pycom06g19750

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0028294
Physical Location & Seq
Reverse (-)
23079184 .. 23081038
1855 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19750.3

Sequence Viewer

Length: 807 bp
ATGAGGGATATGGGATTGGATAGCTTAAGCTTGAACTATTATCTGGTGCCGGCGGGACTGTTTGTGCTGGGAATATACCACGTTTGGCTACTCATCACAGTGCTATGCAATCCCTCACGAACTGTCATTGGCCTCAATGCTGTGGTTCGCACGCAATGGATCTTCTCCATGATGACTGACCCCTCGAAGAATGGAGTTTTGATGGTACAAACATTGCGCAACAACATAATGGCGTCTTCCCTTTTGGCAACAGCAGCAATCACTCTTTCTTCGGTAATCAGCGTATTCGTGAGCACCAGTTCGAGCTTCGAAAGCACTTCAACACAAAAACTTTCCTCTCTCCCTCTCTCTTCAATCAAGTACTTCTGCGTTTTACTGTGTTTTCTCGTTGGATTTATCTGCAATGTGCTGGCCACTAGATACTTTGCTCATACCAGCTTCTTGGCTACCCTGCCAGTATGGACAGACAAAACAGACTATGTTGAATATGTTGCGATAACTTTGAATCGAGGAAGTTACTTTTGGTCCTTCGGATTGCGGGCGTTTTACCTCTCAATTCCCATGTTTCTTTGGATCTTTGGGCCAATTGCCATGTTTGTCTGTTGCTGCCTTATGTCAAGTGCCCTCTATTTCTTGGACACTACCACCAGTTCAACACAACTTGTTCTCGGCTCTTCACTCAAAGAAGAAAGGGAGAGAACCAATGATGTGGAATCAATTGTTCAATCATCGGAAAACTCGGGCCGTGAGGACTTTAATCACCTTCGCTTCCCCCTACTTACTGCCGATTCAGCTTCTGCCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

269

Amino Acids

29.81

Weight (kDa)

6.28

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028294)

