Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_111

Orthogroup ID

OG0024299

Physical Location & Seq

Genomic Coordinates Reverse (-)

3529689 .. 3533200

Gene Structure 3512 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom111g05530.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1674 bp

ATGGGACCTGAGTCACCAAGGTCCTTGTCCTTGTCCTGGGAACCCAGAGAAGTTGTGATCGACCACCAGTCGGGTGACACAGTCTGCACCGACTGTGGTTTGGTTCTTGGAGACCACTGCGTCGACGTCACCCCCAAGCGGCGAAAGTTTCATGATCAGAACCCCGGGGAAGACCGAAGTCGCGTGGGCGCTTCCTTGGATACTTTGCTGGACAATGGTGTTCTTACCACTTGTATCTCCAAAGACAGGAAGCAAGACAAGAAGAAGGATAATGTCGATGGCATTGCTCCTCCTAGTAACCAGTTGAACAACGCCGTGTCAAACCCCCAAAAATCTCTCGCAAAAGCTACCAAATACCTCGAATCCATGGCTTGTTGGCTAGACAAACATGGCCTTCCCGATCAGGTTCTACATGATGCCGAAGCGTTATACAAGAAGGCCGTTGATAAGAAGATTCGTAGTGGGAGAAAGTTTTGTAGGGGGAGAAAGTTTGAGGTTATGGTGGCCGCTTGCCTCTTTCTTGCTTGCCAAGAAAACAATAACTCGCGAACACCCAAGGAAATCGCCAAGGCTGCCAACGGACCTTCTAGGAAAGAAATTAACAAAATGGTAAAAGTCTTGGAGAAAGAGCTAGAGATCCGTGTTAGAACCCTGGCTTTACATCCCACAATACAAAACATAAGTTATTCCGCAAATCTCTCTCTCTCTGTGAAAATCATGGAAGATAAAGAGTTCTGTAGAGACTGCAAGCAAGAGACAGACGTCGTGTCCGACCACACGTCGGGTGACACAATCTGCACCGTCTGTGGTTTGATCCTTGAAGCCCGCTGCATCGATGTTACCCCCGAGTGGCGAAATTTTGCCGATCAGAACCCCGAAGAAGACCGGACTCGCGTCGGAAAAGTCTCCGATCCTTTGCTGGATAATGGTGTTCTTTCCACTTGTATATCCAAAGAAAAGAAGAAAAACACCAAGAAGGGTACTGATGCCGATGGTAATTTTCCTCCTAATAAATGGACCAATAGGGCTGTAAACCCTTACAATTCTCTCCTAAAACCTTTCAAGTACCTCGAAGAGATGGCCGCCCGGCTAGACATATACGAAGGCGTTCCGAAATGCATACTGGATCATGCCAGAGGTCTATACATGAAGGCCGATGATAAGAACTTCTGTAAGGGGAGAAATTCTAACCCTATAATGGCTGCTTGCCTCTTGCTTGCATTTGAAGAAAGCCAAAATACGCGAACACTAAAGGAAATCACCATGGTTGCCAATGGGCCATCTAAAAAAGAAATTAGCAAGATGAAGGAAGAGTTGAAGAGAAGACTAGGGCTTGCTTCCAAGATCAAAAGCGCCATCGACCTTTGGCCGCGACATTGCTCGAATCTTGGTATGAGTAGTAAAGATAAGAAGGCTGTGGAAGAAGCTCTGAAGAATTTGGCAAATTTTGATATTAGGAGAAACCCCAATTCTGTTGTGGCAGCGGTTATGTACATGGTAGTTCAGCTTTCTAACGGGAATAACTGTTCTGTTCAAGATGTCTCAAAAGCAGCTGATGGTGTTGCTGTAGGGACAACTAAAAAGACATACAAGGATATTTATCCCTATGCTTCACAGATAATACCAACCTGGTATTGCAGGTTGGAGGACCTCAAGAAACTTATCACTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000769 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006949 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009960 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046686 GO:0048316 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050896 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko03022 ko05169 ko05203 map03022 map05169 map05203

Pfam Domains

Protein Families

TFIIB TF_Zn_Ribbon

Protein Analysis

558

Amino Acids

62.24

Weight (kDa)

