Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0024299

Physical Location & Seq

Genomic Coordinates Reverse (-)

4462537 .. 4464279

Gene Structure 1743 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1065500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 957 bp

ATGGAAGCTAAAGAGTTCTGTAGGGACTGCAAGCAAGAGACAGACGTCGTGTCCGACCACAGGTCGGGTGACACAATCTGCACCTTCTGTGGTTTGATCCTCGAAGCCCGCTACATCGATGAAAGAGCCGAGTGGCGAAATTTTGCCGATCAGAACCCTGAAGAAGACCGGACTCGCGTCGGAAAAGCCTCCGATCCGTTGCTGGATAATGGTGTTCTTTCCACTTGTATATTCAAAGAAAAGAAGAAAAACAACAAGAAGGGTACTGATGCCGATGGTAATTTTCCTCCTAATAAATGGACCAACAGGGCTGTAAACCCTAGCAATTCTCTCCTAAAATCTTTCAAGTACCTCGAAGAGATGGCCGACCGGCTAGACATATACGAAGGCGTTCCGAAATGCATACTGGATCATGCCAAAGGTTTATACAAGAAGGCCGATGATAAGAACTTCTGTAAGGGGAGAAATTCTAACCCCATAATGGCTGCTTGCCTCTTGCTTGCGTTTGAAGAAAGCCCAAATACGCGAACACTAAAGGAAATCACCATGGTTGCCAATGGGCCATCTAAAAAAGAAATCAGCAAGATGAAAGAAGAGTTGAAGAGAAGCCTAGGGATTGCTTCCAAGATCAAAAGCGCCATGGACCTTTGGCCGCGACATTGTTCGAATCTTGGTATGAGTAGTAAAGATAAGAAGGCTGTGGAAGAAGCCCTGAAGAATTTGGCAAATTTTGATATTAGGAGAAACCCTAATTCTGTTGTGGCAGCGGTTATGTACATGGTAGTTCAGCTTTCTAATGGGAATAACTGTTCTGTTCAAGATGTCTCAAAGGCAGCTGATGGTGTTGCTGTAGGGACAACTAAAAGGACATACAAGGATATTTATCCCTATGCTTCACAGATAATACCAACCTGGTTTTGCAGGTTGGAGGACCTCAAGAAACTTATCACTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000769 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006949 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009960 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko03022 ko05169 ko05203 map03022 map05169 map05203

Pfam Domains

Protein Families

TFIIB TF_Zn_Ribbon

Protein Analysis

319

Amino Acids

35.78

Weight (kDa)

8.82

Isoelectric Point (pI)

32.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_Ribbon_TF	PF08271	6 - 48	2.9e-12	TFIIB zinc-binding
TFIIB	PF00382	109 - 186	4.2e-12	Transcription factor TFIIB repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0024299)

