MD09G1287500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0028475
Physical Location & Seq
Reverse (-)
36750571 .. 36752491
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1287500.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGTTGAAAGGATTAGAACACATAACTTTCCAAGTTGTAGAAACCAAAACGGATTGGGTCGATGCAAAGTGCCTTGCAGGAGACGGAATTCACCTCAAGGTGCTAGAAAATGAAGTGCTTCTCAAGATGATCAATGAGATGCTGAAGGGACTGACGACGGGAATGACAGAGAGGATTGCTGGATATATACTTATACTTGTGTGGAACATGAGGTCCGCATCTGACAACAGTAGGCGCATCTTTCCGGAAAAGAGCCCAAGTAATCAAGGGATAGATGTTGAGGCCCTTTCTGACGGAGTTGTATCACCCCGAGGGACAACCCCCATCAAGCTTTCTGCAGCAGGGGGAAACAGTTATTCCCTAAATGAGTGTGTCAGAGTTGGATGTTTTCTAGCTGCCTCTCTGTTAAAATTGTTCAGAAAACCTGCATACTCTTGGGACAAAGCCAAGTATCATATACAGAGAGCGTACACTAACTATACTAAGGAACCGTGGCCATTGAAAGACCTCAATGTCATCGCCAGTCATGTTGGAACACTGTCTCTTTTATGGGAACGTAAGACCATATTCAGGGACACCCTGGGATCCATTCTGTACTCCATAGGAGACCTTGAAGAGGGTCGAGGGATGGTTACAATGCTCTTTGCAGGGCATCTTGCATACACTGGAATGCACGTTGTCGATTTGTTCTGCAGAGCTGTAACAGGTCTTCAGTGTCAACCGACTGATTTGATGAAAGCCTTACATACACATACACATACATCCATGCGTAAAAAGAGTATTCTTGACGAGCTAAGAATTATAATTGAATCATATATAGGGTCGGAGGAAGAAGACAAAAAAAGACGGACGTTCCAGTATGCAAGACTGTTTGACAGTCAAATGTTCGGTAATCTACAATCGAAGAATTGTGCCCATCTAGTCTGTATCTTAGCATACCTTTGTCAACAATGTGGAAGTGCAGGAGCCGGAGACGCAACCCAAATTGCAGTGATAGCAAACATGGGGCAAGGGGCCAAGGACAATTGCAAGGCCGAAGCAATCAACATCTACAATTATATCGTTACCAAGAATGAAATAGAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

363

Amino Acids

40.38

Weight (kDa)

8.42

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhabdo_ncap_2 PF03216 27 - 314 2.1e-51 Rhabdovirus nucleoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028475)

