Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0022388

Physical Location & Seq

Genomic Coordinates Forward (+)

8546915 .. 8549334

Gene Structure 2420 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1101700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1278 bp

ATGAACTTCCTCCTTTCCTTCTGTTTTTGCCTCTGCACTTCTCTCTTGCCCTCCTTTGCAACCCCCGCCGTGTTATTCCAGGGGTTCAATTGGGATTCAAGTAAGAAAGGAGGATGGTACAACACACTCAAGACCTCCATTCCTGAACTTGCTGACTCTGGCATCACCCATGTTTGGCTTCCTCCTCCTTCTCACGCCAATGGAACTGAGGGATACTACCCTGGAAGGCTGTACGATCTCGATGCTTCGAGCTATGGAAATAAGGATGAATTGAAGTCCCTAATTACTGCCATGCATGAGAAGGGAATCAAAGCCATAGCAGACATTGTGATAAACCACAGAGCAGCTGTCAAACAAGATGAGAACGGAATATGGAGCATCTTCGAAGGCGGAACAGCGGACGGACGTCTTGACTTCAATGCCTCCTTGATTTGCAGGGATGACAATGACCATCCTTTTGGCACTGGCAACCCAGACACCGGAGACAACTTTCCATACACAGCTGACGTGGACCACACAAACCCTAGGCTTCAAAGTGAGTTGTCGGATTGGATGAACTGGTTGAAAACCGAAATAGGGTTCGACGGATGGAGGTTTGATTATGCCATTGGATACGGCTCTAGCTTCACCAAGCTCTACATGGATAGAACTTCACCAGAATTCGCAGTTGCCGAGTACTGGCGTTGGAACATTGCGAAAGGGCAGGACGGTAAACTCGAACAGAACCAAGATGCACATAGGAATGAAATAGCTAGTTGGATTCAGGCTGCTGGGGGTGTTGTCACGGCATTTGATATGACAACCAAGTATATTATGGATGTTGCTGTGGTGGGTGAGCTATGGAGGTTGAAGGATTCAAATGGCAAACCACCAGGGTTGATTGGGATCAAGCCAGAAAATGCTGTGACGTTTATCGACAATCATGACACTTGGTCTCAAAAACTCTTGCCTTTCCCATCTGATCAAGTCCTGCTTGGATACGCTTACATTCTCACCCATCCTGGGACCCCAAGCATATTCTATGATCACTTCTTTGAGTGGGGGTGGCCAAAGGAGCCTATACGCAACTTGACTGCAATTAGGGCAAGGAATGGGATCAATTCCAAGAGCAGTGTGAGTATCTTAGCTGCCGAGGCTAACCTGTATATGGCCAACATTGACGACAAGATTATCATGAAGATTGGTACAAAGTTGGACCTTGGAGACCTTGATCCCACAAAGTCGAACTTCCACGTTGCCACCAGTGGACAAGACTTTGCTGTGTGGGAGAGAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004556 GO:0005575 GO:0005576 GO:0005615 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009987 GO:0016052 GO:0016160 GO:0016787 GO:0016798 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044421 GO:0046352 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko04973 map00500 map01100 map04973

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

426

Amino Acids

47.55

Weight (kDa)

5.09

Isoelectric Point (pI)

31.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	49 - 116	6.7e-09	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	363 - 423	1.9e-22	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0022388)

