Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0013066

Physical Location & Seq

Genomic Coordinates Forward (+)

29832307 .. 29837736

Gene Structure 5430 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1220800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1440 bp

ATGGATTTCCTCTTCTCATTTTTCTTGGTTTTCTGTCTCCTTTCAACCCTAGTTTTTATTTTTCTCAGAAAGTCTAGGTGCCGGAGGCTGAGGCTGCCTCCGGGGAACCTAGGGTTACCATTTATAGGGGAGACTCTGCAGCTTATCTCAGCCTACAAGACTGAGAACCCGGAACCGTTCATCGACGAGCGCGTGAACCGGTTCGGACCCTTGTTCACAACTCACGTGTTCGGAGAGCCGACCGTGTTCTCCACCGACCCGGAGACCAATCGTTTCATCTTGCAGAACGAAGGGAAGCTGTTCGAGTGCAGCTACCCGGGCTCCATAACCAACCTTATGGGGAAGCACTCTTTGCTGCTGATGAAGGGCAGCCTCCATAAACGGATGCATTCTCTGACCATGAGCTTTGCAAACTCTTCGATCATTAGGGACCACCTTTTGGACGACATAGACCGGTTGATCCGGCTCAACATGGATTCCTGGGCCGACCGGATCTTTCTCATGGAGGAGGCCAAAAAGATAACTTTTGAACTAGCAGTGAAGCAACTCATGAGCTTTGATCCAAATGAATGGACCGAGAGTCTAAGAAAGGAGTACGTCCTACTAATCGAAGGCTTCTTCTCCGTTCCTTTGCCTCTAGTTTCCACCACCTACCGCCGGGCCATTACCGCTAGGACAAAGGTGGCGGAAGCGTTGAGCTTGATAGTGAGGCAGAGGAGGGAGGAGAGCGAAGCCGGGAAGAGAAAAGAGGACATGTTAGGCGCACTTTTGGGCGGAGACACCGCATTCTCAGATGAGGAGATTGTGGATTTCTTGCTTGCTTTGCTGGTGGCTGGCTACGAAACCACTTCAACAATCATGACACTTGCCGTCAAGTTCTTAACGGAGACACCTCTGGCCCATGCTCAACTCAAGGAAGAACATGAGCAAATTAGGGCGAAAAAGAAGTCGTCAGAGCCTCTAGAATGGAGTGATTATAAGGCAATGCCATTCACTCAATGTGTTGTCAACGAGACCTTGCGAGTTGCAAACATAATTAGCGGGGTATTTAGGCGGGCAATGACGGACGTTCACATTAAGGGATACACAATCCCCAAAGGATGGAAGGCATTTGCATCGTTTCGTGCCGTACATCTAGACCAAGATCATTTCAAGGACGCTCGAACTTTTAATCCATGGAGGTGGCAGAACAAATCTGGACCGACGACAAGCCCATCAAATGTGTTCACACCATTTGGAGGAGGGCCAAGGTTCTGCCCCGGCTCTGAGCTTGCTAGAGTAGAAATCTCCGTCTTCCTTCATCACTTGGTCACTCGTTTCAGTTGGGTTCCTGCCGAGGAAGATAAGCTGGTGTTTTTTCCGACGACGAGGACACAGAAGAGGTACCCGATCAATGTGCAGCGGAGGAACAATGTGTCATATGGGCAATGTAAAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004497 GO:0006629 GO:0006694 GO:0007275 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009555 GO:0009628 GO:0009653 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0009987 GO:0010208 GO:0010224 GO:0010268 GO:0010584 GO:0010817 GO:0010927 GO:0016043 GO:0016125 GO:0016128 GO:0016129 GO:0016131 GO:0016132 GO:0016491 GO:0022414 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0042445 GO:0042446 GO:0042592 GO:0043062 GO:0044085 GO:0044238 GO:0045229 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048518 GO:0048580 GO:0048582 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0055088 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0080132 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:1905392 GO:1905393 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko00905 ko01100 ko01110 map00905 map01100 map01110

Pfam Domains

Protein Families

p450

Protein Analysis

480

Amino Acids

55.0

Weight (kDa)

