MD08G1063400.v1.1

Cytochrome b5-like Heme/Steroid binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016766
Physical Location & Seq
Reverse (-)
4975474 .. 4976823
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1063400.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGGCGGCAGAGTCAAAGAAGTACATTTCCCAGGAAAAACTCAGCACCCACAACAAGCCTGGAGATCTATGGATCTCGATACAGGGCAAGATCTACAACGTCTCAGATTGGGCCAACCACCATCCCGGCGGCGAGCTCCCGTTGCTCAGCCTCGCCGGCCAAGACGTCACGGACGCGTTCGTGGCCTACCACCCCGGCTCCGCCTGGCAGCACCTCGACCAGTTCTTCACCGGCTTCCATCTCGAAGACTATTCTGTTTCCGAAGTTTCCAAAGATTACAGGAAGCTCGTCAACGAGTTTACGAAAATGGGGCTTTTCGAAAACAGTGGACACAGCGTCCATTTCTATCTGTTTCTGGTGGCAATGCTGTTTACTCTGAGCATCTACGGCGTCTTGTGCTCCGACAGCGGCTGGGTTCATCTGGGTTCCGGCGTATTGATGGGTTTTCTCTGGATTCAAAGCGGGTGGCTCGGCCACGATTCGGGTCACTACCAGATCATCAGAAGCAAAAAGGTGAACAGATTCGCCCAGATCCTCACCGGAAACTGCCTCGCCGGCATCAGTATTGCGTGGTGGAAGCGCAACCACAACGCCCACCACATTTCCGTCAACAGCCTCGAATTCGATCCGGATCTCCAGCACATGCCATTTTTCGCGGTGAATTCGAAACTGTTCAGCTCCCTCGCCTCTTATTACTACGACAGGAAGATGAATTTCGATGCTTTTACTAGGTTCATGGTTAGTTACCAGCACTGGTCTTTTTACCCTGTCATGTGTCTTGCTAGGATTAACCTTTTTGCTCAGTCATTTTTACTGCTGTCATCCAAAAGATTGAGGGTAAAAAATAGGATCCAAGAAATCTTCGGGCTTTTGGTGTTTTGGATTTGGTATCCTCTGCTGGTTTCTTACCTTCCCAATTGGGGTGAAAGAGTAATGTTCGTTGTGGCAAGCTTTTCCGTCACTGGAATCCAACATGTGCAGTTCTGCTTGAACCATTTCTCTTCAAGTGTTTATGTCGGAACCCCTACAGGGAACGATTGGTGCGAAAATCAGACGAATGGATCGCTCGATATAGTCTGCCACCCGTGGATGGACTGGTTCCACGGCGGATTGCAGTTCCAGATCGAGCACCATTTGTTTCCGCGGTTGCCTCGAGGCAACCTCAGGAAAGTTGCCCCCCTTGTCAAGGAACTCTGCAAGAAGCACAAGCTGCTTTACACAAGTGTGTCATTCTACAAGGCCAATGAACTCACCGTTGGGACGCTGCGCAAGGCAGCGCTGCAGGCTCGCGATCTTGCCAGCCCGGTTCCAAAGAATTTAGTTTGGGAAGCTGTACACTCTGTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5 FA_desaturase

Protein Analysis

450

Amino Acids

51.48

Weight (kDa)

8.89

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 11 - 78 8.9e-22 Cytochrome b5-like Heme/Steroid binding domain
FA_desaturase PF00487 137 - 408 2.1e-37 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016766)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31760
malus_domestica MD08G1063400.v1.1
prunus_persica Prupe.1G411100_v2.0.a1
pyrus_communis pycom08g05210 pycom15g05510
rosa_chinensis RchiOBHm_Chr6g0311061
rosa_laevigata RLG00000010439
rosa_multiflora Rmu_sc0012235.1_g000004
rosa_roxburghii Rroxscaffold_7G00158060
rosa_rugosa Rorug06G0385000
rosa_samantha Rh6AG499000 Rh6BG509600 Rh6CG515400
rosa_wichuraiana Rw6G043460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
AbsI CCTCGAGG 1 cut(s) 1152
Acc16I TGCGCA 1 cut(s) 1268
AccII CGCG 4 cut(s) 176, 656, 1144, 1290
