MD07G1212500.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0024473
Physical Location & Seq
Reverse (-)
29052632 .. 29054148
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1212500.v1.1.491

Sequence Viewer

Length: 867 bp
ATGATCGCAACTCGTGCGCCAAGTCGAGGCGGCGACATTTCGACGGAGGAGCAACCGAACCATTCTGTCAAGCCCTACGACGCTAACGCTCACGCCGTGTTTATTCACCGCAGGGTGCGAAAGTTGTCTGAATTCTCAAGTTGGTGGTGGTGCAGATGGACTTCAATGGTTTTGATGGGTTTGGCCTTTCTGTCAATGCTGGCCAAGTTTGCCTTGCTAAACATGTTTCCAGAGTTAAGGGTGATAGAGAAAATAGTGAACGAAACAACCACAAACGGCTATCTCTTAGTTCGTTCCAATGGCGGATTGAACCAAATGAAGCTCGGGATAACTGATATGGTAGCAATAGCAAAACTTATGAATGCCACTCTTGTCCTCCCTTCTTTGGATCACAGATCCTTTTGGACCAACTCAAGTGACTTTAAAGACATTTTCGATTGGAAGAACTTCATGGAAGTTTTGAGAGACGATATCATGATTGTAAAGTCTCTCCCACTGGAGTTTGCAGACGTGAAGCCTTGCCTTAAGAATCCGGTGTCTTGGTCAAAGTCCAGTTACTATAGACTACAGATGGTAACATTGTTGAAGAAGCACAAGGTTGTCAAATTTACCCTCACAAATTCTAGACTTGCTAATAATGTTGGTAGCTCGATCCAGAGGCTCCGCTGCCGCGCCCTTTATAAGGCACTGAAATTCGCAACTGAAATCGAAGAGCTTGGGATGAATTTGGTTGACAGATTAAAGAACAATGGTAGCAAATACATTGCTCTACATCTAAGATATGAAAAGGACATGTTTTCTTTCAAAGGCTGCAGTCACAATCTCACCATACACGAAGATAAAGAAATCAAAGGGAAAAGAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

289

Amino Acids

33.19

Weight (kDa)

9.75

Isoelectric Point (pI)

31.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 94 - 287 2.2e-58 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024473)