8.97

Isoelectric Point (pI)

43.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	33 - 450	1.3e-62	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013066)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05690 AT5G05690
fragaria_vesca	FvH4_7g24370
malus_domestica	MD01G1152100.v1.1 MD07G1220800.v1.1
prunus_persica	Prupe.2G249100_v2.0.a1 Prupe.2G249100_v2.0.a1
pyrus_communis	pycom07g19970
rosa_chinensis	RchiOBHm_Chr1g0370071
rosa_laevigata	RLG00000027077
rosa_multiflora	Rmu_sc0005056.1_g000010
rosa_roxburghii	Rroxscaffold_4G00286850 Rroxscaffold_4G00287180
rosa_rugosa	Rorug01G0353600
rosa_samantha	Rh1AG362300 Rh1BG325500 Rh1CG338800
rosa_wichuraiana	Rw1G031880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	978
Acc65I	GGTACC	1 cut(s)	1383
AccB1I	GGYRCC	2 cut(s)	78, 1383
AccB7I	CCANNNNNTGG	2 cut(s)	439, 1101
AccII	CGCG	1 cut(s)	192
AciI	CCGC	8 cut(s)	655, 669, 686, 774, 783, 1041, 1054, 1402
AclWI	GGATC	3 cut(s)	454, 500, 554
AcvI	CACGTG	1 cut(s)	226
AfaI	GTAC	3 cut(s)	596, 1131, 1385
AfiI	CCNNNNNNNGG	5 cut(s)	125, 439, 657, 1101, 1238
AflIII	ACRYGT	2 cut(s)	225, 753
AgeI	ACCGGT	2 cut(s)	198, 453
AgsI	TTSAA	4 cut(s)	45, 530, 852, 1153
AhdI	GACNNNNNGTC	1 cut(s)	579
AjnI	CCWGG	1 cut(s)	479
AloI	GAACNNNNNNTCC	2 cut(s)	1156, 1188
AluBI	AGCT	8 cut(s)	142, 298, 312, 405, 555, 699, 1270, 1348
AluI	AGCT	8 cut(s)	142, 298, 312, 405, 555, 699, 1270, 1348
Alw26I	GTCTC	6 cut(s)	41, 125, 257, 771, 881, 1007
AlwI	GGATC	3 cut(s)	454, 500, 554
Ama87I	CYCGRG	1 cut(s)	316
AoxI	GGCC	5 cut(s)	483, 510, 660, 897, 1244
ApeKI	GCWGC	6 cut(s)	94, 139, 309, 355, 369, 1399
ArsI	GACNNNNNNTTYG	2 cut(s)	932, 964
AsiGI	ACCGGT	2 cut(s)	198, 453
Asp700I	GAANNNNTTC	2 cut(s)	200, 299
Asp718I	GGTACC	1 cut(s)	1383
AspA2I	CCTAGG	1 cut(s)	109
AspLEI	GCGC	2 cut(s)	192, 764
AspS9I	GGNCC	8 cut(s)	206, 430, 483, 573, 660, 898, 1199, 1244
AsuC2I	CCSGG	8 cut(s)	102, 170, 260, 317, 318, 659, 736, 1260
AvaI	CYCGRG	1 cut(s)	316
AvaII	GGWCC	4 cut(s)	206, 430, 573, 1199
AvrII	CCTAGG	1 cut(s)	109
BanI	GGYRCC	2 cut(s)	78, 1383
BanII	GRGCYC	1 cut(s)	323
BbrPI	CACGTG	1 cut(s)	226
BbsI	GAAGAC	1 cut(s)	1285
BbvCI	CCTCAGC	1 cut(s)	89
BbvI	GCAGC	6 cut(s)	81, 151, 321, 342, 381, 1411
