MD07G1226300.v1.1

Protein MID1-COMPLEMENTING ACTIVITY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0024479
Physical Location & Seq
Forward (+)
30209075 .. 30210045
971 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1226300.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGCGAGCTCAGATTTTGCAGAGGCACCGGGACTCGACGCCGTGGGCCTCACAAGCACGATTGTGTCCACCGCTCAAAGCGCCAGGACACACCGCCACAACTGCCAGCAGCTCGCGGAGCACGTAAGGATGGTAGGAAACTTGCTTGAGAAGATGAAATCAACGGACTTGATGAAGCTTCCATGTACAAAGGAGCCGTTGGATGGTCTTGAAAAGGCTCTTGTAAAAGCGCTTCTACTAGTACAGGGTTGCAGAGACAACAGCTGCCTCTACATGTTTGCCCTGGGATGGAGTGTGGTGTATCAATTCCGACAAGTCCAGGCGGAGATCGACCACTACGTCGGTCTCCTAGTCCGCCCCTTGGTTTCTCTGCTTCAAGAGTTTCGCCTTCAGAATTTGAAGGAAGGCTTGGAGGCCATTGAAGAGGATCAGAGGTTGTATACACTTGATGAACAGGACATGGAGGTTCATGGTGTGGTGTTGAAGCCTGATCGAACAACGAAAGATGCAGACATATTGGAGAGGGCGCTGTCTCGAAAATATCCCAACATGGCTTTTGATGAGGCACTCCAGGAGGAGAAAGAGAAGCTGAATATCGAACTACAGCGAGCAATAACGATTAACGATCCTGATCAATGCCGGGTGATTGAACATCTTATTCATGTAGCAGAAAATGTTGTGAGTGGTGTACTGCCAGGAAAGAAAGTTGAGAAGCTTCTTGTCAACGAACCAAGTTATGTGGTGTCAGGGTACGTAGTGTTTGAGGGATTGAGAGTACACCAGCCAATCACAAGTAATTTTTCGGTGCTACTGTCACACCAACACGTTACCAATACACTTATGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

283

Amino Acids

31.79

Weight (kDa)

5.5

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7792 PF25055 16 - 114 9.2e-07 Domain of unknown function (DUF7792)
MCAfunc PF19584 19 - 163 1.3e-43 MCAfunc domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024479)

