642
BsnI GGCC 6 cut(s) 384, 415, 471, 573, 1499, 1722
Bso31I GGTCTC 1 cut(s) 494
Bsp1286I GDGCHC 1 cut(s) 924
Bsp19I CCATGG 2 cut(s) 15, 1717
BspANI GGCC 6 cut(s) 384, 415, 471, 573, 1499, 1722
BspCNI CTCAG 1 cut(s) 1105
BspDI ATCGAT 1 cut(s) 943
BspFNI CGCG 3 cut(s) 525, 594, 596
BspLI GGNNCC 3 cut(s) 367, 1314, 1704
BspMAI CTGCAG 1 cut(s) 1505
BspPI GGATC 6 cut(s) 1052, 1257, 1289, 1307, 1399, 1467
BspT107I GGYRCC 1 cut(s) 365
BspTI CTTAAG 1 cut(s) 1289
BspTNI GGTCTC 1 cut(s) 494
BsrI ACTGG 2 cut(s) 666, 1046
BssECI CCNNGG 7 cut(s) 15, 406, 466, 516, 1091, 1136, 1717
BssHII GCGCGC 1 cut(s) 592
BssSI CACGAG 1 cut(s) 793
BssT1I CCWWGG 4 cut(s) 15, 406, 466, 1717
Bst2BI CACGAG 1 cut(s) 793
Bst2UI CCWGG 2 cut(s) 605, 1093
Bst4CI ACNGT 5 cut(s) 365, 517, 689, 1009, 1221
Bst6I CTCTTC 1 cut(s) 1730
BstAFI CTTAAG 1 cut(s) 1289
BstBAI YACGTR 2 cut(s) 1363, 1594
BstC8I GCNNGC 4 cut(s) 329, 443, 594, 1501
BstDEI CTNAG 4 cut(s) 1074, 1113, 1632, 1639
BstDSI CCRYGG 3 cut(s) 15, 516, 1717
BstEII GGTNACC 1 cut(s) 298
BstF5I GGATG 9 cut(s) 25, 122, 217, 372, 808, 1489, 1512, 1588, 1654
BstFNI CGCG 3 cut(s) 525, 594, 596
BstHHI GCGC 5 cut(s) 525, 594, 596, 700, 939
BstMAI GTCTC 3 cut(s) 283, 494, 695
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 4 cut(s) 146, 570, 605, 1719
BstNI CCWGG 2 cut(s) 605, 1093
BstNSI RCATGY 1 cut(s) 445
BstPI GGTNACC 1 cut(s) 298
BstSCI CCNGG 5 cut(s) 603, 912, 1091, 1135, 1698
BstSFI CTRYAG 3 cut(s) 144, 853, 1501
BstSLI GKGCMC 1 cut(s) 924
BstUI CGCG 3 cut(s) 525, 594, 596
BstV1I GCAGC 4 cut(s) 127, 498, 1675, 1677
BstV2I GAAGAC 1 cut(s) 1625
BstX2I RGATCY 3 cut(s) 1249, 1391, 1459
BstYI RGATCY 3 cut(s) 1249, 1391, 1459
Bsu15I ATCGAT 1 cut(s) 943
BsuRI GGCC 6 cut(s) 384, 415, 471, 573, 1499, 1722
BsuTUI ATCGAT 1 cut(s) 943
BtgI CCRYGG 3 cut(s) 15, 516, 1717
BtgZI GCGATG 1 cut(s) 206
BtrI CACGTC 1 cut(s) 919
BtsCI GGATG 9 cut(s) 25, 122, 217, 372, 808, 1489, 1512, 1588, 1654
BtsIMutI CAGTG 1 cut(s) 1014
Cac8I GCNNGC 4 cut(s) 329, 443, 594, 1501
CfoI GCGC 5 cut(s) 525, 594, 596, 700, 939
Cfr13I GGNCC 2 cut(s) 383, 1514
ClaI ATCGAT 1 cut(s) 943
Csp6I GTAC 7 cut(s) 134, 1085, 1096, 1217, 1226, 1510, 1591
CviQI GTAC 7 cut(s) 134, 1085, 1096, 1217, 1226, 1510, 1591
DdeI CTNAG 4 cut(s) 1074, 1113, 1632, 1639
Eam1104I CTCTTC 1 cut(s) 1730
EarI CTCTTC 1 cut(s) 1730
Eco130I CCWWGG 4 cut(s) 15, 406, 466, 1717
Eco31I GGTCTC 1 cut(s) 494
Eco32I GATATC 1 cut(s) 536
Eco47I GGWCC 1 cut(s) 1514
Eco57I CTGAAG 2 cut(s) 1422, 1706
Eco91I GGTNACC 1 cut(s) 298
EcoO65I GGTNACC 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 1337
EcoRII CCWGG 2 cut(s) 603, 1091
EcoRV GATATC 1 cut(s) 536
EcoT14I CCWWGG 4 cut(s) 15, 406, 466, 1717
EcoT22I ATGCAT 1 cut(s) 1168