BccI	CCATC	2 cut(s)	1095, 1222
BceAI	ACGGC	2 cut(s)	854, 1112
BcgI	CGANNNNNNTGC	6 cut(s)	399, 433, 1098, 1132, 1378, 1412
BciT130I	CCWGG	1 cut(s)	481
BciVI	GTATCC	1 cut(s)	1076
BcnI	CCSGG	8 cut(s)	102, 170, 260, 317, 318, 659, 736, 1260
BcoDI	GTCTC	6 cut(s)	41, 125, 257, 771, 881, 1007
BfaI	CTAG	9 cut(s)	50, 75, 110, 533, 638, 672, 962, 1136, 1275
BfmI	CTRYAG	1 cut(s)	137
BfuI	GTATCC	1 cut(s)	1076
BisI	GCNGC	6 cut(s)	95, 140, 310, 356, 370, 1400
BlnI	CCTAGG	1 cut(s)	109
BlsI	GCNGC	6 cut(s)	96, 141, 311, 357, 371, 1401
Bme1390I	CCNGG	9 cut(s)	102, 170, 260, 317, 318, 481, 659, 736, 1260
Bme18I	GGWCC	4 cut(s)	206, 430, 573, 1199
BmeRI	GACNNNNNGTC	1 cut(s)	579
BmeT110I	CYCGRG	1 cut(s)	316
BmgT120I	GGNCC	8 cut(s)	206, 430, 483, 573, 660, 898, 1199, 1244
BmiI	GGNNCC	8 cut(s)	80, 107, 174, 208, 322, 431, 1329, 1385
BmrFI	CCNGG	9 cut(s)	102, 170, 260, 317, 318, 481, 659, 736, 1260
BmsI	GCATC	2 cut(s)	375, 1124
BpiI	GAAGAC	1 cut(s)	1285
BplI	GAGNNNNNCTC	2 cut(s)	82, 114
Bpu10I	CCTNAGC	1 cut(s)	89
BpuEI	CTTGAG	1 cut(s)	896
BpuMI	CCSGG	8 cut(s)	102, 170, 260, 317, 318, 659, 736, 1260
BsaAI	YACGTR	1 cut(s)	226
BsaI	GGTCTC	2 cut(s)	257, 1007
BsaJI	CCNNGG	8 cut(s)	101, 109, 316, 480, 1175, 1247, 1258, 1335
BsaWI	WCCGGW	3 cut(s)	198, 453, 489
BsaXI	ACNNNNNCTCC	2 cut(s)	305, 335
Bsc4I	CCNNNNNNNGG	5 cut(s)	125, 439, 657, 1101, 1238
Bse118I	RCCGGY	2 cut(s)	198, 453
Bse3DI	GCAATG	3 cut(s)	990, 1065, 1433
BseBI	CCWGG	1 cut(s)	481
BseDI	CCNNGG	8 cut(s)	101, 109, 316, 480, 1175, 1247, 1258, 1335
BseGI	GGATG	2 cut(s)	390, 1106
BseLI	CCNNNNNNNGG	5 cut(s)	125, 439, 657, 1101, 1238
BseMI	GCAATG	3 cut(s)	990, 1065, 1433
BseMII	CTCAG	6 cut(s)	79, 80, 153, 162, 804, 1257
BseRI	GAGGAG	5 cut(s)	521, 730, 737, 812, 1254
BseXI	GCAGC	6 cut(s)	81, 151, 321, 342, 381, 1411
BsgI	GTGCAG	2 cut(s)	328, 1418
Bsh1236I	CGCG	1 cut(s)	192
Bsh1285I	CGRYCG	2 cut(s)	243, 490
BshFI	GGCC	5 cut(s)	485, 512, 662, 899, 1246
BshNI	GGYRCC	2 cut(s)	78, 1383
BshTI	ACCGGT	2 cut(s)	198, 453
BsiEI	CGRYCG	2 cut(s)	243, 490
BsiHKCI	CYCGRG	1 cut(s)	316
BslFI	GGGAC	1 cut(s)	443
BslI	CCNNNNNNNGG	5 cut(s)	125, 439, 657, 1101, 1238
BsmAI	GTCTC	6 cut(s)	41, 125, 257, 771, 881, 1007
