Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0009483

Physical Location & Seq

Genomic Coordinates Reverse (-)

34906761 .. 34911431

Gene Structure 4671 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1287700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGGAGTCTCTGAAATTCAGTAGCTTAATCCCATGCAAATCGTCGTCCTTAATTCCGGTTCCAACTAAGTTCACGAACCACCAGGCGATTGGGCAACTGGGTTTTGCTCCCCTGTGGAAAAGACGGTGCAGAAGAGTCGGTCTGCGGCTCAGAGCGTCGGTTGCCGGCGGAACTGGCGGCGGCGAGCAGGACGGCGCTTCTGCGGTCGAAGACCAGCAGAAGGACGAAAAGCGTGCTTTTTTGGGCGCCGAGAGAGACGATTCCGGCGCCTTTCTTGGGTTTAACTTGGTTCCTCCCAACGGTGAAACCAAAGTCAGTGACTCGCCCGAAGATGTTAGTTCTGTTGGAATGGAAGAATCAGTGGAAATAGATGGCAAAGAAACGTCCAAAGCAAGTGTAACACACAATGTTGTGTTTGTTACTGCCGAAGCTGCGCCTTATTCTAAGACGGGAGGCTTAGGAGATGTCTGTGGTTCTTTGCCTATTGTTCTAGCGGCACGTGGACACCGTGTTATGGTTGTTTCTCCTAGGTACCAAAACGGTACTGCTGCAGACCAGAAATATTCTGGTGCTGTTGATTTGGAAACAAGCATCAAGGCTTATTGCTTTGGTGGAGAGCAAGAAGTTTCTTTCTTCCATGAATATAGGGCAGGTGTTGATTGGGTATTCGTGGACCACCCGTCATACCACAGACCTGGAAATCCATACGGCGACAGTTTTGGTGCATTTGGTGACAATCAGTTTCGGTTCACTTTACTTTGCCATGCAGCGTGTGAAGCACCATTAGTGCTTCCACTGGGAGGGTTCATCTATGGCGAGAAAAGCCTGTTCATGGTCAATGACTGGCATGCCGGCCTTGTGCCACTGCTTTTGGCTGCCAATTATCGTCCATATGGGGTTTATAAGGACGCACGAAGTGTTCTTGTAATTCATAACCTTGCACATCAGGGAGTGGAACCGGGAGTAACATACAAGAATTTGGGACTACCTGCAGAGTGGTATGGAGCACTGGAATGGGTGTTTCCAACATGGGCAAGGACCCATGCTCTTGACACAGGTGAAGCAGTCAACATCCTGAAGGGTGCAATTGTGACATGTGATCGGATTCTGACAGTCAGCGAGGGCTACTCCTGGGAAATAACAACTGTTGAAGGTGGATATGGTCTAAATGAGCTACTAAGCAGTAGAAAGAGTGTTCTCACTGGAATAACGAATGGTGTTGATGTGGTTGAATGGAACCCATCTACTGATCTACACATTGCTTCCCGCTATTCTGCTGATGATCTTTCCGGAAAGGTTCAGTGCAAGCTTGCTTTGCAGAAGGAATTAGATCTTCCCATTAGGCCGGATTGCCCATTGATTGGATTCATTGGCCGACTGGACCACCAGAAAGGTATTGACCTGATTCAGTTAGGAATGCCACAGCTTATGGAAGATGACGTGCAATTTGTTATGCTGGGGTCTGGGGATCCACAGTGTGAAGATTGGATGAGAGCAACAGAAGCAACTTATAAAGATAAATTCCGGGGTTGGGTTGGATTTAACGTCCCAGTTTCTCATAGAATAACCGCAGGCTGCGACATACTCTTGATGCCGTCAAGATTTGAGCCCTGTGGACTTAATCAACTGTATGCAATGAGATATGGAACTGTACCAGTGGTTCATAGCACAGGAGGACTTAGAGATACAGTAATGAATTTCAATCCATATGCTAAAGGAGGCCAAGGTGACGGGACAGGGTGGACTTTTTCTCCATTGACGAAGGAGAGTATGCTGGCGGCACTAAGACTTGCTTGTAGGACTTTCACAGAGTACAAGCCTTCCTGGGAGGGATTGATGAAGAGATGCATGGAGAGGGACTTCACATGGGAAAGCGCTGCAATCAAGTACGAGCAGGTCTTCGAATGGGCGTTTATCGATCCACCCTACGTCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009011 GO:0009058 GO:0009059 GO:0009250 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010021 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046527 GO:0071704 GO:1901135 GO:1901137 GO:1901576 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026

