Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0016334

Physical Location & Seq

Genomic Coordinates Reverse (-)

25090162 .. 25093513

Gene Structure 3352 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1112400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGGCGATCGCATCTCCATCCCCTCCGACCTTCTCCGCCTCCTTCTGCCGCACCCGTACCGAGCCTCCCTCCACCTTCACTCGCATCCCTTCCATTTTCTCCCGCGCTCACCGACCCAAGCCTCACCCCCTGACTCGCCGGCTTGCCCTTTCCGCTCGGCTCAACTCGTCCATATCTTCCGCTGCCGGCGGCTCGGTCTCGCCGGACAACGGCGACCTCCAGTACGAGCTCTTCCACGGCTTCTCGCAGCAGCGTCGCCGGAGAGGATCGCCGGTGTTCGTCACGCTGCCTGTGGATTCCGTGGGGTCACGGGGCGCAGTCCGGAGGCCCAAGGCCATGGTTCAGTCGTTGAAGGCCCTCGCCGCGGCCGGAGTGGAAGGCGTGGTCATGGAGGTGTGGTGGGGCCTGGTGGAGAGGGATCAGCCTATGCTTTACAATTGGGAAGGCTACTTGGAGATCGTATCTCTGGCTCGACGCTGCGGGTTGAAGGTTCGGGCCGTCTTGGCGTTTCATCAGTGCGGCACTGGGCCGGAGGACTCTCATTGGATTCCTCTTCCTCTGTGGGTACTGGAGGAGATTGATAGGGATCCAGATTTAGTATATTCGGACAGATTTGGAAGAAGAAATGTGGAATACCTTTCTCTTGGGTGTGATATGCTTCCTGTTCTGCGGGGACGTTCACCAGTCCAAGCATATGCAGATTTTATGAGGAACTTCAGAGACACTTTTAGACCTTTTTTCGGTCTTGTCATAACAGGAATTCAAGTTGGGATGGGTCCTGCTGGTGAATTAAGATACCCTTCCTGCCCTACCCAGAAGTTAACATGGGCTTGGCGATCTCGTGAGCTTGGAGAGTTTCAGTGTTATGACAAGTATATGCTTGCATCTCTCAATGCATGTGCAAGAGAGATTGGGATTCCCGAATGGGGAAATGGGGGCCCTATTGGTGCTGCCAATTTGATGCAGGATCCTGAACTGACAGAGTTCTTCAGAAGTGATGATGGATCGTGGAATACATCATATGGTACATTTTTTCTTGAATGGTACTCGGGAATGCTGCTTCTGCATGGAGAAAGGATATGCAGGGAAGCCGAGACCATTTTCCGGGGTACAGAGGCTAGTACATCAGTAAAATTAGCTGGGATGCACTGGCATTATCGCACTAAATCTCACCCATCAGAGTTAACAGCTGGCTATTTCAATACTTCAACAAGAGACGGATACTTACCAATTGTTCGCATGTTGGCAAAGTACGGCTTTACTTTGTGCTGCTCATGTTTTGAATTGCAAGATGTGGAAGAGAGGAAGATGAACGCAGTCAGCAGCCCGGAAAGCCTTCTCAGACAGCTGCTCTCAGCTGCTAGGGTATGTGATATACCCTTGGAAGGTGAAACTTCTGCAGCCAGATTGGACAGCCAATCGCTTCAACAGGTGGTAAGGATGTCAAAGTATTACTCATCTGGTCTAGAAAAACCCTCTTTTTCGTTCAACTTTGTGAGGATGGACAAGAACATGTTCGAATTTCATAACTGGGTCCATTTTACTCGTTTTGTGAGGCAAATGTCTGATGCCAACGCCTTTCGAGCCAAGCTGGAAGTTGATGCAATGCCTGATACACGTCGGTCTCCCACTTCCCTTTCAGATGTTGCGAAATTTGGAATGGCTTCTGCATATTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016052 GO:0016160 GO:0016161 GO:0016787 GO:0016798 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_14

Protein Analysis

560

Amino Acids

63.03

Weight (kDa)

8.71

Isoelectric Point (pI)

