MD09G1107500.v1.1

Transmembrane protein 97-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0024307
Physical Location & Seq
Reverse (-)
7845638 .. 7847105
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1107500.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGGTGTTTTTGTGAAGATAATCGACGGGTTTCTCTTTGTGAATTTTGCCATGGCGGCGGTGTGCGCGGTGTTGATAGATACACAGTTGTGTCTGCCTCCAAGCCTCTTCCCAAGCTCGGTGGTGGAGATCAAGAACTGGTATGCTCGCGAGTTCGATGATTATCTCGTTGAACAGAAGCCTCATTTCTTGGTTGGCCTCATTTGGGTTGAGCTTATATTGCAGTGGCCTCTTCTCGTTGCCAACTTGTATGCAATTTTGACTGCTAAGCCTTGGTTCAACACCACCTGCTTGGTCTATGGCGTCTCTTTCTTCACTACCATGGGCGCTGTATTAACAGAGATGTTAAGGTCCGGCAAGGCATCTAAGAAAATGTTGAATGTGCACTACGCTGCTATGGGGTTGGCTGTTCTGTCCATACTGCGCGGTCTGCTCCCACCGTCCGCTGCCGCTACTCCAACCAGCGGCAAGGGAATAACCAAAAGGACAGACCCACGAAAGAAAACGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2781

Protein Analysis

171

Amino Acids

18.8

Weight (kDa)

9.35

Isoelectric Point (pI)

36.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 43 - 120 2.3e-12 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024307)

