Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0024308

Physical Location & Seq

Genomic Coordinates Forward (+)

12238515 .. 12239564

Gene Structure 1050 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1154200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1050 bp

ATGGCGAATGTGTACAAACCGAGGAACATCCTCATTACTGGAGCTGCCGGCTTCATTGGTTCCCATGTTGTCAACCAACTCATGCGGAACTATCCTGAGTACAAGATTTTGGTCCTTGACAAGCTTGATTACTGTTCAAATTTGAAAAACCTTCACCCTTCACATTCGTCACCCAACCTTAAGTTTATCAAGGGAGACATCTGCAGTGCTGACTTTGTCAATTCAATTCTTCTCAACGAGTCCATTGATACAATAATGCACTTTGCAGCCCAGACCCATGTCGACAACTCTTTCGGTAACAGCTTTGAGTTCACTAAAAATAACGTCTACGGTACACAGGTTCTCTTAGAAGCATGCAAAGTCACTGGTCAAATCAAAAGGTTCATTCATGTAAGCACAGACGAAGTGTATGGGGAGACAGATGAGAATGCTGTGGTGGGAAATCATGAGGCTTCTCAGCTTCTTCCGACAAAGCCCTACTCTGCAACTAAAGCTGGATCGGAGATGCTTGTCATGGCGTATGGGCGTTCATATGGATTGCCGGTGATAACGACCAGAGGAAATAATGTTTACGGCCCCAATCAGTTTCCTGAAAAGATGATTCCGAAATTCATTCTATTGGCTATGCAAGGGAAGCGTCTTCCGATTTATGGAAATGGATCCAATTTCAGAAGTTATCTCTATTGTGAGGATGTCGCAGAGGCATTTGAAGTCATTCTTCATAGGGGTGAGGTAGGCTGTGTCTACAACATCGGGACAAAGAAAGAGAGGAAATTGATTGATGTGGCTAGGGAAATTTGCCAACACTTCTCTTTGAACCCAGATGCACATATTAAGTTTGTGCAGGATAGGCCTTTTCATGATAAGAGATATTTCATTGATTACAAGAAGCTGAAAGCCTTGGGATGGCCCCAACAGACGTCGTGGGAGGATGGGTTGAAGAAGACATTGGACTGGTACGTGAAGAATCCCTACTGGTGGGGGGATGTTTCTGGAACACTACTTGCTCGCCCGAGAAACTGCAAAGTTTCTTCAAATTTTTCTTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

350

Amino Acids

39.65

Weight (kDa)

8.77

Isoelectric Point (pI)

23.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	9 - 318	7.2e-16	RmlD substrate binding domain
Epimerase	PF01370	10 - 252	6.2e-58	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	10 - 281	8.7e-21	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	11 - 316	5.4e-71	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	11 - 235	3.9e-27	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	12 - 196	2.4e-20	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0024308)

