MD09G1061300.v1.1

Domain of unknown function (DUF4336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0022347
Physical Location & Seq
Forward (+)
4188730 .. 4190603
1874 bp
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UTR
Exon/CDS
Intron
MD09G1061300.v1.1.491

Sequence Viewer

Length: 771 bp
ATGGGTGGCACCAAGGCAATGGGTGCCAAGACCTTAAAGGATGAGGACTTCTCCACCTCAAGGGCTGATGAGATTGAGCAGAAAGTTCTCAGTTCACCAGAAGTTGATGCTGTCATTTTTGTGCCAAGACAGCCGCCTGATTGTATCAGCAAAGAATCCTTGTTGGCATCTGCGGTGAATGGTTTGGCGGTAAAACTTATCAGTAAAGGAAAAGAAGTATCTCAAGAACCAGTAGTTGACAACAAGTTGAACCGTCAAAAAGGATGGGAAAGAATGGTTCTTCAAATCTTGTTTCTCAGTCCATCAAATCTGTTGGAACCGAATGCTAGCTTTGCTCAGATGTCACAAAAACTGATTGTTTCACCCATTGTAAAGACCCTGGTCTTCAGCAAAGTCAGTGATTGGGTTGATGGAATCGCAAAGGACTGGAGTTTCAGGAGAACAATCCCCGCTCATTTTGCTGCACCAATCAATGCAAGCAGGTCTGATCTGTTAGCTGCATTTGCCTTTCTGGATGACCTTCTGGATGAACGTTATGTCACTAGGCCTTCACTCTCTCTTCTCTTCACATCACTCTTGGGAAAGGCCGCAAGCTACTTCCCTCCCGATGACATGCAGACTTTATCATCCCTCGGTCAGTTTTTGGTCTCGATAGGAGCTGTGAAAAAGACGGTCTGCTTAGGGTACAAAGGTTCTTTGGCCAAAAGAAAAGGTAAACTATTTAACATCCTAAAGTTGTACAAAAGCAAAAGGTGCAATACAAGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4336

Protein Analysis

257

Amino Acids

28.17

Weight (kDa)

9.5

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4336 PF14234 36 - 104 1.7e-08 Domain of unknown function (DUF4336)
DUF4336 PF14234 108 - 170 2.1e-13 Domain of unknown function (DUF4336)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022347)