Species Orthologous Gene IDs
malus_domestica MD09G1107500.v1.1 MD17G1095600.v1.1
prunus_persica Prupe.3G218200_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 296
Acc36I ACCTGC 1 cut(s) 296
AccII CGCG 3 cut(s) 68, 150, 426
AciI CCGC 7 cut(s) 56, 59, 68, 426, 444, 450, 465
AcsI RAATTY 1 cut(s) 43
AcyI GRCGYC 1 cut(s) 303
AfiI CCNNNNNNNGG 3 cut(s) 118, 205, 464
AgsI TTSAA 3 cut(s) 173, 280, 379
AleI CACNNNNGTG 1 cut(s) 88
AluBI AGCT 2 cut(s) 117, 214
AluI AGCT 2 cut(s) 117, 214
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 310
Alw44I GTGCAC 1 cut(s) 383
AoxI GGCC 2 cut(s) 196, 227
ApaLI GTGCAC 1 cut(s) 383
ApeKI GCWGC 2 cut(s) 392, 446
ApoI RAATTY 1 cut(s) 43
AspLEI GCGC 3 cut(s) 68, 329, 426
AspS9I GGNCC 1 cut(s) 351
AvaII GGWCC 1 cut(s) 351
BaeGI GKGCMC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 2 cut(s) 379, 433
BcoDI GTCTC 1 cut(s) 310
BfoI RGCGCY 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 296
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 5 cut(s) 57, 393, 447, 450, 466
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 5 cut(s) 58, 394, 448, 451, 467
Bme18I GGWCC 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 351
BmsI GCATC 1 cut(s) 371
Bpu1102I GCTNAGC 1 cut(s) 267
BsaBI GATNNNNATC 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 3 cut(s) 51, 272, 321
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 205, 464
Bse1I ACTGG 1 cut(s) 143
Bse8I GATNNNNATC 1 cut(s) 162
BseDI CCNNGG 3 cut(s) 51, 272, 321
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 3 cut(s) 118, 205, 464
BseNI ACTGG 1 cut(s) 143
BseSI GKGCMC 1 cut(s) 387
BseXI GCAGC 2 cut(s) 379, 433
Bsh1236I CGCG 3 cut(s) 68, 150, 426
BshFI GGCC 2 cut(s) 198, 229
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 1 cut(s) 354
BslI CCNNNNNNNGG 3 cut(s) 118, 205, 464
BsmAI GTCTC 1 cut(s) 310
BsmBI CGTCTC 1 cut(s) 310
BsnI GGCC 2 cut(s) 198, 229
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 1 cut(s) 129
Bsp1720I GCTNAGC 1 cut(s) 267
Bsp19I CCATGG 2 cut(s) 51, 321
Bsp68I TCGCGA 1 cut(s) 150
BspACI CCGC 7 cut(s) 56, 59, 68, 426, 444, 450, 465
BspANI GGCC 2 cut(s) 198, 229
BspFNI CGCG 3 cut(s) 68, 150, 426
BspMI ACCTGC 1 cut(s) 296
BsrI ACTGG 1 cut(s) 143
BssECI CCNNGG 3 cut(s) 51, 272, 321
BssMI GATC 1 cut(s) 129
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 3 cut(s) 51, 272, 321
Bst4CI ACNGT 2 cut(s) 87, 441
Bst6I CTCTTC 2 cut(s) 113, 237
BstACI GRCGYC 1 cut(s) 303
BstC8I GCNNGC 1 cut(s) 148
BstDEI CTNAG 2 cut(s) 267, 366
BstDSI CCRYGG 2 cut(s) 51, 321
BstFNI CGCG 3 cut(s) 68, 150, 426
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 3 cut(s) 68, 329, 426
BstKTI GATC 1 cut(s) 132
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 4 cut(s) 56, 65, 220, 430
BstSLI GKGCMC 1 cut(s) 387
BstUI CGCG 3 cut(s) 68, 150, 426
BstV1I GCAGC 2 cut(s) 379, 433
BstXI CCANNNNNNTGG 1 cut(s) 292
BsuRI GGCC 2 cut(s) 198, 229
BtgI CCRYGG 2 cut(s) 51, 321
BtsI GCAGTG 1 cut(s) 230
BtsIMutI CAGTG 1 cut(s) 230
BtuMI TCGCGA 1 cut(s) 150
BveI ACCTGC 1 cut(s) 296
Cac8I GCNNGC 1 cut(s) 148
CfoI GCGC 3 cut(s) 68, 329, 426
Cfr13I GGNCC 1 cut(s) 351
CseI GACGC 1 cut(s) 292
CspCI CAANNNNNGTGG 2 cut(s) 102, 137
CviAII CATG 2 cut(s) 52, 322
CviJI RGCY 8 cut(s) 105, 117, 181, 198, 214, 229, 271, 407
CviKI_1 RGCY 8 cut(s) 105, 117, 181, 198, 214, 229, 271, 407
DdeI CTNAG 2 cut(s) 267, 366
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 113, 237
EarI CTCTTC 2 cut(s) 113, 237
Eco130I CCWWGG 3 cut(s) 51, 272, 321
Eco47I GGWCC 1 cut(s) 351
EcoT14I CCWWGG 3 cut(s) 51, 272, 321
ErhI CCWWGG 3 cut(s) 51, 272, 321
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 2 cut(s) 55, 325
FaiI YATR 8 cut(s) 53, 144, 218, 252, 300, 323, 398, 419
FatI CATG 2 cut(s) 51, 321
Fnu4HI GCNGC 5 cut(s) 57, 393, 447, 450, 466
Fsp4HI GCNGC 5 cut(s) 57, 393, 447, 450, 466
GlaI GCGC 3 cut(s) 67, 328, 425
GluI GCNGC 5 cut(s) 57, 393, 447, 450, 466
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 2 cut(s) 198, 229
HapII CCGG 1 cut(s) 354
HgaI GACGC 1 cut(s) 292
HhaI GCGC 3 cut(s) 68, 329, 426
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 2 cut(s) 55, 325
Hin6I GCGC 3 cut(s) 66, 327, 424
HinP1I GCGC 3 cut(s) 66, 327, 424
HpaII CCGG 1 cut(s) 354
Hpy166II GTNNAC 1 cut(s) 385
Hpy188III TCNNGA 2 cut(s) 133, 149
Hpy8I GTNNAC 1 cut(s) 385
Hpy99I CGWCG 1 cut(s) 29
HpyCH4III ACNGT 2 cut(s) 87, 441
HpyCH4V TGCA 3 cut(s) 223, 254, 385
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 65, 220, 430
HpyF3I CTNAG 2 cut(s) 267, 366
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 2 cut(s) 55, 325
HspAI GCGC 3 cut(s) 66, 327, 424
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 1 cut(s) 438
LpnPI CCDG 4 cut(s) 124, 301, 367, 475
Lsp1109I GCAGC 2 cut(s) 379, 433
LweI GCATC 1 cut(s) 371
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 4 cut(s) 28, 100, 224, 304
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 2 cut(s) 43, 255
MmeI TCCRAC 1 cut(s) 482
MnlI CCTC 5 cut(s) 108, 116, 192, 209, 240
MseI TTAA 2 cut(s) 335, 347
MslI CAYNNNNRTG 2 cut(s) 88, 320
MspA1I CMGCKG 2 cut(s) 446, 465
MspI CCGG 1 cut(s) 354
MvnI CGCG 3 cut(s) 68, 150, 426
MwoI GCNNNNNNNGC 4 cut(s) 56, 65, 220, 430
NcoI CCATGG 2 cut(s) 51, 321
NdeII GATC 1 cut(s) 129
NlaIII CATG 2 cut(s) 55, 325
NruI TCGCGA 1 cut(s) 150
OliI CACNNNNGTG 1 cut(s) 88
PaqCI CACCTGC 1 cut(s) 296
PkrI GCNGC 5 cut(s) 58, 394, 448, 451, 467
PspPI GGNCC 1 cut(s) 351
RruI TCGCGA 1 cut(s) 150
RseI CAYNNNNRTG 2 cut(s) 88, 320
SaqAI TTAA 2 cut(s) 335, 347
SatI GCNGC 5 cut(s) 57, 393, 447, 450, 466
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 1 cut(s) 351
SduI GDGCHC 1 cut(s) 387
SetI ASST 4 cut(s) 119, 216, 290, 353
SfaNI GCATC 1 cut(s) 371
SinI GGWCC 1 cut(s) 351
SmiMI CAYNNNNRTG 2 cut(s) 88, 320
Sse9I AATT 2 cut(s) 43, 255
SsiI CCGC 7 cut(s) 56, 59, 68, 426, 444, 450, 465
StyI CCWWGG 3 cut(s) 51, 272, 321
TaaI ACNGT 2 cut(s) 87, 441
TaqI TCGA 2 cut(s) 24, 156
TasI AATT 2 cut(s) 43, 255
TauI GCSGC 3 cut(s) 59, 452, 468
Tru1I TTAA 2 cut(s) 335, 347
Tru9I TTAA 2 cut(s) 335, 347
TscAI CASTG 1 cut(s) 230
TseI GCWGC 2 cut(s) 392, 446
TspRI CASTG 1 cut(s) 230
VneI GTGCAC 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 351
XapI RAATTY 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.