Species	Orthologous Gene IDs
malus_domestica	MD09G1154200.v1.1 MD09G1154400.v1.1
pyrus_communis	pycom09g07330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	923
AccI	GTMKAC	3 cut(s)	282, 327, 744
AciI	CCGC	1 cut(s)	85
AclWI	GGATC	3 cut(s)	505, 654, 667
AcsI	RAATTY	4 cut(s)	139, 608, 795, 1036
AcyI	GRCGYC	1 cut(s)	920
AfaI	GTAC	4 cut(s)	14, 101, 334, 959
AfiI	CCNNNNNNNGG	3 cut(s)	650, 906, 978
AflII	CTTAAG	1 cut(s)	179
AgsI	TTSAA	7 cut(s)	138, 145, 225, 710, 817, 940, 1035
AjuI	GAANNNNNNNTTGG	2 cut(s)	932, 964
AluBI	AGCT	6 cut(s)	44, 124, 303, 460, 494, 892
AluI	AGCT	6 cut(s)	44, 124, 303, 460, 494, 892
Alw26I	GTCTC	2 cut(s)	189, 410
AlwI	GGATC	3 cut(s)	505, 654, 667
Ama87I	CYCGRG	1 cut(s)	1012
AoxI	GGCC	3 cut(s)	574, 851, 908
ApeKI	GCWGC	2 cut(s)	44, 266
ApoI	RAATTY	4 cut(s)	139, 608, 795, 1036
ArsI	GACNNNNNNTTYG	2 cut(s)	275, 307
Asp700I	GAANNNNTTC	1 cut(s)	714
AspS9I	GGNCC	3 cut(s)	112, 575, 909
AsuHPI	GGTGA	4 cut(s)	146, 162, 556, 740
AvaI	CYCGRG	1 cut(s)	1012
AvaII	GGWCC	1 cut(s)	112
BaeI	ACNNNNGTAYC	2 cut(s)	324, 357
BamHI	GGATCC	1 cut(s)	659
BarI	GAAGNNNNNNTAC	2 cut(s)	956, 988
BbsI	GAAGAC	2 cut(s)	632, 950
BbvI	GCAGC	2 cut(s)	31, 278
BccI	CCATC	2 cut(s)	900, 926
BceAI	ACGGC	1 cut(s)	589
BcoDI	GTCTC	2 cut(s)	189, 410
BfaI	CTAG	1 cut(s)	789
BfmI	CTRYAG	1 cut(s)	202
BfrI	CTTAAG	1 cut(s)	179
BisI	GCNGC	2 cut(s)	45, 267
BlsI	GCNGC	2 cut(s)	46, 268
Bme18I	GGWCC	1 cut(s)	112
BmeT110I	CYCGRG	1 cut(s)	1012
BmgT120I	GGNCC	3 cut(s)	112, 575, 909
BmiI	GGNNCC	4 cut(s)	61, 577, 661, 911
BmsI	GCATC	2 cut(s)	495, 814
BoxI	GACNNNNGTC	1 cut(s)	278
BpiI	GAAGAC	2 cut(s)	632, 950
BpmI	CTGGAG	1 cut(s)	60
BsaAI	YACGTR	1 cut(s)	961
BsaHI	GRCGYC	1 cut(s)	920
BsaJI	CCNNGG	2 cut(s)	20, 900
BsaXI	ACNNNNNCTCC	2 cut(s)	920, 950
Bsc4I	CCNNNNNNNGG	3 cut(s)	650, 906, 978
Bse118I	RCCGGY	2 cut(s)	47, 541
Bse1I	ACTGG	4 cut(s)	43, 370, 959, 980
BseDI	CCNNGG	2 cut(s)	20, 900
BseGI	GGATG	5 cut(s)	27, 697, 911, 937, 991
BseLI	CCNNNNNNNGG	3 cut(s)	650, 906, 978
BseMII	CTCAG	2 cut(s)	87, 470
BseNI	ACTGG	4 cut(s)	43, 370, 959, 980
BseXI	GCAGC	2 cut(s)	31, 278
BsgI	GTGCAG	1 cut(s)	863
BshFI	GGCC	3 cut(s)	576, 853, 910
BsiHKCI	CYCGRG	1 cut(s)	1012
