Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0024260

Physical Location & Seq

Genomic Coordinates Forward (+)

35128074 .. 35129616

Gene Structure 1543 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1218400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGAAGAGAATAACTAGGTCACGGTTTGATCCGAATACTCGTGAAGAGGACAATAATCTGCAGGAAGATCAACTACTTGAGAATGGTGCAACTTCAAGCTCCGTTGAGCCTCCAAATTCAGCAGCAAAGCCTCCATCTGAGCAATCTCTGACGCCGAAAATTGCAAGTATTCTTGACAACAATGTGTACGAGGTGCCTATAAAGGCTATTCCAGAATCAGCGGAGTGGATATTTTTAGTGGTTGCCCTCGGCCTAGAGATGTTATCAGCTGCTTTCGACCAGGCTTCGTCCCCAAGTAAACCCCACTACGCGCTACTGAGCATGCTGTGTGCTGTTGCAGCTCTTCTTACTACCATCTGGGAGCTCACTTACAAGGCTATAAGAGAAAGAGTGGTATTAAGGAGGTCGGGATTGCTAATGCTGCCCTACTTTTATTATCCGCCTCCTCGGAACACAGTTTTCGGTGATCTCTATGACATTTATGGATTAGTTGGCGGGATCTCTCAGTCCGCTTGCTCTGCAGTTCAGTACGTTTACTTTCTCTGGCACGCTGATAATCCAATCAAATCATCCCTCTTGCCAGCCATCTTCCTTATGTGTTTGGGTGGTTCGAGACTAAATAGGAACCGGAGGTTCAGTAGTAATGTTGATAGCAACAATATTTTGTTGACGAGGGAGCTGACCGCCAACCGACCTTTGCTCGATGATTCTTCTACTTCTGCTTGGAGTCTCAGTGTCCAAACCGAACGAGAACCATCAAGCAGCTTACAATTATTTTTCGGTAAAAACCCCTCACCCACAGTTTTCACTGCGAGCAAGATTGCGGATGAAGAGAAAAATCCACTTCAAATCCTAGTTGTGGATGACCAAATGGTTCCAATAACAAATCTACCTTATCCAGTCAAAGTGGAAATTGTTGTTCTTGATGGTGACTTTCCAACTGGAGATGATGACAACTGGACAGAAGAAGAATTTGAGAACAACATCTTGAGACAGCTTACTGGGCGAAGGCGACACTTGCTTGCCGGAGAAGTAAGGCTCACGGTGAGAGATGGGTCAGCTACAATTGGAGATGTTGAGTTCACAGAGAGTTCGAGCTGGATTAGGAGCAAAAAATTTAGGCTGGGAGCACGAGTGATACCAAGCCCAGGCTATCAGGGTTTAAGGATCCGTGCAGCGGTCACGGAAGCTTTCGTTGTTAAAGATCATCGTGGCAAGCATCATCTTCCTCGCTACTTATCCCATACGAGAAACAATTCTGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002237 GO:0002239 GO:0002682 GO:0002831 GO:0002833 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009889 GO:0009987 GO:0010033 GO:0010112 GO:0010224 GO:0010337 GO:0010468 GO:0010556 GO:0010565 GO:0010817 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0031349 GO:0032101 GO:0032103 GO:0032350 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043900 GO:0043902 GO:0044424 GO:0044464 GO:0045088 GO:0046885 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0071216 GO:0071219 GO:0080090 GO:0080134 GO:0080142 GO:0097159 GO:0098542 GO:0140110 GO:1900424 GO:1900426 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Calmodulin_bind

Protein Analysis

422

Amino Acids

47.13

Weight (kDa)

6.22

Isoelectric Point (pI)

57.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calmodulin_bind	PF07887	256 - 403	2.4e-49	Calmodulin binding protein-like N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0024260)