Species Orthologous Gene IDs
malus_domestica MD09G1287500.v1.1
pyrus_communis pycom09g19500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 803
AasI GACNNNNNNGTC 1 cut(s) 512
Acc36I ACCTGC 1 cut(s) 433
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 1 cut(s) 216
AclWI GGATC 2 cut(s) 579, 592
AcoI YGGCCR 1 cut(s) 494
AcsI RAATTY 1 cut(s) 87
AcuI CTGAAG 2 cut(s) 164, 695
AfaI GTAC 2 cut(s) 470, 596
AfiI CCNNNNNNNGG 2 cut(s) 570, 616
AgsI TTSAA 4 cut(s) 7, 502, 614, 809
AjnI CCWGG 1 cut(s) 579
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 4 cut(s) 331, 395, 698, 793
AluI AGCT 4 cut(s) 331, 395, 698, 793
Alw26I GTCTC 4 cut(s) 75, 546, 600, 966
AlwI GGATC 2 cut(s) 579, 592
Ama87I CYCGRG 1 cut(s) 309
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 4 cut(s) 282, 494, 1014, 1032
ApeKI GCWGC 2 cut(s) 338, 395
ApoI RAATTY 1 cut(s) 87
Asp700I GAANNNNTTC 1 cut(s) 117
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 3 cut(s) 213, 283, 1014
AsuHPI GGTGA 2 cut(s) 83, 297
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 1 cut(s) 213
BaeGI GKGCMC 1 cut(s) 916
BalI TGGCCA 1 cut(s) 496
BamHI GGATCC 1 cut(s) 584
BanII GRGCYC 1 cut(s) 257
BarI GAAGNNNNNNTAC 2 cut(s) 693, 725
BbsI GAAGAC 2 cut(s) 701, 840
BbvI GCAGC 2 cut(s) 350, 382
BccI CCATC 3 cut(s) 332, 622, 924
BciT130I CCWGG 1 cut(s) 581
BclI TGATCA 1 cut(s) 129
BcoDI GTCTC 4 cut(s) 75, 546, 600, 966
BfaI CTAG 3 cut(s) 104, 392, 920
BfmI CTRYAG 2 cut(s) 336, 691
BfuAI ACCTGC 1 cut(s) 433
BisI GCNGC 2 cut(s) 339, 396
BlsI GCNGC 2 cut(s) 340, 397
Bme1390I CCNGG 1 cut(s) 581
Bme18I GGWCC 1 cut(s) 213
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 3 cut(s) 213, 283, 1014
BmiI GGNNCC 4 cut(s) 489, 586, 967, 1015
BmrFI CCNGG 1 cut(s) 581
BmsI GCATC 5 cut(s) 52, 129, 227, 246, 661
BpiI GAAGAC 2 cut(s) 701, 840
BpuEI CTTGAG 2 cut(s) 80, 107
BsaI GGTCTC 1 cut(s) 600
BsaJI CCNNGG 5 cut(s) 310, 491, 579, 580, 1017
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 2 cut(s) 570, 616
Bse1I ACTGG 3 cut(s) 522, 670, 856
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 5 cut(s) 310, 491, 579, 580, 1017
BseGI GGATG 3 cut(s) 389, 633, 761
BseLI CCNNNNNNNGG 2 cut(s) 570, 616
BseNI ACTGG 3 cut(s) 522, 670, 856
BseSI GKGCMC 1 cut(s) 916
BseXI GCAGC 2 cut(s) 350, 382
BsgI GTGCAG 1 cut(s) 981
BshFI GGCC 4 cut(s) 284, 496, 1016, 1034
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 2 cut(s) 245, 969
BslFI GGGAC 4 cut(s) 162, 328, 452, 587
BslI CCNNNNNNNGG 2 cut(s) 570, 616
BsmAI GTCTC 4 cut(s) 75, 546, 600, 966
BsmBI CGTCTC 2 cut(s) 75, 966
BsmFI GGGAC 4 cut(s) 162, 328, 452, 587
BsmI GAATGC 1 cut(s) 675
BsnI GGCC 4 cut(s) 284, 496, 1016, 1034
Bso31I GGTCTC 1 cut(s) 600
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 257, 916
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 2 cut(s) 129, 584
BspACI CCGC 1 cut(s) 216
BspANI GGCC 4 cut(s) 284, 496, 1016, 1034
BspEI TCCGGA 1 cut(s) 244
BspLI GGNNCC 4 cut(s) 489, 586, 967, 1015
BspMAI CTGCAG 2 cut(s) 340, 695
