Prupe.1G435600_v2.0.a1

substituted 4 bases at 4 genomic stop codons

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0022388
Physical Location & Seq
Forward (+)
37191862 .. 37194882
3021 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G435600.3

Sequence Viewer

Length: 1335 bp
ATGAATATAAGATGCCATCCAACCCATCCCATTGCACATACAACTTCGAAAGGCAATATGAATTTTCTCCTTTCATTCTGCTTTTGCCTCTGCGTTTCTCGTTTGCCCTCATTCGCAACTCCAGCTGTGTTATTTCAGGGGTTCAATTGGGATTCAAGTAAAAAAGGAGGATGGTACAACTCCCTCAAGACCACCATTCCCGAACTCGCTAGCTCTGGAATTACCCATGTCTGGCTTCCTCCTCCTTCTCACTCTAATGGAACTGAGGGATACGACCCGGGAAGGCTATACGATCTCGATGCATCAAGCTATGGAAATAAGGACGAATTGAAGTCCCTGATTGCTACTATGCATGAGAAGGGAATCAAAGCCATCGCTGACATTGTGTTAAACCACAGATCAGCTGTCAAACAAGACGAGAATGGAATATGGAGCATCTTTGAAGGTGGAACAGAAGACGGACGTCTTGACTTCAATGCCTCATTGATCTGCAGAGATGACAATGACCATCCTTTTGGAACTGGAAATCCAGACACAGGGGACAACTTTCCTTACACTGCTGATGTGGACCACACCAATCCAAGGCTACAAACCGAGCTCTCTGATTGGTTGAATTGGTTGAAAACTGATATAGGGTTTGATGGATGGAGGTTGGATTATGTGATTGGATATGGCTCCAGCTTCACCAAGCTCTACATGGAAAAGACTTCACCAGAATTTGCAGTTGCTGAGTATTGGCGTTGGAACATTTCAAAAGGGCAGGATGGCAAACTTGACAAGAACCAAGATGCACATAGGAATGAAATAGTGAATTGGATCGAGGCTGCTGGTGGCGTTGTCACGGCGTTCGATTTGACAACCAAGTACATCTTGAATGTTGCTGTGGAGGGTGAGCTATGGAGGCTCAAGGATTCTAATGGAAAACCACCAGGTTTGATTGGGATCAAACCAGAAAGTGCTGTAACATTTATTGACAATCATGACACTTGGTCTCAGGGACTTTTGCCTTTCCCAGATGACAAAGTCCTGCTTGGATATGCTTACATTCTCACCCATCCTGGGACCCCAAGCATATTCTATGATCACTTCTTTGACTGGGGGTGGCCAAAGGAGTCTATACGCAATTTGACTGCAATTAGGGCAAGGAATGGAATAAATTCCAGGAGCAGTGTGACTATTTTAGCTGCAGAGGCTGATCTATATATGGCCAACATTGATGACAAGATTATCATGAAGATTGGGCCAAGATTGGGCCTTGGAGACCTTGATCCCACAAAGTCAAACTTCCATGTTGCCACCAGTGGACAAGACTTCGCTGTTTGGGAGAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase PPR PPR_2

Protein Analysis

445

Amino Acids

49.6

Weight (kDa)

5.37

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022388)

