Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0022351

Physical Location & Seq

Genomic Coordinates Forward (+)

4234408 .. 4235366

Gene Structure 959 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1062200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 903 bp

ATGGAAACCGATGAACAGTTCCGTTCATATTGCAAGAAATACACACAAGTTGTGATCGACCACCAGTCGGGTGACACAATCTGCACTGACTGTGGTTTGATTGTTGGAGCCTACTGCATCGATGTCACCGCCGAGTGGCGAAAGTTTGCGGATAAGGATCCCAAGGAGGACCGGACTCGTGTCGGTACTTCCTTGGATCCTTTGCTGGATAATGGCCTTCTTACCACTTGTATTTCCAAAGAAAAGAAGCAAAACAAGAACAAGGACAACACCGATGGTGTTGTTCCTCCATATAACCGGTTGAATAACGTTGCGACGTCGAACCCCCAAACGTCTCTCACAAAAGCTACAGAATACCTCGAAGCCATGGCTCACCACCTAGACAAGCATGGCCTTCCCGAAGGCGGGGAGAAAGTTTGGTGCTATGATGGCCGCTTGCCTGTTCCTTGCCTGCCAAGAAAACCATATTCGCGAACACCAAAGGAAATCGCCGGGGCTGCCAACGGACCCTCAATGAAAGAAATTACCAAGATGGTAAAAGTGTTGAAAGAAGAGCTAGAGCTCGGTTCCAAAGCTACAGACGCCGGGGACCTATGGCGGAGATACTGCTCAAATCTTGGCATGAACAGTATTCAGGATCGGAGGGCTGTGGAAGAAACTTTGAAGAACTTGGCGAAGCTTGACATAAGGAGAAGCCCTACATCTGTTGTGGCAGCGGTTTTGTACATGATAGTGAAGCTTGCTTCCAATGGAAAAACTGTTGAAGATGTCCAGCAAGAAACTGGTGCTGCCGTGGGGACGATTAAAAGCACATACAAGGAGATTTATCTCTATTCTTCGACAATAATACCCAATTGGTATTGCAAGTACTTGGAGGACCTCAAGAAATTGAACAGTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000769 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006949 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009960 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko03022 ko05169 ko05203 map03022 map05169 map05203

Pfam Domains

Protein Families

TFIIB TF_Zn_Ribbon

Protein Analysis

301

Amino Acids

33.62

Weight (kDa)

8.24

Isoelectric Point (pI)

33.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_Ribbon_TF	PF08271	10 - 49	1.7e-08	TFIIB zinc-binding
TFIIB	PF00382	195 - 276	6.1e-06	Transcription factor TFIIB repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0022351)

