MD07G1029300.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0024471
Physical Location & Seq
Reverse (-)
2452834 .. 2454738
1905 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1029300.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGGCTGAAGGTTTTTTGTCACCCCAAAGAAAGAAAATGATGGAAGACGTTGGAGTGGAGTACTTTGATGATTTAATATCGAGGTCATTTTTTCAGCATTCAGGTGATGACCAATCACTTTTTACCATGCACGACCTTATCAACGATTTAGCAAAGTTTGTATCAAGAGAATTTTGTTTTAGGTTGGATGATAGCGACTCATTCAATAATGTTTCAAGCAAGACTCGTCATTTCTCATACACTCAGAATAGCCCCTTTCTCGAAGGACTTGGTGTAGGTGTTCATGCGCGACTGCAAAGGAGGAGATTTCTGGAGTTGCCTTATTGGATTGGCAATTTGAAACACCTAAGGTATATAGACTTGTCATACAGTAGATTTGAAAAGTTACCTGAAACGATATGTACTTTGCATAATCTACAAACGTTGCTCTTGTTCAATTGTTATGAACTTGCTCAGTTGCCGATCAACTTGGGGAGACTCATCAACTTGCGCCATCTTGATGTTAGAAGCACTAATCTAAAAGAGATGCCACCACATATGGATAAGTTGAAGGATCTCCACACATTGAGTGACTTTGTTGTTGGCAAACAAACTGCACCTAGCATTGTAGTGTTGAAAGAGCTTCAGATAGTAGGTACACTTGCTATTTCAGGGCTTCATAATATAGTGAATTCTGAGGATGCTTTTGTTGCCAATATGAGAAATAAGCACCTTGACGGACTAGCTTTGACATGGGGAGCTGAGACCGATGATTCACAAAAAGATAGAGAGGTGCTTAATAATCTCCAGCCTCATACACACCTGAAAGCCCTCAGTCTTAAGTTTTATGGGGGTACAAGATTTCCGGATTGGTTAGGAGATCATTCTTTTTCTAATTTGGTTTCTCTTCAACTCGAAGATTGTAAACATTGTTGCGCCTTGCCACCACTGGGGCAGCTACCCTCCCTCGTCACGCTTTATGTTTGTGGGTTAAATGAAGTGGAGACGATAGGGCCTGAGTTTTATGGTAATGGTGTTTCTGTTGTTATGCCATTTAGATCTCTATCAGTTTTATTCTTCAAAGATATGTTGGGATGGCAAGAGTGGTCTCATTTCGGAAGTAGCCAAGAAGGTGGAGCTTTTCCTCATCTTTGTCAGCTCTATCTGACAAATTGTCCCAAGCTAACACAGAAATTACCTGAGTATCTTCCATCCTTGACTACACTTGAGATAAGGAGATGCGAGCAACTTCTGGGTTCACTTCCAAGAACTCGGGCCATTTTGGAGATTCCTTCTGTGAAAGACAACCTTTGTCTTGAAGAGAAAACTAGCGGTACTAACTCATCTCTTACTCCATTTCCTTGTGACGGTCTGGCTGATGCTTTAAGAGTTCTTAAAATAAAAAACTGTTGGAACTTATCTCCACTAAATTACTGCTATGCATTCCTTAAAACATTGAAGATAAAGAGTAGCTGTGATTCAACCACATCTATCCCGCTGGACTACTTCCCAAATGTTAAAGAGCTCAAATTATATGACTGTAGGAATCTGGAATCCCTTACTTATTCCCAGGATTCTGAAAGCCCTACAATCCTGTCCCTCAGTTCCTTGAGAATATTTAATTGCCCAAATTTTATATCTTTTCCTGATGGAGGACTGTACGCCCCCAACTTGATAGTGCTTAATATATCTGAATGTGATAAATTGAGGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

565

Amino Acids

64.0

Weight (kDa)

6.11

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 1 - 51 2.1e-16 Disease resistance protein Winged helix domain
LRR_14 PF23598 110 - 386 5.4e-24 Leucine-rich repeat region
LRR_Zer-1 PF25013 111 - 189 5.5e-07 Zer-1-like, Leucine-rich repeats
LRR_R13L1-DRL21 PF25019 204 - 322 1.5e-33 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024471)

