MD06G1222000.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0028294
Physical Location & Seq
Reverse (-)
35371680 .. 35374044
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1222000.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGGATTGGATAGCTTAAACTTGAACTATTATCTGGTGCCGGCGGGACTGTTTGTGCTGGGAATATACCACGTTTGGCTACTCATCACAGTGCTATACAATCCCTCACGAACTGTCATTGGCCTCAATGCTGTGGTTCGCACGCAATGGATCTTCTCCATGATGACTGACCCCTCGAAGAATGGAGTTTTGACGGTACAAACATTGCGCAACAACATAATGGCATCTTCCCTTTTGGCAACAGCAGCAATCACTCTTTCTTCGGTAATCAGCGTATTCGTGAGCACCAGTTCGAGTTCCGAAAGCACTTCAACACAAAAACTTTCGTCTCTCCCTCTCTCTTCAATCAAATACTTATGCGTTTTGCTGTGTTTTCTCGTTGGATTTATCTGCAATGTGCTGGCCACTAGATACTTTGCTCATACCAGCTTCTTGGCTACCCTGCCAGTATGGACAGACAAAACAGATTACGTTAAATATGTTTCGGTAACTTTGAATCGAGGGAGTTACTTTTGGTCCCTCGGATTGCGAGCGTTTTACCTCTCAATTCCTATGTTTCTTTGGATCTTTGGGCCAATTCCCATGTTTGTCTGTTGCTGCCTTATGTCAAGTGCCCTCTATTTCTTGGACACTACCACCAGTTCAACACAACTTGTTCTCGGCTCTTCACTCAAAGAAGAAGGGGAGAGAACCAATGATGTGGAATCAATAGTTCAATCATCGGAAAACTCGGGCCGTGAGGACTTTACTCACCTTCGCAGCCCCCTACTTACTGCCGATTCAGCTTCTGCCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

266

Amino Acids

29.19

Weight (kDa)

6.8

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 12 - 213 1.4e-60 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028294)