AccIII TCCGGA 1 cut(s) 628
AciI CCGC 9 cut(s) 5, 129, 201, 408, 462, 656, 1107, 1142, 1144
AclWI GGATC 7 cut(s) 80, 526, 620, 639, 844, 857, 1069
AcoI YGGCCR 2 cut(s) 157, 472
AcsI RAATTY 4 cut(s) 620, 661, 712, 1315
AcyI GRCGYC 2 cut(s) 165, 390
AfaI GTAC 2 cut(s) 23, 1335
AfeI AGCGCT 1 cut(s) 1278
AfiI CCNNNNNNNGG 5 cut(s) 481, 920, 1029, 1090, 1186
AflIII ACRYGT 2 cut(s) 174, 973
AgsI TTSAA 3 cut(s) 458, 991, 1005
AhdI GACNNNNNGTC 1 cut(s) 335
AjnI CCWGG 3 cut(s) 30, 58, 203
AjuI GAANNNNNNNTTGG 2 cut(s) 1239, 1271
AleI CACNNNNGTG 1 cut(s) 1223
AluBI AGCT 6 cut(s) 136, 286, 678, 951, 1210, 1331
AluI AGCT 6 cut(s) 136, 286, 678, 951, 1210, 1331
Alw21I GWGCWC 3 cut(s) 138, 401, 1131
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 7 cut(s) 80, 526, 620, 639, 844, 857, 1069
AlwNI CAGNNNCTG 1 cut(s) 411
Ama87I CYCGRG 1 cut(s) 1152
Aor13HI TCCGGA 1 cut(s) 628
Aor51HI AGCGCT 1 cut(s) 1278
AoxI GGCC 5 cut(s) 111, 157, 183, 472, 1239
ApeKI GCWGC 5 cut(s) 208, 1210, 1264, 1274, 1279
ApoI RAATTY 4 cut(s) 620, 661, 712, 1315
Asp700I GAANNNNTTC 3 cut(s) 521, 671, 995
AspLEI GCGC 3 cut(s) 582, 1269, 1279
AspS9I GGNCC 1 cut(s) 111
AsuC2I CCSGG 3 cut(s) 126, 195, 1304
AsuHPI GGTGA 6 cut(s) 220, 526, 529, 670, 935, 1243
AsuII TTCGAA 2 cut(s) 318, 665
AvaI CYCGRG 1 cut(s) 1152
AxyI CCTNAGG 1 cut(s) 1163
BamHI GGATCC 1 cut(s) 849
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 3 cut(s) 138, 401, 1131
BbvI GCAGC 5 cut(s) 220, 1197, 1251, 1266, 1286
BccI CCATC 4 cut(s) 129, 246, 433, 1084
BceAI ACGGC 2 cut(s) 403, 1120
BciT130I CCWGG 3 cut(s) 32, 60, 205
BciVI GTATCC 1 cut(s) 900
BcnI CCSGG 3 cut(s) 126, 195, 1304
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 2 cut(s) 729, 783
BfmI CTRYAG 2 cut(s) 1026, 1280
BfoI RGCGCY 1 cut(s) 1280
BfuI GTATCC 1 cut(s) 900
BglI GCCNNNNNGGC 2 cut(s) 156, 555
BglII AGATCT 2 cut(s) 64, 90
BisI GCNGC 8 cut(s) 6, 130, 209, 409, 1211, 1265, 1275, 1280
BlpI GCTNAGC 1 cut(s) 146
BlsI GCNGC 8 cut(s) 7, 131, 210, 410, 1212, 1266, 1276, 1281
Bme1390I CCNGG 6 cut(s) 32, 60, 126, 195, 205, 1304
BmeRI GACNNNNNGTC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 1152
BmgT120I GGNCC 1 cut(s) 111
BmiI GGNNCC 6 cut(s) 199, 427, 851, 1021, 1100, 1308
BmrFI CCNGG 6 cut(s) 32, 60, 126, 195, 205, 1304
BmsI GCATC 3 cut(s) 390, 567, 709
BpiI GAAGAC 1 cut(s) 252
BplI GAGNNNNNCTC 2 cut(s) 1146, 1178
BpmI CTGGAG 2 cut(s) 81, 620
Bpu1102I GCTNAGC 1 cut(s) 146
Bpu14I TTCGAA 2 cut(s) 318, 665
BpuMI CCSGG 3 cut(s) 126, 195, 1304
BsaBI GATNNNNATC 1 cut(s) 630
BsaHI GRCGYC 2 cut(s) 165, 390