Species Orthologous Gene IDs
malus_domestica MD07G1029300.v1.1
pyrus_communis pycom02g24910
rosa_rugosa Rorug01G0024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 289
AccIII TCCGGA 1 cut(s) 844
AciI CCGC 2 cut(s) 1311, 1475
AclI AACGTT 1 cut(s) 422
AclWI GGATC 1 cut(s) 561
AcsI RAATTY 3 cut(s) 170, 670, 1609
AcuI CTGAAG 2 cut(s) 27, 608
AfaI GTAC 6 cut(s) 62, 403, 637, 835, 1315, 1640
AfiI CCNNNNNNNGG 3 cut(s) 929, 1346, 1631
AflII CTTAAG 1 cut(s) 818
AhdI GACNNNNNGTC 1 cut(s) 1152
AjnI CCWGG 1 cut(s) 1548
AluBI AGCT 9 cut(s) 622, 725, 740, 937, 1118, 1138, 1162, 1452, 1504
AluI AGCT 9 cut(s) 622, 725, 740, 937, 1118, 1138, 1162, 1452, 1504
Alw21I GWGCWC 1 cut(s) 1506
Alw26I GTCTC 4 cut(s) 469, 737, 977, 1092
AlwI GGATC 1 cut(s) 561
Ama87I CYCGRG 1 cut(s) 1251
Aor13HI TCCGGA 1 cut(s) 844
AoxI GGCC 2 cut(s) 992, 1254
ApeKI GCWGC 1 cut(s) 934
ApoI RAATTY 3 cut(s) 170, 670, 1609
AspLEI GCGC 3 cut(s) 289, 492, 917
AspS9I GGNCC 2 cut(s) 992, 1254
AsuHPI GGTGA 2 cut(s) 12, 116
AvaI CYCGRG 1 cut(s) 1251
AxyI CCTNAGG 1 cut(s) 347
BaeI ACNNNNGTAYC 2 cut(s) 1166, 1199
BanII GRGCYC 1 cut(s) 1506
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 1506
BbvI GCAGC 1 cut(s) 946
BccI CCATC 5 cut(s) 34, 501, 1068, 1198, 1622
BciT130I CCWGG 1 cut(s) 1550
BcoDI GTCTC 4 cut(s) 469, 737, 977, 1092
BfaI CTAG 3 cut(s) 600, 722, 1308
BfmI CTRYAG 1 cut(s) 1519
BfrI CTTAAG 1 cut(s) 818
BglII AGATCT 1 cut(s) 1037
BisI GCNGC 1 cut(s) 935
BlsI GCNGC 1 cut(s) 936
BmcAI AGTACT 1 cut(s) 62
Bme1390I CCNGG 1 cut(s) 1550
BmeRI GACNNNNNGTC 1 cut(s) 1152
BmeT110I CYCGRG 1 cut(s) 1251
BmgT120I GGNCC 2 cut(s) 992, 1254
BmrFI CCNGG 1 cut(s) 1550
BmrI ACTGGG 1 cut(s) 938
BmsI GCATC 4 cut(s) 516, 670, 1208, 1348
BmuI ACTGGG 1 cut(s) 938
BpiI GAAGAC 1 cut(s) 51
BpmI CTGGAG 2 cut(s) 332, 770
BpuEI CTTGAG 2 cut(s) 1226, 1609
BsaBI GATNNNNATC 1 cut(s) 1042
BsaI GGTCTC 2 cut(s) 737, 1092
BsaJI CCNNGG 1 cut(s) 1548
BsaWI WCCGGW 1 cut(s) 844
Bsc4I CCNNNNNNNGG 3 cut(s) 929, 1346, 1631
Bse1I ACTGG 1 cut(s) 933
Bse21I CCTNAGG 1 cut(s) 347
Bse8I GATNNNNATC 1 cut(s) 1042
BseAI TCCGGA 1 cut(s) 844
BseBI CCWGG 1 cut(s) 1550
BseDI CCNNGG 1 cut(s) 1548
BseGI GGATG 4 cut(s) 193, 685, 1079, 1190
BseJI GATNNNNATC 1 cut(s) 1042
BseLI CCNNNNNNNGG 3 cut(s) 929, 1346, 1631
BseMII CTCAG 8 cut(s) 257, 467, 666, 732, 826, 987, 1170, 1594
