MD06G1211400.v1.1

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0024257
Physical Location & Seq
Reverse (-)
34408574 .. 34412775
4202 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1211400.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGTGTGGAACAACTACTCCGAATGTTTCGGATATTGTGAAACATAGGACCCTGATCATTGGATCAACAGGATTCATTGGCCGGTTCGTAGCTGAAGCCAGCCTTCACTCAGGTCAGCCAACCTATCTCCTTGTCCGCCCCGGCTCAGTCGTGGGCTCTTCCAAGAGCACCACCACTATCGGGTACTTGCAAGAGAGAGGAGCTATAGTCATAACGGGCTCCATTGATGATCAAGATTCAATGGAGAAAGTGTTGAAGGAGCACAAGATTGAAGTTGTCGTATCAGTCGTGGGTGGTGCAAGAATTTTGGATCAGCTTATTTTACTGGAAGCCATCAAAGCTGTGGGCACTGTTAAGAGATTTTTGCCATCTGAGTTTGGGCATGACATAGACAGAGCTGATCCAGTAGAGCCAGGACTAACTATGTACAATGAGAAGAGAAAAGTCAGAAGGGCCACTGAGGCTGCTGGAATTCCCTACACTTACATATGCTGCAACTCCATTGCTGGTTGGCCTTACCATGACAACATTCACCCCGCCGATGTCCTCCCGCCGTTGGATCGATTCCACATCTATGGTGATGGCACTGTCAAAGCTTACTTTGTGACTGGTTCTGACATCGGAAAGTTCACCATGAAAACAATCGGTGACGTCCGAACAGTCAACAAATCTGTTCATTTTCGACCACAATGCAACCTGTTGAACATCAACCAGCTTGCATCTTTGTGGGAGAAGAGCATTGGACGCACACTCCCTAGACTCACTGTTACAGAAGACGACCTTCTTTCTGCTGCCAAAGAAATGATCATCCCACAAAGCATTGTGGCATCCTTCACTCATGACATTTTCATCAAGGGCTGCCAAGTAAACTACGACATAGAAAACCCGAACGACATTGAAGTCTGTTCTCTCTATCCCGACACACCATTTCAGACCGTGGAGGAGTGCTTCAAAGATTTTGCCACAAGGGTTGTCGTCGTTAATGTTCCAAAAGTAGCTGAAAAATCAAAGAGCAACGATTTCATCGTCGTGCCGAATGCTAAACCAGAAGCGTTAGCTGTTGCACCAGCATCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01110 map00941 map01110
Pfam Domains
Protein Families
NmrA

Protein Analysis

360

Amino Acids

39.3

Weight (kDa)

6.16

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 15 - 315 1.2e-49 NmrA-like family
Epimerase PF01370 18 - 98 3e-06 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 21 - 167 3.7e-06 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024257)