Species Orthologous Gene IDs
malus_domestica MD07G1226300.v1.1
prunus_persica Prupe.2G255300_v2.0.a1
pyrus_communis pycom07g20460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 847
AasI GACNNNNNNGTC 1 cut(s) 338
AccB1I GGYRCC 1 cut(s) 25
AccBSI CCGCTC 1 cut(s) 74
AccI GTMKAC 1 cut(s) 440
AccII CGCG 1 cut(s) 116
AciI CCGC 5 cut(s) 72, 94, 116, 323, 355
AclWI GGATC 2 cut(s) 435, 620
AcsI RAATTY 1 cut(s) 394
AcuI CTGAAG 1 cut(s) 374
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 5 cut(s) 187, 243, 690, 752, 777
AfeI AGCGCT 1 cut(s) 231
AfiI CCNNNNNNNGG 3 cut(s) 203, 288, 361
AflIII ACRYGT 2 cut(s) 273, 823
AgsI TTSAA 6 cut(s) 212, 377, 400, 422, 484, 650
AhlI ACTAGT 1 cut(s) 238
AjnI CCWGG 5 cut(s) 83, 282, 318, 570, 694
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AleI CACNNNNGTG 1 cut(s) 62
AluBI AGCT 6 cut(s) 9, 112, 178, 264, 589, 715
AluI AGCT 6 cut(s) 9, 112, 178, 264, 589, 715
Alw21I GWGCWC 2 cut(s) 11, 123
Alw26I GTCTC 3 cut(s) 249, 350, 537
AlwI GGATC 2 cut(s) 435, 620
Aor51HI AGCGCT 1 cut(s) 231
AoxI GGCC 2 cut(s) 46, 414
ApeKI GCWGC 2 cut(s) 109, 264
ApoI RAATTY 1 cut(s) 394
AspLEI GCGC 3 cut(s) 83, 232, 529
AspS9I GGNCC 1 cut(s) 46
AsuC2I CCSGG 2 cut(s) 30, 641
AsuHPI GGTGA 1 cut(s) 655
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 1 cut(s) 11
BarI GAAGNNNNNNTAC 2 cut(s) 216, 248
Bbv12I GWGCWC 2 cut(s) 11, 123
BbvI GCAGC 2 cut(s) 121, 251
BccI CCATC 3 cut(s) 124, 197, 282
BceAI ACGGC 2 cut(s) 26, 181
BciT130I CCWGG 5 cut(s) 85, 284, 320, 572, 696
BclI TGATCA 1 cut(s) 631
BcnI CCSGG 2 cut(s) 30, 641
BcoDI GTCTC 3 cut(s) 249, 350, 537
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 2 cut(s) 239, 350
BfmI CTRYAG 1 cut(s) 602
BfoI RGCGCY 3 cut(s) 84, 233, 530
BisI GCNGC 2 cut(s) 110, 265
BlsI GCNGC 2 cut(s) 111, 266
Bme1390I CCNGG 7 cut(s) 30, 85, 284, 320, 572, 641, 696
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 2 cut(s) 27, 195
BmrFI CCNGG 7 cut(s) 30, 85, 284, 320, 572, 641, 696
BmsI GCATC 1 cut(s) 496
BpmI CTGGAG 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 2 cut(s) 30, 641
BsaAI YACGTR 2 cut(s) 124, 754
BsaBI GATNNNNATC 1 cut(s) 630
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 4 cut(s) 42, 282, 283, 360
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 288, 361
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 5 cut(s) 85, 284, 320, 572, 696
BseDI CCNNGG 4 cut(s) 42, 282, 283, 360
BseGI GGATG 3 cut(s) 135, 208, 293
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 3 cut(s) 203, 288, 361
BseMII CTCAG 1 cut(s) 24
BseRI GAGGAG 1 cut(s) 590
BseXI GCAGC 2 cut(s) 121, 251
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 2 cut(s) 48, 416
BshNI GGYRCC 1 cut(s) 25
BsiHKAI GWGCWC 2 cut(s) 11, 123
BsiSI CCGG 2 cut(s) 29, 640
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 3 cut(s) 203, 288, 361
BsmAI GTCTC 3 cut(s) 249, 350, 537
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 2 cut(s) 48, 416
Bso31I GGTCTC 1 cut(s) 350
Bsp1286I GDGCHC 2 cut(s) 11, 123
Bsp1407I TGTACA 1 cut(s) 185
Bsp143I GATC 5 cut(s) 327, 427, 490, 625, 631
BspACI CCGC 5 cut(s) 72, 94, 116, 323, 355
BspANI GGCC 2 cut(s) 48, 416
BspCNI CTCAG 1 cut(s) 23
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 2 cut(s) 27, 195
BspPI GGATC 2 cut(s) 435, 620
