MD08G1245300.v1.1

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0022334
Physical Location & Seq
Forward (+)
30995424 .. 30996024
601 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1245300.v1.1.491

Sequence Viewer

Length: 276 bp
ATGCAAGCCCTAGAAGATTTTCACTCCGGCAAGGATTCCGGGTCGGATCTCACCAAGCTTCTATCCGACTGCTTCGCCGCATTCAAAGACAATGCTCGGTACAGGGACGACATCAGATTTCTCAAGATTTGGTTCCTCTATATGGATACCAGTGAAGATTTCGAAAGCGTTTTTCTGGAAATGTTGCACGGGGAGATTTGTGTTGGCCACTCTTTGCTTTATATCTGGTACGCGACCTTTCTCGAAGCCAAAGAGAAATTGGACCTCGACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

92

Amino Acids

10.7

Weight (kDa)

4.52

Isoelectric Point (pI)

14.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 9 - 87 4.7e-19 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022334)

Species Orthologous Gene IDs
malus_domestica MD08G1245300.v1.1 MD15G1045400.v1.1
prunus_persica Prupe.3G139700_v2.0.a1 Prupe.3G139700_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 233
AciI CCGC 1 cut(s) 78
AclWI GGATC 1 cut(s) 54
AcoI YGGCCR 1 cut(s) 205
AfaI GTAC 2 cut(s) 101, 230
AfiI CCNNNNNNNGG 1 cut(s) 142
AgsI TTSAA 1 cut(s) 85
AluBI AGCT 1 cut(s) 58
AluI AGCT 1 cut(s) 58
AlwI GGATC 1 cut(s) 54
AoxI GGCC 1 cut(s) 205
Asp700I GAANNNNTTC 1 cut(s) 18
AspS9I GGNCC 1 cut(s) 262
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 43
AsuII TTCGAA 1 cut(s) 162
AvaII GGWCC 1 cut(s) 262
BalI TGGCCA 1 cut(s) 207
BciVI GTATCC 1 cut(s) 139
BcnI CCSGG 1 cut(s) 40
BfaI CTAG 1 cut(s) 11
BfuI GTATCC 1 cut(s) 139
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 40
Bme18I GGWCC 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 40
Bpu14I TTCGAA 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 2 cut(s) 150, 271
BseLI CCNNNNNNNGG 1 cut(s) 142
BseNI ACTGG 2 cut(s) 150, 271
Bsh1236I CGCG 1 cut(s) 233
BshFI GGCC 1 cut(s) 207
BsiSI CCGG 2 cut(s) 27, 39
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 1 cut(s) 207
Bsp119I TTCGAA 1 cut(s) 162
Bsp143I GATC 1 cut(s) 46
BspACI CCGC 1 cut(s) 78
BspANI GGCC 1 cut(s) 207
BspFNI CGCG 1 cut(s) 233
BspLI GGNNCC 1 cut(s) 134
BspPI GGATC 1 cut(s) 54
BspT104I TTCGAA 1 cut(s) 162
BsrI ACTGG 2 cut(s) 150, 271
BssMI GATC 1 cut(s) 46
BstBI TTCGAA 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 6
BstFNI CGCG 1 cut(s) 233
BstKTI GATC 1 cut(s) 49
BstMBI GATC 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 38
BstUI CGCG 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuI GTATCC 1 cut(s) 139
BsuRI GGCC 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 157
Cac8I GCNNGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 262
Csp6I GTAC 2 cut(s) 100, 229
CviJI RGCY 4 cut(s) 8, 58, 207, 248
CviKI_1 RGCY 4 cut(s) 8, 58, 207, 248
CviQI GTAC 2 cut(s) 100, 229
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 262
FaiI YATR 3 cut(s) 141, 143, 222
FaqI GGGAC 1 cut(s) 119
Fnu4HI GCNGC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 1 cut(s) 11
GluI GCNGC 1 cut(s) 78
HaeIII GGCC 1 cut(s) 207
HapII CCGG 2 cut(s) 27, 39
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 1 cut(s) 35
HpaII CCGG 2 cut(s) 27, 39
HphI GGTGA 1 cut(s) 43
Hpy188I TCNGA 3 cut(s) 46, 67, 116
Hpy188III TCNNGA 3 cut(s) 124, 176, 242
HpyCH4V TGCA 2 cut(s) 4, 187
Kzo9I GATC 1 cut(s) 46
LpnPI CCDG 6 cut(s) 40, 52, 88, 161, 163, 211
MaeI CTAG 1 cut(s) 11
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MboII GAAGA 2 cut(s) 26, 167
MflI RGATCY 1 cut(s) 46
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 1 cut(s) 257
MluNI TGGCCA 1 cut(s) 207
MmeI TCCRAC 2 cut(s) 24, 90
MnlI CCTC 2 cut(s) 146, 275
Mox20I TGGCCA 1 cut(s) 207
MroXI GAANNNNTTC 1 cut(s) 18
MscI TGGCCA 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 207
MspI CCGG 2 cut(s) 27, 39
MspR9I CCNGG 1 cut(s) 40
Mva1269I GAATGC 1 cut(s) 80
MvnI CGCG 1 cut(s) 233
NciI CCSGG 1 cut(s) 40
NdeII GATC 1 cut(s) 46
NlaIV GGNNCC 1 cut(s) 134
NspV TTCGAA 1 cut(s) 162
PctI GAATGC 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 134
PspPI GGNCC 1 cut(s) 262
PsuI RGATCY 1 cut(s) 46
RsaI GTAC 2 cut(s) 101, 230
RsaNI GTAC 2 cut(s) 100, 229
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 1 cut(s) 46
Sau96I GGNCC 1 cut(s) 262
ScrFI CCNGG 1 cut(s) 40
SetI ASST 3 cut(s) 60, 239, 267
SfuI TTCGAA 1 cut(s) 162
SinI GGWCC 1 cut(s) 262
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 257
SsiI CCGC 1 cut(s) 78
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 38
TaqI TCGA 3 cut(s) 162, 243, 267
TasI AATT 1 cut(s) 257
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 35
TscAI CASTG 1 cut(s) 157
TspRI CASTG 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.