8.94

Isoelectric Point (pI)

35.3

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0024299)

Species	Orthologous Gene IDs
malus_domestica	MD09G1065500.v1.1 MD09G1065600.v1.1
pyrus_communis	pycom111g05530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	119
AatII	GACGTC	2 cut(s)	129, 765
Acc36I	ACCTGC	1 cut(s)	1629
AccI	GTMKAC	1 cut(s)	123
AccII	CGCG	5 cut(s)	183, 547, 894, 1243, 1372
AciI	CCGC	7 cut(s)	139, 507, 690, 826, 1083, 1370, 1484
AclWI	GGATC	4 cut(s)	631, 808, 905, 1134
AcoI	YGGCCR	3 cut(s)	504, 1080, 1367
AcsI	RAATTY	4 cut(s)	856, 1183, 1435, 1444
AcuI	CTGAAG	1 cut(s)	1451
AcyI	GRCGYC	2 cut(s)	126, 762
AfaI	GTAC	3 cut(s)	982, 1067, 1493
AfiI	CCNNNNNNNGG	7 cut(s)	36, 70, 138, 246, 781, 849, 1198
AflIII	ACRYGT	1 cut(s)	777
AgsI	TTSAA	6 cut(s)	307, 821, 1063, 1226, 1318, 1535
AhdI	GACNNNNNGTC	3 cut(s)	67, 766, 778
AjiI	CACGTC	1 cut(s)	780
AjnI	CCWGG	3 cut(s)	35, 651, 1628
AloI	GAACNNNNNNTCC	2 cut(s)	1510, 1542
AluBI	AGCT	5 cut(s)	347, 631, 1427, 1507, 1553
AluI	AGCT	5 cut(s)	347, 631, 1427, 1507, 1553
Alw26I	GTCTC	5 cut(s)	105, 735, 749, 910, 1546
AlwI	GGATC	4 cut(s)	631, 808, 905, 1134
Ama87I	CYCGRG	2 cut(s)	164, 845
AoxI	GGCC	7 cut(s)	391, 438, 504, 1080, 1152, 1277, 1367
ApeKI	GCWGC	5 cut(s)	572, 828, 1202, 1481, 1550
ApoI	RAATTY	4 cut(s)	856, 1183, 1435, 1444
Asp700I	GAANNNNTTC	1 cut(s)	1107
AspLEI	GCGC	2 cut(s)	191, 1355
AspS9I	GGNCC	6 cut(s)	5, 21, 581, 1017, 1277, 1648
AsuC2I	CCSGG	3 cut(s)	165, 166, 1087
AsuHPI	GGTGA	5 cut(s)	6, 86, 121, 797, 1252
AvaI	CYCGRG	2 cut(s)	164, 845
AvaII	GGWCC	5 cut(s)	5, 21, 581, 1017, 1648
BbsI	GAAGAC	3 cut(s)	177, 888, 1330
BbvI	GCAGC	5 cut(s)	559, 815, 1189, 1493, 1562
BccI	CCATC	6 cut(s)	272, 986, 1072, 1288, 1364, 1550
BceAI	ACGGC	2 cut(s)	299, 425
BcgI	CGANNNNNNTGC	2 cut(s)	266, 300
BciT130I	CCWGG	3 cut(s)	37, 653, 1630
BciVI	GTATCC	1 cut(s)	193
BclI	TGATCA	1 cut(s)	154
BcnI	CCSGG	3 cut(s)	165, 166, 1087
BcoDI	GTCTC	5 cut(s)	105, 735, 749, 910, 1546
BfaI	CTAG	6 cut(s)	294, 380, 588, 632, 1091, 1328
BfmI	CTRYAG	2 cut(s)	736, 1566
BfoI	RGCGCY	2 cut(s)	192, 1356
BfuAI	ACCTGC	1 cut(s)	1629
BfuI	GTATCC	1 cut(s)	193