50.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_14	PF01373	93 - 516	3.6e-121	Glycosyl hydrolase family 14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016334)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g11520
malus_domestica	MD06G1112400.v1.1 MD14G1133900.v1.1
prunus_persica	Prupe.5G125700_v2.0.a1
pyrus_communis	pycom06g10540
rosa_chinensis	RchiOBHm_Chr7g0186231
rosa_laevigata	RLG00000004855
rosa_multiflora	Rmu_sc0002609.1_g000004
rosa_roxburghii	Rroxscaffold_3G00268290
rosa_rugosa	Rorug06G0472800
rosa_samantha	Rh7AG068400 Rh7BG084100 Rh7CG079700 Rh7DG080200
rosa_wichuraiana	Rw7G006670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	155
AccII	CGCG	2 cut(s)	105, 365
AccIII	TCCGGA	1 cut(s)	321
AclWI	GGATC	7 cut(s)	274, 426, 581, 594, 962, 975, 1012
AcoI	YGGCCR	1 cut(s)	366
AcsI	RAATTY	3 cut(s)	759, 1520, 1652
AcuI	CTGAAG	2 cut(s)	700, 973
AfaI	GTAC	8 cut(s)	58, 224, 567, 1027, 1046, 1111, 1123, 1253
AfiI	CCNNNNNNNGG	6 cut(s)	209, 364, 740, 926, 1104, 1385
AflIII	ACRYGT	2 cut(s)	1512, 1616
AgsI	TTSAA	9 cut(s)	352, 487, 764, 1040, 1201, 1209, 1283, 1427, 1489
AjiI	CACGTC	1 cut(s)	1619
AjnI	CCWGG	1 cut(s)	405
AluBI	AGCT	7 cut(s)	229, 847, 1139, 1190, 1348, 1358, 1591
AluI	AGCT	7 cut(s)	229, 847, 1139, 1190, 1348, 1358, 1591
Alw21I	GWGCWC	1 cut(s)	231
Alw26I	GTCTC	5 cut(s)	202, 714, 1088, 1209, 1629
AlwI	GGATC	7 cut(s)	274, 426, 581, 594, 962, 975, 1012
Ama87I	CYCGRG	1 cut(s)	1048
Aor13HI	TCCGGA	1 cut(s)	321
AoxI	GGCC	8 cut(s)	326, 333, 354, 366, 403, 495, 527, 937
ApaI	GGGCCC	1 cut(s)	941
ApoI	RAATTY	3 cut(s)	759, 1520, 1652
AsiSI	GCGATCGC	1 cut(s)	9
Asp700I	GAANNNNTTC	3 cut(s)	1335, 1514, 1663
AspLEI	GCGC	2 cut(s)	107, 317
AspS9I	GGNCC	9 cut(s)	327, 355, 403, 495, 527, 776, 937, 938, 1534
AsuC2I	CCSGG	2 cut(s)	1106, 1328
AsuHPI	GGTGA	6 cut(s)	101, 116, 672, 797, 1163, 1400
AsuII	TTCGAA	1 cut(s)	1518
AvaI	CYCGRG	1 cut(s)	1048
AvaII	GGWCC	2 cut(s)	776, 1534
BaeGI	GKGCMC	1 cut(s)	941
BamHI	GGATCC	2 cut(s)	586, 967
BanII	GRGCYC	2 cut(s)	231, 941
BarI	GAAGNNNNNNTAC	2 cut(s)	215, 247
BauI	CACGAG	1 cut(s)	840
Bbv12I	GWGCWC	1 cut(s)	231
BccI	CCATC	5 cut(s)	25, 766, 995, 1183, 1495
BceAI	ACGGC	4 cut(s)	226, 253, 482, 1270
BcgI	CGANNNNNNTGC	2 cut(s)	236, 270
BciT130I	CCWGG	1 cut(s)	407
BciVI	GTATCC	1 cut(s)	1214
