MD08G1248900.v1.1

peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0022367
Physical Location & Seq
Forward (+)
31343601 .. 31345175
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1248900.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGATGGGATTTTCGGGGTTTGGCTCGGTGGCGTTGAGCTTCTCAGGAGCTGCACGGCTGAGCCAACGCGAAATGGGATTGTTTGGGAATATTAGCATCCATTTTTACCCTGTTGAGAAGAATTTGAAATATAACTATGAGCAAAATTTGGGAAATTTAGATTCTGGGTACAATTATTGGGCAGATTTGATCTTGCCCATTTCGAGAAATCTGTCCTTGAATGATGGGTTGTGGTTTGAAATCCAGAATTCGACCGATACACAGTTGAAGGAATTTAAGATTCCACATAATGCTTATAGGGCTGTGTTGGAGGTATATGTTTCATTTCATGAGAATGATGAGTTTTGGTATTCAAATGCTCCGAATGGGTACATTGCTGCAAACAATCTTAGTGGTACTCCTGGAAAGGGGCCTTTTAGGGAGGTTGTGGTGAGTTTAGATGGTAAGGTTGTTGGTGCAATCCAGCCTTTTACTGTGATTTTCATTGGAGGGTTCAATCCCCTCCTGTGGAGTCCAATTACTGAGATTGGCTCGTACAATCTTCCTTCTTATGATATTGAAATCACGTTCTTTTTAGGGAATATATTGTTAATACTGGATGGGAAGAGCCACAAGTTTGGTTTTAGTGTCACAAATGCCTTAAATGTTTGGTTCGTTGACGCGAATTTGCATATTTGGTTGGACAAGTGGAGCACAAAAACTGAAGGAGAGCTTTCGAAGCATAGTAGCTTGCCCCTTGTTGTTTCATTGGTTTTGGATTTCAAGGGTTTAAATGGCACATTTTTTACAAGGGTCGGCAGGTCTGTGTCATCGACTGGATGGGTGAAGTCCTCCTATGGGAATATCACAACTGATGCAATTCAAGAGCTCCATTATAGTAATACAATGGTCATGAAGAATGGTGGCAATACGCAGATAGTGAATCAGACCATCCATTTCAACGATACAATTCATATCAGGCCGCCATCCTCCATTATCCACTCAATGTCATCAAACAAAAAATTTCCTCTTTACTTGTACATTGACTTTTTGGAACAAGCAAAAGAAACATATAAGTACATTACAAATGTCGAATTGGGATTTCTGTCATGCACAGTATGTGTCGACCGTCTGCCTTGGCTTAGGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

377

Amino Acids

42.43

Weight (kDa)

6.2

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 55 - 241 4e-49 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C PF25156 260 - 337 3.6e-22 PNGase_A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022367)