Species Orthologous Gene IDs
malus_domestica MD06G1211400.v1.1
prunus_persica Prupe.5G217500_v2.0.a1
pyrus_communis pycom06g18890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 899
AatII GACGTC 1 cut(s) 654
AciI CCGC 3 cut(s) 136, 537, 551
AclWI GGATC 4 cut(s) 70, 318, 395, 567
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 2 cut(s) 303, 471
AcuI CTGAAG 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 651
AfaI GTAC 2 cut(s) 185, 428
AfiI CCNNNNNNNGG 2 cut(s) 180, 556
AgsI TTSAA 6 cut(s) 240, 256, 272, 703, 899, 952
AjnI CCWGG 1 cut(s) 412
AloI GAACNNNNNNTCC 1 cut(s) 33
Alw21I GWGCWC 2 cut(s) 170, 264
AlwI GGATC 4 cut(s) 70, 318, 395, 567
AoxI GGCC 3 cut(s) 79, 453, 512
ApeKI GCWGC 4 cut(s) 464, 492, 791, 858
ApoI RAATTY 2 cut(s) 303, 471
AspS9I GGNCC 2 cut(s) 48, 453
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 4 cut(s) 524, 590, 622, 659
AvaII GGWCC 1 cut(s) 48
BaeGI GKGCMC 1 cut(s) 350
BanII GRGCYC 2 cut(s) 158, 221
BbsI GAAGAC 1 cut(s) 780
Bbv12I GWGCWC 2 cut(s) 170, 264
BbvI GCAGC 4 cut(s) 451, 479, 778, 845
BccI CCATC 3 cut(s) 341, 376, 575
BceAI ACGGC 1 cut(s) 538
BcgI CGANNNNNNTGC 2 cut(s) 672, 706
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 3 cut(s) 54, 229, 804
BcnI CCSGG 1 cut(s) 141
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 1 cut(s) 204
BglI GCCNNNNNGGC 1 cut(s) 461
BisI GCNGC 4 cut(s) 465, 493, 792, 859
BlsI GCNGC 4 cut(s) 466, 494, 793, 860
Bme1390I CCNGG 2 cut(s) 141, 414
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 2 cut(s) 48, 453
BmiI GGNNCC 2 cut(s) 50, 220
BmrFI CCNGG 2 cut(s) 141, 414
BmsI GCATC 2 cut(s) 728, 836
BpiI GAAGAC 1 cut(s) 780
BpuMI CCSGG 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 562
BsaHI GRCGYC 1 cut(s) 651
BsaJI CCNNGG 2 cut(s) 139, 936
BsaXI ACNNNNNCTCC 7 cut(s) 31, 192, 222, 236, 266, 735, 765
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 556
Bse118I RCCGGY 1 cut(s) 81
Bse1I ACTGG 3 cut(s) 330, 404, 613
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 1 cut(s) 414
BseCI ATCGAT 1 cut(s) 562
BseDI CCNNGG 2 cut(s) 139, 936
BseGI GGATG 2 cut(s) 807, 827
BseLI CCNNNNNNNGG 2 cut(s) 180, 556
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 4 cut(s) 123, 159, 363, 450
BseNI ACTGG 3 cut(s) 330, 404, 613
BseRI GAGGAG 2 cut(s) 213, 956
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 4 cut(s) 451, 479, 778, 845
BshFI GGCC 3 cut(s) 81, 455, 514
BshVI ATCGAT 1 cut(s) 562
BsiHKAI GWGCWC 2 cut(s) 170, 264
BsiSI CCGG 2 cut(s) 82, 141
BslI CCNNNNNNNGG 2 cut(s) 180, 556
BsmI GAATGC 1 cut(s) 1042
BsnI GGCC 3 cut(s) 81, 455, 514
Bsp1286I GDGCHC 5 cut(s) 158, 170, 221, 264, 350
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 7 cut(s) 54, 62, 229, 310, 400, 559, 804
BspACI CCGC 3 cut(s) 136, 537, 551
BspANI GGCC 3 cut(s) 81, 455, 514
BspCNI CTCAG 4 cut(s) 122, 158, 364, 451
BspDI ATCGAT 1 cut(s) 562
BspHI TCATGA 1 cut(s) 838
BspLI GGNNCC 2 cut(s) 50, 220
BspPI GGATC 4 cut(s) 70, 318, 395, 567
BspQI GCTCTTC 2 cut(s) 163, 728
BsrDI GCAATG 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 81
