MD06G1215000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0028312
Physical Location & Seq
Reverse (-)
34744589 .. 34745342
754 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1215000.v1.1.491

Sequence Viewer

Length: 399 bp
ATGGTTTATTATGGCTTATCATTTCCTGTGCTGATAGATCAACAAAGCAAGGTCATGTCTCCAACTTCATGGGTTAAGAAGTTAGTTCCAATGAAAGGCCACAAGAAGGCCAGTGAAAGCCGGCTACTTCCTCTAACCGGCTCTTCTGCTAGAACCAGCCTAGAGATGCAGGAAGTGGTTATAGGTGCTGATTTTCGATGCAGCAAGTGTCAAAAGAGCGCATGCCAGGTGATATCGGATGTTGATGATGTGGATTCCATGGTGGTACATCTTTTGGATAAGAAGGTTACACTCACTTGTGAACCAGTCCGTAAAGGATCTCTAAGAAAAATCTCGGCCATCTGCGGAAAACTATCTCACAGAGCCTTAAGGACTGCAAGGTTTTATACGCGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

133

Amino Acids

14.74

Weight (kDa)

9.74

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028312)

Species Orthologous Gene IDs
malus_domestica MD06G1215000.v1.1
pyrus_communis pycom06g19160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 391
AciI CCGC 1 cut(s) 345
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 1 cut(s) 336
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 3 cut(s) 95, 106, 137
AflII CTTAAG 1 cut(s) 367
AflIII ACRYGT 1 cut(s) 389
AjnI CCWGG 1 cut(s) 225
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 1 cut(s) 325
AoxI GGCC 3 cut(s) 97, 108, 336
ApeKI GCWGC 1 cut(s) 201
AspLEI GCGC 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 241
BbvI GCAGC 1 cut(s) 213
BccI CCATC 1 cut(s) 347
BciT130I CCWGG 1 cut(s) 227
BcoDI GTCTC 1 cut(s) 63
BfaI CTAG 2 cut(s) 150, 161
BfrI CTTAAG 1 cut(s) 367
BisI GCNGC 1 cut(s) 202
BlsI GCNGC 1 cut(s) 203
Bme1390I CCNGG 1 cut(s) 227
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 2 cut(s) 156, 188
BsaJI CCNNGG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 106, 137
Bse118I RCCGGY 2 cut(s) 120, 137
Bse1I ACTGG 2 cut(s) 111, 305
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 3 cut(s) 95, 106, 137
BseNI ACTGG 2 cut(s) 111, 305
BseXI GCAGC 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 391
BshFI GGCC 3 cut(s) 99, 110, 338
BsiSI CCGG 2 cut(s) 121, 138
BslI CCNNNNNNNGG 3 cut(s) 95, 106, 137
BsmAI GTCTC 1 cut(s) 63
BsnI GGCC 3 cut(s) 99, 110, 338
Bsp143I GATC 2 cut(s) 37, 317
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 1 cut(s) 345
BspANI GGCC 3 cut(s) 99, 110, 338
BspFNI CGCG 1 cut(s) 391
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 148
BspTI CTTAAG 1 cut(s) 367
BsrFI RCCGGY 2 cut(s) 120, 137
BsrI ACTGG 2 cut(s) 111, 305
BssAI RCCGGY 2 cut(s) 120, 137
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 2 cut(s) 37, 317
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 227
Bst6I CTCTTC 1 cut(s) 148
BstAFI CTTAAG 1 cut(s) 367
BstC8I GCNNGC 2 cut(s) 122, 223
BstDEI CTNAG 1 cut(s) 323
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 391
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 2 cut(s) 40, 320
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 2 cut(s) 37, 317
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 225
BstSCI CCNGG 1 cut(s) 225
BstUI CGCG 1 cut(s) 391
BstV1I GCAGC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 317
BstXI CCANNNNNNTGG 1 cut(s) 69
BstYI RGATCY 1 cut(s) 317
BsuRI GGCC 3 cut(s) 99, 110, 338
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 1 cut(s) 244
BtsIMutI CAGTG 2 cut(s) 118, 394
Cac8I GCNNGC 2 cut(s) 122, 223
