MD07G1187200.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0028851
Physical Location & Seq
Forward (+)
26816135 .. 26816641
507 bp
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UTR
Exon/CDS
Intron
MD07G1187200.v1.1.491

Sequence Viewer

Length: 507 bp
ATGCCACCAGACGACCTGAAGCTCAGCACTGATTCATGCGTGAAAATCGTCTTGCAGAACAGGAATCTGGGACTGGAAGAATTCAGGTTTCTCTTGAAAACTATTACCCATTCGGGCATTGGCGAGGAAACTTACTGCGCAAGAAACATCATCCAAGGCCGAGAAGAAACTGCAACCCTAGAAGATGAACTTGCGGAGATGGATGGCATCATCTTTGACGCGCTGGACAAGCTTTTTGCTTGGGCCACCTCAATTTCTCCATCGCAAATCGACATTCTTGTCGTCAATGTGTCGATGTTCTCCCCTGCACCCTCCCTATCGTCACGTATGGTGAATCGTTACAAGATGAGGGACGACATCAAGAGCTTCAACCTCTCAGGAATGGGCTGCAGTGCGACCCTCATTGCCATTGACGTTGTACAAAACTTGTTCAAGTCTCCCAAGAATGCGAATGCCATCGTCGTAAACACGGAATCGTTGGCTCCTCATTGGTATTGCGGCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

169

Amino Acids

18.6

Weight (kDa)

4.88

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 2 - 167 3.9e-60 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028851)

