MD08G1241000.v1.1
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0024392
Physical Location & Seq
Reverse (-)
30684297 .. 30687267
2971 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1241000.v1.1.491

Sequence Viewer

Length: 810 bp
ATGTGGACCCGATCTATCTACCTGCTTATCTGCCTTTCTTTATATACGCGCGTTATTCTTTCATCAGTCATCACCACATACACCAATAACTTAGAGAGAGAGAGAGAGAGAGAGAGAGAGATGAGGAATGCAGGAAGTGATGAGTTTGCTGGTGATGGGGAAATACAGGAGCAGCAGAAGCAGAGGTGGTATTCATCATCTGCATCAAAGTTGGTGCTGCCTCAACTGTGTTTAAATCACGTGTCATTTGTTTGCAAGTCGGTGAGCAAATCAGTGAGGTTCTACGAGGATGTCTTAGGTTTTGTGCTCATCCAACGCCCTTCTTCCTTCAAATTTGAGGGAGCTTGGCTGTTCAACCATGGGGTGGGAATTCATCTGCTGGAGTCGGATACCATCAGTGCCTCGGAAAAGAAATGCAGAAAGATAAACCCAAAGGACAACCATATCTCGTTCCAGTGCTCAGACATGGCGGTGGTGGTGCAGAAACTGGAGGAGATGAAGATAGAGTACGTGACGGCGCAGGTCGAAGAAGGCGGAGTTACGGTTGATCAGCTCTTCTTCCACGACCCTGATAGCTACATGCTTGAAATTTGCAACTGTCATAATCTTCCAGTCCTTCCCATCTCCTCCTCATGCCCTCTCAACCTAATCACCAACACCAACCCAAACCATGCATCGTCGTCTTCCTCATTGTCGTCATCATCGTCGTCCTTCTACGGGAAGCGAAGGTCAGAGAAACCGTGTTCTGGAGAAGCAGCAACGCTGATGATGGAGAGTTTGGTGATGGATGTGATGAACATTTCCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

270

Amino Acids

30.37

Weight (kDa)

5.84

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 78 - 197 1.7e-09 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 79 - 179 2.4e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_2 PF12681 83 - 199 6.4e-07 Glyoxalase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024392)