Species Orthologous Gene IDs
malus_domestica MD08G1101700.v1.1
prunus_persica Prupe.1G435600_v2.0.a1
pyrus_communis pycom08g08220 pycom15g07760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 466
AclWI GGATC 3 cut(s) 824, 950, 1262
AcoI YGGCCR 2 cut(s) 1103, 1206
AcsI RAATTY 3 cut(s) 61, 716, 1156
AcyI GRCGYC 1 cut(s) 463
AfaI GTAC 2 cut(s) 176, 866
AfiI CCNNNNNNNGG 5 cut(s) 231, 536, 582, 1059, 1250
AgsI TTSAA 9 cut(s) 145, 156, 331, 443, 475, 613, 622, 753, 874
AhdI GACNNNNNGTC 1 cut(s) 988
AjnI CCWGG 3 cut(s) 928, 1057, 1160
AloI GAACNNNNNNTCC 2 cut(s) 511, 543
AluBI AGCT 9 cut(s) 125, 213, 309, 404, 598, 681, 691, 895, 1184
AluI AGCT 9 cut(s) 125, 213, 309, 404, 598, 681, 691, 895, 1184
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 2 cut(s) 996, 1254
AlwI GGATC 3 cut(s) 824, 950, 1262
AlwNI CAGNNNCTG 2 cut(s) 728, 1193
Ama87I CYCGRG 1 cut(s) 277
AoxI GGCC 4 cut(s) 1103, 1206, 1241, 1252
ApeKI GCWGC 2 cut(s) 824, 1184
ApoI RAATTY 3 cut(s) 61, 716, 1156
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
Asp700I GAANNNNTTC 1 cut(s) 1156
AspS9I GGNCC 4 cut(s) 568, 1062, 1241, 1252
AsuC2I CCSGG 2 cut(s) 278, 279
AsuHPI GGTGA 4 cut(s) 676, 702, 902, 1042
AsuII TTCGAA 1 cut(s) 47
AsuNHI GCTAGC 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 277
AvaII GGWCC 2 cut(s) 568, 1062
BalI TGGCCA 2 cut(s) 1105, 1208
BanII GRGCYC 1 cut(s) 600
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 600
BbvI GCAGC 2 cut(s) 811, 1171
BccI CCATC 9 cut(s) 24, 33, 165, 380, 516, 635, 639, 758, 1062
BceAI ACGGC 1 cut(s) 858
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BciT130I CCWGG 3 cut(s) 930, 1059, 1162
BciVI GTATCC 1 cut(s) 263
BclI TGATCA 1 cut(s) 1081
BcnI CCSGG 2 cut(s) 278, 279
BcoDI GTCTC 2 cut(s) 996, 1254
BfaI CTAG 1 cut(s) 210
BfmI CTRYAG 2 cut(s) 490, 1185
BfuI GTATCC 1 cut(s) 263
BisI GCNGC 2 cut(s) 825, 1185
BlsI GCNGC 2 cut(s) 826, 1186
Bme1390I CCNGG 5 cut(s) 278, 279, 930, 1059, 1162
Bme18I GGWCC 2 cut(s) 568, 1062
BmeRI GACNNNNNGTC 1 cut(s) 988
BmeT110I CYCGRG 1 cut(s) 277
BmgT120I GGNCC 4 cut(s) 568, 1062, 1241, 1252
BmiI GGNNCC 3 cut(s) 676, 1063, 1064
BmrFI CCNGG 5 cut(s) 278, 279, 930, 1059, 1162
BmrI ACTGGG 1 cut(s) 1105
BmsI GCATC 5 cut(s) 2, 289, 311, 444, 778
BmtI GCTAGC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 1105
BoxI GACNNNNGTC 1 cut(s) 462
BpiI GAAGAC 1 cut(s) 462
BpmI CTGGAG 2 cut(s) 105, 661
Bpu14I TTCGAA 1 cut(s) 47
BpuEI CTTGAG 2 cut(s) 170, 890
BpuMI CCSGG 2 cut(s) 278, 279
BsaBI GATNNNNATC 1 cut(s) 941
BsaHI GRCGYC 1 cut(s) 463
BsaI GGTCTC 2 cut(s) 996, 1254
BsaJI CCNNGG 4 cut(s) 277, 581, 1058, 1255
Bsc4I CCNNNNNNNGG 5 cut(s) 231, 536, 582, 1059, 1250
Bse1I ACTGG 3 cut(s) 526, 1100, 1299
Bse3DI GCAATG 1 cut(s) 30
Bse8I GATNNNNATC 1 cut(s) 941