BcnI	CCSGG	2 cut(s)	1106, 1328
BcoDI	GTCTC	5 cut(s)	202, 714, 1088, 1209, 1629
BfaI	CTAG	3 cut(s)	1119, 1362, 1466
BfmI	CTRYAG	1 cut(s)	1398
BfuI	GTATCC	1 cut(s)	1214
BglI	GCCNNNNNGGC	1 cut(s)	503
Bme1390I	CCNGG	3 cut(s)	407, 1106, 1328
Bme18I	GGWCC	2 cut(s)	776, 1534
BmeT110I	CYCGRG	1 cut(s)	1048
BmgBI	CACGTC	1 cut(s)	1619
BmgT120I	GGNCC	9 cut(s)	327, 355, 403, 495, 527, 776, 937, 938, 1534
BmiI	GGNNCC	7 cut(s)	404, 588, 777, 938, 939, 969, 1535
BmrFI	CCNGG	3 cut(s)	407, 1106, 1328
BmrI	ACTGGG	2 cut(s)	534, 1540
BmsI	GCATC	7 cut(s)	20, 93, 893, 951, 1134, 1558, 1591
BmuI	ACTGGG	2 cut(s)	534, 1540
BplI	GAGNNNNNCTC	2 cut(s)	53, 85
BpmI	CTGGAG	2 cut(s)	203, 590
Bpu14I	TTCGAA	1 cut(s)	1518
BpuMI	CCSGG	2 cut(s)	1106, 1328
BsaBI	GATNNNNATC	1 cut(s)	585
BsaI	GGTCTC	3 cut(s)	202, 1088, 1629
BsaJI	CCNNGG	7 cut(s)	235, 300, 330, 336, 363, 1105, 1380
BsaWI	WCCGGW	1 cut(s)	321
BsaXI	ACNNNNNCTCC	4 cut(s)	49, 79, 383, 413
Bsc4I	CCNNNNNNNGG	6 cut(s)	209, 364, 740, 926, 1104, 1385
Bse118I	RCCGGY	3 cut(s)	138, 185, 271
Bse1I	ACTGG	6 cut(s)	220, 529, 573, 683, 1154, 1535
Bse3DI	GCAATG	1 cut(s)	1611
Bse8I	GATNNNNATC	1 cut(s)	585
BseAI	TCCGGA	1 cut(s)	321
BseBI	CCWGG	1 cut(s)	407
BseDI	CCNNGG	7 cut(s)	235, 300, 330, 336, 363, 1105, 1380
BseGI	GGATG	6 cut(s)	17, 84, 777, 1149, 1446, 1506
BseJI	GATNNNNATC	1 cut(s)	585
BseLI	CCNNNNNNNGG	6 cut(s)	209, 364, 740, 926, 1104, 1385
BseMI	GCAATG	1 cut(s)	1611
BseMII	CTCAG	2 cut(s)	1354, 1368
BseNI	ACTGG	6 cut(s)	220, 529, 573, 683, 1154, 1535
BseRI	GAGGAG	1 cut(s)	587
BseSI	GKGCMC	1 cut(s)	941
BseX3I	CGGCCG	1 cut(s)	366
BseYI	CCCAGC	1 cut(s)	1139
Bsh1236I	CGCG	2 cut(s)	105, 365
Bsh1285I	CGRYCG	2 cut(s)	9, 369
BshFI	GGCC	8 cut(s)	328, 335, 356, 368, 405, 497, 529, 939
BsiEI	CGRYCG	2 cut(s)	9, 369
BsiHKAI	GWGCWC	1 cut(s)	231
BsiHKCI	CYCGRG	1 cut(s)	1048
BslFI	GGGAC	1 cut(s)	687
BslI	CCNNNNNNNGG	6 cut(s)	209, 364, 740, 926, 1104, 1385
BsmAI	GTCTC	5 cut(s)	202, 714, 1088, 1209, 1629
BsmBI	CGTCTC	1 cut(s)	1209
BsmFI	GGGAC	1 cut(s)	687
BsmI	GAATGC	1 cut(s)	1059
BsnI	GGCC	8 cut(s)	328, 335, 356, 368, 405, 497, 529, 939
Bso31I	GGTCTC	3 cut(s)	202, 1088, 1629
BsoBI	CYCGRG	1 cut(s)	1048
Bsp119I	TTCGAA	1 cut(s)	1518
Bsp120I	GGGCCC	1 cut(s)	937
Bsp1286I	GDGCHC	2 cut(s)	231, 941
Bsp13I	TCCGGA	1 cut(s)	321