BsmFI	GGGAC	1 cut(s)	443
BsmI	GAATGC	2 cut(s)	388, 785
BsnI	GGCC	5 cut(s)	485, 512, 662, 899, 1246
Bso31I	GGTCTC	2 cut(s)	257, 1007
BsoBI	CYCGRG	1 cut(s)	316
Bsp1286I	GDGCHC	1 cut(s)	323
Bsp143I	GATC	6 cut(s)	420, 459, 492, 559, 1144, 1389
Bsp19I	CCATGG	1 cut(s)	1175
BspACI	CCGC	8 cut(s)	655, 669, 686, 774, 783, 1041, 1054, 1402
BspANI	GGCC	5 cut(s)	485, 512, 662, 899, 1246
BspCNI	CTCAG	6 cut(s)	78, 81, 154, 161, 803, 1258
BspFNI	CGCG	1 cut(s)	192
BspHI	TCATGA	2 cut(s)	549, 858
BspLI	GGNNCC	8 cut(s)	80, 107, 174, 208, 322, 431, 1329, 1385
BspMAI	CTGCAG	1 cut(s)	141
BspPI	GGATC	3 cut(s)	454, 500, 554
BspT107I	GGYRCC	2 cut(s)	78, 1383
BspTNI	GGTCTC	2 cut(s)	257, 1007
BsrDI	GCAATG	3 cut(s)	990, 1065, 1433
BsrFI	RCCGGY	2 cut(s)	198, 453
BssAI	RCCGGY	2 cut(s)	198, 453
BssECI	CCNNGG	8 cut(s)	101, 109, 316, 480, 1175, 1247, 1258, 1335
BssMI	GATC	6 cut(s)	420, 459, 492, 559, 1144, 1389
BssT1I	CCWWGG	3 cut(s)	109, 1175, 1247
Bst2UI	CCWGG	1 cut(s)	481
Bst4CI	ACNGT	2 cut(s)	177, 244
Bst6I	CTCTTC	4 cut(s)	17, 421, 734, 1373
BstAPI	GCANNNNNTGC	1 cut(s)	352
BstBAI	YACGTR	1 cut(s)	226
BstC8I	GCNNGC	4 cut(s)	819, 835, 1056, 1272
BstDEI	CTNAG	7 cut(s)	65, 89, 148, 162, 584, 790, 1266
BstDSI	CCRYGG	1 cut(s)	1175
BstEII	GGTNACC	1 cut(s)	114
BstF5I	GGATG	2 cut(s)	390, 1106
BstFNI	CGCG	1 cut(s)	192
BstHHI	GCGC	2 cut(s)	192, 764
BstKTI	GATC	6 cut(s)	423, 462, 495, 562, 1147, 1392
BstMAI	GTCTC	6 cut(s)	41, 125, 257, 771, 881, 1007
BstMBI	GATC	6 cut(s)	420, 459, 492, 559, 1144, 1389
BstMCI	CGRYCG	2 cut(s)	243, 490
BstMWI	GCNNNNNNNGC	5 cut(s)	94, 318, 352, 668, 823
BstNI	CCWGG	1 cut(s)	481
BstNSI	RCATGY	1 cut(s)	757
BstPI	GGTNACC	1 cut(s)	114
BstSCI	CCNGG	9 cut(s)	100, 168, 258, 315, 316, 479, 657, 734, 1258
BstSFI	CTRYAG	1 cut(s)	137
BstUI	CGCG	1 cut(s)	192
BstV1I	GCAGC	6 cut(s)	81, 151, 321, 342, 381, 1411
BstV2I	GAAGAC	1 cut(s)	1285
BstX2I	RGATCY	1 cut(s)	492
BstXI	CCANNNNNNTGG	3 cut(s)	337, 570, 1182
BstYI	RGATCY	1 cut(s)	492
BsuI	GTATCC	1 cut(s)	1076
BsuRI	GGCC	5 cut(s)	485, 512, 662, 899, 1246
BtgI	CCRYGG	1 cut(s)	1175
BtsCI	GGATG	2 cut(s)	390, 1106
BtsI	GCAGTG	1 cut(s)	543
BtsIMutI	CAGTG	1 cut(s)	543
Cac8I	GCNNGC	4 cut(s)	819, 835, 1056, 1272
CciI	TCATGA	2 cut(s)	549, 858