BseBI CCWGG 3 cut(s) 930, 1059, 1162
BseDI CCNNGG 4 cut(s) 277, 581, 1058, 1255
BseGI GGATG 7 cut(s) 16, 25, 176, 508, 650, 769, 1054
BseJI GATNNNNATC 1 cut(s) 941
BseLI CCNNNNNNNGG 5 cut(s) 231, 536, 582, 1059, 1250
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 3 cut(s) 255, 720, 1007
BseNI ACTGG 3 cut(s) 526, 1100, 1299
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 2 cut(s) 811, 1171
BshFI GGCC 4 cut(s) 1105, 1208, 1243, 1254
BsiHKAI GWGCWC 1 cut(s) 600
BsiHKCI CYCGRG 1 cut(s) 277
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 4 cut(s) 319, 554, 1011, 1075
BslI CCNNNNNNNGG 5 cut(s) 231, 536, 582, 1059, 1250
BsmAI GTCTC 2 cut(s) 996, 1254
BsmFI GGGAC 4 cut(s) 319, 554, 1011, 1075
BsnI GGCC 4 cut(s) 1105, 1208, 1243, 1254
Bso31I GGTCTC 2 cut(s) 996, 1254
BsoBI CYCGRG 1 cut(s) 277
Bsp119I TTCGAA 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 8 cut(s) 292, 398, 486, 816, 942, 1081, 1195, 1267
BspANI GGCC 4 cut(s) 1105, 1208, 1243, 1254
BspCNI CTCAG 3 cut(s) 256, 721, 1006
BspHI TCATGA 2 cut(s) 979, 1230
BspLI GGNNCC 3 cut(s) 676, 1063, 1064
BspMAI CTGCAG 2 cut(s) 494, 1189
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 3 cut(s) 824, 950, 1262
BspT104I TTCGAA 1 cut(s) 47
BspTNI GGTCTC 2 cut(s) 996, 1254
BsrDI GCAATG 1 cut(s) 30
BsrI ACTGG 3 cut(s) 526, 1100, 1299
BssECI CCNNGG 4 cut(s) 277, 581, 1058, 1255
BssMI GATC 8 cut(s) 292, 398, 486, 816, 942, 1081, 1195, 1267
BssNI GRCGYC 1 cut(s) 463
BssT1I CCWWGG 2 cut(s) 581, 1255
Bst2UI CCWGG 3 cut(s) 930, 1059, 1162
BstACI GRCGYC 1 cut(s) 463
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 3 cut(s) 264, 729, 993
BstF5I GGATG 7 cut(s) 16, 25, 176, 508, 650, 769, 1054
BstKTI GATC 8 cut(s) 295, 401, 489, 819, 945, 1084, 1198, 1270
BstMAI GTCTC 2 cut(s) 996, 1254
BstMBI GATC 8 cut(s) 292, 398, 486, 816, 942, 1081, 1195, 1267
BstMWI GCNNNNNNNGC 4 cut(s) 122, 901, 1139, 1190
BstNI CCWGG 3 cut(s) 930, 1059, 1162
BstPAI GACNNNNGTC 1 cut(s) 462
BstSCI CCNGG 5 cut(s) 276, 277, 928, 1057, 1160
BstSFI CTRYAG 2 cut(s) 490, 1185
BstV1I GCAGC 2 cut(s) 811, 1171
BstV2I GAAGAC 1 cut(s) 462
BstXI CCANNNNNNTGG 1 cut(s) 515
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 4 cut(s) 1105, 1208, 1243, 1254
BtgZI GCGATG 1 cut(s) 358
BtsCI GGATG 7 cut(s) 16, 25, 176, 508, 650, 769, 1054
BtsI GCAGTG 2 cut(s) 555, 1174
BtsIMutI CAGTG 3 cut(s) 555, 1174, 1306
Cac8I GCNNGC 1 cut(s) 211
CaiI CAGNNNCTG 2 cut(s) 728, 1193
CciI TCATGA 2 cut(s) 979, 1230
Cfr13I GGNCC 4 cut(s) 568, 1062, 1241, 1252
Cfr9I CCCGGG 1 cut(s) 277
CsiI ACCWGGT 1 cut(s) 928
Csp6I GTAC 2 cut(s) 175, 865
CspCI CAANNNNNGTGG 2 cut(s) 915, 950
CviAII CATG 6 cut(s) 227, 353, 697, 980, 1231, 1289
CviQI GTAC 2 cut(s) 175, 865
DdeI CTNAG 3 cut(s) 264, 729, 993