Species Orthologous Gene IDs
malus_domestica MD06G1222000.v1.1
pyrus_communis pycom06g19750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 37
AciI CCGC 1 cut(s) 44
AclWI GGATC 2 cut(s) 158, 572
AcoI YGGCCR 1 cut(s) 402
AfaI GTAC 1 cut(s) 198
AgsI TTSAA 6 cut(s) 25, 312, 345, 496, 645, 716
AluBI AGCT 3 cut(s) 15, 429, 785
AluI AGCT 3 cut(s) 15, 429, 785
Alw21I GWGCWC 1 cut(s) 287
Alw26I GTCTC 1 cut(s) 333
AlwI GGATC 2 cut(s) 158, 572
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 1 cut(s) 730
AoxI GGCC 4 cut(s) 121, 402, 572, 733
ApeKI GCWGC 3 cut(s) 245, 597, 759
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 3 cut(s) 516, 572, 733
AsuHPI GGTGA 1 cut(s) 743
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 1 cut(s) 516
BaeGI GKGCMC 1 cut(s) 616
BalI TGGCCA 1 cut(s) 404
BanI GGYRCC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 287
BbvI GCAGC 3 cut(s) 257, 584, 771
BceAI ACGGC 1 cut(s) 720
BcoDI GTCTC 1 cut(s) 333
BfaI CTAG 1 cut(s) 408
BisI GCNGC 3 cut(s) 246, 598, 760
BlsI GCNGC 3 cut(s) 247, 599, 761
Bme18I GGWCC 1 cut(s) 516
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 3 cut(s) 516, 572, 733
BmiI GGNNCC 2 cut(s) 39, 518
BmsI GCATC 1 cut(s) 233
BsaJI CCNNGG 1 cut(s) 520
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 3 cut(s) 288, 446, 639
Bse3DI GCAATG 3 cut(s) 152, 203, 400
BseDI CCNNGG 1 cut(s) 520
BseMI GCAATG 3 cut(s) 152, 203, 400
BseNI ACTGG 3 cut(s) 288, 446, 639
BseSI GKGCMC 1 cut(s) 616
BseXI GCAGC 3 cut(s) 257, 584, 771
BseYI CCCAGC 1 cut(s) 58
BshFI GGCC 4 cut(s) 123, 404, 574, 735
BshNI GGYRCC 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 287
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 1 cut(s) 41
BslFI GGGAC 2 cut(s) 60, 502
BsmAI GTCTC 1 cut(s) 333
BsmBI CGTCTC 1 cut(s) 333
BsmFI GGGAC 2 cut(s) 60, 502
BsnI GGCC 4 cut(s) 123, 404, 574, 735
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 2 cut(s) 287, 616
Bsp143I GATC 2 cut(s) 150, 564
BspACI CCGC 1 cut(s) 44
BspANI GGCC 4 cut(s) 123, 404, 574, 735
BspLI GGNNCC 2 cut(s) 39, 518
BspPI GGATC 2 cut(s) 158, 572
BspQI GCTCTTC 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 37
BsrDI GCAATG 3 cut(s) 152, 203, 400
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 3 cut(s) 288, 446, 639
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 1 cut(s) 520
BssMI GATC 2 cut(s) 150, 564
Bst4CI ACNGT 4 cut(s) 51, 91, 115, 196
Bst6I CTCTTC 2 cut(s) 346, 670
BstC8I GCNNGC 4 cut(s) 42, 143, 402, 531
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 2 cut(s) 153, 567
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 2 cut(s) 150, 564
BstMWI GCNNNNNNNGC 2 cut(s) 245, 782
BstSLI GKGCMC 1 cut(s) 616
BstV1I GCAGC 3 cut(s) 257, 584, 771
BstX2I RGATCY 2 cut(s) 150, 564
BstXI CCANNNNNNTGG 2 cut(s) 433, 700
BstYI RGATCY 2 cut(s) 150, 564
BsuRI GGCC 4 cut(s) 123, 404, 574, 735
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 4 cut(s) 42, 143, 402, 531
CaiI CAGNNNCTG 1 cut(s) 788
CfoI GCGC 1 cut(s) 210
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 516, 572, 733
Csp6I GTAC 1 cut(s) 197
CviAII CATG 2 cut(s) 160, 583
CviQI GTAC 1 cut(s) 197
DpnI GATC 2 cut(s) 152, 566
DpnII GATC 2 cut(s) 150, 564
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 2 cut(s) 346, 670
EarI CTCTTC 2 cut(s) 346, 670
