Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0020911

Physical Location & Seq

Genomic Coordinates Forward (+)

32644338 .. 32649861

Gene Structure 5524 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1254900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGCCCGCCACTCTCTTCCTCTCCCCTCTTTCCACTCTCCCAAACACCGCCCATCTTCTTCCCTCTCACGCGCAATTTTCCATCTCATCTTCCTTAAACCCAAAACCTCCACAAAACCCGACTCCCTCCAAAACCCCGCATTCCACGCCCCCATTGGAGTCCTTTTCGAGCTCCGACGCTTCCGTTAAGGCGCCTGCAGCTCCATGGATGAAGGGTCCGCTTCTTCTTCAACCCCACCAAGTCATTGACTTCTCAAAACCCAGAAATAGGAAGATCCCCGGCAATGCCAAACCCGAAAACTCCGATTCGGAGCTAGGAGGCAAGTTGGTCGGCATTAGAGGGGAGAGAGCAATCAAGCAAATTGTCCAGAGCATTGAGAGGCTTGGACCAAATGAGAATCCAGAAAAACCCCAGGAAGGTTTTGGGGAGTTTGGGATTTGGGATTGCTTGGAGGGGCTCGTACAAGAGGATAATTCGGTAGGAACCCACAGAGGTTTTGGGGAGTTTGGAATAGGGGATTGCTTGGAGGGACTTGGAAAGGCCAACGACTCGAGGATTTCCGGGAAGAAAATGCCGTGGGAGAGGGAGGAAAGGATGGTGTTTCCGAGAGTAAAGAGGGAAAGGGTGGCAAGTGCGGCAGAATTGAGCCTTGAGAAAGAGTTGCTTGAGAGGTTGAGAGGTGAGGCAGCAAAGTTGAGGAAATGGATCAAGGTTAAGAAGGCCGGGGTCACTCAAGCCGTTGTTGATGATGTTAAGTTCATTTGGAAGGGGAATGAGCTCGCCATGCTCAAATTCGACGTGCCACTCTGCCAGAATATGTATAGAGCTCAAGAGATTCTTGAGATGAAGACTGGAGGCATGGTTGTTTGGAGAAATAAAGACTCACTTGTTATTTATAGAGGGTGCAATTATCAATCAACTTCGAAATTTTTTCCAAAGAGGCACCCACGCTCTGCTGGCTGTCAAGAAACATCATCCTCAGATCTCATTCAGCTGGATTTAGAAAAAAGTAGTATTTATCAATCTGAAACTTTTGAAAGTGCTGTGGATGAAAAGTTGAACAAAAAGAACGACAAAGAGGATCCTACACAAATCTTTCTAGAAACCAATGTGAGTTGCCAACCAACAAGTAGGTCCTTGTATGAGAAGGAAGCAGATAGATTATTGGATGGCTTAGGACCTCGATTCATTGACTGGTGGATGCATAAGCCTTTGCCAGTAGATGCGGACTTGCTCCCAGAAGTGGTTCCTGGATTTAAGGCTCCAATTAGGCGTTGTCCACCAAACACTAGATCAAGGCTAACAGATGATGAACTAACTAACTTGAGGAAGTCCGCTCGTTCATTGCCTACTCATTTTGTCTTAGGAAGGAACAGGAAACTTCAAGGCTTGGCTGCTGCCATCTTAAAGTTGTGGGAGAAAAGTCAGATAGCAAAGATTGCTGTGAAGTTCGGAGTTCCAAATACAAACAATGAGCAAATGGCATATGAGCTGAAGTGTCTCACAGGAGGAGTTCTGTTACTGCGCAATAAGTTTATTATATTACTCTATAGAGGCAAGGACTTTCTTCCTTGTGGAGTTTCGGATTTAGTAGCAAAAAGAGAAATAGAGCTCAATAGATGGCAACTCCATGAAGAACATGCACGCCTGAAAGCAATTGAAACTTTTTCTGAAGATGATGAACCATTAGGCAGTACTGGCACAGTGGGAACATTATCAGAATTCCATAATATCCAAACAGAGTATGGAGACCTTATAAGAAGAAATAAAGACATTGAAATTAAGCTGGAAGCTGAAAAGGCACGACTGGGGAGGGAGCTGAGGAATCAAGAGCACAAAGCGTTCATTCTAAACAGAAAAATAGAGAAATCAACAAATAAGTTATCAAAGTTGAATTATCAATGGACACCGGCTGAGCAGGATGTTGACCAAGAAATGATGACTGAAGAAGAGAGAGAATGTTTCCGAAAGATAGGACTGCAGATGCATAGTTGCTTAGTACTTGGTAGGCGTGGGGTCTTTAATGGGGTAAAGGAAGGTATTCATCAGCACTGGAAGCACAGGGAGGTAGTCAAAGTTATTACAATGCAGAAACTGTTTGGACAGGTCATGTACACCGCGAAGCTTCTTGAAGCAGAAAGTGGTGGGGTTCTCGTCTCTGTGGATAAGCTAAAAAAGGGCCATGCTATTATTATCTACCGTGGGAGAAATTACCGACGGCCTTTTAAGCCAATATGTGGGAATCTTCTATCTAAAAGAAAAGCATTACATAGGTCTCTTGAAATGCAGAGAATTGGATCGCTCAAGTTTTTTGCATCCCAGAGACAGCAGGCTGCCTTAGATTTGAAACTGAAACTGGAAACTTTGCAACAAAATAGAGGAATTAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016070 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRS1_YhbY

Protein Analysis

797

Amino Acids

90.33

Weight (kDa)

9.4

Isoelectric Point (pI)

44.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRS1_YhbY	PF01985	217 - 300	7.2e-15	CRS1 / YhbY (CRM) domain
CRS1_YhbY	PF01985	434 - 518	1e-13	CRS1 / YhbY (CRM) domain
CRS1_YhbY	PF01985	647 - 734	2.5e-15	CRS1 / YhbY (CRM) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0020911)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16180 AT5G16180
malus_domestica	MD09G1254900.v1.1
prunus_persica	Prupe.3G145000_v2.0.a1
pyrus_communis	pycom09g17080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1757
Acc16I	TGCGCA	1 cut(s)	1526
AccB1I	GGYRCC	2 cut(s)	190, 942
AccB7I	CCANNNNNTGG	1 cut(s)	2237
AccBSI	CCGCTC	1 cut(s)	1337
AccII	CGCG	2 cut(s)	71, 2120
AciI	CCGC	8 cut(s)	6, 48, 137, 218, 635, 1226, 1335, 2118
AclWI	GGATC	5 cut(s)	268, 713, 1076, 1089, 2305
AcsI	RAATTY	3 cut(s)	791, 926, 1721
AcuI	CTGAAG	3 cut(s)	1514, 1692, 1965
AcyI	GRCGYC	1 cut(s)	191
AfaI	GTAC	4 cut(s)	462, 1696, 2001, 2114
AfiI	CCNNNNNNNGG	4 cut(s)	267, 416, 1243, 2237
AjiI	CACGTC	1 cut(s)	799
AjnI	CCWGG	2 cut(s)	411, 1249
Alw21I	GWGCWC	5 cut(s)	173, 780, 829, 1614, 1836
Alw26I	GTCTC	5 cut(s)	1505, 1743, 2161, 2280, 2317
AlwI	GGATC	5 cut(s)	268, 713, 1076, 1089, 2305
AlwNI	CAGNNNCTG	1 cut(s)	2095
Ama87I	CYCGRG	1 cut(s)	550