BsaJI CCNNGG 5 cut(s) 30, 193, 1085, 1102, 1142
BsaWI WCCGGW 2 cut(s) 539, 628
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 5 cut(s) 481, 920, 1029, 1090, 1186
Bse118I RCCGGY 3 cut(s) 155, 230, 554
Bse1I ACTGG 4 cut(s) 220, 758, 967, 1100
Bse21I CCTNAGG 1 cut(s) 1163
Bse3DI GCAATG 1 cut(s) 369
Bse8I GATNNNNATC 1 cut(s) 630
BseAI TCCGGA 1 cut(s) 628
BseBI CCWGG 3 cut(s) 32, 60, 205
BseDI CCNNGG 5 cut(s) 30, 193, 1085, 1102, 1142
BseGI GGATG 3 cut(s) 121, 821, 1095
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 5 cut(s) 481, 920, 1029, 1090, 1186
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 6 cut(s) 55, 117, 160, 368, 815, 1177
BseNI ACTGG 4 cut(s) 220, 758, 967, 1100
BseXI GCAGC 5 cut(s) 220, 1197, 1251, 1266, 1286
BseYI CCCAGC 1 cut(s) 411
BsgI GTGCAG 1 cut(s) 998
Bsh1236I CGCG 4 cut(s) 176, 656, 1144, 1290
BshFI GGCC 5 cut(s) 113, 159, 185, 474, 1241
BsiHKAI GWGCWC 3 cut(s) 138, 401, 1131
BsiHKCI CYCGRG 1 cut(s) 1152
BsiSI CCGG 9 cut(s) 126, 156, 195, 231, 429, 540, 555, 629, 1304
BslFI GGGAC 1 cut(s) 1273
BslI CCNNNNNNNGG 5 cut(s) 481, 920, 1029, 1090, 1186
BsmAI GTCTC 1 cut(s) 106
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 1273
BsnI GGCC 5 cut(s) 113, 159, 185, 474, 1241
BsoBI CYCGRG 1 cut(s) 1152
Bsp119I TTCGAA 2 cut(s) 318, 665
Bsp1286I GDGCHC 3 cut(s) 138, 401, 1131
Bsp13I TCCGGA 1 cut(s) 628
Bsp1407I TGTACA 1 cut(s) 1333
Bsp1720I GCTNAGC 1 cut(s) 146
Bsp68I TCGCGA 1 cut(s) 1290
BspACI CCGC 9 cut(s) 5, 129, 201, 408, 462, 656, 1107, 1142, 1144
BspANI GGCC 5 cut(s) 113, 159, 185, 474, 1241
BspCNI CTCAG 6 cut(s) 54, 116, 159, 369, 814, 1176
BspEI TCCGGA 1 cut(s) 628
BspFNI CGCG 4 cut(s) 176, 656, 1144, 1290
BspLI GGNNCC 6 cut(s) 199, 427, 851, 1021, 1100, 1308
BspMAI CTGCAG 1 cut(s) 1284
BspPI GGATC 7 cut(s) 80, 526, 620, 639, 844, 857, 1069
BspT104I TTCGAA 2 cut(s) 318, 665
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 3 cut(s) 155, 230, 554
BsrGI TGTACA 1 cut(s) 1333
BsrI ACTGG 4 cut(s) 220, 758, 967, 1100
BssAI RCCGGY 3 cut(s) 155, 230, 554
BssECI CCNNGG 5 cut(s) 30, 193, 1085, 1102, 1142
BssNI GRCGYC 2 cut(s) 165, 390
Bst2UI CCWGG 3 cut(s) 32, 60, 205
Bst4CI ACNGT 3 cut(s) 326, 672, 1255
Bst6I CTCTTC 1 cut(s) 1006
BstACI GRCGYC 2 cut(s) 165, 390
BstAPI GCANNNNNTGC 1 cut(s) 1210
BstAUI TGTACA 1 cut(s) 1333
BstBI TTCGAA 2 cut(s) 318, 665
BstC8I GCNNGC 7 cut(s) 134, 157, 556, 949, 1284, 1288, 1300
BstDEI CTNAG 6 cut(s) 41, 103, 146, 377, 801, 1163
BstDSI CCRYGG 3 cut(s) 1085, 1102, 1142
BstENI CCTNNNNNAGG 1 cut(s) 1184
BstF5I GGATG 3 cut(s) 121, 821, 1095
