MD06G1229000.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0022317
Physical Location & Seq
Reverse (-)
35885598 .. 35886233
636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1229000.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGTGGAGGACTTGTCTAACAAAGGCAAGTTGAAGAATTGCTTGGCTGTATGTGAAGTCACGGGAACCTTGAGTTGGGTGTCGGTGGCTATGGGGCTATTGGTGTCTCAACTGAGTGATCACGAGCCCTGGAAAGGAAAGGTCATCCCTTTCACTCGTAATCCTGATAAGCTCCATCTCATACAAGGCAATGATCTCGAGTCTAAGCGCGCCTTCTTGAGCGACATGAAAGGCCTTGGAGATAGCACTACAACTCAAAAGGTGCTTAATTTGATTCTCCAGGAGGCCTTGAATGCTAATTTGAAGCCGGAACATATGATCAAGAAGGTGCTAGTTTTTGCCCACTTGGACTTTGATATGTCTTCCAGCCACGCGGATCACTGGCCGATTACTTATCAGGCAATGAGGAGCAAGTTTGAAGAGAAAGGCTATGTGGTGCCACACATAGTGTTCTGGGACTTGTCTCATGACAACAAGATCAAATGTTCACAACTATCAGGGATGACCACCTATAGTGGCTACTCCGATGATTTCCTCAAGTTGTTCTTGGATAGGGACGGGGATGTCGATCAGGATCATGTCATGGAAGCAGCAATATGTAGAGAAGAGTACCAAAATCTGGTTGTAGTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

212

Amino Acids

23.9

Weight (kDa)

5.55

Isoelectric Point (pI)

22.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7788 PF25043 13 - 194 6.6e-44 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022317)

