Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0016334

Physical Location & Seq

Genomic Coordinates Reverse (-)

21216583 .. 21220024

Gene Structure 3442 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1133900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGGCGATCTCATCTCCACCAGTTCCGACCTTCTCCGCCTCCTTCTGCCGCACCCGCACCGAGTTTCCCTCCACCTTTAATCGCATCCCTTCCATTTTCTCCCGCGCTCACCGAGCCAAACCTCACCCCCTAACTCGCCGGCTCGCCCTCTCCGCTCGTCTCAACTCGTCCAAGTCTTCCGATGCCGGCGGCTCGGTCTCGCCGGACAACGGCGACCTCCAGTACGAGCTCTTCCACGGATTCTCGCCGCAGCGCTGCCGGAGAGGATCTCCGGTGTTCGTCACACTGCCTGTGCACTCCGTTGGGTCACTGGGCGCGGTTCGGAGGCCCAAGGCCATGGTCCAGTCGTTTAAGGCCCTGGCCGTGGCCGGAGTGGAAGGCGTGGTCATGGAGGTGTGGTGGGGTCTGGTGGAGAGGGATCGACCCATGCTTTACAATTGGGAAGGCTACTTGGAGATCGTCTCTCTGGCTCGACGCTGCGGATTGAAGGTTCGGGCCGTCTTGGCGTTTCATCAGTGCGGCGCTGGGCCGGAGGACTCTCATTGGATTCCTCTTCCTCTGTGGGTACTTGAGGAGATCGATAAGGATCCAGATTTAGTATATTCTGACAGATTTGGAAGAAGAAATGTCGAGTACCTTTCTCTCGGGTGTGATATGCTTCCTGTTCTGCGGGGACGTTCACCACTTCAAGCATATGCGGATTTTATGAGGAACTTCAGAGACACTTTCAGACCTTTTCTTGGTGTTGTCATAACAGGAATTCAAGTTGGGATGGGGCCTGCTGGTGAATTAAGATACCCTTCTTGCCCTACACAGAAGCTAACATGGGCTTGGCGATCTCGTGAGCTTGGAGAGTTTCAGTGCTATGACAAGTATATGCTTGCGTCTCTCAATGCATGTGCAAGAGAAATTGGGATGCCTGAATGGGGAAATGGAGGCCCTTTTGGTGCTGCCAATTTAATGCAGGATCCTGAACTGACAGAGTTCTTCAGAAGTGATGATGGATCCTGGAATACATCTTATGGTACTTTTTTTCTTGAATGGTACTCAGGAATGCTGGTTCTGCATGGTGAAAGGATGTGCAGGGAAGCTGAGACCATTTTCCGGGGTACAGAGGCTAGTACGTCAGTAAAGATAGCTGGGATGCACTGGCATTACCGCACTAAGTCTCATCCGTCAGAGTTAACAGCTGGCTATTACAATACTTCAACAAGAGATGGATACTTGCCAATTGCTCGCATGTTGGCAAAGTACGGCTTTACTTTGTGCTGCTCAGGTTTTGACTTGCAAGATCAGGAAGAGAGGAAGATGAACCCTGTCAGCAGCCCGGAAGGCCTTCTCAGACAGTTGCTTTCAGCTGCTAGGTTGTGTGATATACCACTGGAAGGTGAAACTTCTGCTGCCAGTTTGGACAATGAATCTTTGCAACAGGTGGTAAGGATGTCAAAGTATTACTCATATGGTCTAGAAAAGCCCTCGTTTTCGTTCAACTTTGTGAGGATGGACAAGAACATGTTCGAATTTCATAATTGGGTTCATTTTACTCGTTTTGTGAGACAAATGTCTGATGCCAATGCCTTTCGAGCCAAGCTAGAAGTTGACGCAATGCCTGATACACGTCGGTCTCCCACTTCCCTTTCAGATGTCACGAAATTTGGAATGGCTTCTGCATATTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016052 GO:0016160 GO:0016161 GO:0016787 GO:0016798 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_14

Protein Analysis

560

Amino Acids

63.13

Weight (kDa)

8.47

Isoelectric Point (pI)

