MD16G1147200.v1.1

peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0022367
Physical Location & Seq
Forward (+)
11497403 .. 11498624
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1147200.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGATCGGATTTTCGGGGTTTGGCTCGGCGAGGTTGAGCTTCTCAAGAGCTGCATCGCTGAGCCAACACAGAATGGGATTGTTTGGAGTGTCAAGAAAGACATCACAAGATAAAACATACATAGGGATTTACCATGTGAATATTAGCATCCATTTTTACCCTGCTGAGAAGAATTTGAATTATAATTATGAGCAAAATTTGGGAAATTTGGATTCTGGGTACCATTATTGGGCGGATTTGATCTTGCCCATTTCGAGAAATCTGCCTTTGAATGATGGGATGTGGTTTGAAATCCAGAATTCGACCAATACACAGTTGAAGGAATTTAAGATTCCACAAAACGCTTATAGGGCTGTGTTGGAGGTATATGTTTCATTTCATGAGAATGATGAGTTTTGGTATTCAAATGCTCCGAATGAGTACATTGTTGTAAACAATCTTAGAGGTATGCCTGGAAACAGGCCTTTTAGGGAGGTTGGGGTGAGTTTAGATGGTAAGGTTGTTGGTGCAATCTATCCTTTTACAGTGATTTTCACTGGAGGGTTCAATCCCCTCCTATGGAGTTCAATTACTGCGATTGGCTCGTACAATCTCCCTTCCTATGATATTGAAATCACGCCCTTTTTAGGGAAGGTATTGGATGGGAAGAGCCACAAGTTTGGTTTTAGTGTCACAAATGCATTGAATGTTTGGTTTGTTGATGCGAATTTGCATCTTTGGTTGGACAAGCAGAGCACAAAAACTGAAGGAGAGCTTTCGGAGCATAGTAGCTTGCCCCTTGTTGTTTCATTGGTTTTGGATTTCAAGGGTTTAAATGGCACATTTTTTACAAAGGGCGGCAGGTCTGTGTCATCGACTGGATGGGTAAAGTCCTCCTATGGGAATATCACAACTGATGCAATTCAAGACCTCCATTACAGTAATACAATGGTCATGAACAATGATGGCAATACGCAAATAGTGAATCAGACAATCCATTTCAACAATACAGTTCATATCAGGCCGCCATCCTTCATTATCCACTCAATGTCATCATACAAAAAATTTCCTCTTTACTTGTACATTGACTTCTTGGAACAAGCAAAAGAAACATATAAGTACGTTACAAATGTCGAATTGGGATTTCTGTTATGCATAGTATATGTCGACCGTCTGCCTTGGCTTAGGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

391

Amino Acids

44.4

Weight (kDa)

6.87

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 36 - 255 7e-56 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C PF25156 274 - 351 1.8e-20 PNGase_A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022367)

