Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0022351

Physical Location & Seq

Genomic Coordinates Forward (+)

4232397 .. 4233356

Gene Structure 960 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1062100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 960 bp

ATGGAAACTGATGAACAGTTATGTTCAGATTGCAAGAAATACACACAAGTTGTGATTGACCACCGGTCCGGTGACACGATCTGCACCGACTGTGGTTTGATTGTTGAAGCCTTCAGCATCGATGTCACCGCCGAGTGGCGAAATTTTGCGGATAAGGATCCCAAGGAAGACCGGGCTCGCGTCGGTGCATCCTTGAATCCTTTGCTGGATAATGGCATTCTTACCACTTGTATTTCCCAAGAAAAGAAGCAAAACAAGAACAAGGATAACGCCGAAGGCGTTGTTCCTCCAAATAACCGGTTGAATAACGTTGAGACGTCGAACCCTCAAATGTCTCTCGCAAAAGCTACAGAACACCTCGAAGCCATGGCTTGCCGGCTAGACAATCATGGCCTTCCCGAAGGCGTACTCTATGATGCCAAAGTGTTATACAAGAAGGCCGTTGACAAGAAGATCTGCAGGGGGAGAAAGTTTGGTGCTATGATGGCGGCTTGCCTGTTCCTTGCTTGCCAAGAAAACCATAGTTCGCGAACACCAAAGGAAATCGCCGGGGCTGCCAATGGACCCTCCATGAAAGAAATTACCAAGATGGTAAAATTGTTGAAAGAAGAGCTGGAGCTCGTTTCCAAAGCTACAGACGCTGGGGACCTATGGCGGCGATACTGCTCGAATCTTGGCATGAACAGTATTCAGGATCGGAAGGCTGTGGAAGAGACTCTGAAGAACTTGGCGAAGCTTGACATAAGGAGAAGCCCTACATCTGTTGTGGCAGCGGTTCTGTACATGATAGTGAAGCTTGCTTCCAATGGCAAGACTGTTGAAGATGTCCAGCAAGAAACTGGTGCTTCCGTGGGGACGATTAAAAGCACATACAAGGAGATTTATCCCTATGCTTCAACGATAGTACCCAACTGGTACTGCAAGTACTTGGAGGACATCAAGAATCTGAACACTCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000769 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006949 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009960 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko03022 ko05169 ko05203 map03022 map05169 map05203

Pfam Domains

Protein Families

TFIIB TF_Zn_Ribbon

Protein Analysis

320

Amino Acids

35.48

Weight (kDa)

7.92

Isoelectric Point (pI)

35.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_Ribbon_TF	PF08271	8 - 49	8.9e-12	TFIIB zinc-binding
TFIIB	PF00382	111 - 186	2.1e-13	Transcription factor TFIIB repeat
TFIIB	PF00382	214 - 295	3.3e-06	Transcription factor TFIIB repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0022351)