47.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_14	PF01373	93 - 516	8.6e-117	Glycosyl hydrolase family 14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016334)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g11520
malus_domestica	MD06G1112400.v1.1 MD14G1133900.v1.1
prunus_persica	Prupe.5G125700_v2.0.a1
pyrus_communis	pycom06g10540
rosa_chinensis	RchiOBHm_Chr7g0186231
rosa_laevigata	RLG00000004855
rosa_multiflora	Rmu_sc0002609.1_g000004
rosa_roxburghii	Rroxscaffold_3G00268290
rosa_rugosa	Rorug06G0472800
rosa_samantha	Rh7AG068400 Rh7BG084100 Rh7CG079700 Rh7DG080200
rosa_wichuraiana	Rw7G006670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	155
AccII	CGCG	2 cut(s)	105, 317
AclWI	GGATC	8 cut(s)	274, 426, 581, 594, 962, 975, 999, 1012
AcoI	YGGCCR	2 cut(s)	360, 366
AcsI	RAATTY	3 cut(s)	759, 1520, 1652
AcuI	CTGAAG	2 cut(s)	700, 973
AfaI	GTAC	8 cut(s)	224, 567, 635, 1027, 1046, 1111, 1123, 1253
AfeI	AGCGCT	1 cut(s)	254
AfiI	CCNNNNNNNGG	6 cut(s)	209, 364, 740, 926, 1104, 1385
AflIII	ACRYGT	2 cut(s)	1512, 1616
AgsI	TTSAA	6 cut(s)	487, 689, 764, 1040, 1209, 1489
AjiI	CACGTC	1 cut(s)	1619
AjnI	CCWGG	2 cut(s)	357, 1007
AloI	GAACNNNNNNTCC	4 cut(s)	474, 506, 1044, 1076
AluBI	AGCT	8 cut(s)	229, 820, 847, 1091, 1139, 1190, 1358, 1591
AluI	AGCT	8 cut(s)	229, 820, 847, 1091, 1139, 1190, 1358, 1591
Alw21I	GWGCWC	2 cut(s)	231, 297
Alw26I	GTCTC	9 cut(s)	164, 202, 466, 714, 891, 1088, 1173, 1549, 1629
Alw44I	GTGCAC	1 cut(s)	293
AlwI	GGATC	8 cut(s)	274, 426, 581, 594, 962, 975, 999, 1012
Ama87I	CYCGRG	1 cut(s)	644
Aor51HI	AGCGCT	1 cut(s)	254
ApaLI	GTGCAC	1 cut(s)	293
ApeKI	GCWGC	8 cut(s)	250, 255, 477, 950, 1269, 1323, 1358, 1400
ApoI	RAATTY	3 cut(s)	759, 1520, 1652
Asp700I	GAANNNNTTC	3 cut(s)	1335, 1514, 1663
AspLEI	GCGC	4 cut(s)	107, 255, 317, 524
AspS9I	GGNCC	7 cut(s)	327, 340, 355, 495, 527, 776, 938
AsuC2I	CCSGG	2 cut(s)	1106, 1328
AsuHPI	GGTGA	6 cut(s)	101, 116, 672, 797, 1082, 1400
AsuII	TTCGAA	1 cut(s)	1518
AvaI	CYCGRG	1 cut(s)	644
AvaII	GGWCC	1 cut(s)	340
BaeGI	GKGCMC	1 cut(s)	297
BamHI	GGATCC	3 cut(s)	586, 967, 1004
BanII	GRGCYC	1 cut(s)	231
BarI	GAAGNNNNNNTAC	2 cut(s)	215, 247
BauI	CACGAG	1 cut(s)	840
BbsI	GAAGAC	1 cut(s)	168
Bbv12I	GWGCWC	2 cut(s)	231, 297
BbvI	GCAGC	8 cut(s)	242, 262, 464, 937, 1256, 1335, 1345, 1387