AoxI	GGCC	4 cut(s)	540, 720, 2179, 2219
ApeKI	GCWGC	5 cut(s)	197, 686, 1394, 1397, 2333
ApoI	RAATTY	3 cut(s)	791, 926, 1721
AseI	ATTAAT	1 cut(s)	2385
Asp700I	GAANNNNTTC	2 cut(s)	1245, 2040
AspLEI	GCGC	3 cut(s)	73, 193, 1527
AspS9I	GGNCC	5 cut(s)	215, 386, 1134, 1178, 2179
AsuC2I	CCSGG	3 cut(s)	279, 562, 724
AsuHPI	GGTGA	1 cut(s)	692
AsuII	TTCGAA	1 cut(s)	923
AvaI	CYCGRG	1 cut(s)	550
AvaII	GGWCC	4 cut(s)	215, 386, 1134, 1178
BamHI	GGATCC	1 cut(s)	1081
BanI	GGYRCC	2 cut(s)	190, 942
BanII	GRGCYC	5 cut(s)	173, 459, 780, 829, 1614
BbsI	GAAGAC	1 cut(s)	854
Bbv12I	GWGCWC	5 cut(s)	173, 780, 829, 1614, 1836
BbvCI	CCTCAGC	1 cut(s)	1820
BbvI	GCAGC	5 cut(s)	209, 698, 1381, 1384, 2320
BccI	CCATC	6 cut(s)	60, 89, 589, 1163, 1409, 1614
BceAI	ACGGC	3 cut(s)	559, 722, 2234
BcgI	CGANNNNNNTGC	2 cut(s)	310, 344
BciT130I	CCWGG	2 cut(s)	413, 1251
BcnI	CCSGG	3 cut(s)	279, 562, 724
BcoDI	GTCTC	5 cut(s)	1505, 1743, 2161, 2280, 2317
BfaI	CTAG	3 cut(s)	314, 1100, 1290
BfmI	CTRYAG	3 cut(s)	195, 1549, 1979
BfoI	RGCGCY	1 cut(s)	194
BglII	AGATCT	1 cut(s)	982
BisI	GCNGC	6 cut(s)	198, 636, 687, 1395, 1398, 2334
BlpI	GCTNAGC	1 cut(s)	1914
BlsI	GCNGC	6 cut(s)	199, 637, 688, 1396, 1399, 2335
BmcAI	AGTACT	2 cut(s)	1696, 2001
Bme1390I	CCNGG	5 cut(s)	279, 413, 562, 724, 1251
Bme18I	GGWCC	4 cut(s)	215, 386, 1134, 1178
BmeT110I	CYCGRG	1 cut(s)	550
BmgBI	CACGTC	1 cut(s)	799
BmgT120I	GGNCC	5 cut(s)	215, 386, 1134, 1178, 2179
BmiI	GGNNCC	7 cut(s)	192, 216, 484, 944, 1083, 1248, 1263
BmrFI	CCNGG	5 cut(s)	279, 413, 562, 724, 1251
BmrI	ACTGGG	1 cut(s)	1817
BmsI	GCATC	4 cut(s)	1191, 1213, 1974, 2324
BmuI	ACTGGG	1 cut(s)	1817
BpiI	GAAGAC	1 cut(s)	854
BpmI	CTGGAG	1 cut(s)	873
Bpu10I	CCTNAGC	2 cut(s)	1174, 1820
Bpu1102I	GCTNAGC	1 cut(s)	1914
Bpu14I	TTCGAA	1 cut(s)	923
BpuEI	CTTGAG	7 cut(s)	671, 686, 717, 813, 860, 1345, 2288
BpuMI	CCSGG	3 cut(s)	279, 562, 724
BsaHI	GRCGYC	1 cut(s)	191
BsaI	GGTCTC	2 cut(s)	1743, 2280
BsaJI	CCNNGG	6 cut(s)	203, 277, 411, 575, 723, 2200
BsaXI	ACNNNNNCTCC	2 cut(s)	846, 876
Bsc4I	CCNNNNNNNGG	4 cut(s)	267, 416, 1243, 2237
Bse118I	RCCGGY	1 cut(s)	1909