BisI	GCNGC	9 cut(s)	140, 507, 573, 829, 1083, 1203, 1370, 1482, 1551
BlsI	GCNGC	9 cut(s)	141, 508, 574, 830, 1084, 1204, 1371, 1483, 1552
Bme1390I	CCNGG	6 cut(s)	37, 165, 166, 653, 1087, 1630
Bme18I	GGWCC	5 cut(s)	5, 21, 581, 1017, 1648
BmeRI	GACNNNNNGTC	3 cut(s)	67, 766, 778
BmeT110I	CYCGRG	2 cut(s)	164, 845
BmgBI	CACGTC	1 cut(s)	780
BmgT120I	GGNCC	6 cut(s)	5, 21, 581, 1017, 1277, 1648
BmiI	GGNNCC	2 cut(s)	6, 42
BmrFI	CCNGG	6 cut(s)	37, 165, 166, 653, 1087, 1630
BmsI	GCATC	3 cut(s)	406, 840, 976
BoxI	GACNNNNGTC	4 cut(s)	10, 177, 761, 893
BpiI	GAAGAC	3 cut(s)	177, 888, 1330
BpuEI	CTTGAG	1 cut(s)	1637
BpuMI	CCSGG	3 cut(s)	165, 166, 1087
Bsa29I	ATCGAT	1 cut(s)	834
BsaBI	GATNNNNATC	1 cut(s)	1599
BsaHI	GRCGYC	2 cut(s)	126, 762
BsaI	GGTCTC	1 cut(s)	105
BsaWI	WCCGGW	1 cut(s)	885
Bsc4I	CCNNNNNNNGG	7 cut(s)	36, 70, 138, 246, 781, 849, 1198
Bse1I	ACTGG	3 cut(s)	67, 301, 1128
Bse3DI	GCAATG	2 cut(s)	282, 1375
Bse8I	GATNNNNATC	1 cut(s)	1599
BseBI	CCWGG	3 cut(s)	37, 653, 1630
BseCI	ATCGAT	1 cut(s)	834
BseGI	GGATG	1 cut(s)	661
BseJI	GATNNNNATC	1 cut(s)	1599
BseLI	CCNNNNNNNGG	7 cut(s)	36, 70, 138, 246, 781, 849, 1198
BseMI	GCAATG	2 cut(s)	282, 1375
BseNI	ACTGG	3 cut(s)	67, 301, 1128
BseRI	GAGGAG	1 cut(s)	279
BseXI	GCAGC	5 cut(s)	559, 815, 1189, 1493, 1562
BsgI	GTGCAG	2 cut(s)	70, 781
Bsh1236I	CGCG	5 cut(s)	183, 547, 894, 1243, 1372
BshFI	GGCC	7 cut(s)	393, 440, 506, 1082, 1154, 1279, 1369
BshVI	ATCGAT	1 cut(s)	834
BsiHKCI	CYCGRG	2 cut(s)	164, 845
BsiSI	CCGG	3 cut(s)	165, 886, 1087
BslFI	GGGAC	2 cut(s)	18, 1585
BslI	CCNNNNNNNGG	7 cut(s)	36, 70, 138, 246, 781, 849, 1198
BsmAI	GTCTC	5 cut(s)	105, 735, 749, 910, 1546
BsmFI	GGGAC	2 cut(s)	18, 1585
BsnI	GGCC	7 cut(s)	393, 440, 506, 1082, 1154, 1279, 1369
Bso31I	GGTCTC	1 cut(s)	105
BsoBI	CYCGRG	2 cut(s)	164, 845
Bsp1407I	TGTACA	1 cut(s)	1491
Bsp143I	GATC	9 cut(s)	57, 154, 400, 636, 813, 865, 910, 1126, 1344
Bsp19I	CCATGG	2 cut(s)	366, 1263
Bsp68I	TCGCGA	1 cut(s)	547
BspACI	CCGC	7 cut(s)	139, 507, 690, 826, 1083, 1370, 1484
BspANI	GGCC	7 cut(s)	393, 440, 506, 1082, 1154, 1279, 1369
BspDI	ATCGAT	1 cut(s)	834
BspFNI	CGCG	5 cut(s)	183, 547, 894, 1243, 1372
BspHI	TCATGA	1 cut(s)	151
BspLI	GGNNCC	2 cut(s)	6, 42