Species	Orthologous Gene IDs
malus_domestica	MD06G1218400.v1.1
pyrus_communis	pycom06g19490 pycom06g19520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	193
AccII	CGCG	1 cut(s)	311
AciI	CCGC	7 cut(s)	221, 440, 495, 510, 684, 824, 1178
AclWI	GGATC	4 cut(s)	23, 506, 1162, 1175
AcsI	RAATTY	3 cut(s)	115, 971, 1115
AcyI	GRCGYC	1 cut(s)	152
AfaI	GTAC	2 cut(s)	188, 530
AfiI	CCNNNNNNNGG	3 cut(s)	859, 1148, 1177
AgsI	TTSAA	2 cut(s)	96, 848
AjnI	CCWGG	2 cut(s)	279, 1147
Alw21I	GWGCWC	2 cut(s)	366, 1132
Alw26I	GTCTC	3 cut(s)	607, 734, 985
AlwI	GGATC	4 cut(s)	23, 506, 1162, 1175
AoxI	GGCC	1 cut(s)	250
ApeKI	GCWGC	6 cut(s)	122, 269, 338, 421, 762, 1175
ApoI	RAATTY	3 cut(s)	115, 971, 1115
Asp700I	GAANNNNTTC	1 cut(s)	1255
AspLEI	GCGC	1 cut(s)	313
AsuHPI	GGTGA	4 cut(s)	476, 786, 941, 1057
BamHI	GGATCC	1 cut(s)	1167
BanI	GGYRCC	1 cut(s)	193
BanII	GRGCYC	1 cut(s)	366
BarI	GAAGNNNNNNTAC	2 cut(s)	57, 89
BauI	CACGAG	2 cut(s)	39, 1131
Bbv12I	GWGCWC	2 cut(s)	366, 1132
BbvI	GCAGC	6 cut(s)	134, 256, 350, 408, 774, 1187
BccI	CCATC	6 cut(s)	142, 362, 593, 763, 920, 1046
BciT130I	CCWGG	2 cut(s)	281, 1149
BcoDI	GTCTC	3 cut(s)	607, 734, 985
BfaI	CTAG	3 cut(s)	15, 254, 854
BfmI	CTRYAG	2 cut(s)	59, 519
BisI	GCNGC	6 cut(s)	123, 270, 339, 422, 763, 1176
BlsI	GCNGC	6 cut(s)	124, 271, 340, 423, 764, 1177
Bme1390I	CCNGG	2 cut(s)	281, 1149
BmiI	GGNNCC	4 cut(s)	195, 626, 876, 1169
BmrFI	CCNGG	2 cut(s)	281, 1149
BmrI	ACTGGG	1 cut(s)	1011
BmsI	GCATC	1 cut(s)	1228
BmuI	ACTGGG	1 cut(s)	1011
BpmI	CTGGAG	1 cut(s)	963
BpuEI	CTTGAG	2 cut(s)	98, 1009
BsaHI	GRCGYC	1 cut(s)	152
BsaJI	CCNNGG	3 cut(s)	247, 446, 1147
BsaWI	WCCGGW	1 cut(s)	627
BsaXI	ACNNNNNCTCC	6 cut(s)	622, 652, 718, 748, 1119, 1149
Bsc4I	CCNNNNNNNGG	3 cut(s)	859, 1148, 1177
Bse1I	ACTGG	4 cut(s)	899, 946, 962, 1006
BseBI	CCWGG	2 cut(s)	281, 1149
BseDI	CCNNGG	3 cut(s)	247, 446, 1147
BseGI	GGATG	3 cut(s)	569, 832, 868
BseLI	CCNNNNNNNGG	3 cut(s)	859, 1148, 1177
BseMII	CTCAG	4 cut(s)	129, 308, 518, 745
BseNI	ACTGG	4 cut(s)	899, 946, 962, 1006
BseRI	GAGGAG	1 cut(s)	435
BseXI	GCAGC	6 cut(s)	134, 256, 350, 408, 774, 1187
BseYI	CCCAGC	1 cut(s)	1123
BsgI	GTGCAG	1 cut(s)	1194
Bsh1236I	CGCG	1 cut(s)	311
BshFI	GGCC	1 cut(s)	252
BshNI	GGYRCC	1 cut(s)	193
