MD09G1218100.v1.1
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0027305
Physical Location & Seq
Reverse (-)
21965460 .. 21965819
360 bp
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UTR
Exon/CDS
Intron
MD09G1218100.v1.1.491

Sequence Viewer

Length: 360 bp
ATGATCAAGGACGCTGAGCTGCATGCTCAAAGGGATCAGGAGCGGAAAGTTCCCGTCGATGTCAAAAACAGCGCGGACACCACCATCTACATCATTGAGAAGAGCTTGAACGAGTACTGCGACAAGGTTCCAAGCGAAATTGCTCAAGAAATCGAGGATGCAGTTGGTGACTTGAGGACGGCAATTGGAGGGGACAATGCTGATGAGATTAAGTCGAAGCTTGACGTTGCAAACAAAGCCGTGTCAAAGATCGGTGAGCACATGTCGAAGGGTTCTGGAGGCGGTGACTCTTCGTCTGGAGGATCACAGGACGGAGGAGACGAGGCTCCTGAGTCAGAGCACCAAGAGATGAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03018 ko04212 ko05152 map03018 map04212 map05152
Pfam Domains
Protein Families
HSP70

Protein Analysis

120

Amino Acids

12.76

Weight (kDa)

4.62

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 1 - 89 1.5e-12 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0027305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32208
malus_domestica MD09G1218100.v1.1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 43
AccII CGCG 1 cut(s) 74
AciI CCGC 3 cut(s) 43, 74, 282
AclWI GGATC 2 cut(s) 42, 310
AfaI GTAC 1 cut(s) 116
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 1 cut(s) 109
AhdI GACNNNNNGTC 1 cut(s) 292
AluBI AGCT 3 cut(s) 19, 105, 220
AluI AGCT 3 cut(s) 19, 105, 220
Alw21I GWGCWC 2 cut(s) 261, 342
Alw26I GTCTC 1 cut(s) 312
AlwI GGATC 2 cut(s) 42, 310
ApeKI GCWGC 1 cut(s) 19
AspLEI GCGC 1 cut(s) 74
AsuHPI GGTGA 3 cut(s) 179, 266, 296
Bbv12I GWGCWC 2 cut(s) 261, 342
BbvI GCAGC 1 cut(s) 6
BccI CCATC 1 cut(s) 92
BceAI ACGGC 2 cut(s) 195, 224
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 312
BisI GCNGC 1 cut(s) 20
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 21
BmcAI AGTACT 1 cut(s) 116
BmeRI GACNNNNNGTC 1 cut(s) 292
BmiI GGNNCC 2 cut(s) 129, 327
BmsI GCATC 1 cut(s) 148
BpmI CTGGAG 2 cut(s) 297, 318
Bpu1102I GCTNAGC 1 cut(s) 15
BpuEI CTTGAG 2 cut(s) 129, 193
BseGI GGATG 1 cut(s) 163
BseMII CTCAG 2 cut(s) 6, 321
BseRI GAGGAG 1 cut(s) 330
BseXI GCAGC 1 cut(s) 6
Bsh1236I CGCG 1 cut(s) 74
BsiHKAI GWGCWC 2 cut(s) 261, 342
BslFI GGGAC 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 312
BsmBI CGTCTC 1 cut(s) 312
BsmFI GGGAC 1 cut(s) 206
Bsp1286I GDGCHC 2 cut(s) 261, 342
Bsp143I GATC 4 cut(s) 3, 34, 249, 302
Bsp1720I GCTNAGC 1 cut(s) 15
BspACI CCGC 3 cut(s) 43, 74, 282
BspCNI CTCAG 2 cut(s) 7, 322
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 2 cut(s) 129, 327
BspPI GGATC 2 cut(s) 42, 310
BspQI GCTCTTC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 43
BssMI GATC 4 cut(s) 3, 34, 249, 302
Bst6I CTCTTC 2 cut(s) 95, 295
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 2 cut(s) 15, 330
BstF5I GGATG 1 cut(s) 163
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 4 cut(s) 6, 37, 252, 305
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 4 cut(s) 3, 34, 249, 302
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNSI RCATGY 2 cut(s) 26, 265
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 6
BtsCI GGATG 1 cut(s) 163
Cac8I GCNNGC 1 cut(s) 24
CfoI GCGC 1 cut(s) 74
CseI GACGC 1 cut(s) 20
Csp6I GTAC 1 cut(s) 115
CviAII CATG 2 cut(s) 23, 262