Species	Orthologous Gene IDs
malus_domestica	MD09G1062100.v1.1 MD09G1062200.v1.1
pyrus_communis	pycom111g05240 pycom111g05250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	320
AccII	CGCG	2 cut(s)	180, 529
AciI	CCGC	5 cut(s)	129, 149, 488, 655, 773
AclI	AACGTT	1 cut(s)	309
AclWI	GGATC	3 cut(s)	152, 165, 702
AcsI	RAATTY	1 cut(s)	142
AcuI	CTGAAG	2 cut(s)	97, 740
AcyI	GRCGYC	1 cut(s)	317
AfaI	GTAC	5 cut(s)	408, 782, 906, 917, 926
AfiI	CCNNNNNNNGG	1 cut(s)	135
AgeI	ACCGGT	2 cut(s)	63, 297
AgsI	TTSAA	6 cut(s)	107, 196, 304, 604, 821, 897
AhdI	GACNNNNNGTC	1 cut(s)	64
AluBI	AGCT	6 cut(s)	347, 613, 619, 632, 736, 796
AluI	AGCT	6 cut(s)	347, 613, 619, 632, 736, 796
Alw21I	GWGCWC	1 cut(s)	621
Alw26I	GTCTC	3 cut(s)	308, 339, 707
AlwI	GGATC	3 cut(s)	152, 165, 702
AlwNI	CAGNNNCTG	1 cut(s)	641
AoxI	GGCC	2 cut(s)	391, 438
ApeKI	GCWGC	2 cut(s)	554, 770
ApoI	RAATTY	1 cut(s)	142
AsiGI	ACCGGT	2 cut(s)	63, 297
AspS9I	GGNCC	3 cut(s)	66, 563, 646
AsuC2I	CCSGG	2 cut(s)	173, 550
AsuHPI	GGTGA	2 cut(s)	83, 118
AvaII	GGWCC	3 cut(s)	66, 563, 646
BaeI	ACNNNNGTAYC	2 cut(s)	888, 921
BamHI	GGATCC	1 cut(s)	157
BanII	GRGCYC	2 cut(s)	178, 621
BbsI	GAAGAC	1 cut(s)	174
Bbv12I	GWGCWC	1 cut(s)	621
BbvI	GCAGC	2 cut(s)	541, 782
BccI	CCATC	2 cut(s)	478, 583
BceAI	ACGGC	1 cut(s)	425
BcnI	CCSGG	2 cut(s)	173, 550
BcoDI	GTCTC	3 cut(s)	308, 339, 707
BfaI	CTAG	1 cut(s)	380
BfmI	CTRYAG	3 cut(s)	348, 457, 633
BglII	AGATCT	1 cut(s)	453
BisI	GCNGC	4 cut(s)	489, 555, 656, 771
BlsI	GCNGC	4 cut(s)	490, 556, 657, 772
BmcAI	AGTACT	1 cut(s)	926
Bme1390I	CCNGG	2 cut(s)	173, 550
Bme18I	GGWCC	3 cut(s)	66, 563, 646
BmeRI	GACNNNNNGTC	1 cut(s)	64
BmgT120I	GGNCC	3 cut(s)	66, 563, 646
BmiI	GGNNCC	3 cut(s)	159, 565, 647
BmrFI	CCNGG	2 cut(s)	173, 550
BmsI	GCATC	3 cut(s)	126, 197, 406
BpiI	GAAGAC	1 cut(s)	174
BpmI	CTGGAG	1 cut(s)	635
BpuMI	CCSGG	2 cut(s)	173, 550
Bsa29I	ATCGAT	1 cut(s)	120
BsaBI	GATNNNNATC	1 cut(s)	156
BsaHI	GRCGYC	1 cut(s)	317
BsaJI	CCNNGG	4 cut(s)	162, 366, 549, 849
BsaWI	WCCGGW	3 cut(s)	63, 68, 297
Bsc4I	CCNNNNNNNGG	1 cut(s)	135
Bse118I	RCCGGY	3 cut(s)	63, 297, 375
Bse1I	ACTGG	2 cut(s)	844, 917
Bse8I	GATNNNNATC	1 cut(s)	156
BseCI	ATCGAT	1 cut(s)	120
BseDI	CCNNGG	4 cut(s)	162, 366, 549, 849
BseGI	GGATG	1 cut(s)	188
BseJI	GATNNNNATC	1 cut(s)	156
BseLI	CCNNNNNNNGG	1 cut(s)	135
BseNI	ACTGG	2 cut(s)	844, 917
BseXI	GCAGC	2 cut(s)	541, 782
BseYI	CCCAGC	1 cut(s)	641
BsgI	GTGCAG	1 cut(s)	67
Bsh1236I	CGCG	2 cut(s)	180, 529