Pfam Domains

Protein Families

Glyco_transf_5 Glycos_transf_1

Protein Analysis

646

Amino Acids

70.87

Weight (kDa)

5.54

Isoelectric Point (pI)

32.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_5	PF08323	136 - 394	4.9e-63	Starch synthase catalytic domain
Glycos_transf_1	PF00534	447 - 579	2.5e-13	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	452 - 564	6.7e-13	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009483)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24300 AT5G24300
fragaria_vesca	FvH4_7g31450 FvH4_7g31450
malus_domestica	MD01G1218000.v1.1 MD07G1287700.v1.1
prunus_persica	Prupe.2G309400_v2.0.a1
pyrus_communis	pycom01g22810 pycom07g26070
rosa_chinensis	RchiOBHm_Chr1g0380511
rosa_laevigata	RLG00000026290
rosa_multiflora	Rmu_sc0001733.1_g000001
rosa_roxburghii	Rroxscaffold_4G00279260
rosa_rugosa	Rorug01G0421400 Rorug01G0421500 Rorug01G0421600 Rorug01G0421700
rosa_samantha	Rh1AG446500 Rh1BG401700 Rh1CG416000 Rh1DG431900
rosa_wichuraiana	Rw1G038840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	903, 1512
AarI	CACCTGC	1 cut(s)	641
Acc36I	ACCTGC	3 cut(s)	641, 997, 1885
Acc65I	GGTACC	1 cut(s)	531
AccB1I	GGYRCC	3 cut(s)	245, 266, 531
AccB7I	CCANNNNNTGG	1 cut(s)	1361
AccIII	TCCGGA	1 cut(s)	1289
AciI	CCGC	9 cut(s)	145, 168, 177, 180, 203, 494, 1267, 1569, 1778
AclWI	GGATC	3 cut(s)	1463, 1476, 1913
AcoI	YGGCCR	1 cut(s)	1372
AcsI	RAATTY	4 cut(s)	14, 976, 1520, 1696
AcuI	CTGAAG	1 cut(s)	1097
AcvI	CACGTG	1 cut(s)	500
AcyI	GRCGYC	2 cut(s)	246, 267
AdeI	CACNNNGTG	2 cut(s)	917, 1478
AfaI	GTAC	5 cut(s)	533, 544, 1653, 1814, 1889
AfeI	AGCGCT	1 cut(s)	1876
AfiI	CCNNNNNNNGG	8 cut(s)	220, 276, 299, 514, 800, 832, 1361, 1531
AflIII	ACRYGT	1 cut(s)	1094
AgsI	TTSAA	3 cut(s)	1151, 1232, 1702
AhdI	GACNNNNNGTC	1 cut(s)	679
AjiI	CACGTC	1 cut(s)	1441
AjnI	CCWGG	4 cut(s)	81, 694, 1130, 1823
AluBI	AGCT	5 cut(s)	24, 431, 1174, 1309, 1426
AluI	AGCT	5 cut(s)	24, 431, 1174, 1309, 1426
Alw21I	GWGCWC	1 cut(s)	1009
Alw26I	GTCTC	2 cut(s)	12, 249
AlwI	GGATC	3 cut(s)	1463, 1476, 1913
Aor13HI	TCCGGA	1 cut(s)	1289
Aor51HI	AGCGCT	1 cut(s)	1876