BsiSI	CCGG	2 cut(s)	48, 542
BslFI	GGGAC	1 cut(s)	769
BslI	CCNNNNNNNGG	3 cut(s)	650, 906, 978
BsmAI	GTCTC	2 cut(s)	189, 410
BsmFI	GGGAC	1 cut(s)	769
BsmI	GAATGC	1 cut(s)	433
BsnI	GGCC	3 cut(s)	576, 853, 910
BsoBI	CYCGRG	1 cut(s)	1012
Bsp1407I	TGTACA	1 cut(s)	12
Bsp143I	GATC	2 cut(s)	497, 659
BspACI	CCGC	1 cut(s)	85
BspANI	GGCC	3 cut(s)	576, 853, 910
BspCNI	CTCAG	2 cut(s)	88, 469
BspHI	TCATGA	2 cut(s)	445, 859
BspLI	GGNNCC	4 cut(s)	61, 577, 661, 911
BspMAI	CTGCAG	1 cut(s)	206
BspPI	GGATC	3 cut(s)	505, 654, 667
BspTI	CTTAAG	1 cut(s)	179
BsrFI	RCCGGY	2 cut(s)	47, 541
BsrGI	TGTACA	1 cut(s)	12
BsrI	ACTGG	4 cut(s)	43, 370, 959, 980
BssAI	RCCGGY	2 cut(s)	47, 541
BssECI	CCNNGG	2 cut(s)	20, 900
BssMI	GATC	2 cut(s)	497, 659
BssNI	GRCGYC	1 cut(s)	920
BssT1I	CCWWGG	1 cut(s)	900
Bst4CI	ACNGT	2 cut(s)	134, 332
BstACI	GRCGYC	1 cut(s)	920
BstAFI	CTTAAG	1 cut(s)	179
BstAUI	TGTACA	1 cut(s)	12
BstBAI	YACGTR	1 cut(s)	961
BstC8I	GCNNGC	3 cut(s)	49, 355, 1009
BstDEI	CTNAG	3 cut(s)	96, 346, 456
BstF5I	GGATG	5 cut(s)	27, 697, 911, 937, 991
BstKTI	GATC	2 cut(s)	500, 662
BstMAI	GTCTC	2 cut(s)	189, 410
BstMBI	GATC	2 cut(s)	497, 659
BstMWI	GCNNNNNNNGC	3 cut(s)	491, 634, 850
BstNSI	RCATGY	1 cut(s)	357
BstPAI	GACNNNNGTC	1 cut(s)	278
BstSFI	CTRYAG	1 cut(s)	202
BstV1I	GCAGC	2 cut(s)	31, 278
BstV2I	GAAGAC	2 cut(s)	632, 950
BstX2I	RGATCY	1 cut(s)	659
BstYI	RGATCY	1 cut(s)	659
BsuRI	GGCC	3 cut(s)	576, 853, 910
BtsCI	GGATG	5 cut(s)	27, 697, 911, 937, 991
BtsI	GCAGTG	1 cut(s)	211
BtsIMutI	CAGTG	2 cut(s)	211, 363
Cac8I	GCNNGC	3 cut(s)	49, 355, 1009
CciI	TCATGA	2 cut(s)	445, 859
Cfr10I	RCCGGY	2 cut(s)	47, 541
Cfr13I	GGNCC	3 cut(s)	112, 575, 909
CseI	GACGC	1 cut(s)	626
Csp6I	GTAC	4 cut(s)	13, 100, 333, 958
CviAII	CATG	8 cut(s)	65, 82, 278, 354, 389, 446, 514, 860
CviQI	GTAC	4 cut(s)	13, 100, 333, 958
DdeI	CTNAG	3 cut(s)	96, 346, 456
DpnI	GATC	2 cut(s)	499, 661
DpnII	GATC	2 cut(s)	497, 659
Eco130I	CCWWGG	1 cut(s)	900
Eco147I	AGGCCT	1 cut(s)	853
Eco47I	GGWCC	1 cut(s)	112
Eco88I	CYCGRG	1 cut(s)	1012
EcoT14I	CCWWGG	1 cut(s)	900
ErhI	CCWWGG	1 cut(s)	900
FaeI	CATG	8 cut(s)	68, 85, 281, 357, 392, 449, 517, 863
FalI	AAGNNNNNCTT	2 cut(s)	702, 734
FaqI	GGGAC	1 cut(s)	769