BshFI	GGCC	2 cut(s)	393, 440
BshTI	ACCGGT	2 cut(s)	63, 297
BshVI	ATCGAT	1 cut(s)	120
BsiHKAI	GWGCWC	1 cut(s)	621
BsiSI	CCGG	6 cut(s)	64, 69, 172, 298, 376, 549
BslFI	GGGAC	2 cut(s)	659, 868
BslI	CCNNNNNNNGG	1 cut(s)	135
BsmAI	GTCTC	3 cut(s)	308, 339, 707
BsmBI	CGTCTC	1 cut(s)	308
BsmFI	GGGAC	2 cut(s)	659, 868
BsmI	GAATGC	1 cut(s)	216
BsnI	GGCC	2 cut(s)	393, 440
Bsp1286I	GDGCHC	2 cut(s)	178, 621
Bsp1407I	TGTACA	1 cut(s)	780
Bsp143I	GATC	4 cut(s)	78, 157, 453, 694
Bsp19I	CCATGG	1 cut(s)	366
Bsp68I	TCGCGA	1 cut(s)	529
BspACI	CCGC	5 cut(s)	129, 149, 488, 655, 773
BspANI	GGCC	2 cut(s)	393, 440
BspDI	ATCGAT	1 cut(s)	120
BspFNI	CGCG	2 cut(s)	180, 529
BspLI	GGNNCC	3 cut(s)	159, 565, 647
BspMAI	CTGCAG	1 cut(s)	461
BspPI	GGATC	3 cut(s)	152, 165, 702
BspQI	GCTCTTC	1 cut(s)	603
BsrFI	RCCGGY	3 cut(s)	63, 297, 375
BsrGI	TGTACA	1 cut(s)	780
BsrI	ACTGG	2 cut(s)	844, 917
BssAI	RCCGGY	3 cut(s)	63, 297, 375
BssECI	CCNNGG	4 cut(s)	162, 366, 549, 849
BssMI	GATC	4 cut(s)	78, 157, 453, 694
BssNI	GRCGYC	1 cut(s)	317
BssT1I	CCWWGG	2 cut(s)	162, 366
Bst4CI	ACNGT	4 cut(s)	18, 92, 686, 817
Bst6I	CTCTTC	2 cut(s)	603, 705
BstACI	GRCGYC	1 cut(s)	317
BstAUI	TGTACA	1 cut(s)	780
BstC8I	GCNNGC	6 cut(s)	178, 373, 377, 493, 508, 798
BstDSI	CCRYGG	2 cut(s)	366, 849
BstF5I	GGATG	1 cut(s)	188
BstFNI	CGCG	2 cut(s)	180, 529
BstKTI	GATC	4 cut(s)	81, 160, 456, 697
BstMAI	GTCTC	3 cut(s)	308, 339, 707
BstMBI	GATC	4 cut(s)	78, 157, 453, 694
BstMWI	GCNNNNNNNGC	3 cut(s)	485, 554, 638
BstSCI	CCNGG	2 cut(s)	171, 548
BstSFI	CTRYAG	3 cut(s)	348, 457, 633
BstUI	CGCG	2 cut(s)	180, 529
BstV1I	GCAGC	2 cut(s)	541, 782
BstV2I	GAAGAC	1 cut(s)	174
BstX2I	RGATCY	2 cut(s)	157, 453
BstYI	RGATCY	2 cut(s)	157, 453
Bsu15I	ATCGAT	1 cut(s)	120
BsuRI	GGCC	2 cut(s)	393, 440
BsuTUI	ATCGAT	1 cut(s)	120
BtgI	CCRYGG	2 cut(s)	366, 849
BtsCI	GGATG	1 cut(s)	188
BtuMI	TCGCGA	1 cut(s)	529
Cac8I	GCNNGC	6 cut(s)	178, 373, 377, 493, 508, 798
CaiI	CAGNNNCTG	1 cut(s)	641
Cfr10I	RCCGGY	3 cut(s)	63, 297, 375
Cfr13I	GGNCC	3 cut(s)	66, 563, 646
ClaI	ATCGAT	1 cut(s)	120
CpoI	CGGWCCG	1 cut(s)	66
CseI	GACGC	2 cut(s)	169, 647
Csp6I	GTAC	5 cut(s)	407, 781, 905, 916, 925
CspAI	ACCGGT	2 cut(s)	63, 297
CspI	CGGWCCG	1 cut(s)	66
CviAII	CATG	5 cut(s)	367, 389, 571, 679, 784
CviQI	GTAC	5 cut(s)	407, 781, 905, 916, 925
DpnI	GATC	4 cut(s)	80, 159, 455, 696
DpnII	GATC	4 cut(s)	78, 157, 453, 694
DriI	GACNNNNNGTC	1 cut(s)	64
Eam1104I	CTCTTC	2 cut(s)	603, 705
Eam1105I	GACNNNNNGTC	1 cut(s)	64