DpnI GATC 8 cut(s) 294, 400, 488, 818, 944, 1083, 1197, 1269
DpnII GATC 8 cut(s) 292, 398, 486, 816, 942, 1081, 1195, 1267
DriI GACNNNNNGTC 1 cut(s) 988
EaeI YGGCCR 2 cut(s) 1103, 1206
Eam1105I GACNNNNNGTC 1 cut(s) 988
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 2 cut(s) 581, 1255
Eco24I GRGCYC 1 cut(s) 600
Eco31I GGTCTC 2 cut(s) 996, 1254
Eco47I GGWCC 2 cut(s) 568, 1062
Eco53kI GAGCTC 1 cut(s) 598
Eco88I CYCGRG 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 1 cut(s) 1062
EcoRII CCWGG 3 cut(s) 928, 1057, 1160
EcoT14I CCWWGG 2 cut(s) 581, 1255
EcoT22I ATGCAT 2 cut(s) 304, 354
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 2 cut(s) 581, 1255
FaeI CATG 6 cut(s) 230, 356, 700, 983, 1234, 1292
FalI AAGNNNNNCTT 6 cut(s) 854, 886, 1014, 1046, 1268, 1300
FaqI GGGAC 4 cut(s) 319, 554, 1011, 1075
FatI CATG 6 cut(s) 226, 352, 696, 979, 1230, 1288
FbaI TGATCA 1 cut(s) 1081
Fnu4HI GCNGC 2 cut(s) 825, 1185
FokI GGATG 7 cut(s) 3, 12, 183, 495, 657, 776, 1041
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 2 cut(s) 825, 1185
FspBI CTAG 1 cut(s) 210
GluI GCNGC 2 cut(s) 825, 1185
GsuI CTGGAG 2 cut(s) 105, 661
HaeIII GGCC 4 cut(s) 1105, 1208, 1243, 1254
HapII CCGG 1 cut(s) 278
Hin1I GRCGYC 1 cut(s) 463
Hin1II CATG 6 cut(s) 230, 356, 700, 983, 1234, 1292
HinfI GANTC 4 cut(s) 152, 363, 911, 1112
HpaII CCGG 1 cut(s) 278
HphI GGTGA 4 cut(s) 676, 702, 902, 1042
Hpy166II GTNNAC 2 cut(s) 568, 1304
Hpy188I TCNGA 1 cut(s) 604
Hpy188III TCNNGA 9 cut(s) 187, 200, 216, 296, 467, 530, 871, 980, 1231
Hpy8I GTNNAC 2 cut(s) 568, 1304
HpyAV CCTTC 4 cut(s) 255, 276, 352, 437
HpyCH4IV ACGT 1 cut(s) 463
HpyCH4V TGCA 8 cut(s) 35, 302, 352, 492, 722, 791, 1133, 1187
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 901, 1139, 1190
HpyF3I CTNAG 3 cut(s) 264, 729, 993
HpySE526I ACGT 1 cut(s) 463
Hsp92I GRCGYC 1 cut(s) 463
Hsp92II CATG 6 cut(s) 230, 356, 700, 983, 1234, 1292
KflI GGGWCCC 1 cut(s) 1062
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 8 cut(s) 292, 398, 486, 816, 942, 1081, 1195, 1267
LmnI GCTCC 3 cut(s) 432, 680, 1164
Lsp1109I GCAGC 2 cut(s) 811, 1171
LweI GCATC 5 cut(s) 2, 289, 311, 444, 778
MabI ACCWGGT 1 cut(s) 928
MaeI CTAG 1 cut(s) 210
MaeII ACGT 1 cut(s) 463
MaeIII GTNAC 3 cut(s) 838, 961, 1171
MalI GATC 8 cut(s) 294, 400, 488, 818, 944, 1083, 1197, 1269
MboI GATC 8 cut(s) 292, 398, 486, 816, 942, 1081, 1195, 1267
MboII GAAGA 2 cut(s) 467, 1246
MfeI CAATTG 1 cut(s) 145
MhlI GDGCHC 1 cut(s) 600
MlsI TGGCCA 2 cut(s) 1105, 1208
MluNI TGGCCA 2 cut(s) 1105, 1208
MlyI GAGTC 1 cut(s) 1121
MmeI TCCRAC 3 cut(s) 44, 633, 722
Mox20I TGGCCA 2 cut(s) 1105, 1208
Mph1103I ATGCAT 2 cut(s) 304, 354
MroXI GAANNNNTTC 1 cut(s) 1156
MscI TGGCCA 2 cut(s) 1105, 1208
MseI TTAA 1 cut(s) 389
MslI CAYNNNNRTG 2 cut(s) 255, 798
Msp20I TGGCCA 2 cut(s) 1105, 1208