BspMI	ACCTGC	1 cut(s)	1629
BspPI	GGATC	4 cut(s)	631, 808, 905, 1134
BspTNI	GGTCTC	1 cut(s)	105
BsrDI	GCAATG	2 cut(s)	282, 1375
BsrGI	TGTACA	1 cut(s)	1491
BsrI	ACTGG	3 cut(s)	67, 301, 1128
BssMI	GATC	9 cut(s)	57, 154, 400, 636, 813, 865, 910, 1126, 1344
BssNI	GRCGYC	2 cut(s)	126, 762
BssT1I	CCWWGG	6 cut(s)	17, 195, 366, 555, 567, 1263
Bst2UI	CCWGG	3 cut(s)	37, 653, 1630
Bst4CI	ACNGT	4 cut(s)	82, 95, 802, 1526
Bst6I	CTCTTC	3 cut(s)	1068, 1305, 1313
BstACI	GRCGYC	2 cut(s)	126, 762
BstAUI	TGTACA	1 cut(s)	1491
BstC8I	GCNNGC	7 cut(s)	511, 526, 749, 826, 1207, 1218, 1335
BstDEI	CTNAG	1 cut(s)	9
BstDSI	CCRYGG	2 cut(s)	366, 1263
BstF5I	GGATG	1 cut(s)	661
BstFNI	CGCG	5 cut(s)	183, 547, 894, 1243, 1372
BstH2I	RGCGCY	2 cut(s)	192, 1356
BstHHI	GCGC	2 cut(s)	191, 1355
BstKTI	GATC	9 cut(s)	60, 157, 403, 639, 816, 868, 913, 1129, 1347
BstMAI	GTCTC	5 cut(s)	105, 735, 749, 910, 1546
BstMBI	GATC	9 cut(s)	57, 154, 400, 636, 813, 865, 910, 1126, 1344
BstMWI	GCNNNNNNNGC	1 cut(s)	572
BstNI	CCWGG	3 cut(s)	37, 653, 1630
BstPAI	GACNNNNGTC	4 cut(s)	10, 177, 761, 893
BstSCI	CCNGG	6 cut(s)	35, 163, 164, 651, 1085, 1628
BstSFI	CTRYAG	2 cut(s)	736, 1566
BstUI	CGCG	5 cut(s)	183, 547, 894, 1243, 1372
BstV1I	GCAGC	5 cut(s)	559, 815, 1189, 1493, 1562
BstV2I	GAAGAC	3 cut(s)	177, 888, 1330
BstX2I	RGATCY	1 cut(s)	636
BstYI	RGATCY	1 cut(s)	636
Bsu15I	ATCGAT	1 cut(s)	834
BsuI	GTATCC	1 cut(s)	193
BsuRI	GGCC	7 cut(s)	393, 440, 506, 1082, 1154, 1279, 1369
BsuTUI	ATCGAT	1 cut(s)	834
BtgI	CCRYGG	2 cut(s)	366, 1263
BtrI	CACGTC	1 cut(s)	780
BtsCI	GGATG	1 cut(s)	661
BtsI	GCAGTG	1 cut(s)	115
BtsIMutI	CAGTG	1 cut(s)	115
BtuMI	TCGCGA	1 cut(s)	547
BveI	ACCTGC	1 cut(s)	1629
Cac8I	GCNNGC	7 cut(s)	511, 526, 749, 826, 1207, 1218, 1335
CciI	TCATGA	1 cut(s)	151
CfoI	GCGC	2 cut(s)	191, 1355
Cfr13I	GGNCC	6 cut(s)	5, 21, 581, 1017, 1277, 1648
Cfr9I	CCCGGG	1 cut(s)	164
ClaI	ATCGAT	1 cut(s)	834
CseI	GACGC	2 cut(s)	109, 883
CsiI	ACCWGGT	1 cut(s)	1628
Csp6I	GTAC	3 cut(s)	981, 1066, 1492
CviAII	CATG	9 cut(s)	152, 367, 389, 413, 718, 1130, 1147, 1264, 1495
CviQI	GTAC	3 cut(s)	981, 1066, 1492
DdeI	CTNAG	1 cut(s)	9
DpnI	GATC	9 cut(s)	59, 156, 402, 638, 815, 867, 912, 1128, 1346