BseNI ACTGG 1 cut(s) 933
BseRI GAGGAG 1 cut(s) 316
BseXI GCAGC 1 cut(s) 946
BsgI GTGCAG 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 289
BshFI GGCC 2 cut(s) 994, 1256
BsiHKAI GWGCWC 1 cut(s) 1506
BsiHKCI CYCGRG 1 cut(s) 1251
BsiSI CCGG 1 cut(s) 845
BslFI GGGAC 2 cut(s) 1140, 1561
BslI CCNNNNNNNGG 3 cut(s) 929, 1346, 1631
BsmAI GTCTC 4 cut(s) 469, 737, 977, 1092
BsmBI CGTCTC 1 cut(s) 977
BsmFI GGGAC 2 cut(s) 1140, 1561
BsmI GAATGC 2 cut(s) 97, 1421
BsnI GGCC 2 cut(s) 994, 1256
Bso31I GGTCTC 2 cut(s) 737, 1092
BsoBI CYCGRG 1 cut(s) 1251
Bsp1286I GDGCHC 1 cut(s) 1506
Bsp13I TCCGGA 1 cut(s) 844
Bsp143I GATC 4 cut(s) 462, 553, 859, 1037
BspACI CCGC 2 cut(s) 1311, 1475
BspANI GGCC 2 cut(s) 994, 1256
BspCNI CTCAG 8 cut(s) 256, 466, 667, 733, 825, 988, 1171, 1593
BspEI TCCGGA 1 cut(s) 844
BspFNI CGCG 1 cut(s) 289
BspPI GGATC 1 cut(s) 561
BspTI CTTAAG 1 cut(s) 818
BspTNI GGTCTC 2 cut(s) 737, 1092
BsrI ACTGG 1 cut(s) 933
BssECI CCNNGG 1 cut(s) 1548
BssMI GATC 4 cut(s) 462, 553, 859, 1037
Bst2UI CCWGG 1 cut(s) 1550
Bst4CI ACNGT 5 cut(s) 371, 1349, 1388, 1520, 1638
Bst6I CTCTTC 2 cut(s) 891, 1293
BstAFI CTTAAG 1 cut(s) 818
BstC8I GCNNGC 1 cut(s) 1223
BstDEI CTNAG 9 cut(s) 243, 347, 453, 675, 741, 812, 996, 1179, 1580
BstENI CCTNNNNNAGG 1 cut(s) 1629
BstF5I GGATG 4 cut(s) 193, 685, 1079, 1190
BstFNI CGCG 1 cut(s) 289
BstHHI GCGC 3 cut(s) 289, 492, 917
BstKTI GATC 4 cut(s) 465, 556, 862, 1040
BstMAI GTCTC 4 cut(s) 469, 737, 977, 1092
BstMBI GATC 4 cut(s) 462, 553, 859, 1037
BstMWI GCNNNNNNNGC 1 cut(s) 689
BstNI CCWGG 1 cut(s) 1550
BstSCI CCNGG 1 cut(s) 1548
BstSFI CTRYAG 1 cut(s) 1519
BstUI CGCG 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 946
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 2 cut(s) 553, 1037
BstXI CCANNNNNNTGG 1 cut(s) 1112
BstYI RGATCY 2 cut(s) 553, 1037
Bsu36I CCTNAGG 1 cut(s) 347
BsuRI GGCC 2 cut(s) 994, 1256
BtsCI GGATG 4 cut(s) 193, 685, 1079, 1190
BtsIMutI CAGTG 1 cut(s) 926
Cac8I GCNNGC 1 cut(s) 1223
CfoI GCGC 3 cut(s) 289, 492, 917
Cfr13I GGNCC 2 cut(s) 992, 1254
Csp6I GTAC 6 cut(s) 61, 402, 636, 834, 1314, 1639
CviAII CATG 3 cut(s) 127, 284, 732
CviQI GTAC 6 cut(s) 61, 402, 636, 834, 1314, 1639
DdeI CTNAG 9 cut(s) 243, 347, 453, 675, 741, 812, 996, 1179, 1580
DpnI GATC 4 cut(s) 464, 555, 861, 1039
DpnII GATC 4 cut(s) 462, 553, 859, 1037