Species Orthologous Gene IDs
malus_domestica MD09G1061300.v1.1
prunus_persica Prupe.3G258900_v2.0.a1
pyrus_communis pycom111g05180
rosa_multiflora Rmu_co8296441.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 471
AccB1I GGYRCC 2 cut(s) 8, 23
AccBSI CCGCTC 1 cut(s) 452
AciI CCGC 5 cut(s) 134, 173, 188, 450, 588
AclI AACGTT 1 cut(s) 532
AcoI YGGCCR 1 cut(s) 699
AcuI CTGAAG 1 cut(s) 370
AfaI GTAC 2 cut(s) 686, 740
AfiI CCNNNNNNNGG 1 cut(s) 60
AgsI TTSAA 2 cut(s) 250, 284
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 5 cut(s) 330, 497, 594, 659, 765
AluI AGCT 5 cut(s) 330, 497, 594, 659, 765
Alw26I GTCTC 1 cut(s) 652
AoxI GGCC 3 cut(s) 545, 585, 699
ApeKI GCWGC 2 cut(s) 461, 497
AsuHPI GGTGA 3 cut(s) 87, 187, 354
AsuNHI GCTAGC 1 cut(s) 326
BaeI ACNNNNGTAYC 2 cut(s) 676, 709
BalI TGGCCA 1 cut(s) 701
BanI GGYRCC 2 cut(s) 8, 23
BbsI GAAGAC 1 cut(s) 376
BbvI GCAGC 2 cut(s) 448, 484
BccI CCATC 3 cut(s) 258, 310, 404
BciT130I CCWGG 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 652
BfaI CTAG 3 cut(s) 327, 543, 769
BfuAI ACCTGC 1 cut(s) 471
BisI GCNGC 4 cut(s) 134, 462, 498, 588
BlsI GCNGC 4 cut(s) 135, 463, 499, 589
Bme1390I CCNGG 1 cut(s) 380
BmiI GGNNCC 3 cut(s) 10, 25, 318
BmrFI CCNGG 1 cut(s) 380
BmsI GCATC 2 cut(s) 97, 176
BmtI GCTAGC 1 cut(s) 330
BoxI GACNNNNGTC 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 376
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpmI CTGGAG 1 cut(s) 448
Bpu10I CCTNAGC 1 cut(s) 679
BpuEI CTTGAG 2 cut(s) 43, 207
BsaI GGTCTC 1 cut(s) 652
BsaJI CCNNGG 3 cut(s) 12, 378, 631
Bsc4I CCNNNNNNNGG 1 cut(s) 60
Bse1I ACTGG 2 cut(s) 230, 431
Bse3DI GCAATG 1 cut(s) 24
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 3 cut(s) 12, 378, 631
BseGI GGATG 6 cut(s) 46, 269, 520, 532, 626, 726
BseLI CCNNNNNNNGG 1 cut(s) 60
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 3 cut(s) 103, 310, 350
BseNI ACTGG 2 cut(s) 230, 431
BseXI GCAGC 2 cut(s) 448, 484
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 3 cut(s) 547, 587, 701
BshNI GGYRCC 2 cut(s) 8, 23
BslI CCNNNNNNNGG 1 cut(s) 60
BsmAI GTCTC 1 cut(s) 652
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 3 cut(s) 547, 587, 701
Bso31I GGTCTC 1 cut(s) 652
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 1 cut(s) 487
BspACI CCGC 5 cut(s) 134, 173, 188, 450, 588
BspANI GGCC 3 cut(s) 547, 587, 701
BspCNI CTCAG 3 cut(s) 102, 309, 349
BspLI GGNNCC 3 cut(s) 10, 25, 318
BspMI ACCTGC 1 cut(s) 471
BspOI GCTAGC 1 cut(s) 330
BspT107I GGYRCC 2 cut(s) 8, 23
BspTNI GGTCTC 1 cut(s) 652
BsrBI CCGCTC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 24
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 2 cut(s) 230, 431
BssECI CCNNGG 3 cut(s) 12, 378, 631
BssMI GATC 1 cut(s) 487
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 2 cut(s) 254, 673
Bst6I CTCTTC 2 cut(s) 564, 569
BstAPI GCANNNNNTGC 2 cut(s) 23, 753
BstAUI TGTACA 1 cut(s) 738
BstC8I GCNNGC 3 cut(s) 328, 478, 592
BstDEI CTNAG 4 cut(s) 89, 296, 336, 679
BstF5I GGATG 6 cut(s) 46, 269, 520, 532, 626, 726
BstKTI GATC 1 cut(s) 490
BstMAI GTCTC 1 cut(s) 652
BstMBI GATC 1 cut(s) 487
BstMWI GCNNNNNNNGC 7 cut(s) 23, 130, 332, 458, 503, 753, 762
BstNI CCWGG 1 cut(s) 380
BstNSI RCATGY 1 cut(s) 616
BstPAI GACNNNNGTC 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 378
BstV1I GCAGC 2 cut(s) 448, 484
BstV2I GAAGAC 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 19
BsuRI GGCC 3 cut(s) 547, 587, 701
BtsCI GGATG 6 cut(s) 46, 269, 520, 532, 626, 726
BtsIMutI CAGTG 1 cut(s) 403
BveI ACCTGC 1 cut(s) 471
Cac8I GCNNGC 3 cut(s) 328, 478, 592
Csp6I GTAC 2 cut(s) 685, 739