Species Orthologous Gene IDs
malus_domestica MD08G1241000.v1.1
prunus_persica Prupe.1G577800_v2.0.a1
pyrus_communis pycom08g20890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 30, 511
AccB7I CCANNNNNTGG 1 cut(s) 364
AccII CGCG 2 cut(s) 49, 51
AciI CCGC 2 cut(s) 470, 534
AcsI RAATTY 3 cut(s) 332, 369, 588
AcvI CACGTG 1 cut(s) 241
AfaI GTAC 1 cut(s) 509
AfiI CCNNNNNNNGG 3 cut(s) 364, 717, 746
AflIII ACRYGT 1 cut(s) 240
AgsI TTSAA 3 cut(s) 331, 355, 587
AluBI AGCT 3 cut(s) 344, 553, 576
AluI AGCT 3 cut(s) 344, 553, 576
Alw21I GWGCWC 2 cut(s) 309, 461
AlwNI CAGNNNCTG 1 cut(s) 487
ApeKI GCWGC 3 cut(s) 172, 217, 755
ApoI RAATTY 3 cut(s) 332, 369, 588
AspLEI GCGC 2 cut(s) 51, 520
AspS9I GGNCC 1 cut(s) 6
AsuHPI GGTGA 5 cut(s) 64, 164, 274, 643, 793
AvaII GGWCC 1 cut(s) 6
BarI GAAGNNNNNNTAC 2 cut(s) 491, 523
BbrPI CACGTG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 675
Bbv12I GWGCWC 2 cut(s) 309, 461
BbvI GCAGC 3 cut(s) 184, 204, 767
BccI CCATC 5 cut(s) 149, 401, 629, 763, 778
BceAI ACGGC 1 cut(s) 531
BciVI GTATCC 1 cut(s) 382
BclI TGATCA 1 cut(s) 547
BfuAI ACCTGC 2 cut(s) 30, 511
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 3 cut(s) 173, 218, 756
BlsI GCNGC 3 cut(s) 174, 219, 757
Bme18I GGWCC 1 cut(s) 6
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 1 cut(s) 8
BmsI GCATC 2 cut(s) 212, 683
BpiI GAAGAC 1 cut(s) 675
BpmI CTGGAG 3 cut(s) 401, 509, 768
BsaAI YACGTR 2 cut(s) 241, 511
BsaJI CCNNGG 2 cut(s) 358, 402
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 3 cut(s) 364, 717, 746
Bse1I ACTGG 3 cut(s) 454, 492, 611
BseDI CCNNGG 2 cut(s) 358, 402
BseGI GGATG 3 cut(s) 295, 309, 793
BseLI CCNNNNNNNGG 3 cut(s) 364, 717, 746
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 3 cut(s) 454, 492, 611
BseRI GAGGAG 3 cut(s) 506, 616, 619
BseXI GCAGC 3 cut(s) 184, 204, 767
BsgI GTGCAG 1 cut(s) 500
Bsh1236I CGCG 2 cut(s) 49, 51
BsiHKAI GWGCWC 2 cut(s) 309, 461
BslI CCNNNNNNNGG 3 cut(s) 364, 717, 746
BsmI GAATGC 1 cut(s) 133
Bsp1286I GDGCHC 2 cut(s) 309, 461
Bsp143I GATC 2 cut(s) 11, 547
Bsp19I CCATGG 1 cut(s) 358
BspACI CCGC 2 cut(s) 470, 534
BspCNI CTCAG 1 cut(s) 473
BspFNI CGCG 2 cut(s) 49, 51
BspLI GGNNCC 1 cut(s) 8
BspMI ACCTGC 2 cut(s) 30, 511
BspQI GCTCTTC 1 cut(s) 560
BsrI ACTGG 3 cut(s) 454, 492, 611
BssECI CCNNGG 2 cut(s) 358, 402
BssMI GATC 2 cut(s) 11, 547
BssT1I CCWWGG 1 cut(s) 358
Bst4CI ACNGT 4 cut(s) 228, 544, 599, 741
Bst6I CTCTTC 1 cut(s) 560
BstBAI YACGTR 2 cut(s) 241, 511
BstDEI CTNAG 3 cut(s) 91, 295, 460
BstDSI CCRYGG 1 cut(s) 358
BstF5I GGATG 3 cut(s) 295, 309, 793
BstFNI CGCG 2 cut(s) 49, 51
BstHHI GCGC 2 cut(s) 51, 520
BstKTI GATC 2 cut(s) 14, 550
BstMBI GATC 2 cut(s) 11, 547
BstMWI GCNNNNNNNGC 1 cut(s) 178
BstNSI RCATGY 1 cut(s) 583
BstUI CGCG 2 cut(s) 49, 51
BstV1I GCAGC 3 cut(s) 184, 204, 767
BstV2I GAAGAC 1 cut(s) 675
BsuI GTATCC 1 cut(s) 382
BtgI CCRYGG 1 cut(s) 358
BtsCI GGATG 3 cut(s) 295, 309, 793
BtsIMutI CAGTG 3 cut(s) 279, 403, 461
BveI ACCTGC 2 cut(s) 30, 511
CaiI CAGNNNCTG 1 cut(s) 487
CfoI GCGC 2 cut(s) 51, 520
Cfr13I GGNCC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 508
CviAII CATG 6 cut(s) 359, 466, 580, 633, 671, 805
CviJI RGCY 4 cut(s) 344, 349, 553, 576
CviKI_1 RGCY 4 cut(s) 344, 349, 553, 576
CviQI GTAC 1 cut(s) 508
DdeI CTNAG 3 cut(s) 91, 295, 460
DpnI GATC 2 cut(s) 13, 549
DpnII GATC 2 cut(s) 11, 547
DraI TTTAAA 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 560
EciI GGCGGA 1 cut(s) 549
Eco130I CCWWGG 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 6
Eco72I CACGTG 1 cut(s) 241