Species Orthologous Gene IDs
malus_domestica MD00G1160800.v1.1 MD00G1160900.v1.1 MD06G1229000.v1.1
pyrus_communis pycom06g20020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 563
AccB1I GGYRCC 1 cut(s) 436
AccB7I CCANNNNNTGG 1 cut(s) 619
AccI GTMKAC 1 cut(s) 630
AccII CGCG 2 cut(s) 210, 374
AciI CCGC 1 cut(s) 374
AclWI GGATC 2 cut(s) 384, 582
AcoI YGGCCR 1 cut(s) 383
AdeI CACNNNGTG 1 cut(s) 448
AfaI GTAC 1 cut(s) 611
AfiI CCNNNNNNNGG 3 cut(s) 75, 134, 619
AgsI TTSAA 4 cut(s) 34, 292, 304, 419
AjnI CCWGG 2 cut(s) 128, 279
AjuI GAANNNNNNNTTGG 4 cut(s) 26, 58, 58, 90
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
Alw26I GTCTC 2 cut(s) 111, 468
AlwI GGATC 2 cut(s) 384, 582
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 3 cut(s) 232, 285, 383
ApeKI GCWGC 1 cut(s) 590
AspLEI GCGC 2 cut(s) 210, 212
AvaI CYCGRG 1 cut(s) 197
BanI GGYRCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 129
BauI CACGAG 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 602
BccI CCATC 1 cut(s) 183
BcgI CGANNNNNNTGC 2 cut(s) 178, 212
BciT130I CCWGG 2 cut(s) 130, 281
BclI TGATCA 2 cut(s) 118, 318
BcoDI GTCTC 2 cut(s) 111, 468
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 511
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
Bme1390I CCNGG 2 cut(s) 130, 281
BmeT110I CYCGRG 1 cut(s) 197
BmiI GGNNCC 2 cut(s) 67, 438
BmrFI CCNGG 2 cut(s) 130, 281
BpiI GAAGAC 1 cut(s) 354
BpmI CTGGAG 1 cut(s) 263
BpuEI CTTGAG 3 cut(s) 91, 238, 521
BsaBI GATNNNNATC 2 cut(s) 567, 573
BsaJI CCNNGG 2 cut(s) 128, 235
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 134, 619
Bse1I ACTGG 1 cut(s) 386
Bse3DI GCAATG 2 cut(s) 196, 408
Bse8I GATNNNNATC 2 cut(s) 567, 573
BseBI CCWGG 2 cut(s) 130, 281
BseDI CCNNGG 2 cut(s) 128, 235
BseGI GGATG 3 cut(s) 144, 507, 568
BseJI GATNNNNATC 2 cut(s) 567, 573
BseLI CCNNNNNNNGG 3 cut(s) 75, 134, 619
BseMI GCAATG 2 cut(s) 196, 408
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 1 cut(s) 386
BsePI GCGCGC 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 421
BseXI GCAGC 1 cut(s) 602
Bsh1236I CGCG 2 cut(s) 210, 374
BshFI GGCC 3 cut(s) 234, 287, 385
BshNI GGYRCC 1 cut(s) 436
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 1 cut(s) 308
BslFI GGGAC 2 cut(s) 470, 569
BslI CCNNNNNNNGG 3 cut(s) 75, 134, 619
BsmAI GTCTC 2 cut(s) 111, 468
BsmFI GGGAC 2 cut(s) 470, 569
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 3 cut(s) 234, 287, 385
BsoBI CYCGRG 1 cut(s) 197
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 7 cut(s) 118, 193, 318, 376, 477, 568, 574
BspACI CCGC 1 cut(s) 374
BspANI GGCC 3 cut(s) 234, 287, 385
BspCNI CTCAG 1 cut(s) 105
BspFNI CGCG 2 cut(s) 210, 374
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 67, 438
BspPI GGATC 2 cut(s) 384, 582
BspT107I GGYRCC 1 cut(s) 436
BsrDI GCAATG 2 cut(s) 196, 408
BsrI ACTGG 1 cut(s) 386
BssECI CCNNGG 2 cut(s) 128, 235
BssHII GCGCGC 1 cut(s) 208
BssMI GATC 7 cut(s) 118, 193, 318, 376, 477, 568, 574
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 235
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 2 cut(s) 130, 281
Bst6I CTCTTC 2 cut(s) 414, 600
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 2 cut(s) 113, 204
BstF5I GGATG 3 cut(s) 144, 507, 568
BstFNI CGCG 2 cut(s) 210, 374
BstHHI GCGC 2 cut(s) 210, 212
BstKTI GATC 7 cut(s) 121, 196, 321, 379, 480, 571, 577
BstMAI GTCTC 2 cut(s) 111, 468
BstMBI GATC 7 cut(s) 118, 193, 318, 376, 477, 568, 574
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 2 cut(s) 130, 281
BstSCI CCNGG 2 cut(s) 128, 279
BstSFI CTRYAG 1 cut(s) 511
BstUI CGCG 2 cut(s) 210, 374
BstV1I GCAGC 1 cut(s) 602
BstV2I GAAGAC 1 cut(s) 354
BsuRI GGCC 3 cut(s) 234, 287, 385
BtsCI GGATG 3 cut(s) 144, 507, 568
BtsIMutI CAGTG 1 cut(s) 379
Cac8I GCNNGC 1 cut(s) 210
CciI TCATGA 1 cut(s) 466
CfoI GCGC 2 cut(s) 210, 212
Csp6I GTAC 1 cut(s) 610
CviAII CATG 4 cut(s) 226, 467, 578, 583
CviQI GTAC 1 cut(s) 610
DdeI CTNAG 2 cut(s) 113, 204
DpnI GATC 7 cut(s) 120, 195, 320, 378, 479, 570, 576
DpnII GATC 7 cut(s) 118, 193, 318, 376, 477, 568, 574