Bse1I	ACTGG	7 cut(s)	856, 1199, 1217, 1702, 1812, 2057, 2361
Bse3DI	GCAATG	2 cut(s)	289, 1343
BseBI	CCWGG	2 cut(s)	413, 1251
BseDI	CCNNGG	6 cut(s)	203, 277, 411, 575, 723, 2200
BseGI	GGATG	8 cut(s)	213, 600, 974, 1054, 1174, 1206, 1927, 2315
BseLI	CCNNNNNNNGG	4 cut(s)	267, 416, 1243, 2237
BseMI	GCAATG	2 cut(s)	289, 1343
BseMII	CTCAG	3 cut(s)	993, 1811, 1905
BseNI	ACTGG	7 cut(s)	856, 1199, 1217, 1702, 1812, 2057, 2361
BseRI	GAGGAG	1 cut(s)	1524
BseXI	GCAGC	5 cut(s)	209, 698, 1381, 1384, 2320
Bsh1236I	CGCG	2 cut(s)	71, 2120
BshFI	GGCC	4 cut(s)	542, 722, 2181, 2221
BshNI	GGYRCC	2 cut(s)	190, 942
BsiHKAI	GWGCWC	5 cut(s)	173, 780, 829, 1614, 1836
BsiHKCI	CYCGRG	1 cut(s)	550
BsiSI	CCGG	4 cut(s)	279, 561, 723, 1910
BslFI	GGGAC	1 cut(s)	543
BslI	CCNNNNNNNGG	4 cut(s)	267, 416, 1243, 2237
BsmAI	GTCTC	5 cut(s)	1505, 1743, 2161, 2280, 2317
BsmBI	CGTCTC	1 cut(s)	2161
BsmFI	GGGAC	1 cut(s)	543
BsmI	GAATGC	1 cut(s)	139
BsnI	GGCC	4 cut(s)	542, 722, 2181, 2221
Bso31I	GGTCTC	2 cut(s)	1743, 2280
BsoBI	CYCGRG	1 cut(s)	550
Bsp119I	TTCGAA	1 cut(s)	923
Bsp1286I	GDGCHC	6 cut(s)	173, 459, 780, 829, 1614, 1836
Bsp1407I	TGTACA	1 cut(s)	2112
Bsp143I	GATC	6 cut(s)	273, 705, 982, 1081, 1292, 2297
Bsp1720I	GCTNAGC	1 cut(s)	1914
Bsp19I	CCATGG	1 cut(s)	203
BspACI	CCGC	8 cut(s)	6, 48, 137, 218, 635, 1226, 1335, 2118
BspANI	GGCC	4 cut(s)	542, 722, 2181, 2221
BspCNI	CTCAG	3 cut(s)	992, 1812, 1906
BspFNI	CGCG	2 cut(s)	71, 2120
BspLI	GGNNCC	7 cut(s)	192, 216, 484, 944, 1083, 1248, 1263
BspMAI	CTGCAG	2 cut(s)	199, 1983
BspPI	GGATC	5 cut(s)	268, 713, 1076, 1089, 2305
BspT104I	TTCGAA	1 cut(s)	923
BspT107I	GGYRCC	2 cut(s)	190, 942
BspTNI	GGTCTC	2 cut(s)	1743, 2280
BsrBI	CCGCTC	1 cut(s)	1337
BsrDI	GCAATG	2 cut(s)	289, 1343
BsrFI	RCCGGY	1 cut(s)	1909
BsrGI	TGTACA	1 cut(s)	2112
BsrI	ACTGG	7 cut(s)	856, 1199, 1217, 1702, 1812, 2057, 2361
BssAI	RCCGGY	1 cut(s)	1909
BssECI	CCNNGG	6 cut(s)	203, 277, 411, 575, 723, 2200
BssMI	GATC	6 cut(s)	273, 705, 982, 1081, 1292, 2297
BssNI	GRCGYC	1 cut(s)	191
BssT1I	CCWWGG	1 cut(s)	203
Bst2UI	CCWGG	2 cut(s)	413, 1251