Species Orthologous Gene IDs
malus_domestica MD08G1248900.v1.1 MD16G1147200.v1.1
pyrus_communis pycom16g12540 pycom16g12550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 789
AccI GTMKAC 1 cut(s) 1104
AccII CGCG 2 cut(s) 69, 662
AciI CCGC 1 cut(s) 962
AcsI RAATTY 7 cut(s) 121, 145, 154, 247, 272, 664, 1001
AcuI CTGAAG 1 cut(s) 723
AfaI GTAC 6 cut(s) 170, 371, 397, 536, 1019, 1058
AfiI CCNNNNNNNGG 3 cut(s) 407, 507, 837
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 5 cut(s) 39, 50, 712, 729, 868
AluI AGCT 5 cut(s) 39, 50, 712, 729, 868
Alw21I GWGCWC 2 cut(s) 695, 870
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 2 cut(s) 410, 959
ApeKI GCWGC 2 cut(s) 50, 377
ApoI RAATTY 7 cut(s) 121, 145, 154, 247, 272, 664, 1001
AspS9I GGNCC 1 cut(s) 410
AsuHPI GGTGA 2 cut(s) 442, 835
AsuII TTCGAA 1 cut(s) 716
BanII GRGCYC 1 cut(s) 870
Bbv12I GWGCWC 2 cut(s) 695, 870
BbvI GCAGC 2 cut(s) 37, 364
BccI CCATC 6 cut(s) 218, 434, 593, 813, 938, 973
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 789
BisI GCNGC 3 cut(s) 51, 378, 962
BlpI GCTNAGC 1 cut(s) 59
BlsI GCNGC 3 cut(s) 52, 379, 963
Bme1390I CCNGG 1 cut(s) 402
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 1 cut(s) 411
BmrFI CCNGG 1 cut(s) 402
BmsI GCATC 2 cut(s) 105, 844
BplI GAGNNNNNCTC 2 cut(s) 515, 547
Bpu10I CCTNAGC 1 cut(s) 1121
Bpu1102I GCTNAGC 1 cut(s) 59
Bpu14I TTCGAA 1 cut(s) 716
BsaJI CCNNGG 1 cut(s) 1115
Bsc4I CCNNNNNNNGG 3 cut(s) 407, 507, 837
Bse1I ACTGG 2 cut(s) 600, 820
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 1115
BseGI GGATG 5 cut(s) 96, 604, 824, 930, 965
BseLI CCNNNNNNNGG 3 cut(s) 407, 507, 837
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 3 cut(s) 50, 57, 513
BseNI ACTGG 2 cut(s) 600, 820
BseXI GCAGC 2 cut(s) 37, 364
BsgI GTGCAG 1 cut(s) 36
Bsh1236I CGCG 2 cut(s) 69, 662
Bsh1285I CGRYCG 2 cut(s) 255, 1108
BshFI GGCC 2 cut(s) 412, 961
BsiEI CGRYCG 2 cut(s) 255, 1108
BsiHKAI GWGCWC 2 cut(s) 695, 870
BslI CCNNNNNNNGG 3 cut(s) 407, 507, 837
BsnI GGCC 2 cut(s) 412, 961
Bsp119I TTCGAA 1 cut(s) 716
Bsp1286I GDGCHC 2 cut(s) 695, 870
Bsp1407I TGTACA 1 cut(s) 1017
Bsp143I GATC 1 cut(s) 189
Bsp1720I GCTNAGC 1 cut(s) 59
BspACI CCGC 1 cut(s) 962
BspANI GGCC 2 cut(s) 412, 961
BspCNI CTCAG 3 cut(s) 51, 56, 514
BspFNI CGCG 2 cut(s) 69, 662
BspHI TCATGA 2 cut(s) 328, 891
BspLI GGNNCC 1 cut(s) 411
BspMI ACCTGC 1 cut(s) 789
BspQI GCTCTTC 1 cut(s) 599
BspT104I TTCGAA 1 cut(s) 716
BsrDI GCAATG 1 cut(s) 372
BsrGI TGTACA 1 cut(s) 1017
BsrI ACTGG 2 cut(s) 600, 820
BssECI CCNNGG 1 cut(s) 1115
BssMI GATC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 1115
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 4 cut(s) 264, 475, 1096, 1109
Bst6I CTCTTC 1 cut(s) 599
BstAUI TGTACA 1 cut(s) 1017
BstBI TTCGAA 1 cut(s) 716
BstC8I GCNNGC 1 cut(s) 731
BstDEI CTNAG 5 cut(s) 43, 59, 389, 522, 1121
BstF5I GGATG 5 cut(s) 96, 604, 824, 930, 965
BstFNI CGCG 2 cut(s) 69, 662
BstKTI GATC 1 cut(s) 192
BstMBI GATC 1 cut(s) 189
BstMCI CGRYCG 2 cut(s) 255, 1108
BstMWI GCNNNNNNNGC 2 cut(s) 299, 718
BstNI CCWGG 1 cut(s) 402
BstSCI CCNGG 1 cut(s) 400
BstUI CGCG 2 cut(s) 69, 662
BstV1I GCAGC 2 cut(s) 37, 364
BstXI CCANNNNNNTGG 1 cut(s) 617
BsuRI GGCC 2 cut(s) 412, 961
BtsCI GGATG 5 cut(s) 96, 604, 824, 930, 965
BveI ACCTGC 1 cut(s) 789
Cac8I GCNNGC 1 cut(s) 731
CaiI CAGNNNCTG 1 cut(s) 50
CciI TCATGA 2 cut(s) 328, 891
Cfr13I GGNCC 1 cut(s) 410
CseI GACGC 1 cut(s) 668
Csp6I GTAC 6 cut(s) 169, 370, 396, 535, 1018, 1057
CspCI CAANNNNNGTGG 2 cut(s) 373, 408
CviAII CATG 3 cut(s) 329, 892, 1089
CviQI GTAC 6 cut(s) 169, 370, 396, 535, 1018, 1057
DdeI CTNAG 5 cut(s) 43, 59, 389, 522, 1121
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