Bsp143I	GATC	8 cut(s)	6, 266, 418, 456, 586, 836, 967, 1004
Bsp19I	CCATGG	1 cut(s)	336
BspANI	GGCC	8 cut(s)	328, 335, 356, 368, 405, 497, 529, 939
BspCNI	CTCAG	2 cut(s)	1353, 1367
BspEI	TCCGGA	1 cut(s)	321
BspFNI	CGCG	2 cut(s)	105, 365
BspLI	GGNNCC	7 cut(s)	404, 588, 777, 938, 939, 969, 1535
BspMAI	CTGCAG	1 cut(s)	1402
BspPI	GGATC	7 cut(s)	274, 426, 581, 594, 962, 975, 1012
BspQI	GCTCTTC	1 cut(s)	236
BspT104I	TTCGAA	1 cut(s)	1518
BspTNI	GGTCTC	3 cut(s)	202, 1088, 1629
BsrBI	CCGCTC	1 cut(s)	155
BsrDI	GCAATG	1 cut(s)	1611
BsrFI	RCCGGY	3 cut(s)	138, 185, 271
BsrI	ACTGG	6 cut(s)	220, 529, 573, 683, 1154, 1535
BssAI	RCCGGY	3 cut(s)	138, 185, 271
BssECI	CCNNGG	7 cut(s)	235, 300, 330, 336, 363, 1105, 1380
BssMI	GATC	8 cut(s)	6, 266, 418, 456, 586, 836, 967, 1004
BssSI	CACGAG	1 cut(s)	840
BssT1I	CCWWGG	3 cut(s)	330, 336, 1380
Bst2BI	CACGAG	1 cut(s)	840
Bst2UI	CCWGG	1 cut(s)	407
Bst6I	CTCTTC	3 cut(s)	236, 558, 1293
BstBI	TTCGAA	1 cut(s)	1518
BstC8I	GCNNGC	5 cut(s)	140, 144, 187, 882, 1192
BstDEI	CTNAG	2 cut(s)	1340, 1354
BstDSI	CCRYGG	4 cut(s)	235, 300, 336, 363
BstF5I	GGATG	6 cut(s)	17, 84, 777, 1149, 1446, 1506
BstFNI	CGCG	2 cut(s)	105, 365
BstHHI	GCGC	2 cut(s)	107, 317
BstKTI	GATC	8 cut(s)	9, 269, 421, 459, 589, 839, 970, 1007
BstMAI	GTCTC	5 cut(s)	202, 714, 1088, 1209, 1629
BstMBI	GATC	8 cut(s)	6, 266, 418, 456, 586, 836, 967, 1004
BstMCI	CGRYCG	2 cut(s)	9, 369
BstMWI	GCNNNNNNNGC	6 cut(s)	152, 362, 503, 1063, 1332, 1583
BstNI	CCWGG	1 cut(s)	407
BstNSI	RCATGY	3 cut(s)	900, 1243, 1516
BstSCI	CCNGG	3 cut(s)	405, 1104, 1326
BstSFI	CTRYAG	1 cut(s)	1398
BstSLI	GKGCMC	1 cut(s)	941
BstUI	CGCG	2 cut(s)	105, 365
BstX2I	RGATCY	2 cut(s)	586, 967
BstXI	CCANNNNNNTGG	1 cut(s)	337
BstYI	RGATCY	2 cut(s)	586, 967
BstZI	CGGCCG	1 cut(s)	366
BsuI	GTATCC	1 cut(s)	1214
BsuRI	GGCC	8 cut(s)	328, 335, 356, 368, 405, 497, 529, 939
BtgI	CCRYGG	4 cut(s)	235, 300, 336, 363
BtrI	CACGTC	1 cut(s)	1619
BtsCI	GGATG	6 cut(s)	17, 84, 777, 1149, 1446, 1506
BtsIMutI	CAGTG	4 cut(s)	521, 522, 866, 1147
Cac8I	GCNNGC	5 cut(s)	140, 144, 187, 882, 1192
CfoI	GCGC	2 cut(s)	107, 317
Cfr10I	RCCGGY	3 cut(s)	138, 185, 271
Cfr13I	GGNCC	9 cut(s)	327, 355, 403, 495, 527, 776, 937, 938, 1534
Cfr42I	CCGCGG	1 cut(s)	366
CseI	GACGC	2 cut(s)	242, 483
Csp6I	GTAC	8 cut(s)	57, 223, 566, 1026, 1045, 1110, 1122, 1252