Species	Orthologous Gene IDs
malus_domestica	MD09G1062100.v1.1 MD09G1062200.v1.1
pyrus_communis	pycom111g05240 pycom111g05250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	320
AccII	CGCG	1 cut(s)	472
AciI	CCGC	6 cut(s)	129, 149, 405, 433, 598, 716
AclI	AACGTT	1 cut(s)	309
AclWI	GGATC	5 cut(s)	152, 165, 191, 204, 645
AcoI	YGGCCR	1 cut(s)	430
AcyI	GRCGYC	2 cut(s)	317, 582
AfaI	GTAC	3 cut(s)	187, 725, 869
AfiI	CCNNNNNNNGG	4 cut(s)	67, 135, 404, 405
AgeI	ACCGGT	1 cut(s)	297
AgsI	TTSAA	5 cut(s)	304, 547, 664, 764, 892
AhdI	GACNNNNNGTC	1 cut(s)	64
AluBI	AGCT	6 cut(s)	347, 556, 562, 575, 679, 739
AluI	AGCT	6 cut(s)	347, 556, 562, 575, 679, 739
Alw21I	GWGCWC	1 cut(s)	564
Alw26I	GTCTC	1 cut(s)	339
AlwI	GGATC	5 cut(s)	152, 165, 191, 204, 645
AoxI	GGCC	3 cut(s)	214, 391, 430
ApeKI	GCWGC	3 cut(s)	497, 713, 788
AsiGI	ACCGGT	1 cut(s)	297
AspS9I	GGNCC	4 cut(s)	169, 506, 589, 877
AsuC2I	CCSGG	2 cut(s)	493, 586
AsuHPI	GGTGA	3 cut(s)	83, 118, 365
AvaII	GGWCC	4 cut(s)	169, 506, 589, 877
BamHI	GGATCC	2 cut(s)	157, 196
BanII	GRGCYC	1 cut(s)	564
BauI	CACGAG	1 cut(s)	177
Bbv12I	GWGCWC	1 cut(s)	564
BbvI	GCAGC	3 cut(s)	484, 725, 775
BccI	CCATC	3 cut(s)	269, 422, 526
BceAI	ACGGC	1 cut(s)	776
BcnI	CCSGG	2 cut(s)	493, 586
BcoDI	GTCTC	1 cut(s)	339
BfaI	CTAG	2 cut(s)	380, 557
BfmI	CTRYAG	2 cut(s)	348, 576
BisI	GCNGC	4 cut(s)	433, 498, 714, 789
BlsI	GCNGC	4 cut(s)	434, 499, 715, 790
BmcAI	AGTACT	1 cut(s)	869
Bme1390I	CCNGG	2 cut(s)	493, 586
Bme18I	GGWCC	4 cut(s)	169, 506, 589, 877
BmeRI	GACNNNNNGTC	1 cut(s)	64
BmgT120I	GGNCC	4 cut(s)	169, 506, 589, 877
BmiI	GGNNCC	6 cut(s)	109, 159, 198, 508, 568, 590
BmrFI	CCNGG	2 cut(s)	493, 586
BmsI	GCATC	1 cut(s)	126
BoxI	GACNNNNGTC	1 cut(s)	179
BpuEI	CTTGAG	1 cut(s)	866
BpuMI	CCSGG	2 cut(s)	493, 586
Bsa29I	ATCGAT	1 cut(s)	120
BsaBI	GATNNNNATC	1 cut(s)	156
BsaHI	GRCGYC	2 cut(s)	317, 582
BsaJI	CCNNGG	6 cut(s)	162, 192, 366, 492, 585, 792
BsaWI	WCCGGW	2 cut(s)	171, 297
Bsc4I	CCNNNNNNNGG	4 cut(s)	67, 135, 404, 405
Bse118I	RCCGGY	1 cut(s)	297
Bse1I	ACTGG	2 cut(s)	64, 787
Bse8I	GATNNNNATC	1 cut(s)	156
BseCI	ATCGAT	1 cut(s)	120
BseDI	CCNNGG	6 cut(s)	162, 192, 366, 492, 585, 792
BseJI	GATNNNNATC	1 cut(s)	156
BseLI	CCNNNNNNNGG	4 cut(s)	67, 135, 404, 405
BseNI	ACTGG	2 cut(s)	64, 787
BseXI	GCAGC	3 cut(s)	484, 725, 775