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 3 cut(s) 330, 404, 613
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 2 cut(s) 139, 936
BssMI GATC 7 cut(s) 54, 62, 229, 310, 400, 559, 804
BssNI GRCGYC 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 5 cut(s) 352, 589, 661, 766, 937
Bst6I CTCTTC 3 cut(s) 163, 431, 728
BstACI GRCGYC 1 cut(s) 651
BstAUI TGTACA 1 cut(s) 426
BstC8I GCNNGC 2 cut(s) 100, 717
BstDEI CTNAG 4 cut(s) 109, 145, 372, 459
BstDSI CCRYGG 1 cut(s) 936
BstF5I GGATG 2 cut(s) 807, 827
BstKTI GATC 7 cut(s) 57, 65, 232, 313, 403, 562, 807
BstMBI GATC 7 cut(s) 54, 62, 229, 310, 400, 559, 804
BstMWI GCNNNNNNNGC 3 cut(s) 338, 461, 744
BstNI CCWGG 1 cut(s) 414
BstSCI CCNGG 2 cut(s) 139, 412
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 350
BstV1I GCAGC 4 cut(s) 451, 479, 778, 845
BstV2I GAAGAC 1 cut(s) 780
BstXI CCANNNNNNTGG 1 cut(s) 575
Bsu15I ATCGAT 1 cut(s) 562
BsuRI GGCC 3 cut(s) 81, 455, 514
BsuTUI ATCGAT 1 cut(s) 562
BtgI CCRYGG 1 cut(s) 936
BtsCI GGATG 2 cut(s) 807, 827
BtsIMutI CAGTG 4 cut(s) 348, 456, 585, 762
Cac8I GCNNGC 2 cut(s) 100, 717
CciI TCATGA 1 cut(s) 838
Cfr10I RCCGGY 1 cut(s) 81
Cfr13I GGNCC 2 cut(s) 48, 453
ClaI ATCGAT 1 cut(s) 562
CseI GACGC 1 cut(s) 753
Csp6I GTAC 2 cut(s) 184, 427
CspCI CAANNNNNGTGG 2 cut(s) 952, 987
CviAII CATG 4 cut(s) 383, 521, 634, 839
CviQI GTAC 2 cut(s) 184, 427
DdeI CTNAG 4 cut(s) 109, 145, 372, 459
DpnI GATC 7 cut(s) 56, 64, 231, 312, 402, 561, 806
DpnII GATC 7 cut(s) 54, 62, 229, 310, 400, 559, 804
DrdI GACNNNNNNGTC 1 cut(s) 899
DseDI GACNNNNNNGTC 1 cut(s) 899
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 3 cut(s) 163, 431, 728
EarI CTCTTC 3 cut(s) 163, 431, 728
EciI GGCGGA 1 cut(s) 125
Eco24I GRGCYC 2 cut(s) 158, 221
Eco47I GGWCC 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 114
EcoO109I RGGNCCY 1 cut(s) 48
EcoRI GAATTC 1 cut(s) 471
EcoRII CCWGG 1 cut(s) 412
EcoT38I GRGCYC 2 cut(s) 158, 221
FaeI CATG 4 cut(s) 386, 524, 637, 842
FalI AAGNNNNNCTT 4 cut(s) 87, 119, 765, 797
FatI CATG 4 cut(s) 382, 520, 633, 838
FauI CCCGC 2 cut(s) 544, 558
FauNDI CATATG 1 cut(s) 488
FbaI TGATCA 3 cut(s) 54, 229, 804
Fnu4HI GCNGC 4 cut(s) 465, 493, 792, 859
FokI GGATG 2 cut(s) 794, 814
FriOI GRGCYC 2 cut(s) 158, 221
Fsp4HI GCNGC 4 cut(s) 465, 493, 792, 859
FspBI CTAG 1 cut(s) 756
GluI GCNGC 4 cut(s) 465, 493, 792, 859
HaeIII GGCC 3 cut(s) 81, 455, 514
HapII CCGG 2 cut(s) 82, 141
HgaI GACGC 1 cut(s) 753
Hin1I GRCGYC 1 cut(s) 651
Hin1II CATG 4 cut(s) 386, 524, 637, 842
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 1 cut(s) 594
HinfI GANTC 4 cut(s) 72, 236, 564, 759
HpaII CCGG 2 cut(s) 82, 141
HphI GGTGA 4 cut(s) 524, 590, 622, 659
Hpy166II GTNNAC 3 cut(s) 630, 664, 868
Hpy188I TCNGA 8 cut(s) 21, 31, 373, 449, 616, 623, 656, 933
Hpy188III TCNNGA 3 cut(s) 233, 839, 917
Hpy8I GTNNAC 3 cut(s) 630, 664, 868
Hpy99I CGWCG 2 cut(s) 980, 1031
HpyAV CCTTC 5 cut(s) 113, 250, 444, 791, 841
HpyCH4III ACNGT 5 cut(s) 352, 589, 661, 766, 937
HpyCH4IV ACGT 1 cut(s) 651