Species Orthologous Gene IDs
malus_domestica MD07G1212500.v1.1
pyrus_communis pycom05g13880
rosa_multiflora Rmu_co8207126.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 681
AccII CGCG 1 cut(s) 672
AciI CCGC 5 cut(s) 30, 109, 303, 664, 670
AclWI GGATC 3 cut(s) 390, 396, 646
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 5 cut(s) 131, 605, 619, 692, 724
AdeI CACNNNGTG 1 cut(s) 97
AfiI CCNNNNNNNGG 3 cut(s) 26, 385, 682
AflII CTTAAG 1 cut(s) 524
AflIII ACRYGT 2 cut(s) 222, 792
AgsI TTSAA 4 cut(s) 165, 310, 586, 805
AjiI CACGTC 1 cut(s) 511
AluBI AGCT 3 cut(s) 322, 648, 715
AluI AGCT 3 cut(s) 322, 648, 715
Alw26I GTCTC 2 cut(s) 459, 492
AlwI GGATC 3 cut(s) 390, 396, 646
Ama87I CYCGRG 1 cut(s) 323
AoxI GGCC 2 cut(s) 183, 201
ApeKI GCWGC 2 cut(s) 666, 810
ApoI RAATTY 5 cut(s) 131, 605, 619, 692, 724
Asp700I GAANNNNTTC 1 cut(s) 446
AspLEI GCGC 2 cut(s) 19, 674
AspS9I GGNCC 1 cut(s) 405
AsuHPI GGTGA 3 cut(s) 98, 253, 817
AvaI CYCGRG 1 cut(s) 323
AvaII GGWCC 1 cut(s) 405
BalI TGGCCA 1 cut(s) 203
BauI CACGAG 1 cut(s) 12
BbvI GCAGC 2 cut(s) 653, 797
BccI CCATC 3 cut(s) 150, 169, 565
BceAI ACGGC 2 cut(s) 80, 292
BcoDI GTCTC 2 cut(s) 459, 492
BfaI CTAG 1 cut(s) 624
BfmI CTRYAG 3 cut(s) 559, 566, 811
BfrI CTTAAG 1 cut(s) 524
BisI GCNGC 4 cut(s) 31, 667, 670, 811
BlsI GCNGC 4 cut(s) 32, 668, 671, 812
Bme18I GGWCC 1 cut(s) 405
BmeT110I CYCGRG 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 511
BmgT120I GGNCC 1 cut(s) 405
BmiI GGNNCC 1 cut(s) 662
BpmI CTGGAG 1 cut(s) 518
BpuEI CTTGAG 2 cut(s) 121, 397
BsaWI WCCGGW 1 cut(s) 532
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 385, 682
Bse1I ACTGG 2 cut(s) 501, 552
Bse3DI GCAATG 1 cut(s) 762
BseGI GGATG 1 cut(s) 726
BseLI CCNNNNNNNGG 3 cut(s) 26, 385, 682
BseMI GCAATG 1 cut(s) 762
BseNI ACTGG 2 cut(s) 501, 552
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 2 cut(s) 653, 797
BsgI GTGCAG 1 cut(s) 172
Bsh1236I CGCG 1 cut(s) 672
BshFI GGCC 2 cut(s) 185, 203
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 1 cut(s) 533
BslI CCNNNNNNNGG 3 cut(s) 26, 385, 682
BsmAI GTCTC 2 cut(s) 459, 492
BsmBI CGTCTC 1 cut(s) 459
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 2 cut(s) 185, 203
BsoBI CYCGRG 1 cut(s) 323
Bsp143I GATC 4 cut(s) 3, 388, 395, 651
BspACI CCGC 5 cut(s) 30, 109, 303, 664, 670
BspANI GGCC 2 cut(s) 185, 203
BspFNI CGCG 1 cut(s) 672
BspHI TCATGA 1 cut(s) 474
BspLI GGNNCC 1 cut(s) 662
BspMAI CTGCAG 1 cut(s) 815
BspPI GGATC 3 cut(s) 390, 396, 646
BspQI GCTCTTC 1 cut(s) 705
BspTI CTTAAG 1 cut(s) 524
BsrDI GCAATG 1 cut(s) 762
BsrI ACTGG 2 cut(s) 501, 552
BssMI GATC 4 cut(s) 3, 388, 395, 651
BssSI CACGAG 1 cut(s) 12
Bst2BI CACGAG 1 cut(s) 12
Bst6I CTCTTC 1 cut(s) 705
BstAFI CTTAAG 1 cut(s) 524
BstAPI GCANNNNNTGC 1 cut(s) 14
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 2 cut(s) 286, 776
BstENI CCTNNNNNAGG 1 cut(s) 680
BstF5I GGATG 1 cut(s) 726
BstFNI CGCG 1 cut(s) 672
BstHHI GCGC 2 cut(s) 19, 674
BstKTI GATC 4 cut(s) 6, 391, 398, 654
BstMAI GTCTC 2 cut(s) 459, 492
BstMBI GATC 4 cut(s) 3, 388, 395, 651
BstMWI GCNNNNNNNGC 2 cut(s) 14, 209
BstNSI RCATGY 2 cut(s) 226, 796
BstSFI CTRYAG 3 cut(s) 559, 566, 811
BstUI CGCG 1 cut(s) 672
BstV1I GCAGC 2 cut(s) 653, 797
BstX2I RGATCY 1 cut(s) 395
BstYI RGATCY 1 cut(s) 395
BsuRI GGCC 2 cut(s) 185, 203
BtrI CACGTC 1 cut(s) 511
BtsCI GGATG 1 cut(s) 726
BtsIMutI CAGTG 2 cut(s) 494, 686
Cac8I GCNNGC 1 cut(s) 201
CciI TCATGA 1 cut(s) 474
CfoI GCGC 2 cut(s) 19, 674
Cfr13I GGNCC 1 cut(s) 405
CseI GACGC 1 cut(s) 89
CviAII CATG 4 cut(s) 223, 451, 475, 793
DdeI CTNAG 2 cut(s) 286, 776
DpnI GATC 4 cut(s) 5, 390, 397, 653
DpnII GATC 4 cut(s) 3, 388, 395, 651
DraI TTTAAA 1 cut(s) 424
DraIII CACNNNGTG 1 cut(s) 97
EaeI YGGCCR 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 705
EarI CTCTTC 1 cut(s) 705
EciI GGCGGA 1 cut(s) 318
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 405
Eco88I CYCGRG 1 cut(s) 323