Bst4CI	ACNGT	3 cut(s)	1705, 2097, 2201
Bst6I	CTCTTC	2 cut(s)	20, 1944
BstACI	GRCGYC	1 cut(s)	191
BstAPI	GCANNNNNTGC	1 cut(s)	1439
BstAUI	TGTACA	1 cut(s)	2112
BstBI	TTCGAA	1 cut(s)	923
BstC8I	GCNNGC	6 cut(s)	6, 195, 780, 958, 1645, 2331
BstDEI	CTNAG	7 cut(s)	979, 1174, 1363, 1820, 1914, 1996, 2338
BstDSI	CCRYGG	3 cut(s)	203, 575, 2200
BstF5I	GGATG	8 cut(s)	213, 600, 974, 1054, 1174, 1206, 1927, 2315
BstFNI	CGCG	2 cut(s)	71, 2120
BstH2I	RGCGCY	1 cut(s)	194
BstHHI	GCGC	3 cut(s)	73, 193, 1527
BstKTI	GATC	6 cut(s)	276, 708, 985, 1084, 1295, 2300
BstMAI	GTCTC	5 cut(s)	1505, 1743, 2161, 2280, 2317
BstMBI	GATC	6 cut(s)	273, 705, 982, 1081, 1292, 2297
BstNI	CCWGG	2 cut(s)	413, 1251
BstNSI	RCATGY	1 cut(s)	1643
BstSCI	CCNGG	5 cut(s)	277, 411, 560, 722, 1249
BstSFI	CTRYAG	3 cut(s)	195, 1549, 1979
BstUI	CGCG	2 cut(s)	71, 2120
BstV1I	GCAGC	5 cut(s)	209, 698, 1381, 1384, 2320
BstV2I	GAAGAC	1 cut(s)	854
BstX2I	RGATCY	3 cut(s)	273, 982, 1081
BstYI	RGATCY	3 cut(s)	273, 982, 1081
BsuRI	GGCC	4 cut(s)	542, 722, 2181, 2221
BtgI	CCRYGG	3 cut(s)	203, 575, 2200
BtrI	CACGTC	1 cut(s)	799
BtsCI	GGATG	8 cut(s)	213, 600, 974, 1054, 1174, 1206, 1927, 2315
BtsIMutI	CAGTG	2 cut(s)	1710, 2050
Cac8I	GCNNGC	6 cut(s)	6, 195, 780, 958, 1645, 2331
CaiI	CAGNNNCTG	1 cut(s)	2095
CfoI	GCGC	3 cut(s)	73, 193, 1527
Cfr10I	RCCGGY	1 cut(s)	1909
Cfr13I	GGNCC	5 cut(s)	215, 386, 1134, 1178, 2179
CseI	GACGC	1 cut(s)	185
Csp6I	GTAC	4 cut(s)	461, 1695, 2000, 2113
CviAII	CATG	7 cut(s)	204, 784, 859, 1631, 1640, 2110, 2183
CviQI	GTAC	4 cut(s)	461, 1695, 2000, 2113
DdeI	CTNAG	7 cut(s)	979, 1174, 1363, 1820, 1914, 1996, 2338
DinI	GGCGCC	1 cut(s)	192
DpnI	GATC	6 cut(s)	275, 707, 984, 1083, 1294, 2299
DpnII	GATC	6 cut(s)	273, 705, 982, 1081, 1292, 2297
Eam1104I	CTCTTC	2 cut(s)	20, 1944
EarI	CTCTTC	2 cut(s)	20, 1944
Ecl136II	GAGCTC	4 cut(s)	171, 778, 827, 1612
Eco130I	CCWWGG	1 cut(s)	203
Eco24I	GRGCYC	5 cut(s)	173, 459, 780, 829, 1614
Eco31I	GGTCTC	2 cut(s)	1743, 2280
Eco47I	GGWCC	4 cut(s)	215, 386, 1134, 1178
Eco53kI	GAGCTC	4 cut(s)	171, 778, 827, 1612
Eco57I	CTGAAG	3 cut(s)	1514, 1692, 1965