Species	Orthologous Gene IDs
malus_domestica	MD08G1101700.v1.1
prunus_persica	Prupe.1G435600_v2.0.a1
pyrus_communis	pycom08g08220 pycom15g07760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	409
AciI	CCGC	3 cut(s)	66, 390, 398
AclWI	GGATC	3 cut(s)	893, 1103, 1205
AcoI	YGGCCR	2 cut(s)	1046, 1149
AcsI	RAATTY	1 cut(s)	659
AcyI	GRCGYC	1 cut(s)	406
AfaI	GTAC	4 cut(s)	119, 233, 677, 1186
AfiI	CCNNNNNNNGG	6 cut(s)	174, 479, 576, 678, 1002, 1147
AgsI	TTSAA	8 cut(s)	88, 99, 274, 418, 533, 565, 850, 858
AhdI	GACNNNNNGTC	1 cut(s)	931
AjiI	CACGTC	1 cut(s)	508
AjnI	CCWGG	4 cut(s)	78, 220, 871, 1000
AluBI	AGCT	8 cut(s)	252, 347, 503, 624, 634, 752, 838, 1127
AluI	AGCT	8 cut(s)	252, 347, 503, 624, 634, 752, 838, 1127
Alw26I	GTCTC	3 cut(s)	477, 939, 1197
AlwI	GGATC	3 cut(s)	893, 1103, 1205
AoxI	GGCC	2 cut(s)	1046, 1149
ApeKI	GCWGC	3 cut(s)	344, 767, 1127
ApoI	RAATTY	1 cut(s)	659
ArsI	GACNNNNNNTTYG	2 cut(s)	440, 472
AspA2I	CCTAGG	1 cut(s)	524
AspS9I	GGNCC	3 cut(s)	511, 1005, 1195
AsuHPI	GGTGA	5 cut(s)	157, 619, 645, 845, 985
AsuII	TTCGAA	1 cut(s)	384
AvaII	GGWCC	3 cut(s)	511, 1005, 1195
AvrII	CCTAGG	1 cut(s)	524
BalI	TGGCCA	2 cut(s)	1048, 1151
BbvI	GCAGC	3 cut(s)	356, 754, 1114
BccI	CCATC	5 cut(s)	108, 459, 582, 964, 1005
BceAI	ACGGC	3 cut(s)	53, 631, 801
BcgI	CGANNNNNNTGC	2 cut(s)	224, 258
BciT130I	CCWGG	4 cut(s)	80, 222, 873, 1002
BciVI	GTATCC	3 cut(s)	206, 605, 971
BclI	TGATCA	2 cut(s)	961, 1024
BcoDI	GTCTC	3 cut(s)	477, 939, 1197
BfaI	CTAG	3 cut(s)	525, 621, 753
BfuI	GTATCC	3 cut(s)	206, 605, 971
BisI	GCNGC	3 cut(s)	345, 768, 1128
BlnI	CCTAGG	1 cut(s)	524
BlsI	GCNGC	3 cut(s)	346, 769, 1129
BmcAI	AGTACT	1 cut(s)	677
Bme1390I	CCNGG	4 cut(s)	80, 222, 873, 1002
Bme18I	GGWCC	3 cut(s)	511, 1005, 1195
BmeRI	GACNNNNNGTC	1 cut(s)	931
BmgBI	CACGTC	1 cut(s)	508
BmgT120I	GGNCC	3 cut(s)	511, 1005, 1195
BmiI	GGNNCC	3 cut(s)	1006, 1007, 1056
BmrFI	CCNGG	4 cut(s)	80, 222, 873, 1002
BmsI	GCATC	4 cut(s)	171, 232, 387, 721
BoxI	GACNNNNGTC	1 cut(s)	405
Bpu14I	TTCGAA	1 cut(s)	384
BpuEI	CTTGAG	1 cut(s)	113
BsaBI	GATNNNNATC	1 cut(s)	884
BsaHI	GRCGYC	1 cut(s)	406
BsaI	GGTCTC	2 cut(s)	939, 1197
BsaJI	CCNNGG	7 cut(s)	79, 220, 524, 872, 1001, 1131, 1198
BsaWI	WCCGGW	1 cut(s)	479
Bsc4I	CCNNNNNNNGG	6 cut(s)	174, 479, 576, 678, 1002, 1147
Bse1I	ACTGG	4 cut(s)	469, 563, 683, 1242
Bse3DI	GCAATG	1 cut(s)	690
Bse8I	GATNNNNATC	1 cut(s)	884
BseBI	CCWGG	4 cut(s)	80, 222, 873, 1002
BseDI	CCNNGG	7 cut(s)	79, 220, 524, 872, 1001, 1131, 1198