Eco47I GGWCC 1 cut(s) 516
Eco88I CYCGRG 1 cut(s) 730
Esp3I CGTCTC 1 cut(s) 333
FaeI CATG 2 cut(s) 163, 586
FaqI GGGAC 2 cut(s) 60, 502
FatI CATG 2 cut(s) 159, 582
FauI CCCGC 1 cut(s) 37
Fnu4HI GCNGC 3 cut(s) 246, 598, 760
Fsp4HI GCNGC 3 cut(s) 246, 598, 760
FspBI CTAG 1 cut(s) 408
FspI TGCGCA 1 cut(s) 209
GlaI GCGC 1 cut(s) 209
GluI GCNGC 3 cut(s) 246, 598, 760
GsaI CCCAGC 1 cut(s) 62
HaeIII GGCC 4 cut(s) 123, 404, 574, 735
HapII CCGG 1 cut(s) 41
HhaI GCGC 1 cut(s) 210
Hin1II CATG 2 cut(s) 163, 586
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HinfI GANTC 3 cut(s) 496, 704, 779
HpaII CCGG 1 cut(s) 41
HphI GGTGA 1 cut(s) 743
Hpy188I TCNGA 3 cut(s) 301, 524, 724
Hpy188III TCNNGA 2 cut(s) 108, 280
HpyAV CCTTC 2 cut(s) 674, 764
HpyCH4III ACNGT 4 cut(s) 51, 91, 115, 196
HpyCH4IV ACGT 2 cut(s) 72, 471
HpyCH4V TGCA 1 cut(s) 393
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 782
HpySE526I ACGT 2 cut(s) 72, 471
Hsp92II CATG 2 cut(s) 163, 586
HspAI GCGC 1 cut(s) 208
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Kzo9I GATC 2 cut(s) 150, 564
LguI GCTCTTC 1 cut(s) 670
LpnPI CCDG 9 cut(s) 20, 44, 54, 301, 386, 439, 455, 459, 652
Lsp1109I GCAGC 3 cut(s) 257, 584, 771
LweI GCATC 1 cut(s) 233
MaeI CTAG 1 cut(s) 408
MaeII ACGT 2 cut(s) 72, 471
MaeIII GTNAC 2 cut(s) 487, 506
MalI GATC 2 cut(s) 152, 566
MboI GATC 2 cut(s) 150, 564
MboII GAAGA 7 cut(s) 145, 190, 219, 252, 333, 657, 689
MflI RGATCY 2 cut(s) 150, 564
MhlI GDGCHC 2 cut(s) 287, 616
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 2 cut(s) 546, 576
MluNI TGGCCA 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 361
MnlI CCTC 9 cut(s) 115, 134, 184, 345, 494, 530, 551, 626, 733
Mox20I TGGCCA 1 cut(s) 404
MroNI GCCGGC 1 cut(s) 40
MscI TGGCCA 1 cut(s) 404
MseI TTAA 3 cut(s) 17, 474, 796
MslI CAYNNNNRTG 1 cut(s) 89
Msp20I TGGCCA 1 cut(s) 404
MspI CCGG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 245, 782
NaeI GCCGGC 1 cut(s) 42
NdeII GATC 2 cut(s) 150, 564
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 2 cut(s) 163, 586
NlaIV GGNNCC 2 cut(s) 39, 518
NmeAIII GCCGAG 1 cut(s) 639
NsbI TGCGCA 1 cut(s) 209
PciSI GCTCTTC 1 cut(s) 670
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 3 cut(s) 496, 704, 779
PkrI GCNGC 3 cut(s) 247, 599, 761
PspFI CCCAGC 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 39, 518
PspPI GGNCC 3 cut(s) 516, 572, 733
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 2 cut(s) 150, 564
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
RseI CAYNNNNRTG 1 cut(s) 89
SapI GCTCTTC 1 cut(s) 670
SaqAI TTAA 3 cut(s) 17, 474, 796
SatI GCNGC 3 cut(s) 246, 598, 760
Sau3AI GATC 2 cut(s) 150, 564
Sau96I GGNCC 3 cut(s) 516, 572, 733
SduI GDGCHC 2 cut(s) 287, 616
SetI ASST 7 cut(s) 17, 75, 431, 474, 543, 756, 787
SfaNI GCATC 1 cut(s) 233
SinI GGWCC 1 cut(s) 516
SmiMI CAYNNNNRTG 1 cut(s) 89
Sse9I AATT 2 cut(s) 546, 576
SsiI CCGC 1 cut(s) 44
SspMI CTAG 1 cut(s) 408
TaaI ACNGT 4 cut(s) 51, 91, 115, 196
TaiI ACGT 2 cut(s) 75, 474
TaqI TCGA 3 cut(s) 176, 293, 499
TasI AATT 2 cut(s) 546, 576
TfiI GAWTC 3 cut(s) 496, 704, 779
Tru1I TTAA 3 cut(s) 17, 474, 796
Tru9I TTAA 3 cut(s) 17, 474, 796
TscAI CASTG 1 cut(s) 96
TseI GCWGC 3 cut(s) 245, 597, 759
TspRI CASTG 1 cut(s) 96
VpaK11BI GGWCC 1 cut(s) 516
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.