DpnII	GATC	9 cut(s)	57, 154, 400, 636, 813, 865, 910, 1126, 1344
DrdI	GACNNNNNNGTC	1 cut(s)	119
DriI	GACNNNNNGTC	3 cut(s)	67, 766, 778
DseDI	GACNNNNNNGTC	1 cut(s)	119
EaeI	YGGCCR	3 cut(s)	504, 1080, 1367
Eam1104I	CTCTTC	3 cut(s)	1068, 1305, 1313
Eam1105I	GACNNNNNGTC	3 cut(s)	67, 766, 778
EarI	CTCTTC	3 cut(s)	1068, 1305, 1313
Eco130I	CCWWGG	6 cut(s)	17, 195, 366, 555, 567, 1263
Eco31I	GGTCTC	1 cut(s)	105
Eco47I	GGWCC	5 cut(s)	5, 21, 581, 1017, 1648
Eco57I	CTGAAG	1 cut(s)	1451
Eco88I	CYCGRG	2 cut(s)	164, 845
EcoO109I	RGGNCCY	3 cut(s)	5, 21, 1648
EcoRII	CCWGG	3 cut(s)	35, 651, 1628
EcoT14I	CCWWGG	6 cut(s)	17, 195, 366, 555, 567, 1263
EcoT22I	ATGCAT	1 cut(s)	1121
ErhI	CCWWGG	6 cut(s)	17, 195, 366, 555, 567, 1263
FaeI	CATG	9 cut(s)	155, 370, 392, 416, 721, 1133, 1150, 1267, 1498
FaqI	GGGAC	2 cut(s)	18, 1585
FatI	CATG	9 cut(s)	151, 366, 388, 412, 717, 1129, 1146, 1263, 1494
FauI	CCCGC	1 cut(s)	833
FbaI	TGATCA	1 cut(s)	154
FblI	GTMKAC	1 cut(s)	123
Fnu4HI	GCNGC	9 cut(s)	140, 507, 573, 829, 1083, 1203, 1370, 1482, 1551
FokI	GGATG	1 cut(s)	648
Fsp4HI	GCNGC	9 cut(s)	140, 507, 573, 829, 1083, 1203, 1370, 1482, 1551
FspBI	CTAG	6 cut(s)	294, 380, 588, 632, 1091, 1328
GlaI	GCGC	2 cut(s)	190, 1354
GluI	GCNGC	9 cut(s)	140, 507, 573, 829, 1083, 1203, 1370, 1482, 1551
HaeII	RGCGCY	2 cut(s)	192, 1356
HaeIII	GGCC	7 cut(s)	393, 440, 506, 1082, 1154, 1279, 1369
HapII	CCGG	3 cut(s)	165, 886, 1087
HgaI	GACGC	2 cut(s)	109, 883
HhaI	GCGC	2 cut(s)	191, 1355
Hin1I	GRCGYC	2 cut(s)	126, 762
Hin1II	CATG	9 cut(s)	155, 370, 392, 416, 721, 1133, 1150, 1267, 1498
Hin6I	GCGC	2 cut(s)	189, 1353
HinP1I	GCGC	2 cut(s)	189, 1353
HincII	GTYRAC	1 cut(s)	124
HindII	GTYRAC	1 cut(s)	124
HinfI	GANTC	5 cut(s)	11, 362, 454, 889, 1384
HpaII	CCGG	3 cut(s)	165, 886, 1087
HphI	GGTGA	5 cut(s)	6, 86, 121, 797, 1252
Hpy166II	GTNNAC	2 cut(s)	124, 1033
Hpy188I	TCNGA	7 cut(s)	159, 772, 870, 899, 910, 1113, 1431
Hpy188III	TCNNGA	5 cut(s)	152, 398, 546, 1535, 1654
Hpy8I	GTNNAC	2 cut(s)	124, 1033
Hpy99I	CGWCG	5 cut(s)	125, 128, 767, 784, 899
HpyAV	CCTTC	9 cut(s)	259, 404, 430, 594, 970, 1097, 1144, 1300, 1405
HpyCH4III	ACNGT	4 cut(s)	82, 95, 802, 1526