BseGI	GGATG	8 cut(s)	119, 271, 445, 451, 558, 593, 823, 997
BseJI	GATNNNNATC	1 cut(s)	884
BseLI	CCNNNNNNNGG	6 cut(s)	174, 479, 576, 678, 1002, 1147
BseMI	GCAATG	1 cut(s)	690
BseMII	CTCAG	1 cut(s)	198
BseNI	ACTGG	4 cut(s)	469, 563, 683, 1242
BseRI	GAGGAG	1 cut(s)	174
BseXI	GCAGC	3 cut(s)	356, 754, 1114
BseYI	CCCAGC	1 cut(s)	770
BsgI	GTGCAG	1 cut(s)	19
BshFI	GGCC	2 cut(s)	1048, 1151
BsiSI	CCGG	1 cut(s)	480
BslFI	GGGAC	2 cut(s)	262, 1018
BslI	CCNNNNNNNGG	6 cut(s)	174, 479, 576, 678, 1002, 1147
BsmAI	GTCTC	3 cut(s)	477, 939, 1197
BsmFI	GGGAC	2 cut(s)	262, 1018
BsnI	GGCC	2 cut(s)	1048, 1151
Bso31I	GGTCTC	2 cut(s)	939, 1197
Bsp119I	TTCGAA	1 cut(s)	384
Bsp143I	GATC	6 cut(s)	235, 885, 961, 1024, 1095, 1210
BspACI	CCGC	3 cut(s)	66, 390, 398
BspANI	GGCC	2 cut(s)	1048, 1151
BspCNI	CTCAG	1 cut(s)	199
BspHI	TCATGA	2 cut(s)	922, 1173
BspLI	GGNNCC	3 cut(s)	1006, 1007, 1056
BspPI	GGATC	3 cut(s)	893, 1103, 1205
BspT104I	TTCGAA	1 cut(s)	384
BspTNI	GGTCTC	2 cut(s)	939, 1197
BsrDI	GCAATG	1 cut(s)	690
BsrI	ACTGG	4 cut(s)	469, 563, 683, 1242
BssECI	CCNNGG	7 cut(s)	79, 220, 524, 872, 1001, 1131, 1198
BssMI	GATC	6 cut(s)	235, 885, 961, 1024, 1095, 1210
BssNI	GRCGYC	1 cut(s)	406
BssT1I	CCWWGG	2 cut(s)	524, 1198
Bst2UI	CCWGG	4 cut(s)	80, 222, 873, 1002
Bst4CI	ACNGT	1 cut(s)	710
BstACI	GRCGYC	1 cut(s)	406
BstBI	TTCGAA	1 cut(s)	384
BstDEI	CTNAG	2 cut(s)	207, 1123
BstF5I	GGATG	8 cut(s)	119, 271, 445, 451, 558, 593, 823, 997
BstKTI	GATC	6 cut(s)	238, 888, 964, 1027, 1098, 1213
BstMAI	GTCTC	3 cut(s)	477, 939, 1197
BstMBI	GATC	6 cut(s)	235, 885, 961, 1024, 1095, 1210
BstMWI	GCNNNNNNNGC	4 cut(s)	65, 1054, 1082, 1133
BstNI	CCWGG	4 cut(s)	80, 222, 873, 1002
BstPAI	GACNNNNGTC	1 cut(s)	405
BstSCI	CCNGG	4 cut(s)	78, 220, 871, 1000
BstV1I	GCAGC	3 cut(s)	356, 754, 1114
BstXI	CCANNNNNNTGG	1 cut(s)	458
BsuI	GTATCC	3 cut(s)	206, 605, 971
BsuRI	GGCC	2 cut(s)	1048, 1151
BtrI	CACGTC	1 cut(s)	508
BtsCI	GGATG	8 cut(s)	119, 271, 445, 451, 558, 593, 823, 997
BtsI	GCAGTG	1 cut(s)	1117
BtsIMutI	CAGTG	3 cut(s)	462, 1117, 1249
CciI	TCATGA	2 cut(s)	922, 1173
Cfr13I	GGNCC	3 cut(s)	511, 1005, 1195
Csp6I	GTAC	4 cut(s)	118, 232, 676, 1185
CspCI	CAANNNNNGTGG	2 cut(s)	858, 893
CviAII	CATG	6 cut(s)	170, 292, 296, 640, 923, 1174
CviQI	GTAC	4 cut(s)	118, 232, 676, 1185
DdeI	CTNAG	2 cut(s)	207, 1123
DpnI	GATC	6 cut(s)	237, 887, 963, 1026, 1097, 1212
DpnII	GATC	6 cut(s)	235, 885, 961, 1024, 1095, 1210
DriI	GACNNNNNGTC	1 cut(s)	931
EaeI	YGGCCR	2 cut(s)	1046, 1149
