MD17G1095600.v1.1

Transmembrane protein 97-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0024307
Physical Location & Seq
Reverse (-)
8093267 .. 8094647
1381 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1095600.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGGTGCTTTTGTGAAGATAATCGACGCCATACTCTTCGTAATGTTCGCCATAGCGGCGGCGAACGCGGTGCTAATAGACGTGCAGTTGTGTCTACCTCAGAACCTCTTCACAAGCTCGCTGGTGGAGCTCAAGAACTGGTATGTCAGGGAGTTCGACGATTATCTTGGTGAAGAGAAGCCTCATTTCTTTGTTGGTCTCGTTTGGGTTGAGCTCATACTGCAGTGGCCCCTTCTCGTTGCCAACTTGTATGGGATTTTGGCAGCTAAGCCTTGGTACAGAACCACTTGCTTGCTCTATGGCGCTTCTTTCTTCACCACCATGGGCGCTATTTTACCAGAGCTGTTATGGTCCGGCAAGGCATCTAATACAATGTTGATGTTGTACTACCCTGCTGTGGGCTTTGCCATCCTGGCCATACTGCGCGGGTTGCTCCCTCCATCTACCGCCAATACTTCAACCAGTGGCAAAAGAACAACAAAAAGCACCGGCCAACCAAAGTATCGGAAAGAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2781

Protein Analysis

173

Amino Acids

19.19

Weight (kDa)

8.87

Isoelectric Point (pI)

27.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 41 - 120 2.3e-11 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024307)