Species Orthologous Gene IDs
malus_domestica MD06G1222000.v1.1
pyrus_communis pycom06g19750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 46
AciI CCGC 2 cut(s) 53, 538
AclWI GGATC 2 cut(s) 167, 581
AcoI YGGCCR 1 cut(s) 411
AcyI GRCGYC 1 cut(s) 233
AfaI GTAC 2 cut(s) 207, 362
AflII CTTAAG 1 cut(s) 25
AgsI TTSAA 7 cut(s) 34, 321, 354, 485, 505, 654, 725
AjuI GAANNNNNNNTTGG 2 cut(s) 505, 537
AluBI AGCT 5 cut(s) 24, 30, 306, 438, 794
AluI AGCT 5 cut(s) 24, 30, 306, 438, 794
Alw21I GWGCWC 1 cut(s) 296
AlwI GGATC 2 cut(s) 167, 581
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 1 cut(s) 739
AoxI GGCC 4 cut(s) 130, 411, 581, 742
ApeKI GCWGC 2 cut(s) 254, 606
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 3 cut(s) 525, 581, 742
AsuHPI GGTGA 1 cut(s) 752
AsuII TTCGAA 1 cut(s) 309
AvaI CYCGRG 1 cut(s) 739
AvaII GGWCC 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 625
BalI TGGCCA 1 cut(s) 413
BanI GGYRCC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 266, 593
BccI CCATC 1 cut(s) 196
BceAI ACGGC 1 cut(s) 729
BfaI CTAG 1 cut(s) 417
BfrI CTTAAG 1 cut(s) 25
BisI GCNGC 2 cut(s) 255, 607
BlsI GCNGC 2 cut(s) 256, 608
BmcAI AGTACT 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 525
BmeT110I CYCGRG 1 cut(s) 739
BmgT120I GGNCC 3 cut(s) 525, 581, 742
BmiI GGNNCC 1 cut(s) 48
BpiI GAAGAC 1 cut(s) 228
Bpu14I TTCGAA 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 233
Bse118I RCCGGY 1 cut(s) 49
Bse1I ACTGG 3 cut(s) 297, 455, 648
Bse3DI GCAATG 3 cut(s) 161, 212, 409
BseMI GCAATG 3 cut(s) 161, 212, 409
BseNI ACTGG 3 cut(s) 297, 455, 648
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 2 cut(s) 266, 593
BseYI CCCAGC 1 cut(s) 67
BshFI GGCC 4 cut(s) 132, 413, 583, 744
BshNI GGYRCC 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 296
BsiHKCI CYCGRG 1 cut(s) 739
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 4 cut(s) 132, 413, 583, 744
BsoBI CYCGRG 1 cut(s) 739
Bsp119I TTCGAA 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 296, 625
Bsp143I GATC 2 cut(s) 159, 573
BspACI CCGC 2 cut(s) 53, 538
BspANI GGCC 4 cut(s) 132, 413, 583, 744
BspLI GGNNCC 1 cut(s) 48
BspPI GGATC 2 cut(s) 167, 581
BspQI GCTCTTC 1 cut(s) 679
BspT104I TTCGAA 1 cut(s) 309
BspT107I GGYRCC 1 cut(s) 46
BspTI CTTAAG 1 cut(s) 25
BsrDI GCAATG 3 cut(s) 161, 212, 409
BsrFI RCCGGY 1 cut(s) 49
BsrI ACTGG 3 cut(s) 297, 455, 648
BssAI RCCGGY 1 cut(s) 49
BssMI GATC 2 cut(s) 159, 573
BssNI GRCGYC 1 cut(s) 233
Bst4CI ACNGT 4 cut(s) 60, 100, 124, 378
Bst6I CTCTTC 2 cut(s) 355, 679
BstACI GRCGYC 1 cut(s) 233
BstAFI CTTAAG 1 cut(s) 25
BstBI TTCGAA 1 cut(s) 309
BstC8I GCNNGC 4 cut(s) 51, 152, 411, 540
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 2 cut(s) 162, 576
BstMBI GATC 2 cut(s) 159, 573
BstMWI GCNNNNNNNGC 3 cut(s) 254, 312, 791
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 2 cut(s) 266, 593
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 2 cut(s) 159, 573
BstXI CCANNNNNNTGG 2 cut(s) 442, 709
BstYI RGATCY 2 cut(s) 159, 573
BsuRI GGCC 4 cut(s) 132, 413, 583, 744
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 4 cut(s) 51, 152, 411, 540
CaiI CAGNNNCTG 1 cut(s) 797
CfoI GCGC 1 cut(s) 219
Cfr10I RCCGGY 1 cut(s) 49
Cfr13I GGNCC 3 cut(s) 525, 581, 742
CseI GACGC 1 cut(s) 222
Csp6I GTAC 2 cut(s) 206, 361
CviAII CATG 3 cut(s) 169, 562, 592
CviQI GTAC 2 cut(s) 206, 361
DpnI GATC 2 cut(s) 161, 575
DpnII GATC 2 cut(s) 159, 573
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 2 cut(s) 355, 679
EarI CTCTTC 2 cut(s) 355, 679