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 2 cut(s) 579, 592
BspTNI GGTCTC 1 cut(s) 600
BsrI ACTGG 3 cut(s) 522, 670, 856
BssECI CCNNGG 5 cut(s) 310, 491, 579, 580, 1017
BssMI GATC 2 cut(s) 129, 584
BssT1I CCWWGG 1 cut(s) 1017
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 6 cut(s) 230, 353, 492, 540, 870, 878
Bst6I CTCTTC 1 cut(s) 609
BstDEI CTNAG 3 cut(s) 483, 794, 931
BstDSI CCRYGG 1 cut(s) 491
BstENI CCTNNNNNAGG 1 cut(s) 614
BstF5I GGATG 3 cut(s) 389, 633, 761
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 2 cut(s) 132, 587
BstMAI GTCTC 4 cut(s) 75, 546, 600, 966
BstMBI GATC 2 cut(s) 129, 584
BstMWI GCNNNNNNNGC 2 cut(s) 974, 995
BstNI CCWGG 1 cut(s) 581
BstSCI CCNGG 1 cut(s) 579
BstSFI CTRYAG 2 cut(s) 336, 691
BstSLI GKGCMC 1 cut(s) 916
BstV1I GCAGC 2 cut(s) 350, 382
BstV2I GAAGAC 2 cut(s) 701, 840
BstX2I RGATCY 1 cut(s) 584
BstYI RGATCY 1 cut(s) 584
BsuRI GGCC 4 cut(s) 284, 496, 1016, 1034
BtgI CCRYGG 1 cut(s) 491
BtgZI GCGATG 1 cut(s) 502
BtsCI GGATG 3 cut(s) 389, 633, 761
BtsI GCAGTG 1 cut(s) 996
BtsIMutI CAGTG 4 cut(s) 536, 663, 719, 996
BveI ACCTGC 1 cut(s) 433
CfoI GCGC 1 cut(s) 237
Cfr13I GGNCC 3 cut(s) 213, 283, 1014
CseI GACGC 1 cut(s) 983
Csp6I GTAC 2 cut(s) 469, 595
CviAII CATG 4 cut(s) 208, 527, 766, 1003
CviQI GTAC 2 cut(s) 469, 595
DdeI CTNAG 3 cut(s) 483, 794, 931
DpnI GATC 2 cut(s) 131, 586
DpnII GATC 2 cut(s) 129, 584
DrdI GACNNNNNNGTC 1 cut(s) 512
DseDI GACNNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 494
Eam1104I CTCTTC 1 cut(s) 609
EarI CTCTTC 1 cut(s) 609
Eco130I CCWWGG 1 cut(s) 1017
Eco24I GRGCYC 1 cut(s) 257
Eco31I GGTCTC 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 213
Eco57I CTGAAG 2 cut(s) 164, 695
Eco88I CYCGRG 1 cut(s) 309
EcoNI CCTNNNNNAGG 1 cut(s) 614
EcoO109I RGGNCCY 1 cut(s) 283
EcoRI GAATTC 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 579
EcoT14I CCWWGG 1 cut(s) 1017
EcoT38I GRGCYC 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 1017
Esp3I CGTCTC 2 cut(s) 75, 966
FaeI CATG 4 cut(s) 211, 530, 769, 1006
FaqI GGGAC 4 cut(s) 162, 328, 452, 587
FatI CATG 4 cut(s) 207, 526, 765, 1002
FbaI TGATCA 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 339, 396
FokI GGATG 3 cut(s) 396, 640, 748
FriOI GRGCYC 1 cut(s) 257
Fsp4HI GCNGC 2 cut(s) 339, 396
FspBI CTAG 3 cut(s) 104, 392, 920
GlaI GCGC 1 cut(s) 236
GluI GCNGC 2 cut(s) 339, 396
HaeIII GGCC 4 cut(s) 284, 496, 1016, 1034
HapII CCGG 2 cut(s) 245, 969
HgaI GACGC 1 cut(s) 983
HhaI GCGC 1 cut(s) 237
Hin1II CATG 4 cut(s) 211, 530, 769, 1006
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 2 cut(s) 719, 947
HindII GTYRAC 2 cut(s) 719, 947
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 1 cut(s) 809
HpaII CCGG 2 cut(s) 245, 969
HphI GGTGA 2 cut(s) 83, 297
Hpy166II GTNNAC 3 cut(s) 471, 719, 947
Hpy188I TCNGA 5 cut(s) 223, 292, 377, 419, 826
Hpy188III TCNNGA 3 cut(s) 124, 245, 785
Hpy8I GTNNAC 3 cut(s) 471, 719, 947
Hpy99I CGWCG 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 6 cut(s) 230, 353, 492, 540, 870, 878