BstFNI CGCG 4 cut(s) 176, 656, 1144, 1290
BstH2I RGCGCY 1 cut(s) 1280
BstHHI GCGC 3 cut(s) 582, 1269, 1279
BstMAI GTCTC 1 cut(s) 106
BstMWI GCNNNNNNNGC 9 cut(s) 142, 156, 182, 387, 396, 405, 555, 1210, 1283
BstNI CCWGG 3 cut(s) 32, 60, 205
BstNSI RCATGY 2 cut(s) 646, 977
BstSCI CCNGG 6 cut(s) 30, 58, 124, 193, 203, 1302
BstSFI CTRYAG 2 cut(s) 1026, 1280
BstUI CGCG 4 cut(s) 176, 656, 1144, 1290
BstV1I GCAGC 5 cut(s) 220, 1197, 1251, 1266, 1286
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 6 cut(s) 64, 72, 90, 531, 631, 849
BstYI RGATCY 6 cut(s) 64, 72, 90, 531, 631, 849
Bsu36I CCTNAGG 1 cut(s) 1163
BsuI GTATCC 1 cut(s) 900
BsuRI GGCC 5 cut(s) 113, 159, 185, 474, 1241
BtgI CCRYGG 3 cut(s) 1085, 1102, 1142
BtsCI GGATG 3 cut(s) 121, 821, 1095
BtsIMutI CAGTG 3 cut(s) 331, 751, 960
BtuMI TCGCGA 1 cut(s) 1290
Cac8I GCNNGC 7 cut(s) 134, 157, 556, 949, 1284, 1288, 1300
CaiI CAGNNNCTG 1 cut(s) 411
CfoI GCGC 3 cut(s) 582, 1269, 1279
Cfr10I RCCGGY 3 cut(s) 155, 230, 554
Cfr13I GGNCC 1 cut(s) 111
Cfr42I CCGCGG 1 cut(s) 1145
CseI GACGC 4 cut(s) 182, 325, 379, 1270
Csp6I GTAC 2 cut(s) 22, 1334
CspCI CAANNNNNGTGG 2 cut(s) 446, 481
CviAII CATG 4 cut(s) 643, 736, 772, 974
CviQI GTAC 2 cut(s) 22, 1334
DdeI CTNAG 6 cut(s) 41, 103, 146, 377, 801, 1163
DriI GACNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 2 cut(s) 157, 472
Eam1104I CTCTTC 1 cut(s) 1006
Eam1105I GACNNNNNGTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 1006
EciI GGCGGA 2 cut(s) 190, 1122
Ecl136II GAGCTC 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 138
Eco47III AGCGCT 1 cut(s) 1278
Eco53kI GAGCTC 1 cut(s) 136
Eco81I CCTNAGG 1 cut(s) 1163
Eco88I CYCGRG 1 cut(s) 1152
EcoICRI GAGCTC 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 1184
EcoRI GAATTC 2 cut(s) 620, 661
EcoRII CCWGG 3 cut(s) 30, 58, 203
EcoT38I GRGCYC 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 4 cut(s) 646, 739, 775, 977
FaiI YATR 7 cut(s) 70, 644, 737, 773, 975, 1014, 1073
FaqI GGGAC 1 cut(s) 1273
FatI CATG 4 cut(s) 642, 735, 771, 973
FauI CCCGC 1 cut(s) 455
Fnu4HI GCNGC 8 cut(s) 6, 130, 209, 409, 1211, 1265, 1275, 1280
FokI GGATG 3 cut(s) 108, 808, 1102
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 8 cut(s) 6, 130, 209, 409, 1211, 1265, 1275, 1280
FspBI CTAG 2 cut(s) 729, 783
FspI TGCGCA 1 cut(s) 1268
GlaI GCGC 3 cut(s) 581, 1268, 1278
GluI GCNGC 8 cut(s) 6, 130, 209, 409, 1211, 1265, 1275, 1280
GsaI CCCAGC 1 cut(s) 415
GsuI CTGGAG 2 cut(s) 81, 620
HaeII RGCGCY 1 cut(s) 1280
HaeIII GGCC 5 cut(s) 113, 159, 185, 474, 1241