Species	Orthologous Gene IDs
malus_domestica	MD09G1065500.v1.1 MD09G1065600.v1.1
pyrus_communis	pycom111g05530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	48
Acc36I	ACCTGC	1 cut(s)	912
AccII	CGCG	3 cut(s)	177, 526, 655
AciI	CCGC	3 cut(s)	109, 653, 767
AclWI	GGATC	3 cut(s)	91, 188, 417
AcoI	YGGCCR	2 cut(s)	363, 650
AcsI	RAATTY	4 cut(s)	139, 466, 718, 727
AcuI	CTGAAG	2 cut(s)	180, 734
AcyI	GRCGYC	1 cut(s)	45
AfaI	GTAC	3 cut(s)	265, 350, 776
AfiI	CCNNNNNNNGG	3 cut(s)	60, 64, 481
AgsI	TTSAA	5 cut(s)	235, 346, 509, 601, 818
AhdI	GACNNNNNGTC	2 cut(s)	49, 61
AjnI	CCWGG	1 cut(s)	911
AloI	GAACNNNNNNTCC	2 cut(s)	793, 825
AluBI	AGCT	3 cut(s)	8, 790, 836
AluI	AGCT	3 cut(s)	8, 790, 836
Alw26I	GTCTC	2 cut(s)	32, 829
AlwI	GGATC	3 cut(s)	91, 188, 417
AoxI	GGCC	4 cut(s)	363, 435, 560, 650
ApeKI	GCWGC	3 cut(s)	485, 764, 833
ApoI	RAATTY	4 cut(s)	139, 466, 718, 727
Asp700I	GAANNNNTTC	1 cut(s)	390
AspA2I	CCTAGG	1 cut(s)	610
AspLEI	GCGC	1 cut(s)	638
AspS9I	GGNCC	4 cut(s)	300, 560, 643, 931
AsuHPI	GGTGA	2 cut(s)	80, 535
AsuII	TTCGAA	1 cut(s)	665
AvaII	GGWCC	3 cut(s)	300, 643, 931
AvrII	CCTAGG	1 cut(s)	610
BbsI	GAAGAC	1 cut(s)	171
BbvI	GCAGC	3 cut(s)	472, 776, 845
BccI	CCATC	4 cut(s)	269, 355, 571, 833
BciT130I	CCWGG	1 cut(s)	913
BcoDI	GTCTC	2 cut(s)	32, 829
BfaI	CTAG	3 cut(s)	321, 374, 611
BfmI	CTRYAG	2 cut(s)	19, 849
BfoI	RGCGCY	1 cut(s)	639
BfuAI	ACCTGC	1 cut(s)	912
BisI	GCNGC	4 cut(s)	486, 653, 765, 834
BlnI	CCTAGG	1 cut(s)	610
BlsI	GCNGC	4 cut(s)	487, 654, 766, 835
Bme1390I	CCNGG	1 cut(s)	913
Bme18I	GGWCC	3 cut(s)	300, 643, 931
BmeRI	GACNNNNNGTC	2 cut(s)	49, 61
BmgT120I	GGNCC	4 cut(s)	300, 560, 643, 931
BmrFI	CCNGG	1 cut(s)	913
BmsI	GCATC	1 cut(s)	259
BoxI	GACNNNNGTC	2 cut(s)	44, 176
BpiI	GAAGAC	1 cut(s)	171
Bpu14I	TTCGAA	1 cut(s)	665
BpuEI	CTTGAG	1 cut(s)	920
Bsa29I	ATCGAT	1 cut(s)	117
BsaBI	GATNNNNATC	1 cut(s)	882
BsaHI	GRCGYC	1 cut(s)	45
BsaJI	CCNNGG	3 cut(s)	546, 610, 639
BsaWI	WCCGGW	1 cut(s)	168
Bsc4I	CCNNNNNNNGG	3 cut(s)	60, 64, 481
Bse118I	RCCGGY	1 cut(s)	369
Bse1I	ACTGG	1 cut(s)	411
Bse8I	GATNNNNATC	1 cut(s)	882
BseBI	CCWGG	1 cut(s)	913
BseCI	ATCGAT	1 cut(s)	117
BseDI	CCNNGG	3 cut(s)	546, 610, 639