Species Orthologous Gene IDs
malus_domestica MD07G1187200.v1.1
pyrus_communis pycom03g10490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
Acc16I TGCGCA 1 cut(s) 139
AccII CGCG 1 cut(s) 221
AciI CCGC 2 cut(s) 194, 498
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 38
AfaI GTAC 1 cut(s) 420
AgsI TTSAA 3 cut(s) 97, 370, 433
AluBI AGCT 3 cut(s) 22, 232, 366
AluI AGCT 3 cut(s) 22, 232, 366
Alw26I GTCTC 1 cut(s) 441
AoxI GGCC 2 cut(s) 157, 243
ApeKI GCWGC 1 cut(s) 387
ApoI RAATTY 1 cut(s) 80
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
AspLEI GCGC 2 cut(s) 140, 223
AspS9I GGNCC 1 cut(s) 243
AsuHPI GGTGA 1 cut(s) 343
BbvI GCAGC 1 cut(s) 374
BccI CCATC 4 cut(s) 193, 197, 268, 464
BcoDI GTCTC 1 cut(s) 441
BfaI CTAG 1 cut(s) 179
BfmI CTRYAG 1 cut(s) 388
BisI GCNGC 2 cut(s) 388, 499
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 2 cut(s) 389, 500
BmgT120I GGNCC 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 483
BmsI GCATC 1 cut(s) 216
Bpu1102I GCTNAGC 1 cut(s) 23
BsaAI YACGTR 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 78
Bse3DI GCAATG 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 2 cut(s) 150, 208
BseMI GCAATG 1 cut(s) 402
BseMII CTCAG 2 cut(s) 37, 390
BseNI ACTGG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 474
BseXI GCAGC 1 cut(s) 374
BsgI GTGCAG 1 cut(s) 291
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 2 cut(s) 159, 245
BslFI GGGAC 2 cut(s) 84, 365
BsmAI GTCTC 1 cut(s) 441
BsmFI GGGAC 2 cut(s) 84, 365
BsmI GAATGC 2 cut(s) 451, 457
BsnI GGCC 2 cut(s) 159, 245
Bsp1407I TGTACA 1 cut(s) 418
Bsp1720I GCTNAGC 1 cut(s) 23
BspACI CCGC 2 cut(s) 194, 498
BspANI GGCC 2 cut(s) 159, 245
BspCNI CTCAG 2 cut(s) 36, 389
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 1 cut(s) 483
BspMAI CTGCAG 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 402
BsrGI TGTACA 1 cut(s) 418
BsrI ACTGG 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 154
BstAUI TGTACA 1 cut(s) 418
BstBAI YACGTR 1 cut(s) 326
BstDEI CTNAG 2 cut(s) 23, 376
BstF5I GGATG 2 cut(s) 150, 208
BstFNI CGCG 1 cut(s) 221
BstHHI GCGC 2 cut(s) 140, 223
BstMAI GTCTC 1 cut(s) 441
BstMWI GCNNNNNNNGC 1 cut(s) 229
BstSFI CTRYAG 1 cut(s) 388
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 374
BsuRI GGCC 2 cut(s) 159, 245
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 2 cut(s) 150, 208
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 2 cut(s) 27, 397
CfoI GCGC 2 cut(s) 140, 223
Cfr13I GGNCC 1 cut(s) 243
CseI GACGC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 419
CviAII CATG 1 cut(s) 36
CviJI RGCY 7 cut(s) 22, 159, 232, 245, 366, 387, 482
CviKI_1 RGCY 7 cut(s) 22, 159, 232, 245, 366, 387, 482
CviQI GTAC 1 cut(s) 419
DdeI CTNAG 2 cut(s) 23, 376
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 38
EcoRI GAATTC 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 1 cut(s) 39
FaiI YATR 2 cut(s) 37, 329
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 2 cut(s) 84, 365
FatI CATG 1 cut(s) 35
Fnu4HI GCNGC 2 cut(s) 388, 499
FokI GGATG 2 cut(s) 137, 215
Fsp4HI GCNGC 2 cut(s) 388, 499
FspBI CTAG 1 cut(s) 179
FspI TGCGCA 1 cut(s) 139
GlaI GCGC 2 cut(s) 139, 222
GluI GCNGC 2 cut(s) 388, 499
HaeIII GGCC 2 cut(s) 159, 245
HgaI GACGC 1 cut(s) 227
HhaI GCGC 2 cut(s) 140, 223
Hin1II CATG 1 cut(s) 39
Hin6I GCGC 2 cut(s) 138, 221
HinP1I GCGC 2 cut(s) 138, 221
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 4 cut(s) 32, 64, 334, 473
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 1 cut(s) 466
Hpy188III TCNNGA 3 cut(s) 94, 361, 378
Hpy8I GTNNAC 1 cut(s) 466
Hpy99I CGWCG 1 cut(s) 464
HpyCH4IV ACGT 2 cut(s) 325, 414
HpyCH4V TGCA 4 cut(s) 55, 173, 308, 390
HpyF10VI GCNNNNNNNGC 1 cut(s) 229
HpyF3I CTNAG 2 cut(s) 23, 376
HpySE526I ACGT 2 cut(s) 325, 414
Hsp92II CATG 1 cut(s) 39
HspAI GCGC 2 cut(s) 138, 221
LmnI GCTCC 1 cut(s) 487
LpnPI CCDG 9 cut(s) 21, 29, 46, 53, 59, 70, 209, 318, 363
Lsp1109I GCAGC 1 cut(s) 374
LweI GCATC 1 cut(s) 216
MaeI CTAG 1 cut(s) 179
MaeII ACGT 2 cut(s) 325, 414
MaeIII GTNAC 2 cut(s) 321, 338
MboII GAAGA 3 cut(s) 89, 176, 194
MluCI AATT 2 cut(s) 80, 252
MnlI CCTC 7 cut(s) 118, 259, 322, 342, 383, 410, 495
Mva1269I GAATGC 2 cut(s) 451, 457
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 1 cut(s) 229
NlaIII CATG 1 cut(s) 39
NlaIV GGNNCC 1 cut(s) 483
NmeAIII GCCGAG 1 cut(s) 185
NmuCI GTSAC 1 cut(s) 321
NsbI TGCGCA 1 cut(s) 139
PctI GAATGC 2 cut(s) 451, 457
PfeI GAWTC 4 cut(s) 32, 64, 334, 473
PkrI GCNGC 2 cut(s) 389, 500
Ppu21I YACGTR 1 cut(s) 326
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 1 cut(s) 243
PstI CTGCAG 1 cut(s) 392
RsaI GTAC 1 cut(s) 420
RsaNI GTAC 1 cut(s) 419
SatI GCNGC 2 cut(s) 388, 499
Sau96I GGNCC 1 cut(s) 243
SetI ASST 9 cut(s) 18, 24, 89, 234, 251, 328, 368, 375, 417
SfaNI GCATC 1 cut(s) 216
SfcI CTRYAG 1 cut(s) 388
Sse9I AATT 2 cut(s) 80, 252
SsiI CCGC 2 cut(s) 194, 498
SspMI CTAG 1 cut(s) 179
StyI CCWWGG 1 cut(s) 154
TaiI ACGT 2 cut(s) 328, 417
TaqI TCGA 2 cut(s) 270, 293
TasI AATT 2 cut(s) 80, 252
TatI WGTACW 1 cut(s) 418
TauI GCSGC 1 cut(s) 501
TfiI GAWTC 4 cut(s) 32, 64, 334, 473
TscAI CASTG 2 cut(s) 34, 397
TseFI GTSAC 1 cut(s) 321
TseI GCWGC 1 cut(s) 387
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 2 cut(s) 24, 201
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 2 cut(s) 34, 397
XapI RAATTY 1 cut(s) 80
XcmI CCANNNNNNNNNTGG 1 cut(s) 116
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.