FatI	CATG	8 cut(s)	64, 81, 277, 353, 388, 445, 513, 859
FauNDI	CATATG	1 cut(s)	532
FblI	GTMKAC	3 cut(s)	282, 327, 744
Fnu4HI	GCNGC	2 cut(s)	45, 267
FokI	GGATG	5 cut(s)	14, 704, 918, 944, 998
Fsp4HI	GCNGC	2 cut(s)	45, 267
FspBI	CTAG	1 cut(s)	789
GluI	GCNGC	2 cut(s)	45, 267
GsuI	CTGGAG	1 cut(s)	60
HaeIII	GGCC	3 cut(s)	576, 853, 910
HapII	CCGG	2 cut(s)	48, 542
HgaI	GACGC	1 cut(s)	626
Hin1I	GRCGYC	1 cut(s)	920
Hin1II	CATG	8 cut(s)	68, 85, 281, 357, 392, 449, 517, 863
HincII	GTYRAC	2 cut(s)	73, 283
HindII	GTYRAC	2 cut(s)	73, 283
HindIII	AAGCTT	1 cut(s)	122
HinfI	GANTC	3 cut(s)	239, 601, 967
HpaII	CCGG	2 cut(s)	48, 542
HphI	GGTGA	4 cut(s)	146, 162, 556, 740
Hpy166II	GTNNAC	8 cut(s)	13, 73, 283, 312, 328, 335, 571, 745
Hpy188I	TCNGA	5 cut(s)	468, 502, 606, 645, 671
Hpy188III	TCNNGA	6 cut(s)	95, 446, 590, 754, 860, 993
Hpy8I	GTNNAC	8 cut(s)	13, 73, 283, 312, 328, 335, 571, 745
Hpy99I	CGWCG	1 cut(s)	925
HpyAV	CCTTC	2 cut(s)	161, 168
HpyCH4III	ACNGT	2 cut(s)	134, 332
HpyCH4IV	ACGT	3 cut(s)	324, 920, 960
HpyCH4V	TGCA	9 cut(s)	204, 259, 266, 357, 485, 628, 827, 844, 1023
HpyF10VI	GCNNNNNNNGC	3 cut(s)	491, 634, 850
HpyF3I	CTNAG	3 cut(s)	96, 346, 456
HpySE526I	ACGT	3 cut(s)	324, 920, 960
Hsp92I	GRCGYC	1 cut(s)	920
Hsp92II	CATG	8 cut(s)	68, 85, 281, 357, 392, 449, 517, 863
KroI	GCCGGC	1 cut(s)	47
KroNI	GCCGGC	1 cut(s)	49
Kzo9I	GATC	2 cut(s)	497, 659
LmnI	GCTCC	1 cut(s)	41
Lsp1109I	GCAGC	2 cut(s)	31, 278
LweI	GCATC	2 cut(s)	495, 814
MaeI	CTAG	1 cut(s)	789
MaeII	ACGT	3 cut(s)	324, 920, 960
MaeIII	GTNAC	3 cut(s)	168, 296, 361
MalI	GATC	2 cut(s)	499, 661
MboI	GATC	2 cut(s)	497, 659
MboII	GAAGA	8 cut(s)	221, 455, 632, 710, 952, 955, 976, 1023
MflI	RGATCY	1 cut(s)	659
MluCI	AATT	8 cut(s)	139, 220, 225, 608, 664, 773, 795, 1036
MlyI	GAGTC	1 cut(s)	248
MmeI	TCCRAC	1 cut(s)	491
MnlI	CCTC	9 cut(s)	15, 41, 442, 551, 682, 694, 724, 762, 922
MroNI	GCCGGC	1 cut(s)	47
MroXI	GAANNNNTTC	1 cut(s)	714
MseI	TTAA	3 cut(s)	180, 834, 1048
MslI	CAYNNNNRTG	1 cut(s)	726
MspCI	CTTAAG	1 cut(s)	179
MspI	CCGG	2 cut(s)	48, 542
Mva1269I	GAATGC	1 cut(s)	433
MwoI	GCNNNNNNNGC	3 cut(s)	491, 634, 850
NaeI	GCCGGC	1 cut(s)	49
NdeI	CATATG	1 cut(s)	532