Species Orthologous Gene IDs
malus_domestica MD09G1107500.v1.1 MD17G1095600.v1.1
prunus_persica Prupe.3G218200_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 94
AccII CGCG 2 cut(s) 68, 426
AciI CCGC 5 cut(s) 56, 59, 68, 426, 447
AcoI YGGCCR 2 cut(s) 414, 490
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 2 cut(s) 278, 386
AfiI CCNNNNNNNGG 2 cut(s) 397, 398
AgsI TTSAA 1 cut(s) 459
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 1 cut(s) 411
AluBI AGCT 5 cut(s) 117, 130, 214, 266, 343
AluI AGCT 5 cut(s) 117, 130, 214, 266, 343
Alw21I GWGCWC 2 cut(s) 132, 216
Alw26I GTCTC 1 cut(s) 203
AoxI GGCC 3 cut(s) 227, 414, 490
ApeKI GCWGC 1 cut(s) 263
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 3 cut(s) 305, 329, 426
AspS9I GGNCC 2 cut(s) 228, 351
AsuHPI GGTGA 2 cut(s) 182, 307
AvaII GGWCC 1 cut(s) 351
BaeI ACNNNNGTAYC 2 cut(s) 485, 518
BalI TGGCCA 1 cut(s) 416
BanII GRGCYC 2 cut(s) 132, 216
Bbv12I GWGCWC 2 cut(s) 132, 216
BbvI GCAGC 1 cut(s) 275
BccI CCATC 2 cut(s) 416, 448
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BciT130I CCWGG 1 cut(s) 413
BcoDI GTCTC 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 221
BfoI RGCGCY 2 cut(s) 306, 330
BglI GCCNNNNNGGC 2 cut(s) 56, 413
BisI GCNGC 3 cut(s) 57, 60, 264
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 3 cut(s) 58, 61, 265
Bme1390I CCNGG 1 cut(s) 413
Bme18I GGWCC 1 cut(s) 351
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 228, 351
BmiI GGNNCC 1 cut(s) 230
BmrFI CCNGG 1 cut(s) 413
BmsI GCATC 1 cut(s) 371
Bpu1102I GCTNAGC 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 116
BsaHI GRCGYC 1 cut(s) 27
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 2 cut(s) 272, 321
Bsc4I CCNNNNNNNGG 2 cut(s) 397, 398
Bse118I RCCGGY 1 cut(s) 488
Bse1I ACTGG 2 cut(s) 143, 462
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 2 cut(s) 272, 321
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 397, 398
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 2 cut(s) 143, 462
BseXI GCAGC 1 cut(s) 275
BsgI GTGCAG 1 cut(s) 104
Bsh1236I CGCG 2 cut(s) 68, 426
BshFI GGCC 3 cut(s) 229, 416, 492
BsiHKAI GWGCWC 2 cut(s) 132, 216
BsiSI CCGG 2 cut(s) 354, 489
BslI CCNNNNNNNGG 2 cut(s) 397, 398
BsmAI GTCTC 1 cut(s) 203
BsnI GGCC 3 cut(s) 229, 416, 492
Bso31I GGTCTC 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 132, 216
Bsp1720I GCTNAGC 1 cut(s) 267
Bsp19I CCATGG 1 cut(s) 321
BspACI CCGC 5 cut(s) 56, 59, 68, 426, 447
BspANI GGCC 3 cut(s) 229, 416, 492
BspCNI CTCAG 1 cut(s) 112
BspFNI CGCG 2 cut(s) 68, 426
BspLI GGNNCC 1 cut(s) 230
BspMAI CTGCAG 1 cut(s) 225
BspTNI GGTCTC 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 488
BsrI ACTGG 2 cut(s) 143, 462
BssAI RCCGGY 1 cut(s) 488
BssECI CCNNGG 2 cut(s) 272, 321
BssNI GRCGYC 1 cut(s) 27
BssT1I CCWWGG 2 cut(s) 272, 321
Bst2UI CCWGG 1 cut(s) 413
Bst6I CTCTTC 3 cut(s) 41, 113, 168
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 119, 293
BstDEI CTNAG 3 cut(s) 99, 267, 516
BstDSI CCRYGG 1 cut(s) 321
BstF5I GGATG 1 cut(s) 408
BstFNI CGCG 2 cut(s) 68, 426
BstH2I RGCGCY 2 cut(s) 306, 330
BstHHI GCGC 3 cut(s) 305, 329, 426
BstMAI GTCTC 1 cut(s) 203
BstMWI GCNNNNNNNGC 6 cut(s) 56, 65, 127, 220, 413, 430
BstNI CCWGG 1 cut(s) 413
BstSCI CCNGG 1 cut(s) 411
BstSFI CTRYAG 1 cut(s) 221
BstUI CGCG 2 cut(s) 68, 426
BstV1I GCAGC 1 cut(s) 275
BsuRI GGCC 3 cut(s) 229, 416, 492
BtgI CCRYGG 1 cut(s) 321
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 408
BtsI GCAGTG 1 cut(s) 230
BtsIMutI CAGTG 2 cut(s) 230, 469
Cac8I GCNNGC 2 cut(s) 119, 293
CfoI GCGC 3 cut(s) 305, 329, 426