ErhI CCWWGG 4 cut(s) 15, 406, 466, 1717
Esp3I CGTCTC 1 cut(s) 695
FaqI GGGAC 2 cut(s) 1184, 1642
FauI CCCGC 2 cut(s) 1668, 1703
FbaI TGATCA 3 cut(s) 1026, 1177, 1347
Fnu4HI GCNGC 9 cut(s) 141, 413, 487, 490, 597, 767, 1664, 1680, 1691
FokI GGATG 9 cut(s) 32, 109, 224, 359, 795, 1496, 1519, 1595, 1661
Fsp4HI GCNGC 9 cut(s) 141, 413, 487, 490, 597, 767, 1664, 1680, 1691
FspBI CTAG 6 cut(s) 111, 137, 272, 1334, 1463, 1551
GlaI GCGC 5 cut(s) 524, 593, 595, 699, 938
GluI GCNGC 9 cut(s) 141, 413, 487, 490, 597, 767, 1664, 1680, 1691
GsaI CCCAGC 1 cut(s) 552
GsuI CTGGAG 1 cut(s) 1029
HaeIII GGCC 6 cut(s) 384, 415, 471, 573, 1499, 1722
HapII CCGG 7 cut(s) 265, 296, 531, 914, 1136, 1559, 1699
HhaI GCGC 5 cut(s) 525, 594, 596, 700, 939
Hin6I GCGC 5 cut(s) 523, 592, 594, 698, 937
HinP1I GCGC 5 cut(s) 523, 592, 594, 698, 937
HincII GTYRAC 2 cut(s) 994, 1105
HindII GTYRAC 2 cut(s) 994, 1105
HpaII CCGG 7 cut(s) 265, 296, 531, 914, 1136, 1559, 1699
HphI GGTGA 5 cut(s) 292, 868, 961, 1462, 1544
Hpy166II GTNNAC 2 cut(s) 994, 1105
Hpy188I TCNGA 8 cut(s) 78, 283, 712, 1082, 1114, 1343, 1402, 1686
Hpy188III TCNNGA 9 cut(s) 199, 209, 457, 499, 1181, 1303, 1334, 1463, 1729
Hpy8I GTNNAC 2 cut(s) 994, 1105
Hpy99I CGWCG 2 cut(s) 440, 500
HpyAV CCTTC 6 cut(s) 126, 264, 410, 725, 1726, 1754
HpyCH4III ACNGT 5 cut(s) 365, 517, 689, 1009, 1221
HpyCH4IV ACGT 7 cut(s) 495, 918, 1233, 1362, 1493, 1572, 1593
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 570, 605, 1719
HpyF3I CTNAG 4 cut(s) 1074, 1113, 1632, 1639
HpySE526I ACGT 7 cut(s) 495, 918, 1233, 1362, 1493, 1572, 1593
HspAI GCGC 5 cut(s) 523, 592, 594, 698, 937
Ksp22I TGATCA 3 cut(s) 1026, 1177, 1347
LmnI GCTCC 3 cut(s) 178, 298, 520
Lsp1109I GCAGC 4 cut(s) 127, 498, 1675, 1677
LweI GCATC 5 cut(s) 10, 561, 883, 1271, 1639
MaeI CTAG 6 cut(s) 111, 137, 272, 1334, 1463, 1551
MaeII ACGT 7 cut(s) 495, 918, 1233, 1362, 1493, 1572, 1593
MaeIII GTNAC 2 cut(s) 298, 1303
MboII GAAGA 6 cut(s) 24, 239, 1196, 1415, 1630, 1717
MflI RGATCY 3 cut(s) 1249, 1391, 1459
MhlI GDGCHC 1 cut(s) 924
MluCI AATT 7 cut(s) 48, 243, 286, 395, 1337, 1519, 1762
MlyI GAGTC 2 cut(s) 716, 1118
MmeI TCCRAC 2 cut(s) 306, 1500
Mph1103I ATGCAT 1 cut(s) 1168
MseI TTAA 3 cut(s) 1020, 1161, 1290
MslI CAYNNNNRTG 5 cut(s) 797, 891, 1001, 1022, 1419
MspA1I CMGCKG 1 cut(s) 631
MspCI CTTAAG 1 cut(s) 1289
MspI CCGG 7 cut(s) 265, 296, 531, 914, 1136, 1559, 1699
MspR9I CCNGG 5 cut(s) 605, 914, 1093, 1137, 1700
MvaI CCWGG 2 cut(s) 605, 1093
MvnI CGCG 3 cut(s) 525, 594, 596
MwoI GCNNNNNNNGC 4 cut(s) 146, 570, 605, 1719
NciI CCSGG 3 cut(s) 914, 1137, 1700
NcoI CCATGG 2 cut(s) 15, 1717
NlaIV GGNNCC 3 cut(s) 367, 1314, 1704
NmeAIII GCCGAG 1 cut(s) 497
NmuCI GTSAC 2 cut(s) 298, 1303
NsiI ATGCAT 1 cut(s) 1168