CfoI GCGC 1 cut(s) 221
Cfr10I RCCGGY 2 cut(s) 120, 137
CseI GACGC 1 cut(s) 380
Csp6I GTAC 1 cut(s) 266
CviAII CATG 4 cut(s) 55, 69, 222, 259
CviJI RGCY 9 cut(s) 15, 99, 110, 120, 124, 141, 159, 338, 365
CviKI_1 RGCY 9 cut(s) 15, 99, 110, 120, 124, 141, 159, 338, 365
CviQI GTAC 1 cut(s) 266
DdeI CTNAG 1 cut(s) 323
DpnI GATC 2 cut(s) 39, 319
DpnII GATC 2 cut(s) 37, 317
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 258
Eco32I GATATC 1 cut(s) 234
EcoRII CCWGG 1 cut(s) 225
EcoRV GATATC 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 4 cut(s) 58, 72, 225, 262
FaiI YATR 7 cut(s) 12, 56, 70, 182, 223, 260, 387
FatI CATG 4 cut(s) 54, 68, 221, 258
Fnu4HI GCNGC 1 cut(s) 202
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 1 cut(s) 202
FspBI CTAG 2 cut(s) 150, 161
GlaI GCGC 1 cut(s) 220
GluI GCNGC 1 cut(s) 202
HaeIII GGCC 3 cut(s) 99, 110, 338
HapII CCGG 2 cut(s) 121, 138
HgaI GACGC 1 cut(s) 380
HhaI GCGC 1 cut(s) 221
Hin1II CATG 4 cut(s) 58, 72, 225, 262
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HinfI GANTC 1 cut(s) 254
HpaII CCGG 2 cut(s) 121, 138
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 1 cut(s) 302
Hpy188I TCNGA 1 cut(s) 238
Hpy8I GTNNAC 1 cut(s) 302
HpyAV CCTTC 2 cut(s) 100, 277
HpyCH4V TGCA 3 cut(s) 169, 201, 377
HpyF3I CTNAG 1 cut(s) 323
Hsp92II CATG 4 cut(s) 58, 72, 225, 262
HspAI GCGC 1 cut(s) 219
KroI GCCGGC 1 cut(s) 120
KroNI GCCGGC 1 cut(s) 122
Kzo9I GATC 2 cut(s) 37, 317
LguI GCTCTTC 1 cut(s) 148
LpnPI CCDG 9 cut(s) 39, 124, 134, 151, 155, 169, 212, 239, 318
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 2 cut(s) 156, 188
MaeI CTAG 2 cut(s) 150, 161
MaeIII GTNAC 2 cut(s) 286, 392
MalI GATC 2 cut(s) 39, 319
MboI GATC 2 cut(s) 37, 317
MboII GAAGA 1 cut(s) 135
MflI RGATCY 1 cut(s) 317
MluI ACGCGT 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 86
MnlI CCTC 1 cut(s) 141
MroNI GCCGGC 1 cut(s) 120
MseI TTAA 2 cut(s) 75, 368
MspCI CTTAAG 1 cut(s) 367
MspI CCGG 2 cut(s) 121, 138
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 1 cut(s) 391
NaeI GCCGGC 1 cut(s) 122
NcoI CCATGG 1 cut(s) 258
NdeII GATC 2 cut(s) 37, 317
NgoMIV GCCGGC 1 cut(s) 120
NlaIII CATG 4 cut(s) 58, 72, 225, 262
NmeAIII GCCGAG 1 cut(s) 314
NmuCI GTSAC 1 cut(s) 392
NspI RCATGY 1 cut(s) 225
PaeI GCATGC 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 148
PdiI GCCGGC 1 cut(s) 122
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PsuI RGATCY 1 cut(s) 317
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
SapI GCTCTTC 1 cut(s) 148
SaqAI TTAA 2 cut(s) 75, 368
SatI GCNGC 1 cut(s) 202
Sau3AI GATC 2 cut(s) 37, 317
ScrFI CCNGG 1 cut(s) 227
SetI ASST 5 cut(s) 54, 187, 231, 288, 383
SfaNI GCATC 2 cut(s) 156, 188
SmlI CTYRAG 1 cut(s) 367
SmoI CTYRAG 1 cut(s) 367
SphI GCATGC 1 cut(s) 225
SsiI CCGC 1 cut(s) 345
SspMI CTAG 2 cut(s) 150, 161
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 258
TaqI TCGA 1 cut(s) 196
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 2 cut(s) 75, 368
Tru9I TTAA 2 cut(s) 75, 368
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 1 cut(s) 201
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 2 cut(s) 57, 107
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 1 cut(s) 118
Vha464I CTTAAG 1 cut(s) 367
XceI RCATGY 1 cut(s) 225
XspI CTAG 2 cut(s) 150, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.