Eco88I	CYCGRG	1 cut(s)	550
EcoICRI	GAGCTC	4 cut(s)	171, 778, 827, 1612
EcoO109I	RGGNCCY	2 cut(s)	1134, 1178
EcoRI	GAATTC	1 cut(s)	1721
EcoRII	CCWGG	2 cut(s)	411, 1249
EcoT14I	CCWWGG	1 cut(s)	203
EcoT22I	ATGCAT	2 cut(s)	1206, 1989
EcoT38I	GRGCYC	5 cut(s)	173, 459, 780, 829, 1614
EgeI	GGCGCC	1 cut(s)	192
EheI	GGCGCC	1 cut(s)	192
ErhI	CCWWGG	1 cut(s)	203
Esp3I	CGTCTC	1 cut(s)	2161
FaeI	CATG	7 cut(s)	207, 787, 862, 1634, 1643, 2113, 2186
FalI	AAGNNNNNCTT	6 cut(s)	648, 680, 822, 854, 870, 902
FaqI	GGGAC	1 cut(s)	543
FatI	CATG	7 cut(s)	203, 783, 858, 1630, 1639, 2109, 2182
FauI	CCCGC	2 cut(s)	13, 144
FauNDI	CATATG	1 cut(s)	1486
Fnu4HI	GCNGC	6 cut(s)	198, 636, 687, 1395, 1398, 2334
FokI	GGATG	8 cut(s)	220, 607, 961, 1061, 1181, 1213, 1934, 2302
FriOI	GRGCYC	5 cut(s)	173, 459, 780, 829, 1614
Fsp4HI	GCNGC	6 cut(s)	198, 636, 687, 1395, 1398, 2334
FspBI	CTAG	3 cut(s)	314, 1100, 1290
FspI	TGCGCA	1 cut(s)	1526
GlaI	GCGC	3 cut(s)	72, 192, 1526
GluI	GCNGC	6 cut(s)	198, 636, 687, 1395, 1398, 2334
GsuI	CTGGAG	1 cut(s)	873
HaeII	RGCGCY	1 cut(s)	194
HaeIII	GGCC	4 cut(s)	542, 722, 2181, 2221
HapII	CCGG	4 cut(s)	279, 561, 723, 1910
HgaI	GACGC	1 cut(s)	185
HhaI	GCGC	3 cut(s)	73, 193, 1527
Hin1I	GRCGYC	1 cut(s)	191
Hin1II	CATG	7 cut(s)	207, 787, 862, 1634, 1643, 2113, 2186
Hin6I	GCGC	3 cut(s)	71, 191, 1525
HinP1I	GCGC	3 cut(s)	71, 191, 1525
HincII	GTYRAC	1 cut(s)	1927
HindII	GTYRAC	1 cut(s)	1927
HindIII	AAGCTT	1 cut(s)	2123
HpaII	CCGG	4 cut(s)	279, 561, 723, 1910
HphI	GGTGA	1 cut(s)	692
Hpy166II	GTNNAC	3 cut(s)	1280, 1927, 2115
Hpy188III	TCNNGA	9 cut(s)	367, 401, 830, 839, 965, 1100, 1829, 2129, 2279
Hpy8I	GTNNAC	3 cut(s)	1280, 1927, 2115
Hpy99I	CGWCG	3 cut(s)	179, 800, 2220
HpyAV	CCTTC	7 cut(s)	205, 410, 712, 760, 1141, 1362, 2030
HpyCH4III	ACNGT	3 cut(s)	1705, 2097, 2201
HpyCH4IV	ACGT	1 cut(s)	798
HpyF3I	CTNAG	7 cut(s)	979, 1174, 1363, 1820, 1914, 1996, 2338
HpySE526I	ACGT	1 cut(s)	798
Hsp92I	GRCGYC	1 cut(s)	191
Hsp92II	CATG	7 cut(s)	207, 787, 862, 1634, 1643, 2113, 2186
HspAI	GCGC	3 cut(s)	71, 191, 1525
KasI	GGCGCC	1 cut(s)	190