CfoI	GCGC	2 cut(s)	192, 764
Cfr10I	RCCGGY	2 cut(s)	198, 453
Cfr13I	GGNCC	8 cut(s)	206, 430, 483, 573, 660, 898, 1199, 1244
Cfr9I	CCCGGG	1 cut(s)	316
CseI	GACGC	1 cut(s)	1166
Csp6I	GTAC	3 cut(s)	595, 1130, 1384
CspAI	ACCGGT	2 cut(s)	198, 453
CviAII	CATG	9 cut(s)	400, 472, 502, 550, 754, 859, 902, 923, 1176
CviQI	GTAC	3 cut(s)	595, 1130, 1384
DdeI	CTNAG	7 cut(s)	65, 89, 148, 162, 584, 790, 1266
DpnI	GATC	6 cut(s)	422, 461, 494, 561, 1146, 1391
DpnII	GATC	6 cut(s)	420, 459, 492, 559, 1144, 1389
DriI	GACNNNNNGTC	1 cut(s)	579
Eam1104I	CTCTTC	4 cut(s)	17, 421, 734, 1373
Eam1105I	GACNNNNNGTC	1 cut(s)	579
EarI	CTCTTC	4 cut(s)	17, 421, 734, 1373
EciI	GGCGGA	2 cut(s)	701, 789
Eco130I	CCWWGG	3 cut(s)	109, 1175, 1247
Eco24I	GRGCYC	1 cut(s)	323
Eco31I	GGTCTC	2 cut(s)	257, 1007
Eco47I	GGWCC	4 cut(s)	206, 430, 573, 1199
Eco72I	CACGTG	1 cut(s)	226
Eco88I	CYCGRG	1 cut(s)	316
Eco91I	GGTNACC	1 cut(s)	114
EcoO65I	GGTNACC	1 cut(s)	114
EcoRII	CCWGG	1 cut(s)	479
EcoT14I	CCWWGG	3 cut(s)	109, 1175, 1247
EcoT22I	ATGCAT	1 cut(s)	390
EcoT38I	GRGCYC	1 cut(s)	323
ErhI	CCWWGG	3 cut(s)	109, 1175, 1247
FaeI	CATG	9 cut(s)	403, 475, 505, 553, 757, 862, 905, 926, 1179
FaqI	GGGAC	1 cut(s)	443
FatI	CATG	9 cut(s)	399, 471, 501, 549, 753, 858, 901, 922, 1175
FauI	CCCGC	2 cut(s)	1034, 1047
FauNDI	CATATG	1 cut(s)	1420
Fnu4HI	GCNGC	6 cut(s)	95, 140, 310, 356, 370, 1400
FokI	GGATG	2 cut(s)	397, 1113
FriOI	GRGCYC	1 cut(s)	323
Fsp4HI	GCNGC	6 cut(s)	95, 140, 310, 356, 370, 1400
FspBI	CTAG	9 cut(s)	50, 75, 110, 533, 638, 672, 962, 1136, 1275
GlaI	GCGC	2 cut(s)	191, 763
GluI	GCNGC	6 cut(s)	95, 140, 310, 356, 370, 1400
HaeIII	GGCC	5 cut(s)	485, 512, 662, 899, 1246
HgaI	GACGC	1 cut(s)	1166
HhaI	GCGC	2 cut(s)	192, 764
Hin1II	CATG	9 cut(s)	403, 475, 505, 553, 757, 862, 905, 926, 1179
Hin6I	GCGC	2 cut(s)	190, 762
HinP1I	GCGC	2 cut(s)	190, 762
HincII	GTYRAC	1 cut(s)	1009
HindII	GTYRAC	1 cut(s)	1009
HinfI	GANTC	3 cut(s)	133, 476, 580
Hpy166II	GTNNAC	5 cut(s)	196, 216, 1009, 1072, 1227
Hpy188I	TCNGA	8 cut(s)	68, 206, 233, 396, 793, 955, 1267, 1362
Hpy188III	TCNNGA	5 cut(s)	550, 859, 962, 1136, 1197
Hpy8I	GTNNAC	5 cut(s)	196, 216, 1009, 1072, 1227
Hpy99I	CGWCG	4 cut(s)	188, 1207, 1366, 1369