NspI RCATGY 1 cut(s) 445
PaeI GCATGC 1 cut(s) 445
PauI GCGCGC 1 cut(s) 592
PcsI WCGNNNNNNNCGW 1 cut(s) 1104
PfeI GAWTC 8 cut(s) 615, 634, 1012, 1330, 1406, 1424, 1564, 1628
PkrI GCNGC 9 cut(s) 142, 414, 488, 491, 598, 768, 1665, 1681, 1692
PleI GAGTC 2 cut(s) 715, 1117
PpsI GAGTC 2 cut(s) 715, 1117
Ppu21I YACGTR 2 cut(s) 1363, 1594
PshBI ATTAAT 1 cut(s) 1161
PsiI TTATAA 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 603, 1091
PspEI GGTNACC 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 2 cut(s) 603, 1091
PspN4I GGNNCC 3 cut(s) 367, 1314, 1704
PspPI GGNCC 2 cut(s) 383, 1514
PstI CTGCAG 1 cut(s) 1505
PsuI RGATCY 3 cut(s) 1249, 1391, 1459
PteI GCGCGC 1 cut(s) 592
PvuII CAGCTG 1 cut(s) 631
RsaI GTAC 7 cut(s) 135, 1086, 1097, 1218, 1227, 1511, 1592
RsaNI GTAC 7 cut(s) 134, 1085, 1096, 1217, 1226, 1510, 1591
RseI CAYNNNNRTG 5 cut(s) 797, 891, 1001, 1022, 1419
SaqAI TTAA 3 cut(s) 1020, 1161, 1290
SatI GCNGC 9 cut(s) 141, 413, 487, 490, 597, 767, 1664, 1680, 1691
Sau96I GGNCC 2 cut(s) 383, 1514
SbfI CCTGCAGG 1 cut(s) 1505
ScaI AGTACT 1 cut(s) 1086
SchI GAGTC 2 cut(s) 716, 1118
ScrFI CCNGG 5 cut(s) 605, 914, 1093, 1137, 1700
SdaI CCTGCAGG 1 cut(s) 1505
SduI GDGCHC 1 cut(s) 924
SfaNI GCATC 5 cut(s) 10, 561, 883, 1271, 1639
SfcI CTRYAG 3 cut(s) 144, 853, 1501
SinI GGWCC 1 cut(s) 1514
SmiMI CAYNNNNRTG 5 cut(s) 797, 891, 1001, 1022, 1419
SmlI CTYRAG 1 cut(s) 1289
SmoI CTYRAG 1 cut(s) 1289
SpeI ACTAGT 1 cut(s) 110
SphI GCATGC 1 cut(s) 445
Sse8387I CCTGCAGG 1 cut(s) 1505
Sse9I AATT 7 cut(s) 48, 243, 286, 395, 1337, 1519, 1762
SspMI CTAG 6 cut(s) 111, 137, 272, 1334, 1463, 1551
StyD4I CCNGG 5 cut(s) 603, 912, 1091, 1135, 1698
StyI CCWWGG 4 cut(s) 15, 406, 466, 1717
TaaI ACNGT 5 cut(s) 365, 517, 689, 1009, 1221
TaiI ACGT 7 cut(s) 498, 921, 1236, 1365, 1496, 1575, 1596
TaqI TCGA 9 cut(s) 250, 261, 429, 498, 613, 740, 777, 891, 943
TasI AATT 7 cut(s) 48, 243, 286, 395, 1337, 1519, 1762
TatI WGTACW 2 cut(s) 1084, 1216
TauI GCSGC 5 cut(s) 415, 492, 599, 769, 1682
TfiI GAWTC 8 cut(s) 615, 634, 1012, 1330, 1406, 1424, 1564, 1628
Tru1I TTAA 3 cut(s) 1020, 1161, 1290
Tru9I TTAA 3 cut(s) 1020, 1161, 1290
TscAI CASTG 1 cut(s) 1014
TseFI GTSAC 2 cut(s) 298, 1303
TseI GCWGC 4 cut(s) 140, 486, 1663, 1690
Tsp45I GTSAC 2 cut(s) 298, 1303
TspDTI ATGAA 2 cut(s) 208, 1500
TspGWI ACGGA 5 cut(s) 759, 915, 1213, 1247, 1527
TspRI CASTG 1 cut(s) 1014
Vha464I CTTAAG 1 cut(s) 1289
VpaK11BI GGWCC 1 cut(s) 1514
VspI ATTAAT 1 cut(s) 1161
XapI RAATTY 1 cut(s) 1337
XbaI TCTAGA 2 cut(s) 1333, 1462
XceI RCATGY 1 cut(s) 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 428
XspI CTAG 6 cut(s) 111, 137, 272, 1334, 1463, 1551
ZrmI AGTACT 1 cut(s) 1086
Zsp2I ATGCAT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.