BsgI	GTGCAG	1 cut(s)	67
Bsh1236I	CGCG	1 cut(s)	472
BshFI	GGCC	3 cut(s)	216, 393, 432
BshTI	ACCGGT	1 cut(s)	297
BshVI	ATCGAT	1 cut(s)	120
BsiHKAI	GWGCWC	1 cut(s)	564
BsiSI	CCGG	4 cut(s)	172, 298, 492, 585
BslFI	GGGAC	2 cut(s)	602, 811
BslI	CCNNNNNNNGG	4 cut(s)	67, 135, 404, 405
BsmAI	GTCTC	1 cut(s)	339
BsmBI	CGTCTC	1 cut(s)	339
BsmFI	GGGAC	2 cut(s)	602, 811
BsnI	GGCC	3 cut(s)	216, 393, 432
Bsp1286I	GDGCHC	1 cut(s)	564
Bsp1407I	TGTACA	1 cut(s)	723
Bsp143I	GATC	4 cut(s)	54, 157, 196, 637
Bsp19I	CCATGG	1 cut(s)	366
Bsp68I	TCGCGA	1 cut(s)	472
BspACI	CCGC	6 cut(s)	129, 149, 405, 433, 598, 716
BspANI	GGCC	3 cut(s)	216, 393, 432
BspDI	ATCGAT	1 cut(s)	120
BspFNI	CGCG	1 cut(s)	472
BspLI	GGNNCC	6 cut(s)	109, 159, 198, 508, 568, 590
BspPI	GGATC	5 cut(s)	152, 165, 191, 204, 645
BspQI	GCTCTTC	1 cut(s)	546
BsrFI	RCCGGY	1 cut(s)	297
BsrGI	TGTACA	1 cut(s)	723
BsrI	ACTGG	2 cut(s)	64, 787
BssAI	RCCGGY	1 cut(s)	297
BssECI	CCNNGG	6 cut(s)	162, 192, 366, 492, 585, 792
BssMI	GATC	4 cut(s)	54, 157, 196, 637
BssNI	GRCGYC	2 cut(s)	317, 582
BssSI	CACGAG	1 cut(s)	177
BssT1I	CCWWGG	3 cut(s)	162, 192, 366
Bst2BI	CACGAG	1 cut(s)	177
Bst4CI	ACNGT	5 cut(s)	18, 92, 629, 760, 896
Bst6I	CTCTTC	1 cut(s)	546
BstACI	GRCGYC	2 cut(s)	317, 582
BstAUI	TGTACA	1 cut(s)	723
BstC8I	GCNNGC	3 cut(s)	437, 452, 741
BstDSI	CCRYGG	2 cut(s)	366, 792
BstFNI	CGCG	1 cut(s)	472
BstKTI	GATC	4 cut(s)	57, 160, 199, 640
BstMAI	GTCTC	1 cut(s)	339
BstMBI	GATC	4 cut(s)	54, 157, 196, 637
BstMWI	GCNNNNNNNGC	3 cut(s)	429, 497, 581
BstPAI	GACNNNNGTC	1 cut(s)	179
BstSCI	CCNGG	2 cut(s)	491, 584
BstSFI	CTRYAG	2 cut(s)	348, 576
BstUI	CGCG	1 cut(s)	472
BstV1I	GCAGC	3 cut(s)	484, 725, 775
BstX2I	RGATCY	2 cut(s)	157, 196
BstYI	RGATCY	2 cut(s)	157, 196
Bsu15I	ATCGAT	1 cut(s)	120
BsuRI	GGCC	3 cut(s)	216, 393, 432
BsuTUI	ATCGAT	1 cut(s)	120
BtgI	CCRYGG	2 cut(s)	366, 792
BtsIMutI	CAGTG	1 cut(s)	84
BtuMI	TCGCGA	1 cut(s)	472
Cac8I	GCNNGC	3 cut(s)	437, 452, 741
Cfr10I	RCCGGY	1 cut(s)	297
Cfr13I	GGNCC	4 cut(s)	169, 506, 589, 877
ClaI	ATCGAT	1 cut(s)	120
CseI	GACGC	1 cut(s)	590
Csp6I	GTAC	3 cut(s)	186, 724, 868
CspAI	ACCGGT	1 cut(s)	297
CviAII	CATG	4 cut(s)	367, 389, 622, 727
CviQI	GTAC	3 cut(s)	186, 724, 868
DpnI	GATC	4 cut(s)	56, 159, 198, 639
DpnII	GATC	4 cut(s)	54, 157, 196, 637
DriI	GACNNNNNGTC	1 cut(s)	64
