Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0028179

Physical Location & Seq

Genomic Coordinates Forward (+)

6294288 .. 6294868

Gene Structure 581 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1047200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 288 bp

ATGTCTACATATAGGTTACTTTTCTTGTGTACTTTCACTGTTTCTAATCTTGGGATGTTTGGTGTTGATCGGTTTGATGCTATTCTGCCACCTGGAACTGGAGCAATTATGGCTGTTGGATCATCTCAGCCTACTGTTGTGGCTACCAAGGATGGTCGGATTGGACGGAAGAACCAAATGCAGGTAAATGTTACTGCTGATCATCGTATAATTTATGGCGCTGATCTGGCTTCATTCTTGCAAACCTTGGCCAAGATTATTGAGGACCCCAAAGATCTTACCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005886 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016020 GO:0019904 GO:0022626 GO:0031967 GO:0031975 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0071944 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

96

Amino Acids

10.34

Weight (kDa)

7.91

Isoelectric Point (pI)

29.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-oxoacid_dh	PF00198	11 - 93	5.2e-26	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0028179)

Species	Orthologous Gene IDs
malus_domestica	MD10G1047200.v1.1
pyrus_communis	pycom10g03330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	172
AccI	GTMKAC	1 cut(s)	5
AclWI	GGATC	1 cut(s)	127
AcoI	YGGCCR	1 cut(s)	249
AfaI	GTAC	1 cut(s)	31
AfiI	CCNNNNNNNGG	2 cut(s)	98, 181
AjnI	CCWGG	1 cut(s)	91
AlwI	GGATC	1 cut(s)	127
AoxI	GGCC	1 cut(s)	249
AspLEI	GCGC	1 cut(s)	221
AspS9I	GGNCC	1 cut(s)	265
AvaII	GGWCC	1 cut(s)	265
BalI	TGGCCA	1 cut(s)	251
BccI	CCATC	1 cut(s)	146
BciT130I	CCWGG	1 cut(s)	93
BclI	TGATCA	1 cut(s)	199
BfaI	CTAG	1 cut(s)	286
BfoI	RGCGCY	1 cut(s)	222
BfuAI	ACCTGC	1 cut(s)	172
BglII	AGATCT	1 cut(s)	274
Bme1390I	CCNGG	1 cut(s)	93
Bme18I	GGWCC	1 cut(s)	265
BmgT120I	GGNCC	1 cut(s)	265
BmiI	GGNNCC	1 cut(s)	267
BmrFI	CCNGG	1 cut(s)	93
BmsI	GCATC	1 cut(s)	67
BpmI	CTGGAG	1 cut(s)	120
BsaJI	CCNNGG	2 cut(s)	147, 246
Bsc4I	CCNNNNNNNGG	2 cut(s)	98, 181
Bse1I	ACTGG	1 cut(s)	103
BseBI	CCWGG	1 cut(s)	93
BseDI	CCNNGG	2 cut(s)	147, 246
BseGI	GGATG	2 cut(s)	60, 157
BseLI	CCNNNNNNNGG	2 cut(s)	98, 181
BseMII	CTCAG	1 cut(s)	140
BseNI	ACTGG	1 cut(s)	103
BshFI	GGCC	1 cut(s)	251
BslI	CCNNNNNNNGG	2 cut(s)	98, 181
BsnI	GGCC	1 cut(s)	251
Bsp143I	GATC	5 cut(s)	67, 119, 199, 223, 274
BspANI	GGCC	1 cut(s)	251
BspCNI	CTCAG	1 cut(s)	139
BspLI	GGNNCC	1 cut(s)	267
BspMI	ACCTGC	1 cut(s)	172
BspPI	GGATC	1 cut(s)	127
BsrI	ACTGG	1 cut(s)	103
BssECI	CCNNGG	2 cut(s)	147, 246
BssMI	GATC	5 cut(s)	67, 119, 199, 223, 274
BssT1I	CCWWGG	2 cut(s)	147, 246
Bst2UI	CCWGG	1 cut(s)	93
Bst4CI	ACNGT	2 cut(s)	40, 136
BstDEI	CTNAG	1 cut(s)	126
BstF5I	GGATG	2 cut(s)	60, 157
BstH2I	RGCGCY	1 cut(s)	222
BstHHI	GCGC	1 cut(s)	221
BstKTI	GATC	5 cut(s)	70, 122, 202, 226, 277
BstMBI	GATC	5 cut(s)	67, 119, 199, 223, 274
BstMWI	GCNNNNNNNGC	2 cut(s)	110, 227