MspA1I CMGCKG 2 cut(s) 125, 404
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 5 cut(s) 278, 279, 930, 1059, 1162
MunI CAATTG 1 cut(s) 145
MvaI CCWGG 3 cut(s) 930, 1059, 1162
MwoI GCNNNNNNNGC 4 cut(s) 122, 901, 1139, 1190
NciI CCSGG 2 cut(s) 278, 279
NdeII GATC 8 cut(s) 292, 398, 486, 816, 942, 1081, 1195, 1267
NheI GCTAGC 1 cut(s) 209
NlaIII CATG 6 cut(s) 230, 356, 700, 983, 1234, 1292
NlaIV GGNNCC 3 cut(s) 676, 1063, 1064
NmuCI GTSAC 2 cut(s) 838, 1171
NsiI ATGCAT 2 cut(s) 304, 354
NspV TTCGAA 1 cut(s) 47
PagI TCATGA 2 cut(s) 979, 1230
PdmI GAANNNNTTC 1 cut(s) 1156
PfeI GAWTC 3 cut(s) 152, 363, 911
PflFI GACNNNGTC 1 cut(s) 1022
PfoI TCCNGGA 1 cut(s) 1160
PkrI GCNGC 2 cut(s) 826, 1186
PleI GAGTC 1 cut(s) 1120
PpsI GAGTC 1 cut(s) 1120
PpuMI RGGWCCY 1 cut(s) 1062
PshAI GACNNNNGTC 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 1062
Psp6I CCWGG 3 cut(s) 928, 1057, 1160
PspGI CCWGG 3 cut(s) 928, 1057, 1160
PspN4I GGNNCC 3 cut(s) 676, 1063, 1064
PspPI GGNCC 4 cut(s) 568, 1062, 1241, 1252
PspPPI RGGWCCY 1 cut(s) 1062
PstI CTGCAG 2 cut(s) 494, 1189
PstNI CAGNNNCTG 2 cut(s) 728, 1193
PsyI GACNNNGTC 1 cut(s) 1022
PvuII CAGCTG 2 cut(s) 125, 404
RsaI GTAC 2 cut(s) 176, 866
RsaNI GTAC 2 cut(s) 175, 865
RseI CAYNNNNRTG 2 cut(s) 255, 798
SacI GAGCTC 1 cut(s) 600
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 2 cut(s) 825, 1185
Sau3AI GATC 8 cut(s) 292, 398, 486, 816, 942, 1081, 1195, 1267
Sau96I GGNCC 4 cut(s) 568, 1062, 1241, 1252
SchI GAGTC 1 cut(s) 1121
ScrFI CCNGG 5 cut(s) 278, 279, 930, 1059, 1162
SduI GDGCHC 1 cut(s) 600
SexAI ACCWGGT 1 cut(s) 928
SfaNI GCATC 5 cut(s) 2, 289, 311, 444, 778
SfcI CTRYAG 2 cut(s) 490, 1185
SfuI TTCGAA 1 cut(s) 47
SinI GGWCC 2 cut(s) 568, 1062
SmaI CCCGGG 1 cut(s) 279
SmiMI CAYNNNNRTG 2 cut(s) 255, 798
SmlI CTYRAG 2 cut(s) 185, 905
SmoI CTYRAG 2 cut(s) 185, 905
SspMI CTAG 1 cut(s) 210
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 5 cut(s) 276, 277, 928, 1057, 1160
StyI CCWWGG 2 cut(s) 581, 1255
TaiI ACGT 1 cut(s) 466
TaqI TCGA 4 cut(s) 47, 297, 819, 849
TatI WGTACW 1 cut(s) 864
TfiI GAWTC 3 cut(s) 152, 363, 911
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 3 cut(s) 562, 1174, 1306
TseFI GTSAC 2 cut(s) 838, 1171
TseI GCWGC 2 cut(s) 824, 1184
Tsp45I GTSAC 2 cut(s) 838, 1171
TspDTI ATGAA 5 cut(s) 17, 63, 74, 816, 1247
TspGWI ACGGA 1 cut(s) 474
TspMI CCCGGG 1 cut(s) 277
TspRI CASTG 3 cut(s) 562, 1174, 1306
Tth111I GACNNNGTC 1 cut(s) 1022
VpaK11BI GGWCC 2 cut(s) 568, 1062
XapI RAATTY 3 cut(s) 61, 716, 1156
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XmaI CCCGGG 1 cut(s) 277
XmnI GAANNNNTTC 1 cut(s) 1156
XspI CTAG 1 cut(s) 210
ZraI GACGTC 1 cut(s) 464
Zsp2I ATGCAT 2 cut(s) 304, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.