DraI TTTAAA 1 cut(s) 771
Eam1104I CTCTTC 1 cut(s) 599
EarI CTCTTC 1 cut(s) 599
Ecl136II GAGCTC 1 cut(s) 868
Eco130I CCWWGG 1 cut(s) 1115
Eco24I GRGCYC 1 cut(s) 870
Eco53kI GAGCTC 1 cut(s) 868
Eco57I CTGAAG 1 cut(s) 723
EcoICRI GAGCTC 1 cut(s) 868
EcoO109I RGGNCCY 1 cut(s) 410
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 1115
EcoT38I GRGCYC 1 cut(s) 870
ErhI CCWWGG 1 cut(s) 1115
FaeI CATG 3 cut(s) 332, 895, 1092
FalI AAGNNNNNCTT 2 cut(s) 696, 728
FatI CATG 3 cut(s) 328, 891, 1088
FblI GTMKAC 1 cut(s) 1104
Fnu4HI GCNGC 3 cut(s) 51, 378, 962
FokI GGATG 5 cut(s) 83, 611, 831, 917, 952
FriOI GRGCYC 1 cut(s) 870
Fsp4HI GCNGC 3 cut(s) 51, 378, 962
GluI GCNGC 3 cut(s) 51, 378, 962
HaeIII GGCC 2 cut(s) 412, 961
HgaI GACGC 1 cut(s) 668
Hin1II CATG 3 cut(s) 332, 895, 1092
HincII GTYRAC 2 cut(s) 658, 1105
HindII GTYRAC 2 cut(s) 658, 1105
HinfI GANTC 4 cut(s) 161, 280, 511, 922
HphI GGTGA 2 cut(s) 442, 835
Hpy166II GTNNAC 2 cut(s) 658, 1105
Hpy188I TCNGA 2 cut(s) 363, 927
Hpy188III TCNNGA 6 cut(s) 45, 204, 244, 329, 863, 892
Hpy8I GTNNAC 2 cut(s) 658, 1105
HpyAV CCTTC 3 cut(s) 262, 555, 698
HpyCH4III ACNGT 4 cut(s) 264, 475, 1096, 1109
HpyCH4IV ACGT 1 cut(s) 566
HpyCH4V TGCA 6 cut(s) 53, 380, 458, 670, 857, 1092
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 718
HpyF3I CTNAG 5 cut(s) 43, 59, 389, 522, 1121
HpySE526I ACGT 1 cut(s) 566
Hsp92II CATG 3 cut(s) 332, 895, 1092
Kzo9I GATC 1 cut(s) 189
LguI GCTCTTC 1 cut(s) 599
LmnI GCTCC 4 cut(s) 47, 364, 690, 873
Lsp1109I GCAGC 2 cut(s) 37, 364
LweI GCATC 2 cut(s) 105, 844
MaeII ACGT 1 cut(s) 566
MaeIII GTNAC 1 cut(s) 628
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 4 cut(s) 130, 533, 616, 907
MhlI GDGCHC 2 cut(s) 695, 870
MlyI GAGTC 1 cut(s) 520
MmeI TCCRAC 2 cut(s) 288, 660
MnlI CCTC 7 cut(s) 304, 415, 482, 512, 841, 979, 1017
MseI TTAA 4 cut(s) 276, 590, 641, 770
MslI CAYNNNNRTG 1 cut(s) 333
MspR9I CCNGG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 2 cut(s) 69, 662
MwoI GCNNNNNNNGC 2 cut(s) 299, 718
NdeII GATC 1 cut(s) 189
NlaIII CATG 3 cut(s) 332, 895, 1092
NlaIV GGNNCC 1 cut(s) 411
NmuCI GTSAC 1 cut(s) 628
NspV TTCGAA 1 cut(s) 716
PagI TCATGA 2 cut(s) 328, 891
PciSI GCTCTTC 1 cut(s) 599
PfeI GAWTC 3 cut(s) 161, 280, 922
PfoI TCCNGGA 1 cut(s) 400
PkrI GCNGC 3 cut(s) 52, 379, 963
PleI GAGTC 1 cut(s) 519
PpsI GAGTC 1 cut(s) 519
Psp124BI GAGCTC 1 cut(s) 870
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 411
PspPI GGNCC 1 cut(s) 410
PstNI CAGNNNCTG 1 cut(s) 50
RsaI GTAC 6 cut(s) 170, 371, 397, 536, 1019, 1058
RsaNI GTAC 6 cut(s) 169, 370, 396, 535, 1018, 1057
RseI CAYNNNNRTG 1 cut(s) 333
SacI GAGCTC 1 cut(s) 870
SalI GTCGAC 1 cut(s) 1103
SapI GCTCTTC 1 cut(s) 599
SaqAI TTAA 4 cut(s) 276, 590, 641, 770
SatI GCNGC 3 cut(s) 51, 378, 962
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 1 cut(s) 520
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 2 cut(s) 695, 870
SfaNI GCATC 2 cut(s) 105, 844
SfuI TTCGAA 1 cut(s) 716
SmiMI CAYNNNNRTG 1 cut(s) 333
SsiI CCGC 1 cut(s) 962
SspI AATATT 1 cut(s) 91
SstI GAGCTC 1 cut(s) 870
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 1 cut(s) 1115
TaaI ACNGT 4 cut(s) 264, 475, 1096, 1109
TaiI ACGT 1 cut(s) 569
TaqI TCGA 6 cut(s) 203, 251, 716, 812, 1071, 1104
TaqII GACCGA 1 cut(s) 269
TatI WGTACW 2 cut(s) 1017, 1056
TauI GCSGC 1 cut(s) 964
TfiI GAWTC 3 cut(s) 161, 280, 922
Tru1I TTAA 4 cut(s) 276, 590, 641, 770
Tru9I TTAA 4 cut(s) 276, 590, 641, 770
TseFI GTSAC 1 cut(s) 628
TseI GCWGC 2 cut(s) 50, 377
Tsp45I GTSAC 1 cut(s) 628
TspDTI ATGAA 6 cut(s) 312, 317, 472, 735, 908, 941
XapI RAATTY 7 cut(s) 121, 145, 154, 247, 272, 664, 1001
XmiI GTMKAC 1 cut(s) 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.