NdeII	GATC	2 cut(s)	497, 659
NgoMIV	GCCGGC	1 cut(s)	47
NlaIII	CATG	8 cut(s)	68, 85, 281, 357, 392, 449, 517, 863
NlaIV	GGNNCC	4 cut(s)	61, 577, 661, 911
NmuCI	GTSAC	2 cut(s)	168, 361
NspI	RCATGY	1 cut(s)	357
PaeI	GCATGC	1 cut(s)	357
PagI	TCATGA	2 cut(s)	445, 859
PceI	AGGCCT	1 cut(s)	853
PctI	GAATGC	1 cut(s)	433
PdiI	GCCGGC	1 cut(s)	49
PdmI	GAANNNNTTC	1 cut(s)	714
PfeI	GAWTC	2 cut(s)	601, 967
PflFI	GACNNNGTC	1 cut(s)	215
PkrI	GCNGC	2 cut(s)	46, 268
PleI	GAGTC	1 cut(s)	247
PpsI	GAGTC	1 cut(s)	247
Ppu21I	YACGTR	1 cut(s)	961
PshAI	GACNNNNGTC	1 cut(s)	278
PspN4I	GGNNCC	4 cut(s)	61, 577, 661, 911
PspPI	GGNCC	3 cut(s)	112, 575, 909
PstI	CTGCAG	1 cut(s)	206
PsuI	RGATCY	1 cut(s)	659
PsyI	GACNNNGTC	1 cut(s)	215
RsaI	GTAC	4 cut(s)	14, 101, 334, 959
RsaNI	GTAC	4 cut(s)	13, 100, 333, 958
RseI	CAYNNNNRTG	1 cut(s)	726
SalI	GTCGAC	1 cut(s)	281
SaqAI	TTAA	3 cut(s)	180, 834, 1048
SatI	GCNGC	2 cut(s)	45, 267
Sau3AI	GATC	2 cut(s)	497, 659
Sau96I	GGNCC	3 cut(s)	112, 575, 909
SchI	GAGTC	1 cut(s)	248
SfaNI	GCATC	2 cut(s)	495, 814
SfcI	CTRYAG	1 cut(s)	202
SinI	GGWCC	1 cut(s)	112
SmiMI	CAYNNNNRTG	1 cut(s)	726
SmlI	CTYRAG	1 cut(s)	179
SmoI	CTYRAG	1 cut(s)	179
SphI	GCATGC	1 cut(s)	357
Sse9I	AATT	8 cut(s)	139, 220, 225, 608, 664, 773, 795, 1036
SseBI	AGGCCT	1 cut(s)	853
SsiI	CCGC	1 cut(s)	85
SspMI	CTAG	1 cut(s)	789
StuI	AGGCCT	1 cut(s)	853
StyI	CCWWGG	1 cut(s)	900
TaaI	ACNGT	2 cut(s)	134, 332
TaiI	ACGT	3 cut(s)	327, 923, 963
TaqI	TCGA	1 cut(s)	282
TasI	AATT	8 cut(s)	139, 220, 225, 608, 664, 773, 795, 1036
TatI	WGTACW	2 cut(s)	12, 99
TfiI	GAWTC	2 cut(s)	601, 967
Tru1I	TTAA	3 cut(s)	180, 834, 1048
Tru9I	TTAA	3 cut(s)	180, 834, 1048
TscAI	CASTG	2 cut(s)	211, 370
TseFI	GTSAC	2 cut(s)	168, 361
TseI	GCWGC	2 cut(s)	44, 266
Tsp45I	GTSAC	2 cut(s)	168, 361
TspDTI	ATGAA	8 cut(s)	43, 373, 377, 519, 601, 710, 848, 865
TspRI	CASTG	2 cut(s)	211, 370
Tth111I	GACNNNGTC	1 cut(s)	215
Vha464I	CTTAAG	1 cut(s)	179
VpaK11BI	GGWCC	1 cut(s)	112
XapI	RAATTY	4 cut(s)	139, 608, 795, 1036
XceI	RCATGY	1 cut(s)	357
XmiI	GTMKAC	3 cut(s)	282, 327, 744
XmnI	GAANNNNTTC	1 cut(s)	714
XspI	CTAG	1 cut(s)	789
ZraI	GACGTC	1 cut(s)	921

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.