AoxI	GGCC	4 cut(s)	853, 1343, 1372, 1720
ApeKI	GCWGC	6 cut(s)	431, 548, 767, 875, 1575, 1877
ApoI	RAATTY	4 cut(s)	14, 976, 1520, 1696
ArsI	GACNNNNNNTTYG	2 cut(s)	1430, 1462
Asp718I	GGTACC	1 cut(s)	531
AspA2I	CCTAGG	1 cut(s)	527
AspLEI	GCGC	5 cut(s)	197, 248, 269, 436, 1877
AspS9I	GGNCC	3 cut(s)	673, 1038, 1381
AsuC2I	CCSGG	2 cut(s)	960, 1526
AsuHPI	GGTGA	4 cut(s)	314, 743, 1070, 1739
AsuII	TTCGAA	1 cut(s)	1902
AvaII	GGWCC	3 cut(s)	673, 1038, 1381
AvrII	CCTAGG	1 cut(s)	527
BamHI	GGATCC	1 cut(s)	1468
BanI	GGYRCC	3 cut(s)	245, 266, 531
BanII	GRGCYC	1 cut(s)	1611
BbrPI	CACGTG	1 cut(s)	500
BbsI	GAAGAC	2 cut(s)	216, 1891
Bbv12I	GWGCWC	1 cut(s)	1009
BbvI	GCAGC	6 cut(s)	418, 535, 779, 862, 1562, 1864
BccI	CCATC	2 cut(s)	365, 1249
BceAI	ACGGC	3 cut(s)	208, 724, 1579
BcgI	CGANNNNNNTGC	4 cut(s)	118, 152, 215, 249
BciT130I	CCWGG	4 cut(s)	83, 696, 1132, 1825
BcnI	CCSGG	2 cut(s)	960, 1526
BcoDI	GTCTC	2 cut(s)	12, 249
BfaI	CTAG	2 cut(s)	491, 528
BfmI	CTRYAG	2 cut(s)	549, 990
BfoI	RGCGCY	4 cut(s)	198, 249, 270, 1878
BfuAI	ACCTGC	3 cut(s)	641, 997, 1885
BglII	AGATCT	1 cut(s)	1330
BlnI	CCTAGG	1 cut(s)	527
Bme1390I	CCNGG	6 cut(s)	83, 696, 960, 1132, 1526, 1825
Bme18I	GGWCC	3 cut(s)	673, 1038, 1381
BmeRI	GACNNNNNGTC	1 cut(s)	679
BmgBI	CACGTC	1 cut(s)	1441
BmgT120I	GGNCC	3 cut(s)	673, 1038, 1381
BmiI	GGNNCC	9 cut(s)	60, 247, 268, 291, 533, 957, 1040, 1238, 1470
BmrFI	CCNGG	6 cut(s)	83, 696, 960, 1132, 1526, 1825
BmrI	ACTGGG	3 cut(s)	107, 806, 1544
BmsI	GCATC	3 cut(s)	600, 1581, 1835
BmuI	ACTGGG	3 cut(s)	107, 806, 1544
BpiI	GAAGAC	2 cut(s)	216, 1891
BplI	GAGNNNNNCTC	2 cut(s)	1112, 1144
Bpu10I	CCTNAGC	1 cut(s)	457
Bpu14I	TTCGAA	1 cut(s)	1902
BpuMI	CCSGG	2 cut(s)	960, 1526
Bsa29I	ATCGAT	1 cut(s)	1917
BsaAI	YACGTR	1 cut(s)	500
BsaHI	GRCGYC	2 cut(s)	246, 267
BsaJI	CCNNGG	5 cut(s)	527, 1131, 1525, 1723, 1824
BsaWI	WCCGGW	2 cut(s)	55, 1289
BsaXI	ACNNNNNCTCC	2 cut(s)	1735, 1765
Bsc4I	CCNNNNNNNGG	8 cut(s)	220, 276, 299, 514, 800, 832, 1361, 1531
Bse118I	RCCGGY	2 cut(s)	164, 851
Bse1I	ACTGG	9 cut(s)	102, 178, 801, 848, 1014, 1207, 1383, 1550, 1655