Kzo9I	GATC	6 cut(s)	273, 705, 982, 1081, 1292, 2297
LmnI	GCTCC	6 cut(s)	176, 205, 310, 1239, 1267, 1816
Lsp1109I	GCAGC	5 cut(s)	209, 698, 1381, 1384, 2320
LweI	GCATC	4 cut(s)	1191, 1213, 1974, 2324
MaeI	CTAG	3 cut(s)	314, 1100, 1290
MaeII	ACGT	1 cut(s)	798
MaeIII	GTNAC	2 cut(s)	727, 1518
MalI	GATC	6 cut(s)	275, 707, 984, 1083, 1294, 2299
MbiI	CCGCTC	1 cut(s)	1337
MboI	GATC	6 cut(s)	273, 705, 982, 1081, 1292, 2297
MfeI	CAATTG	1 cut(s)	1656
MflI	RGATCY	3 cut(s)	273, 982, 1081
MhlI	GDGCHC	6 cut(s)	173, 459, 780, 829, 1614, 1836
Mly113I	GGCGCC	1 cut(s)	191
MlyI	GAGTC	4 cut(s)	115, 167, 542, 875
MmeI	TCCRAC	1 cut(s)	198
Mph1103I	ATGCAT	2 cut(s)	1206, 1989
MroXI	GAANNNNTTC	2 cut(s)	1245, 2040
MspA1I	CMGCKG	1 cut(s)	994
MspI	CCGG	4 cut(s)	279, 561, 723, 1910
MspR9I	CCNGG	5 cut(s)	279, 413, 562, 724, 1251
MunI	CAATTG	1 cut(s)	1656
Mva1269I	GAATGC	1 cut(s)	139
MvaI	CCWGG	2 cut(s)	413, 1251
MvnI	CGCG	2 cut(s)	71, 2120
NarI	GGCGCC	1 cut(s)	191
NciI	CCSGG	3 cut(s)	279, 562, 724
NcoI	CCATGG	1 cut(s)	203
NdeI	CATATG	1 cut(s)	1486
NdeII	GATC	6 cut(s)	273, 705, 982, 1081, 1292, 2297
NlaIII	CATG	7 cut(s)	207, 787, 862, 1634, 1643, 2113, 2186
NlaIV	GGNNCC	7 cut(s)	192, 216, 484, 944, 1083, 1248, 1263
NmuCI	GTSAC	1 cut(s)	727
NsbI	TGCGCA	1 cut(s)	1526
NsiI	ATGCAT	2 cut(s)	1206, 1989
NspI	RCATGY	1 cut(s)	1643
NspV	TTCGAA	1 cut(s)	923
PaeR7I	CTCGAG	1 cut(s)	550
PctI	GAATGC	1 cut(s)	139
PdmI	GAANNNNTTC	2 cut(s)	1245, 2040
PfeI	GAWTC	6 cut(s)	305, 397, 835, 1185, 1825, 2242
PflMI	CCANNNNNTGG	1 cut(s)	2237
PfoI	TCCNGGA	2 cut(s)	560, 1249
PkrI	GCNGC	6 cut(s)	199, 637, 688, 1396, 1399, 2335
PleI	GAGTC	4 cut(s)	115, 166, 542, 875
PluTI	GGCGCC	1 cut(s)	194
PpsI	GAGTC	4 cut(s)	115, 166, 542, 875
PpuMI	RGGWCCY	2 cut(s)	1134, 1178
PshBI	ATTAAT	1 cut(s)	2385
PsiI	TTATAA	1 cut(s)	1757
Psp124BI	GAGCTC	4 cut(s)	173, 780, 829, 1614
Psp5II	RGGWCCY	2 cut(s)	1134, 1178
Psp6I	CCWGG	2 cut(s)	411, 1249
PspGI	CCWGG	2 cut(s)	411, 1249
PspN4I	GGNNCC	7 cut(s)	192, 216, 484, 944, 1083, 1248, 1263
PspPI	GGNCC	5 cut(s)	215, 386, 1134, 1178, 2179
PspPPI	RGGWCCY	2 cut(s)	1134, 1178