BsiHKAI	GWGCWC	2 cut(s)	366, 1132
BsiSI	CCGG	2 cut(s)	628, 1026
BslFI	GGGAC	1 cut(s)	274
BslI	CCNNNNNNNGG	3 cut(s)	859, 1148, 1177
BsmAI	GTCTC	3 cut(s)	607, 734, 985
BsmFI	GGGAC	1 cut(s)	274
BsnI	GGCC	1 cut(s)	252
Bsp1286I	GDGCHC	2 cut(s)	366, 1132
Bsp143I	GATC	6 cut(s)	28, 67, 466, 498, 1167, 1204
BspACI	CCGC	7 cut(s)	221, 440, 495, 510, 684, 824, 1178
BspANI	GGCC	1 cut(s)	252
BspCNI	CTCAG	4 cut(s)	130, 309, 517, 744
BspFNI	CGCG	1 cut(s)	311
BspLI	GGNNCC	4 cut(s)	195, 626, 876, 1169
BspMAI	CTGCAG	2 cut(s)	63, 523
BspPI	GGATC	4 cut(s)	23, 506, 1162, 1175
BspQI	GCTCTTC	1 cut(s)	348
BspT107I	GGYRCC	1 cut(s)	193
BsrI	ACTGG	4 cut(s)	899, 946, 962, 1006
BssECI	CCNNGG	3 cut(s)	247, 446, 1147
BssMI	GATC	6 cut(s)	28, 67, 466, 498, 1167, 1204
BssNI	GRCGYC	1 cut(s)	152
BssSI	CACGAG	2 cut(s)	39, 1131
Bst2BI	CACGAG	2 cut(s)	39, 1131
Bst2UI	CCWGG	2 cut(s)	281, 1149
Bst4CI	ACNGT	4 cut(s)	24, 457, 802, 1045
Bst6I	CTCTTC	3 cut(s)	39, 348, 825
BstACI	GRCGYC	1 cut(s)	152
BstC8I	GCNNGC	7 cut(s)	323, 514, 549, 582, 814, 1023, 1217
BstDEI	CTNAG	4 cut(s)	138, 317, 504, 731
BstF5I	GGATG	3 cut(s)	569, 832, 868
BstFNI	CGCG	1 cut(s)	311
BstHHI	GCGC	1 cut(s)	313
BstKTI	GATC	6 cut(s)	31, 70, 469, 501, 1170, 1207
BstMAI	GTCTC	3 cut(s)	607, 734, 985
BstMBI	GATC	6 cut(s)	28, 67, 466, 498, 1167, 1204
BstMWI	GCNNNNNNNGC	5 cut(s)	338, 421, 518, 1003, 1018
BstNI	CCWGG	2 cut(s)	281, 1149
BstNSI	RCATGY	1 cut(s)	325
BstSCI	CCNGG	2 cut(s)	279, 1147
BstSFI	CTRYAG	2 cut(s)	59, 519
BstUI	CGCG	1 cut(s)	311
BstV1I	GCAGC	6 cut(s)	134, 256, 350, 408, 774, 1187
BstX2I	RGATCY	2 cut(s)	498, 1167
BstYI	RGATCY	2 cut(s)	498, 1167
BsuRI	GGCC	1 cut(s)	252
BtsCI	GGATG	3 cut(s)	569, 832, 868
BtsI	GCAGTG	1 cut(s)	807
BtsIMutI	CAGTG	2 cut(s)	739, 807
Cac8I	GCNNGC	7 cut(s)	323, 514, 549, 582, 814, 1023, 1217
CfoI	GCGC	1 cut(s)	313
CseI	GACGC	1 cut(s)	160
Csp6I	GTAC	2 cut(s)	187, 529
CviAII	CATG	1 cut(s)	322
CviQI	GTAC	2 cut(s)	187, 529
DdeI	CTNAG	4 cut(s)	138, 317, 504, 731
DpnI	GATC	6 cut(s)	30, 69, 468, 500, 1169, 1206
DpnII	GATC	6 cut(s)	28, 67, 466, 498, 1167, 1204
Eam1104I	CTCTTC	3 cut(s)	39, 348, 825
EarI	CTCTTC	3 cut(s)	39, 348, 825
EciI	GGCGGA	1 cut(s)	429
Ecl136II	GAGCTC	1 cut(s)	364
Eco24I	GRGCYC	1 cut(s)	366
Eco53kI	GAGCTC	1 cut(s)	364