Bse3DI	GCAATG	2 cut(s)	1257, 1641
BseAI	TCCGGA	1 cut(s)	1289
BseBI	CCWGG	4 cut(s)	83, 696, 1132, 1825
BseCI	ATCGAT	1 cut(s)	1917
BseDI	CCNNGG	5 cut(s)	527, 1131, 1525, 1723, 1824
BseGI	GGATG	2 cut(s)	1071, 1494
BseLI	CCNNNNNNNGG	8 cut(s)	220, 276, 299, 514, 800, 832, 1361, 1531
BseMI	GCAATG	2 cut(s)	1257, 1641
BseMII	CTCAG	1 cut(s)	163
BseNI	ACTGG	9 cut(s)	102, 178, 801, 848, 1014, 1207, 1383, 1550, 1655
BseXI	GCAGC	6 cut(s)	418, 535, 779, 862, 1562, 1864
BseYI	CCCAGC	1 cut(s)	1456
BsgI	GTGCAG	1 cut(s)	148
Bsh1285I	CGRYCG	1 cut(s)	207
BshFI	GGCC	4 cut(s)	855, 1345, 1374, 1722
BshNI	GGYRCC	3 cut(s)	245, 266, 531
BshVI	ATCGAT	1 cut(s)	1917
BsiEI	CGRYCG	1 cut(s)	207
BsiHKAI	GWGCWC	1 cut(s)	1009
BsiSI	CCGG	8 cut(s)	56, 165, 264, 852, 959, 1290, 1346, 1525
BslFI	GGGAC	4 cut(s)	996, 1532, 1747, 1871
BslI	CCNNNNNNNGG	8 cut(s)	220, 276, 299, 514, 800, 832, 1361, 1531
BsmAI	GTCTC	2 cut(s)	12, 249
BsmBI	CGTCTC	1 cut(s)	249
BsmFI	GGGAC	4 cut(s)	996, 1532, 1747, 1871
BsmI	GAATGC	1 cut(s)	1422
BsnI	GGCC	4 cut(s)	855, 1345, 1374, 1722
Bsp119I	TTCGAA	1 cut(s)	1902
Bsp1286I	GDGCHC	2 cut(s)	1009, 1611
Bsp13I	TCCGGA	1 cut(s)	1289
Bsp143I	GATC	6 cut(s)	1099, 1249, 1282, 1330, 1468, 1918
BspACI	CCGC	9 cut(s)	145, 168, 177, 180, 203, 494, 1267, 1569, 1778
BspANI	GGCC	4 cut(s)	855, 1345, 1374, 1722
BspCNI	CTCAG	1 cut(s)	162
BspDI	ATCGAT	1 cut(s)	1917
BspEI	TCCGGA	1 cut(s)	1289
BspLI	GGNNCC	9 cut(s)	60, 247, 268, 291, 533, 957, 1040, 1238, 1470
BspMAI	CTGCAG	2 cut(s)	553, 994
BspMI	ACCTGC	3 cut(s)	641, 997, 1885
BspPI	GGATC	3 cut(s)	1463, 1476, 1913
BspT104I	TTCGAA	1 cut(s)	1902
BspT107I	GGYRCC	3 cut(s)	245, 266, 531
BsrDI	GCAATG	2 cut(s)	1257, 1641
BsrFI	RCCGGY	2 cut(s)	164, 851
BsrI	ACTGG	9 cut(s)	102, 178, 801, 848, 1014, 1207, 1383, 1550, 1655
BssAI	RCCGGY	2 cut(s)	164, 851
BssECI	CCNNGG	5 cut(s)	527, 1131, 1525, 1723, 1824
BssMI	GATC	6 cut(s)	1099, 1249, 1282, 1330, 1468, 1918
BssNI	GRCGYC	2 cut(s)	246, 267
BssT1I	CCWWGG	2 cut(s)	527, 1723
Bst2UI	CCWGG	4 cut(s)	83, 696, 1132, 1825
Bst6I	CTCTTC	2 cut(s)	127, 1835
BstACI	GRCGYC	2 cut(s)	246, 267
BstBAI	YACGTR	1 cut(s)	500