HpyCH4IV ACGT 3 cut(s) 556, 675, 851
HpyF10VI GCNNNNNNNGC 2 cut(s) 974, 995
HpyF3I CTNAG 3 cut(s) 483, 794, 931
HpySE526I ACGT 3 cut(s) 556, 675, 851
Hsp92II CATG 4 cut(s) 211, 530, 769, 1006
HspAI GCGC 1 cut(s) 235
Kpn2I TCCGGA 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 129
Kzo9I GATC 2 cut(s) 129, 584
LmnI GCTCC 1 cut(s) 965
Lsp1109I GCAGC 2 cut(s) 350, 382
LweI GCATC 5 cut(s) 52, 129, 227, 246, 661
MaeI CTAG 3 cut(s) 104, 392, 920
MaeII ACGT 3 cut(s) 556, 675, 851
MaeIII GTNAC 3 cut(s) 631, 700, 1063
MalI GATC 2 cut(s) 131, 586
MboI GATC 2 cut(s) 129, 584
MboII GAAGA 5 cut(s) 626, 701, 842, 845, 916
MfeI CAATTG 1 cut(s) 1024
MflI RGATCY 1 cut(s) 584
MhlI GDGCHC 2 cut(s) 257, 916
MlsI TGGCCA 1 cut(s) 496
MluCI AATT 8 cut(s) 87, 410, 798, 804, 907, 984, 1024, 1054
MluNI TGGCCA 1 cut(s) 496
MmeI TCCRAC 3 cut(s) 361, 511, 804
Mox20I TGGCCA 1 cut(s) 496
MroI TCCGGA 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 117
MscI TGGCCA 1 cut(s) 496
MseI TTAA 1 cut(s) 407
MslI CAYNNNNRTG 1 cut(s) 668
Msp20I TGGCCA 1 cut(s) 496
MspI CCGG 2 cut(s) 245, 969
MspR9I CCNGG 1 cut(s) 581
MunI CAATTG 1 cut(s) 1024
Mva1269I GAATGC 1 cut(s) 675
MvaI CCWGG 1 cut(s) 581
MwoI GCNNNNNNNGC 2 cut(s) 974, 995
NdeII GATC 2 cut(s) 129, 584
NlaIII CATG 4 cut(s) 211, 530, 769, 1006
NlaIV GGNNCC 4 cut(s) 489, 586, 967, 1015
PasI CCCWGGG 1 cut(s) 580
PctI GAATGC 1 cut(s) 675
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 1 cut(s) 809
PkrI GCNGC 2 cut(s) 340, 397
PsiI TTATAA 1 cut(s) 803
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PspN4I GGNNCC 4 cut(s) 489, 586, 967, 1015
PspPI GGNCC 3 cut(s) 213, 283, 1014
PstI CTGCAG 2 cut(s) 340, 695
PsuI RGATCY 1 cut(s) 584
RsaI GTAC 2 cut(s) 470, 596
RsaNI GTAC 2 cut(s) 469, 595
RseI CAYNNNNRTG 1 cut(s) 668
SaqAI TTAA 1 cut(s) 407
SatI GCNGC 2 cut(s) 339, 396
Sau3AI GATC 2 cut(s) 129, 584
Sau96I GGNCC 3 cut(s) 213, 283, 1014
ScrFI CCNGG 1 cut(s) 581
SduI GDGCHC 2 cut(s) 257, 916
SfaNI GCATC 5 cut(s) 52, 129, 227, 246, 661
SfcI CTRYAG 2 cut(s) 336, 691
SinI GGWCC 1 cut(s) 213
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 2 cut(s) 95, 122
SmoI CTYRAG 2 cut(s) 95, 122
Sse9I AATT 8 cut(s) 87, 410, 798, 804, 907, 984, 1024, 1054
SsiI CCGC 1 cut(s) 216
SspMI CTAG 3 cut(s) 104, 392, 920
StyD4I CCNGG 1 cut(s) 579
StyI CCWWGG 1 cut(s) 1017
TaaI ACNGT 6 cut(s) 230, 353, 492, 540, 870, 878
TaiI ACGT 3 cut(s) 559, 678, 854
TaqI TCGA 4 cut(s) 60, 622, 681, 902
TasI AATT 8 cut(s) 87, 410, 798, 804, 907, 984, 1024, 1054
TatI WGTACW 1 cut(s) 594
TfiI GAWTC 1 cut(s) 809
Tru1I TTAA 1 cut(s) 407
Tru9I TTAA 1 cut(s) 407
TscAI CASTG 4 cut(s) 543, 670, 719, 996
TseI GCWGC 2 cut(s) 338, 395
TspDTI ATGAA 3 cut(s) 126, 749, 1089
TspGWI ACGGA 4 cut(s) 65, 99, 309, 862
TspRI CASTG 4 cut(s) 543, 670, 719, 996
VpaK11BI GGWCC 1 cut(s) 213
XagI CCTNNNNNAGG 1 cut(s) 614
XapI RAATTY 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 3 cut(s) 104, 392, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.