HpyAV	CCTTC	5 cut(s)	284, 358, 605, 1099, 1307
HpyCH4III	ACNGT	2 cut(s)	177, 244
HpyCH4IV	ACGT	3 cut(s)	225, 597, 1068
HpyCH4V	TGCA	8 cut(s)	139, 283, 309, 388, 410, 1028, 1115, 1399
HpyF10VI	GCNNNNNNNGC	5 cut(s)	94, 318, 352, 668, 823
HpyF3I	CTNAG	7 cut(s)	65, 89, 148, 162, 584, 790, 1266
HpySE526I	ACGT	3 cut(s)	225, 597, 1068
Hsp92II	CATG	9 cut(s)	403, 475, 505, 553, 757, 862, 905, 926, 1179
HspAI	GCGC	2 cut(s)	190, 762
KpnI	GGTACC	1 cut(s)	1387
Kzo9I	GATC	6 cut(s)	420, 459, 492, 559, 1144, 1389
LmnI	GCTCC	1 cut(s)	326
Lsp1109I	GCAGC	6 cut(s)	81, 151, 321, 342, 381, 1411
LweI	GCATC	2 cut(s)	375, 1124
MaeI	CTAG	9 cut(s)	50, 75, 110, 533, 638, 672, 962, 1136, 1275
MaeII	ACGT	3 cut(s)	225, 597, 1068
MaeIII	GTNAC	2 cut(s)	114, 1309
MalI	GATC	6 cut(s)	422, 461, 494, 561, 1146, 1391
MboI	GATC	6 cut(s)	420, 459, 492, 559, 1144, 1389
MboII	GAAGA	8 cut(s)	4, 408, 610, 751, 929, 1285, 1352, 1390
MflI	RGATCY	1 cut(s)	492
MhlI	GDGCHC	1 cut(s)	323
MluCI	AATT	2 cut(s)	930, 1035
MlyI	GAGTC	2 cut(s)	127, 589
MmeI	TCCRAC	1 cut(s)	1385
Mph1103I	ATGCAT	1 cut(s)	390
MroXI	GAANNNNTTC	2 cut(s)	200, 299
MseI	TTAA	3 cut(s)	881, 1077, 1170
MslI	CAYNNNNRTG	1 cut(s)	1180
MspA1I	CMGCKG	1 cut(s)	1402
MspR9I	CCNGG	9 cut(s)	102, 170, 260, 317, 318, 481, 659, 736, 1260
Mva1269I	GAATGC	2 cut(s)	388, 785
MvaI	CCWGG	1 cut(s)	481
MvnI	CGCG	1 cut(s)	192
MwoI	GCNNNNNNNGC	5 cut(s)	94, 318, 352, 668, 823
NciI	CCSGG	8 cut(s)	102, 170, 260, 317, 318, 659, 736, 1260
NcoI	CCATGG	1 cut(s)	1175
NdeI	CATATG	1 cut(s)	1420
NdeII	GATC	6 cut(s)	420, 459, 492, 559, 1144, 1389
NlaIII	CATG	9 cut(s)	403, 475, 505, 553, 757, 862, 905, 926, 1179
NlaIV	GGNNCC	8 cut(s)	80, 107, 174, 208, 322, 431, 1329, 1385
NmeAIII	GCCGAG	1 cut(s)	1360
NmuCI	GTSAC	1 cut(s)	1309
NsiI	ATGCAT	1 cut(s)	390
NspI	RCATGY	1 cut(s)	757
PagI	TCATGA	2 cut(s)	549, 858
PciI	ACATGT	1 cut(s)	753
PcsI	WCGNNNNNNNCGW	1 cut(s)	189
PctI	GAATGC	2 cut(s)	388, 785
PdmI	GAANNNNTTC	2 cut(s)	200, 299
PfeI	GAWTC	1 cut(s)	476
PflMI	CCANNNNNTGG	2 cut(s)	439, 1101
PinAI	ACCGGT	2 cut(s)	198, 453
PkrI	GCNGC	6 cut(s)	96, 141, 311, 357, 371, 1401
PleI	GAGTC	2 cut(s)	127, 588
PmaCI	CACGTG	1 cut(s)	226
PmlI	CACGTG	1 cut(s)	226