CviAII	CATG	8 cut(s)	337, 388, 825, 897, 1067, 1240, 1275, 1513
CviQI	GTAC	8 cut(s)	57, 223, 566, 1026, 1045, 1110, 1122, 1252
DdeI	CTNAG	2 cut(s)	1340, 1354
DpnI	GATC	8 cut(s)	8, 268, 420, 458, 588, 838, 969, 1006
DpnII	GATC	8 cut(s)	6, 266, 418, 456, 586, 836, 967, 1004
EaeI	YGGCCR	1 cut(s)	366
EagI	CGGCCG	1 cut(s)	366
Eam1104I	CTCTTC	3 cut(s)	236, 558, 1293
EarI	CTCTTC	3 cut(s)	236, 558, 1293
EciI	GGCGGA	1 cut(s)	25
Ecl136II	GAGCTC	1 cut(s)	229
EclXI	CGGCCG	1 cut(s)	366
Eco130I	CCWWGG	3 cut(s)	330, 336, 1380
Eco24I	GRGCYC	2 cut(s)	231, 941
Eco31I	GGTCTC	3 cut(s)	202, 1088, 1629
Eco47I	GGWCC	2 cut(s)	776, 1534
Eco52I	CGGCCG	1 cut(s)	366
Eco53kI	GAGCTC	1 cut(s)	229
Eco57I	CTGAAG	2 cut(s)	700, 973
Eco88I	CYCGRG	1 cut(s)	1048
EcoICRI	GAGCTC	1 cut(s)	229
EcoO109I	RGGNCCY	5 cut(s)	355, 403, 776, 937, 938
EcoRI	GAATTC	1 cut(s)	759
EcoRII	CCWGG	1 cut(s)	405
EcoT14I	CCWWGG	3 cut(s)	330, 336, 1380
EcoT22I	ATGCAT	1 cut(s)	898
EcoT38I	GRGCYC	2 cut(s)	231, 941
ErhI	CCWWGG	3 cut(s)	330, 336, 1380
Esp3I	CGTCTC	1 cut(s)	1209
FaeI	CATG	8 cut(s)	340, 391, 828, 900, 1070, 1243, 1278, 1516
FalI	AAGNNNNNCTT	4 cut(s)	784, 816, 1241, 1273
FaqI	GGGAC	1 cut(s)	687
FatI	CATG	8 cut(s)	336, 387, 824, 896, 1066, 1239, 1274, 1512
FauI	CCCGC	3 cut(s)	110, 473, 663
FauNDI	CATATG	2 cut(s)	694, 1021
FokI	GGATG	6 cut(s)	4, 71, 784, 1156, 1453, 1513
FriOI	GRGCYC	2 cut(s)	231, 941
FspBI	CTAG	3 cut(s)	1119, 1362, 1466
GlaI	GCGC	2 cut(s)	106, 316
GsaI	CCCAGC	1 cut(s)	1143
GsuI	CTGGAG	2 cut(s)	203, 590
HaeIII	GGCC	8 cut(s)	328, 335, 356, 368, 405, 497, 529, 939
HgaI	GACGC	2 cut(s)	242, 483
HhaI	GCGC	2 cut(s)	107, 317
Hin1II	CATG	8 cut(s)	340, 391, 828, 900, 1070, 1243, 1278, 1516
Hin6I	GCGC	2 cut(s)	105, 315
HinP1I	GCGC	2 cut(s)	105, 315
HincII	GTYRAC	2 cut(s)	822, 1185
HindII	GTYRAC	2 cut(s)	822, 1185
HinfI	GANTC	5 cut(s)	133, 296, 536, 547, 916
HpaI	GTTAAC	2 cut(s)	822, 1185
HphI	GGTGA	6 cut(s)	101, 116, 672, 797, 1163, 1400
Hpy166II	GTNNAC	3 cut(s)	680, 822, 1185
Hpy188I	TCNGA	8 cut(s)	27, 607, 719, 992, 1180, 1343, 1567, 1642
Hpy188III	TCNNGA	8 cut(s)	322, 590, 842, 920, 971, 1037, 1050, 1466
Hpy8I	GTNNAC	3 cut(s)	680, 822, 1185
Hpy99I	CGWCG	3 cut(s)	258, 477, 1623
HpyCH4IV	ACGT	2 cut(s)	676, 1618
HpyF10VI	GCNNNNNNNGC	6 cut(s)	152, 362, 503, 1063, 1332, 1583