BspT107I GGYRCC 1 cut(s) 25
BspTNI GGTCTC 1 cut(s) 350
BsrBI CCGCTC 1 cut(s) 74
BsrGI TGTACA 1 cut(s) 185
BssECI CCNNGG 4 cut(s) 42, 282, 283, 360
BssMI GATC 5 cut(s) 327, 427, 490, 625, 631
BssNAI GTATAC 1 cut(s) 441
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 360
Bst1107I GTATAC 1 cut(s) 441
Bst2UI CCWGG 5 cut(s) 85, 284, 320, 572, 696
Bst4CI ACNGT 1 cut(s) 813
Bst6I CTCTTC 1 cut(s) 417
BstACI GRCGYC 1 cut(s) 39
BstAUI TGTACA 1 cut(s) 185
BstBAI YACGTR 2 cut(s) 124, 754
BstC8I GCNNGC 4 cut(s) 7, 107, 114, 609
BstDEI CTNAG 1 cut(s) 10
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 3 cut(s) 135, 208, 293
BstFNI CGCG 1 cut(s) 116
BstH2I RGCGCY 3 cut(s) 84, 233, 530
BstHHI GCGC 3 cut(s) 83, 232, 529
BstKTI GATC 5 cut(s) 330, 430, 493, 628, 634
BstMAI GTCTC 3 cut(s) 249, 350, 537
BstMBI GATC 5 cut(s) 327, 427, 490, 625, 631
BstMWI GCNNNNNNNGC 4 cut(s) 54, 80, 102, 118
BstNI CCWGG 5 cut(s) 85, 284, 320, 572, 696
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 7 cut(s) 28, 83, 282, 318, 570, 639, 694
BstSFI CTRYAG 1 cut(s) 602
BstSNI TACGTA 1 cut(s) 754
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 2 cut(s) 121, 251
BstZ17I GTATAC 1 cut(s) 441
BsuRI GGCC 2 cut(s) 48, 416
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 3 cut(s) 135, 208, 293
Cac8I GCNNGC 4 cut(s) 7, 107, 114, 609
CfoI GCGC 3 cut(s) 83, 232, 529
Cfr13I GGNCC 1 cut(s) 46
CseI GACGC 1 cut(s) 47
Csp6I GTAC 5 cut(s) 186, 242, 689, 751, 776
CspCI CAANNNNNGTGG 2 cut(s) 720, 755
CviAII CATG 6 cut(s) 183, 274, 460, 470, 550, 662
CviQI GTAC 5 cut(s) 186, 242, 689, 751, 776
DdeI CTNAG 1 cut(s) 10
DpnI GATC 5 cut(s) 329, 429, 492, 627, 633
DpnII GATC 5 cut(s) 327, 427, 490, 625, 631
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
EciI GGCGGA 2 cut(s) 338, 344
Ecl136II GAGCTC 1 cut(s) 9
Eco105I TACGTA 1 cut(s) 754
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 1 cut(s) 11
Eco31I GGTCTC 1 cut(s) 350
Eco47III AGCGCT 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 9
EcoRII CCWGG 5 cut(s) 83, 282, 318, 570, 694
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 6 cut(s) 186, 277, 463, 473, 553, 665
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FaqI GGGAC 1 cut(s) 45
FatI CATG 6 cut(s) 182, 273, 459, 469, 549, 661
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 1 cut(s) 440
Fnu4HI GCNGC 2 cut(s) 110, 265
FokI GGATG 3 cut(s) 142, 215, 300
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 2 cut(s) 110, 265
FspBI CTAG 2 cut(s) 239, 350
GlaI GCGC 3 cut(s) 82, 231, 528
GluI GCNGC 2 cut(s) 110, 265
GsuI CTGGAG 1 cut(s) 554
HaeII RGCGCY 3 cut(s) 84, 233, 530
HaeIII GGCC 2 cut(s) 48, 416
HapII CCGG 2 cut(s) 29, 640
HgaI GACGC 1 cut(s) 47
HhaI GCGC 3 cut(s) 83, 232, 529
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 6 cut(s) 186, 277, 463, 473, 553, 665
Hin6I GCGC 3 cut(s) 81, 230, 527
HinP1I GCGC 3 cut(s) 81, 230, 527
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HindIII AAGCTT 2 cut(s) 176, 713
HinfI GANTC 1 cut(s) 33
HpaII CCGG 2 cut(s) 29, 640
HphI GGTGA 1 cut(s) 655
Hpy166II GTNNAC 5 cut(s) 69, 441, 689, 724, 778
Hpy188I TCNGA 4 cut(s) 13, 311, 393, 432
Hpy188III TCNNGA 4 cut(s) 209, 377, 534, 629
Hpy8I GTNNAC 5 cut(s) 69, 441, 689, 724, 778
Hpy99I CGWCG 2 cut(s) 41, 344
HpyAV CCTTC 3 cut(s) 394, 398, 398