DriI GACNNNNNGTC 1 cut(s) 1152
Eam1104I CTCTTC 2 cut(s) 891, 1293
Eam1105I GACNNNNNGTC 1 cut(s) 1152
EarI CTCTTC 2 cut(s) 891, 1293
Ecl136II GAGCTC 1 cut(s) 1504
Eco24I GRGCYC 1 cut(s) 1506
Eco31I GGTCTC 2 cut(s) 737, 1092
Eco53kI GAGCTC 1 cut(s) 1504
Eco57I CTGAAG 2 cut(s) 27, 608
Eco81I CCTNAGG 1 cut(s) 347
Eco88I CYCGRG 1 cut(s) 1251
EcoICRI GAGCTC 1 cut(s) 1504
EcoNI CCTNNNNNAGG 1 cut(s) 1629
EcoO109I RGGNCCY 1 cut(s) 992
EcoRI GAATTC 1 cut(s) 670
EcoRII CCWGG 1 cut(s) 1548
EcoT22I ATGCAT 1 cut(s) 1423
EcoT38I GRGCYC 1 cut(s) 1506
Esp3I CGTCTC 1 cut(s) 977
FaeI CATG 3 cut(s) 130, 287, 735
FalI AAGNNNNNCTT 2 cut(s) 1272, 1304
FaqI GGGAC 2 cut(s) 1140, 1561
FatI CATG 3 cut(s) 126, 283, 731
FauI CCCGC 1 cut(s) 1482
FauNDI CATATG 1 cut(s) 537
Fnu4HI GCNGC 1 cut(s) 935
FokI GGATG 4 cut(s) 200, 692, 1086, 1177
FriOI GRGCYC 1 cut(s) 1506
Fsp4HI GCNGC 1 cut(s) 935
FspBI CTAG 3 cut(s) 600, 722, 1308
GlaI GCGC 3 cut(s) 288, 491, 916
GluI GCNGC 1 cut(s) 935
GsuI CTGGAG 2 cut(s) 332, 770
HaeIII GGCC 2 cut(s) 994, 1256
HapII CCGG 1 cut(s) 845
HhaI GCGC 3 cut(s) 289, 492, 917
Hin1II CATG 3 cut(s) 130, 287, 735
Hin6I GCGC 3 cut(s) 287, 490, 915
HinP1I GCGC 3 cut(s) 287, 490, 915
HinfI GANTC 9 cut(s) 197, 223, 477, 752, 1267, 1457, 1525, 1532, 1553
HpaII CCGG 1 cut(s) 845
HphI GGTGA 2 cut(s) 12, 116
Hpy166II GTNNAC 3 cut(s) 638, 905, 1238
Hpy188I TCNGA 7 cut(s) 246, 627, 676, 1097, 1146, 1558, 1672
Hpy188III TCNNGA 8 cut(s) 165, 260, 311, 497, 845, 1295, 1529, 1625
Hpy8I GTNNAC 3 cut(s) 638, 905, 1238
HpyAV CCTTC 4 cut(s) 257, 544, 1103, 1281
HpyCH4III ACNGT 5 cut(s) 371, 1349, 1388, 1520, 1638
HpyCH4IV ACGT 2 cut(s) 48, 422
HpyCH4V TGCA 5 cut(s) 130, 295, 409, 596, 1421
HpyF10VI GCNNNNNNNGC 1 cut(s) 689
HpyF3I CTNAG 9 cut(s) 243, 347, 453, 675, 741, 812, 996, 1179, 1580
HpySE526I ACGT 2 cut(s) 48, 422
Hsp92II CATG 3 cut(s) 130, 287, 735
HspAI GCGC 3 cut(s) 287, 490, 915
Kpn2I TCCGGA 1 cut(s) 844
Kzo9I GATC 4 cut(s) 462, 553, 859, 1037
LmnI GCTCC 2 cut(s) 737, 1115
Lsp1109I GCAGC 1 cut(s) 946
LweI GCATC 4 cut(s) 516, 670, 1208, 1348
MaeI CTAG 3 cut(s) 600, 722, 1308
MaeII ACGT 2 cut(s) 48, 422
MaeIII GTNAC 5 cut(s) 18, 384, 569, 949, 1343
MalI GATC 4 cut(s) 464, 555, 861, 1039
MboI GATC 4 cut(s) 462, 553, 859, 1037
MboII GAAGA 7 cut(s) 56, 878, 908, 1048, 1178, 1310, 1450
MfeI CAATTG 1 cut(s) 436
MflI RGATCY 2 cut(s) 553, 1037