EcoICRI	GAGCTC	1 cut(s)	364
EcoRII	CCWGG	2 cut(s)	279, 1147
EcoT38I	GRGCYC	1 cut(s)	366
FaeI	CATG	1 cut(s)	325
FaiI	YATR	8 cut(s)	200, 323, 380, 474, 483, 596, 1245, 1264
FaqI	GGGAC	1 cut(s)	274
FatI	CATG	1 cut(s)	321
FauI	CCCGC	1 cut(s)	488
Fnu4HI	GCNGC	6 cut(s)	123, 270, 339, 422, 763, 1176
FokI	GGATG	3 cut(s)	556, 839, 875
FriOI	GRGCYC	1 cut(s)	366
Fsp4HI	GCNGC	6 cut(s)	123, 270, 339, 422, 763, 1176
FspBI	CTAG	3 cut(s)	15, 254, 854
GlaI	GCGC	1 cut(s)	312
GluI	GCNGC	6 cut(s)	123, 270, 339, 422, 763, 1176
GsaI	CCCAGC	1 cut(s)	1127
GsuI	CTGGAG	1 cut(s)	963
HaeIII	GGCC	1 cut(s)	252
HapII	CCGG	2 cut(s)	628, 1026
HgaI	GACGC	1 cut(s)	160
HhaI	GCGC	1 cut(s)	313
Hin1I	GRCGYC	1 cut(s)	152
Hin1II	CATG	1 cut(s)	325
Hin6I	GCGC	1 cut(s)	311
HinP1I	GCGC	1 cut(s)	311
HincII	GTYRAC	1 cut(s)	669
HindII	GTYRAC	1 cut(s)	669
HindIII	AAGCTT	1 cut(s)	1188
HinfI	GANTC	3 cut(s)	215, 707, 727
HpaII	CCGG	2 cut(s)	628, 1026
HphI	GGTGA	4 cut(s)	476, 786, 941, 1057
Hpy166II	GTNNAC	5 cut(s)	187, 299, 535, 669, 1083
Hpy188I	TCNGA	4 cut(s)	33, 139, 150, 450
Hpy188III	TCNNGA	7 cut(s)	41, 173, 212, 408, 612, 923, 988
Hpy8I	GTNNAC	5 cut(s)	187, 299, 535, 669, 1083
HpyAV	CCTTC	1 cut(s)	1002
HpyCH4III	ACNGT	4 cut(s)	24, 457, 802, 1045
HpyCH4IV	ACGT	1 cut(s)	531
HpyCH4V	TGCA	6 cut(s)	61, 89, 164, 338, 521, 1175
HpyF10VI	GCNNNNNNNGC	5 cut(s)	338, 421, 518, 1003, 1018
HpyF3I	CTNAG	4 cut(s)	138, 317, 504, 731
HpySE526I	ACGT	1 cut(s)	531
Hsp92I	GRCGYC	1 cut(s)	152
Hsp92II	CATG	1 cut(s)	325
HspAI	GCGC	1 cut(s)	311
Kzo9I	GATC	6 cut(s)	28, 67, 466, 498, 1167, 1204
LguI	GCTCTTC	1 cut(s)	348
LmnI	GCTCC	5 cut(s)	104, 361, 676, 1107, 1127
Lsp1109I	GCAGC	6 cut(s)	134, 256, 350, 408, 774, 1187
LweI	GCATC	1 cut(s)	1228
MaeI	CTAG	3 cut(s)	15, 254, 854
MaeII	ACGT	1 cut(s)	531
MaeIII	GTNAC	3 cut(s)	18, 929, 1180
MalI	GATC	6 cut(s)	30, 69, 468, 500, 1169, 1206
MboI	GATC	6 cut(s)	28, 67, 466, 498, 1167, 1204
MfeI	CAATTG	1 cut(s)	1065
MflI	RGATCY	2 cut(s)	498, 1167
MhlI	GDGCHC	2 cut(s)	366, 1132
MluCI	AATT	8 cut(s)	115, 159, 770, 912, 971, 1065, 1115, 1255
MlyI	GAGTC	1 cut(s)	736
MmeI	TCCRAC	1 cut(s)	962
MroXI	GAANNNNTTC	1 cut(s)	1255
MseI	TTAA	3 cut(s)	398, 1163, 1200
MspA1I	CMGCKG	3 cut(s)	221, 269, 1178
MspI	CCGG	2 cut(s)	628, 1026