BstBI	TTCGAA	1 cut(s)	1902
BstC8I	GCNNGC	9 cut(s)	166, 185, 234, 849, 853, 1307, 1311, 1573, 1776
BstDEI	CTNAG	7 cut(s)	66, 149, 444, 457, 1178, 1679, 1784
BstF5I	GGATG	2 cut(s)	1071, 1494
BstH2I	RGCGCY	4 cut(s)	198, 249, 270, 1878
BstHHI	GCGC	5 cut(s)	197, 248, 269, 436, 1877
BstKTI	GATC	6 cut(s)	1102, 1252, 1285, 1333, 1471, 1921
BstMAI	GTCTC	2 cut(s)	12, 249
BstMBI	GATC	6 cut(s)	1099, 1249, 1282, 1330, 1468, 1918
BstMCI	CGRYCG	1 cut(s)	207
BstMWI	GCNNNNNNNGC	7 cut(s)	161, 174, 431, 776, 822, 1315, 1502
BstNI	CCWGG	4 cut(s)	83, 696, 1132, 1825
BstNSI	RCATGY	2 cut(s)	851, 1098
BstSCI	CCNGG	6 cut(s)	81, 694, 958, 1130, 1524, 1823
BstSFI	CTRYAG	2 cut(s)	549, 990
BstV1I	GCAGC	6 cut(s)	418, 535, 779, 862, 1562, 1864
BstV2I	GAAGAC	2 cut(s)	216, 1891
BstX2I	RGATCY	2 cut(s)	1330, 1468
BstXI	CCANNNNNNTGG	2 cut(s)	89, 695
BstYI	RGATCY	2 cut(s)	1330, 1468
Bsu15I	ATCGAT	1 cut(s)	1917
BsuRI	GGCC	4 cut(s)	855, 1345, 1374, 1722
BsuTUI	ATCGAT	1 cut(s)	1917
BtrI	CACGTC	1 cut(s)	1441
BtsCI	GGATG	2 cut(s)	1071, 1494
BtsI	GCAGTG	1 cut(s)	863
BtsIMutI	CAGTG	9 cut(s)	322, 366, 794, 863, 1007, 1200, 1307, 1481, 1662
BveI	ACCTGC	3 cut(s)	641, 997, 1885
Cac8I	GCNNGC	9 cut(s)	166, 185, 234, 849, 853, 1307, 1311, 1573, 1776
CfoI	GCGC	5 cut(s)	197, 248, 269, 436, 1877
Cfr10I	RCCGGY	2 cut(s)	164, 851
Cfr13I	GGNCC	3 cut(s)	673, 1038, 1381
ClaI	ATCGAT	1 cut(s)	1917
CseI	GACGC	2 cut(s)	144, 917
Csp6I	GTAC	5 cut(s)	532, 543, 1652, 1813, 1888
CspCI	CAANNNNNGTGG	2 cut(s)	852, 887
CviQI	GTAC	5 cut(s)	532, 543, 1652, 1813, 1888
DdeI	CTNAG	7 cut(s)	66, 149, 444, 457, 1178, 1679, 1784
DinI	GGCGCC	2 cut(s)	247, 268
DpnI	GATC	6 cut(s)	1101, 1251, 1284, 1332, 1470, 1920
DpnII	GATC	6 cut(s)	1099, 1249, 1282, 1330, 1468, 1918
DraIII	CACNNNGTG	2 cut(s)	917, 1478
DriI	GACNNNNNGTC	1 cut(s)	679
EaeI	YGGCCR	1 cut(s)	1372
Eam1104I	CTCTTC	2 cut(s)	127, 1835
Eam1105I	GACNNNNNGTC	1 cut(s)	679
EarI	CTCTTC	2 cut(s)	127, 1835
EciI	GGCGGA	1 cut(s)	183
Eco130I	CCWWGG	2 cut(s)	527, 1723
Eco24I	GRGCYC	1 cut(s)	1611
Eco47I	GGWCC	3 cut(s)	673, 1038, 1381
Eco47III	AGCGCT	1 cut(s)	1876