BseJI	GATNNNNATC	1 cut(s)	882
BseLI	CCNNNNNNNGG	3 cut(s)	60, 64, 481
BseNI	ACTGG	1 cut(s)	411
BseXI	GCAGC	3 cut(s)	472, 776, 845
BsgI	GTGCAG	1 cut(s)	64
Bsh1236I	CGCG	3 cut(s)	177, 526, 655
Bsh1285I	CGRYCG	1 cut(s)	370
BshFI	GGCC	4 cut(s)	365, 437, 562, 652
BshVI	ATCGAT	1 cut(s)	117
BsiEI	CGRYCG	1 cut(s)	370
BsiSI	CCGG	2 cut(s)	169, 370
BslFI	GGGAC	2 cut(s)	38, 868
BslI	CCNNNNNNNGG	3 cut(s)	60, 64, 481
BsmAI	GTCTC	2 cut(s)	32, 829
BsmFI	GGGAC	2 cut(s)	38, 868
BsnI	GGCC	4 cut(s)	365, 437, 562, 652
Bsp119I	TTCGAA	1 cut(s)	665
Bsp1407I	TGTACA	1 cut(s)	774
Bsp143I	GATC	5 cut(s)	96, 148, 193, 409, 627
Bsp19I	CCATGG	2 cut(s)	546, 639
BspACI	CCGC	3 cut(s)	109, 653, 767
BspANI	GGCC	4 cut(s)	365, 437, 562, 652
BspDI	ATCGAT	1 cut(s)	117
BspFNI	CGCG	3 cut(s)	177, 526, 655
BspMI	ACCTGC	1 cut(s)	912
BspPI	GGATC	3 cut(s)	91, 188, 417
BspT104I	TTCGAA	1 cut(s)	665
BsrFI	RCCGGY	1 cut(s)	369
BsrGI	TGTACA	1 cut(s)	774
BsrI	ACTGG	1 cut(s)	411
BssAI	RCCGGY	1 cut(s)	369
BssECI	CCNNGG	3 cut(s)	546, 610, 639
BssMI	GATC	5 cut(s)	96, 148, 193, 409, 627
BssNI	GRCGYC	1 cut(s)	45
BssT1I	CCWWGG	3 cut(s)	546, 610, 639
Bst2UI	CCWGG	1 cut(s)	913
Bst4CI	ACNGT	1 cut(s)	809
Bst6I	CTCTTC	3 cut(s)	351, 588, 596
BstACI	GRCGYC	1 cut(s)	45
BstAUI	TGTACA	1 cut(s)	774
BstBI	TTCGAA	1 cut(s)	665
BstC8I	GCNNGC	4 cut(s)	32, 109, 490, 501
BstDSI	CCRYGG	2 cut(s)	546, 639
BstFNI	CGCG	3 cut(s)	177, 526, 655
BstH2I	RGCGCY	1 cut(s)	639
BstHHI	GCGC	1 cut(s)	638
BstKTI	GATC	5 cut(s)	99, 151, 196, 412, 630
BstMAI	GTCTC	2 cut(s)	32, 829
BstMBI	GATC	5 cut(s)	96, 148, 193, 409, 627
BstMCI	CGRYCG	1 cut(s)	370
BstNI	CCWGG	1 cut(s)	913
BstPAI	GACNNNNGTC	2 cut(s)	44, 176
BstSCI	CCNGG	1 cut(s)	911
BstSFI	CTRYAG	2 cut(s)	19, 849
BstUI	CGCG	3 cut(s)	177, 526, 655
BstV1I	GCAGC	3 cut(s)	472, 776, 845
BstV2I	GAAGAC	1 cut(s)	171
Bsu15I	ATCGAT	1 cut(s)	117
BsuRI	GGCC	4 cut(s)	365, 437, 562, 652
BsuTUI	ATCGAT	1 cut(s)	117
BtgI	CCRYGG	2 cut(s)	546, 639
BveI	ACCTGC	1 cut(s)	912
Cac8I	GCNNGC	4 cut(s)	32, 109, 490, 501
CfoI	GCGC	1 cut(s)	638
Cfr10I	RCCGGY	1 cut(s)	369
Cfr13I	GGNCC	4 cut(s)	300, 560, 643, 931
ClaI	ATCGAT	1 cut(s)	117
CseI	GACGC	1 cut(s)	166
CsiI	ACCWGGT	1 cut(s)	911