BccI	CCATC	4 cut(s)	766, 995, 1211, 1495
BceAI	ACGGC	4 cut(s)	226, 347, 482, 1270
BciT130I	CCWGG	2 cut(s)	359, 1009
BciVI	GTATCC	1 cut(s)	1214
BcnI	CCSGG	2 cut(s)	1106, 1328
BcoDI	GTCTC	9 cut(s)	164, 202, 466, 714, 891, 1088, 1173, 1549, 1629
BfaI	CTAG	5 cut(s)	1119, 1362, 1466, 1592, 1678
BfoI	RGCGCY	2 cut(s)	256, 525
BfuI	GTATCC	1 cut(s)	1214
BglI	GCCNNNNNGGC	2 cut(s)	503, 1332
Bme1390I	CCNGG	4 cut(s)	359, 1009, 1106, 1328
Bme18I	GGWCC	1 cut(s)	340
BmeT110I	CYCGRG	1 cut(s)	644
BmgBI	CACGTC	1 cut(s)	1619
BmgT120I	GGNCC	7 cut(s)	327, 340, 355, 495, 527, 776, 938
BmiI	GGNNCC	4 cut(s)	588, 777, 969, 1006
BmrFI	CCNGG	4 cut(s)	359, 1009, 1106, 1328
BmrI	ACTGGG	1 cut(s)	320
BmsI	GCATC	5 cut(s)	93, 172, 906, 1134, 1558
BmuI	ACTGGG	1 cut(s)	320
BoxI	GACNNNNGTC	1 cut(s)	1561
BpiI	GAAGAC	1 cut(s)	168
BplI	GAGNNNNNCTC	6 cut(s)	53, 85, 253, 285, 446, 478
BpmI	CTGGAG	1 cut(s)	203
Bpu10I	CCTNAGC	1 cut(s)	1273
Bpu14I	TTCGAA	1 cut(s)	1518
BpuEI	CTTGAG	1 cut(s)	590
BpuMI	CCSGG	2 cut(s)	1106, 1328
Bsa29I	ATCGAT	1 cut(s)	579
BsaBI	GATNNNNATC	1 cut(s)	585
BsaI	GGTCTC	3 cut(s)	202, 1088, 1629
BsaJI	CCNNGG	6 cut(s)	235, 330, 336, 357, 363, 1105
BsaWI	WCCGGW	1 cut(s)	271
BsaXI	ACNNNNNCTCC	2 cut(s)	383, 413
Bsc4I	CCNNNNNNNGG	6 cut(s)	209, 364, 740, 926, 1104, 1385
Bse118I	RCCGGY	2 cut(s)	138, 185
Bse1I	ACTGG	7 cut(s)	20, 220, 315, 343, 1154, 1386, 1404
Bse3DI	GCAATG	1 cut(s)	1611
Bse8I	GATNNNNATC	1 cut(s)	585
BseBI	CCWGG	2 cut(s)	359, 1009
BseCI	ATCGAT	1 cut(s)	579
BseDI	CCNNGG	6 cut(s)	235, 330, 336, 357, 363, 1105
BseGI	GGATG	8 cut(s)	84, 777, 921, 1083, 1149, 1171, 1446, 1506
BseJI	GATNNNNATC	1 cut(s)	585
BseLI	CCNNNNNNNGG	6 cut(s)	209, 364, 740, 926, 1104, 1385
BseMI	GCAATG	1 cut(s)	1611
BseMII	CTCAG	4 cut(s)	1062, 1083, 1287, 1354
BseNI	ACTGG	7 cut(s)	20, 220, 315, 343, 1154, 1386, 1404
BseRI	GAGGAG	1 cut(s)	587
BseSI	GKGCMC	1 cut(s)	297
BseXI	GCAGC	8 cut(s)	242, 262, 464, 937, 1256, 1335, 1345, 1387
BseYI	CCCAGC	2 cut(s)	524, 1139
BsgI	GTGCAG	1 cut(s)	1102
Bsh1236I	CGCG	2 cut(s)	105, 317
BshVI	ATCGAT	1 cut(s)	579
BsiHKAI	GWGCWC	2 cut(s)	231, 297
BsiHKCI	CYCGRG	1 cut(s)	644
BsiSI	CCGG	9 cut(s)	139, 186, 203, 259, 272, 369, 530, 1105, 1328
BslFI	GGGAC	1 cut(s)	687
BslI	CCNNNNNNNGG	6 cut(s)	209, 364, 740, 926, 1104, 1385
BsmAI	GTCTC	9 cut(s)	164, 202, 466, 714, 891, 1088, 1173, 1549, 1629
BsmBI	CGTCTC	3 cut(s)	164, 466, 891
BsmFI	GGGAC	1 cut(s)	687
BsmI	GAATGC	1 cut(s)	1059
Bso31I	GGTCTC	3 cut(s)	202, 1088, 1629
BsoBI	CYCGRG	1 cut(s)	644
Bsp119I	TTCGAA	1 cut(s)	1518
Bsp1286I	GDGCHC	2 cut(s)	231, 297
Bsp19I	CCATGG	1 cut(s)	336
BspCNI	CTCAG	4 cut(s)	1061, 1084, 1286, 1353
BspDI	ATCGAT	1 cut(s)	579
BspFNI	CGCG	2 cut(s)	105, 317
BspLI	GGNNCC	4 cut(s)	588, 777, 969, 1006
BspPI	GGATC	8 cut(s)	274, 426, 581, 594, 962, 975, 999, 1012
BspQI	GCTCTTC	1 cut(s)	236
BspT104I	TTCGAA	1 cut(s)	1518
BspTNI	GGTCTC	3 cut(s)	202, 1088, 1629
BsrBI	CCGCTC	1 cut(s)	155
BsrDI	GCAATG	1 cut(s)	1611
BsrFI	RCCGGY	2 cut(s)	138, 185
BsrI	ACTGG	7 cut(s)	20, 220, 315, 343, 1154, 1386, 1404
BssAI	RCCGGY	2 cut(s)	138, 185
BssECI	CCNNGG	6 cut(s)	235, 330, 336, 357, 363, 1105
BssSI	CACGAG	1 cut(s)	840
BssT1I	CCWWGG	2 cut(s)	330, 336
Bst2BI	CACGAG	1 cut(s)	840
Bst2UI	CCWGG	2 cut(s)	359, 1009
Bst4CI	ACNGT	1 cut(s)	1347
Bst6I	CTCTTC	3 cut(s)	236, 558, 1293
BstBI	TTCGAA	1 cut(s)	1518
BstC8I	GCNNGC	7 cut(s)	140, 144, 187, 780, 882, 1192, 1237
BstDEI	CTNAG	5 cut(s)	1048, 1092, 1164, 1273, 1340
BstDSI	CCRYGG	3 cut(s)	235, 336, 363
BstF5I	GGATG	8 cut(s)	84, 777, 921, 1083, 1149, 1171, 1446, 1506
BstFNI	CGCG	2 cut(s)	105, 317
BstH2I	RGCGCY	2 cut(s)	256, 525
BstHHI	GCGC	4 cut(s)	107, 255, 317, 524
BstMAI	GTCTC	9 cut(s)	164, 202, 466, 714, 891, 1088, 1173, 1549, 1629
BstMWI	GCNNNNNNNGC	7 cut(s)	54, 113, 152, 503, 1063, 1332, 1583
BstNI	CCWGG	2 cut(s)	359, 1009
BstNSI	RCATGY	3 cut(s)	900, 1243, 1516
BstPAI	GACNNNNGTC	1 cut(s)	1561
BstSCI	CCNGG	4 cut(s)	357, 1007, 1104, 1326
BstSLI	GKGCMC	1 cut(s)	297
BstUI	CGCG	2 cut(s)	105, 317
BstV1I	GCAGC	8 cut(s)	242, 262, 464, 937, 1256, 1335, 1345, 1387
BstV2I	GAAGAC	1 cut(s)	168
BstX2I	RGATCY	4 cut(s)	266, 586, 967, 1004
BstXI	CCANNNNNNTGG	1 cut(s)	337
BstYI	RGATCY	4 cut(s)	266, 586, 967, 1004
Bsu15I	ATCGAT	1 cut(s)	579
BsuI	GTATCC	1 cut(s)	1214
BsuTUI	ATCGAT	1 cut(s)	579
BtgI	CCRYGG	3 cut(s)	235, 336, 363
BtrI	CACGTC	1 cut(s)	1619
BtsCI	GGATG	8 cut(s)	84, 777, 921, 1083, 1149, 1171, 1446, 1506
BtsI	GCAGTG	1 cut(s)	284
BtsIMutI	CAGTG	6 cut(s)	284, 308, 521, 866, 1147, 1379
Cac8I	GCNNGC	7 cut(s)	140, 144, 187, 780, 882, 1192, 1237