Eco57I	CTGAAG	1 cut(s)	1097
Eco72I	CACGTG	1 cut(s)	500
EcoO109I	RGGNCCY	1 cut(s)	1038
EcoRII	CCWGG	4 cut(s)	81, 694, 1130, 1823
EcoT14I	CCWWGG	2 cut(s)	527, 1723
EcoT22I	ATGCAT	1 cut(s)	1850
EcoT38I	GRGCYC	1 cut(s)	1611
EgeI	GGCGCC	2 cut(s)	247, 268
EheI	GGCGCC	2 cut(s)	247, 268
ErhI	CCWWGG	2 cut(s)	527, 1723
Esp3I	CGTCTC	1 cut(s)	249
FalI	AAGNNNNNCTT	2 cut(s)	1777, 1809
FaqI	GGGAC	4 cut(s)	996, 1532, 1747, 1871
FauI	CCCGC	1 cut(s)	1274
FauNDI	CATATG	2 cut(s)	892, 1708
FokI	GGATG	2 cut(s)	1058, 1501
FriOI	GRGCYC	1 cut(s)	1611
FspBI	CTAG	2 cut(s)	491, 528
GlaI	GCGC	5 cut(s)	196, 247, 268, 435, 1876
GsaI	CCCAGC	1 cut(s)	1460
HaeII	RGCGCY	4 cut(s)	198, 249, 270, 1878
HaeIII	GGCC	4 cut(s)	855, 1345, 1374, 1722
HapII	CCGG	8 cut(s)	56, 165, 264, 852, 959, 1290, 1346, 1525
HgaI	GACGC	2 cut(s)	144, 917
HhaI	GCGC	5 cut(s)	197, 248, 269, 436, 1877
Hin1I	GRCGYC	2 cut(s)	246, 267
Hin6I	GCGC	5 cut(s)	195, 246, 267, 434, 1875
HinP1I	GCGC	5 cut(s)	195, 246, 267, 434, 1875
HincII	GTYRAC	1 cut(s)	1069
HindII	GTYRAC	1 cut(s)	1069
HindIII	AAGCTT	1 cut(s)	1307
HinfI	GANTC	8 cut(s)	5, 135, 260, 320, 356, 1105, 1365, 1405
HpaII	CCGG	8 cut(s)	56, 165, 264, 852, 959, 1290, 1346, 1525
HphI	GGTGA	4 cut(s)	314, 743, 1070, 1739
Hpy166II	GTNNAC	7 cut(s)	72, 503, 673, 750, 1069, 1616, 1743
Hpy188I	TCNGA	4 cut(s)	12, 152, 1104, 1110
Hpy188III	TCNNGA	6 cut(s)	73, 1049, 1075, 1290, 1588, 1599
Hpy8I	GTNNAC	7 cut(s)	72, 503, 673, 750, 1069, 1616, 1743
Hpy99I	CGWCG	2 cut(s)	46, 160
HpyAV	CCTTC	6 cut(s)	214, 1072, 1145, 1315, 1756, 1830
HpyCH4IV	ACGT	5 cut(s)	383, 499, 1440, 1545, 1929
HpyF10VI	GCNNNNNNNGC	7 cut(s)	161, 174, 431, 776, 822, 1315, 1502
HpyF3I	CTNAG	7 cut(s)	66, 149, 444, 457, 1178, 1679, 1784
HpySE526I	ACGT	5 cut(s)	383, 499, 1440, 1545, 1929
Hsp92I	GRCGYC	2 cut(s)	246, 267
HspAI	GCGC	5 cut(s)	195, 246, 267, 434, 1875
KasI	GGCGCC	2 cut(s)	245, 266
Kpn2I	TCCGGA	1 cut(s)	1289
KpnI	GGTACC	1 cut(s)	535
KroI	GCCGGC	2 cut(s)	164, 851
KroNI	GCCGGC	2 cut(s)	166, 853
Kzo9I	GATC	6 cut(s)	1099, 1249, 1282, 1330, 1468, 1918
LmnI	GCTCC	2 cut(s)	112, 1004