Csp6I	GTAC	3 cut(s)	264, 349, 775
CviAII	CATG	4 cut(s)	413, 547, 640, 778
CviQI	GTAC	3 cut(s)	264, 349, 775
DpnI	GATC	5 cut(s)	98, 150, 195, 411, 629
DpnII	GATC	5 cut(s)	96, 148, 193, 409, 627
DriI	GACNNNNNGTC	2 cut(s)	49, 61
EaeI	YGGCCR	2 cut(s)	363, 650
Eam1104I	CTCTTC	3 cut(s)	351, 588, 596
Eam1105I	GACNNNNNGTC	2 cut(s)	49, 61
EarI	CTCTTC	3 cut(s)	351, 588, 596
Eco130I	CCWWGG	3 cut(s)	546, 610, 639
Eco47I	GGWCC	3 cut(s)	300, 643, 931
Eco57I	CTGAAG	2 cut(s)	180, 734
EcoO109I	RGGNCCY	1 cut(s)	931
EcoRII	CCWGG	1 cut(s)	911
EcoT14I	CCWWGG	3 cut(s)	546, 610, 639
EcoT22I	ATGCAT	1 cut(s)	404
ErhI	CCWWGG	3 cut(s)	546, 610, 639
FaeI	CATG	4 cut(s)	416, 550, 643, 781
FaqI	GGGAC	2 cut(s)	38, 868
FatI	CATG	4 cut(s)	412, 546, 639, 777
FauI	CCCGC	1 cut(s)	116
Fnu4HI	GCNGC	4 cut(s)	486, 653, 765, 834
Fsp4HI	GCNGC	4 cut(s)	486, 653, 765, 834
FspBI	CTAG	3 cut(s)	321, 374, 611
GlaI	GCGC	1 cut(s)	637
GluI	GCNGC	4 cut(s)	486, 653, 765, 834
HaeII	RGCGCY	1 cut(s)	639
HaeIII	GGCC	4 cut(s)	365, 437, 562, 652
HapII	CCGG	2 cut(s)	169, 370
HgaI	GACGC	1 cut(s)	166
HhaI	GCGC	1 cut(s)	638
Hin1I	GRCGYC	1 cut(s)	45
Hin1II	CATG	4 cut(s)	416, 550, 643, 781
Hin6I	GCGC	1 cut(s)	636
HinP1I	GCGC	1 cut(s)	636
HinfI	GANTC	2 cut(s)	172, 667
HpaII	CCGG	2 cut(s)	169, 370
HphI	GGTGA	2 cut(s)	80, 535
Hpy166II	GTNNAC	1 cut(s)	316
Hpy188I	TCNGA	5 cut(s)	55, 153, 182, 193, 396
Hpy188III	TCNNGA	2 cut(s)	818, 937
Hpy8I	GTNNAC	1 cut(s)	316
Hpy99I	CGWCG	2 cut(s)	50, 182
HpyAV	CCTTC	5 cut(s)	94, 253, 380, 427, 688
HpyCH4III	ACNGT	1 cut(s)	809
HpyCH4IV	ACGT	1 cut(s)	45
HpyCH4V	TGCA	4 cut(s)	30, 81, 402, 921
HpySE526I	ACGT	1 cut(s)	45
Hsp92I	GRCGYC	1 cut(s)	45
Hsp92II	CATG	4 cut(s)	416, 550, 643, 781
HspAI	GCGC	1 cut(s)	636
Kzo9I	GATC	5 cut(s)	96, 148, 193, 409, 627
Lsp1109I	GCAGC	3 cut(s)	472, 776, 845
LweI	GCATC	1 cut(s)	259
MabI	ACCWGGT	1 cut(s)	911
MaeI	CTAG	3 cut(s)	321, 374, 611
MaeII	ACGT	1 cut(s)	45
MaeIII	GTNAC	1 cut(s)	68
MalI	GATC	5 cut(s)	98, 150, 195, 411, 629
MboI	GATC	5 cut(s)	96, 148, 193, 409, 627
MboII	GAAGA	9 cut(s)	173, 176, 256, 368, 521, 605, 613, 716, 727
MluCI	AATT	7 cut(s)	139, 280, 325, 466, 718, 727, 751
MlyI	GAGTC	1 cut(s)	166