BstNI	CCWGG	1 cut(s)	93
BstSCI	CCNGG	1 cut(s)	91
BstX2I	RGATCY	1 cut(s)	274
BstYI	RGATCY	1 cut(s)	274
BsuRI	GGCC	1 cut(s)	251
BtsCI	GGATG	2 cut(s)	60, 157
BtsIMutI	CAGTG	1 cut(s)	36
BveI	ACCTGC	1 cut(s)	172
CfoI	GCGC	1 cut(s)	221
Cfr13I	GGNCC	1 cut(s)	265
Csp6I	GTAC	1 cut(s)	30
CviJI	RGCY	5 cut(s)	113, 130, 143, 230, 251
CviKI_1	RGCY	5 cut(s)	113, 130, 143, 230, 251
CviQI	GTAC	1 cut(s)	30
DdeI	CTNAG	1 cut(s)	126
DpnI	GATC	5 cut(s)	69, 121, 201, 225, 276
DpnII	GATC	5 cut(s)	67, 119, 199, 223, 274
EaeI	YGGCCR	1 cut(s)	249
Eco130I	CCWWGG	2 cut(s)	147, 246
Eco47I	GGWCC	1 cut(s)	265
EcoO109I	RGGNCCY	1 cut(s)	265
EcoRII	CCWGG	1 cut(s)	91
EcoT14I	CCWWGG	2 cut(s)	147, 246
ErhI	CCWWGG	2 cut(s)	147, 246
FaiI	YATR	5 cut(s)	10, 12, 110, 209, 216
FbaI	TGATCA	1 cut(s)	199
FblI	GTMKAC	1 cut(s)	5
FokI	GGATG	2 cut(s)	67, 164
FspBI	CTAG	1 cut(s)	286
GlaI	GCGC	1 cut(s)	220
GsuI	CTGGAG	1 cut(s)	120
HaeII	RGCGCY	1 cut(s)	222
HaeIII	GGCC	1 cut(s)	251
HhaI	GCGC	1 cut(s)	221
Hin6I	GCGC	1 cut(s)	219
HinP1I	GCGC	1 cut(s)	219
Hpy166II	GTNNAC	2 cut(s)	6, 30
Hpy188I	TCNGA	1 cut(s)	159
Hpy8I	GTNNAC	2 cut(s)	6, 30
HpyCH4III	ACNGT	2 cut(s)	40, 136
HpyCH4V	TGCA	2 cut(s)	181, 241
HpyF10VI	GCNNNNNNNGC	2 cut(s)	110, 227
HpyF3I	CTNAG	1 cut(s)	126
HspAI	GCGC	1 cut(s)	219
Ksp22I	TGATCA	1 cut(s)	199
Kzo9I	GATC	5 cut(s)	67, 119, 199, 223, 274
LmnI	GCTCC	1 cut(s)	101
LpnPI	CCDG	5 cut(s)	78, 84, 105, 167, 212
LweI	GCATC	1 cut(s)	67
MaeI	CTAG	1 cut(s)	286
MaeIII	GTNAC	2 cut(s)	15, 190
MalI	GATC	5 cut(s)	69, 121, 201, 225, 276
MboI	GATC	5 cut(s)	67, 119, 199, 223, 274
MboII	GAAGA	1 cut(s)	181
MflI	RGATCY	1 cut(s)	274
MlsI	TGGCCA	1 cut(s)	251
MluCI	AATT	2 cut(s)	105, 210
MluNI	TGGCCA	1 cut(s)	251
MmeI	TCCRAC	2 cut(s)	97, 137
MnlI	CCTC	1 cut(s)	256
Mox20I	TGGCCA	1 cut(s)	251
MscI	TGGCCA	1 cut(s)	251
Msp20I	TGGCCA	1 cut(s)	251
MspR9I	CCNGG	1 cut(s)	93
MvaI	CCWGG	1 cut(s)	93
MwoI	GCNNNNNNNGC	2 cut(s)	110, 227
NdeII	GATC	5 cut(s)	67, 119, 199, 223, 274
NlaIV	GGNNCC	1 cut(s)	267
PpuMI	RGGWCCY	1 cut(s)	265
Psp5II	RGGWCCY	1 cut(s)	265
Psp6I	CCWGG	1 cut(s)	91
PspGI	CCWGG	1 cut(s)	91
PspN4I	GGNNCC	1 cut(s)	267
PspPI	GGNCC	1 cut(s)	265
PspPPI	RGGWCCY	1 cut(s)	265
PsuI	RGATCY	1 cut(s)	274
RsaI	GTAC	1 cut(s)	31
RsaNI	GTAC	1 cut(s)	30
Sau3AI	GATC	5 cut(s)	67, 119, 199, 223, 274
Sau96I	GGNCC	1 cut(s)	265
ScrFI	CCNGG	1 cut(s)	93
SetI	ASST	5 cut(s)	17, 94, 186, 248, 284
SfaNI	GCATC	1 cut(s)	67
SinI	GGWCC	1 cut(s)	265
Sse9I	AATT	2 cut(s)	105, 210
SspMI	CTAG	1 cut(s)	286
StyD4I	CCNGG	1 cut(s)	91
StyI	CCWWGG	2 cut(s)	147, 246
TaaI	ACNGT	2 cut(s)	40, 136
TasI	AATT	2 cut(s)	105, 210
TatI	WGTACW	1 cut(s)	29
TscAI	CASTG	1 cut(s)	43
TspDTI	ATGAA	1 cut(s)	222
TspGWI	ACGGA	1 cut(s)	181
TspRI	CASTG	1 cut(s)	43
VpaK11BI	GGWCC	1 cut(s)	265
XmiI	GTMKAC	1 cut(s)	5
XspI	CTAG	1 cut(s)	286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.