HpyF3I	CTNAG	2 cut(s)	1340, 1354
HpySE526I	ACGT	2 cut(s)	676, 1618
Hsp92II	CATG	8 cut(s)	340, 391, 828, 900, 1070, 1243, 1278, 1516
HspAI	GCGC	2 cut(s)	105, 315
Kpn2I	TCCGGA	1 cut(s)	321
KroI	GCCGGC	2 cut(s)	138, 185
KroNI	GCCGGC	2 cut(s)	140, 187
KspAI	GTTAAC	2 cut(s)	822, 1185
KspI	CCGCGG	1 cut(s)	366
Kzo9I	GATC	8 cut(s)	6, 266, 418, 456, 586, 836, 967, 1004
LguI	GCTCTTC	1 cut(s)	236
LweI	GCATC	7 cut(s)	20, 93, 893, 951, 1134, 1558, 1591
MaeI	CTAG	3 cut(s)	1119, 1362, 1466
MaeII	ACGT	2 cut(s)	676, 1618
MaeIII	GTNAC	2 cut(s)	280, 306
MalI	GATC	8 cut(s)	8, 268, 420, 458, 588, 838, 969, 1006
MbiI	CCGCTC	1 cut(s)	155
MboI	GATC	8 cut(s)	6, 266, 418, 456, 586, 836, 967, 1004
MboII	GAAGA	8 cut(s)	168, 223, 545, 630, 633, 979, 1310, 1318
MfeI	CAATTG	2 cut(s)	436, 1230
MflI	RGATCY	2 cut(s)	586, 967
MhlI	GDGCHC	2 cut(s)	231, 941
MluCI	AATT	9 cut(s)	436, 759, 788, 955, 1133, 1230, 1283, 1520, 1652
MlyI	GAGTC	2 cut(s)	127, 530
MmeI	TCCRAC	1 cut(s)	50
Mph1103I	ATGCAT	1 cut(s)	898
MroI	TCCGGA	1 cut(s)	321
MroNI	GCCGGC	2 cut(s)	138, 185
MroXI	GAANNNNTTC	3 cut(s)	1335, 1514, 1663
MseI	TTAA	3 cut(s)	791, 821, 1184
MslI	CAYNNNNRTG	1 cut(s)	392
MspA1I	CMGCKG	5 cut(s)	182, 365, 1190, 1348, 1358
MspR9I	CCNGG	3 cut(s)	407, 1106, 1328
MunI	CAATTG	2 cut(s)	436, 1230
Mva1269I	GAATGC	1 cut(s)	1059
MvaI	CCWGG	1 cut(s)	407
MvnI	CGCG	2 cut(s)	105, 365
MwoI	GCNNNNNNNGC	6 cut(s)	152, 362, 503, 1063, 1332, 1583
NaeI	GCCGGC	2 cut(s)	140, 187
NciI	CCSGG	2 cut(s)	1106, 1328
NcoI	CCATGG	1 cut(s)	336
NdeI	CATATG	2 cut(s)	694, 1021
NdeII	GATC	8 cut(s)	6, 266, 418, 456, 586, 836, 967, 1004
NgoMIV	GCCGGC	2 cut(s)	138, 185
NlaIII	CATG	8 cut(s)	340, 391, 828, 900, 1070, 1243, 1278, 1516
NlaIV	GGNNCC	7 cut(s)	404, 588, 777, 938, 939, 969, 1535
NmeAIII	GCCGAG	2 cut(s)	136, 1117
NmuCI	GTSAC	2 cut(s)	280, 306
NsiI	ATGCAT	1 cut(s)	898
NspI	RCATGY	3 cut(s)	900, 1243, 1516
NspV	TTCGAA	1 cut(s)	1518
PciI	ACATGT	1 cut(s)	1512
PciSI	GCTCTTC	1 cut(s)	236
PctI	GAATGC	1 cut(s)	1059
PdiI	GCCGGC	2 cut(s)	140, 187
PdmI	GAANNNNTTC	3 cut(s)	1335, 1514, 1663
PfeI	GAWTC	3 cut(s)	296, 547, 916
Ple19I	CGATCG	1 cut(s)	9
PleI	GAGTC	2 cut(s)	127, 530
PpsI	GAGTC	2 cut(s)	127, 530
PpuMI	RGGWCCY	1 cut(s)	776
PscI	ACATGT	1 cut(s)	1512
Psp124BI	GAGCTC	1 cut(s)	231