MmeI	TCCRAC	3 cut(s)	78, 160, 906
MnlI	CCTC	7 cut(s)	110, 199, 297, 362, 503, 922, 944
Mph1103I	ATGCAT	1 cut(s)	404
MroXI	GAANNNNTTC	1 cut(s)	390
MspA1I	CMGCKG	2 cut(s)	767, 836
MspI	CCGG	2 cut(s)	169, 370
MspR9I	CCNGG	1 cut(s)	913
MvaI	CCWGG	1 cut(s)	913
MvnI	CGCG	3 cut(s)	177, 526, 655
NcoI	CCATGG	2 cut(s)	546, 639
NdeII	GATC	5 cut(s)	96, 148, 193, 409, 627
NlaIII	CATG	4 cut(s)	416, 550, 643, 781
NmeAIII	GCCGAG	1 cut(s)	154
NmuCI	GTSAC	1 cut(s)	68
NsiI	ATGCAT	1 cut(s)	404
NspV	TTCGAA	1 cut(s)	665
PcsI	WCGNNNNNNNCGW	1 cut(s)	51
PdmI	GAANNNNTTC	1 cut(s)	390
PfeI	GAWTC	1 cut(s)	667
PkrI	GCNGC	4 cut(s)	487, 654, 766, 835
PleI	GAGTC	1 cut(s)	166
PpsI	GAGTC	1 cut(s)	166
PpuMI	RGGWCCY	1 cut(s)	931
PshAI	GACNNNNGTC	2 cut(s)	44, 176
Psp5II	RGGWCCY	1 cut(s)	931
Psp6I	CCWGG	1 cut(s)	911
PspGI	CCWGG	1 cut(s)	911
PspPI	GGNCC	4 cut(s)	300, 560, 643, 931
PspPPI	RGGWCCY	1 cut(s)	931
PvuII	CAGCTG	1 cut(s)	836
RsaI	GTAC	3 cut(s)	265, 350, 776
RsaNI	GTAC	3 cut(s)	264, 349, 775
SatI	GCNGC	4 cut(s)	486, 653, 765, 834
Sau3AI	GATC	5 cut(s)	96, 148, 193, 409, 627
Sau96I	GGNCC	4 cut(s)	300, 560, 643, 931
SchI	GAGTC	1 cut(s)	166
ScrFI	CCNGG	1 cut(s)	913
SexAI	ACCWGGT	1 cut(s)	911
SfaNI	GCATC	1 cut(s)	259
SfcI	CTRYAG	2 cut(s)	19, 849
SfuI	TTCGAA	1 cut(s)	665
SinI	GGWCC	3 cut(s)	300, 643, 931
SmlI	CTYRAG	1 cut(s)	935
SmoI	CTYRAG	1 cut(s)	935
Sse9I	AATT	7 cut(s)	139, 280, 325, 466, 718, 727, 751
SsiI	CCGC	3 cut(s)	109, 653, 767
SspMI	CTAG	3 cut(s)	321, 374, 611
StyD4I	CCNGG	1 cut(s)	911
StyI	CCWWGG	3 cut(s)	546, 610, 639
TaaI	ACNGT	1 cut(s)	809
TaiI	ACGT	1 cut(s)	48
TaqI	TCGA	4 cut(s)	102, 117, 354, 665
TasI	AATT	7 cut(s)	139, 280, 325, 466, 718, 727, 751
TatI	WGTACW	1 cut(s)	774
TauI	GCSGC	1 cut(s)	655
TfiI	GAWTC	1 cut(s)	667
TseFI	GTSAC	1 cut(s)	68
TseI	GCWGC	3 cut(s)	485, 764, 833
Tsp45I	GTSAC	1 cut(s)	68
TspDTI	ATGAA	2 cut(s)	135, 602
TspGWI	ACGGA	1 cut(s)	186
VpaK11BI	GGWCC	3 cut(s)	300, 643, 931
XapI	RAATTY	4 cut(s)	139, 466, 718, 727
XmaJI	CCTAGG	1 cut(s)	610
XmnI	GAANNNNTTC	1 cut(s)	390
XspI	CTAG	3 cut(s)	321, 374, 611
ZraI	GACGTC	1 cut(s)	46
Zsp2I	ATGCAT	1 cut(s)	404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.