PspXI	VCTCGAGB	1 cut(s)	550
PstI	CTGCAG	2 cut(s)	199, 1983
PstNI	CAGNNNCTG	1 cut(s)	2095
PsuI	RGATCY	3 cut(s)	273, 982, 1081
PvuII	CAGCTG	1 cut(s)	994
RsaI	GTAC	4 cut(s)	462, 1696, 2001, 2114
RsaNI	GTAC	4 cut(s)	461, 1695, 2000, 2113
SacI	GAGCTC	4 cut(s)	173, 780, 829, 1614
SatI	GCNGC	6 cut(s)	198, 636, 687, 1395, 1398, 2334
Sau3AI	GATC	6 cut(s)	273, 705, 982, 1081, 1292, 2297
Sau96I	GGNCC	5 cut(s)	215, 386, 1134, 1178, 2179
ScaI	AGTACT	2 cut(s)	1696, 2001
SchI	GAGTC	4 cut(s)	115, 167, 542, 875
ScrFI	CCNGG	5 cut(s)	279, 413, 562, 724, 1251
SduI	GDGCHC	6 cut(s)	173, 459, 780, 829, 1614, 1836
SfaNI	GCATC	4 cut(s)	1191, 1213, 1974, 2324
SfcI	CTRYAG	3 cut(s)	195, 1549, 1979
SfoI	GGCGCC	1 cut(s)	192
Sfr274I	CTCGAG	1 cut(s)	550
SfuI	TTCGAA	1 cut(s)	923
SinI	GGWCC	4 cut(s)	215, 386, 1134, 1178
SlaI	CTCGAG	1 cut(s)	550
SmlI	CTYRAG	8 cut(s)	550, 650, 665, 732, 828, 839, 1324, 2303
SmoI	CTYRAG	8 cut(s)	550, 650, 665, 732, 828, 839, 1324, 2303
SsiI	CCGC	8 cut(s)	6, 48, 137, 218, 635, 1226, 1335, 2118
SspDI	GGCGCC	1 cut(s)	190
SspMI	CTAG	3 cut(s)	314, 1100, 1290
SstI	GAGCTC	4 cut(s)	173, 780, 829, 1614
StyD4I	CCNGG	5 cut(s)	277, 411, 560, 722, 1249
StyI	CCWWGG	1 cut(s)	203
TaaI	ACNGT	3 cut(s)	1705, 2097, 2201
TaiI	ACGT	1 cut(s)	801
TaqI	TCGA	5 cut(s)	167, 551, 795, 923, 1183
TatI	WGTACW	3 cut(s)	1694, 1999, 2112
TauI	GCSGC	1 cut(s)	638
TfiI	GAWTC	6 cut(s)	305, 397, 835, 1185, 1825, 2242
TscAI	CASTG	2 cut(s)	1710, 2057
TseFI	GTSAC	1 cut(s)	727
TseI	GCWGC	5 cut(s)	197, 686, 1394, 1397, 2333
Tsp45I	GTSAC	1 cut(s)	727
TspGWI	ACGGA	1 cut(s)	172
TspRI	CASTG	2 cut(s)	1710, 2057
Van91I	CCANNNNNTGG	1 cut(s)	2237
VpaK11BI	GGWCC	4 cut(s)	215, 386, 1134, 1178
VspI	ATTAAT	1 cut(s)	2385
XapI	RAATTY	3 cut(s)	791, 926, 1721
XbaI	TCTAGA	1 cut(s)	1099
XceI	RCATGY	1 cut(s)	1643
XcmI	CCANNNNNNNNNTGG	4 cut(s)	151, 419, 494, 1742
XhoI	CTCGAG	1 cut(s)	550
XmnI	GAANNNNTTC	2 cut(s)	1245, 2040
XspI	CTAG	3 cut(s)	314, 1100, 1290
ZrmI	AGTACT	2 cut(s)	1696, 2001
Zsp2I	ATGCAT	2 cut(s)	1206, 1989

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.