MhlI GDGCHC 1 cut(s) 1506
MlyI GAGTC 3 cut(s) 191, 217, 471
MmeI TCCRAC 3 cut(s) 31, 165, 1370
Mph1103I ATGCAT 1 cut(s) 1423
MroI TCCGGA 1 cut(s) 844
MslI CAYNNNNRTG 3 cut(s) 102, 498, 608
MspA1I CMGCKG 1 cut(s) 1477
MspCI CTTAAG 1 cut(s) 818
MspI CCGG 1 cut(s) 845
MspR9I CCNGG 1 cut(s) 1550
MunI CAATTG 1 cut(s) 436
Mva1269I GAATGC 2 cut(s) 97, 1421
MvaI CCWGG 1 cut(s) 1550
MvnI CGCG 1 cut(s) 289
MwoI GCNNNNNNNGC 1 cut(s) 689
NdeI CATATG 1 cut(s) 537
NdeII GATC 4 cut(s) 462, 553, 859, 1037
NlaIII CATG 3 cut(s) 130, 287, 735
NmuCI GTSAC 4 cut(s) 18, 569, 949, 1343
NsiI ATGCAT 1 cut(s) 1423
PctI GAATGC 2 cut(s) 97, 1421
PfeI GAWTC 6 cut(s) 752, 1267, 1457, 1525, 1532, 1553
PkrI GCNGC 1 cut(s) 936
PleI GAGTC 3 cut(s) 191, 217, 471
PpsI GAGTC 3 cut(s) 191, 217, 471
Psp124BI GAGCTC 1 cut(s) 1506
Psp1406I AACGTT 1 cut(s) 422
Psp6I CCWGG 1 cut(s) 1548
PspGI CCWGG 1 cut(s) 1548
PspPI GGNCC 2 cut(s) 992, 1254
PsuI RGATCY 2 cut(s) 553, 1037
RsaI GTAC 6 cut(s) 62, 403, 637, 835, 1315, 1640
RsaNI GTAC 6 cut(s) 61, 402, 636, 834, 1314, 1639
RseI CAYNNNNRTG 3 cut(s) 102, 498, 608
SacI GAGCTC 1 cut(s) 1506
SatI GCNGC 1 cut(s) 935
Sau3AI GATC 4 cut(s) 462, 553, 859, 1037
Sau96I GGNCC 2 cut(s) 992, 1254
ScaI AGTACT 1 cut(s) 62
SchI GAGTC 3 cut(s) 191, 217, 471
ScrFI CCNGG 1 cut(s) 1550
SduI GDGCHC 1 cut(s) 1506
SfaNI GCATC 4 cut(s) 516, 670, 1208, 1348
SfcI CTRYAG 1 cut(s) 1519
SmiMI CAYNNNNRTG 3 cut(s) 102, 498, 608
SmlI CTYRAG 3 cut(s) 818, 1205, 1588
SmoI CTYRAG 3 cut(s) 818, 1205, 1588
SsiI CCGC 2 cut(s) 1311, 1475
SspI AATATT 1 cut(s) 1596
SspMI CTAG 3 cut(s) 600, 722, 1308
SstI GAGCTC 1 cut(s) 1506
StyD4I CCNGG 1 cut(s) 1548
TaaI ACNGT 5 cut(s) 371, 1349, 1388, 1520, 1638
TaiI ACGT 2 cut(s) 51, 425
TaqI TCGA 3 cut(s) 80, 261, 894
TaqII GACCGA 1 cut(s) 761
TatI WGTACW 2 cut(s) 60, 401
TfiI GAWTC 6 cut(s) 752, 1267, 1457, 1525, 1532, 1553
TscAI CASTG 1 cut(s) 933
TseFI GTSAC 4 cut(s) 18, 569, 949, 1343
TseI GCWGC 1 cut(s) 934
Tsp45I GTSAC 4 cut(s) 18, 569, 949, 1343
TspDTI ATGAA 4 cut(s) 272, 459, 647, 990
TspGWI ACGGA 1 cut(s) 732
TspRI CASTG 1 cut(s) 933
Vha464I CTTAAG 1 cut(s) 818
XagI CCTNNNNNAGG 1 cut(s) 1629
XapI RAATTY 3 cut(s) 170, 670, 1609
XspI CTAG 3 cut(s) 600, 722, 1308
ZrmI AGTACT 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 1423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.