EaeI	YGGCCR	1 cut(s)	430
Eam1104I	CTCTTC	1 cut(s)	546
Eam1105I	GACNNNNNGTC	1 cut(s)	64
EarI	CTCTTC	1 cut(s)	546
EciI	GGCGGA	1 cut(s)	613
Ecl136II	GAGCTC	1 cut(s)	562
Eco130I	CCWWGG	3 cut(s)	162, 192, 366
Eco24I	GRGCYC	1 cut(s)	564
Eco47I	GGWCC	4 cut(s)	169, 506, 589, 877
Eco53kI	GAGCTC	1 cut(s)	562
EcoICRI	GAGCTC	1 cut(s)	562
EcoO109I	RGGNCCY	2 cut(s)	589, 877
EcoT14I	CCWWGG	3 cut(s)	162, 192, 366
EcoT38I	GRGCYC	1 cut(s)	564
ErhI	CCWWGG	3 cut(s)	162, 192, 366
Esp3I	CGTCTC	1 cut(s)	339
FaeI	CATG	4 cut(s)	370, 392, 625, 730
FaqI	GGGAC	2 cut(s)	602, 811
FatI	CATG	4 cut(s)	366, 388, 621, 726
FauI	CCCGC	1 cut(s)	398
Fnu4HI	GCNGC	4 cut(s)	433, 498, 714, 789
FriOI	GRGCYC	1 cut(s)	564
Fsp4HI	GCNGC	4 cut(s)	433, 498, 714, 789
FspBI	CTAG	2 cut(s)	380, 557
GluI	GCNGC	4 cut(s)	433, 498, 714, 789
HaeIII	GGCC	3 cut(s)	216, 393, 432
HapII	CCGG	4 cut(s)	172, 298, 492, 585
HgaI	GACGC	1 cut(s)	590
Hin1I	GRCGYC	2 cut(s)	317, 582
Hin1II	CATG	4 cut(s)	370, 392, 625, 730
HindIII	AAGCTT	2 cut(s)	677, 737
HinfI	GANTC	1 cut(s)	175
HpaII	CCGG	4 cut(s)	172, 298, 492, 585
HphI	GGTGA	3 cut(s)	83, 118, 365
Hpy188I	TCNGA	1 cut(s)	642
Hpy188III	TCNNGA	4 cut(s)	398, 471, 635, 883
Hpy99I	CGWCG	2 cut(s)	319, 322
HpyAV	CCTTC	3 cut(s)	227, 395, 404
HpyCH4III	ACNGT	5 cut(s)	18, 92, 629, 760, 896
HpyCH4IV	ACGT	3 cut(s)	309, 317, 332
HpyCH4V	TGCA	4 cut(s)	33, 84, 117, 864
HpyF10VI	GCNNNNNNNGC	3 cut(s)	429, 497, 581
HpySE526I	ACGT	3 cut(s)	309, 317, 332
Hsp92I	GRCGYC	2 cut(s)	317, 582
Hsp92II	CATG	4 cut(s)	370, 392, 625, 730
Kzo9I	GATC	4 cut(s)	54, 157, 196, 637
LguI	GCTCTTC	1 cut(s)	546
LmnI	GCTCC	1 cut(s)	107
Lsp1109I	GCAGC	3 cut(s)	484, 725, 775
LweI	GCATC	1 cut(s)	126
MaeI	CTAG	2 cut(s)	380, 557
MaeII	ACGT	3 cut(s)	309, 317, 332
MaeIII	GTNAC	2 cut(s)	71, 124
MalI	GATC	4 cut(s)	56, 159, 198, 639
MboI	GATC	4 cut(s)	54, 157, 196, 637
MboII	GAAGA	5 cut(s)	563, 665, 676, 776, 828
MfeI	CAATTG	1 cut(s)	853
MflI	RGATCY	2 cut(s)	157, 196
MhlI	GDGCHC	1 cut(s)	564
MluCI	AATT	3 cut(s)	522, 853, 887
MlyI	GAGTC	1 cut(s)	169
MmeI	TCCRAC	1 cut(s)	85
MnlI	CCTC	7 cut(s)	160, 297, 368, 520, 636, 868, 890
MseI	TTAA	1 cut(s)	804
MslI	CAYNNNNRTG	1 cut(s)	731
MspA1I	CMGCKG	1 cut(s)	716
MspI	CCGG	4 cut(s)	172, 298, 492, 585
MspR9I	CCNGG	2 cut(s)	493, 586