DraIII CACNNNGTG 1 cut(s) 448
DrdI GACNNNNNNGTC 1 cut(s) 563
DseDI GACNNNNNNGTC 1 cut(s) 563
EaeI YGGCCR 1 cut(s) 383
Eam1104I CTCTTC 2 cut(s) 414, 600
EarI CTCTTC 2 cut(s) 414, 600
Eco130I CCWWGG 1 cut(s) 235
Eco147I AGGCCT 2 cut(s) 234, 287
Eco24I GRGCYC 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 197
EcoRII CCWGG 2 cut(s) 128, 279
EcoT14I CCWWGG 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 4 cut(s) 229, 470, 581, 586
FalI AAGNNNNNCTT 6 cut(s) 26, 58, 197, 229, 530, 562
FaqI GGGAC 2 cut(s) 470, 569
FatI CATG 4 cut(s) 225, 466, 577, 582
FauNDI CATATG 1 cut(s) 315
FbaI TGATCA 2 cut(s) 118, 318
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 1 cut(s) 591
FokI GGATG 3 cut(s) 131, 514, 575
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 1 cut(s) 591
FspBI CTAG 1 cut(s) 332
GlaI GCGC 2 cut(s) 209, 211
GluI GCNGC 1 cut(s) 591
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 3 cut(s) 234, 287, 385
HapII CCGG 1 cut(s) 308
HhaI GCGC 2 cut(s) 210, 212
Hin1II CATG 4 cut(s) 229, 470, 581, 586
Hin6I GCGC 2 cut(s) 208, 210
HinP1I GCGC 2 cut(s) 208, 210
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 2 cut(s) 200, 274
HpaII CCGG 1 cut(s) 308
Hpy166II GTNNAC 2 cut(s) 488, 631
Hpy188I TCNGA 1 cut(s) 526
Hpy188III TCNNGA 7 cut(s) 122, 164, 197, 217, 322, 467, 572
Hpy8I GTNNAC 2 cut(s) 488, 631
HpyAV CCTTC 2 cut(s) 223, 319
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 2 cut(s) 113, 204
Hsp92II CATG 4 cut(s) 229, 470, 581, 586
HspAI GCGC 2 cut(s) 208, 210
Ksp22I TGATCA 2 cut(s) 118, 318
Kzo9I GATC 7 cut(s) 118, 193, 318, 376, 477, 568, 574
LmnI GCTCC 2 cut(s) 177, 408
Lsp1109I GCAGC 1 cut(s) 602
MaeI CTAG 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 58
MalI GATC 7 cut(s) 120, 195, 320, 378, 479, 570, 576
MboI GATC 7 cut(s) 118, 193, 318, 376, 477, 568, 574
MboII GAAGA 4 cut(s) 46, 354, 431, 617
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 3 cut(s) 37, 268, 298
MlyI GAGTC 1 cut(s) 209
MnlI CCTC 3 cut(s) 277, 399, 545
MseI TTAA 1 cut(s) 267
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 2 cut(s) 130, 281
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 2 cut(s) 130, 281
MvnI CGCG 2 cut(s) 210, 374
MwoI GCNNNNNNNGC 1 cut(s) 293
NdeI CATATG 1 cut(s) 315
NdeII GATC 7 cut(s) 118, 193, 318, 376, 477, 568, 574
NlaIII CATG 4 cut(s) 229, 470, 581, 586
NlaIV GGNNCC 2 cut(s) 67, 438
NmuCI GTSAC 1 cut(s) 58
PaeR7I CTCGAG 1 cut(s) 197
PagI TCATGA 1 cut(s) 466
PauI GCGCGC 1 cut(s) 208
PceI AGGCCT 2 cut(s) 234, 287
PcsI WCGNNNNNNNCGW 1 cut(s) 564
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 1 cut(s) 274
PflMI CCANNNNNTGG 1 cut(s) 619
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 1 cut(s) 592
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
Psp6I CCWGG 2 cut(s) 128, 279
PspGI CCWGG 2 cut(s) 128, 279
PspN4I GGNNCC 2 cut(s) 67, 438
PteI GCGCGC 1 cut(s) 208
RsaI GTAC 1 cut(s) 611
RsaNI GTAC 1 cut(s) 610
SalI GTCGAC 1 cut(s) 629
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 7 cut(s) 118, 193, 318, 376, 477, 568, 574
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 2 cut(s) 130, 281
SduI GDGCHC 1 cut(s) 129
SetI ASST 6 cut(s) 71, 144, 174, 264, 330, 512
SfcI CTRYAG 1 cut(s) 511
Sfr274I CTCGAG 1 cut(s) 197
SlaI CTCGAG 1 cut(s) 197
SmlI CTYRAG 4 cut(s) 70, 197, 217, 536
SmoI CTYRAG 4 cut(s) 70, 197, 217, 536
Sse9I AATT 3 cut(s) 37, 268, 298
SseBI AGGCCT 2 cut(s) 234, 287
SsiI CCGC 1 cut(s) 374
SspMI CTAG 1 cut(s) 332
StuI AGGCCT 2 cut(s) 234, 287
StyD4I CCNGG 2 cut(s) 128, 279
StyI CCWWGG 1 cut(s) 235
TaqI TCGA 3 cut(s) 198, 567, 630
TasI AATT 3 cut(s) 37, 268, 298
TfiI GAWTC 1 cut(s) 274
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 386
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 1 cut(s) 590
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 1 cut(s) 242
TspRI CASTG 1 cut(s) 386
Van91I CCANNNNNTGG 1 cut(s) 619
XhoI CTCGAG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 630
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.