Species Orthologous Gene IDs
malus_domestica MD08G1248900.v1.1 MD16G1147200.v1.1
pyrus_communis pycom16g12540 pycom16g12550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 183
Acc36I ACCTGC 1 cut(s) 831
Acc65I GGTACC 1 cut(s) 219
AccB1I GGYRCC 1 cut(s) 219
AccI GTMKAC 1 cut(s) 1146
AciI CCGC 3 cut(s) 233, 837, 1004
AcsI RAATTY 7 cut(s) 172, 196, 205, 298, 323, 706, 1043
AcuI CTGAAG 1 cut(s) 765
AfaI GTAC 5 cut(s) 221, 422, 587, 1061, 1100
AfiI CCNNNNNNNGG 5 cut(s) 229, 558, 626, 627, 879
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 4 cut(s) 39, 50, 754, 771
AluI AGCT 4 cut(s) 39, 50, 754, 771
Alw21I GWGCWC 1 cut(s) 737
AoxI GGCC 2 cut(s) 461, 1001
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 7 cut(s) 172, 196, 205, 298, 323, 706, 1043
Asp718I GGTACC 1 cut(s) 219
AsuHPI GGTGA 1 cut(s) 493
BanI GGYRCC 1 cut(s) 219
BarI GAAGNNNNNNTAC 2 cut(s) 1052, 1084
Bbv12I GWGCWC 1 cut(s) 737
BbvI GCAGC 1 cut(s) 37
BccI CCATC 6 cut(s) 269, 485, 635, 855, 938, 1015
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 1 cut(s) 453
BfuAI ACCTGC 1 cut(s) 831
BisI GCNGC 3 cut(s) 51, 838, 1004
BlpI GCTNAGC 1 cut(s) 59
BlsI GCNGC 3 cut(s) 52, 839, 1005
Bme1390I CCNGG 1 cut(s) 453
BmiI GGNNCC 1 cut(s) 221
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 5 cut(s) 62, 156, 691, 721, 886
BpmI CTGGAG 1 cut(s) 558
Bpu10I CCTNAGC 1 cut(s) 1163
Bpu1102I GCTNAGC 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 1157
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 558, 626, 627, 879
Bse1I ACTGG 2 cut(s) 541, 862
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 1 cut(s) 1157
BseGI GGATG 5 cut(s) 147, 285, 646, 866, 1007
BseLI CCNNNNNNNGG 5 cut(s) 229, 558, 626, 627, 879
BseMII CTCAG 2 cut(s) 50, 156
BseNI ACTGG 2 cut(s) 541, 862
BseXI GCAGC 1 cut(s) 37
Bsh1285I CGRYCG 1 cut(s) 1150
BshFI GGCC 2 cut(s) 463, 1003
BshNI GGYRCC 1 cut(s) 219
BsiEI CGRYCG 1 cut(s) 1150
BsiHKAI GWGCWC 1 cut(s) 737
BslI CCNNNNNNNGG 5 cut(s) 229, 558, 626, 627, 879
BsnI GGCC 2 cut(s) 463, 1003
Bsp1286I GDGCHC 1 cut(s) 737
Bsp1407I TGTACA 1 cut(s) 1059
Bsp143I GATC 2 cut(s) 3, 240
Bsp1720I GCTNAGC 1 cut(s) 59
BspACI CCGC 3 cut(s) 233, 837, 1004
BspANI GGCC 2 cut(s) 463, 1003
BspCNI CTCAG 2 cut(s) 51, 157
BspHI TCATGA 2 cut(s) 379, 933
BspLI GGNNCC 1 cut(s) 221
BspMI ACCTGC 1 cut(s) 831
BspQI GCTCTTC 1 cut(s) 641
BspT107I GGYRCC 1 cut(s) 219
BsrGI TGTACA 1 cut(s) 1059
BsrI ACTGG 2 cut(s) 541, 862
BssECI CCNNGG 1 cut(s) 1157
BssMI GATC 2 cut(s) 3, 240
BssT1I CCWWGG 1 cut(s) 1157
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 5 cut(s) 315, 526, 920, 991, 1151
Bst6I CTCTTC 1 cut(s) 641
BstAUI TGTACA 1 cut(s) 1059
BstC8I GCNNGC 1 cut(s) 773
BstDEI CTNAG 4 cut(s) 59, 165, 440, 1163
BstF5I GGATG 5 cut(s) 147, 285, 646, 866, 1007
BstKTI GATC 2 cut(s) 6, 243
BstMBI GATC 2 cut(s) 3, 240
BstMCI CGRYCG 1 cut(s) 1150
BstMWI GCNNNNNNNGC 2 cut(s) 350, 760
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 37
BstXI CCANNNNNNTGG 1 cut(s) 659
BsuRI GGCC 2 cut(s) 463, 1003
BtgZI GCGATG 1 cut(s) 39
BtsCI GGATG 5 cut(s) 147, 285, 646, 866, 1007
BtsIMutI CAGTG 2 cut(s) 531, 534
BveI ACCTGC 1 cut(s) 831
Cac8I GCNNGC 1 cut(s) 773
CciI TCATGA 2 cut(s) 379, 933
Csp6I GTAC 5 cut(s) 220, 421, 586, 1060, 1099
CviAII CATG 3 cut(s) 134, 380, 934
CviQI GTAC 5 cut(s) 220, 421, 586, 1060, 1099
DdeI CTNAG 4 cut(s) 59, 165, 440, 1163
DpnI GATC 2 cut(s) 5, 242
DpnII GATC 2 cut(s) 3, 240
DraI TTTAAA 1 cut(s) 813
Eam1104I CTCTTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 641
EciI GGCGGA 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 1157