HpyCH4V TGCA 6 cut(s) 190, 299, 495, 693, 719, 1064
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 461, 744
HpyF3I CTNAG 4 cut(s) 109, 145, 372, 459
HpySE526I ACGT 1 cut(s) 651
Hsp92I GRCGYC 1 cut(s) 651
Hsp92II CATG 4 cut(s) 386, 524, 637, 842
Ksp22I TGATCA 3 cut(s) 54, 229, 804
Kzo9I GATC 7 cut(s) 54, 62, 229, 310, 400, 559, 804
LguI GCTCTTC 2 cut(s) 163, 728
LmnI GCTCC 3 cut(s) 200, 224, 259
Lsp1109I GCAGC 4 cut(s) 451, 479, 778, 845
LweI GCATC 2 cut(s) 728, 836
MaeI CTAG 1 cut(s) 756
MaeII ACGT 1 cut(s) 651
MaeIII GTNAC 3 cut(s) 604, 647, 766
MalI GATC 7 cut(s) 56, 64, 231, 312, 402, 561, 806
MboI GATC 7 cut(s) 54, 62, 229, 310, 400, 559, 804
MboII GAAGA 4 cut(s) 150, 448, 745, 785
MhlI GDGCHC 5 cut(s) 158, 170, 221, 264, 350
MluCI AATT 2 cut(s) 303, 471
MlyI GAGTC 1 cut(s) 753
MmeI TCCRAC 1 cut(s) 537
MnlI CCTC 4 cut(s) 191, 454, 557, 934
MseI TTAA 2 cut(s) 354, 981
MslI CAYNNNNRTG 3 cut(s) 573, 724, 1028
MspI CCGG 2 cut(s) 82, 141
MspR9I CCNGG 2 cut(s) 141, 414
Mva1269I GAATGC 1 cut(s) 1042
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 3 cut(s) 338, 461, 744
NciI CCSGG 1 cut(s) 141
NdeI CATATG 1 cut(s) 488
NdeII GATC 7 cut(s) 54, 62, 229, 310, 400, 559, 804
NlaIII CATG 4 cut(s) 386, 524, 637, 842
NlaIV GGNNCC 2 cut(s) 50, 220
NmuCI GTSAC 2 cut(s) 604, 647
PagI TCATGA 1 cut(s) 838
PciSI GCTCTTC 2 cut(s) 163, 728
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 1023
PctI GAATGC 1 cut(s) 1042
PfeI GAWTC 3 cut(s) 72, 236, 564
PkrI GCNGC 4 cut(s) 466, 494, 793, 860
PleI GAGTC 1 cut(s) 753
PpsI GAGTC 1 cut(s) 753
PpuMI RGGWCCY 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 48
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 50, 220
PspPI GGNCC 2 cut(s) 48, 453
PspPPI RGGWCCY 1 cut(s) 48
RsaI GTAC 2 cut(s) 185, 428
RsaNI GTAC 2 cut(s) 184, 427
RseI CAYNNNNRTG 3 cut(s) 573, 724, 1028
SapI GCTCTTC 2 cut(s) 163, 728
SaqAI TTAA 2 cut(s) 354, 981
SatI GCNGC 4 cut(s) 465, 493, 792, 859
Sau3AI GATC 7 cut(s) 54, 62, 229, 310, 400, 559, 804
Sau96I GGNCC 2 cut(s) 48, 453
SchI GAGTC 1 cut(s) 753
ScrFI CCNGG 2 cut(s) 141, 414
SduI GDGCHC 5 cut(s) 158, 170, 221, 264, 350
SfaNI GCATC 2 cut(s) 728, 836
SfcI CTRYAG 1 cut(s) 204
SinI GGWCC 1 cut(s) 48
SmiMI CAYNNNNRTG 3 cut(s) 573, 724, 1028
Sse9I AATT 2 cut(s) 303, 471
SsiI CCGC 3 cut(s) 136, 537, 551
SspMI CTAG 1 cut(s) 756
StyD4I CCNGG 2 cut(s) 139, 412
TaaI ACNGT 5 cut(s) 352, 589, 661, 766, 937
TaiI ACGT 1 cut(s) 654
TaqI TCGA 2 cut(s) 562, 682
TasI AATT 2 cut(s) 303, 471
TatI WGTACW 1 cut(s) 426
TfiI GAWTC 3 cut(s) 72, 236, 564
Tru1I TTAA 2 cut(s) 354, 981
Tru9I TTAA 2 cut(s) 354, 981
TscAI CASTG 4 cut(s) 355, 463, 592, 769
TseFI GTSAC 2 cut(s) 604, 647
TseI GCWGC 4 cut(s) 464, 492, 791, 858
Tsp45I GTSAC 2 cut(s) 604, 647
TspDTI ATGAA 5 cut(s) 64, 650, 665, 838, 1012
TspRI CASTG 4 cut(s) 355, 463, 592, 769
VpaK11BI GGWCC 1 cut(s) 48
XapI RAATTY 2 cut(s) 303, 471
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XspI CTAG 1 cut(s) 756
ZraI GACGTC 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.