EarI	CTCTTC	2 cut(s)	603, 705
Ecl136II	GAGCTC	1 cut(s)	619
Eco130I	CCWWGG	2 cut(s)	162, 366
Eco24I	GRGCYC	2 cut(s)	178, 621
Eco47I	GGWCC	3 cut(s)	66, 563, 646
Eco53kI	GAGCTC	1 cut(s)	619
Eco57I	CTGAAG	2 cut(s)	97, 740
EcoICRI	GAGCTC	1 cut(s)	619
EcoO109I	RGGNCCY	1 cut(s)	646
EcoT14I	CCWWGG	2 cut(s)	162, 366
EcoT38I	GRGCYC	2 cut(s)	178, 621
ErhI	CCWWGG	2 cut(s)	162, 366
Esp3I	CGTCTC	1 cut(s)	308
FaeI	CATG	5 cut(s)	370, 392, 574, 682, 787
FaqI	GGGAC	2 cut(s)	659, 868
FatI	CATG	5 cut(s)	366, 388, 570, 678, 783
Fnu4HI	GCNGC	4 cut(s)	489, 555, 656, 771
FokI	GGATG	1 cut(s)	175
FriOI	GRGCYC	2 cut(s)	178, 621
Fsp4HI	GCNGC	4 cut(s)	489, 555, 656, 771
FspBI	CTAG	1 cut(s)	380
GluI	GCNGC	4 cut(s)	489, 555, 656, 771
GsaI	CCCAGC	1 cut(s)	645
GsuI	CTGGAG	1 cut(s)	635
HaeIII	GGCC	2 cut(s)	393, 440
HapII	CCGG	6 cut(s)	64, 69, 172, 298, 376, 549
HgaI	GACGC	2 cut(s)	169, 647
Hin1I	GRCGYC	1 cut(s)	317
Hin1II	CATG	5 cut(s)	370, 392, 574, 682, 787
HincII	GTYRAC	1 cut(s)	445
HindII	GTYRAC	1 cut(s)	445
HindIII	AAGCTT	2 cut(s)	734, 794
HinfI	GANTC	4 cut(s)	196, 670, 715, 943
HpaII	CCGG	6 cut(s)	64, 69, 172, 298, 376, 549
HphI	GGTGA	2 cut(s)	83, 118
Hpy166II	GTNNAC	1 cut(s)	445
Hpy188I	TCNGA	4 cut(s)	28, 699, 720, 948
Hpy188III	TCNNGA	4 cut(s)	398, 528, 692, 940
Hpy8I	GTNNAC	1 cut(s)	445
Hpy99I	CGWCG	2 cut(s)	185, 322
HpyAV	CCTTC	6 cut(s)	121, 269, 395, 404, 430, 694
HpyCH4III	ACNGT	4 cut(s)	18, 92, 686, 817
HpyCH4IV	ACGT	2 cut(s)	309, 317
HpyCH4V	TGCA	5 cut(s)	33, 84, 188, 459, 921
HpyF10VI	GCNNNNNNNGC	3 cut(s)	485, 554, 638
HpySE526I	ACGT	2 cut(s)	309, 317
Hsp92I	GRCGYC	1 cut(s)	317
Hsp92II	CATG	5 cut(s)	370, 392, 574, 682, 787
KroI	GCCGGC	1 cut(s)	375
KroNI	GCCGGC	1 cut(s)	377
Kzo9I	GATC	4 cut(s)	78, 157, 453, 694
LguI	GCTCTTC	1 cut(s)	603
LmnI	GCTCC	1 cut(s)	616
Lsp1109I	GCAGC	2 cut(s)	541, 782
LweI	GCATC	3 cut(s)	126, 197, 406
MaeI	CTAG	1 cut(s)	380
MaeII	ACGT	2 cut(s)	309, 317
MaeIII	GTNAC	2 cut(s)	71, 124
MalI	GATC	4 cut(s)	80, 159, 455, 696
MboI	GATC	4 cut(s)	78, 157, 453, 694
MboII	GAAGA	6 cut(s)	179, 463, 620, 722, 733, 833
MflI	RGATCY	2 cut(s)	157, 453
MhlI	GDGCHC	2 cut(s)	178, 621
MluCI	AATT	3 cut(s)	142, 579, 596
MlyI	GAGTC	1 cut(s)	709
MnlI	CCTC	5 cut(s)	297, 336, 368, 577, 925
MroNI	GCCGGC	1 cut(s)	375
MseI	TTAA	1 cut(s)	861
MslI	CAYNNNNRTG	1 cut(s)	788
MspA1I	CMGCKG	1 cut(s)	773
MspI	CCGG	6 cut(s)	64, 69, 172, 298, 376, 549
MspR9I	CCNGG	2 cut(s)	173, 550
Mva1269I	GAATGC	1 cut(s)	216