EcoRI GAATTC 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 358
EcoT22I ATGCAT 1 cut(s) 676
ErhI CCWWGG 1 cut(s) 358
FaeI CATG 6 cut(s) 362, 469, 583, 636, 674, 808
FatI CATG 6 cut(s) 358, 465, 579, 632, 670, 804
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 173, 218, 756
FokI GGATG 3 cut(s) 296, 302, 800
Fsp4HI GCNGC 3 cut(s) 173, 218, 756
GlaI GCGC 2 cut(s) 50, 519
GluI GCNGC 3 cut(s) 173, 218, 756
GsuI CTGGAG 3 cut(s) 401, 509, 768
HhaI GCGC 2 cut(s) 51, 520
Hin1II CATG 6 cut(s) 362, 469, 583, 636, 674, 808
Hin6I GCGC 2 cut(s) 49, 518
HinP1I GCGC 2 cut(s) 49, 518
HinfI GANTC 1 cut(s) 383
HphI GGTGA 5 cut(s) 64, 164, 274, 643, 793
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 4 cut(s) 388, 406, 463, 733
Hpy188III TCNNGA 1 cut(s) 747
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 2 cut(s) 682, 709
HpyAV CCTTC 6 cut(s) 330, 337, 524, 626, 720, 721
HpyCH4III ACNGT 4 cut(s) 228, 544, 599, 741
HpyCH4IV ACGT 2 cut(s) 240, 510
HpyCH4V TGCA 7 cut(s) 131, 203, 255, 417, 481, 594, 674
HpyF10VI GCNNNNNNNGC 1 cut(s) 178
HpyF3I CTNAG 3 cut(s) 91, 295, 460
HpySE526I ACGT 2 cut(s) 240, 510
Hsp92II CATG 6 cut(s) 362, 469, 583, 636, 674, 808
HspAI GCGC 2 cut(s) 49, 518
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 2 cut(s) 11, 547
LguI GCTCTTC 1 cut(s) 560
LmnI GCTCC 2 cut(s) 169, 341
Lsp1109I GCAGC 3 cut(s) 184, 204, 767
LweI GCATC 2 cut(s) 212, 683
MaeII ACGT 2 cut(s) 240, 510
MaeIII GTNAC 2 cut(s) 511, 538
MalI GATC 2 cut(s) 13, 549
MboI GATC 2 cut(s) 11, 547
MboII GAAGA 7 cut(s) 315, 511, 539, 547, 550, 599, 675
MhlI GDGCHC 2 cut(s) 309, 461
MluCI AATT 3 cut(s) 332, 369, 588
MlyI GAGTC 1 cut(s) 392
MmeI TCCRAC 2 cut(s) 337, 366
Mph1103I ATGCAT 1 cut(s) 676
MseI TTAA 1 cut(s) 233
MslI CAYNNNNRTG 2 cut(s) 470, 803
Mva1269I GAATGC 1 cut(s) 133
MvnI CGCG 2 cut(s) 49, 51
MwoI GCNNNNNNNGC 1 cut(s) 178
NcoI CCATGG 1 cut(s) 358
NdeII GATC 2 cut(s) 11, 547
NlaIII CATG 6 cut(s) 362, 469, 583, 636, 674, 808
NlaIV GGNNCC 1 cut(s) 8
NmuCI GTSAC 1 cut(s) 511
NsiI ATGCAT 1 cut(s) 676
NspI RCATGY 1 cut(s) 583
PciSI GCTCTTC 1 cut(s) 560
PcsI WCGNNNNNNNCGW 1 cut(s) 701
PctI GAATGC 1 cut(s) 133
PflMI CCANNNNNTGG 1 cut(s) 364
PkrI GCNGC 3 cut(s) 174, 219, 757
PleI GAGTC 1 cut(s) 391
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
PpsI GAGTC 1 cut(s) 391
Ppu21I YACGTR 2 cut(s) 241, 511
PspCI CACGTG 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 8
PspPI GGNCC 1 cut(s) 6
PstNI CAGNNNCTG 1 cut(s) 487
RsaI GTAC 1 cut(s) 509
RsaNI GTAC 1 cut(s) 508
RseI CAYNNNNRTG 2 cut(s) 470, 803
SapI GCTCTTC 1 cut(s) 560
SaqAI TTAA 1 cut(s) 233
SatI GCNGC 3 cut(s) 173, 218, 756
Sau3AI GATC 2 cut(s) 11, 547
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 1 cut(s) 392
SduI GDGCHC 2 cut(s) 309, 461
SfaNI GCATC 2 cut(s) 212, 683
SinI GGWCC 1 cut(s) 6
SmiMI CAYNNNNRTG 2 cut(s) 470, 803
Sse9I AATT 3 cut(s) 332, 369, 588
SsiI CCGC 2 cut(s) 470, 534
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 4 cut(s) 228, 544, 599, 741
TaiI ACGT 2 cut(s) 243, 513
TaqI TCGA 1 cut(s) 525
TasI AATT 3 cut(s) 332, 369, 588
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TscAI CASTG 3 cut(s) 279, 403, 461
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 3 cut(s) 172, 217, 755
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 5 cut(s) 51, 183, 362, 512, 809
TspRI CASTG 3 cut(s) 279, 403, 461
Van91I CCANNNNNTGG 1 cut(s) 364
VpaK11BI GGWCC 1 cut(s) 6
XapI RAATTY 3 cut(s) 332, 369, 588
XceI RCATGY 1 cut(s) 583
Zsp2I ATGCAT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.