Lsp1109I	GCAGC	6 cut(s)	418, 535, 779, 862, 1562, 1864
LweI	GCATC	3 cut(s)	600, 1581, 1835
MaeI	CTAG	2 cut(s)	491, 528
MaeII	ACGT	5 cut(s)	383, 499, 1440, 1545, 1929
MaeIII	GTNAC	7 cut(s)	317, 397, 418, 731, 964, 1090, 1727
MalI	GATC	6 cut(s)	1101, 1251, 1284, 1332, 1470, 1920
MboI	GATC	6 cut(s)	1099, 1249, 1282, 1330, 1468, 1918
MfeI	CAATTG	1 cut(s)	1086
MflI	RGATCY	2 cut(s)	1330, 1468
MhlI	GDGCHC	2 cut(s)	1009, 1611
Mly113I	GGCGCC	2 cut(s)	246, 267
MlyI	GAGTC	3 cut(s)	14, 144, 314
MmeI	TCCRAC	4 cut(s)	86, 325, 1049, 1516
MnlI	CCTC	8 cut(s)	303, 446, 794, 1114, 1667, 1712, 1822, 1848
Mph1103I	ATGCAT	1 cut(s)	1850
MroI	TCCGGA	1 cut(s)	1289
MroNI	GCCGGC	2 cut(s)	164, 851
MseI	TTAA	5 cut(s)	26, 50, 282, 1542, 1620
MslI	CAYNNNNRTG	1 cut(s)	1012
MspI	CCGG	8 cut(s)	56, 165, 264, 852, 959, 1290, 1346, 1525
MspR9I	CCNGG	6 cut(s)	83, 696, 960, 1132, 1526, 1825
MunI	CAATTG	1 cut(s)	1086
Mva1269I	GAATGC	1 cut(s)	1422
MvaI	CCWGG	4 cut(s)	83, 696, 1132, 1825
MwoI	GCNNNNNNNGC	7 cut(s)	161, 174, 431, 776, 822, 1315, 1502
NaeI	GCCGGC	2 cut(s)	166, 853
NarI	GGCGCC	2 cut(s)	246, 267
NciI	CCSGG	2 cut(s)	960, 1526
NdeI	CATATG	2 cut(s)	892, 1708
NdeII	GATC	6 cut(s)	1099, 1249, 1282, 1330, 1468, 1918
NgoMIV	GCCGGC	2 cut(s)	164, 851
NlaIV	GGNNCC	9 cut(s)	60, 247, 268, 291, 533, 957, 1040, 1238, 1470
NmeAIII	GCCGAG	1 cut(s)	274
NmuCI	GTSAC	4 cut(s)	317, 731, 1090, 1727
NsiI	ATGCAT	1 cut(s)	1850
NspI	RCATGY	2 cut(s)	851, 1098
NspV	TTCGAA	1 cut(s)	1902
PaeI	GCATGC	1 cut(s)	851
PaqCI	CACCTGC	1 cut(s)	641
PciI	ACATGT	1 cut(s)	1094
PcsI	WCGNNNNNNNCGW	1 cut(s)	505
PctI	GAATGC	1 cut(s)	1422
PdiI	GCCGGC	2 cut(s)	166, 853
PfeI	GAWTC	5 cut(s)	260, 356, 1105, 1365, 1405
PflMI	CCANNNNNTGG	1 cut(s)	1361
PleI	GAGTC	3 cut(s)	13, 143, 314
PluTI	GGCGCC	2 cut(s)	249, 270
PmaCI	CACGTG	1 cut(s)	500
PmlI	CACGTG	1 cut(s)	500
PpsI	GAGTC	3 cut(s)	13, 143, 314
Ppu21I	YACGTR	1 cut(s)	500
PpuMI	RGGWCCY	1 cut(s)	1038
PscI	ACATGT	1 cut(s)	1094
PsiI	TTATAA	2 cut(s)	903, 1512
Psp5II	RGGWCCY	1 cut(s)	1038
Psp6I	CCWGG	4 cut(s)	81, 694, 1130, 1823
PspCI	CACGTG	1 cut(s)	500
PspFI	CCCAGC	1 cut(s)	1456