Eam1105I	GACNNNNNGTC	1 cut(s)	931
EciI	GGCGGA	1 cut(s)	405
Eco130I	CCWWGG	2 cut(s)	524, 1198
Eco31I	GGTCTC	2 cut(s)	939, 1197
Eco47I	GGWCC	3 cut(s)	511, 1005, 1195
EcoO109I	RGGNCCY	1 cut(s)	1005
EcoRI	GAATTC	1 cut(s)	659
EcoRII	CCWGG	4 cut(s)	78, 220, 871, 1000
EcoT14I	CCWWGG	2 cut(s)	524, 1198
EcoT22I	ATGCAT	1 cut(s)	297
ErhI	CCWWGG	2 cut(s)	524, 1198
FaeI	CATG	6 cut(s)	173, 295, 299, 643, 926, 1177
FalI	AAGNNNNNCTT	4 cut(s)	957, 989, 1211, 1243
FaqI	GGGAC	2 cut(s)	262, 1018
FatI	CATG	6 cut(s)	169, 291, 295, 639, 922, 1173
FauI	CCCGC	1 cut(s)	73
FbaI	TGATCA	2 cut(s)	961, 1024
Fnu4HI	GCNGC	3 cut(s)	345, 768, 1128
FokI	GGATG	8 cut(s)	126, 278, 438, 452, 565, 600, 830, 984
Fsp4HI	GCNGC	3 cut(s)	345, 768, 1128
FspBI	CTAG	3 cut(s)	525, 621, 753
GluI	GCNGC	3 cut(s)	345, 768, 1128
GsaI	CCCAGC	1 cut(s)	774
HaeIII	GGCC	2 cut(s)	1048, 1151
HapII	CCGG	1 cut(s)	480
Hin1I	GRCGYC	1 cut(s)	406
Hin1II	CATG	6 cut(s)	173, 295, 299, 643, 926, 1177
HinfI	GANTC	5 cut(s)	95, 155, 306, 760, 854
HpaII	CCGG	1 cut(s)	480
HphI	GGTGA	5 cut(s)	157, 619, 645, 845, 985
Hpy166II	GTNNAC	3 cut(s)	511, 713, 1247
Hpy188I	TCNGA	2 cut(s)	547, 961
Hpy188III	TCNNGA	6 cut(s)	130, 143, 239, 410, 923, 1174
Hpy8I	GTNNAC	3 cut(s)	511, 713, 1247
Hpy99I	CGWCG	1 cut(s)	587
HpyAV	CCTTC	6 cut(s)	28, 198, 219, 295, 380, 844
HpyCH4III	ACNGT	1 cut(s)	710
HpyCH4IV	ACGT	4 cut(s)	406, 507, 908, 1233
HpyCH4V	TGCA	6 cut(s)	36, 59, 295, 435, 734, 1076
HpyF10VI	GCNNNNNNNGC	4 cut(s)	65, 1054, 1082, 1133
HpyF3I	CTNAG	2 cut(s)	207, 1123
HpySE526I	ACGT	4 cut(s)	406, 507, 908, 1233
Hsp92I	GRCGYC	1 cut(s)	406
Hsp92II	CATG	6 cut(s)	173, 295, 299, 643, 926, 1177
KflI	GGGWCCC	1 cut(s)	1005
Ksp22I	TGATCA	2 cut(s)	961, 1024
Kzo9I	GATC	6 cut(s)	235, 885, 961, 1024, 1095, 1210
LmnI	GCTCC	2 cut(s)	375, 1054
Lsp1109I	GCAGC	3 cut(s)	356, 754, 1114
LweI	GCATC	4 cut(s)	171, 232, 387, 721
MaeI	CTAG	3 cut(s)	525, 621, 753
MaeII	ACGT	4 cut(s)	406, 507, 908, 1233
MaeIII	GTNAC	2 cut(s)	781, 904
MalI	GATC	6 cut(s)	237, 887, 963, 1026, 1097, 1212
MboI	GATC	6 cut(s)	235, 885, 961, 1024, 1095, 1210
MboII	GAAGA	2 cut(s)	373, 1189
MfeI	CAATTG	1 cut(s)	88
MlsI	TGGCCA	2 cut(s)	1048, 1151
MluCI	AATT	6 cut(s)	88, 269, 282, 659, 1077, 1099
MluNI	TGGCCA	2 cut(s)	1048, 1151
MlyI	GAGTC	1 cut(s)	149
MmeI	TCCRAC	4 cut(s)	525, 665, 737, 1173
Mox20I	TGGCCA	2 cut(s)	1048, 1151
Mph1103I	ATGCAT	1 cut(s)	297
MscI	TGGCCA	2 cut(s)	1048, 1151
MslI	CAYNNNNRTG	2 cut(s)	198, 741
Msp20I	TGGCCA	2 cut(s)	1048, 1151