Cfr10I RCCGGY 1 cut(s) 488
Cfr13I GGNCC 2 cut(s) 228, 351
CseI GACGC 1 cut(s) 35
Csp6I GTAC 2 cut(s) 277, 385
CviAII CATG 1 cut(s) 322
CviQI GTAC 2 cut(s) 277, 385
DdeI CTNAG 3 cut(s) 99, 267, 516
EaeI YGGCCR 2 cut(s) 414, 490
Eam1104I CTCTTC 3 cut(s) 41, 113, 168
EarI CTCTTC 3 cut(s) 41, 113, 168
Ecl136II GAGCTC 2 cut(s) 130, 214
Eco130I CCWWGG 2 cut(s) 272, 321
Eco24I GRGCYC 2 cut(s) 132, 216
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 351
Eco53kI GAGCTC 2 cut(s) 130, 214
EcoICRI GAGCTC 2 cut(s) 130, 214
EcoRII CCWGG 1 cut(s) 411
EcoT14I CCWWGG 2 cut(s) 272, 321
EcoT38I GRGCYC 2 cut(s) 132, 216
ErhI CCWWGG 2 cut(s) 272, 321
FaeI CATG 1 cut(s) 325
FaiI YATR 9 cut(s) 32, 53, 144, 218, 252, 300, 323, 349, 419
FatI CATG 1 cut(s) 321
FauI CCCGC 1 cut(s) 419
FblI GTMKAC 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 57, 60, 264
FokI GGATG 1 cut(s) 395
FriOI GRGCYC 2 cut(s) 132, 216
Fsp4HI GCNGC 3 cut(s) 57, 60, 264
GlaI GCGC 3 cut(s) 304, 328, 425
GluI GCNGC 3 cut(s) 57, 60, 264
HaeII RGCGCY 2 cut(s) 306, 330
HaeIII GGCC 3 cut(s) 229, 416, 492
HapII CCGG 2 cut(s) 354, 489
HgaI GACGC 1 cut(s) 35
HhaI GCGC 3 cut(s) 305, 329, 426
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 1 cut(s) 325
Hin6I GCGC 3 cut(s) 303, 327, 424
HinP1I GCGC 3 cut(s) 303, 327, 424
HpaII CCGG 2 cut(s) 354, 489
HphI GGTGA 2 cut(s) 182, 307
Hpy166II GTNNAC 1 cut(s) 95
Hpy188I TCNGA 2 cut(s) 102, 507
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 1 cut(s) 95
Hpy99I CGWCG 2 cut(s) 29, 161
HpyAV CCTTC 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 2 cut(s) 85, 223
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 65, 127, 220, 413, 430
HpyF3I CTNAG 3 cut(s) 99, 267, 516
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 1 cut(s) 325
HspAI GCGC 3 cut(s) 303, 327, 424
LmnI GCTCC 2 cut(s) 127, 438
Lsp1109I GCAGC 1 cut(s) 275
LweI GCATC 1 cut(s) 371
MaeII ACGT 1 cut(s) 81
MboII GAAGA 5 cut(s) 28, 28, 100, 185, 304
MhlI GDGCHC 2 cut(s) 132, 216
MlsI TGGCCA 1 cut(s) 416
MluNI TGGCCA 1 cut(s) 416
MnlI CCTC 4 cut(s) 108, 116, 192, 447
Mox20I TGGCCA 1 cut(s) 416
MroXI GAANNNNTTC 1 cut(s) 107
MscI TGGCCA 1 cut(s) 416
MslI CAYNNNNRTG 1 cut(s) 320
Msp20I TGGCCA 1 cut(s) 416
MspI CCGG 2 cut(s) 354, 489
MspR9I CCNGG 1 cut(s) 413
MvaI CCWGG 1 cut(s) 413
MvnI CGCG 2 cut(s) 68, 426
MwoI GCNNNNNNNGC 6 cut(s) 56, 65, 127, 220, 413, 430
NcoI CCATGG 1 cut(s) 321
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 107
PkrI GCNGC 3 cut(s) 58, 61, 265
Psp124BI GAGCTC 2 cut(s) 132, 216
Psp6I CCWGG 1 cut(s) 411
PspGI CCWGG 1 cut(s) 411
PspN4I GGNNCC 1 cut(s) 230
PspPI GGNCC 2 cut(s) 228, 351
PstI CTGCAG 1 cut(s) 225
RsaI GTAC 2 cut(s) 278, 386
RsaNI GTAC 2 cut(s) 277, 385
RseI CAYNNNNRTG 1 cut(s) 320
SacI GAGCTC 2 cut(s) 132, 216
SatI GCNGC 3 cut(s) 57, 60, 264
Sau96I GGNCC 2 cut(s) 228, 351
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 2 cut(s) 132, 216
SetI ASST 8 cut(s) 84, 100, 108, 119, 132, 216, 268, 345
SfaNI GCATC 1 cut(s) 371
SfcI CTRYAG 1 cut(s) 221
SinI GGWCC 1 cut(s) 351
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SsiI CCGC 5 cut(s) 56, 59, 68, 426, 447
SstI GAGCTC 2 cut(s) 132, 216
StyD4I CCNGG 1 cut(s) 411
StyI CCWWGG 2 cut(s) 272, 321
TaiI ACGT 1 cut(s) 84
TaqI TCGA 2 cut(s) 24, 156
TatI WGTACW 1 cut(s) 384
TauI GCSGC 2 cut(s) 59, 62
TscAI CASTG 2 cut(s) 230, 469
TseI GCWGC 1 cut(s) 263
TspRI CASTG 2 cut(s) 230, 469
VpaK11BI GGWCC 1 cut(s) 351
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XmiI GTMKAC 1 cut(s) 94
XmnI GAANNNNTTC 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.