HpyCH4III ACNGT 1 cut(s) 813
HpyCH4IV ACGT 4 cut(s) 123, 339, 753, 825
HpyCH4V TGCA 3 cut(s) 20, 252, 509
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 80, 102, 118
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 4 cut(s) 123, 339, 753, 825
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 6 cut(s) 186, 277, 463, 473, 553, 665
HspAI GCGC 3 cut(s) 81, 230, 527
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 5 cut(s) 327, 427, 490, 625, 631
LmnI GCTCC 2 cut(s) 118, 193
Lsp1109I GCAGC 2 cut(s) 121, 251
LweI GCATC 1 cut(s) 496
MaeI CTAG 2 cut(s) 239, 350
MaeII ACGT 4 cut(s) 123, 339, 753, 825
MaeIII GTNAC 2 cut(s) 813, 826
MalI GATC 5 cut(s) 329, 429, 492, 627, 633
MbiI CCGCTC 1 cut(s) 74
MboI GATC 5 cut(s) 327, 427, 490, 625, 631
MboII GAAGA 2 cut(s) 163, 434
MhlI GDGCHC 2 cut(s) 11, 123
MluCI AATT 3 cut(s) 305, 394, 796
MlyI GAGTC 1 cut(s) 27
MmeI TCCRAC 2 cut(s) 180, 334
MseI TTAA 1 cut(s) 621
MslI CAYNNNNRTG 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 264
MspI CCGG 2 cut(s) 29, 640
MspR9I CCNGG 7 cut(s) 30, 85, 284, 320, 572, 641, 696
MvaI CCWGG 5 cut(s) 85, 284, 320, 572, 696
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 4 cut(s) 54, 80, 102, 118
NciI CCSGG 2 cut(s) 30, 641
NdeII GATC 5 cut(s) 327, 427, 490, 625, 631
NlaIII CATG 6 cut(s) 186, 277, 463, 473, 553, 665
NlaIV GGNNCC 2 cut(s) 27, 195
NmuCI GTSAC 1 cut(s) 813
NspI RCATGY 1 cut(s) 277
OliI CACNNNNGTG 1 cut(s) 62
PasI CCCWGGG 1 cut(s) 283
PciI ACATGT 1 cut(s) 273
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 336
PfoI TCCNGGA 1 cut(s) 570
PkrI GCNGC 2 cut(s) 111, 266
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
Ppu21I YACGTR 2 cut(s) 124, 754
PscI ACATGT 1 cut(s) 273
PsiI TTATAA 1 cut(s) 847
Psp124BI GAGCTC 1 cut(s) 11
Psp6I CCWGG 5 cut(s) 83, 282, 318, 570, 694
PspGI CCWGG 5 cut(s) 83, 282, 318, 570, 694
PspN4I GGNNCC 2 cut(s) 27, 195
PspPI GGNCC 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 264
RsaI GTAC 5 cut(s) 187, 243, 690, 752, 777
RsaNI GTAC 5 cut(s) 186, 242, 689, 751, 776
RseI CAYNNNNRTG 1 cut(s) 62
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 2 cut(s) 110, 265
Sau3AI GATC 5 cut(s) 327, 427, 490, 625, 631
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 27
ScrFI CCNGG 7 cut(s) 30, 85, 284, 320, 572, 641, 696
SduI GDGCHC 2 cut(s) 11, 123
SfaNI GCATC 1 cut(s) 496
SfcI CTRYAG 1 cut(s) 602
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SnaBI TACGTA 1 cut(s) 754
SpeI ACTAGT 1 cut(s) 238
Sse9I AATT 3 cut(s) 305, 394, 796
SsiI CCGC 5 cut(s) 72, 94, 116, 323, 355
SspMI CTAG 2 cut(s) 239, 350
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 7 cut(s) 28, 83, 282, 318, 570, 639, 694
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 1 cut(s) 813
TaiI ACGT 4 cut(s) 126, 342, 756, 828
TaqI TCGA 5 cut(s) 36, 330, 493, 535, 597
TaqII GACCGA 1 cut(s) 332
TasI AATT 3 cut(s) 305, 394, 796
TatI WGTACW 4 cut(s) 185, 241, 688, 775
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TseFI GTSAC 1 cut(s) 813
TseI GCWGC 2 cut(s) 109, 264
Tsp45I GTSAC 1 cut(s) 813
TspDTI ATGAA 5 cut(s) 170, 188, 458, 465, 650
TspGWI ACGGA 1 cut(s) 179
XapI RAATTY 1 cut(s) 394
XceI RCATGY 1 cut(s) 277
XmiI GTMKAC 1 cut(s) 440
XspI CTAG 2 cut(s) 239, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.