PspGI	CCWGG	4 cut(s)	81, 694, 1130, 1823
PspN4I	GGNNCC	9 cut(s)	60, 247, 268, 291, 533, 957, 1040, 1238, 1470
PspPI	GGNCC	3 cut(s)	673, 1038, 1381
PspPPI	RGGWCCY	1 cut(s)	1038
PsrI	GAACNNNNNNTAC	2 cut(s)	1644, 1676
PstI	CTGCAG	2 cut(s)	553, 994
PsuI	RGATCY	2 cut(s)	1330, 1468
RsaI	GTAC	5 cut(s)	533, 544, 1653, 1814, 1889
RsaNI	GTAC	5 cut(s)	532, 543, 1652, 1813, 1888
RseI	CAYNNNNRTG	1 cut(s)	1012
SaqAI	TTAA	5 cut(s)	26, 50, 282, 1542, 1620
Sau3AI	GATC	6 cut(s)	1099, 1249, 1282, 1330, 1468, 1918
Sau96I	GGNCC	3 cut(s)	673, 1038, 1381
SchI	GAGTC	3 cut(s)	14, 144, 314
ScrFI	CCNGG	6 cut(s)	83, 696, 960, 1132, 1526, 1825
SduI	GDGCHC	2 cut(s)	1009, 1611
SfaNI	GCATC	3 cut(s)	600, 1581, 1835
SfcI	CTRYAG	2 cut(s)	549, 990
SfoI	GGCGCC	2 cut(s)	247, 268
SfuI	TTCGAA	1 cut(s)	1902
SinI	GGWCC	3 cut(s)	673, 1038, 1381
SmiMI	CAYNNNNRTG	1 cut(s)	1012
SphI	GCATGC	1 cut(s)	851
SsiI	CCGC	9 cut(s)	145, 168, 177, 180, 203, 494, 1267, 1569, 1778
SspDI	GGCGCC	2 cut(s)	245, 266
SspI	AATATT	1 cut(s)	563
SspMI	CTAG	2 cut(s)	491, 528
StyD4I	CCNGG	6 cut(s)	81, 694, 958, 1130, 1524, 1823
StyI	CCWWGG	2 cut(s)	527, 1723
TaiI	ACGT	5 cut(s)	386, 502, 1443, 1548, 1932
TaqI	TCGA	3 cut(s)	207, 1902, 1917
TaqII	GACCGA	1 cut(s)	128
TatI	WGTACW	1 cut(s)	1812
TauI	GCSGC	5 cut(s)	148, 180, 183, 497, 1781
TfiI	GAWTC	5 cut(s)	260, 356, 1105, 1365, 1405
Tru1I	TTAA	5 cut(s)	26, 50, 282, 1542, 1620
Tru9I	TTAA	5 cut(s)	26, 50, 282, 1542, 1620
TscAI	CASTG	9 cut(s)	322, 366, 801, 870, 1014, 1207, 1307, 1481, 1662
TseFI	GTSAC	4 cut(s)	317, 731, 1090, 1727
TseI	GCWGC	6 cut(s)	431, 548, 767, 875, 1575, 1877
Tsp45I	GTSAC	4 cut(s)	317, 731, 1090, 1727
TspDTI	ATGAA	8 cut(s)	654, 796, 820, 920, 1357, 1652, 1709, 1853
TspRI	CASTG	9 cut(s)	322, 366, 801, 870, 1014, 1207, 1307, 1481, 1662
Van91I	CCANNNNNTGG	1 cut(s)	1361
VpaK11BI	GGWCC	3 cut(s)	673, 1038, 1381
XapI	RAATTY	4 cut(s)	14, 976, 1520, 1696
XceI	RCATGY	2 cut(s)	851, 1098
XcmI	CCANNNNNNNNNTGG	2 cut(s)	86, 563
XmaJI	CCTAGG	1 cut(s)	527
XspI	CTAG	2 cut(s)	491, 528
Zsp2I	ATGCAT	1 cut(s)	1850

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.