MspR9I	CCNGG	2 cut(s)	281, 1149
MunI	CAATTG	1 cut(s)	1065
MvaI	CCWGG	2 cut(s)	281, 1149
MvnI	CGCG	1 cut(s)	311
MwoI	GCNNNNNNNGC	5 cut(s)	338, 421, 518, 1003, 1018
NdeII	GATC	6 cut(s)	28, 67, 466, 498, 1167, 1204
NlaIII	CATG	1 cut(s)	325
NlaIV	GGNNCC	4 cut(s)	195, 626, 876, 1169
NmeAIII	GCCGAG	1 cut(s)	228
NmuCI	GTSAC	3 cut(s)	18, 929, 1180
NspI	RCATGY	1 cut(s)	325
PaeI	GCATGC	1 cut(s)	325
PciSI	GCTCTTC	1 cut(s)	348
PdmI	GAANNNNTTC	1 cut(s)	1255
PfeI	GAWTC	2 cut(s)	215, 707
PkrI	GCNGC	6 cut(s)	124, 271, 340, 423, 764, 1177
PleI	GAGTC	1 cut(s)	735
PpsI	GAGTC	1 cut(s)	735
Psp124BI	GAGCTC	1 cut(s)	366
Psp6I	CCWGG	2 cut(s)	279, 1147
PspFI	CCCAGC	1 cut(s)	1123
PspGI	CCWGG	2 cut(s)	279, 1147
PspN4I	GGNNCC	4 cut(s)	195, 626, 876, 1169
PstI	CTGCAG	2 cut(s)	63, 523
PsuI	RGATCY	2 cut(s)	498, 1167
PvuII	CAGCTG	1 cut(s)	269
RsaI	GTAC	2 cut(s)	188, 530
RsaNI	GTAC	2 cut(s)	187, 529
SacI	GAGCTC	1 cut(s)	366
SapI	GCTCTTC	1 cut(s)	348
SaqAI	TTAA	3 cut(s)	398, 1163, 1200
SatI	GCNGC	6 cut(s)	123, 270, 339, 422, 763, 1176
Sau3AI	GATC	6 cut(s)	28, 67, 466, 498, 1167, 1204
SchI	GAGTC	1 cut(s)	736
ScrFI	CCNGG	2 cut(s)	281, 1149
SduI	GDGCHC	2 cut(s)	366, 1132
SfaNI	GCATC	1 cut(s)	1228
SfcI	CTRYAG	2 cut(s)	59, 519
SmlI	CTYRAG	2 cut(s)	77, 988
SmoI	CTYRAG	2 cut(s)	77, 988
SphI	GCATGC	1 cut(s)	325
Sse9I	AATT	8 cut(s)	115, 159, 770, 912, 971, 1065, 1115, 1255
SsiI	CCGC	7 cut(s)	221, 440, 495, 510, 684, 824, 1178
SspI	AATATT	1 cut(s)	661
SspMI	CTAG	3 cut(s)	15, 254, 854
SstI	GAGCTC	1 cut(s)	366
StyD4I	CCNGG	2 cut(s)	279, 1147
TaaI	ACNGT	4 cut(s)	24, 457, 802, 1045
TaiI	ACGT	1 cut(s)	534
TaqI	TCGA	4 cut(s)	276, 611, 702, 1094
TasI	AATT	8 cut(s)	115, 159, 770, 912, 971, 1065, 1115, 1255
TfiI	GAWTC	2 cut(s)	215, 707
Tru1I	TTAA	3 cut(s)	398, 1163, 1200
Tru9I	TTAA	3 cut(s)	398, 1163, 1200
TscAI	CASTG	2 cut(s)	739, 814
TseFI	GTSAC	3 cut(s)	18, 929, 1180
TseI	GCWGC	6 cut(s)	122, 269, 338, 421, 762, 1175
Tsp45I	GTSAC	3 cut(s)	18, 929, 1180
TspDTI	ATGAA	2 cut(s)	17, 843
TspGWI	ACGGA	3 cut(s)	91, 1160, 1199
TspRI	CASTG	2 cut(s)	739, 814
XapI	RAATTY	3 cut(s)	115, 971, 1115
XceI	RCATGY	1 cut(s)	325
XmnI	GAANNNNTTC	1 cut(s)	1255
XspI	CTAG	3 cut(s)	15, 254, 854

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.