MspA1I	CMGCKG	3 cut(s)	347, 398, 503
MspI	CCGG	1 cut(s)	480
MspR9I	CCNGG	4 cut(s)	80, 222, 873, 1002
MunI	CAATTG	1 cut(s)	88
MvaI	CCWGG	4 cut(s)	80, 222, 873, 1002
MwoI	GCNNNNNNNGC	4 cut(s)	65, 1054, 1082, 1133
NdeII	GATC	6 cut(s)	235, 885, 961, 1024, 1095, 1210
NlaIII	CATG	6 cut(s)	173, 295, 299, 643, 926, 1177
NlaIV	GGNNCC	3 cut(s)	1006, 1007, 1056
NmeAIII	GCCGAG	2 cut(s)	697, 1156
NmuCI	GTSAC	2 cut(s)	781, 904
NsiI	ATGCAT	1 cut(s)	297
NspV	TTCGAA	1 cut(s)	384
PagI	TCATGA	2 cut(s)	922, 1173
PcsI	WCGNNNNNNNCGW	2 cut(s)	669, 714
PfeI	GAWTC	4 cut(s)	95, 306, 760, 854
PkrI	GCNGC	3 cut(s)	346, 769, 1129
PleI	GAGTC	1 cut(s)	149
PpsI	GAGTC	1 cut(s)	149
PpuMI	RGGWCCY	1 cut(s)	1005
PshAI	GACNNNNGTC	1 cut(s)	405
Psp5II	RGGWCCY	1 cut(s)	1005
Psp6I	CCWGG	4 cut(s)	78, 220, 871, 1000
PspFI	CCCAGC	1 cut(s)	770
PspGI	CCWGG	4 cut(s)	78, 220, 871, 1000
PspN4I	GGNNCC	3 cut(s)	1006, 1007, 1056
PspPI	GGNCC	3 cut(s)	511, 1005, 1195
PspPPI	RGGWCCY	1 cut(s)	1005
PvuII	CAGCTG	2 cut(s)	347, 503
RsaI	GTAC	4 cut(s)	119, 233, 677, 1186
RsaNI	GTAC	4 cut(s)	118, 232, 676, 1185
RseI	CAYNNNNRTG	2 cut(s)	198, 741
SatI	GCNGC	3 cut(s)	345, 768, 1128
Sau3AI	GATC	6 cut(s)	235, 885, 961, 1024, 1095, 1210
Sau96I	GGNCC	3 cut(s)	511, 1005, 1195
ScaI	AGTACT	1 cut(s)	677
SchI	GAGTC	1 cut(s)	149
ScrFI	CCNGG	4 cut(s)	80, 222, 873, 1002
SfaNI	GCATC	4 cut(s)	171, 232, 387, 721
SfuI	TTCGAA	1 cut(s)	384
SinI	GGWCC	3 cut(s)	511, 1005, 1195
SmiMI	CAYNNNNRTG	2 cut(s)	198, 741
SmlI	CTYRAG	1 cut(s)	128
SmoI	CTYRAG	1 cut(s)	128
Sse9I	AATT	6 cut(s)	88, 269, 282, 659, 1077, 1099
SsiI	CCGC	3 cut(s)	66, 390, 398
SspMI	CTAG	3 cut(s)	525, 621, 753
StyD4I	CCNGG	4 cut(s)	78, 220, 871, 1000
StyI	CCWWGG	2 cut(s)	524, 1198
TaaI	ACNGT	1 cut(s)	710
TaiI	ACGT	4 cut(s)	409, 510, 911, 1236
TaqI	TCGA	7 cut(s)	240, 248, 384, 582, 717, 915, 1223
TasI	AATT	6 cut(s)	88, 269, 282, 659, 1077, 1099
TatI	WGTACW	1 cut(s)	675
TfiI	GAWTC	4 cut(s)	95, 306, 760, 854
TscAI	CASTG	3 cut(s)	469, 1117, 1249
TseFI	GTSAC	2 cut(s)	781, 904
TseI	GCWGC	3 cut(s)	344, 767, 1127
Tsp45I	GTSAC	2 cut(s)	781, 904
TspDTI	ATGAA	5 cut(s)	17, 282, 569, 759, 1190
TspGWI	ACGGA	3 cut(s)	381, 417, 600
TspRI	CASTG	3 cut(s)	469, 1117, 1249
VpaK11BI	GGWCC	3 cut(s)	511, 1005, 1195
XapI	RAATTY	1 cut(s)	659
XcmI	CCANNNNNNNNNTGG	2 cut(s)	637, 811
XmaJI	CCTAGG	1 cut(s)	524
XspI	CTAG	3 cut(s)	525, 621, 753
ZraI	GACGTC	1 cut(s)	407
ZrmI	AGTACT	1 cut(s)	677
Zsp2I	ATGCAT	1 cut(s)	297

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.