PpsI	GAGTC	2 cut(s)	127, 588
Ppu21I	YACGTR	1 cut(s)	226
PscI	ACATGT	1 cut(s)	753
PsiI	TTATAA	1 cut(s)	978
Psp6I	CCWGG	1 cut(s)	479
PspCI	CACGTG	1 cut(s)	226
PspEI	GGTNACC	1 cut(s)	114
PspGI	CCWGG	1 cut(s)	479
PspN4I	GGNNCC	8 cut(s)	80, 107, 174, 208, 322, 431, 1329, 1385
PspPI	GGNCC	8 cut(s)	206, 430, 483, 573, 660, 898, 1199, 1244
PstI	CTGCAG	1 cut(s)	141
PsuI	RGATCY	1 cut(s)	492
RsaI	GTAC	3 cut(s)	596, 1131, 1385
RsaNI	GTAC	3 cut(s)	595, 1130, 1384
RseI	CAYNNNNRTG	1 cut(s)	1180
SaqAI	TTAA	3 cut(s)	881, 1077, 1170
SatI	GCNGC	6 cut(s)	95, 140, 310, 356, 370, 1400
Sau3AI	GATC	6 cut(s)	420, 459, 492, 559, 1144, 1389
Sau96I	GGNCC	8 cut(s)	206, 430, 483, 573, 660, 898, 1199, 1244
SchI	GAGTC	2 cut(s)	127, 589
ScrFI	CCNGG	9 cut(s)	102, 170, 260, 317, 318, 481, 659, 736, 1260
SduI	GDGCHC	1 cut(s)	323
SfaNI	GCATC	2 cut(s)	375, 1124
SfcI	CTRYAG	1 cut(s)	137
SinI	GGWCC	4 cut(s)	206, 430, 573, 1199
SmaI	CCCGGG	1 cut(s)	318
SmiMI	CAYNNNNRTG	1 cut(s)	1180
SmlI	CTYRAG	1 cut(s)	911
SmoI	CTYRAG	1 cut(s)	911
Sse9I	AATT	2 cut(s)	930, 1035
SsiI	CCGC	8 cut(s)	655, 669, 686, 774, 783, 1041, 1054, 1402
SspMI	CTAG	9 cut(s)	50, 75, 110, 533, 638, 672, 962, 1136, 1275
StyD4I	CCNGG	9 cut(s)	100, 168, 258, 315, 316, 479, 657, 734, 1258
StyI	CCWWGG	3 cut(s)	109, 1175, 1247
TaaI	ACNGT	2 cut(s)	177, 244
TaiI	ACGT	3 cut(s)	228, 600, 1071
TaqI	TCGA	5 cut(s)	183, 303, 419, 609, 1162
TaqII	GACCGA	2 cut(s)	590, 1216
TasI	AATT	2 cut(s)	930, 1035
TfiI	GAWTC	1 cut(s)	476
Tru1I	TTAA	3 cut(s)	881, 1077, 1170
Tru9I	TTAA	3 cut(s)	881, 1077, 1170
TscAI	CASTG	1 cut(s)	543
TseFI	GTSAC	1 cut(s)	1309
TseI	GCWGC	6 cut(s)	94, 139, 309, 355, 369, 1399
Tsp45I	GTSAC	1 cut(s)	1309
TspDTI	ATGAA	5 cut(s)	169, 265, 377, 582, 1289
TspGWI	ACGGA	5 cut(s)	397, 613, 899, 1079, 1279
TspMI	CCCGGG	1 cut(s)	316
TspRI	CASTG	1 cut(s)	543
Van91I	CCANNNNNTGG	2 cut(s)	439, 1101
VpaK11BI	GGWCC	4 cut(s)	206, 430, 573, 1199
XbaI	TCTAGA	2 cut(s)	961, 1135
XceI	RCATGY	1 cut(s)	757
XmaI	CCCGGG	1 cut(s)	316
XmaJI	CCTAGG	1 cut(s)	109
XmnI	GAANNNNTTC	2 cut(s)	200, 299
XspI	CTAG	9 cut(s)	50, 75, 110, 533, 638, 672, 962, 1136, 1275
Zsp2I	ATGCAT	1 cut(s)	390

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.