Psp5II	RGGWCCY	1 cut(s)	776
Psp6I	CCWGG	1 cut(s)	405
PspFI	CCCAGC	1 cut(s)	1139
PspGI	CCWGG	1 cut(s)	405
PspN4I	GGNNCC	7 cut(s)	404, 588, 777, 938, 939, 969, 1535
PspOMI	GGGCCC	1 cut(s)	937
PspPI	GGNCC	9 cut(s)	327, 355, 403, 495, 527, 776, 937, 938, 1534
PspPPI	RGGWCCY	1 cut(s)	776
PstI	CTGCAG	1 cut(s)	1402
PsuI	RGATCY	2 cut(s)	586, 967
PvuI	CGATCG	1 cut(s)	9
PvuII	CAGCTG	3 cut(s)	1190, 1348, 1358
RgaI	GCGATCGC	1 cut(s)	9
RsaI	GTAC	8 cut(s)	58, 224, 567, 1027, 1046, 1111, 1123, 1253
RsaNI	GTAC	8 cut(s)	57, 223, 566, 1026, 1045, 1110, 1122, 1252
RseI	CAYNNNNRTG	1 cut(s)	392
SacI	GAGCTC	1 cut(s)	231
SacII	CCGCGG	1 cut(s)	366
SapI	GCTCTTC	1 cut(s)	236
SaqAI	TTAA	3 cut(s)	791, 821, 1184
Sau3AI	GATC	8 cut(s)	6, 266, 418, 456, 586, 836, 967, 1004
Sau96I	GGNCC	9 cut(s)	327, 355, 403, 495, 527, 776, 937, 938, 1534
SchI	GAGTC	2 cut(s)	127, 530
ScrFI	CCNGG	3 cut(s)	407, 1106, 1328
SduI	GDGCHC	2 cut(s)	231, 941
SfaAI	GCGATCGC	1 cut(s)	9
SfaNI	GCATC	7 cut(s)	20, 93, 893, 951, 1134, 1558, 1591
SfcI	CTRYAG	1 cut(s)	1398
Sfr303I	CCGCGG	1 cut(s)	366
SfuI	TTCGAA	1 cut(s)	1518
SgfI	GCGATCGC	1 cut(s)	9
SgrAI	CRCCGGYG	1 cut(s)	271
SgrBI	CCGCGG	1 cut(s)	366
SinI	GGWCC	2 cut(s)	776, 1534
SmiMI	CAYNNNNRTG	1 cut(s)	392
Sse9I	AATT	9 cut(s)	436, 759, 788, 955, 1133, 1230, 1283, 1520, 1652
SspMI	CTAG	3 cut(s)	1119, 1362, 1466
SstI	GAGCTC	1 cut(s)	231
StyD4I	CCNGG	3 cut(s)	405, 1104, 1326
StyI	CCWWGG	3 cut(s)	330, 336, 1380
TaiI	ACGT	2 cut(s)	679, 1621
TaqI	TCGA	3 cut(s)	472, 1518, 1582
TaqII	GACCGA	3 cut(s)	184, 731, 1611
TasI	AATT	9 cut(s)	436, 759, 788, 955, 1133, 1230, 1283, 1520, 1652
TatI	WGTACW	1 cut(s)	1121
TauI	GCSGC	5 cut(s)	51, 192, 365, 368, 522
TfiI	GAWTC	3 cut(s)	296, 547, 916
Tru1I	TTAA	3 cut(s)	791, 821, 1184
Tru9I	TTAA	3 cut(s)	791, 821, 1184
TscAI	CASTG	4 cut(s)	521, 529, 866, 1154
TseFI	GTSAC	2 cut(s)	280, 306
Tsp45I	GTSAC	2 cut(s)	280, 306
TspDTI	ATGAA	3 cut(s)	500, 1325, 1514
TspGWI	ACGGA	2 cut(s)	289, 1233
TspRI	CASTG	4 cut(s)	521, 529, 866, 1154
VpaK11BI	GGWCC	2 cut(s)	776, 1534
XapI	RAATTY	3 cut(s)	759, 1520, 1652
XbaI	TCTAGA	1 cut(s)	1465
XceI	RCATGY	3 cut(s)	900, 1243, 1516
XmnI	GAANNNNTTC	3 cut(s)	1335, 1514, 1663
XspI	CTAG	3 cut(s)	1119, 1362, 1466
Zsp2I	ATGCAT	1 cut(s)	898

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.