MunI	CAATTG	1 cut(s)	853
MvnI	CGCG	1 cut(s)	472
MwoI	GCNNNNNNNGC	3 cut(s)	429, 497, 581
NciI	CCSGG	2 cut(s)	493, 586
NcoI	CCATGG	1 cut(s)	366
NdeII	GATC	4 cut(s)	54, 157, 196, 637
NlaIII	CATG	4 cut(s)	370, 392, 625, 730
NlaIV	GGNNCC	6 cut(s)	109, 159, 198, 508, 568, 590
NmeAIII	GCCGAG	1 cut(s)	157
NmuCI	GTSAC	2 cut(s)	71, 124
NruI	TCGCGA	1 cut(s)	472
PciSI	GCTCTTC	1 cut(s)	546
PinAI	ACCGGT	1 cut(s)	297
PkrI	GCNGC	4 cut(s)	434, 499, 715, 790
PleI	GAGTC	1 cut(s)	169
PpsI	GAGTC	1 cut(s)	169
PpuMI	RGGWCCY	2 cut(s)	589, 877
PshAI	GACNNNNGTC	1 cut(s)	179
Psp124BI	GAGCTC	1 cut(s)	564
Psp1406I	AACGTT	1 cut(s)	309
Psp5II	RGGWCCY	2 cut(s)	589, 877
PspN4I	GGNNCC	6 cut(s)	109, 159, 198, 508, 568, 590
PspPI	GGNCC	4 cut(s)	169, 506, 589, 877
PspPPI	RGGWCCY	2 cut(s)	589, 877
PsuI	RGATCY	2 cut(s)	157, 196
RruI	TCGCGA	1 cut(s)	472
RsaI	GTAC	3 cut(s)	187, 725, 869
RsaNI	GTAC	3 cut(s)	186, 724, 868
RseI	CAYNNNNRTG	1 cut(s)	731
SacI	GAGCTC	1 cut(s)	564
SapI	GCTCTTC	1 cut(s)	546
SaqAI	TTAA	1 cut(s)	804
SatI	GCNGC	4 cut(s)	433, 498, 714, 789
Sau3AI	GATC	4 cut(s)	54, 157, 196, 637
Sau96I	GGNCC	4 cut(s)	169, 506, 589, 877
ScaI	AGTACT	1 cut(s)	869
SchI	GAGTC	1 cut(s)	169
ScrFI	CCNGG	2 cut(s)	493, 586
SduI	GDGCHC	1 cut(s)	564
SfaNI	GCATC	1 cut(s)	126
SfcI	CTRYAG	2 cut(s)	348, 576
SinI	GGWCC	4 cut(s)	169, 506, 589, 877
SmiMI	CAYNNNNRTG	1 cut(s)	731
SmlI	CTYRAG	1 cut(s)	881
SmoI	CTYRAG	1 cut(s)	881
Sse9I	AATT	3 cut(s)	522, 853, 887
SsiI	CCGC	6 cut(s)	129, 149, 405, 433, 598, 716
SspMI	CTAG	2 cut(s)	380, 557
SstI	GAGCTC	1 cut(s)	564
StyD4I	CCNGG	2 cut(s)	491, 584
StyI	CCWWGG	3 cut(s)	162, 192, 366
TaaI	ACNGT	5 cut(s)	18, 92, 629, 760, 896
TaiI	ACGT	3 cut(s)	312, 320, 335
TaqI	TCGA	5 cut(s)	57, 120, 320, 360, 839
TasI	AATT	3 cut(s)	522, 853, 887
TatI	WGTACW	2 cut(s)	723, 867
TauI	GCSGC	1 cut(s)	435
Tru1I	TTAA	1 cut(s)	804
Tru9I	TTAA	1 cut(s)	804
TscAI	CASTG	1 cut(s)	91
TseFI	GTSAC	2 cut(s)	71, 124
TseI	GCWGC	3 cut(s)	497, 713, 788
Tsp45I	GTSAC	2 cut(s)	71, 124
TspDTI	ATGAA	4 cut(s)	15, 27, 530, 638
TspGWI	ACGGA	2 cut(s)	11, 519
TspRI	CASTG	1 cut(s)	91
VpaK11BI	GGWCC	4 cut(s)	169, 506, 589, 877
XcmI	CCANNNNNNNNNTGG	1 cut(s)	779
XspI	CTAG	2 cut(s)	380, 557
ZraI	GACGTC	1 cut(s)	318
ZrmI	AGTACT	1 cut(s)	869

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.