EcoNI CCTNNNNNAGG 1 cut(s) 680
EcoRI GAATTC 1 cut(s) 131
EcoRV GATATC 1 cut(s) 472
Esp3I CGTCTC 1 cut(s) 459
FaeI CATG 4 cut(s) 226, 454, 478, 796
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 4 cut(s) 222, 450, 474, 792
Fnu4HI GCNGC 4 cut(s) 31, 667, 670, 811
FokI GGATG 1 cut(s) 733
Fsp4HI GCNGC 4 cut(s) 31, 667, 670, 811
FspBI CTAG 1 cut(s) 624
GlaI GCGC 2 cut(s) 18, 673
GluI GCNGC 4 cut(s) 31, 667, 670, 811
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 2 cut(s) 185, 203
HapII CCGG 1 cut(s) 533
HgaI GACGC 1 cut(s) 89
HhaI GCGC 2 cut(s) 19, 674
Hin1II CATG 4 cut(s) 226, 454, 478, 796
Hin6I GCGC 2 cut(s) 17, 672
HinP1I GCGC 2 cut(s) 17, 672
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HinfI GANTC 1 cut(s) 529
HpaII CCGG 1 cut(s) 533
HphI GGTGA 3 cut(s) 98, 253, 817
Hpy166II GTNNAC 2 cut(s) 259, 733
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 5 cut(s) 230, 325, 475, 624, 655
Hpy8I GTNNAC 2 cut(s) 259, 733
Hpy99I CGWCG 2 cut(s) 46, 83
HpyAV CCTTC 1 cut(s) 390
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 3 cut(s) 153, 506, 813
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 209
HpyF3I CTNAG 2 cut(s) 286, 776
HpySE526I ACGT 1 cut(s) 510
Hsp92II CATG 4 cut(s) 226, 454, 478, 796
HspAI GCGC 2 cut(s) 17, 672
Kzo9I GATC 4 cut(s) 3, 388, 395, 651
LguI GCTCTTC 1 cut(s) 705
LmnI GCTCC 2 cut(s) 49, 666
LpnPI CCDG 7 cut(s) 97, 185, 243, 482, 546, 565, 668
Lsp1109I GCAGC 2 cut(s) 653, 797
MaeI CTAG 1 cut(s) 624
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 4 cut(s) 416, 554, 574, 815
MalI GATC 4 cut(s) 5, 390, 397, 653
MboI GATC 4 cut(s) 3, 388, 395, 651
MboII GAAGA 4 cut(s) 454, 598, 722, 848
MflI RGATCY 1 cut(s) 395
MlsI TGGCCA 1 cut(s) 203
MluCI AATT 5 cut(s) 131, 605, 619, 692, 724
MluNI TGGCCA 1 cut(s) 203
MnlI CCTC 6 cut(s) 20, 40, 386, 623, 651, 854
Mox20I TGGCCA 1 cut(s) 203
MroXI GAANNNNTTC 1 cut(s) 446
MscI TGGCCA 1 cut(s) 203
MseI TTAA 4 cut(s) 236, 423, 525, 740
Msp20I TGGCCA 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 666
MspCI CTTAAG 1 cut(s) 524
MspI CCGG 1 cut(s) 533
Mva1269I GAATGC 1 cut(s) 367
MvnI CGCG 1 cut(s) 672
MwoI GCNNNNNNNGC 2 cut(s) 14, 209
NdeII GATC 4 cut(s) 3, 388, 395, 651
NlaIII CATG 4 cut(s) 226, 454, 478, 796
NlaIV GGNNCC 1 cut(s) 662
NmuCI GTSAC 2 cut(s) 416, 815
NspI RCATGY 2 cut(s) 226, 796
PagI TCATGA 1 cut(s) 474
PciI ACATGT 2 cut(s) 222, 792
PciSI GCTCTTC 1 cut(s) 705
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 446
PfeI GAWTC 1 cut(s) 529
PkrI GCNGC 4 cut(s) 32, 668, 671, 812
PscI ACATGT 2 cut(s) 222, 792
PsiI TTATAA 1 cut(s) 681
PspN4I GGNNCC 1 cut(s) 662
PspPI GGNCC 1 cut(s) 405
PstI CTGCAG 1 cut(s) 815
PsuI RGATCY 1 cut(s) 395
SapI GCTCTTC 1 cut(s) 705
SaqAI TTAA 4 cut(s) 236, 423, 525, 740
SatI GCNGC 4 cut(s) 31, 667, 670, 811
Sau3AI GATC 4 cut(s) 3, 388, 395, 651
Sau96I GGNCC 1 cut(s) 405
SetI ASST 5 cut(s) 324, 513, 600, 650, 717
SfcI CTRYAG 3 cut(s) 559, 566, 811
SinI GGWCC 1 cut(s) 405
SmlI CTYRAG 3 cut(s) 136, 412, 524
SmoI CTYRAG 3 cut(s) 136, 412, 524
Sse9I AATT 5 cut(s) 131, 605, 619, 692, 724
SsiI CCGC 5 cut(s) 30, 109, 303, 664, 670
SspMI CTAG 1 cut(s) 624
TaiI ACGT 1 cut(s) 513
TaqI TCGA 5 cut(s) 25, 41, 435, 650, 708
TasI AATT 5 cut(s) 131, 605, 619, 692, 724
TauI GCSGC 2 cut(s) 33, 672
TfiI GAWTC 1 cut(s) 529
Tru1I TTAA 4 cut(s) 236, 423, 525, 740
Tru9I TTAA 4 cut(s) 236, 423, 525, 740
TscAI CASTG 2 cut(s) 501, 693
TseFI GTSAC 2 cut(s) 416, 815
TseI GCWGC 2 cut(s) 666, 810
Tsp45I GTSAC 2 cut(s) 416, 815
TspDTI ATGAA 5 cut(s) 332, 374, 439, 737, 798
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 2 cut(s) 501, 693
Vha464I CTTAAG 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 405
XagI CCTNNNNNAGG 1 cut(s) 680
XapI RAATTY 5 cut(s) 131, 605, 619, 692, 724
XbaI TCTAGA 1 cut(s) 623
XceI RCATGY 2 cut(s) 226, 796
XmnI GAANNNNTTC 1 cut(s) 446
XspI CTAG 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.