CviAII CATG 1 cut(s) 613
CviQI GTAC 2 cut(s) 685, 739
DdeI CTNAG 4 cut(s) 89, 296, 336, 679
DpnI GATC 1 cut(s) 489
DpnII GATC 1 cut(s) 487
EaeI YGGCCR 1 cut(s) 699
Eam1104I CTCTTC 2 cut(s) 564, 569
EarI CTCTTC 2 cut(s) 564, 569
Eco130I CCWWGG 1 cut(s) 12
Eco147I AGGCCT 1 cut(s) 547
Eco31I GGTCTC 1 cut(s) 652
Eco57I CTGAAG 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 1 cut(s) 616
FaiI YATR 2 cut(s) 537, 614
FatI CATG 1 cut(s) 612
FauI CCCGC 1 cut(s) 457
Fnu4HI GCNGC 4 cut(s) 134, 462, 498, 588
FokI GGATG 6 cut(s) 53, 276, 527, 539, 613, 713
Fsp4HI GCNGC 4 cut(s) 134, 462, 498, 588
FspBI CTAG 3 cut(s) 327, 543, 769
GluI GCNGC 4 cut(s) 134, 462, 498, 588
GsuI CTGGAG 1 cut(s) 448
HaeIII GGCC 3 cut(s) 547, 587, 701
Hin1II CATG 1 cut(s) 616
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HindIII AAGCTT 1 cut(s) 763
HinfI GANTC 2 cut(s) 155, 414
HphI GGTGA 3 cut(s) 87, 187, 354
Hpy166II GTNNAC 3 cut(s) 95, 238, 716
Hpy188I TCNGA 2 cut(s) 339, 487
Hpy188III TCNNGA 6 cut(s) 224, 436, 512, 524, 605, 649
Hpy8I GTNNAC 3 cut(s) 95, 238, 716
HpyAV CCTTC 2 cut(s) 530, 558
HpyCH4III ACNGT 2 cut(s) 254, 673
HpyCH4IV ACGT 1 cut(s) 532
HpyCH4V TGCA 5 cut(s) 464, 476, 500, 616, 756
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 130, 332, 458, 503, 753, 762
HpyF3I CTNAG 4 cut(s) 89, 296, 336, 679
HpySE526I ACGT 1 cut(s) 532
Hsp92II CATG 1 cut(s) 616
Kzo9I GATC 1 cut(s) 487
LmnI GCTCC 1 cut(s) 656
Lsp1109I GCAGC 2 cut(s) 448, 484
LweI GCATC 2 cut(s) 97, 176
MaeI CTAG 3 cut(s) 327, 543, 769
MaeII ACGT 1 cut(s) 532
MaeIII GTNAC 2 cut(s) 342, 538
MalI GATC 1 cut(s) 489
MbiI CCGCTC 1 cut(s) 452
MboI GATC 1 cut(s) 487
MboII GAAGA 4 cut(s) 272, 376, 551, 556
MlsI TGGCCA 1 cut(s) 701
MluNI TGGCCA 1 cut(s) 701
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 4 cut(s) 37, 67, 612, 641
Mox20I TGGCCA 1 cut(s) 701
MscI TGGCCA 1 cut(s) 701
MseI TTAA 2 cut(s) 35, 723
MslI CAYNNNNRTG 1 cut(s) 119
Msp20I TGGCCA 1 cut(s) 701
MspR9I CCNGG 1 cut(s) 380
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 1 cut(s) 380
MwoI GCNNNNNNNGC 7 cut(s) 23, 130, 332, 458, 503, 753, 762
NdeII GATC 1 cut(s) 487
NheI GCTAGC 1 cut(s) 326
NlaIII CATG 1 cut(s) 616
NlaIV GGNNCC 3 cut(s) 10, 25, 318
NmuCI GTSAC 2 cut(s) 342, 538
NspI RCATGY 1 cut(s) 616
PceI AGGCCT 1 cut(s) 547
PctI GAATGC 1 cut(s) 328
PfeI GAWTC 2 cut(s) 155, 414
PkrI GCNGC 4 cut(s) 135, 463, 499, 589
PshAI GACNNNNGTC 1 cut(s) 380
Psp1406I AACGTT 1 cut(s) 532
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 3 cut(s) 10, 25, 318
RsaI GTAC 2 cut(s) 686, 740
RsaNI GTAC 2 cut(s) 685, 739
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 2 cut(s) 35, 723
SatI GCNGC 4 cut(s) 134, 462, 498, 588
Sau3AI GATC 1 cut(s) 487
ScrFI CCNGG 1 cut(s) 380
SfaNI GCATC 2 cut(s) 97, 176
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 2 cut(s) 58, 222
SmoI CTYRAG 2 cut(s) 58, 222
SseBI AGGCCT 1 cut(s) 547
SsiI CCGC 5 cut(s) 134, 173, 188, 450, 588
SspMI CTAG 3 cut(s) 327, 543, 769
StuI AGGCCT 1 cut(s) 547
StyD4I CCNGG 1 cut(s) 378
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 2 cut(s) 254, 673
TaiI ACGT 1 cut(s) 535
TaqI TCGA 1 cut(s) 650
TaqII GACCGA 1 cut(s) 623
TatI WGTACW 1 cut(s) 738
TauI GCSGC 2 cut(s) 136, 590
TfiI GAWTC 2 cut(s) 155, 414
Tru1I TTAA 2 cut(s) 35, 723
Tru9I TTAA 2 cut(s) 35, 723
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 2 cut(s) 342, 538
TseI GCWGC 2 cut(s) 461, 497
Tsp45I GTSAC 2 cut(s) 342, 538
TspDTI ATGAA 1 cut(s) 543
TspRI CASTG 1 cut(s) 403
XceI RCATGY 1 cut(s) 616
XspI CTAG 3 cut(s) 327, 543, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.