Eco47I GGWCC 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 739
FaeI CATG 3 cut(s) 172, 565, 595
FaqI GGGAC 1 cut(s) 69
FatI CATG 3 cut(s) 168, 561, 591
FauI CCCGC 2 cut(s) 46, 531
Fnu4HI GCNGC 2 cut(s) 255, 607
Fsp4HI GCNGC 2 cut(s) 255, 607
FspBI CTAG 1 cut(s) 417
FspI TGCGCA 1 cut(s) 218
GlaI GCGC 1 cut(s) 218
GluI GCNGC 2 cut(s) 255, 607
GsaI CCCAGC 1 cut(s) 71
HaeIII GGCC 4 cut(s) 132, 413, 583, 744
HapII CCGG 1 cut(s) 50
HgaI GACGC 1 cut(s) 222
HhaI GCGC 1 cut(s) 219
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 3 cut(s) 172, 565, 595
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 3 cut(s) 505, 713, 788
HpaII CCGG 1 cut(s) 50
HphI GGTGA 1 cut(s) 752
Hpy188I TCNGA 2 cut(s) 533, 733
Hpy188III TCNNGA 2 cut(s) 117, 289
HpyAV CCTTC 2 cut(s) 538, 773
HpyCH4III ACNGT 4 cut(s) 60, 100, 124, 378
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 2 cut(s) 108, 402
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 312, 791
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 3 cut(s) 172, 565, 595
HspAI GCGC 1 cut(s) 217
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
Kzo9I GATC 2 cut(s) 159, 573
LguI GCTCTTC 1 cut(s) 679
LpnPI CCDG 9 cut(s) 29, 53, 63, 310, 395, 448, 464, 468, 661
Lsp1109I GCAGC 2 cut(s) 266, 593
MaeI CTAG 1 cut(s) 417
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 515
MalI GATC 2 cut(s) 161, 575
MboI GATC 2 cut(s) 159, 573
MboII GAAGA 7 cut(s) 154, 199, 228, 261, 342, 666, 698
MfeI CAATTG 2 cut(s) 585, 717
MflI RGATCY 2 cut(s) 159, 573
MhlI GDGCHC 2 cut(s) 296, 625
MlsI TGGCCA 1 cut(s) 413
MluCI AATT 3 cut(s) 555, 585, 717
MluNI TGGCCA 1 cut(s) 413
MmeI TCCRAC 1 cut(s) 370
MnlI CCTC 9 cut(s) 124, 143, 193, 346, 354, 503, 560, 635, 742
Mox20I TGGCCA 1 cut(s) 413
MroNI GCCGGC 1 cut(s) 49
MscI TGGCCA 1 cut(s) 413
MseI TTAA 3 cut(s) 26, 756, 805
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 413
MspCI CTTAAG 1 cut(s) 25
MspI CCGG 1 cut(s) 50
MunI CAATTG 2 cut(s) 585, 717
MwoI GCNNNNNNNGC 3 cut(s) 254, 312, 791
NaeI GCCGGC 1 cut(s) 51
NdeII GATC 2 cut(s) 159, 573
NgoMIV GCCGGC 1 cut(s) 49
NlaIII CATG 3 cut(s) 172, 565, 595
NlaIV GGNNCC 1 cut(s) 48
NmeAIII GCCGAG 1 cut(s) 648
NsbI TGCGCA 1 cut(s) 218
NspV TTCGAA 1 cut(s) 309
PciSI GCTCTTC 1 cut(s) 679
PdiI GCCGGC 1 cut(s) 51
PfeI GAWTC 3 cut(s) 505, 713, 788
PkrI GCNGC 2 cut(s) 256, 608
PspFI CCCAGC 1 cut(s) 67
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 3 cut(s) 525, 581, 742
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 2 cut(s) 159, 573
RsaI GTAC 2 cut(s) 207, 362
RsaNI GTAC 2 cut(s) 206, 361
RseI CAYNNNNRTG 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 679
SaqAI TTAA 3 cut(s) 26, 756, 805
SatI GCNGC 2 cut(s) 255, 607
Sau3AI GATC 2 cut(s) 159, 573
Sau96I GGNCC 3 cut(s) 525, 581, 742
ScaI AGTACT 1 cut(s) 362
SduI GDGCHC 2 cut(s) 296, 625
SetI ASST 8 cut(s) 26, 32, 84, 308, 440, 552, 765, 796
SfuI TTCGAA 1 cut(s) 309
SinI GGWCC 1 cut(s) 525
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
Sse9I AATT 3 cut(s) 555, 585, 717
SsiI CCGC 2 cut(s) 53, 538
SspMI CTAG 1 cut(s) 417
TaaI ACNGT 4 cut(s) 60, 100, 124, 378
TaiI ACGT 1 cut(s) 84
TaqI TCGA 4 cut(s) 185, 302, 309, 508
TasI AATT 3 cut(s) 555, 585, 717
TatI WGTACW 1 cut(s) 360
TfiI GAWTC 3 cut(s) 505, 713, 788
Tru1I TTAA 3 cut(s) 26, 756, 805
Tru9I TTAA 3 cut(s) 26, 756, 805
TscAI CASTG 1 cut(s) 105
TseI GCWGC 2 cut(s) 254, 606
TspRI CASTG 1 cut(s) 105
Vha464I CTTAAG 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 525
XspI CTAG 1 cut(s) 417
ZrmI AGTACT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.