HpyCH4IV	ACGT	3 cut(s)	126, 762, 779
HpyCH4V	TGCA	7 cut(s)	87, 747, 798, 831, 1119, 1220, 1638
HpyF10VI	GCNNNNNNNGC	1 cut(s)	572
HpyF3I	CTNAG	1 cut(s)	9
HpySE526I	ACGT	3 cut(s)	126, 762, 779
Hsp92I	GRCGYC	2 cut(s)	126, 762
Hsp92II	CATG	9 cut(s)	155, 370, 392, 416, 721, 1133, 1150, 1267, 1498
HspAI	GCGC	2 cut(s)	189, 1353
Ksp22I	TGATCA	1 cut(s)	154
Kzo9I	GATC	9 cut(s)	57, 154, 400, 636, 813, 865, 910, 1126, 1344
LmnI	GCTCC	1 cut(s)	292
Lsp1109I	GCAGC	5 cut(s)	559, 815, 1189, 1493, 1562
LweI	GCATC	3 cut(s)	406, 840, 976
MabI	ACCWGGT	1 cut(s)	1628
MaeI	CTAG	6 cut(s)	294, 380, 588, 632, 1091, 1328
MaeII	ACGT	3 cut(s)	126, 762, 779
MaeIII	GTNAC	6 cut(s)	12, 74, 127, 296, 785, 838
MalI	GATC	9 cut(s)	59, 156, 402, 638, 815, 867, 912, 1128, 1346
MboI	GATC	9 cut(s)	57, 154, 400, 636, 813, 865, 910, 1126, 1344
MflI	RGATCY	1 cut(s)	636
MluCI	AATT	9 cut(s)	597, 856, 997, 1042, 1183, 1293, 1435, 1444, 1468
MlyI	GAGTC	2 cut(s)	20, 883
MmeI	TCCRAC	3 cut(s)	795, 877, 1623
Mph1103I	ATGCAT	1 cut(s)	1121
MroXI	GAANNNNTTC	1 cut(s)	1107
MseI	TTAA	1 cut(s)	600
MspA1I	CMGCKG	3 cut(s)	828, 1484, 1553
MspI	CCGG	3 cut(s)	165, 886, 1087
MspR9I	CCNGG	6 cut(s)	37, 165, 166, 653, 1087, 1630
MvaI	CCWGG	3 cut(s)	37, 653, 1630
MvnI	CGCG	5 cut(s)	183, 547, 894, 1243, 1372
MwoI	GCNNNNNNNGC	1 cut(s)	572
NciI	CCSGG	3 cut(s)	165, 166, 1087
NcoI	CCATGG	2 cut(s)	366, 1263
NdeII	GATC	9 cut(s)	57, 154, 400, 636, 813, 865, 910, 1126, 1344
NlaIII	CATG	9 cut(s)	155, 370, 392, 416, 721, 1133, 1150, 1267, 1498
NlaIV	GGNNCC	2 cut(s)	6, 42
NmuCI	GTSAC	4 cut(s)	12, 74, 127, 785
NruI	TCGCGA	1 cut(s)	547
NsiI	ATGCAT	1 cut(s)	1121
PagI	TCATGA	1 cut(s)	151
PcsI	WCGNNNNNNNCGW	1 cut(s)	768
PdmI	GAANNNNTTC	1 cut(s)	1107
PfeI	GAWTC	3 cut(s)	362, 454, 1384
PflFI	GACNNNGTC	1 cut(s)	80
PkrI	GCNGC	9 cut(s)	141, 508, 574, 830, 1084, 1204, 1371, 1483, 1552
PleI	GAGTC	2 cut(s)	19, 883
PpsI	GAGTC	2 cut(s)	19, 883
PpuMI	RGGWCCY	3 cut(s)	5, 21, 1648
PshAI	GACNNNNGTC	4 cut(s)	10, 177, 761, 893
Psp5II	RGGWCCY	3 cut(s)	5, 21, 1648
Psp6I	CCWGG	3 cut(s)	35, 651, 1628
PspGI	CCWGG	3 cut(s)	35, 651, 1628
PspN4I	GGNNCC	2 cut(s)	6, 42
PspPI	GGNCC	6 cut(s)	5, 21, 581, 1017, 1277, 1648