CfoI	GCGC	4 cut(s)	107, 255, 317, 524
Cfr10I	RCCGGY	2 cut(s)	138, 185
Cfr13I	GGNCC	7 cut(s)	327, 340, 355, 495, 527, 776, 938
ClaI	ATCGAT	1 cut(s)	579
CseI	GACGC	3 cut(s)	483, 873, 1610
Csp6I	GTAC	8 cut(s)	223, 566, 634, 1026, 1045, 1110, 1122, 1252
CviAII	CATG	8 cut(s)	337, 388, 427, 825, 897, 1067, 1240, 1513
CviQI	GTAC	8 cut(s)	223, 566, 634, 1026, 1045, 1110, 1122, 1252
DdeI	CTNAG	5 cut(s)	1048, 1092, 1164, 1273, 1340
EaeI	YGGCCR	2 cut(s)	360, 366
Eam1104I	CTCTTC	3 cut(s)	236, 558, 1293
EarI	CTCTTC	3 cut(s)	236, 558, 1293
EciI	GGCGGA	1 cut(s)	25
Ecl136II	GAGCTC	1 cut(s)	229
Eco130I	CCWWGG	2 cut(s)	330, 336
Eco147I	AGGCCT	1 cut(s)	1335
Eco24I	GRGCYC	1 cut(s)	231
Eco31I	GGTCTC	3 cut(s)	202, 1088, 1629
Eco47I	GGWCC	1 cut(s)	340
Eco47III	AGCGCT	1 cut(s)	254
Eco53kI	GAGCTC	1 cut(s)	229
Eco57I	CTGAAG	2 cut(s)	700, 973
Eco88I	CYCGRG	1 cut(s)	644
EcoICRI	GAGCTC	1 cut(s)	229
EcoO109I	RGGNCCY	3 cut(s)	355, 776, 938
EcoRI	GAATTC	1 cut(s)	759
EcoRII	CCWGG	2 cut(s)	357, 1007
EcoT14I	CCWWGG	2 cut(s)	330, 336
EcoT22I	ATGCAT	1 cut(s)	898
EcoT38I	GRGCYC	1 cut(s)	231
ErhI	CCWWGG	2 cut(s)	330, 336
Esp3I	CGTCTC	3 cut(s)	164, 466, 891
FaeI	CATG	8 cut(s)	340, 391, 430, 828, 900, 1070, 1243, 1516
FalI	AAGNNNNNCTT	4 cut(s)	784, 816, 1241, 1273
FaqI	GGGAC	1 cut(s)	687
FatI	CATG	8 cut(s)	336, 387, 426, 824, 896, 1066, 1239, 1512
FauI	CCCGC	3 cut(s)	62, 110, 663
FauNDI	CATATG	2 cut(s)	694, 1459
FokI	GGATG	8 cut(s)	71, 784, 928, 1090, 1156, 1158, 1453, 1513
FriOI	GRGCYC	1 cut(s)	231
FspBI	CTAG	5 cut(s)	1119, 1362, 1466, 1592, 1678
GlaI	GCGC	4 cut(s)	106, 254, 316, 523
GsaI	CCCAGC	2 cut(s)	528, 1143
GsuI	CTGGAG	1 cut(s)	203
HaeII	RGCGCY	2 cut(s)	256, 525
HapII	CCGG	9 cut(s)	139, 186, 203, 259, 272, 369, 530, 1105, 1328
HgaI	GACGC	3 cut(s)	483, 873, 1610
HhaI	GCGC	4 cut(s)	107, 255, 317, 524
Hin1II	CATG	8 cut(s)	340, 391, 430, 828, 900, 1070, 1243, 1516
Hin6I	GCGC	4 cut(s)	105, 253, 315, 522
HinP1I	GCGC	4 cut(s)	105, 253, 315, 522
HincII	GTYRAC	2 cut(s)	1185, 1600
HindII	GTYRAC	2 cut(s)	1185, 1600
HinfI	GANTC	4 cut(s)	240, 536, 547, 1418
HpaI	GTTAAC	1 cut(s)	1185
HpaII	CCGG	9 cut(s)	139, 186, 203, 259, 272, 369, 530, 1105, 1328
HphI	GGTGA	6 cut(s)	101, 116, 672, 797, 1082, 1400
Hpy166II	GTNNAC	4 cut(s)	295, 680, 1185, 1600