HapII CCGG 9 cut(s) 126, 156, 195, 231, 429, 540, 555, 629, 1304
HgaI GACGC 4 cut(s) 182, 325, 379, 1270
HhaI GCGC 3 cut(s) 582, 1269, 1279
Hin1I GRCGYC 2 cut(s) 165, 390
Hin1II CATG 4 cut(s) 646, 739, 775, 977
Hin6I GCGC 3 cut(s) 580, 1267, 1277
HinP1I GCGC 3 cut(s) 580, 1267, 1277
HincII GTYRAC 2 cut(s) 292, 610
HindII GTYRAC 2 cut(s) 292, 610
HindIII AAGCTT 1 cut(s) 949
HinfI GANTC 5 cut(s) 11, 454, 479, 522, 966
HpaII CCGG 9 cut(s) 126, 156, 195, 231, 429, 540, 555, 629, 1304
HphI GGTGA 6 cut(s) 220, 526, 529, 670, 935, 1243
Hpy166II GTNNAC 7 cut(s) 292, 300, 329, 372, 517, 610, 1336
Hpy188I TCNGA 7 cut(s) 106, 262, 378, 403, 503, 1019, 1053
Hpy188III TCNNGA 7 cut(s) 76, 242, 451, 629, 1120, 1165, 1289
Hpy8I GTNNAC 7 cut(s) 292, 300, 329, 372, 517, 610, 1336
HpyAV CCTTC 1 cut(s) 920
HpyCH4III ACNGT 3 cut(s) 326, 672, 1255
HpyCH4IV ACGT 2 cut(s) 99, 165
HpyCH4V TGCA 4 cut(s) 979, 1114, 1197, 1282
HpyF10VI GCNNNNNNNGC 9 cut(s) 142, 156, 182, 387, 396, 405, 555, 1210, 1283
HpyF3I CTNAG 6 cut(s) 41, 103, 146, 377, 801, 1163
HpySE526I ACGT 2 cut(s) 99, 165
Hsp92I GRCGYC 2 cut(s) 165, 390
Hsp92II CATG 4 cut(s) 646, 739, 775, 977
HspAI GCGC 3 cut(s) 580, 1267, 1277
Kpn2I TCCGGA 1 cut(s) 628
KroI GCCGGC 2 cut(s) 155, 554
KroNI GCCGGC 2 cut(s) 157, 556
KspI CCGCGG 1 cut(s) 1145
LmnI GCTCC 4 cut(s) 141, 203, 404, 683
Lsp1109I GCAGC 5 cut(s) 220, 1197, 1251, 1266, 1286
LweI GCATC 3 cut(s) 390, 567, 709
MaeI CTAG 2 cut(s) 729, 783
MaeII ACGT 2 cut(s) 99, 165
MaeIII GTNAC 4 cut(s) 166, 485, 743, 958
MboII GAAGA 5 cut(s) 217, 257, 718, 853, 993
MfeI CAATTG 1 cut(s) 916
MflI RGATCY 6 cut(s) 64, 72, 90, 531, 631, 849
MhlI GDGCHC 3 cut(s) 138, 401, 1131
MluCI AATT 5 cut(s) 620, 661, 712, 916, 1315
MluI ACGCGT 1 cut(s) 174
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 3 cut(s) 426, 994, 997
MroI TCCGGA 1 cut(s) 628
MroNI GCCGGC 2 cut(s) 155, 554
MroXI GAANNNNTTC 3 cut(s) 521, 671, 995
MseI TTAA 1 cut(s) 789
MslI CAYNNNNRTG 1 cut(s) 1223
MspA1I CMGCKG 2 cut(s) 408, 1144
MspI CCGG 9 cut(s) 126, 156, 195, 231, 429, 540, 555, 629, 1304
MspR9I CCNGG 6 cut(s) 32, 60, 126, 195, 205, 1304
MunI CAATTG 1 cut(s) 916
MvaI CCWGG 3 cut(s) 32, 60, 205
MvnI CGCG 4 cut(s) 176, 656, 1144, 1290
MwoI GCNNNNNNNGC 9 cut(s) 142, 156, 182, 387, 396, 405, 555, 1210, 1283
NaeI GCCGGC 2 cut(s) 157, 556
NciI CCSGG 3 cut(s) 126, 195, 1304
NgoMIV GCCGGC 2 cut(s) 155, 554
NlaIII CATG 4 cut(s) 646, 739, 775, 977
NlaIV GGNNCC 6 cut(s) 199, 427, 851, 1021, 1100, 1308
NmeAIII GCCGAG 1 cut(s) 450
NmuCI GTSAC 3 cut(s) 166, 485, 958
NruI TCGCGA 1 cut(s) 1290