Eco147I AGGCCT 1 cut(s) 463
Eco57I CTGAAG 1 cut(s) 765
EcoRI GAATTC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 1157
EcoT22I ATGCAT 2 cut(s) 682, 1136
ErhI CCWWGG 1 cut(s) 1157
FaeI CATG 3 cut(s) 137, 383, 937
FalI AAGNNNNNCTT 2 cut(s) 738, 770
FatI CATG 3 cut(s) 133, 379, 933
FblI GTMKAC 1 cut(s) 1146
Fnu4HI GCNGC 3 cut(s) 51, 838, 1004
FokI GGATG 5 cut(s) 134, 292, 653, 873, 994
Fsp4HI GCNGC 3 cut(s) 51, 838, 1004
GluI GCNGC 3 cut(s) 51, 838, 1004
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 2 cut(s) 463, 1003
Hin1II CATG 3 cut(s) 137, 383, 937
HincII GTYRAC 1 cut(s) 1147
HindII GTYRAC 1 cut(s) 1147
HinfI GANTC 3 cut(s) 212, 331, 964
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 2 cut(s) 433, 1147
Hpy188I TCNGA 4 cut(s) 8, 414, 760, 969
Hpy188III TCNNGA 7 cut(s) 45, 93, 255, 295, 380, 905, 934
Hpy8I GTNNAC 2 cut(s) 433, 1147
HpyAV CCTTC 5 cut(s) 313, 606, 625, 740, 1021
HpyCH4III ACNGT 5 cut(s) 315, 526, 920, 991, 1151
HpyCH4IV ACGT 1 cut(s) 1101
HpyCH4V TGCA 6 cut(s) 53, 509, 680, 712, 899, 1134
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 760
HpyF3I CTNAG 4 cut(s) 59, 165, 440, 1163
HpySE526I ACGT 1 cut(s) 1101
Hsp92II CATG 3 cut(s) 137, 383, 937
KpnI GGTACC 1 cut(s) 223
Kzo9I GATC 2 cut(s) 3, 240
LguI GCTCTTC 1 cut(s) 641
LmnI GCTCC 2 cut(s) 415, 760
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 5 cut(s) 62, 156, 691, 721, 886
MaeII ACGT 1 cut(s) 1101
MaeIII GTNAC 2 cut(s) 670, 1102
MalI GATC 2 cut(s) 5, 242
MboI GATC 2 cut(s) 3, 240
MboII GAAGA 2 cut(s) 181, 658
MhlI GDGCHC 1 cut(s) 737
MmeI TCCRAC 2 cut(s) 339, 702
MnlI CCTC 9 cut(s) 24, 355, 437, 466, 533, 563, 883, 920, 1059
Mph1103I ATGCAT 2 cut(s) 682, 1136
MseI TTAA 2 cut(s) 327, 812
MslI CAYNNNNRTG 1 cut(s) 384
MspR9I CCNGG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 453
MwoI GCNNNNNNNGC 2 cut(s) 350, 760
NdeII GATC 2 cut(s) 3, 240
NlaIII CATG 3 cut(s) 137, 383, 937
NlaIV GGNNCC 1 cut(s) 221
NmeAIII GCCGAG 1 cut(s) 5
NmuCI GTSAC 1 cut(s) 670
NsiI ATGCAT 2 cut(s) 682, 1136
PagI TCATGA 2 cut(s) 379, 933
PceI AGGCCT 1 cut(s) 463
PciSI GCTCTTC 1 cut(s) 641
PfeI GAWTC 3 cut(s) 212, 331, 964
PkrI GCNGC 3 cut(s) 52, 839, 1005
PsiI TTATAA 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 221
RsaI GTAC 5 cut(s) 221, 422, 587, 1061, 1100
RsaNI GTAC 5 cut(s) 220, 421, 586, 1060, 1099
RseI CAYNNNNRTG 1 cut(s) 384
SalI GTCGAC 1 cut(s) 1145
SapI GCTCTTC 1 cut(s) 641
SaqAI TTAA 2 cut(s) 327, 812
SatI GCNGC 3 cut(s) 51, 838, 1004
Sau3AI GATC 2 cut(s) 3, 240
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 1 cut(s) 737
SfaNI GCATC 5 cut(s) 62, 156, 691, 721, 886
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 1 cut(s) 43
SmoI CTYRAG 1 cut(s) 43
SseBI AGGCCT 1 cut(s) 463
SsiI CCGC 3 cut(s) 233, 837, 1004
SspI AATATT 1 cut(s) 142
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 1 cut(s) 451
StyI CCWWGG 1 cut(s) 1157
TaaI ACNGT 5 cut(s) 315, 526, 920, 991, 1151
TaiI ACGT 1 cut(s) 1104
TaqI TCGA 5 cut(s) 254, 302, 854, 1113, 1146
TatI WGTACW 2 cut(s) 420, 1059
TauI GCSGC 2 cut(s) 840, 1006
TfiI GAWTC 3 cut(s) 212, 331, 964
Tru1I TTAA 2 cut(s) 327, 812
Tru9I TTAA 2 cut(s) 327, 812
TscAI CASTG 2 cut(s) 531, 541
TseFI GTSAC 1 cut(s) 670
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 670
TspDTI ATGAA 6 cut(s) 363, 368, 777, 950, 983, 1003
TspRI CASTG 2 cut(s) 531, 541
XapI RAATTY 7 cut(s) 172, 196, 205, 298, 323, 706, 1043
XmiI GTMKAC 1 cut(s) 1146
Zsp2I ATGCAT 2 cut(s) 682, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.