Hpy188III	TCNNGA	8 cut(s)	590, 842, 971, 1037, 1050, 1295, 1466, 1648
Hpy8I	GTNNAC	4 cut(s)	295, 680, 1185, 1600
Hpy99I	CGWCG	2 cut(s)	477, 1623
HpyCH4III	ACNGT	1 cut(s)	1347
HpyCH4IV	ACGT	3 cut(s)	676, 1124, 1618
HpyF10VI	GCNNNNNNNGC	7 cut(s)	54, 113, 152, 503, 1063, 1332, 1583
HpyF3I	CTNAG	5 cut(s)	1048, 1092, 1164, 1273, 1340
HpySE526I	ACGT	3 cut(s)	676, 1124, 1618
Hsp92II	CATG	8 cut(s)	340, 391, 430, 828, 900, 1070, 1243, 1516
HspAI	GCGC	4 cut(s)	105, 253, 315, 522
KroI	GCCGGC	2 cut(s)	138, 185
KroNI	GCCGGC	2 cut(s)	140, 187
KspAI	GTTAAC	1 cut(s)	1185
LguI	GCTCTTC	1 cut(s)	236
Lsp1109I	GCAGC	8 cut(s)	242, 262, 464, 937, 1256, 1335, 1345, 1387
LweI	GCATC	5 cut(s)	93, 172, 906, 1134, 1558
MaeI	CTAG	5 cut(s)	1119, 1362, 1466, 1592, 1678
MaeII	ACGT	3 cut(s)	676, 1124, 1618
MaeIII	GTNAC	3 cut(s)	280, 306, 1645
MbiI	CCGCTC	1 cut(s)	155
MboII	GAAGA	8 cut(s)	168, 223, 545, 630, 633, 979, 1310, 1318
MfeI	CAATTG	2 cut(s)	436, 1230
MflI	RGATCY	4 cut(s)	266, 586, 967, 1004
MhlI	GDGCHC	2 cut(s)	231, 297
MluCI	AATT	9 cut(s)	436, 759, 788, 909, 955, 1230, 1520, 1528, 1652
MlyI	GAGTC	1 cut(s)	530
MmeI	TCCRAC	1 cut(s)	50
Mph1103I	ATGCAT	1 cut(s)	898
MroNI	GCCGGC	2 cut(s)	138, 185
MroXI	GAANNNNTTC	3 cut(s)	1335, 1514, 1663
MseI	TTAA	5 cut(s)	78, 351, 791, 959, 1184
MslI	CAYNNNNRTG	1 cut(s)	392
MspA1I	CMGCKG	2 cut(s)	1190, 1358
MspI	CCGG	9 cut(s)	139, 186, 203, 259, 272, 369, 530, 1105, 1328
MspR9I	CCNGG	4 cut(s)	359, 1009, 1106, 1328
MunI	CAATTG	2 cut(s)	436, 1230
Mva1269I	GAATGC	1 cut(s)	1059
MvaI	CCWGG	2 cut(s)	359, 1009
MvnI	CGCG	2 cut(s)	105, 317
MwoI	GCNNNNNNNGC	7 cut(s)	54, 113, 152, 503, 1063, 1332, 1583
NaeI	GCCGGC	2 cut(s)	140, 187
NciI	CCSGG	2 cut(s)	1106, 1328
NcoI	CCATGG	1 cut(s)	336
NdeI	CATATG	2 cut(s)	694, 1459
NgoMIV	GCCGGC	2 cut(s)	138, 185
NlaIII	CATG	8 cut(s)	340, 391, 430, 828, 900, 1070, 1243, 1516
NlaIV	GGNNCC	4 cut(s)	588, 777, 969, 1006
NmuCI	GTSAC	3 cut(s)	280, 306, 1645
NsiI	ATGCAT	1 cut(s)	898
NspI	RCATGY	3 cut(s)	900, 1243, 1516
NspV	TTCGAA	1 cut(s)	1518
PceI	AGGCCT	1 cut(s)	1335
PciI	ACATGT	1 cut(s)	1512
PciSI	GCTCTTC	1 cut(s)	236
PctI	GAATGC	1 cut(s)	1059
PdiI	GCCGGC	2 cut(s)	140, 187
PdmI	GAANNNNTTC	3 cut(s)	1335, 1514, 1663
PfeI	GAWTC	3 cut(s)	240, 547, 1418
PfoI	TCCNGGA	1 cut(s)	1007
PleI	GAGTC	1 cut(s)	530