MvnI	CGCG	2 cut(s)	180, 529
MwoI	GCNNNNNNNGC	3 cut(s)	485, 554, 638
NaeI	GCCGGC	1 cut(s)	377
NciI	CCSGG	2 cut(s)	173, 550
NcoI	CCATGG	1 cut(s)	366
NdeII	GATC	4 cut(s)	78, 157, 453, 694
NgoMIV	GCCGGC	1 cut(s)	375
NlaIII	CATG	5 cut(s)	370, 392, 574, 682, 787
NlaIV	GGNNCC	3 cut(s)	159, 565, 647
NmeAIII	GCCGAG	1 cut(s)	157
NmuCI	GTSAC	2 cut(s)	71, 124
NruI	TCGCGA	1 cut(s)	529
PciSI	GCTCTTC	1 cut(s)	603
PcsI	WCGNNNNNNNCGW	1 cut(s)	276
PctI	GAATGC	1 cut(s)	216
PdiI	GCCGGC	1 cut(s)	377
PfeI	GAWTC	3 cut(s)	196, 670, 943
PinAI	ACCGGT	2 cut(s)	63, 297
PkrI	GCNGC	4 cut(s)	490, 556, 657, 772
PleI	GAGTC	1 cut(s)	709
PpsI	GAGTC	1 cut(s)	709
PpuMI	RGGWCCY	1 cut(s)	646
Psp124BI	GAGCTC	1 cut(s)	621
Psp1406I	AACGTT	1 cut(s)	309
Psp5II	RGGWCCY	1 cut(s)	646
PspFI	CCCAGC	1 cut(s)	641
PspN4I	GGNNCC	3 cut(s)	159, 565, 647
PspPI	GGNCC	3 cut(s)	66, 563, 646
PspPPI	RGGWCCY	1 cut(s)	646
PstI	CTGCAG	1 cut(s)	461
PstNI	CAGNNNCTG	1 cut(s)	641
PsuI	RGATCY	2 cut(s)	157, 453
RruI	TCGCGA	1 cut(s)	529
RsaI	GTAC	5 cut(s)	408, 782, 906, 917, 926
RsaNI	GTAC	5 cut(s)	407, 781, 905, 916, 925
RseI	CAYNNNNRTG	1 cut(s)	788
Rsr2I	CGGWCCG	1 cut(s)	66
RsrII	CGGWCCG	1 cut(s)	66
SacI	GAGCTC	1 cut(s)	621
SapI	GCTCTTC	1 cut(s)	603
SaqAI	TTAA	1 cut(s)	861
SatI	GCNGC	4 cut(s)	489, 555, 656, 771
Sau3AI	GATC	4 cut(s)	78, 157, 453, 694
Sau96I	GGNCC	3 cut(s)	66, 563, 646
ScaI	AGTACT	1 cut(s)	926
SchI	GAGTC	1 cut(s)	709
ScrFI	CCNGG	2 cut(s)	173, 550
SduI	GDGCHC	2 cut(s)	178, 621
SfaNI	GCATC	3 cut(s)	126, 197, 406
SfcI	CTRYAG	3 cut(s)	348, 457, 633
SinI	GGWCC	3 cut(s)	66, 563, 646
SmiMI	CAYNNNNRTG	1 cut(s)	788
Sse9I	AATT	3 cut(s)	142, 579, 596
SsiI	CCGC	5 cut(s)	129, 149, 488, 655, 773
SspMI	CTAG	1 cut(s)	380
SstI	GAGCTC	1 cut(s)	621
StyD4I	CCNGG	2 cut(s)	171, 548
StyI	CCWWGG	2 cut(s)	162, 366
TaaI	ACNGT	4 cut(s)	18, 92, 686, 817
TaiI	ACGT	2 cut(s)	312, 320
TaqI	TCGA	4 cut(s)	120, 320, 360, 668
TasI	AATT	3 cut(s)	142, 579, 596
TatI	WGTACW	2 cut(s)	780, 924
TauI	GCSGC	2 cut(s)	491, 658
TfiI	GAWTC	3 cut(s)	196, 670, 943
Tru1I	TTAA	1 cut(s)	861
Tru9I	TTAA	1 cut(s)	861
TseFI	GTSAC	2 cut(s)	71, 124
TseI	GCWGC	2 cut(s)	554, 770
Tsp45I	GTSAC	2 cut(s)	71, 124
TspDTI	ATGAA	3 cut(s)	27, 587, 695
TspGWI	ACGGA	1 cut(s)	838
VpaK11BI	GGWCC	3 cut(s)	66, 563, 646
XapI	RAATTY	1 cut(s)	142
XcmI	CCANNNNNNNNNTGG	1 cut(s)	836
XspI	CTAG	1 cut(s)	380
ZraI	GACGTC	1 cut(s)	318
ZrmI	AGTACT	1 cut(s)	926

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.