PspPPI	RGGWCCY	3 cut(s)	5, 21, 1648
PsuI	RGATCY	1 cut(s)	636
PsyI	GACNNNGTC	1 cut(s)	80
PvuII	CAGCTG	1 cut(s)	1553
RruI	TCGCGA	1 cut(s)	547
RsaI	GTAC	3 cut(s)	982, 1067, 1493
RsaNI	GTAC	3 cut(s)	981, 1066, 1492
SalI	GTCGAC	1 cut(s)	122
SaqAI	TTAA	1 cut(s)	600
SatI	GCNGC	9 cut(s)	140, 507, 573, 829, 1083, 1203, 1370, 1482, 1551
Sau3AI	GATC	9 cut(s)	57, 154, 400, 636, 813, 865, 910, 1126, 1344
Sau96I	GGNCC	6 cut(s)	5, 21, 581, 1017, 1277, 1648
SchI	GAGTC	2 cut(s)	20, 883
ScrFI	CCNGG	6 cut(s)	37, 165, 166, 653, 1087, 1630
SexAI	ACCWGGT	1 cut(s)	1628
SfaNI	GCATC	3 cut(s)	406, 840, 976
SfcI	CTRYAG	2 cut(s)	736, 1566
SgrDI	CGTCGACG	1 cut(s)	122
SinI	GGWCC	5 cut(s)	5, 21, 581, 1017, 1648
SmaI	CCCGGG	1 cut(s)	166
SmlI	CTYRAG	1 cut(s)	1652
SmoI	CTYRAG	1 cut(s)	1652
Sse9I	AATT	9 cut(s)	597, 856, 997, 1042, 1183, 1293, 1435, 1444, 1468
SsiI	CCGC	7 cut(s)	139, 507, 690, 826, 1083, 1370, 1484
SspMI	CTAG	6 cut(s)	294, 380, 588, 632, 1091, 1328
StyD4I	CCNGG	6 cut(s)	35, 163, 164, 651, 1085, 1628
StyI	CCWWGG	6 cut(s)	17, 195, 366, 555, 567, 1263
TaaI	ACNGT	4 cut(s)	82, 95, 802, 1526
TaiI	ACGT	3 cut(s)	129, 765, 782
TaqI	TCGA	8 cut(s)	60, 123, 276, 360, 834, 1071, 1359, 1382
TaqII	GACCGA	1 cut(s)	189
TasI	AATT	9 cut(s)	597, 856, 997, 1042, 1183, 1293, 1435, 1444, 1468
TatI	WGTACW	1 cut(s)	1491
TauI	GCSGC	4 cut(s)	142, 509, 1085, 1372
TfiI	GAWTC	3 cut(s)	362, 454, 1384
Tru1I	TTAA	1 cut(s)	600
Tru9I	TTAA	1 cut(s)	600
TscAI	CASTG	1 cut(s)	122
TseFI	GTSAC	4 cut(s)	12, 74, 127, 785
TseI	GCWGC	5 cut(s)	572, 828, 1202, 1481, 1550
Tsp45I	GTSAC	4 cut(s)	12, 74, 127, 785
TspDTI	ATGAA	3 cut(s)	140, 1163, 1319
TspGWI	ACGGA	2 cut(s)	594, 629
TspMI	CCCGGG	1 cut(s)	164
TspRI	CASTG	1 cut(s)	122
Tth111I	GACNNNGTC	1 cut(s)	80
VpaK11BI	GGWCC	5 cut(s)	5, 21, 581, 1017, 1648
XapI	RAATTY	4 cut(s)	856, 1183, 1435, 1444
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1474
XmaI	CCCGGG	1 cut(s)	164
XmiI	GTMKAC	1 cut(s)	123
XmnI	GAANNNNTTC	1 cut(s)	1107
XspI	CTAG	6 cut(s)	294, 380, 588, 632, 1091, 1328
ZraI	GACGTC	2 cut(s)	127, 763
Zsp2I	ATGCAT	1 cut(s)	1121

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.