PpsI	GAGTC	1 cut(s)	530
PscI	ACATGT	1 cut(s)	1512
PshAI	GACNNNNGTC	1 cut(s)	1561
Psp124BI	GAGCTC	1 cut(s)	231
Psp6I	CCWGG	2 cut(s)	357, 1007
PspFI	CCCAGC	2 cut(s)	524, 1139
PspGI	CCWGG	2 cut(s)	357, 1007
PspN4I	GGNNCC	4 cut(s)	588, 777, 969, 1006
PspPI	GGNCC	7 cut(s)	327, 340, 355, 495, 527, 776, 938
PsuI	RGATCY	4 cut(s)	266, 586, 967, 1004
PvuII	CAGCTG	2 cut(s)	1190, 1358
RsaI	GTAC	8 cut(s)	224, 567, 635, 1027, 1046, 1111, 1123, 1253
RsaNI	GTAC	8 cut(s)	223, 566, 634, 1026, 1045, 1110, 1122, 1252
RseI	CAYNNNNRTG	1 cut(s)	392
SacI	GAGCTC	1 cut(s)	231
SapI	GCTCTTC	1 cut(s)	236
SaqAI	TTAA	5 cut(s)	78, 351, 791, 959, 1184
Sau96I	GGNCC	7 cut(s)	327, 340, 355, 495, 527, 776, 938
SchI	GAGTC	1 cut(s)	530
ScrFI	CCNGG	4 cut(s)	359, 1009, 1106, 1328
SduI	GDGCHC	2 cut(s)	231, 297
SfaNI	GCATC	5 cut(s)	93, 172, 906, 1134, 1558
SfuI	TTCGAA	1 cut(s)	1518
SinI	GGWCC	1 cut(s)	340
SmiMI	CAYNNNNRTG	1 cut(s)	392
SmlI	CTYRAG	1 cut(s)	569
SmoI	CTYRAG	1 cut(s)	569
Sse9I	AATT	9 cut(s)	436, 759, 788, 909, 955, 1230, 1520, 1528, 1652
SseBI	AGGCCT	1 cut(s)	1335
SspMI	CTAG	5 cut(s)	1119, 1362, 1466, 1592, 1678
SstI	GAGCTC	1 cut(s)	231
StuI	AGGCCT	1 cut(s)	1335
StyD4I	CCNGG	4 cut(s)	357, 1007, 1104, 1326
StyI	CCWWGG	2 cut(s)	330, 336
TaaI	ACNGT	1 cut(s)	1347
TaiI	ACGT	3 cut(s)	679, 1127, 1621
TaqI	TCGA	6 cut(s)	421, 472, 579, 630, 1518, 1582
TaqII	GACCGA	2 cut(s)	184, 1611
TasI	AATT	9 cut(s)	436, 759, 788, 909, 955, 1230, 1520, 1528, 1652
TauI	GCSGC	4 cut(s)	51, 192, 250, 522
TfiI	GAWTC	3 cut(s)	240, 547, 1418
Tru1I	TTAA	5 cut(s)	78, 351, 791, 959, 1184
Tru9I	TTAA	5 cut(s)	78, 351, 791, 959, 1184
TscAI	CASTG	6 cut(s)	291, 315, 521, 866, 1154, 1386
TseFI	GTSAC	3 cut(s)	280, 306, 1645
TseI	GCWGC	8 cut(s)	250, 255, 477, 950, 1269, 1323, 1358, 1400
Tsp45I	GTSAC	3 cut(s)	280, 306, 1645
TspDTI	ATGAA	5 cut(s)	500, 1325, 1431, 1514, 1526
TspGWI	ACGGA	3 cut(s)	252, 289, 1164
TspRI	CASTG	6 cut(s)	291, 315, 521, 866, 1154, 1386
VneI	GTGCAC	1 cut(s)	293
VpaK11BI	GGWCC	1 cut(s)	340
XapI	RAATTY	3 cut(s)	759, 1520, 1652
XbaI	TCTAGA	1 cut(s)	1465
XceI	RCATGY	3 cut(s)	900, 1243, 1516
XmnI	GAANNNNTTC	3 cut(s)	1335, 1514, 1663
XspI	CTAG	5 cut(s)	1119, 1362, 1466, 1592, 1678
Zsp2I	ATGCAT	1 cut(s)	898

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.