CviJI RGCY 5 cut(s) 19, 105, 220, 239, 326
CviKI_1 RGCY 5 cut(s) 19, 105, 220, 239, 326
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 2 cut(s) 15, 330
DpnI GATC 4 cut(s) 5, 36, 251, 304
DpnII GATC 4 cut(s) 3, 34, 249, 302
DriI GACNNNNNGTC 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 95, 295
Eam1105I GACNNNNNGTC 1 cut(s) 292
EarI CTCTTC 2 cut(s) 95, 295
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 2 cut(s) 26, 265
FaiI YATR 2 cut(s) 24, 263
FaqI GGGAC 1 cut(s) 206
FatI CATG 2 cut(s) 22, 261
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 20
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 1 cut(s) 20
GlaI GCGC 1 cut(s) 73
GluI GCNGC 1 cut(s) 20
GsuI CTGGAG 2 cut(s) 297, 318
HgaI GACGC 1 cut(s) 20
HhaI GCGC 1 cut(s) 74
Hin1II CATG 2 cut(s) 26, 265
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 2 cut(s) 287, 332
HphI GGTGA 3 cut(s) 179, 266, 296
Hpy188I TCNGA 1 cut(s) 337
Hpy188III TCNNGA 5 cut(s) 38, 146, 276, 297, 329
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 1 cut(s) 262
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 22, 161, 230
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 2 cut(s) 15, 330
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 2 cut(s) 26, 265
HspAI GCGC 1 cut(s) 72
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 34, 249, 302
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 2 cut(s) 40, 331
LpnPI CCDG 5 cut(s) 23, 261, 282, 293, 342
Lsp1109I GCAGC 1 cut(s) 6
LweI GCATC 1 cut(s) 148
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 2 cut(s) 167, 284
MalI GATC 4 cut(s) 5, 36, 251, 304
MbiI CCGCTC 1 cut(s) 43
MboI GATC 4 cut(s) 3, 34, 249, 302
MboII GAAGA 2 cut(s) 112, 282
MfeI CAATTG 1 cut(s) 183
MhlI GDGCHC 2 cut(s) 261, 342
MluCI AATT 2 cut(s) 138, 183
MlyI GAGTC 2 cut(s) 281, 341
MnlI CCTC 8 cut(s) 148, 168, 182, 272, 293, 308, 316, 345
MseI TTAA 1 cut(s) 210
MunI CAATTG 1 cut(s) 183
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 236
NdeII GATC 4 cut(s) 3, 34, 249, 302
NlaIII CATG 2 cut(s) 26, 265
NlaIV GGNNCC 2 cut(s) 129, 327
NmuCI GTSAC 2 cut(s) 167, 284
NspI RCATGY 2 cut(s) 26, 265
PaeI GCATGC 1 cut(s) 26
PciI ACATGT 1 cut(s) 261
PciSI GCTCTTC 1 cut(s) 95
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 318
PkrI GCNGC 1 cut(s) 21
PleI GAGTC 2 cut(s) 281, 340
PpsI GAGTC 2 cut(s) 281, 340
PscI ACATGT 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 129, 327
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 1 cut(s) 20
Sau3AI GATC 4 cut(s) 3, 34, 249, 302
ScaI AGTACT 1 cut(s) 116
SchI GAGTC 2 cut(s) 281, 341
SduI GDGCHC 2 cut(s) 261, 342
SetI ASST 5 cut(s) 21, 107, 129, 222, 228
SfaNI GCATC 1 cut(s) 148
SmlI CTYRAG 2 cut(s) 144, 172
SmoI CTYRAG 2 cut(s) 144, 172
SphI GCATGC 1 cut(s) 26
Sse9I AATT 2 cut(s) 138, 183
SsiI CCGC 3 cut(s) 43, 74, 282
TaiI ACGT 1 cut(s) 228
TaqI TCGA 4 cut(s) 57, 153, 215, 266
TasI AATT 2 cut(s) 138, 183
TatI WGTACW 1 cut(s) 114
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TseFI GTSAC 2 cut(s) 167, 284
TseI GCWGC 1 cut(s) 19
Tsp45I GTSAC 2 cut(s) 167, 284
TspGWI ACGGA 1 cut(s) 327
XceI RCATGY 2 cut(s) 26, 265
ZrmI AGTACT 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.