NsbI TGCGCA 1 cut(s) 1268
NspI RCATGY 2 cut(s) 646, 977
NspV TTCGAA 2 cut(s) 318, 665
OliI CACNNNNGTG 1 cut(s) 1223
PaeR7I CTCGAG 1 cut(s) 1152
PciI ACATGT 1 cut(s) 973
PcsI WCGNNNNNNNCGW 1 cut(s) 1041
PdiI GCCGGC 2 cut(s) 157, 556
PdmI GAANNNNTTC 3 cut(s) 521, 671, 995
PfeI GAWTC 4 cut(s) 454, 479, 522, 966
PkrI GCNGC 8 cut(s) 7, 131, 210, 410, 1212, 1266, 1276, 1281
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
PscI ACATGT 1 cut(s) 973
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 3 cut(s) 30, 58, 203
PspFI CCCAGC 1 cut(s) 411
PspGI CCWGG 3 cut(s) 30, 58, 203
PspN4I GGNNCC 6 cut(s) 199, 427, 851, 1021, 1100, 1308
PspPI GGNCC 1 cut(s) 111
PspXI VCTCGAGB 1 cut(s) 1152
PstI CTGCAG 1 cut(s) 1284
PstNI CAGNNNCTG 1 cut(s) 411
PsuI RGATCY 6 cut(s) 64, 72, 90, 531, 631, 849
RruI TCGCGA 1 cut(s) 1290
RsaI GTAC 2 cut(s) 23, 1335
RsaNI GTAC 2 cut(s) 22, 1334
RseI CAYNNNNRTG 1 cut(s) 1223
SacI GAGCTC 1 cut(s) 138
SacII CCGCGG 1 cut(s) 1145
SaqAI TTAA 1 cut(s) 789
SatI GCNGC 8 cut(s) 6, 130, 209, 409, 1211, 1265, 1275, 1280
Sau96I GGNCC 1 cut(s) 111
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 6 cut(s) 32, 60, 126, 195, 205, 1304
SduI GDGCHC 3 cut(s) 138, 401, 1131
SfaNI GCATC 3 cut(s) 390, 567, 709
SfcI CTRYAG 2 cut(s) 1026, 1280
Sfr274I CTCGAG 1 cut(s) 1152
Sfr303I CCGCGG 1 cut(s) 1145
SfuI TTCGAA 2 cut(s) 318, 665
SgrBI CCGCGG 1 cut(s) 1145
SlaI CTCGAG 1 cut(s) 1152
SmiMI CAYNNNNRTG 1 cut(s) 1223
SmlI CTYRAG 1 cut(s) 1152
SmoI CTYRAG 1 cut(s) 1152
Sse9I AATT 5 cut(s) 620, 661, 712, 916, 1315
SsiI CCGC 9 cut(s) 5, 129, 201, 408, 462, 656, 1107, 1142, 1144
SspMI CTAG 2 cut(s) 729, 783
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 6 cut(s) 30, 58, 124, 193, 203, 1302
TaaI ACNGT 3 cut(s) 326, 672, 1255
TaiI ACGT 2 cut(s) 102, 168
TasI AATT 5 cut(s) 620, 661, 712, 916, 1315
TatI WGTACW 2 cut(s) 21, 1333
TauI GCSGC 3 cut(s) 8, 132, 411
TfiI GAWTC 4 cut(s) 454, 479, 522, 966
Tru1I TTAA 1 cut(s) 789
Tru9I TTAA 1 cut(s) 789
TscAI CASTG 3 cut(s) 331, 758, 967
TseFI GTSAC 3 cut(s) 166, 485, 958
TseI GCWGC 5 cut(s) 208, 1210, 1264, 1274, 1279
Tsp45I GTSAC 3 cut(s) 166, 485, 958
TspDTI ATGAA 4 cut(s) 407, 724, 725, 1260
TspGWI ACGGA 3 cut(s) 185, 595, 946
TspRI CASTG 3 cut(s) 331, 758, 967
XagI CCTNNNNNAGG 1 cut(s) 1184
XapI RAATTY 4 cut(s) 620, 661, 712, 1315
XceI RCATGY 2 cut(s) 646, 977
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XhoI CTCGAG 1 cut(s) 1152
XmnI GAANNNNTTC 3 cut(s) 521, 671, 995
XspI CTAG 2 cut(s) 729, 783
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.